KR101841204B1 - Nanoemulsion composition containing fermented oil, preparation method thereof, and cosmetic composition containing the same - Google Patents

Abstract

Provided are nanoemulsion composition containing fermented oil, a preparation method thereof, and a cosmetic composition containing the same. According to the present invention, fermented oil having excellent skin absorbing power and moisturizing power are included in the nanoemulsion, so when the nanoemulsion is applied to cosmetics, metastability can be improved and skin penetration and skin moisturization of active ingredients can be improved.

Description

TECHNICAL FIELD The present invention relates to a nanoemulsion composition containing a fermentation oil, a method for producing the same, and a cosmetic composition containing the fermented oil,

A fermentation oil, a method for producing the same, and a cosmetic composition containing the same.

In order to impart skin moisturizing and softening effect, vegetable oils and hydrocarbon oils are used as cosmetic ingredients. In recent years, due to the problem of skin irritation and troubles, it has been aimed at vegetable oils obtained from natural plants rather than synthetic hydrocarbons and ester oils. However, when a large amount of vegetable oil is used, there is a disadvantage that it has a negative effect such as slow absorption, stickiness, and shine. To overcome the disadvantage of such vegetable oil, fermentation oil can be used. The fermented oil is fermented by microorganisms, and the content of essential fatty acids and free fatty acids is remarkably higher than that of general oils, so that it has good skin absorbency and moisturizing power. However, it contains many unsaturated fatty acids such as linolenic acid and oleic acid and is sensitive to oxidative stress and discoloration .

The oil soluble ingredient is encapsulated in an emulsion and the water soluble ingredient is encapsulated in liposomes for skin aging, whitening, skin soothing, etc. through the improvement of the skin stratum corneum through the moisturizing effect of the skin, (Korean Patent Laid-Open No. 10-2003-0065176, Korean Patent No. 10-2003-0037106, etc.). Emulsions or liposomes are generally used in water-in-oil type, oil-in-water type and water-in-oil type. -in-oil-in-water type) are used. These vesicles are characterized by intercellular lipid and structurally It can be said that some of the ingredients are able to absorb the skin because they are similar. However, since the particle size is the smallest micro-unit, the area of contact of the component with the skin is relatively small and the similarity of the lipid composition is deteriorated, so that there is a problem in obtaining the component transfer efficiency enough to obtain the desired effect. Even when the size of the existing nanoemulsion is nano-sized using a high-pressure emulsifier, the lipid membrane itself becomes too rigid due to the formation of lipid membranes using phospholipids and ceramides, and skin adsorption is difficult, which limits the effective delivery of the components .

Therefore, there is a need to develop an emulsion that has metastability when applying fermented oil to a cosmetic composition and can effectively penetrate the skin barrier to improve skin penetration.

A nanoemulsion composition comprising a fermentation oil is provided.

There is provided a method of preparing a nanoemulsion composition comprising a fermentation oil.

A cosmetic composition comprising a nanoemulsion composition comprising a fermented oil.

One aspect is a nanoemulsion composition comprising a fermentation oil comprising: a lipid concentrate comprising at least one selected from the group consisting of a polyhydric alcohol, a phospholipid, a ceramide, and a glycolipid; An oil phase comprising at least one member selected from the group consisting of fermentation oils and antioxidants; And a water phase comprising at least one selected from the group consisting of a moisturizing agent and a polymer gelling agent.

The term "nanoemulsion" refers to a liquid in which two or more liquids that do not dissolve one another are dispersed in small particles on the other side, and the size of the particles is in the nanometer size. The nanoemulsion may have a multi-membrane or multi-layer structure. The multi-membrane structure may be a double lipid bilayer in one nanoemulsion particle.

The term "fermented oil" refers to an oil obtained through a fermentation process. The fermented oil may be one in which the oil is fermented by a microorganism. The fermentation oil may be one in which at least one oil selected from the group consisting of argan oil, olive oil, grape seed oil, rice germ oil, sunflower seed oil, and canola oil is fermented. The fermented oil is oil soluble, and it has higher content of essential fatty acid or free fatty acid than general natural oil, and thus can be excellent in skin hygroscopicity, moisturizing property, or softening effect. The fermentation oil may be located in the oil phase of the lipid membrane particles (e.g., the interface in the lipid bilayer), the innermost lipid phase of the multiple membranes, or a combination thereof.

The term " polyhydric alcohol "refers to an alcohol having two or more hydroxyl groups (-OH) per molecule. The polyhydric alcohol is referred to as a divalent alcohol, a trivalent alcohol, a tetravalent alcohol or the like depending on the number of hydroxyl groups. The polyhydric alcohol may include at least one selected from the group consisting of butylene glycol, propylene glycol, glycerin, pentylene glycol, dipropylene glycol, and diglycerin. The polyhydric alcohol may be a mixture of two or more of the above compounds. The polyhydric alcohol can increase the efficiency of dissolving lipids in water.

The term "phospholipid" refers to an ester compound of phosphoric acid. The phospholipid may function as a solubilizer of the fermentation oil. The phospholipid can dissolve an excess amount of fermentation oil almost transparently. The phospholipid may be a saturated lipid. The phospholipid may be a glycerophospholipid or a sphingolipid. The phospholipid may include one or more selected from the group consisting of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and phosphatic acid. The phospholipid can be a mixture of two or more of the compounds. The phospholipid may be lecithin (e.g., soybean lecithin).

The term "ceramide" refers to a compound consisting of sphingosine and a fatty acid, also referred to as N-acylsphingosine. Ceramides are the main component of the stratum corneum of the epidermal layer of the skin. The ceramide may include one or more selected from the group consisting of ceramide EOP, ceramide NS, ceramide NP, ceramide AS, and ceramide AP. The ceramide can fill in the phospholipids in the particle walls of one aspect of the nanoemulsion and can form a solid particle wall of the nanoemulsion.

The term "glycolipid" refers to a complex lipid in which a sugar such as a glucoside or galactose is bonded to a lipid moiety. The glycolipid may be glycolipid with sphingoglycolipids or glycerides. The glycolipid may be a fermented glycolipid. The fermented glycolipids may be the glycolipids obtained through the fermentation process. The fermented glycolipids may include one or more selected from the group consisting of rice germ oil, argan oil, grape seed oil, sunflower seed oil, and canola oil. The glycolipid imparts fluidity to the interface membrane, so that the flexible particle wall can pass through the horny layer of the skin to facilitate the transfer of the fermentation oil.

The term " antioxidant "refers to a substance that prevents the aging of cells or the oxidation of cells by removing active oxygen that damages the cells. The antioxidant may provide an antioxidant effect or a skin improving effect. The antioxidant may include tocopherol, a tocopherol derivative (e.g., tocopheryl acetate), a vitamin C lipophilic derivative, or a combination thereof.

The term "moisturizer" refers to a substance that softens and moisturizes the skin by inhibiting drying of the skin to enhance the skin's appearance. The moisturizing agent is, for example, panthenol, glycerin, hyaluronic acid, sodium hyaluronate, shea butter, and vitamin E.

The term "gelling agent" refers to a material that solidifies a liquid into a gel state. The polymeric gelling agent may be selected from the group consisting of inulin lauryl carbamate, polyquaternium-51, mucin, mucopolysaccharide, and biosaccharide gum. And the like. The polymer gelling agent is effective not only in the stability of the nano emulsion but also in the destruction of particles, Or a combination thereof.

Wherein the nanoemulsion comprises from about 20.0% to about 92.0% by weight of a lipid thickener; From about 5.0% to about 41.0% by weight of an oily phase; And from about 1.0% to about 42.0% by weight of the water phase.

Wherein the lipid concentrate comprises from about 20.0 wt% to about 80.0 wt% polyalcohol, from about 1.0 wt% to about 6.0 wt% phospholipid, from about 0.1 wt% to about 1.0 wt% ceramide, and from about 0.5 wt% to about 5.0 wt% % ≪ / RTI > glycolipids.

Wherein the lipid concentrate comprises from about 20.0 wt% to about 80.0 wt%, from about 25.0 wt% to about 70.0 wt%, from about 30.0 wt% to about 60.0 wt%, from about 30.0 wt% to about 50.0 wt%, from about 30.0 wt% 40.0 wt%, or about 35.0 wt% to about 40.0 wt% of a polyhydric alcohol.

The lipid concentrate may comprise from about 1.0 wt% to about 6.0 wt%, from about 2.0 wt% to about 6.0 wt%, from about 3.0 wt% to about 5.0 wt%, or about 4.0 wt% phospholipid. If the content of phospholipid is less than the above-mentioned content, it may be difficult to nanoemulsify the fermented oil. When the content of the phospholipid is larger than the above-mentioned content, the phospholipid itself is difficult to dissolve and the viscosity thereof is high, so that the treatment of the high-pressure emulsifier may be difficult.

Wherein the lipid concentrate comprises from about 0.1 wt% to about 1.0 wt%, from about 0.2 wt% to about 1.0 wt%, from about 0.3 wt% to about 1.0 wt%, from about 0.4 wt% to about 1.0 wt%, from about 0.5 wt% From about 0.6 wt% to about 1.0 wt%, from about 0.7 wt% to about 1.0 wt%, from about 0.7 wt% to about 0.9 wt%, or about 0.8 wt% of ceramide. If the content of ceramides is less than the above-mentioned content, it may be difficult to form a solid lipid film because the role of glue filling up the phospholipids of the nanoemulsion is insignificant. If the content of the ceramide is higher than the above-mentioned content, it may be difficult to dissolve the ceramide and the high crystallinity of the ceramide may be eliminated.

Wherein the lipid concentrate comprises from about 0.5 wt% to about 5.0 wt%, from about 1.0 wt% to about 5.0 wt%, from about 2.0 wt% to about 5.0 wt%, from about 3.0 wt% to about 5.0 wt%, or from about 4.0 wt% About 5.0% by weight of glycolipids. If the glycolipid content is less than the above-mentioned content, it is difficult to impart flexibility to the formed lipid membrane, so that it may be difficult to form a particle size smaller than the skin gap and the similarity with the skin composition may be lost. You may not be able to help. When the glycolipid is larger than the above-mentioned content, the flexibility of the lipid membrane is excessively excessive, so that the protective ability of the fermented oil, which is an active substance encapsulated in the inner vessel, is lowered and the stability may be lowered.

The oily portion may comprise from about 5.0% to about 40.0% by weight of fermentation oil, from about 0.1% to about 1% by weight of an antioxidant, or a combination thereof.

About 20.0 wt.% To about 40.0 wt.%, From about 25.0 wt.% To about 35.0 wt.%, And from about 10.0 wt.% To about 40.0 wt. By weight, or from about 30.0% to about 40.0% by weight fermentation oils.

The oily portion may comprise from about 0.1 wt% to about 1 wt%, from about 0.5 wt% to about 1 wt%, or about 1 wt% of an antioxidant.

The water-pervious portion may comprise about 2.0 wt% to about 6.0 wt%, about 2.0 wt% to about 4.0 wt%, or about 3.0 wt% of a humectant.

Wherein the water-pervious portion comprises from about 0.05 wt% to about 2.0 wt%, from about 0.1 wt% to about 2.0 wt%, from about 1.0 wt% to about 2.0 wt%, from about 1.2 wt% to about 1.8 wt% By weight, or about 1.5% by weight of the polymeric gelling agent. If the amount of the polymeric gellant is less than the above-mentioned content, the effect of stabilization of the nanoemulsion is insignificant because of a small steric hindrance or hydration effect. When the amount of the polymer gelling agent is larger than the above-mentioned content, there is a problem in dissolution and the viscosity is increased, so that the aggregation of the particles can be promoted and the stability can be inhibited.

The average particle size of the nanoemulsion is from about 30 nm to about 150 nm, from about 30 nm to about 140 nm, from about 30 nm to about 130 nm, from about 30 nm to about 120 nm, from about 30 nm to about 110 nm, from about 30 from about 30 nm to about 50 nm, or from about 40 nm to about 50 nm, from about 30 nm to about 90 nm, from about 30 nm to about 80 nm, from about 30 nm to about 70 nm,

The nanoemulsion forms a solid multi-layer lipid membrane having a structure similar to that of cell interleukin by using phospholipids and ceramides, and fermented glycolipids are mixed to improve the skin absorption and application properties of the fermented oil, May be a nanoemulsion having a particle size of 30 to 150 nm which imparts flexibility and contains up to 40% of fermentation oil.

Another aspect includes at least one selected from the group consisting of a lipid thickening portion comprising at least one selected from the group consisting of polyhydric alcohols, phospholipids, ceramide, and glycolipids, an oily portion comprising a fermentation oil, and a moisturizing agent and a polymeric gelling agent Mixing the water phase to form a first concentrated phase;

Hydrating the first concentrated phase by agitating the first concentrated phase to hydrate;

Emulsifying the hydrated first concentrated phase under high pressure to produce a second concentrated phase to obtain a nano emulsion composition; And

And cooling and stabilizing the second thickened phase, to form a nanoemulsion composition according to an aspect.

The above polyhydric alcohols, phospholipids, ceramides, glycolipids, fermentation oils, moisturizers, polymer gelling agents, and nanoemulsions are as described above.

The method comprises the steps of: providing a water-containing portion comprising at least one selected from the group consisting of a lipid thickening portion comprising at least one selected from the group consisting of polyhydric alcohols, phospholipids, ceramides, and glycolipids, an oily portion including a fermentation oil, and a moisturizing agent and a polymer gelling agent And mixing to produce a first concentrated phase.

The method may further comprise preparing a lipid thickening portion by warming at least one selected from the group consisting of polyhydric alcohols, phospholipids, ceramides, and glycolipids. Wherein the lipid enriched portion comprises at least one selected from the group consisting of polyhydric alcohols, phospholipids, ceramides, and glycolipids, at a temperature of about 30 캜 to about 90 캜, about 40 캜 to about 90 캜, about 50 캜 to about 90 캜, About 90 ° C, about 70 ° C to about 90 ° C, about 80 ° C to about 90 ° C, or about 85 ° C.

The method may further include heating the fermented oil to prepare an oily portion. The oily phase can be prepared by warming the fermentation oil at about 30 캜 to about 90 캜, about 40 캜 to about 80 캜, about 50 캜 to about 70 캜, about 60 캜 to about 70 캜, or about 65 캜.

The method may further include a step of warming the mixture of the moisturizing agent and the polymer gelling agent to prepare a water phase. Wherein the water phase comprises a mixture of a humectant and a polymeric gelling agent dissolved in water to a temperature of from about 30 DEG C to about 90 DEG C, from about 40 DEG C to about 80 DEG C, from about 50 DEG C to about 70 DEG C, from about 60 DEG C to about 70 DEG C, Lt; RTI ID = 0.0 > C. ≪ / RTI >

The step of preparing the first concentrated phase may be carried out by mixing simultaneously or sequentially. For example, an oil-in-water portion may be added to a lipid concentration portion, and a water-based portion may be added thereto. The step of preparing the first concentrated phase may be performed while stirring. The stirring may be performed two or more times. The stirring may be carried out using an agitator. The stirring may be performed at about 1,000 rpm to 2,000 rpm, at about 1,100 rpm to 1,900 rpm, at about 1,200 rpm to 1,800 rpm, at about 1,300 rpm to 1,700 rpm, at about 1,400 rpm to 1,600 rpm, or at 1,500 rpm. By stirring, the fermented oil can be bulky and homogenized. The step of preparing the first concentrated phase may be performed while maintaining the temperature at about 50 캜 to about 90 캜, about 60 캜 to about 80 캜, or about 70 캜.

The method includes hydrating a first concentrated phase to hydrate the first concentrated phase.

The step of hydrating the first concentrated phase may be such that the phospholipid mainly composed of the particle wall is sufficiently hydrated and is regularly arranged with the lipids of the lipid enriched portion so as not to have a nonuniform composition due to the concentration gradient of phospholipid and intercellular lipid components have.

The step of hydrating the first concentrated phase may be carried out at a temperature of from about 30 DEG C to about 90 DEG C, from about 40 DEG C to about 80 DEG C, from about 40 DEG C to about 70 DEG C, from about 40 DEG C to about 60 DEG C, . The stirring may be performed for about 1 minute to about 24 hours, for about 10 minutes to about 20 hours, for about 20 minutes to about 16 hours, for about 30 minutes to about 12 hours, for about 30 minutes to about 8 hours, for about 30 minutes to about 4 hours , Or from about 30 minutes to about 1 hour. If the time is less than the above-mentioned time, hydration is not sufficiently carried out. If the time is longer than the above-mentioned time, hydrolysis of phospholipid in water is accelerated and stability may be poor.

The method comprises emulsifying the hydrated first concentrated phase under high pressure to produce a second concentrated phase to obtain a nano emulsion composition.

The step of producing the second concentrated phase has a nano-size of the first concentrated phase that is hydrated, and can efficiently collect a large amount of fermented oil.

The step of preparing the second concentrated phase may be performed at a temperature of from about 40 캜 to about 70 캜, from about 50 캜 to about 60 캜, or about 55 캜. If the temperature is lower than 40 캜, it may be lower than the solidification temperature of the phospholipid, ceramide, or fermented glycolipid, so that it can not be treated in the high-pressure emulsifier. If the temperature exceeds 70 ° C, the fermentation oil itself may be destroyed at a high temperature in addition to the energy applied by the high-pressure emulsifier, or gelation may occur due to high energy and high lipid concentration, so that dense nanoemulsion may not be obtained .

Wherein the step of preparing the second concentrate comprises the steps of: providing a first concentrate having a viscosity of from about 600 bar to about 1,500 bar, from about 700 bar to about 1,500 bar, from about 800 bar to about 1,500 bar, from about 900 bar to about 1,500 bar, From about 1,100 bar to about 1,400 bar, or from about 1,200 bar to about 1,300 bar. When the pressure is less than 600 bar, it is difficult to form nano-sized homogeneous particles. When the pressure exceeds 1,500 bar, the temperature rises sharply due to friction due to too strong force, causing damage to the active fermentation oil, There may be a problem that stability is deteriorated due to dislocation of the back.

The step of producing the second concentrated phase may be performed once, twice, or more using a high-pressure type hydraulic machine.

By the step of producing the second concentrated phase, the fermentation oil can be obtained as a nanoemulsion that is located in the oil phase of the lipid particle, the innermost phase of the multi-phase membrane, or a combination thereof.

The method includes cooling and stabilizing the second concentrate.

The cooling step may cool the second concentrate to about 40 캜 to about 50 캜, about 30 캜 to about 40 캜, or about 40 캜. The cooling may be performed at room temperature. The cooling can be carried out with stirring.

Another aspect provides a cosmetic composition comprising a nanoemulsion according to one aspect.

The cosmetic composition may contain conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and fragrances, thickeners, and carriers in addition to the nanoemulsion according to one embodiment, and may be in any form conventionally produced in the art .

The cosmetic composition may be formulated into a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation and spray. For example, the cosmetic composition may be prepared in the form of a skin, an essence, a lotion, a cream, a foundation, a color cosmetic composition, or a combination thereof.

According to one aspect of the present invention, there is provided a nanoemulsion, a method for producing the same, and a cosmetic composition containing the same, which comprises a fermented oil having excellent skin absorbency and moisturizing ability, enhances metastability when applied to cosmetics, Moisturization can be improved.

1 is a chemical structural formula of phospholipid (A), ceramide (B), and fermented glycolipid (C).
2 is a schematic diagram of particles of a nanoemulsion containing a fermented oil.
Figs. 3A to 3C are the particle size analysis results of the particle sizes of the nanoemulsion of Test Examples 1 to 3, respectively.
4 is an image (A) of the nanoemulsion of Test Example 3 and Comparative Example 6 and an image (B) when the nanoemulsion is dispersed in purified water.
Fig. 5 is an image showing the stability with time of the nanoemulsion of Test Example 3 and Comparative Example 6. Fig.
6 is a graph showing the amount of tocopheryl acetate elution (% by weight) according to the elution time (hour) of Samples 3 and 8.
Fig. 7 is a graph showing the result of skin moisturizing test of lotion with fermented oil applied in Example 3 (AU: arbitrary unit). Fig.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  1. Preparation of nanoemulsion composition of fermented oil and the prepared nanoemulsion of  effect

1. Preparation of Nano Emulsion Composition of Fermentation Oil

(Glycerin, EMERY OLEO), soybean lecithin (SOYA-SPL 75H, Korea, neuropid), ceramide (DS-CERAMIDE, Doosan Biotech, Korea) as a phospholipid, and glycerin The lipid concentrate was mixed with glycolipid (U-Ferment FE, United Active Co., Ltd., Korea) in the contents shown in Table 1 below, and the mixture was heated to 85 캜 for dissolution.

(U-Ferment Oil, United Active) and antioxidant Tocopheryl Acetate (DSM, France) in the contents shown in Table 1 were added to a separate dissolution tank and the mixture was heated to 65 ° C And heated to dissolve.

(INUTEC SL-1, Belgium, Orafti) and Panthenol (France, DSM) as the moisturizing agent were mixed in the contents shown in Table 1 in a separate melting tank And the solution was heated to 65 DEG C to dissolve it.

The water and the oil phase prepared in the dissolution tank containing the lipid concentrate were respectively added and the mixture was firstly emulsified by stirring for 20 minutes at a speed of 1,000 rpm to 2,000 rpm using an agitator while maintaining the temperature at 70 캜 to obtain a fermentation oil Bulkly homogenized first nanoemulsion was prepared. The prepared first nanoemulsion was put into a high pressure emulsifier at 55 ° C and treated twice at a pressure of 1,300 bar to prepare a second nanoemulsion in which fermentation oil was very dense and nano sized. The second nanoemulsion thus prepared was cooled to 40 캜 with a slow agitation using a stirrer to obtain a stabilized nanoemulsion composition of fermented oil.

(Translucency:? (Almost transparent),? (Translucent),? (Translucent to turbid), X (milky white)

The particle size distribution and size of the nano emulsion were analyzed using a particle size analyzer (Nanotracwave, Microtrac Co.), and the results are shown in FIGS. 3A to 3C. As shown in Figs. 3A to 3C, the particle size distribution of Test Examples 1 to 3 was 30 nm to 150 nm, confirming that a nanoemulsion was formed. In addition, good results were obtained in that fermented glycolipids were mixed with a rigid lipid membrane composed of ceramide and phospholipid to form a nano-sized multi-layered nanoemulsion while forming a flexible lipid membrane. The average particle sizes of Test Examples 1 to 3 were measured to be 42 nm, 45 nm, and 43 nm, respectively, and it was confirmed that the nanoemulsion particles were formed into nano-sized particles.

The prepared nanoemulsion composition was observed at 45 캜 for about 3 months (Fig. 4). Test Examples 1 to 3 were confirmed to be very stable with transparency and properties maintained. When the content of the phospholipid was lower than the above-mentioned content as in Comparative Example 1, the particles of the emulsion containing the fermented oil were not formed into nanoscale and lost transparency. When the content of the phospholipid was high as in Comparative Example 2, the viscosity was good until the formation of the second concentrated phase, but the viscosity increased sharply at the stabilization stage of the concentrated phase and the particle size of 10 탆 to 20 탆 And lost transparency. When the content of fermented glycolipids is less than the above-mentioned content as in Comparative Example 3, the fermentation oil does not form a proper nano-size because it has a rigid lipid membrane because it does not give sufficient flexibility to a solid lipid membrane composed of phospholipid and ceramide, And a translucent phase was formed. When the content of the fermented glycolipids was larger than the above-mentioned content as in Comparative Example 4, the lipid membrane was excessively flexible and the fermented oil could not be completely contained on the inner surface, resulting in loss of transparency and formation of a translucent phase. When the content of the polymer gelling agent was less than the above-mentioned content as in Comparative Example 5, the particles were formed to have a good and uniform particle size of about 100 nm. However, when the solution was coated at a room temperature for about 2 months, The particle size was increased due to the high cohesion of the oil and the flexible lipid membrane, and the transparency was lost and finely separated. When the content of the polymer gelling agent is higher than the above-mentioned content as in Comparative Example 6, it has not been possible to form a proper nano-size due to its solubility problem.

2. Nano-emulsion Metastability

In order to confirm that the compositions of Examples 1.1 and Comparative Examples applied to cosmetics had a nano-sized multilayer structure containing metformin and had metastability, 5.0 wt% of each composition was dissolved in 95.0 Add to weight% water and stir. The stability of the prepared mixture, its particle size and stability at 45 캜 for 2 months are shown in Table 2, and the image thereof is shown in Fig. The particle size was measured using a particle size analyzer (Nanotracwave, Microtrac Co.).

Measurement sample Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Initial transparency ◎ ◎ ◎ X X △ △ ○ △ Transparency after 2 months
(45 DEG C) ◎ ◎ ◎ X X X X X X Initial particle size 42 nm 45 nm 43 nm 15.3 탆 11.3 탆 450 nm 435 nm 120 nm 480 nm Particle size after 2 months
(45 DEG C) 50nm 80nm 50nm Phase separation Phase separation Phase separation Phase separation Fine separation Fine separation

Sample 1: Composition of Test Example 1 5.0 wt% + Water 95.0 wt%

Sample 2: Composition of Test Example 2 5.0 wt% + Water 95.0 wt%

Sample 3: Composition of Test Example 3 5.0 wt% + Water 95.0 wt%

Sample 4: Composition of Comparative Example 1 5.0 wt% + Water 95.0 wt%

Sample 5: Composition of Comparative Example 2 5.0 wt% + Water 95.0 wt%

Sample 6: Composition of Comparative Example 3 5.0 wt% + Water 95.0 wt%

Sample 7: Composition of Comparative Example 4 5.0 wt% + Water 95.0 wt%

Sample 8: Composition of Comparative Example 5 5.0 wt% + Water 95.0 wt%

Sample 9: Composition of Comparative Example 6 5.0 wt% + Water 95.0 wt%

Transparency:? (Almost transparent),? (Translucent),? (Translucent to turbid), X (milky white)

As shown in Table 2 and FIG. 5, in order to actually apply the nano emulsion composition to a cosmetic composition in which the continuous phase is a water, the particle size and transparency of the nano emulsion were important as in Test Examples 1 to 3. Also, as in the comparative examples, when the nano-emulsion is turbid immediately after the preparation, the particle size is not formed in the nano-size, and the meta-stability can not be secured even though the particles are formed at the nano- As shown in Table 2, it is difficult to apply the present invention to cosmetics because the fermentation oil is desorbed when dispersed in water and the nano-sized particles stabilizing the fermentation oil can not be obtained.

3. Nano-emulsion  Skin penetration effect

When the formulations of Samples 3 and 8 of Table 2 were applied to the actual skin, the skin penetration effect of the fermented oil was confirmed. However, since the fermentation oil can not measure the percutaneous absorption amount indicating the skin penetration effect, the percutaneous absorption amount of tocopheryl acetate contained together with the day of fermentation was measured in the oily portion.

 The transdermal absorption was determined by adding 0.25 g of sample 3 or 8 to a dissolution tester (ERWE KA DT800 Dissolution Tester, Germany), and using an Enhancer cell equipped with real human skin (65-year-old man's back plate, Ha Ns Biomed) ERWEKA, Germany). This was filled with sodium chloride buffer (about pH 7.0). The amount of tocopheryl acetate eluted was periodically measured by high performance liquid chromatography (HPLC, Japan, shimadzu, LC-10VP) for 12 hours by adjusting the temperature to 37 캜 similar to human body temperature. The results of measuring the elution amount of tocopheryl acetate until about 12 hours are shown in Fig.

As shown in Fig. 6, in the case of Sample 3, the elution amount was about 83.4 with respect to the initial amount introduced, and excellent results were obtained. In the case of Sample 8, the elution amount was about 43.2%, which was similar to the generally obtained elution amount. Therefore, it was confirmed that the nanoemulsion of the test example can emulsify unstable fermentation oil into nano-scale, and when it is applied to various cosmetics, fermented oil can be stabilized and delivered to the skin very effectively.

Example  2. Cosmetics  Preparation of composition

One. Cosmetics  Preparation of composition

Using the nanoemulsion compositions of Test Example 3 in Table 1, skin, essence, lotion, cream, and BB cream compositions having the compositions listed in Tables 3 to 7 below were prepared. As a control, a cosmetic composition was prepared using fermented oil instead of the composition of Test Example 3.

Skin cosmetic composition division ingredient Test group (% by weight) Control (% by weight) Water top Purified water 78.40 84.40 Butylene glycol 10.00 10.00 Carbomer 0.06 0.06 Solubilization portion Solubilizing agent 1.00 1.00 antiseptic 0.50 0.50 Fermented oil - 4.00 Additive part Tromethamine 0.04 0.04 The nanoemulsion of Test Example 3 10.00 -

1) Butylene glycol: Made by DAICEL, Japan

2) Carbomer: LUBRIZOL, USA

3) Solubilizing agent: Polysorbate 20, KAO, Japan

4) Preservatives: Symrise, Germany

5) tromethamine: DOW, USA

Essence cosmetic composition division ingredient Test group (% by weight) Control (% by weight) Water top Purified water 77.22 83.22 glycerin 5.00 5.00 Butylene glycol 5.00 5.00 Carbomer 0.15 0.15 Floating Polyglyceryl-3 methyl glucoside distearate 1.00 1.00 Cetyl alcohol 1.00 1.00 Fermented oil - 4.00 Additive part Tromethamine 0.13 0.13 antiseptic 0.50 0.50 The nanoemulsion of Test Example 3 10.00 -

1) Glycerin: Malaysia, EMERY OLEO

2) Butylene glycol: DAICEL Co., Ltd., Japan

3) Carbomer: LUBRIZOL, USA

4) Polyglyceryl-3 methylglucose distearate: Germany, Evonik

5) cetyl alcohol: Cognis, Germany

6) Fermented oil: United States, United Active

7) Tromethamine: Dow, USA

8) Preservatives: Symrise, Germany

Lotion cosmetic composition division ingredient Test group (% by weight) Control (% by weight) Water top Purified water 73.93 79.93 glycerin 5.00 5.00 Butylene glycol 5.00 5.00 Carbomer 0.20 0.20 Floating Polyglyceryl-3-methylglucoside distearate 1.70 1.70 Cetyl alcohol 1.00 1.00 Dimethicone 0.50 0.50 Shea Butter 0.50 0.50 Caprylic / capric triglyceride 1.50 1.50 Fermented oil - 4.00 Additive part Tromethamine 0.17 0.17 antiseptic 0.50 0.50 The nanoemulsion of Test Example 3 10.00 -

1) Glycerin: Malaysia, EMERY OLEO

2) Butylene glycol: DAICEL Co., Ltd., Japan

3) Carbomer: LUBRIZOL, USA

4) Polyglyceryl-3 methyl glucose diesterate: Germany, Evonik

5) Cetyl alcohol: Cognis, Germany

6) Dimethicone: Shinetsu Co., Ltd., Japan

7) Shea Butter: OLEVA, France

8) Caprylic / capric triglyceride: In the United States, CRODA Inc.

9) Fermented oils: United States, United Active

10) Tromethamine: DOW, USA

11) Preservatives: Symrise, Germany

Cream cosmetic composition division ingredient Test group (% by weight) Control (% by weight) Water top Purified water 63.25 69.25 glycerin 10.00 10.00 Butylene glycol 5.00 5.00 Carbomer 0.30 0.30 Floating Polyglyceryl-3 methyl glucoside distearate 1.70 1.70 Cetyl alcohol 2.50 2.50 Dimethicone 1.00 1.00 Shea Butter 1.50 1.50 Caprylic / capric triglyceride 4.00 4.00 Fermented oil - 4.00 Additive part Tromethamine 0.25 0.25 antiseptic 0.50 0.50 The nanoemulsion of Test Example 3 10.00 -

1) Glycerin: Malaysia, EMERY OLEO

2) Butylene glycol: DAICEL Co., Ltd., Japan

3) Carbomer: LUBRIZOL, USA

4) Polyglyceryl-3 methyl glucose diesterate: Germany, Evonik

5) Cetyl alcohol: Cognis, Germany

6) Dimethicone: Shinetsu, Japan

7) Shea Butter: OLEVA, France

8) Caprylic / capric triglyceride: CRODA, USA

9) Fermented oils: United States, United Active

10) Tromethamine: DOW, USA

11) Preservatives: Symrise, Germany

BB cream cosmetic composition division ingredient Test group (% by weight) Control (% by weight) Floating Dimethicone, trisiloxane 15.00 15.00 Inorganic pigment 0.50 0.50 Piperidine-3-myristyl ether 2.00 2.00 Bis-hydroxyethoxypropyl dimethicone 5.00 5.00 Macadamia seed oil 5.00 5.00 Cyclopentasiloxane / dimethicone crosspolymer 4.00 4.00 Fermented oil - 4.00 Water top antiseptic 0.25 0.25 Butylene glycol 5.00 5.00 Sodium chloride 1.00 1.00 Purified water 52.25 58.25 Additive part The nanoemulsion of Test Example 3 10.00 -

One) Dimethicone, trisiloxane: Dowcorning, USA

2) Inorganic pigments: Korea, Korea synthetic pearl industry

3) PPG-3 myristyl ether: Evonik, Germany

4) Bis-Hydroxyethoxypropyl Dimethicone: Dowcorning, USA,

5) Macadamia seed oil: OLVEA, France

6) cyclopentasiloxane / diemthicone crosspolymer: available from Dowcorning, USA

7) Fermented Oil: United States, United Active

8) Preservatives: Germany, Symrise

9) Butylene glycol: DAICEL Co., Ltd., Japan

10) Sodium chloride: South Korea, one week salt

It was confirmed that the cosmetic composition prepared from the skin, essence, lotion, cream, and BB cream of the nanoemulsion of Test Example 3 remained very stable as a result of 3 months of stability after production. In the case of the control group to which fermented oil was applied directly, stability of the lotion, cream, and BB cream formulations remained stable. However, the skin formulations had difficulty in solubilizing the fermented oil during the production and phase separation occurred. Afterwards, phase separation proceeded.

2. Cosmetics  Of the composition Moisture power

Moisturizing power, i.e., moisture content (A.U .: arbitrary unit) was measured with a corneometer (Courage and Khazaka) for lotion formulations prepared according to Table 5. As a control, a lotion formulation in which a fermented oil was applied instead of the composition of Test Example 3 was used.

Moisture content was measured at 30 min to 8 h before and after application under the condition of constant temperature and humidity (22 ± 2 ℃, relative humidity 50 ± 5%) after washing the arm with foam cleanser. The results are shown in Fig.

As shown in FIG. 7, it was confirmed that the lotion containing the nanoemulsion composition of Test Example 3 was superior in moisture and durability to the control group to which the fermented oil was directly applied.

Thus, the nanoemulsion composition provides a high concentration of fermentation oil at high concentrations, enhances skin penetration and provides moisturizing as well as skin protection when added to foundation and toning cosmetics such as skins, essences, lotions, creams, and BB creams Respectively.

Claims (13)

A nanoemulsion composition comprising a fermentation oil,
21.6 wt.% Polyol containing 20.0 wt.% To 80.0 wt.% Of polyhydric alcohol, 1.0 wt.% To 6.0 wt.% Phospholipid, 0.1 wt.% To 1.0 wt.% Ceramide, and 0.5 wt.% To 5.0 wt. To 92.0% by weight of a lipid thickener;
5.0% to 41.0% by weight oily phase comprising fermentation oil; And
A water-absorbing agent and a polymer gelling agent; and 1.0 wt.% To 42.0 wt.% Of a water phase. The nanoemulsion composition according to claim 1, wherein the fermentation oil comprises at least one selected from the group consisting of argan oil, olive oil, grape seed oil, rice germ oil, sunflower seed oil, and canola oil. . The nanoemulsion composition according to claim 1, wherein the polyhydric alcohol comprises at least one selected from the group consisting of butylene glycol, propylene glycol, glycerin, pentylene glycol, dipropylene glycol, and diglycerin . [4] The method of claim 1, wherein the phospholipid is selected from the group consisting of soybean lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and phosphatic acid Lt; RTI ID = 0.0 > 1, < / RTI > The nanoemulsion composition of claim 1, wherein the ceramide comprises at least one selected from the group consisting of ceramide EOP, ceramide NS, ceramide NP, ceramide AS, and ceramide AP. The nanoemulsion composition according to claim 1, wherein the glycolipid is a fermentation glycolipid comprising at least one member selected from the group consisting of rice germ oil, argan oil, grape seed oil, sunflower seed oil, and canola oil. The method of claim 1, wherein the polymeric gelling agent is selected from the group consisting of inulin lauryl carbamate, polyquaternium-51, mucin, mucopolysaccharide, and biosaccharide gum. < RTI ID = 0.0 > 21. < / RTI > The nanoemulsion composition of claim 1, wherein the fermentation oil is located in the oil phase of the lipid particle, the innermost oil phase of the multiple membrane, or a combination thereof. delete The nanoemulsion composition of claim 1, wherein the oily phase further comprises an antioxidant. 21.6 wt.% Polyol containing 20.0 wt.% To 80.0 wt.% Of polyhydric alcohol, 1.0 wt.% To 6.0 wt.% Phospholipid, 0.1 wt.% To 1.0 wt.% Ceramide, and 0.5 wt.% To 5.0 wt. To 92.0% by weight of a lipid thickener,
From 5.0% to 41.0% by weight of an oily phase comprising fermentation oil, and
A water-absorbing agent and a high molecular weight gelling agent to prepare a first concentrated phase;
Hydrating the first concentrated phase by agitating the first concentrated phase to hydrate;
Emulsifying the hydrated first concentrated phase under high pressure to produce a second concentrated phase to obtain a nano emulsion composition; And
Cooling and stabilizing the second thickened phase,
A method of making the nanoemulsion composition of claim 1. A cosmetic composition comprising the nanoemulsion composition of claim 1. The cosmetic composition according to claim 12, which is a skin, an essence, a lotion, a cream, a foundation, a color cosmetic composition, or a combination thereof.

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