KR101794026B1

KR101794026B1 - Manufacturing method of kudzu leaf soap

Info

Publication number: KR101794026B1
Application number: KR1020150156722A
Authority: KR
Inventors: 양수진; 양임회; 강미녀; 양순희; 강성희
Original assignee: 양수진
Priority date: 2015-11-09
Filing date: 2015-11-09
Publication date: 2017-11-06
Also published as: KR20170054040A

Abstract

The present invention relates to a process for producing natural soap using a sesame leaf. Specifically, the sesame leaf is fermented into Lactobacillus acidophilus, Bacillus subtilis, and Saccharomyces cerevisiae to obtain a perilla leaf fermented product, And a method for producing the same.
According to the present invention, it is possible to produce a soap which is very beneficial for the beauty of the skin, as it has excellent stability and feeling against skin irritation and exerts antioxidative effects.

Description

{Manufacturing method of kudzu leaf soap}

The present invention relates to a process for producing natural soap using a sesame leaf. Specifically, the sesame leaf is fermented into Lactobacillus acidophilus, Bacillus subtilis, and Saccharomyces cerevisiae to obtain a perilla leaf fermented product, And a method for producing the same.

Soap is a typical detergent used to remove stubborn skin, but it can be thought of as just cleaning, but given its direct contact with the skin, a very useful tool that can provide beneficial ingredients to the skin Or it may cause skin problems or skin diseases. Accordingly, many people think that soap is not simply a cleaning agent but a beauty product such as cosmetics or a health product that protects against skin diseases.

Soaps are being developed that incorporate a variety of functional materials to provide beneficial effects to the skin as they become recognized as important to the health of the skin. For example, Korean Patent No. 10-0996882 discloses a soap having improved moisturizing power and cleansing power by incorporating brown algae powder into soap. In Korean Patent No. 10-0851954, bamboo is heated at a high temperature, A soap excellent in cosmetic effect, moisturizing effect, exfoliation effect and the like is disclosed.

On the other hand, Pueraria lobata (Willd.) Ohwi is a perennial soybean and vine plant commonly found throughout Korea. It does not die well even in the cold of winter and it grows thick every year. Its length is usually 10 ~ 20m. It grows by stretching its stem. In the course of its stretching, it grows around a tree-like object with a stem.

There are many catechins, polyphenolics, and they are known to contain antioxidants and sedatives, and phytoestrogens that increase the activity of estrogen. The isoflavone derivatives puerarin, daidzein and genistein are abundant in root, stem, leaf, flower and seed, among which the highest activity in the root . Antioxidant, antioxidant, antihypertensive, anti-ulcer, and liver protective actions are known, and many studies have been conducted on the root of the root of the plant with the highest estrogenic activity to date.

In general, it is divided into roots (purple roots), flowers (gallbladder) and leaves (galloping). According to recent research results, it has been reported that antioxidant, anti-inflammation and whitening efficacy are superior in the above ground parts except roots.

The present inventor has developed a novel functional soap which is effective for skin care and can be safely used without side effects, by applying the ointment to the soap so that the useful effects of the ointment can be exerted and the skin irritation can be minimized.

Korean Patent No. 10-0996882 Korean Patent No. 10-0851954

Accordingly, a main object of the present invention is to provide a soap which is effective for skin care and can be safely used without side effects and a method for producing the same.

In accordance with one aspect of the present invention, the present invention provides a method of producing a fermentation product comprising: a) fermenting a sesame leaf with Lactobacillus acidophilus , Bacillus subtilis , and Saccharomyces cerevisiae , Obtaining a fermented product; And b) preparing a soap by adding the perilla leaf fermented product.

According to the present invention, when the sesame leaf is fermented with the above three microorganisms, it is possible to exhibit beneficial effects on the skin such as antioxidative effect when applied to soap, reduce skin irritation and feel a good feeling of use.

For effective perilla leaf fermentation, the perilla leaves are preferably pulverized and mixed with water to ferment, and it is preferable to further mix the carbon sources so that the fermenting microorganisms can grow to an appropriate level and survive stably. As the carbon source, a carbohydrate that can be used by all three microorganisms can be used, and a monosaccharide or a disaccharide such as glucose or sugar is preferably used. The mixture of perilla leaf powder, carbon source and water is preferably 200 to 600 parts by weight of carbon source and 10,000 to 20,000 parts by weight of water based on 100 parts by weight of perilla leaf powder for effective fermentation of perilla leaf powder. If the concentration is out of the above range, the fermentation efficiency is significantly lowered due to the inhibition of the growth of the fermenting microorganism or the excessive growth thereof, or the concentration of the component derived from the sesame leaf is not sufficient, so that the effect of the finally prepared soap may be lowered. More preferably 300 to 500 parts by weight of carbon source and 14,000 to 16,000 parts by weight of water based on 100 parts by weight of the sesame leaf powder.

As described above, the three microorganisms can be inoculated into the fermentation medium containing the perilla leaf powder, carbon source, and water, and the fermentation can be performed by adding the culture medium of each microorganism to the fermentation medium . Three microorganisms may be preincubated in one culture medium and inoculated with the culture medium. However, since the specific microorganism may have a dominant phenomenon during the pre-culture, it is preferable to pre-cultivate each microorganism separately. As the pre-culture medium, a medium conventionally used for culturing each microorganism can be used.

The temperature at the time of fermentation after inoculation is preferably 30 to 40 캜, more preferably 33 to 37 캜, for stable survival and fermentation of all three microorganisms. The fermentation time is preferably from 1 to 10 days for sufficient production of components exhibiting the effect of the present invention, more preferably from 4 to 6 days.

When the sesame leaf is fermented in the same manner as described above, a perilla leaf fermented product containing a large amount of components beneficial for skin can be obtained. It is preferable to use the sesame leaf fermented product by removing the solid content by centrifugation or filtration.

In the method of manufacturing a soap of the present invention, the step a) comprises: a-1) pulverizing a sesame leaf to produce a sesame leaf powder; a-2) 200 to 600 parts by weight of sugar and 10000 to 20000 parts by weight of water are mixed with 100 parts by weight of the perilla leaf powder to prepare a medium for fermentation of a leafy leaf; And a-3) Lactobacillus acidophilus , Bacillus subtilis , and Saccharomyces cerevisiae are inoculated into the medium for fermentation of leafy leaves and cultured at 30 to 40 DEG C And fermenting the fermented product for 1 to 10 days to produce a fermented product.

In the method for manufacturing a soap of the present invention, it is preferable that the soap is produced by adding the perilla leaf fermentation product to 1 to 12 wt% of the total weight of the soap composition in the step b). If the added amount of the perilla leaf fermented product is more than the added amount, the cleaning effect or physical properties inherent to the soap may be greatly lowered, and if the added amount is too small, the effect of the present invention may be significantly lowered. Also, if too much sesame leaf fermentation product is used, traces may be generated too quickly during the soap production process, which may make it difficult to produce soap.

In the method for manufacturing a soap according to the present invention, it is preferable to add a sesame leaf powder or a sesame leaf extract to the soap in the step b). If you make soap by adding sesame leaf powder or sesame leaf extract, you can appeal to people who use sesame leaves to include the sesame leaf in the soap.

It is preferable to add 0.1 to 1% by weight of the total weight of the soap composition in the case of adding the sepiolite powder so as to exhibit the effect of the sepiolite powder as described above without greatly affecting the washing effect and physical properties inherent in the soap.

In addition, when the sesame leaf extract is added, it is preferable to use an extract of sesame leaf extracted with 50 to 90% (v / v) ethanol as the sesame leaf extract and 0.01 to 0.1% by weight of the total weight of the soap composition based on the dry weight of the extract, So that the effect of the sesame leaf powder can be exhibited without significantly affecting the cleaning effect and physical properties inherent to the soap.

In the method for producing soap of the present invention, the step (b) is a step of preparing a soap by adding a sesame leaf fermentation product to the soap raw material. In this case, a soap raw material commonly used as a soap raw material may be used. For example, oil and sodium hydroxide can be used as raw materials for soap, and examples of the oil include ordinary oils that can be used as a raw material for soap such as olive oil, palm oil, palm kernel oil, coconut oil, macadamia nut oil, , Sunflower seed oil, camellia oil, avocado oil, soybean oil, grape seed oil, sweet orange oil, petit train oil, rosemary oil and cottonseed oil may be used alone or in combination. It may contain other excipients which can be added normally to the soap, for example, salt, anhydrous anhydrous and talc, to the extent that the effects of the present invention are not impaired, and other aesthetic satisfaction may be improved and specific properties may be imparted Additives such as pigments, fragrances, bactericides, antioxidants and sequestering agents may be added.

According to another aspect of the present invention, there is provided a soap produced by the method for producing soap.

According to the present invention, it is possible to produce a soap which is very beneficial for the beauty of the skin, as it has excellent stability and feeling against skin irritation and exerts antioxidative effects.

FIG. 1 shows a process for producing a perilla leaf powder according to an embodiment of the present invention.
FIG. 2 shows a process for producing a perilla leaf fermentation broth according to an embodiment of the present invention.
FIG. 3 shows a process for manufacturing a sesame leaf extract according to an embodiment of the present invention.
FIG. 4 illustrates a process for preparing a caustic soda liquid containing a leaf-leaf fermentation broth during a process of manufacturing a leaf-soap according to an embodiment of the present invention.
5 illustrates a process of manufacturing a sesame leaf soap according to an embodiment of the present invention.
FIG. 6 is a skin irritation questionnaire form of the present invention.
FIG. 7 shows an experimental procedure for verifying the antioxidant activity of the perilla leaf fermented liquid prepared according to one embodiment of the present invention.
FIG. 8 shows the results of a test for verifying the antioxidant activity of a perianth livestock fermented liquid prepared according to an embodiment of the present invention. A and A ': Vitamin C (positive control), B and B': Lactobacillus acidophilus and Bacillus subtilis fermented with perilla leaf, C and C ': Leaf leaf fermentation fermented with Saccharomyces cerevisiae , D and D ': Leaf-leaf fermentation broth fermented with Lactobacillus acidophilus, Bacillus subtilis and Saccharomyces cerevisiae.
FIG. 9 is a graph showing the results of an antioxidant activity test result of a perilla leaf fermentation liquid prepared according to an embodiment of the present invention. A: Vitamin C (positive control), B: Lactobacillus acidophilus and Bacillus subtilis fermented with leaves, C: Leaf leaf fermentation fermented with saccharomyces cerevisiae, D: Lactobacillus ashidophilus , Bacillus subtilis, and Saccharomyces cerevisiae.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1. Preparation of sesame leaf powder

The sesame leaves were collected directly from Jiri Mountain.

① The collected leaves were washed 2 ~ 3 times with clean water, transferred to a water dropping bowl and drained for 30 ~ 60 minutes.

② The leaves were dried in a food drier at 50 ℃ for 6 ~ 8 hours or lyophilized.

③ Dried sesame leaf was put into a blender and ground for 1 minute, followed by 1 minute of resting. Repeated to make fine powder, sesame leaf powder was prepared.

④ Leaf leaf powder was put in a zipper bag or a container that can block air completely and kept in a freezer.

Example 2: Preparation of sesame leaf fermentation broth

Using the perilla leaf powder of Example 1, the perilla leaf fermentation broth was prepared as follows.

Composition of medium for fermentation of leaves: 1 liter of distilled water (or bottled water), 20 g of sugar, 5 g of sesame leaf powder

① 700 ~ 800 ml of distilled water, 20g of sugar and 5g of sesame leaf powder were weighed into a 2L glass beaker and stirred to completely dissolve the sugar and mix the sesame leaf powder evenly.

② The mixture was transferred to a 1 liter mass cylinder and filled with distilled water so that the total volume was 1 liter, and a medium for fermentation of sesame leaves was prepared.

③ In a 2 liter Erlenmeyer flask, a medium for fermentation of leaves was placed, and Lactobacillus acidophilus ATCC 4355, Bacillus subtilis KCTC 27134, and Saccharomyces cerevisiae KCTC 27209 10 ml of the culture solution was inoculated.

④ After that, the cells were cultured in a shaking incubator at 35 ° C and 120 rpm for 5 days.

⑤ The culture broth was transferred to a tube and centrifuged at 4 ° C and 5,000 rpm for 10 minutes.

(6) The supernatant obtained by centrifugation was filtered with a filter paper to obtain a final leaf-leaf fermentation broth.

⑦ The sesame leaf fermentation broth was used in refrigeration.

Example 3: Preparation of sesame leaf extract

The manufacturing process of sesame leaf extract is as follows.

② The leaves were dried in a food drier at 50 ℃ for 6 ~ 8 hours.

(3) 100 g of dried sesame leaf was added with 1 liter of 70% (v / v) ethanol and extracted for 12 hours at room temperature or with stirring (repeated twice).

(4) After sonication at 30 ° C for 15 minutes, primary filtration with cotton balls was carried out to obtain a filtrate.

⑤ The primary filtrate was centrifuged and the supernatant was recovered.

(6) The supernatant was secondly filtered through a filter paper to obtain a filtrate.

⑦ The secondary filtrate was concentrated under reduced pressure and lyophilized to obtain a final powdered safflower extract.

⑧ The sesame leaf extract was put into a zipper bag or a container capable of completely blocking air and stored in a freezer.

Example 4. Preparation of sesame leaf soap

Soaps were prepared in the following manner according to the raw materials and the amounts of the soaps shown in Table 1 below.

Perfumed soap raw materials and usage Oil name Oil amount Award layer NaOH additive Palm 200 g 50% sesame leaf fermentation broth
(247.5 g)
106.5 g 5 g of sesame leaf powder (or 1 g of sesame leaf extract)
Fine FO (pine needles) 7 g coconut 200 g Soybean 150 g Rice bran 200 g

① All containers and tools used were sterilized with 70% ethanol and dried.

② Weigh 750g of oil weighed in a 2L stainless steel beaker and heat it at 50 ° C on a hot plate.

(3) 247.5g of 50% sesame leaf fermentation broth mixed with sesame leaf fermentation broth and water at a ratio of 1: 1 was weighed and placed in a 1L stainless steel beaker. Then, 106.5g of NaOH was added and dissolved, and the temperature was adjusted to 50 ° C.

④ The oil prepared in ② above and the mixture prepared in ③ are combined and stirred with a blender. When trace occurs, it is poured into a mold and kept in a warming oven for 24 hours.

⑤ At the end of warming, it was cut into a certain shape and dried naturally for 6 weeks.

Experimental Example 1. Evaluation of skin stability

The skin stability of the sesame leaf soap prepared in Example 4 was surveyed on 20 men and women aged 30 to 60 years.

As a result, there was subjective opinion that there was some itching, skin pulling or tightening stimulus as shown in Table 2 below, but there was no problem about skin irritation in general.

Clinical Observation Sesame oil soap No stimulation weakness Strong Very strong week
tube
enemy
character
drama Itching (pruritus) 18 (90%) 1 (5%) 1 (5%) 0 Stinginess 20 (100%) 0 0 0 Burning (burning sensation) 20 (100%) 0 0 0 Bitter 20 (100%) 0 0 0 ache 20 (100%) 0 0 0 Pulling / Fastening 17 (85%) 2 (10%) 1 (5%) 0
People
tube
enemy
character
drama Redness (erythema) 20 (100%) 0 0 0 Swelling (swelling) 20 (100%) 0 0 0 Milk sprouts 20 (100%) 0 0 0 Hardening (hardening) 20 (100%) 0 0 0 pimple 20 (100%) 0 0 0 acne 20 (100%) 0 0 0 Keratinized 20 (100%) 0 0 0 Blister or blister 20 (100%) 0 0 0 Pigmentation 20 (100%) 0 0 0

Experimental Example 5. Verification of antioxidant activity of sesame leaf fermentation broth

To examine the antioxidative activity of the perianth livestock fermented liquid prepared in Example 2, antioxidative activities of fermented livestock fermented according to kinds of useful microorganisms were measured and compared. Experimental methods and results were as follows. The fermentation was carried out in the same manner as in Example 2 except that the kinds of microorganisms differed.

(Scavenging capacity) of DPPH (2,2-diphenyl-1-picrylhydrazyl), which is a method for measuring the ability to eliminate free radicals causing skin aging.

(1) 0.5 ml of a sample (0.5 ml / 10 ml) dissolved in ethanol (or distilled water) was added to 1 ml of an ethanol solution of 0.2 mM concentration of DPPH.

② Leave at 24 ℃ for 30 minutes.

(3) The absorbance at 517 nm was measured to determine the concentration of eroded DPPH.

④ The free radical scavenging activity was calculated by the following equation.

% scavenging = [1- (B-C) / A] x 100 (%)

A: Absorbance after reaction without addition of sample

B: Absorbance after reaction with addition of sample

C: absorbance measured by replacing with DPPH by distilled water

⑤ As a positive control sample, vitamin C (ascorbic acid) prepared at 10 mg / ml was used.

As a result of confirming the antioxidative activity in the same manner as above, when the fermentation was performed using all three microorganisms as shown in Figs. 8 and 9, the antioxidant activity was remarkably excellent.

Claims

a) fermenting a sesame leaf with Lactobacillus acidophilus , Bacillus subtilis and Saccharomyces cerevisiae to obtain a leaf-leaf fermentation product; And
and b) adding the fermented product of the sesame leaf to produce a soap.

The method according to claim 1,
The step a)
a-1) pulverizing the sesame leaf to prepare a sesame leaf powder;
a-2) 200 to 600 parts by weight of sugar and 10,000 to 20,000 parts by weight of water are mixed with 100 parts by weight of the sesame leaf powder to prepare a medium for fermentation of leaves;
a-3) Lactobacillus acidophilus , Bacillus subtilis and Saccharomyces cerevisiae were inoculated into the medium for fermentation of the leafy leaves and cultured at 30 to 40 DEG C for 1 And fermenting the fermented product for 10 days to produce a perilla leaf fermented product.

The method according to claim 1,
The step b)
Wherein the sesame leaf fermented product is added to 1 to 12 wt% of the total weight of the soap composition to produce a soap.

The method according to claim 1,
The step b)
A method for producing a soap, comprising adding a sesame leaf powder or a sesame leaf extract to produce a soap.

5. The method of claim 4,
Wherein the sesame leaf powder is added in an amount of 0.1 to 1% by weight based on the total weight of the soap composition to produce a soap.

5. The method of claim 4,
The safflower extract is an extract obtained by extracting a sesame leaf with 50 to 90% (v / v) ethanol,
Wherein the soap is prepared by adding 0.01 to 0.1% by weight of the total weight of the soap composition based on the dry weight of the extract.

A soap produced by the method of any one of claims 1 to 6.