KR101880991B1 - Antimicrobial composition against propionibacterium acne and pathogenic microorganism - Google Patents

Abstract

The present invention relates to an anti-bacterial composition against causative bacteria of acne and pathogenic microorganisms. The anti-bacterial composition of the present invention comprises components of an extract of 2.0 to 3.0 wt % of Phellodendri Cortex, 1.0 to 3.0 wt % of Scutellaria baicalensis, 1.0 to 3.0 wt % of Moutan Cortex, 1.0 to 3.0 wt % of liquorice, 3.0 to 3.5 wt % of Sanguisorba Officinalis Root, and 2.0 to 3.0 wt % of myrobalan, and acts on bacteria selected from the group consisting of Propionibacterium acnes, Candida albicas, Aspergillus fumigatus, Escherichia coli, Staphylococcus aureus, and Corynebacterium amycolatum.

Description

[0001] The present invention relates to an antimicrobial composition against acne causative bacteria and harmful microorganisms,

The present invention relates to a composition having excellent antimicrobial activity against various harmful microorganisms including Propionibacterium acnes , which is known as a causative organism of acne.

Acne is called pimple or acne vulgaris. It is a chronic inflammatory disease of the epidermis. It is mainly produced during adolescence, and many intrinsic and extrinsic factors are complexly combined to form the cause of the disease.

The pathogenesis of acne has not yet been clarified and is reported to be caused by various factors. The cause of acne is thought to be mainly the increase of sebaceous glands, abnormal keratinization of wall follicles, bacterial growth and inflammation. One of the most important features of acne is the increase in sebum secretion, and the increase in sebum secretion is closely related to the male hormone, androgens. It is the initial stage of acne which is caused by the stagnation of hair in the hair follicle due to the keratinization of the hair follicles. On the other hand, there is a change in the lipid component of sebum in acne patients. The content of linoleic acid and cholesterol in acyl ceramide is decreased and the content of squalene and free fatty acid is increased. These changes in fat composition result in an imbalance between cholesterol and cholesterol sulfate in the hair follicle, which enhances the association between keratinocytes in the hair follicle and results in hair follicle hyperkeratosis. As a result, stagnant sebum in the hair follicles blocks the air circulation by blocking the hair follicles, so that various bacteria grow around the hair follicle and the inflammation reaction occurs.

Common treatments for acne include topical treatments, phototherapy and photodynamic therapy, physical therapy, and systemic treatment.

Topical treatments include antibiotics, benzoyl peroxide, retinoids, etc. These topical treatments have side effects of dry skin, pruritus, erythema and photosensitivity.

Phototherapy is the action of sterilization by activating the porphyrin produced by the acne bacterium by irradiating the light, and the photodynamic therapy is to sterilize the light by applying the light sensitizer. However, since acne is not the only acne bacterium before the occurrence of acne, there are patients who do not improve with phototherapy and photodynamic therapy, and acne may be temporarily aggravated, erythema and skin irritation.

Physical treatments include cotton extrusion and peeling. Surgical extrusion is a surgical suture that does not fundamentally resolve the pathology of acne, so repeated extrusion is necessary.

Peeling can be caused by skin irritation, pain and scarring as a treatment to apply chemicals, heat with a laser, or mechanically peel off the epidermis or dermis.

Systemic therapies include antibiotics, retinoids, and antihistamines. However, the use of antihormonal agents inhibits the growth of the epidermis, hepatotoxicity, thrombosis, and side effects of embolization. In the case of antibiotics, the possibility of bleeding and photosensitization of resistant bacteria emerged. Oral retinoids are the only agents that affect major acne pathogenic factors, but they are associated with serious side effects, especially severe ones.

Therefore, unlike the treatments listed above, it is necessary to seek treatment that can improve or cure acne without side effects.

Accordingly, when treating acne using a composition using a natural extract, it is impossible to reveal the exact mechanism, but there are no side effects or side effects, and excellent therapeutic effects can be obtained, and various applications using natural extracts have been attempted.

For example, Patent Document 10-2012-0138066 discloses an agent for treating acne using natural extracts such as ginseng, garlic, gugija, sookiji, black bean, and the like. In the patent 10-1135172, Patent Document 10-0791420 discloses a pharmaceutical composition for treating acne and atopy using a mugwort component having an antibacterial action, and Patent Document 10-0981038 discloses a pharmaceutical composition for treating acne, There is disclosed a composition for treating atopic and acne comprising at least one herbal medicine extract, such as Sukjunghwang, Seokgwang, Seokwon, Golden, Pruning, Persimmon, Licorice, Peony root,

Thus, conventional acne remedies have disadvantages such as appearance of resistant bacteria, photosensitivity, skin irritation, and teratogenicity as described above. However, acne treatments using the extracts of yellowish white, gold, mulberry, licorice, The composition for treatment and prevention has not found such side effects.

The object of the present invention is not only to effectively inhibit the growth and proliferation of Propionibacterium acnes causing acne but also to prevent Candida albicas , Aspergillus fumigatus , Escherichia coli , The present invention also provides a composition having excellent antimicrobial activity against harmful microorganisms including Staphylococcus aureus and Corynebacterium amycolatum .

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In order to achieve the above object, the present invention provides a process for producing a fermented soybean oil comprising 2.0 to 3.0% by weight of yellowish white, 1.0 to 3.0% by weight of gold, 1.0 to 3.0% by weight of liana, 1.0 to 3.0% by weight of licorice, 3.0 to 3.5% % Of an extract component and is selected from the group consisting of Propionibacterium acnes fungi, Candida albicas fungi, Aspergillus fumigatus fungi, Escherichia coli , Staphylococcus aureus , and Corynebacterium amycolatum . The present invention also relates to a method for producing a microorganism, which comprises culturing a microorganism selected from the group consisting of Staphylococcus aureus and Corynebacterium amycolatum .
Here, the bacterium is preferably Propionibacterium acnes .

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Hereinafter, the present invention will be described in detail.

The present invention contains a natural composition having excellent antimicrobial activity against Propionibacterium acnes , which is known to be a major cause of acne. In addition, the use of a liquid preparation containing the composition in a skin patch designed to act effectively on a localized area effectively transfers the active ingredient for a long time, and the skin patch gives excellent adhesion when in contact with the skin. The composition is characterized in that it contains components of yellowish white, gold, liquorice, licorice, papaya, and gherkin extract. The present invention relates to a skin adhesive agent and a liquid agent having high efficacy in which a drug substance is delivered for a longer period of time by using a liquid agent mixed therewith rather than using only a skin adhesive agent.

The present invention relates to an anti-acne composition comprising an adhesive layer which adheres to the skin and which maintains the characteristics of the adhesive layer and maintains the shape of the adhesive layer and the support layer located at the lower end of the adhesive layer, A skin adhesive agent composed of a release agent; And a patch for preventing and treating acne characterized by comprising a liquid agent comprising an anti-acne composition, wherein a groove for administering the liquid agent is formed on the adhesive layer of the skin adhesive agent.

In the patch for preventing and treating acne according to the present invention, the groove may be modified into various forms, but it is preferably circular, and the support layer of the skin adhesive agent is made of a solution containing ethyl cellulose, castor oil and ethanol And the adhesive layer of the skin adhesive agent is preferably made of a solution containing polyvinylpyrrolidone, carbopol, glycerin, hydroxyethylcellulose, ethanol, fragrance and water in addition to the anti-acne composition, In addition to the anti-acne composition it is further preferred to be made with a solution comprising glycerin, dipropylene glycol, polyoxyethylene hydrogenated castor oil, carboxymethylcellulose and water.

For the prevention and treatment of acne according to the present invention, the composition preferably contains the components of yellowish white, gold, liquorice, licorice, papaya and gad extract, wherein 2.0-3.0% by weight of yellowish white, 1.0-3.0% It is more preferable that the extract be 1.0 to 3.0% by weight of the rhizome, 1.0 to 3.0% by weight of the licorice, 3.0 to 3.5% by weight of the fat and 2.0 to 3.0% by weight of the potato.

In addition, the composition of the present invention may be used in combination with other bacteria such as Propionibacterium acnes strain, Candida albicas strain, Aspergillus fumigatus strain, Escherichia coli strain, Staphylococcus aureus strain, Staphylococcus aureus , and Corynebacterium amycolatum , and more preferably those acting on Propionibacterium acnes . In particular, it is preferable that the bacteria act on a bacterium selected from the group consisting of Staphylococcus aureus , Corynebacterium amycolatum , desirable.

The characteristics and extraction method of each component contained in the composition will be described below.

≪ Herbal medicine materials and extraction method >

Yellow, white, gold, mulberry, licorice, papaya, and Gadja extracts were extracted using conventional extraction methods. For example, yellowish white, gold, liquorice, liquorice, oilseed oil and lettuce can be used after filtration after stirring for 3 days at room temperature (15 ~ 25 ℃) using 70% ethanol extraction method. The contents of indicator substances and heavy metals were confirmed based on the test method of Korea Food and Drug Administration.

(1) Yellowish white

The rustaceae and the bark of the whitewood or other inbred plants belonging to the family are also called dried flowers, and the scientific name is Phellodendron amurense Rupr. It is used for inflammation, pneumonia, bone tuberculosis, cold, sterilization medicine, anti-inflammatory drug in the oriental medicine, and it is also used as a pharmacotherapeutic drug for other infectious diseases such as conjunctivitis, gonad salt, chronic colitis, Lee, JG, et al., Separation of melanin biosynthesis inhibitor from 黄柏, Korean Society of Pharmaceutical Sciences 38 (4), 387-393, 2007).

(2) Golden (黄 芩, Scutellaria Root)

The spiny grass belonging to Lamiaceae and Labiatae is the root of Scutellaria baicalensis Georgi as it is or has been removed. The shape is circular, semi-tubular or flat, 5 ~ 25㎝ in length and 5 ~ 30㎜ in diameter. There is little smell and taste a little bit. The main ingredients are Baicalein, Baicalin, Wogonin, Wogonoside, Neobaicalein, and β-Sitosterol. The antimicrobial effect, anti-cancer, anti-inflammation, ejaculation, hypotension, and diuretic effect are known as pharmacological actions.

(3) Moutan Root Bark

Paeonia suffruticosa is a root shell of Andrews (peonies and Paeoniaceae). Its shape is cylindrical or semicylindrical, 5 ~ 20㎝ in length, 0.5 ~ 1.2cm in diameter, and 0.1 ~ 0.4cm in thickness. There is a peculiar smell and the taste is a bit spicy. The main ingredients are Paeoniflorin, Oxypaeoniflorin, Paeonol, Paeonolide, Paeonoside, and Tannin. Antimicrobial effects, antioxidant, anti-inflammatory, antiviral and anticonvulsive effects are known as pharmacological actions.

(4) licorice

Leguminosae is a perennial herb that belongs to the genus Glycyrrhiza uralensis Fisch. It contains glycyrrhizin, a sweet component, and is used as a sweetener. It has a therapeutic effect against various diseases, antibacterial action and excellent antioxidant ability. It is known to act on the heart, lungs, and gastric glands. It is known to be effective against ejang, wet, and livestock. It is used as an antidote to poisoning, and is used as an antipyretic agent and emollient. In addition, pain relief, weight gain, leukocyte increase, diuretic action, and anti-inflammatory action are known to be caused by sudden tense muscle or tissue (antimicrobial and antioxidant ability of water extract of licorice and spice, , ≪ / RTI > 793-799, 2007).

(5)

It is a perennial plant belonging to the rose family, which is the root part of the oat. It is distributed in Korea, China, Japan, Russia, etc. It is a wild plant growing in mountains and fields. For a long time, it has been used as an agent for detoxification, burning, diarrhea, respiratory and gastrointestinal diseases. Recently, various uses such as anti-allergy, anti-cancer, Generally, methanol, ethanol, or water is used as a solvent for extracting plants. The efficacy of the hydrothermal extract of JiHu has been reported to be useful. Anticancer effect that inhibits cell proliferation of oral cancer And immunopotentiation have been reported in previous studies. The hydrothermal extraction method can extract natural substances easily compared with other methods and can obtain a large amount of useful components. Since the extraction method does not use a chemical component such as ethanol and the like, the efficacy of the extract is low due to low toxicity, It can be said that it is safe. In addition, Jiyu is a herb that grows in the mountains and has the advantage of being easy to cultivate, high self-sustaining power, and easy to obtain.

The present invention also relates to a method for preparing a skin adhesive composition, comprising the steps of: 1) attaching an adhesive layer and a support layer to the skin in the skin adhesive agent; And 2) administering a solution containing the anti-acne composition to the groove of the skin adhesive agent.

According to the present invention configured as described above, it is possible to effectively prevent the growth and proliferation of Propionibacterium acnes causing acne, thereby preventing and / or improving acne. In addition, it has been reported that a harmful microorganism selected from the group consisting of Candida albicas , Aspergillus fumigatus , Escherichia coli , Staphylococcus aureus , and Corynebacterium amycolatum The antibacterial effect is excellent.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a front view of the skin adhesive agent of the present invention. Fig.
2 is a side view of the skin adhesive agent of the present invention.
At this time, the support layer was prepared by making 100% of ethylcellulose with 16.0% of ethylcellulose and 5.0% of castor oil, followed by coating and drying. The adherent layer was made of polyvinylpyrrolidone 13.0%, carbopol 0.05%, glycerin 3.0% Water was added to 0.2% of hydroxyethylcellulose, 2.0% of ethanol, 0.1% of fragrance, and 10.0% of anti-acne composition (extract of Example 15) to make 100%, followed by coating and drying. The support layer solution prepared according to the prescription was coated on a polyethylene film to a thickness of 20 탆, and then an adhesive layer solution was applied on the polyethylene film to form a skin adhesive agent.
3 is a schematic view of the liquid agent used in the present invention. 1 to 2 drops of the liquid agent can be adhered to the acne area in the circular groove at the center of the skin adhesives shown in Figs.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments thereof. However, it should be understood that the following embodiments are provided so that those skilled in the art may understand the present invention without departing from the scope and spirit of the present invention. It is not.

EXAMPLES The strains used, culture medium and culture conditions

The strains used in the present invention are Propionibacterium acnes KCTC3314, Escherichia coli KCTC2571, Candida albicans KCTC7965, Aspergillus fumigatus KCTC26426, Staphylococcus aureus KCTC1621 and Corynebacterium amycolatum KCTC 3432, which are causative agents of acne, Respectively. Characteristics of the characteristics of the above strains are shown in Table 1 below.

KCTC number Strain name Badge name Incubation temperature Pre-sale type 3314 Propionibacterium acnes RC 37 ℃ Ampoule 2571 Escherichia coli NB 37 ℃ Ampoule 7965 Candida albicans YM 25 ℃ Ampoule 26426 Aspergillus fumigatus PDB 25 ℃ Ampoule 1621 Staphylococcus aureus MSB 37 ℃ Ampoule 3432 Corynebacterium amycolatum NB 37 ℃ Ampoule

Each of the above strains was diluted to 500 μl in sterilized water and then lyophilized (8 kinds) from the microorganism resource center of Korea were diluted in solid medium and cultured according to the growth conditions of each strain. Activated three days ago. The anaerobic bacterium was inoculated on a culture medium, sealed with a gaspack system (BD GasPak ™ EZ Anaerobe Gas Generating Pouch System with Indicator) in an anaerobic jar, and anaerobically incubated in an incubator at 37 ° C. for 3 days.

≪ Preparation Example 1 > A skin extract and a natural extract applied to a liquid preparation

In order to confirm the antimicrobial synergistic activity of the natural extracts applied to the skin adhesive agent and the liquid agent of the present invention, the extracts of yellow, white, gold, wood, licorice, Respectively.

<Test Example> Measurement of antimicrobial activity against bacteria

The antimicrobial activity was evaluated using a paper disc diffusion method. Specifically, the antimicrobial activity measurement using the disk diffusion method is as follows. Each strain was cultured for 3 days in accordance with the growth conditions. The suspension was adjusted to a constant concentration (optical density at 620 nm, absorbance value 0.5) using a spreader (HYUNDAI Micro Co., LTD.), And then uniformly plated on an agar plate. After drying the plate, a paper disc (Φ 10 mm, Adventec. USA) was placed on the surface of the inoculated plate and the natural extract was absorbed by 200 μl / disc. The plate treated with the sample was incubated for 3 days under the growth conditions of each strain, and the antimicrobial activity was measured to the size of the inhibition zone around the disk.

&Lt; Test Example 1 > Antibacterial synergy test of natural extract applied to skin adhesive agent and liquid agent

From the above production examples, the antibacterial activity of each mixture was tested according to Paper Disc Test. The strains used here are Corynebacterium amycolatum and Propionibacterium acnes .

extract Yellowish white Gold Mural licorice Oil Go P. acnes strain Antibacterial activity Antimicrobial activity of C. amycolatum strain Example 1 ○ ○ ○ ○ ○ ○ ++++ ++++ Example 2 ○ ○ ○ ○ ○ +++ ++ Example 3 ○ ○ ○ ○ ○ ++ ++++ Example 4 ○ ○ ○ ○ ○ ++++ ++ Example 5 ○ ○ ○ ○ ++ ++ Example 6 ○ ○ ○ ○ +++ ++ Example 7 ○ ○ ○ ○ ++ +++ Example 8 ○ ○ ○ ++ ++ Example 9 ○ ++ ++ Example 10 ○ - + Example 11 ○ + ++ Example 12 ○ - ++ Example 13 ○ +++ + Example 14 ○ + +++

Remark) ++++: 25mm, +++: 20mm, ++: 15mm, +: 10mm, -: No antibacterial power

Table 2 shows that the extract of P. japonica has excellent antimicrobial activity against P. acnes and the antioxidative activity of C. amycolatum strain is superior to other extracts of Gadja extract. Further, when assuming its activity in P.acnes strains and C.amycolatum hayeoteul mixed with the antibacterial activity is less than or was not yellowish, golden, mokdanpi, licorice extract and the P.acnes C.amycolatum strain or a synergistic effect that increases the activity can see.

In Example 9-14 of Table 2, each of the natural products was put into a single unit and tested for its antimicrobial activity. In Example 8, three kinds of yellowish white, golden, and herring extracts were mixed. In Example 5-7, yellowish white, , Licorice, papaya, and GAZA extract were added to the mixture, and then the mixture was tested. Example 5 was prepared by mixing 5 kinds of yellowish white, golden, woody, jade and ghazu extracts, and Example 2-3 was prepared by mixing 5 kinds of yellowish white, gold, , And Example 1 was tested by mixing all six kinds of yellowish white, gold, liquorice, licorice, crude oil and ghatti extract. Examples 1 and 2 showed the best antimicrobial activity in P. acnes strains and excellent antimicrobial activity in C. amycolatum strains in Examples 1 and 3.

From the above results, it was confirmed that the synergistic effect of antimicrobial activity can be most excellently produced when 6 kinds of the extracts of yellow, white, gold, mulberry, licorice, papaya and gherkin are mixed.

&Lt; Preparation Example 2 > A skin extract and a natural extract applied to a liquid preparation

In order to confirm the antimicrobial effect when the natural compositions to be applied to the skin adhesive agent and the liquid agent of the present invention were prepared by the ratio, the yellowish white, gold, mulberry, licorice,

extract Example 15 Example 16 Example 17 Example 18 Example 19 Yellowish white 2.0% 1.0% 0.5% 3.0% 2.0% Gold 1.0% 1.5% 2.5% 0.3% 3.0% Mural 1.0% 3.0% 0.5% 2.0% 1.0% licorice 1.0% 3.0% 1.0% 1.7% 0.5% Oil 3.0% 1.0% 3.5% 2.0% 0.5% Go 2.0% 0.5% 2.0% 1.0% 3.0%

&Lt; Example 15 >

The natural composition was prepared by adding distilled water to 2.0% of whitish white, 1.0% of gold, 1.0% of licorice, 1.0% of licorice, 3.0% of crude oil and 2.0% of Gaza.

&Lt; Example 16 >

The natural composition was prepared by adding distilled water to 1.0% of yellowish white, 1.5% of gold, 3.0% of licorice, 3.0% of licorice, 1.0% of crude oil and 0.5% of Gaza.

&Lt; Example 17 >

The natural composition was prepared by adding distilled water to 0.5% of white gold, 2.5% of gold, 0.5% of licorice, 1.0% of licorice, 3.5% of crude oil and 2.0% of Gaza.

&Lt; Example 18 >

The natural composition was prepared by adding distilled water to 3.0% of yellowish white, 0.3% of gold, 2.0% of sesame oil, 2.0% of licorice, 1.7% of ground oil, 2.0% of ground oil and 1.0% of green tea.

&Lt; Example 19 >

The natural composition was prepared by adding distilled water to 2.0% of whitish white, 3.0% of gold, 1.0% of licorice, 0.5% of licorice, 0.5% of crude oil and 3.0% of GAD.

<Test Example 2> Antimicrobial activity test of a natural composition applied to a skin adhesive agent and a liquid agent

The antibacterial activity of each mixture was tested according to the Paper Disc Test from the natural compositions prepared from the above Preparation Examples. The strains used in this study were Propionibacterium acnes , Candida albicas , Aspergillus fumigatus , Escherichia coli , Staphylococcus aureus , Skin and mucosal pathogens Corynebacterium amycolatum ).

Strain Example 15 Example 16 Example 17 Example 18 Example 19 A.fumigatus + - - + + C.albicans + + + - + E. coli + + - + + S.aureus + + + + - C. amycolatum + - + - + P.acnes + - + - -

Remarks) +: Has antibacterial activity, -: Has no antibacterial activity

As a result, as shown in Table 4, Example 15 shows antibacterial activity in all six strains. Example 16 showed that the antimicrobial activity of A. fumigatus was not exhibited when the content of the extract was lower than 2.0% and the antimicrobial activity was not found in the P. acnes strain when the extract was lower than 3.0% Indicating that there is no antimicrobial activity in C. amycolatum strains when lowered. Example 17 shows that there was no antimicrobial activity in the E.coli strain when the herringbone extract was lowered to less than 1.0% and that the A.fumigatus strain had no antimicrobial activity when the content of the extract was lower than that of Example 15. [ Example 18 showed that the antimicrobial activity was not found in the C. albicans strain when the gold extract was lower than 1.0% and the antimicrobial activity was lower in the C. amycolatum and P. acnes strains when the content of the crude oil extract and the potato extract was smaller than that of Example 15 None. Example 19 shows that when the licorice extract was lowered to less than 1.0%, there was no antimicrobial activity in the S. aureus strain, and when the content of the crude oil extract was smaller than that of Example 15, the antimicrobial activity was not found in the P. acnes strain.

In other words, The content of the extract of P. japonica should be 3.0%, so that the P. acnes strain has an antibacterial activity and the content of Gadja extract should be 2.0%, indicating that the C. amycolatum strain has antibacterial activity. In addition, the content of the extracts of yellow, white, gold, mulberry and licorice extracts should be as much as the content of Example 15, indicating that the antifungal activity of A. fumigatus, C. albicans, E. coli, and S. aureus is effective.

&Lt; Test Example 3 > A comparative test with a typical preservative commonly used in cosmetics

The antimicrobial activity measurement was evaluated using a paper disc diffusion method. The strains used were: Propionibacterium acnes , Candida albicas , Aspergillus fumigatus , Escherichia coli , Staphylococcus aureus , Skin and mucosal pathogens Corynebacterium amycolatum ).

As a positive control, methylparaben was used as a typical preservative commonly used in cosmetics.

Strain Natural composition Methyl paraben One A.fumigatus + + 2 C.albicans + - 3 E. coli + + 4 S.aureus + + 5 C. amycolatum + - 6 P.acnes + -

Remarks) +: Has antibacterial activity, -: Has no antibacterial activity

As shown in Table 5, the natural composition of the present invention has antimicrobial activity against Candida albicans , Corynebacterium amycolatum , and Propionibacterium acnes , which are different from the positive control group, for mucocutaneous candidiasis have. Particularly, it was confirmed that the present invention has the ability to inhibit the growth of Propionibacterium acnes , which is known as the causative agent of acne.

The following is a specific formulation example of a skin adhesive agent and a liquid agent containing the natural composition of the present invention.

&Lt; Example 20 > Preparation of skin adhesion agent 1

The support layer solution was composed of 16.0% of ethylcellulose and 5.0% of castor oil, and ethanol was added to make 100%. The adherent layer solution was prepared by adding water to 13.0% of polyvinylpyrrolidone, 0.05% of carbopol, 3.0% of glycerin, 0.2% of hydroxyethylcellulose, 2.0% of ethanol, 0.1% of fragrance and 10.0% Respectively. The support layer solution prepared according to the prescription was coated on a polyethylene film to a thickness of 20 탆, and then an adhesive layer solution was applied on the polyethylene film to form a skin adhesive agent.

Example 21 Production of Liquid Solution 1

Water was added to 5% of glycerin, 3.0% of dipropylene glycol, 0.4% of polyoxyethylene hydrogenated castor oil, 0.3% of carboxymethylcellulose, and 10% of the extract of Example 15 to prepare a liquid agent at 100%.

&Lt; Example 22 > Preparation of skin adhesion agent and liquid agent

The support layer solution of the skin adhesive agent was made up of 16.0% of ethyl cellulose and 5.0% of castor oil and 100% by adding ethanol. The adherent layer solution was prepared by adding water to 13.0% of polyvinylpyrrolidone, 0.05% of carbopol, 3.0% of glycerin, 0.2% of hydroxyethylcellulose, 2.0% of ethanol, 0.1% of fragrance and 10.0% Respectively. The support layer solution prepared according to the prescription was applied to a polyethylene film to a thickness of 20 탆, and then an adhesive layer solution was applied thereon and dried to a thickness of 100 탆 to prepare a skin adhesive agent.

The liquid agent was prepared by adding water to 5% of glycerin, 3.0% of dipropylene glycol, 0.4% of polyoxyethylene hydrogenated castor oil, 0.3% of carboxymethylcellulose, and 10% of the extract of Example 15 to make 100%.

A skin adhesive agent (Example 20) and a liquid agent (Example 21) were used in combination.

&Lt; Comparative Example 1 > Preparation of skin adhesion agent

The support layer solution was composed of 16.0% of ethylcellulose and 5.0% of castor oil, and ethanol was added to make 100%. The adherent layer solution was made up to 100% by adding water to 13.0% of polyvinylpyrrolidone, 0.05% of carbopol, 3.0% of glycerin, 0.2% of hydroxyethylcellulose, 2.0% of ethanol and 0.1% of fragrance. The support layer solution prepared according to the prescription was applied to a polyethylene film to a thickness of 20 탆, and then an adhesive layer solution was applied thereon and dried to a thickness of 100 탆 to prepare a skin adhesive agent.

&Lt; Comparative Example 2 > Preparation of solution

Water was added to 5% of glycerin, 3.0% of dipropylene glycol, 0.4% of polyoxyethylene hydrogenated castor oil, and 0.3% of carboxymethylcellulose to prepare a liquid agent at 100%.

<Test Example 4>

In order to compare the effects of alleviating acne, 30 skin test agents and liquid preparations of Examples 20, 21, 22, and Comparative Examples 1 and 2 were recruited and 30 skin test agents and solutions were tested. Experiments were conducted in accordance with experimental conditions, such as using the same cosmetics and washing the same, inducing similar meals during the experiment.

In Table 5, it was confirmed that the use of the skin adhesive agent and the liquid agent of the present invention in combination provides a very good degree of mitigation of acne. In addition, the effect of alleviating acne within 3 days can be seen until 25 days. As a result, it was confirmed that Example 22 using the skin adhesive agent and the liquid agent in combination had better results than those of Example 20 and Example 21 using the skin adhesive agent and the liquid agent, respectively. Comparative Examples 1 and 2 were found to be ineffective as a skin adhesive agent and a liquid agent that did not contain a natural composition developed by the Company.

Treatment period Example 20 Example 21 Example 22 Comparative Example 1 Comparative Example 2 3 days 60 55 75 21 21 10 days 63 59 79 23 22 17th 68 62 82 23 21 25th 70 65 90 25 21

Remarks) Evaluation Criteria

80 ~ 100: Very effective for alleviating acne

60 ~ 79: Acne relief is good

40 ~ 59: Acne Reduction Effect

20 ~ 39: Acne relief and no side effects

0 ~ 19: Side effects available

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, This is possible.

Claims (9)

A composition for antimicrobial action against acne causing bacteria and harmful microorganisms,
The composition according to any one of claims 1 to 3, which contains an extract component of 2.0-3.0% by weight of yellowish white, 1.0-3.0% by weight of gold, 1.0-3.0% by weight of liquorice, 1.0-3.0% by weight of licorice, 3.0-3.5%
For example, Propionibacterium acnes bacteria, Candida albicas bacteria, Aspergillus fumigatus bacteria, Escherichia coli , Staphylococcus aureus bacteria, And Corynebacterium amycolatum . The composition according to claim 1, wherein the antimicrobial agent is selected from the group consisting of acyclovir and corynebacterium amycolatum . The method according to claim 1,
Wherein the bacterium is Propionibacterium acnes . 2. The composition according to claim 1, wherein the bacterium is Propionibacterium acnes . delete delete delete delete delete delete delete

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