KR101821502B1 - mushroom liquid spawn inoculation method - Google Patents

Abstract

The present invention relates to a mushroom liquid spawn inoculation method, and specifically, the present invention relates to a method of inoculating a mushroom liquid spawn, which can improve workability and greatly improve air permeability so as to easily carry out bag insertion and spawn injection processes in the cultivation of mushrooms in bags, thereby greatly improving mycelial cultivation and rooting efficiency. The mushroom liquid spawn inoculation method comprises a step of forming a through-hole penetrating a cylindrical medium, a step of inserting the medium into a plastic bag, a step of inoculating a mushroom liquid spawn, a step of preparing a plurality of ventilation pipes, a step of connecting the ventilation pipes to the through-hole, and a step of blocking the upper opening of the ventilation pipes using air-permeable members.

Description

{Mushroom liquid spawn inoculation method}

The present invention relates to a method of inoculating a liquid mushroom, and more particularly, it relates to a method for inoculating a mushroom liquid, The present invention relates to a method of inoculating a liquid mushroom.

Generally, the cultivation methods of mushrooms are somewhat different depending on their kinds, but they are mixed with various media such as woody, cultivated using cotton seeds and rice straw, sawdust, cottonseed, rice bran, etc. and filled in containers or bags Container cultivation and encapsulation cultivation methods.

In the case of bag cultivation, the mushroom seedlings are inoculated into the medium, and the mushroom is cultured through the incubation step. At this time, a lid for cultivation of mushroom seed culture is installed in the bag for entering the culture medium, in order to introduce outside air required for mycelial breathing and to discharge carbon dioxide generated from the culture medium to the outside.

More specifically, in the cancer incubation step, the culture medium is periodically ventilated to control the temperature of the culture medium so as not to exceed 25 ° C in order to prevent damage by mycelial respiration heat and to release carbon dioxide generated during mycelial respiration. When the cancer culture step is completed, mushroom mycelium is activated and the surface of the culture medium is whitened. Next, when the dark culture is completed, the culture is switched to light culture to induce browning.

For example, as shown in FIG. 13, a registration hole 20-0477853 has a through hole 14 formed at the center of the upper surface of a lid body 10 having an inward screw 11 formed therein, and an inner space The receiving wall 16 is formed so as to extend upward to allow the filter 20 to be inserted into the receiving space 17. The receiving wall 16 is formed along the upper surface of the receiving wall 16 so that the diameter of the receiving space 17 And a plurality of air circulation portions 18 are formed along the perimeter of the receiving wall 16 so that the inside and the outside air of the culture container can be flowed through the filter 20 The air circulating part 18 is formed by removing a part of the air circulating part 18 from the outer wall and the upper part of the receiving wall 16 so as to form a space. On the upper part of the lid body 10, 32) in a radial direction, and a top cover (30) The outer peripheral lower end face 36 of the upper cover 30 is connected to the upper end face 18a of the air circulation portion 18 of the lid body 10 when the upper end lid 30 is coupled to the upper portion of the lid body 10 And the lower part of the lid body 10 is screwed to an inward screw threaded with a lower coupling body 40 to be used as a substitute for a culture container. And is configured to be applicable to various large-capacity culture pouches (50).

Such a lid for cultivating a mushroom seed culture has a disadvantage in that it is complicated in structure and expensive in manufacturing cost.

Further, as shown in FIG. 13, the loaded medium is cultivated to induce the mushroom to grow only at the upper part of the medium, and thus the yield of the medium is low. In the case of cultivating the medium in the form of a rectangular cylinder, it was difficult to install a lid for ventilation, so a hole was made at the top of the bag and a paper tape for ventilation was attached. However, in this case, there has been a problem that the air permeability is lowered and the period of time of mycelial growth is greatly increased.

Korean Utility Model Registration No. 20-0477853 Korean Patent No. 10-1174245 Korean Patent No. 10-1640472

It is an object of the present invention to overcome the above-mentioned problems, and it is an object of the present invention to provide a method for cultivating mushroom bags, And to provide a method of inoculating liquid mushroom which can greatly improve the efficiency.

The problems to be solved by the present invention are not limited to those mentioned above, and other solutions not mentioned can be clearly understood by those skilled in the art from the following description.

As a means for achieving the object of the present invention, the method for inoculating a mushroom liquid koji according to the present invention comprises the steps of: providing a cylindrical culture medium and forming a through hole penetrating the bottom surface and the upper surface of the culture medium; Sealing the medium in a bag; Injecting a liquid seedling while inserting a liquid seedling inoculation unit at a predetermined interval on an outer circumferential surface of the loaded seedlings while lying down; Providing a plurality of vent pipes that are completely opened up and down; Inserting the vent tube at each point where the liquid seed is injected so that the lower opening of the vent tube is in communication with the through hole of the medium; And closing the upper opening of the vent pipe with an air permeable member.

In the method for inoculating a mushroom liquid koji according to the present invention, the vent pipe comprises an insertion tube portion having a tapered cross section that becomes narrower from the upper opening portion toward the lower opening portion, and a stationary ring portion extending in the lateral direction from the rim of the upper opening portion .

In addition, the method for inoculating a mushroom liquid koji according to the present invention comprises the steps of: providing a cylindrical-shaped culture medium and forming a through-hole penetrating through a bottom surface and an upper surface of the culture medium; Sealing the medium in a bag; Providing a plurality of vent pipes that are completely opened up and down; Inserting a plurality of vent tubes at predetermined intervals on an outer circumferential surface of the loaded medium in a state of being laid down laterally; Injecting liquid seeds into each ventilator with a liquid seeding device; And closing the upper opening of the vent pipe with an air permeable member, wherein a plurality of holes are formed on an outer circumferential surface of the vent pipe.

Further, in the mushroom liquid seeding method according to the present invention, the vent tube is inserted such that the lower opening communicates with the through hole of the medium, a part of the injected liquid seed is passed through the hole part, And flows into the through hole.

In addition, in the method for inoculating a mushroom liquid koji according to the present invention, it is preferable that the vent tube has an insertion tube portion having a tapered cross-section that becomes narrower from the upper opening portion to the lower opening portion and the hole portion is formed on the outer circumferential surface, And a stationary ring portion extending in the direction of the arrow.

Further, in the method for inoculating a mushroom liquid koji according to the present invention, the through-hole is formed so as to penetrate the center of the bottom surface and the top surface of the medium.

In the method for inoculating a mushroom liquid koji according to the present invention, a plurality of through holes are formed, and the above-mentioned vent pipe is divided into a plurality of through holes.

According to the method of inoculating the mushroom liquid koji according to the present invention having the above-described structure, it is possible to improve the workability and to greatly improve the air permeability so that the mushroom bag cultivation can easily carry out the pouring and seed infusion process, There is an effect that can be made.

The effects of the present invention are not limited to those mentioned above, and other solutions not mentioned may be clearly understood by those skilled in the art from the following description.

1 is a block diagram showing a first embodiment of a method of inoculating a liquid mushroom inoculation according to the present invention.
2A and 2B are a perspective view and a photograph showing a vent pipe of the present invention.
3 is a perspective view showing a state in which a vent pipe is inserted into the medium of the present invention.
4 is a cross-sectional view of Fig.
5 is a block diagram showing a second embodiment of a method of inoculating a mushroom liquid koji according to the present invention.
6 is a perspective view showing a vent pipe having a structure different from that of FIG.
7 is a perspective view showing a state in which a vent pipe is inserted into the medium of the present invention.
Fig. 8 is a sectional view of Fig. 7. Fig.
9 is a perspective view showing a state in which a plurality of through holes are formed in the medium of the present invention and a vent pipe is inserted into each through hole.
Figs. 10 to 12 are photographs showing the cultivation in the medium inoculated with the mushroom liquid seedlings in Examples 1 and 2 and Comparative Example 1. Fig.
Fig. 13 is a view showing a lid for culturing mushroom seeds used in conventional mushroom bag cultivation.

Hereinafter, embodiments of the present invention will be described in more detail with reference to the accompanying drawings.

In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. In addition, the terms described below are defined in consideration of the functions of the present invention, and these may vary depending on the intention of the user, the operator, or the precedent. Therefore, the definition should be based on the contents throughout this specification. In the description of the present invention, the same or similar reference numerals are given to the same or similar components, and a detailed description thereof will be omitted.

Fig. 1 is a block diagram showing a first embodiment of a method of inoculating a mushroom liquid koji according to the present invention, Figs. 2a and 2b are a perspective view and a photograph showing a vent pipe of the present invention, FIG. 4 is a cross-sectional view of FIG. 3. FIG.

1 to 4, the method of inoculating a mushroom liquid koji according to the present invention is configured to greatly improve air permeability in the case of mushroom bag cultivation, and comprises largely steps S1 to S6, There are no special restrictions on mushrooms.

1, the method for inoculating mushroom liquid koji according to the present invention is characterized in that a cylinder-shaped culture medium 10 is provided and S1 (S1) forming a through-hole 11 penetrating the bottom surface and the upper surface of the culture medium 10 A step S2 of inserting the medium 10 into the bag 13, a step S3 of injecting liquid seeds into the medium 10, a step S4 of providing a plurality of ventilation pipes 100 which are completely opened up and down, (S5) of inserting the vent pipe (100) into the medium (10), and closing the upper opening (103) of the vent pipe (100) with the air permeable member (15).

The reason why the through hole 11 is formed in the medium 10 in the step S1 is that the vent pipe 100 is inserted and communicated so that the carbon dioxide generated during the mushroom cultivation process can be discharged more efficiently .

That is, the through-hole 11 is a space completely passing through the mushroom cultivation medium 10 and communicates with the lower end of the vent pipe 100. The carbon dioxide generated in the medium 10 is temporarily stored in the through hole 11 and then discharged to the outside through the vent pipe 100.

When the diameter of the culture medium is 12 cm, the diameter of the through hole is 0.5 to 1.5 cm.

After the through hole 11 is formed in the medium 10 in the step S1, the step S2 of sealing the bag is performed. Here, the bag 13 may be made of a vinyl material.

In step S3, the liquid seedling is injected while inserting the liquid seedling inoculation device at a predetermined interval on the outer circumferential surface in a state in which the loaded medium 10 is laid down laterally.

Referring to FIG. 2, the vent pipe 100 of the present invention has a completely opened upper and lower openings. The lower opening 105 is disposed in the through hole 11 to allow the carbon dioxide contained in the through hole 11 to flow to the outside And serves to regulate the humidity and the discharge, and can be largely composed of the insertion tube portion 101 and the stationary ring portion 109.

The insertion tube portion 101 may have a height corresponding to the radius of the culture medium 10. For example, when the culture medium has a diameter of 12 cm, the insertion tube portion may have a height of 6 ± 0.5 cm.

It is preferable that the insertion tube portion 101 has a tapered cross section that becomes narrower from the upper opening portion 103 to the lower opening portion 105 so that the insertion tube portion 101 can be easily inserted into the medium 10. When the upper opening portion is 15 mm, Can be 10 < RTI ID = 0.0 > 3mm. ≪ / RTI >

The fixed ring portion 109 extends transversely at the rim of the upper opening portion 103 and acts as a step for guiding the depth of the vent pipe 100. The width of the fixed ring portion 109 is 5 to 10 mm have.

In the step S5, the vent pipe 100 is inserted into the adhered medium 10 to allow the lower opening 105 of the vent pipe 100 to communicate with the through hole 11 of the medium 10. Here, the vent pipe 100 may be inserted at each point where the liquid seed is injected, but not always limited thereto, and may be inserted at a point where no liquid seed is injected.

Finally, in step S5, when the upper opening part 103 of the vent pipe 100 is closed with a breathable member 15 such as cotton, the liquid seeding is completed.

Fig. 5 is a block diagram showing a second embodiment of a method of inoculating a mushroom liquid culture according to the present invention, Fig. 6 is a perspective view showing a vent pipe having a structure different from that of Fig. 2, FIG. 8 is a cross-sectional view of FIG. 7. FIG.

5 to 8, the method for inoculating a mushroom liquid koji according to the present embodiment includes providing a cylindrical culture medium 10, forming a through-hole 11 penetrating the bottom surface and the upper surface of the culture medium 10 (S12) of inserting the medium (10) into a bag, S13 (S13) of providing a plurality of ventilation pipes (100) completely opened up and down, A step S14 of inserting a plurality of vent tubes 100 at an outer peripheral surface at predetermined intervals, a step S15 of injecting liquid seeds into the vent tubes 100 with a liquid seeding machine, Blocking with the member 15 may include step S16.

The vent pipe 100 has an insertion tube portion 101 having a tapered cross section tapered from the upper opening portion 103 to the lower opening portion 105 and having the hole portion 107 formed on the outer peripheral surface thereof, And a stationary ring portion 109 extending in the transverse direction at the rim thereof.

This embodiment differs from the above-described embodiment in that a plurality of holes 107 are formed on the outer circumferential surface of the vent pipe 100.

That is, in the present embodiment, the vent pipe 100 not only plays a role of securing air permeability at the time of mushroom bag cultivation but also serves as a flow path for injecting liquid seeds.

More specifically, the lower opening 105 of the vent pipe 100 is inserted so as to communicate with the through hole 11 of the medium 10, thereby not only discharging the carbon dioxide contained in the through hole 11, And also through a plurality of holes 107 communicating with the inner wall 101. [

A part of the liquid germs injected in the step S15 passes through the hole 107 and the remaining part of the liquid germs flows into the through hole 11 through the lower opening 105, So that it can be uniformly injected.

9 is a perspective view showing a state in which a plurality of through holes are formed in the medium of the present invention and a vent pipe is inserted into each through hole.

Although one through-hole 11 is formed in the center of the medium 10 in the above-described embodiment, the present invention is not limited thereto. As shown in FIG. 9, a plurality of through-holes 11 may be formed And the vent tube 100 may be configured to be inserted into the plurality of through holes 11 in a divided manner.

As described above, when there are a plurality of through holes, the discharge of carbon dioxide, humidity control, and air permeability generated in the medium significantly increase.

Hereinafter, a more preferred embodiment and a comparative example of the mushroom liquid seeding method according to the present invention will be described in more detail.

First, a sawdust medium having a diameter of 12 cm and a height of 50 cm is prepared, and a through hole having a diameter of 1 cm is formed at the center.

Next, the medium is sealed in a bag, and the liquid mushroom liquid seed is injected into four points on the top of the medium using a liquid seeding machine.

Then, a vent tube having a diameter of the upper opening of 15 mm, a diameter of the lower opening of 10 mm, and a height of 6 cm was provided, inserted into the four points inoculated with the liquid seeds, and the seed opening was closed at the upper opening to complete the seedling inoculation.

The seedling was inoculated in the same manner as in Example 1, except that a plurality of holes were formed on the outer circumferential surface of the vent tube, then inserted into the medium, and injected with the mushroom liquid seeds through the upper opening of the vent tube.

[Comparative Example 1]

The seedling was inoculated in the same manner as in Example 1 except that four holes were made in the bag and a paper tape was attached to the hole instead of inserting the through-hole into the medium without forming the through hole in the medium.

[Experimental Example 1]

Table 1 below shows the results of measuring the time of completion of the whitening in which the surface of the culture medium is completely whitened by the mycelial growth in the medium inoculated with the mushroom liquid seedlings in Examples 1 and 2 to Comparative Example 1. [

Here, the media used in Examples 1 and 2 to Comparative Example 1 were the same size, and the amounts of liquid seeds to be injected were the same, and cultured in the same incubation room at the same temperature.

10 to 12 are photographs showing the cultivation in the medium inoculated with the mushroom liquid seeds in Examples 1 and 2 and Comparative Example 1. Fig.

Comparative Example 1 Example 1 Example 2 Backslip completion period 51 days 46 days 42 days

As can be seen from the above Table 1, it was confirmed that the rate of mycelial growth was significantly increased in Examples 1 and 2 in which the vent tube of the present invention was inserted. Particularly, in Example 2, it was confirmed that a plurality of hole portions were formed on the outer circumferential surface of the vent tube, and that when the liquid seed was injected into the hole portion, the perfusion complete period was the shortest, It was judged that the rate of transition of the mycelial growth was higher than that of Example 1 and the breathability was improved.

It is to be understood that the invention is not limited to the form set forth in the foregoing description. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims. It is also to be understood that the invention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.

10: Medium 11: Through hole
13: bag 15: breathable member
100: vent pipe 101:
103: upper opening 105: lower opening
107: hole portion 109: fixed ring portion

Claims (7)

In a mushroom liquid seeding method,
Providing a cylindrical-shaped culture medium and forming a through-hole penetrating the bottom surface and the upper surface of the culture medium;
Sealing the medium in a bag;
Injecting a liquid seedling while inserting a liquid seedling inoculation unit at a predetermined interval on an outer circumferential surface of the loaded seedlings while lying down;
Providing a plurality of vent pipes that are completely opened up and down;
Inserting the vent tube at each point where the liquid seed is injected so that the lower opening of the vent tube is in communication with the through hole of the medium;
Closing the upper opening of the vent pipe with a breathable member;
≪ / RTI >
The method according to claim 1,
The air-
Wherein the inserting tube portion comprises an insertion tube portion having a tapered cross section that becomes narrower from an upper opening portion to a lower opening portion and a retaining ring portion extending in a lateral direction from a rim of the upper opening portion.
In a mushroom liquid seeding method,
Providing a cylindrical-shaped culture medium and forming a through-hole penetrating the bottom surface and the upper surface of the culture medium;
Sealing the medium in a bag;
Providing a plurality of vent pipes that are completely opened up and down;
Inserting a plurality of vent tubes at predetermined intervals on an outer circumferential surface of the loaded medium in a state of being laid down laterally;
Injecting liquid seeds into each ventilator with a liquid seeding device;
And closing an upper opening of the vent pipe with an air permeable member,
And a plurality of holes are formed on an outer circumferential surface of the vent pipe.
The method of claim 3,
Characterized in that the vent pipe is inserted such that a lower opening thereof communicates with a through hole of the culture medium, and a part of the injected liquid species passes through the hole and the remaining part flows into the through hole. Way.
The method of claim 3,
The air-
And a fixing ring portion extending in the transverse direction from the rim of the upper opening portion, wherein the insertion tube portion has a tapered cross section that becomes narrower from the upper opening portion to the lower opening portion, Inoculation method.
The method according to any one of claims 1 to 5,
Wherein the through hole is formed to penetrate the center of the bottom surface and the top surface of the culture medium.
The method according to any one of claims 1 to 5,
A plurality of through holes are formed,
Wherein the vent pipe is divided into a plurality of through holes.

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