KR101834060B1

KR101834060B1 - Cosmetic Composition And Manufacturing Method Of Cosmetic Composition And Manufacturing Method Of Cosmetic Using Cosmetic Composition

Info

Publication number: KR101834060B1
Application number: KR1020160015098A
Authority: KR
Inventors: 임재영
Original assignee: 임재영
Priority date: 2016-02-05
Filing date: 2016-02-05
Publication date: 2018-03-02
Also published as: KR20170093551A

Abstract

The present invention relates to a cosmetic composition, a method of manufacturing a cosmetic composition, and a method of manufacturing a cosmetic composition using the cosmetic composition, which are produced so as to produce a cosmetic fermented with a fermented persimmon juice, To thereby produce an extract; And fermenting the Geruk extract to produce a fermentation broth.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition,

TECHNICAL FIELD The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, To a method for manufacturing cosmetics using the same.

A potent mechanism for skin aging is known as oxidation of the body or 'oxidative stress' due to the generation of free radicals. These free radicals are made from almost all molecules when they lose electrons and are produced in the mitochondria during cellular respiration when free energy binds to stable oxygen. Cells have enzymes that convert oxygen to reactive oxygen species (ROS), superoxide (O2 ??) or hydrogen peroxide (H2O2), but their action is significantly lower than that of the mitochondrial respiratory chain.

This person's mitochondria accepts 400 liters of O2 a day and converts to water, which is a four-electron reduction process. If only a portion of this O2 is produced in a single electron-reduction pathway, then the amount of ROS exceeds that of any other mechanism. These ROS rapidly associate with other cell compounds and damage such as mitochondria and proteins.

Antioxidants act to neutralize free radicals and to neutralize free radicals.

The study of the free radical theory on aging became more active in 1956 by superoxide dismutase (SOD). SOD can change the superoxide anion to oxygen (O2) and H2O2 (hydrogen peroxide) by throwing or receiving electrons from manganese (Mn) or copper (Cu), which is also catalase It is believed that it is possible to extend the lifespan depending on the SOD enzyme activity ratio and gene expression. Over time, oxidative damage persists in cells and tissues, causing many changes in the body that occur as a result of aging. Free radicals are not only associated with aging but also with degenerative diseases such as cancer, atherosclerosis, and cataracts.

Free radicals may be produced by external causes of skin aging, such as smoking or UV damage. In this case, the skin's own antioxidant network, such as that of vitamin E11 and C, protects against damage by helping to destroy free radicals. However, because of the antioxidants that are not enough to overcome the free radical generation, free radicals do not damage the cell defense mechanisms and damage the skin, but the skin has a repair mechanism for such damage. However, as the time elapses, the repair mechanism gradually weakens, leading to apoptosis and apoptosis, resulting in skin aging. In addition, ROS causes adverse effects on cells. Because ROS oxidizes unsaturated fats in the fluid surrounding the cells (lipid peroxidation), the fluid membrane does not adequately protect the cells, and because nutrients can not pass through the cells, waste accumulates and cells can not function efficiently . In addition, some types of oxidized fats are converted into aldehydes (ie, toxic chemicals) that enter the cells, damaging them, causing an inflammatory reaction, and eventually causing apoptosis that kills younger, . In addition, ROS increases the collagenase, a protein enzyme that breaks down the supporting tissues of the skin, reducing the firmness of the skin, directly damaging the DNA of the cell, Thymine dimer 'is formed, and this small chemical change leads to the irreversible conjugation of DNA molecules, making them no longer able to provide the information necessary to make essential proteins of cells or to make healthy new cells.

Korean Patent No. 10-0566933 (registered on Mar. 23, 2006) discloses a method of manufacturing a herbal cosmetic product and a cosmetic product thereof, which comprises the steps of: , Dried pomegranate, peony, white mulberry, mint, mugwort, seaweed (horse outposts or kelp), and juniper's leaves and berries in natural drying for more than one month in semi-hatchlings, and plums, yulmu, For 10 days ~ 15 days for mulberry, 5 ~ 10 days for mulberry, 30 days for mugwort, 10 ~ 15 days for mulberry, angelica, licorice, , 0.75 wt% of plums, 0.75 wt% of plum, 0.75 wt% of omija, 0.75 wt% of acorn, 0.75 wt% of alum, 0.75% by weight, 0.5% by weight of wheat bran, 0.5% by weight of white paper, 0.5% by weight of hard paper, 0.5 weight% of citron, 0.5 weight% of licorice, 0.5 weight% of peony, 0.5 weight% of peony, 0.5 weight% of Saururus, 0.5 weight% , 1.5% by weight of leaves of juniper and 1.5% by weight of fruit, and heating and distilling from the heated mixture. According to the disclosed technology, not only is it excellent in moisturizing effect, it also activates skin's immune cells due to effective ingredients of herbal medicines and inhibits skin diseases that can come from various external antigens affecting antibody formation of the immune system, Functional cosmetics having an effect that can be beneficial to the herbal composition can be obtained.

Korean Registered Patent No. 10-1223682 (registered on Jan. 11, 2013) appropriately mixes materials containing various kinds of effective ingredients so as to reduce unnecessary things such as discomfort and time wasting to use different kinds of different functional items separately, (Container), and a cosmetic composition for the functional cosmetic composition. According to the disclosed technique, kaolin, germanium, lime and loess are mixed in a 1/4 volume ratio, respectively, and kneaded with clean water to produce a syrup, syrup, pseudo-acacia, 400 ~ 800g each of ginseng, lotus root, mulberry bark, mulberry, ginseng, ginger and licorice are put into a bag, and 200 ~ 400g of camellia fruit, camellia fruit, sunflower fruit, 200 ~ 400g each of mint, chrysanthemum, chrysanthemum, thyme, sandalwood and papaya, put 500 ~ 1,000g of malt sugar and 250 ~ 500g of brown sugar and inject 6 ~ 12ℓ of hot spring water or distilled water containing 4-8 liters and minerals. Then, the container and the bottom were coated with a dough mixed with the same mixture as that used for making the container to a thickness of 15 cm, and the container containing the materials was transferred The first step of fermenting the materials for 3 to 6 weeks by heating the inside temperature of the yellowing oval to 30 to 60 ° C and then filtering the juice to obtain an extract; and a step of separately drying 96 to 98% by weight of the extract 200 ~ 400g of bamboo shoots, 150 ~ 300g of white bamboo shoots, white algae, white pearl beads and white algae respectively and 150 ~ 300g of bamboo salt and boric acid, respectively. Crushing and fine powdering; 0.375 to 0.75 L of camellia oil and 0.1875 to 0.375 L of castor oil, sunflower oil and soybean oil respectively into a fine powder material; mixing the mixture 2 to 4 % Of the extract (96 to 98% by weight) with the above extract.

The conventional functional cosmetics as described above are formed by preparing a mixture having merits such as skin whitening, ultraviolet screening, moisturizing, etc., and have little effect on prevention of aging of the skin desired by the consumer. Therefore, It has disadvantages. In other words, conventional functional cosmetics have a disadvantage in that it is difficult to prevent the fundamental cause of skin aging because the active oxygen-removing substance, which is a fundamental cause of skin aging, is not mixed.

Korean Patent No. 10-0566933 Korean Patent No. 10-1223682

The object of the present invention is to provide a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, And a method of manufacturing cosmetics using the cosmetic composition.

As a means for solving the above-mentioned problems, according to one aspect of the present invention, there is provided a process for producing an extract, comprising the steps of: And fermenting the Geruk extract to produce a fermentation broth.

In one embodiment, the process for producing a goethite extract comprises: a pulverizing step of washing the goethite with purified water, followed by drying and pulverizing; Reflux extraction step in which ethanol is added to the crushed gecko and reflux extraction is carried out; And a step of producing the crude extract by filtering and drying the refluxed goethite, followed by drying.

In one embodiment, the process for producing the gecko fermentation broth comprises: a first gecko broth solution production step of adding glucose to the gecko extract, inoculating the fermented mung bean culture broth, and fermenting the fermented mushroom culture broth to produce a first gecko broth; Producing a second lean culture medium by adding glucose to the first lean culture medium and then inoculating and culturing the culture medium of the mushroom strain to produce a second lean culture; And adding the ethanol to the second nutrient culture solution, filtering and drying the nutrient solution to produce a fermentation broth.

In one embodiment, the first lean culture solution producing step is characterized in that the Leucocephaly extract is fermented at a predetermined pH and maintained at a predetermined temperature for a preset period of time.

In one embodiment, in the first step of producing the gradually growing medium, the fermented Geruk extract is centrifuged and sterilized to produce the first step growth medium.

In one embodiment, the second lean media is produced by fermenting the first lean media at a predetermined pH at a predetermined temperature for a predetermined period of time.

In one embodiment, ethanol is added to the second geckotrophic culture solution, followed by cold-filtration after reflux extraction for a predetermined number of times, followed by filtration.

In one embodiment, the step of producing the gecko fermentation broth is characterized in that the Gecko fermentation broth is concentrated under reduced pressure and freeze-dried.

As a means for solving the above-mentioned problems, according to one aspect of the present invention, there is provided a method for producing a fermentation broth comprising a crude fermentation broth, a platinum colloid aqueous solution, a purified water, glycerin, a phygidecetaldehyde condensate / vinyl dimethicone crosspolymer, a cyclopentasiloxane, , Water extract, sodium chloride, phenoxyethanol, phage-10 dimethicone, propolis extract, fragrance extract, horseradish extract, allantoin, prickly pear fruit extract, disodium edithia, benzophenone-5, The present invention provides a cosmetic composition comprising a fruit extract, a dipropylene glycol, a squalane extract, a licorice extract, a licorice extract, an acanthopanax extract, a broiler extract, a bamboo leaf extract, saponin and a green tea extract.

As a means for solving the above-mentioned problems, according to one aspect of the present invention, there is provided a cosmetic composition comprising: a softening material, a nutritional cosmetic, an essence, a nutritional cream, a pack, a lotion, a gel, a water-soluble liquid, a cream, an emulsion, The present invention provides a method for manufacturing a cosmetic using a cosmetic composition comprising one selected from the group consisting of a skin cover, a lipstick, and a face powder, mixing a cosmetic composition with the selected one, and formulating one with a cosmetic composition.

According to the present invention, there is provided a cosmetic composition, a method of manufacturing a cosmetic composition, and a method of manufacturing a cosmetic composition using the cosmetic composition so as to produce a fermentation broth by fermenting Gecko and producing a cosmetic product to which a Gecko fermentation broth is added, Lycopene, beta-carotene and other antioxidants to effectively prevent the aging of human cells and organs. In other words, it is possible to extract lycopene and beta-carotene from Kaeuk by mixing cosmetic composition by mixing Kaeck fermentation broth and platinum colloid, and the extracted lycopene and beta carotene exert a greater synergy effect on the removal of active oxygen of platinum, It has an effect of preventing the root cause.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart for explaining a method for producing a cosmetic composition according to an embodiment of the present invention. FIG.
FIG. 2 is a flow chart illustrating the process of generating the Geruk extract in FIG. 1; FIG.
FIG. 3 is a flow chart illustrating the process of producing the fermentation broth of FIG. 1; FIG.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. However, the description of the present invention is merely an example for structural or functional explanation, and the scope of the present invention should not be construed as being limited by the embodiments described in the text. That is, the embodiments are to be construed as being variously embodied and having various forms, so that the scope of the present invention should be understood to include equivalents capable of realizing technical ideas. Also, the purpose or effect of the present invention should not be construed as limiting the scope of the present invention, since it does not mean that a specific embodiment should include all or only such effect.

The meaning of the terms described in the present invention should be understood as follows.

The terms "first "," second ", and the like are intended to distinguish one element from another, and the scope of the right should not be limited by these terms. For example, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component. It is to be understood that when an element is referred to as being "connected" to another element, it may be directly connected to the other element, but there may be other elements in between. On the other hand, when an element is referred to as being "directly connected" to another element, it should be understood that there are no other elements in between. On the other hand, other expressions that describe the relationship between components, such as "between" and "between" or "neighboring to" and "directly adjacent to" should be interpreted as well.

It should be understood that the singular " include "or" have "are to be construed as including a stated feature, number, step, operation, component, It is to be understood that the combination is intended to specify that it does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, unless otherwise defined. Commonly used predefined terms should be interpreted to be consistent with the meanings in the context of the related art and can not be interpreted as having ideal or overly formal meaning unless explicitly defined in the present invention.

Now, a cosmetic composition, a method of manufacturing a cosmetic composition and a method of manufacturing a cosmetic composition using the cosmetic composition according to an embodiment of the present invention will be described in detail with reference to the drawings.

FIG. 1 is a flow chart for explaining a method of manufacturing a cosmetic composition according to an embodiment of the present invention, FIG. 2 is a flowchart for explaining a process for producing a crude extract shown in FIG. 1, .

Referring to FIGS. 1 to 3, first, a crude extract is prepared by adding a solvent set to be gouge (S100).

In one embodiment, it is a perennial vine plant belonging to the genus Bacillus. It is grown in the Southeast Asian region (mainly Vietnam), is native to southern China and the northeastern part of Australia, and grows about 50 cm tall in a month after germination , White flowers, then become dark orange when the fruit is ripe. The time from seeding to harvest is about 9 months, and it is harvested mainly in December and January. This is a round or oval fruit of the size of a melon. It contains 30 to 40 red pulp containing seeds in a thick, hard shell with spiny protrusions. The hard shell prevents the fruit from decaying from mild weather. And also serves to protect the nutrients inside.

In one embodiment, Kaeuk's efficacy is enriched with antioxidants known to increase immunity in the body and effectively prevent aging of human cells and organs. Lycopene is 70 times more potent than tomatoes, (beta-carotine) was found to contain more than 10 times the sweet potato or carrots. In addition, it has been known that Kaeuk has high bioavailability and is excellent in the ability to absorb cells in the body.

In step S100, the crude extract is firstly washed with purified water, dried and pulverized (S110). (See Fig. 2)

In the above-described step S110, the grinding step (S110) may be performed by, for example, putting the gel into a container such as a beaker, a bowl, or the like, which is then filled with purified water, and washing the gel.

In the above-described step S110, the crushing step (S110) may be performed by using a crusher or a sieve (for example, a sieve of 30 to 100 mesh) (preferably a sieve of 50 mesh) have.

After the above-described step S110, ethanol is added to the crushed gecko and reflux-extracted (S120). (See Fig. 2)

In the above-described step S120, in the reflux extraction step (S120), 60% ethanol may be added so that the crushed gecko (that is, the crushed pulverized product) is 100 g / L before reflux extraction.

In the above-described step S120, the reflux extraction step (S120) may be performed by using a reflux extractor so that the pulverized product to which ethanol has been added is heated for a predetermined time (for example, 2 hours to 6 hours) ) (Preferably, twice) by a predetermined number of times (for example, 1 to 5 times).

In step S120, the ghiqui extracted by refluxing is filtered and dried to produce a ganoderma extract (S130). (See Fig. 2)

In step S130 described above, the geranium extract-producing step (S130) can be performed by transferring the reflux-extracted geranium to a filtration apparatus (preferably, whatman filter paper -No.2) after warming.

In step S130 described above, the crude extract may be prepared by freeze-drying the filtered gauze in a freeze-dryer, transferring the filtered gauze to a spray dryer, and spray-drying the sprayed gauze extract.

After the above-described process S100, the crude extract is fermented to produce a fermented liquid (S200).

In step S200, the fermentation broth (S200) is prepared by first adding glucose to the crude extract, inoculating the fermented mung bean culture, and fermenting the fermented fermentation broth to produce a first lean culture (S210) (see FIG. 3).

In step S210 described above, the first lean culture solution producing step (S210) comprises adding 3% glucose to 7% glucose (preferably 4% glucose) as a carbon source to 5 g to 20 g (preferably 10 g) Can be added.

In the above-described step S210, the first gecko broth culture producing step (S210) is performed by adding 5 L to 15 L (preferably 10 L) of the cultivation medium of Ganoderma lucidum strain to the extract of Ganoderma lucidum to which glucose is added, L. &Lt; / RTI >

In one embodiment, Ganoderma lucidum is used to stabilize the mind and body, to supplement the blood of the body, to calm the cough, and is used as a medicine for nervous breakdown, insomnia, dizziness, old cough, There is an efficacy that can prevent.

In step S210 described above, the first lean culture medium producing step (S210) is a step of producing the first lean media by adding the glucose-added Leucocephalum extract to the culture medium under aerobic conditions for a predetermined period (for example, 5 to 15 days (For example, pH 6 to pH 7) (preferably, pH 6.5) at a predetermined temperature (for example, 20 to 30 degrees Celsius (preferably 25 degrees Celsius) It can be fermented.

In one embodiment, the Geruk extract to which glucose is added is introduced into a fermenter (for example, a fermenter, a fermenter, etc.) (preferably a 10 L fermenter) ). &Lt; / RTI >

In the above-described step S210, the first step (step S210) is a step of putting the fermented goat's extract into a centrifuge, centrifuging it to remove the fermented microorganism, and then adding the fermented goat's extract to the sterilizer It can be sterilized under the set conditions so as not to be further cultured. Here, the predetermined condition is 1 to 2 atmospheres (preferably, 1.3 atmospheres (preferably atmospheric pressure) for a time of 5 minutes to 25 minutes (preferably 15 minutes) at a temperature of 120 to 140 degrees ). &Lt; / RTI >

After the above-described step S210, glucose is added to the first lean culture medium produced in step S210, and then the culture liquid of the mushroom strain is inoculated and fermented to produce a second lean culture (S220) (see FIG. 3).

In step S220, the second lean media preparation step (S220) is performed by adding 3% glucose to 7% glucose (preferably 4% glucose) as a carbon source to the first lean media, The culture medium may be added to a volume of 5 L to 15 L (preferably, 10 L) so that the first lean medium supplemented with glucose is 30 g / L.

In the above-described step S220, the second lean media preparation step (S220) is a step in which the first lean media to which glucose is added is cultured under aerobic conditions for a predetermined period (for example, 5 to 15 days) (Preferably pH 6 to pH 7) (preferably pH 6.5) at a predetermined temperature (for example, 20 to 30 ° C) (preferably, 27 ° C) And can be fermented.

In one embodiment, the first lean culture medium to which glucose is added is introduced into a fermenter (for example, a fermenter, a fermenter, a fermenter) (preferably a 10 L fermenter) A sufficient supply condition).

After the above-described step S220, ethanol is added to the second lean culture medium produced in the above-described step S220, filtered and dried to produce a lean fermented broth (S230) (see FIG. 3).

In the above-described step S230, the step of fermenting fermentation broth (S230) Ethanol was added to the second lean culture and then The ethanol is added to the second suckling culture solution to which ethanol has been added for a predetermined number of times (for example, 2 to 7 hours) (preferably 5 hours) (Preferably, three times).

In step S230, the second fermentation broth obtained by refluxing is cooled and then transferred to a filtration apparatus (preferably, whatman filter paper-No.3) to be filtrated have.

In the above-described step S230, the step (S230) of producing the fermentation broth is carried out by transferring the filtered second coquetrification broth to a concentration apparatus (for example, a concentration tank) at a predetermined temperature (for example, And then concentrated under reduced pressure.

In the above-described step S230, in the step (S230) of producing the fermentation broth, the second confluence broth concentrated under reduced pressure is transferred to a freeze-dryer, and then freeze-dried to produce a fermented broth.

The cosmetic composition according to an embodiment of the present invention can be used as a cosmetic composition for a cosmetic composition, such as a gecko fermentation broth, a platinum colloid aqueous solution, purified water, glycerin, a phygid-10 dimethicone / vinyl dimethicone crosspolymer, cyclopentasiloxane, butyleneglycol, Phenoxyethanol, Piggy-10 Dimethicone, Propolis Extract Flavor, Phellinus linteus Extract, Allantoin, Prickly Pear Fruit Extract, Disodium Iodide, Benzophenone-5, Water Flower Extract, Papaya Fruit Extract, Bamboo leaf extract, saponin, and green tea extract may be mixed with each other.

In one embodiment, the cosmetic composition comprises 0.02-0.16% by weight of a Gecko fermentation broth, 50% by weight of a platinum colloid aqueous solution, 48.01-49.76% by weight of purified water and glycerin, 0.01-0.30% by weight of a phage-10 dimethicone / vinyl dimethicone crosspolymer 0.01 to 0.08% by weight of cyclopentasiloxane and butylene glycol, 0.01 to 0.08% by weight of gum extract and sodium chloride, 0.01 to 0.08% by weight of phenoxyethanol, 0.01 to 0.08% by weight of phage-10 dimethicone, 0.01 to 0.08% by weight of a propolis extract, 0.01 to 0.08% by weight of a propolis extract, 0.01 to 0.08% by weight of an aspartic acid extract, 0.01 to 0.08% by weight of an allantoin, 0.01 to 0.08% 0.01 to 0.08% by weight, 0.01 to 0.08% by weight of benzophenone-5, 0.01 to 0.08% by weight of a water lily flower extract, 0.01 to 0.08% by weight of papaya fruit extract, 0.01 to 0.08% by weight of dipropylene glycol, 0.01 to 0.08% by weight of squalane, 0.01 to 0.09% by weight of cynthia liquorice extract, 0.01 to 0.0 The bamboo leaf extract is 0.01 to 0.09% by weight, the saponin is 0.01 to 0.09% by weight and the green tea extract is 0.01 to 0.09% by weight. Lt; / RTI >

In one embodiment, the cosmetic composition is prepared by dissolving 0.02 to 0.16 (mg) of a crude fermentation broth, 50 (mg) of a platinum colloid aqueous solution, 48.01 to 49.76 (mg) of purified water and glycerin, (Mg) of cyclopentasiloxane and butylene glycol and 0.01 to 0.08 (mg) of sodium chloride and 0.01 to 0.08 (mg) of phenoxyethanol, 0.01 to 0.08 mg of dimethicone crosspolymer, 0.01 to 0.08 And the allantoin is 0.01 to 0.08 (mg), the fat-10 dimethicone is 0.01 to 0.08 (mg), the propolis extract flavor is 0.01 to 0.08 (mg), the march extract is 0.01 to 0.08 (mg) (mg), extracts of Pricklyearia fruit were 0.01 to 0.08 (mg), disodium ditallow was 0.01 to 0.08 (mg) and benzophenone-5 was 0.01 to 0.08 (mg) 0.01 to 0.08 (mg), papaya fruit extract to 0.01 to 0.08 (mg), dipropylene glycol to 0.01 to 0.08 (mg), squalane to 0.01 to 0.08 (mg) (0.01 to 0.09 (mg)), the extract of licorice (0.01 to 0.09 mg), the extract of Angelica koreana (0.01 to 0.09 mg), the extract of broiler chickens (0.01 to 0.09 mg) and the extract of bamboo leaf mg), saponin in an amount of 0.01 to 0.09 (mg), and green tea extract in an amount of 0.01 to 0.09 (mg).

In one embodiment, the cosmetic composition may function to prevent skin aging, such as cucurbit extract, licorice extract, hwanggi extract, broiler extract, bamboo leaf extract, saponin, and green tea extract.

In one embodiment, the cosmetic composition may contain 0.0001 to 100.0% by weight (preferably 0.1 to 50% by weight) of the fermentation broth according to the total weight.

In one embodiment, the antioxidant effect of the cosmetic composition may be insufficient when the content of the fermentation broth is less than 0.0001% by weight based on the total weight.

The method of manufacturing a cosmetic product using the cosmetic composition according to an embodiment of the present invention may be applied to a cosmetic composition containing a softening substance, a nutrition product, an essence, a nutritional cream, a pack, a lotion, a gel, a water-soluble liquid, a cream, an emulsion, , Lipstick, and face powder, and one selected from the group consisting of a cosmetic composition is mixed with the selected one, and one cosmetic composition is formulated into a cosmetic product.

In a cosmetic composition manufacturing method using a cosmetic composition, a classification selector selects one from the group consisting of a softening article, a nutrition toilet, an essence, a nutrition cream, and a pack, and delivers the selected one to the mixer. The mixer then mixes the selected one with the cosmetic composition and delivers it to the formulator. The formulator can then be formulated into a cosmetic with one containing the cosmetic composition.

In one embodiment, a comparison table according to the mixing components of the Gecko fermentation broth is shown in Table 1.

In one embodiment, the free radical scavenging activity was measured using the DPPH method to confirm the antioxidative effect of the second goethite culture solution.

In one embodiment, the DPPH method is a method of measuring the absorbance at 517 nm to the extent that the inhibitor decolorizes the stable radical DPPH (2,2-Diphenyl1-1-picry1 hydrazyl1 radical).

In one embodiment, the equation for calculating the DPPH method is shown in Equation (1). (Fermentation broth), Comparative Example 1 (fermentation broth), Comparative Example 2 (fermentation broth), Comparative Example 3 (fermentation broth) and Comparative Example 4 (fermentation broth) B is the reaction absorbance of the blank sample solution.

In one embodiment, the free radical is a causative agent of aging, particularly skin aging. The free radical scavenging activity of the second leukocyte is measured by the DPPH method using 1 ml of 0.2 mM DPPH solution in methanol , 1 ml of ethanol was added, 1 ml of various concentrations of extract was added, and the mixture was allowed to stand at room temperature for 10 minutes. Then, the absorbance was measured at 517 nm by a spectrometer. The activity of the DPPH was determined by the following equation (2). The activity of DPPH was determined by the experimental group (control) and the sample without the sample. Where A is the absorbance of the negative control without treatment of the second leukocyte of the invention and B is the absorbance of the negative control treated with the second leukocyte.

In one embodiment, the percent free radical scavenging effect according to the example of the second leukocyte solution and the comparative example is shown in Table 2.

In Table 2, it can be seen that the free radical scavenging effect of the second goethite culture broth prepared in the Examples is superior to the free radical scavenging effect of the Gecko fermentation broth and goatee extract prepared in Comparative Examples 1 to 4. In other words, it can be confirmed that the primary fermentation with Ganoderma lucidum and the secondary fermentation with the secondary mushroom showed the highest free radical scavenging activity.

The method (S10) for producing a cosmetic composition having the above-described constitution is a method for producing a cosmetic composition by fermenting Gewooke so as to produce a fermentation broth and adding cosmetic fermented with a fermented tea fermentation solution to produce a cosmetic composition containing a large amount of lycopene, Contains antioxidants to effectively prevent aging of human cells and organs. In other words, it is possible to extract lycopene and beta-carotene from Kaeuk by mixing cosmetic composition by mixing Kaeuk fermentation broth with platinum colloid, and the extracted lycopene and beta carotene exert a greater synergy effect on the removal of active oxygen of platinum, The root cause can be prevented.

As described above, the embodiment of the present invention is not limited to the above-described apparatus and / or method, but may be implemented by a program for realizing a function corresponding to the configuration of the embodiment of the present invention and a recording medium on which the program is recorded And the present invention can be easily implemented by those skilled in the art from the description of the embodiments described above. While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, It belongs to the scope of right.

Claims

A process for producing a crude extract by adding a predetermined solvent to a crude extract; And fermenting the Geruk extract to produce a fermentation broth.
The process for producing the above-
Producing a first crude culture medium by adding glucose to the crude extract and then inoculating and fermenting the culture medium of the ginger mushroom to produce a first lean culture;
Producing a second lean culture medium by adding glucose to the first lean culture medium and then inoculating and culturing the culture medium of the mushroom strain to produce a second lean culture; And
And adding ethanol to the second growth medium to be filtered, followed by drying to produce a fermentation broth.

The method according to claim 1,
A crushing step of washing and drying crushed kaolin with purified water;
Reflux extraction step in which ethanol is added to the crushed gecko and reflux extraction is carried out; And
And a step of producing a goethite extract by filtration after the reflux-extraction step and then drying the resultant to produce a goethite extract.

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2. The method according to claim 1,
Wherein the crude extract is fermented at a predetermined pH and maintained at a predetermined temperature for a predetermined period of time.

2. The method according to claim 1,
Wherein the fermented goethite extract is centrifuged and sterilized to produce a first crude culture liquid.

2. The method according to claim 1,
Wherein the first lean culture is fermented at a predetermined pH and maintained at a predetermined temperature for a predetermined period of time.

2. The method of claim 1,
Ethanol is added to the second leukocyte culture, refluxing is carried out for a predetermined number of times, followed by cooling and filtration.

2. The method of claim 1,
A method for producing a cosmetic composition, which comprises concentrating a crude fermentation broth under reduced pressure and freeze-drying it.

10 dimethicone / vinyl dimethicone crosspolymer, cyclopentasiloxane, butyleneglycol, succinic acid extract, sodium chloride, phenoxyethanol, phage-10 dimethicone, Propolis extract, fragrance, extract of michi, allantoin, prickly pear fruit extract, disodium edithia, benzophenone-5, water lily flower extract, papaya fruit extract, dipropylene glycol, squalane, licorice extract, licorice extract, A green tea extract, a green tea extract, a bamboo leaf extract, a saponin, and a green tea extract.

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