KR101931920B1 - Nano-vesicle comprising Deinococcus extracts, preparation method thereof, and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to a nano-vesicle comprising a Deinococcus extract, a method for manufacturing the same, and a cosmetic composition containing the same. The present invention relates to a nano-vesicle composition which has meta-stability when a carotenoid-containing Deinococcus extract is added to a cosmetic composition, and also effectively transmits through a skin barrier to efficiently deliver active ingredients to the skin.

Description

The present invention relates to a nano bequique containing a deinococcus extract, a process for producing the same, a cosmetic composition containing the deinococcus extract, a preparation method thereof, and a cosmetic composition containing the deinococcus extract,

The present invention relates to a nano bezle comprising a Deinococcus extract, a process for producing the same, and a cosmetic composition containing the same. The present invention relates to a nano beadlet composition which has meta-stability when it is added to a cosmetic composition and efficiently transmits a skin barrier to effectively deliver an effective ingredient to the skin.

Deinococcus sp .; or Micrococcus sp.) Is known to be resistant to ionizing radiation, which can directly damage the DNA or protein of an organism. The tolerance of the Deinococcus strain can be improved by rapidly recovering DNA damage by ionizing radiation through an enzymatic or non-enzymatic system and effectively removing ROS (Reactive Oxygen Species) It is due to ability. Due to these characteristics, there have been a growing number of cases of developing technologies using the Deinococcus strain in various fields such as medicine, healthcare, and biotechnology, but there is no case applied to cosmetics.

Deinococcus extract, which is a substance produced by fermenting a strain of Deinococcus, shows excellent effects on cell regeneration, improvement of skin wrinkles due to antioxidant ability, prevention of aging, etc. However, since it contains oxidation-sensitive carotenoids as some active ingredients, A technique capable of effectively delivering to the skin while blocking the oxidizing environment is needed.

Originally, carotenoids have been shown to exhibit excellent efficacies, such as improving cell wrinkles and antioxidant activity through cell regeneration and antioxidant properties, and have been applied to cosmetics. However, they are easily discolored and deteriorated due to contact with external environment such as air or moisture, Has a problem in that the activity is decreased because of its activity. There have been many attempts to stabilize carotenoids, but they have yet to be effectively applied to commercial products.

Korean Patent Laid-Open No. 10-2005-0095225 ("Cosmetic Composition Comprising Beta-Carotene-Containing Carrot Extract and Method for Producing the Same") filed by Arter Cosmetics Co., Ltd. introduced carrot extract itself containing a small amount of beta carotene into the product Korean Patent No. 10-1833040 (" Cosmetic composition containing physiologically active antioxidants and two-layered cosmetics containing the same ") filed by Daeduk Rapco, a physiologically active antioxidant such as carotenoid is applied to a two- .

However, these conventional methods are limited to small amounts of carnoids administered in the form of extracts from natural form, or to specific formulations such as two-layered formulations, or to use in general encapsulation, etc. These conventional methods involve the use of unstable carotenoid materials Lt; RTI ID = 0.0 > of active < / RTI > cartionic material.

Therefore, there is a need to develop a technique that stabilizes carotenoids to facilitate penetration of the skin, and can be widely used for various formulations.

KR 10-2005-0095225 A1 KR 10-1833040 B1

Disclosure of the Invention An object of the present invention is to provide a nano bequique composition comprising a Deinokokus extract, which has an excellent skin-delivering effect and skin moisturizing effect of an effective ingredient while stabilizing a Deinococcus extract containing a cartinoid.

Another object of the present invention is to provide a method for producing a nanobegicle composition comprising a Deinococcus extract.

It is still another object of the present invention to provide a cosmetic composition comprising a nanobegicle composition comprising a Deinokokus extract.

The present invention has been made to solve the above-mentioned problems, and it has been made to produce nano bezaclo which contains Deinococcus (or Micrococcus ) extract containing a large amount of cartinoids. Specifically, the present invention relates to an aqueous phase comprising Deinococcus extract, Goami extract and a polymeric gelling agent; A lipid concentrate comprising a polyhydric alcohol solvent, a phospholipid, a ceramide, and a rice embryo fermented glycolipid; And an active substance part comprising a Deinococcus extract.

In the nano beadlets of the present invention, Deinococcus extract is collected in a multilayer composed of a phospholipid, a ceramide and a fermented fermented glycolipid of rice embryo, and the vesicle particle wall is hydrated and coated with a polymer gellant by adsorption, (See also the schematic diagram of Fig. 1).

Here, phospholipids and ceramides form solid multi-membrane vesicles, and glycoprotein fermented with rice germ oil, which contains gamma oruzolol and tocopherol and has excellent antioxidant effect, enhances the antioxidant power of bezacel itself and imparts fluidity to the interfacial membrane Thereby facilitating penetration of the nano bezle into the skin. The nanobeads having such a structure can minimize the oxidation of the active ingredients and stabilize them as the Deanokokus extract is contained in the inside of the multilayer membrane.

Furthermore, the Goami extract has high antioxidant ability with high content of vitamin E and phytic acid, and it has an excellent antioxidant ability of the water-soluble part and has a high moisturizing effect through the high amylose composition of the high ami component, and the patch by the amylose polymer network The effect promotes penetration of the active ingredient.

The nano beads of the present invention have high meta-stability when applied to cosmetics, and can promote skin penetration effect and skin moisturizing effect of the active ingredient.

In the present invention, " nano beads " refers to an artificially made vesicle structure of nanometer size. In the present invention, the nano beads may have a multiple-membrane structure. The multi-membrane structure may be a double-layered lipid membrane or lipid bilayer in one nano-bead.

In the present invention, the " Deinococcus extract " is a strain of Deinococcus sp. Having microbial metabolites and capable of rapidly recovering DNA damage by reactive oxygen species (ROS) Micrococcus sp.)). The Deinococcus extract contains carotenoids with excellent efficacy such as skin wrinkles and anti-aging properties by cell regeneration and antioxidant ability. Such carotenoids are easily discolored and detached due to contact with the external environment such as air or moisture. Therefore, it is necessary to study the composition and formulation to prevent contact with the external environment and to maintain the potency. In the present invention, the Deinococcus extract is protected from the external environment through the nano bequecle of the present invention and its excellent antioxidant ability is maintained. Particularly, when applied to skin or the like, the effective ingredient contained in the Deinococcus extract penetrates . In the present invention, Deinococcus sp. And Micrococcus sp. Are mutually compatible (Moseley et al., Jounal of general microbiology, 1983, 129, pp. 2437-2445).

In the present invention, the Deinococcus extract may be contained in the nano beadlets at 10,000 to 60,000 ppm, 10,000 to 40,000 ppm, or especially 20,000 ppm. In addition, in the present invention, the Deinococcus extract may be a substance fermented and / or dissolved from Deinokokus, and may be a substance obtained by filtration and / or purification thereof. Specifically, the Deinococcus extract may be a fermented extract of Deinococcus, a melt, or a filtrate thereof. For example, the Deinococcus fermentation extract filtrate and / or the microcococcus extract Lt; / RTI >

In the present invention, " Goami extract " is an extract of rice (Grain) obtained from GOAMI and contains high contents of amylose, vitamin E, phytic acid and the like. In particular, the present invention can contribute to the formation of a stable form of nano-beadlets having a moisturizing effect by a high amylose content and doubling the penetration power of an active ingredient by a patch effect by an amylose polymer network. Also, it has high antioxidant power by itself because of high content of vitamin E, phytic acid and the like.

In the present invention, " fermented glycolipid " means a complex lipid in which sugars such as glucose and galactose are bonded to lipid moieties and obtained through a fermentation process. The fermented glycolipid in the present invention may include glycolipid fermented with rice germ oil having antioxidant ability, especially containing gamma oryzanol, tocopherol, or the like, or may be mixed with rice germ oil, olive oil, coconut oil, palm oil, grape seed oil , Camellia oil, jojoba oil, camellia oil, sunflower seed oil, apricot seed oil, rice bran oil, rice bran oil, macadamia nut oil, wheat germ oil, safflower seed oil, evening primrose oil, sweet almond oil, avocado oil, hazelnut oil, , Corn oil, rapeseed oil, linseed oil, green tea seed oil, shea butter, cocoa butter oil, mango butter, kukui nut oil, borage oil, carrot seed oil, calendula oil, hemp seed oil, rose hip oil, At least one oil selected from the group consisting of arginine oil. The fermented glycolipid of the present invention can function to increase the antioxidant power of the nano bez be of the present invention and to impart fluidity of the interface membrane. Specifically, the fermented glycolipid of the present invention may include rice embryo fermented glycolipid in which rice germ oil is fermented with a Candida sp. Strain, particularly Candida bombicola microorganism. The fermented glycolipid of the present invention may also be one comprising at least one of glycerol glycolipids, glycolipid and glycerolipids. In the present invention, the fermented glycolipid imparts fluidity to the interfacial membrane, so that the nano beads having a flexible particle wall can pass through the horny layer of the skin to promote the delivery of the effective ingredient including the Deinococcus extract to the skin.

In the present invention, the term " gelling agent " refers to a substance that solidifies a liquid to gel state. In the present invention, the polymer gelling agent is selected from the group consisting of mucopolysaccharide, beta-glucan, mucin, biosaccharide gum, Inulin Lauryl Carbamate, Polyquaternium-51 (polyquaternium-51), and the like. The polymer gellant can be hydrated and coated on the outermost particle wall of the nano bequest of the present invention to stabilize the Deinococcus extract and the like contained in the nano bead, and the nanobegic itself can be stabilized and suitably applied to the cosmetic .

In the present invention, " polyhydric alcohol " refers to an alcohol having two or more hydroxyl groups (-OH) per molecule. Polyhydric alcohols are called divalent alcohols, trihydric alcohols, tetravalent alcohols and the like depending on the number of hydroxyl groups. In the present invention, the polyhydric alcohol may include at least one selected from the group consisting of 1,3-butylene glycol, propylene glycol, glycerin, dipropylene glycol, and methylpropane diol. In the present invention, polyhydric alcohols can increase the efficiency with which lipids are dissolved in water.

In the present invention, " phospholipid " refers to an ester compound of phosphoric acid. In the present invention, the phospholipid may be a glycerol phospholipid or a sphingolipid. Depending on the type of the functional group, a phosphatidylcholine, a phosphatidylethanolamine, a phosphatidylinositol, a phosphatidylserine, Phosphatidylserine). ≪ / RTI >

In the present invention, " ceramide " refers to a compound consisting of sphingosine and a fatty acid, and is also referred to as N-acylsphingosine. The ceramide is a component naturally present in the skin and may contain at least one member selected from the group consisting of ceramide EOP, ceramide NS, ceramide NP, ceramide AS, and ceramide AP as a component that maintains the structure formation and function of the stratum corneum have. In the present invention, ceramide may function to solidify the particle walls of the nano-beadlets by filling in the phospholipids in the particle walls of the nano-beadlets of the present invention.

Specifically, the nanobegique composition of the present invention comprises an aqueous phase comprising from 50 to 85% by weight of a Goami extract, based on the weight of the total composition, and from 0.1 to 5% by weight of a polymeric gelling agent; 0.1 to 5% by weight of phospholipids, 0.1 to 1.5% by weight of ceramide, and 0.1 to 3% by weight of rice embryo fermented glycolipids containing 5 to 25% by weight of hydroxyl group (s) A lipid concentrate; And 5 to 30% by weight of Deinococcus extract.

The present invention also provides a pharmaceutical composition comprising: a) an aqueous phase comprising a goam extract and a polymeric gelling agent; A lipid concentrate comprising a polyhydric alcohol solvent, a phospholipid, a ceramide, and a rice embryo fermented glycolipid; B) adding and stirring the lipid thickening part to the upper part of the water, adding and stirring the active material part to prepare a first concentrated phase, and c) hydrating the primary concentrated phase by stirring at 40 to 70 ° C, and d) treating the hydrated primary concentrated phase with a high-pressure emulsifier to be vasify, To obtain a second concentrated phase to be obtained. The process for preparing the nanobegicle composition of the present invention may further comprise the step of e) cooling and stabilizing the second concentrated phase.

Specifically, the method for preparing a nanobegicle composition of the present invention includes a step of preparing a water phase by dissolving a mixture of a goami extract and a polymer gelling agent. Specifically, it is possible to dissolve Goami extract and polymer gelling agent at room temperature and then heat and dissolve at about 30 to about 90 ° C, or about 50 to about 70 ° C to prepare a water phase, but the present invention is not limited thereto.

In addition, the method for preparing the nanobegicle composition of the present invention includes the step of preparing a lipid thickening part by dissolving at least one selected from the group consisting of a polyhydric alcohol solvent, a phospholipid, a ceramide, and fermented glycolipids (including rice germ fermented glycolipids) do. Specifically, polyhydric alcohol solvents, phospholipids, ceramides, and fermented glycolipids (including rice embryo fermented glycolipids) are heated and dissolved at about 30 to about 90 캜, about 50 to about 90 캜, or about 60 to about 80 캜, But are not limited thereto.

The method for preparing a nanobegicle composition of the present invention comprises preparing a first concentrated phase by mixing a prepared aqueous solution and a lipid thickening part with an active material part containing a Deinococcus extract.

The step of preparing the first concentrated phase may be performed simultaneously or sequentially, and may be performed while stirring. The agitation may be performed using an agitator and may be performed at a range of from about 1,000 to about 2,000 rpm, from about 1,100 to about 1,900 rpm, from about 1,200 to about 1,800 rpm, from about 1,300 to about 1,700 rpm , But is not limited thereto. Through the above stirring, a bulky vesicle in a state in which the Deinococcus extract is homogenized can be formed.

In the method for preparing a nanobegicle composition of the present invention, the prepared first concentrated aqueous phase includes a step of hydration. This hydration step of the first concentration can be carried out through agitation, through which the phospholipids contained in the particle walls are sufficiently hydrated and are regularly arranged with the lipids of the lipid enriched portion to produce a concentration gradient of phospholipids and intercellular lipid components It is possible not to have a non-uniform composition.

The step of hydrating the primary concentrated phase may be performed at a temperature ranging from about 30 ° C to about 90 ° C, from about 40 ° C to about 80 ° C, or from about 40 ° C to about 70 ° C, About 30 minutes to about 12 hours, about 30 minutes to about 8 hours, about 30 minutes to about 4 hours, or about 30 minutes to about 20 hours, about 20 minutes to about 16 hours, about 30 minutes to about 12 hours, But it is not limited thereto. However, if the time is shorter than the above-mentioned time, hydration may not be sufficiently performed, and in many cases, hydrolysis of the phospholipid may occur and the stability may be deteriorated.

The process for preparing the nanobegicle composition of the present invention comprises the steps of emulsifying the hydrated first concentrated aqueous phase prepared above under high pressure to prepare a second concentrated aqueous phase to obtain a nanobedicle. Specifically, the hydrated first concentrated phase may be put into a high-pressure emulsifier, followed by pressure treatment to form a second concentrated phase. The step of preparing the secondary enriched phase may be performed at a temperature of from about 40 캜 to about 70 캜, from about 40 캜 to about 60 캜, or about 50 캜. When the temperature is lower than 40 ° C, the solubilization temperature of the phospholipids and the ceramide components may be lowered, resulting in a problem in the treatment of the high-pressure emulsifier. If the temperature is higher than 70 ° C, The desired physical properties can not be obtained.

Wherein the step of preparing the second enriched phase comprises heating the second concentrate to a temperature of from about 500 bar to about 1,500 bar, from about 600 bar to about 1,500 bar, from about 700 bar to about 1,500 bar, from about 800 bar to about 1,500 bar, From about 1,000 bar to about 1,500 bar, from about 1,100 bar to about 1,400 bar, or from about 1,200 bar to about 1,300 bar. If the pressure is less than 500 bar, it is difficult to form nano-sized homogeneous particles. If the pressure exceeds 1,500 bar, the temperature rises due to the friction due to too strong force, and the active material is destroyed. Problems can arise. The step of preparing such a second concentrated phase may be performed once, twice or more through a high-pressure emulsifier.

The average particle size of the nano beadlets prepared by the method of the present invention is about 30 nm to about 150 nm, about 30 nm to about 140 nm, about 30 nm to about 130 nm, From about 30 nm to about 70 nm, from about 30 nm to about 120 nm, from about 30 nm to about 110 nm, from about 30 nm to about 100 nm, from about 30 nm to about 90 nm, nm to about 50 nm, or from about 30 nm to about 40 nm.

In addition, the nano beadlets prepared by the method of the present invention can be prepared by mixing the aqueous phase and the lipid bilayers, .

The present invention also provides a cosmetic composition comprising the nanobegicle composition of the present invention.

The " cosmetic composition " in the present invention includes the nano bezacique composition of the present invention or the nano bezacique composition prepared by the production method of the present invention. Specifically, the nano bezacique composition of the present invention may be prepared by mixing the cosmetic composition of the present invention with conventional cosmetics for preparing skin, essence, lotion, cream, pack, mask sheet, cleansing, makeup base, foundation, sun cream or lip gloss.

The cosmetic composition of the present invention can be prepared in any form conventionally produced in the relevant industry and can be used in the form of a solution (for example, a skin), a lotion (present solution, an emulsion), an external ointment, a cream, Soap, liquid cleanser, bath, cleansing, sunscreen, sun oil, emulsion, paste, gel, powder, oil, patch, lip balm, lip gloss, makeup base, foundation, essence , And a spray. However, the present invention is not limited thereto. According to one embodiment of the present invention, the cosmetic composition of the present invention can be used as a skin, essence, lotion, cream, pack, mask sheet, cleansing, makeup base, foundation, sun cream or lip gloss Or a mixture thereof with a conventional cosmetic.

In addition, the cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a general skin cosmetic composition, and examples thereof include oil, water, a surfactant, a moisturizer, Auxiliaries, thickeners, chelating agents, pigments, preservatives, flavoring agents, and the like may be appropriately compounded, but are not limited thereto. The cosmetically acceptable carrier to be contained in the cosmetic composition may be variously selected depending on the formulation.

Furthermore, the cosmetic composition of the present invention may be formulated into a functional cosmetic product further comprising a component having an antioxidative effect, a wrinkle improving effect, an anti-aging effect and / or a UV-blocking effect. As the component having the antioxidative effect, the wrinkle improving effect, the anti-aging effect and / or the ultraviolet ray blocking effect, the active ingredient used in the functional cosmetics known in the art can be used without limitation.

The present invention relates to a nano bequique containing a Deinokokus extract, which has an active ingredient such as a Deinokokus extract protected from the external environment to stabilize the active ingredient, and has high metastability when applied to cosmetics and the nano bequecle itself. In addition, when applied to the skin, there was an effect of improving penetration of the active ingredient into the skin and moisturizing the skin.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram of a nano bequique containing a Deinococcus extract of the present invention. FIG. Specifically, the Deinococcus extract was collected inside the multiple membrane composed of the phospholipid, ceramide, and fermented fermented glycolipid of the present invention, and the particle wall was hydrolyzed by adsorbing the polymer gelling agent. It has an antioxidant ability and a skin penetration ability of the active ingredient is enhanced.
2 is a chemical structural formula of Phytoen, which is the main carotenoid produced through the fermentation of Deinococcus sp. Of the present invention.
FIG. 3 is a graph showing the antioxidative effect of the nano beadlets of the present invention through DPPH radical scavenging experiments. As a positive control, vitamin C (0.01% by weight) having antioxidant power was used.
FIG. 4 is a photograph comparing the properties of the nano bequick composition of the present invention (Example 2 of the composition) and the nano-beaded composition of the comparative composition 1 of Table 2 containing adenosine of the present invention.
FIG. 5 is a photograph showing the comparison of the properties of the nano bezacel composition (sample 2) of Table 2 and the nano plastic bead composition of the comparative composition 1 after storing the adenosine of the present invention at 25 ° C for 1 month.
FIG. 6 is a photograph comparing the properties of the nano bezacique composition (composition sample 2) of Table 2 and the nano bezacique compositions of comparative composition samples 4 and 5 containing adenosine of the present invention after storage at 25 ° C for 1 month.
FIG. 7 is a photograph showing the comparison of properties of the nanobegicle composition (sample 2) of Table 2 and the sample 6 of the comparative composition of the present invention containing adenosine of the present invention after storage at 50 ° C for 1 week.
FIG. 8 shows the results of particle size analysis of the nano-beadlets of the composition samples 1 to 3 and the comparative composition samples 1 to 4 and 6 of Table 2 containing adenosine according to the present invention.
FIG. 9 shows a comparative composition sample 1 comprising a nano bead (composition sample 2) according to the present invention and a goamish extract according to the present invention containing Goami extract and containing adenosine in less than the preferred content range of the composition samples 1 to 3. FIG. To 3 of the nano beads, and the cosmetic samples 4 to 6, respectively, in comparison with the results of the percutaneous absorption test of adenosine.
Fig. 10 is a graph showing the results of comparison of results of moisture resistance test when nano beads (composition sample 2) shown in Table 1 according to the present invention is added to cosmetics. Fig.

The present invention will be further clarified through the above-described aspects and the following embodiments. Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  1. Nano containing Deinokokusu extract Vezic  Manufactured and manufactured nano Vezic  Check the effect

One. Nano Vezic  Produce

A nano beadlet obtained by collecting a Deinokokus extract (in particular, a filtrate of a Deinokokkus fermentation extract) in the composition shown in the following Table 1 was prepared.

The lipid concentrate shown in the following Table 1 was put into a separate dissolution tank and heated to 70 ° C to dissolve. In a separate dissolution tank, Was prepared.

The lipid thickening part prepared above was put into a dissolution tank containing the water phase and stirred at a rate of 1,500 rpm for 5 minutes using a stirrer while maintaining the temperature at 50 ° C. The active substance part was added thereto and stirred at a rate of 1,500 rpm for 5 minutes And homogenized by stirring to obtain an excess amount of the Deinococcus extract. Thus, a first concentrated phase was prepared. While maintaining the first concentrated phase at a temperature of 50 캜, the first concentrated phase was sufficiently hydrated by stirring with a stirrer at 500 rpm for 30 minutes. The hydrated first concentrated phase was put into a high-pressure emulsifier at 50 캜 and treated twice at a pressure of 1,300 bar to make a Deinococcus extract as a nano-sized lipid bilayer formed between the innermost aqueous phase and lipid bilayer, And the mixture was cooled to 30 캜 with stirring using a stirrer and stabilized to obtain a nano bead of the Boninekokusu extract.

division ingredient
(weight%) Composition sample The comparative composition sample One 2 3 One 2 3 4 5 6 Water top Purified water - - - 75.40 65.40 35.40 - - - Goami extract 80.90 75.40 53.40 - 10.00 40.00 76.39 71.40 75.40 Mucopolysaccharide 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Lipid concentrate Methyl propanediol 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Phospholipids 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Ceramide 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Rice embryo fermented glycolipids 0.50 1.00 3.00 1.00 1.00 1.00 0.01 5.00 - Glycollipride - - - - - - - - 1.00 Active material part Deinokokusu fermentation extract filtrate 5.00 10.00 30.00 10.00 10.00 10.00 10.00 10.00 10.00 Properties pH 6.00 5.80 6.00 6.20 5.90 6.00 6.20 6.00 6.10 transparency ◎ ◎ ○ ○ ○ ○ ○ X ○

* Transparency: ◎ (almost transparent), ○ (translucent), △ (translucent-turbid), X (milky white).

The components used for preparing the nanobegicle composition are as follows.

- Goami extract: Goami extract, Korea, NB BIO.

- myuko polysaccharide: U-active ^® Real vera, Republic of Korea, United activated.

- Methylpropanediol: DUB DIOL, France, Srearinerie Dubois.

- Phospholipids: SOYA-SPL 75H, Korea, NEUROPID.

- Ceramide: DS-CERAMIDE, Korea, Doosan Biotech.

- fermented rice germ glycolipid: U-Ferment ^® FE, Republic of Korea, United activated.

- Glycolipid: MELavo, South Korea, Decay Bio.

- Deinokokusu fermentation extract filtrate: BIOXINOID (200,000 PPM), Korea, LABIO.

2. Nano for measuring skin penetration effect Vezic  Produce

In order to measure the penetration effect of the Deinokokkus extract, 0.40% by weight of adenosine was contained in the active substance portion, because the amount of elution indicating the skin penetration effect of the Deinococcus extract was difficult to be directly measured. The nanobegicle composition prepared is shown in Table 2 below.

division ingredient
(weight%) Composition sample The comparative composition sample One 2 3 One 2 3 4 5 6 Water top Purified water - - - 75.00 65.00 35.00 - - - Goami extract 80.50 75.00 53.00 - 10.00 40.00 75.99 71.00 75.00 Mucopolysaccharide 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Lipid concentrate Methyl propanediol 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Phospholipids 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Ceramide 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Rice embryo fermented glycolipids 0.50 1.00 3.00 1.00 1.00 1.00 0.01 5.00 - Glycollipride - - - - - - - - 1.00 Active material part Deinokokusu fermentation extract filtrate 5.00 10.00 30.00 10.00 10.00 10.00 10.00 10.00 10.00 Adenosine 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 Properties pH 6.00 5.80 6.00 6.20 5.90 6.00 6.20 6.00 6.10 transparency ◎ ◎ ○ ○ ○ ○ ○ X ○

The ingredients used in the preparation of the nanobegicle composition of Table 2 above are as described for the ingredients used in Table 1. Adenosine which is further used is as follows.

Adenosine: ADENOSINE, Japan, ZMC.

3. Nano Vezic  Measurement of particle size and physical / chemical stability effect

The particle size of the nano beads was analyzed using a particle size analyzer. The size of the nanobeads is preferably from 30 to 150 nm. In this experiment, particle size was observed using a particle size analyzer (Microtrac Nanotracwave, USA).

In the case of the above composition samples 1 to 3, the Deinococcus extract was concentrated to 30 to 150 nm to obtain a stable form of the vesicle particles. As a result of analysis by a particle size analyzer, the average particle size was 70, 80, It was confirmed that nanoparticles were well formed at 120 nm. In addition, it was observed at 50 캜 for about one month, and it was confirmed that it was stable with keeping relatively transparency and properties.

On the other hand, in the comparative composition samples 1 to 3, the content of goamishi extract was smaller than that of the composition samples 1 to 3. In this case, there was no significant difference in the initial transparency, but it became opaque after one month at room temperature, It was discolored with light. The comparative composition sample 4 had a rice embryo fermented glycolipid content of less than the preferred content range of the composition samples 1 to 3. In this case, the nano size of about 100 nm was formed with good transparency, It became opaque afterwards and observed discoloration with yellowish color. Also, the comparative composition sample 5 had a content of rice germ fermented glycolipids in an amount exceeding a desirable range, and in this case, it was opaque and had a problem of increasing viscosity and phase separation. In addition, the comparative composition sample 6 contained glycollipide instead of the fermented glycoglyceride of rice embryo. In this case, the initial transparency was good, but after 1 month at room temperature, the liquid state was maintained and became opaque. After one week at 50 ° C, There was a problem of gelation showing an opaque characteristic.

4. Nano Vezic  Antioxidant effect measurement

The antioxidative effect of the composition 2 Nano Vezic composition of Table 1 was measured by the DPPH radical scavenging effect. Vitamin C, a potent antioxidant, was used as a positive control, and nanoparticles containing composition 100%, 50%, 25%, and 10% And the DPPH radical scavenging effect was measured. The results are shown in Table 3 (FIG. 3).

Treating material Vitamin C Nano Vezic Concentration (% by weight) 0.01 100 50 25 10 DPPH radical removal effect 85 45 24 10 4

Experimental results shown in Table 3 show that the composition containing the nanobegicle composition of the present invention has an antioxidative effect that increases in a concentration-dependent manner.

5. Nano Vezic Metastability and  Measure skin penetration effect

In order to confirm the applicability of the nano bezze composition of the present invention to cosmetics, metastability and skin penetration test were conducted.

First, 10.0% by weight of each of the composition sample and the comparative composition sample of Table 2 were added to 90.0% by weight of water, and the cosmetic samples 1 to 9 were prepared by simple stirring. The results are summarized in Table 4 below.

Measurement sample Cosmetic sample 1 Cosmetic sample 2 Cosmetic sample 3 Cosmetic sample 4 Cosmetic sample 5 Cosmetic sample 6 Cosmetic sample 7 Cosmetic sample 8 Cosmetic sample 9 Particle size 70 nm 82 nm 117 nm 150 nm 156 nm 153 nm 100 nm 120 um 60 nm transparency ◎ ◎ ○ ○ ○ ○ ○ X ○ After one month, the particle size (50 ° C) 78 nm 80 nm 110 nm 120 um 600 um 450 nm 600 nm Phase separation Phase separation Transparency after 1 month (50 ℃) ◎ ◎ ○ X X △ △ X X

* Cosmetic sample 1: Composition of composition sample 1 10.0% by weight + water 90.0% by weight;

Cosmetic sample 2: composition of composition sample 2 10.0% by weight + water 90.0% by weight;

Cosmetic sample 3: composition of composition sample 3 10.0% by weight + water 90.0% by weight;

Cosmetic sample 4: Comparative composition 10.0% by weight of the composition of sample 1 + 90.0% by weight of water;

Cosmetic sample 5: Comparative composition Composition of sample 2 10.0% by weight + water 90.0% by weight;

Cosmetic sample 6: Comparative composition Composition of sample 3 10.0% by weight + water 90.0% by weight;

Cosmetic sample 7: comparative composition 10.0% by weight of the composition of sample 4 + 90.0% by weight of water;

Cosmetic sample 8: comparative composition 10.0% by weight of sample 5 + 90.0% by weight of water;

Cosmetic sample 9: comparative composition 10.0% by weight of sample 6 + 90.0% by weight of water.

As shown in the results of Table 4 and FIGS. 4 to 7, the composition samples 1 to 3 having the preferable composition of the present invention are excellent in meta-stability and applicable to cosmetics. However, in the case of comparative composition samples, And stability problems such as opacity or separation of the formulations themselves were observed as shown in Table 4 because the meta-stability was not ensured. These comparative composition samples are expected to be practically difficult to apply to cosmetics.

Skin penetration effects of the cosmetic samples 2 (composition samples 2) and cosmetic samples 4 to 6 (comparative compositions 1 to 3) in Table 4 were measured, and the results are shown in FIG.

The transdermal absorption was carried out in an Enhancer cell (ERWEKA, Germany) equipped with the same amount of a sample of cosmetic sample 2 and cosmetic sample 4 to 6 in a dissolution tester (ERQEKA DT800 Dissolution Tester, Germany) The elution amount of adenosine was periodically measured by high-performance liquid chromatography (HPLC, Japan, Shimadzu, LC-1, Japan) by filling sodium chloride buffer (about pH 7.0) and adjusting the temperature to 37 캜 similar to the actual human body temperature. 10VP). The result of measuring the amount of adenosine elution after about 12 hours is shown in FIG.

9, the elution curve of adenosine to be delivered showed excellent results when the amount of elution was about 96.8% with respect to the initial amount (0.04% by weight) of cosmetic sample 2, In the case of Sample 4, Cosmetic Sample 5 and Cosmetic Sample 6, the elution amounts were about 70.8%, 75.3% and 81.7%, respectively, but the efficiency was lower than that of the cosmetic sample 2 mentioned above.

This means that as shown in FIG. 9, effective penetration of the goami extract by the amylose network has doubled the active ingredient delivery.

Example  2. Nano containing Deinokokusu extract Vezic  Manufacture of various cosmetics and their effects

1. Preparation of cosmetic composition

In Table 1 of Example 1, a skin, essence, lotion, cream, and foundation cosmetic having the compositions shown in Tables 5 to 9 were prepared (application example) using the nano-beaded composition of the composition sample 2, Unused cosmetic compositions were prepared as comparative examples.

On the other hand, the components used in the following essence, lotion, cream and foundation cosmetic composition compositions are specifically as follows.

- Methylpropanediol: DUB DIOL, France, Srearinerie Dubois.

- 1,2-hexanediol: HYDROLITE 6 O, Singapore, SYMMRISE.

- Ethanol: Ethanol, Republic of Korea, purified water.

-Page-40 Hydrogenated castor oil: Cremophor RH40, BASF, Germany.

- Deinokokusu fermented extract: BIOXINOID (200,000 PPM), Korea, LABIO.

- Sodium hyaluronate: HYALURONATE, Japan, TSUTO.

- Carbomer: CARBOPOL 980, USA, LUBRIZOL.

- Tromethamine: TRIS AMINO ULTRA PC, USA, DOWCHEMICAL.

-Dihydro jeneyi lactide poly (C ₆ - ₁₄ olefin): PURESYN 4, United States, EXXONMOBILCHEMICAL.

- Jojoba seed oil: JOJOBA OIL, USA, MCY.

- polyglyceryl-3 methyl glucose diesterate: TEGO CARE 450, Germany, EVONIK.

- Cetearyl alcohol: LANETTE O, Germany, BASF.

- Ozokerite: OZOKERITE WAX 77W, USA, ROSS.

- sorbitan olivate: OLIVEM 900, USA, HALLSTAR.

- Bis-Phage / Pefji-14/14 Dimethicone: ABIL EM 97, Germany, EVONIK.

-Ethylhexylmethoxycinnamate: PARSOL MCX, The Netherlands, DSM.

- phenyl trimethicone: DC 556, USA, DOW CORNING.

-Cyclopentasiloxane, cyclohexasiloxane: DC 345, USA, DOW CORNING.

- Sodium chloride: Salt, USA, Cargill.

Composition of skin cosmetic composition division Component (% by weight) weight% Comparative Example Application example Water top Purified water 88.50 79.50 Methyl propanediol 5.00 5.00 1,2-hexanediol 2.00 2.00 Solubilization portion ethanol 3.00 3.00 FAGE-40 Hydrogenated
Castor oil 0.50 0.50 Additive part Deinokokusu fermentation extract filtrate 1.00 - Composition Sample 2 - 10.00

Composition of essence cosmetic composition division Component (% by weight) weight% Comparative Example Application example Water top Purified water 88.00 79.00 Methyl propanediol 5.00 5.00 Sodium hyaluronate 0.10 0.10 1,2-hexanediol 2.00 2.00 Increasing age Carbomer 0.20 0.20 Solubilization portion Methyl propanediol 3.00 3.00 FAGE-40 Hydrogenated
Castor oil 0.50 0.50 Neutralizing agent Tromethamine 0.20 0.20 Additive part Deinokokusu fermentation extract filtrate 1.00 - Composition Sample 2 - 10.00

Composition of lotion cosmetic composition division Component (% by weight) weight% Comparative Example Application example Water top Purified water 83.55 74.55 Methyl propanediol 5.00 5.00 Sodium hyaluronate 0.05 0.05 1,2-hexanediol 2.00 2.00 Increasing age Carbomer 0.20 0.20 Floating Dihydro jeneyi lactide poly (C ₆ - ₁₄ olefin) 5.00 5.00 Jojoba seed oil 1.00 1.00 Polyglyceryl-3 methyl glucoside distearate 2.00 2.00 Neutralizing agent Tromethamine 0.20 0.20 Additive part Deinokokusu fermentation extract filtrate 1.00 - Composition Sample 2 - 10.00

Composition of cream cosmetic composition division Component (% by weight) weight% Comparative Example Application example Water top Purified water 75.05 66.05 Methyl propanediol 5.00 5.00 Sodium hyaluronate 0.05 0.05 1,2-hexanediol 2.00 2.00 Increasing age Carbomer 0.20 0.20 Floating Dihydro jeneyi lactide poly (C ₆ - ₁₄ olefin) 10.00 10.00 Jojoba seed oil 2.00 2.00 Polyglyceryl-3 methyl glucoside distearate 2.50 2.50 Cetearyl alcohol 2.00 2.00 Neutralizing agent Tromethamine 0.20 0.20 Additive part Deinokokusu fermentation extract filtrate 1.00 - Composition Sample 2 - 10.00

Composition of foundation cosmetic composition division Component (% by weight) weight% Comparative Example Application example Floating Ozokerite 1.00 1.00 Sorbitan olivate 1.00 1.00 Bis-phage / phage-14/14 dimethicone cyclopentasiloxane 4.00 4.00 To a solution of < RTI ID = 0.0 & 4.00 4.00 Phenyl trimethicone 2.00 2.00 Cyclopentasiloxane, cyclohexasiloxane 36.00 36.00 Inorganic pigment 0.50 0.50 Water top Purified water 38.50 29.50 Methyl propanediol 8.00 8.00 Sodium chloride 2.00 2.00 1,2-hexanediol 2.00 2.00 Deinokokusu fermentation extract filtrate 1.00 - Composition Sample 2 - 10.00

Tables 5 to 9 are examples of application of the nano bezze composition corresponding to sample 2 of the present invention to real skin essence, lotion, cream, and foundation cosmetic.

As a result of observing the stability of the composition (application example) and composition (comparative example) to which the composition sample 2 of the appropriate concentration was applied in each formulation at the appropriate concentration, the comparative example and application example were not different from each other in the initial physical properties and properties We confirmed the sound, but confirmed that there was a difference thereafter. Specifically, in the case of the comparative example in Table 5, after one week in the fluorescence condition, the turbidity was increased and discolored into yellow light. In the comparative example of Tables 6 to 9, the viscosity was suddenly dropped or discolored after 2 weeks, Respectively. In other words, if the Deinococcus extract is directly applied to cosmetic formulations, there is no great difficulty in initial formulation, but the physical properties of the formulation and discoloration may occur in a short period of time, resulting in a problem of stability.

2. Measurement of moisturizing power of cosmetic composition

Comparative Example Prepared According to Table 8 and Application Cream The cosmetic composition was measured for moisture with a moisture meter (Corneometer, Courage and Khazaka). Specifically, arms were washed with a foam cleanser, and after 30 minutes, 2 hours, 3 hours, 4 hours, 6 hours, 8 hours The moisture content of the arm skin was measured. The results are summarized in Fig.

As can be seen from FIG. 10, it can be seen that the application example cream to which the nano bezacel composition of the present invention is applied has superior moisture and durability than the comparative example cream in which the Deinococcus extract is applied as it is. This is because the phospholipid, ceramide and rice embryo fermented glycolipids that form the particle wall are absorbed by the skin to improve the skin barrier function as well as skin moisturizing effect and the high amylose content of the goami extract improves the skin moisturizing power by forming a skin protective film see.

In summary, as described above, the nanobegic composition according to the present invention stabilizes the Deinococcus extract to a maximum of 60,000 ppm and applies it to foundation and color cosmetics such as skin, essence, lotion, cream, foundation, It was confirmed that the fermented extract of Kus was effectively delivered to the skin.

Although the technical idea of the present invention has been described above with reference to some embodiments and examples shown in the accompanying drawings, it should be understood that the technical scope of the present invention is not limited to the scope of the present invention, It will be understood that various substitutions, changes and modifications may be made without departing from the spirit and scope of the invention. It is also to be understood that such substitutions, modifications and variations are intended to be included within the scope of the appended claims.

Claims (14)

An aqueous phase comprising from 50 to 85% by weight of goamish extract, based on the weight of the total composition, and from 0.1 to 5% by weight of a polymeric gelling agent; 0.1 to 5% by weight of phospholipids, 0.1 to 1.5% by weight of ceramide, and 0.1 to 3% by weight of rice embryo fermented glycolipids containing 5 to 25% by weight of hydroxyl group (s) A lipid concentrate; And 5 to 30% by weight of a Deinococcus extract. The method according to claim 1,
Wherein the Deinococcus extract is a Deinococcus fermentation extract, a lysate, or a filtrate thereof. The method according to claim 1,
Wherein the Deinococcus extract is a substance produced by fermentation of Deinococcus sp. And has a content of 10,000 ppm to 60,000 ppm in the nano bead. The method according to claim 1,
Wherein the fermented glycolipid is selected from the group consisting of rice germ oil; and olive oil, coconut oil, palm oil, grape seed oil, castor oil, jojoba oil, camellia oil, sunflower seed oil, apricot seed oil, rice bran oil, rice bran oil, macadamia nut oil, wheat Soybean oil, corn oil, rapeseed oil, green tea seed oil, shea butter, cocoa butter oil, mango butter, kukui nut oil, barley oil, corn oil, soybean oil, ginger oil, safflower seed oil, sweet almond oil, avocado oil, Wherein the mixture is obtained by fermenting a mixture of at least one oil selected from the group consisting of oil, carrot seed oil, calendula oil, hemp seed oil, rose hip oil, nematic oil, and argan oil. The method according to claim 1,
Wherein the fermented glycolipid comprises at least one of glycerol glycolipids, glycolipid, and glycerolipids. The method according to claim 1,
Characterized in that the polymeric gellant comprises at least one selected from the group consisting of mucopolysaccharide, beta-glucan, mucin, biosaccharide gum, Inulinlauryl carbamate, and polyquaternium-51 Nano bezacel composition. The method according to claim 1,
Wherein the polyhydric alcohol comprises at least one member selected from the group consisting of 1,3-butyleneglycol, propylene glycol, glycerin, dipropylene glycol, and methylpropanediol. The method according to claim 1,
Wherein the phospholipid is at least one selected from the group consisting of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylserine. The method according to claim 1,
Wherein the ceramide is at least one selected from the group consisting of ceramide EOP, ceramide NS, ceramide NP, ceramide AS, and ceramide AP. a) 50 to 85% by weight of goamish extract and 0.1 to 5% by weight of polymeric gelling agent based on the weight of the whole composition are dissolved at room temperature and heated to 50 to 70 캜 to dissolve the mixture to prepare a water phase; 0.1 to 5 wt% of a phospholipid, 0.1 to 1.5 wt% of a ceramide, and 0.1 to 3 wt% of a rice embryo fermented glycolipid having at least two hydroxyl groups of 5 to 25 wt% Lt; RTI ID = 0.0 > 80 C < / RTI > to prepare a lipid concentrate; Preparing an active material portion containing 5 to 30% by weight of Deinococcus extract,
b) adding and stirring the lipid concentrate to the upper portion of the water, adding and stirring the active material portion to produce a first concentrated phase, wherein the stirring is performed at a rotation speed of 1,000 to 2,000 rpm;
c) stirring the first concentrated phase at 40 to 70 DEG C for 0.5 to 1 hour to hydrate,
d) The hydrated first concentrated aqueous phase is put into a high-pressure emulsifier at 40 to 70 ° C, treated with a pressure of 500 to 1,500 bar once or twice to be vigorized to obtain a second concentrated aqueous phase, Producing; And
e) cooling said second concentrated phase to between 30 and 40 < 0 > C to stabilize said nanobedicle composition. 11. The method of claim 10,
Wherein the nano bead preparation comprises a water phase formed between the innermost aqueous phase and the lipid bilayer, and the aqueous phase contains the Deinococcus extract. 11. The method of claim 10,
Wherein the Deinococcus extract is a Deinococcus fermentation extract, a lysate, or a filtrate thereof. A nano beadlet composition prepared by the method of claim 10. 14. A cosmetic or dermatological composition comprising the nanobegicle composition according to any one of claims 1 to 9 and 13, wherein the composition comprises a skin, essence, lotion, cream, pack, mask sheet, cleansing, makeup base, foundation, Cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics.

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