KR101793918B1 - Sports beverage composition for improving exercise ability and enhancing immune function comprising polysaccharide from Astragalus membranaceus as effective component - Google Patents

Abstract

The present invention relates to a sports drink composition for boosting immune functions and improving motor ability, containing milk vetch root-derived polysaccharide as an active ingredient. More specifically, by extracting polysaccharide from an aerial part and an underground part of milk vetch root respectively, mixing the same at the weight ratio of 1:1, and then orally administrating the same in a mouse model, immune functions reduced after workout become enhanced, endurance increases, and a rapid drop in blood glucose due to workout can be remarkably prevented, thereby being useful as sports drink compositions.

Description

[TECHNICAL FIELD] The present invention relates to a sports beverage composition for improving exercise ability and immune function, comprising a polysaccharide derived from hwanggi as an active ingredient,

The present invention relates to a composition for improving exercise performance and enhancing immune function, which comprises a polysaccharide derived from a hwanggi as an active ingredient.

The amount of beverages consumed by a Korean citizen per year is about 46 per 1.5L PET bottles. The consumed beverage was mainly carbonated beverage, and other juice beverage was found to be main. On the other hand, other beverages in niche markets, which are surely palatable, had a dramatic market expansion of about 43% in 2001 compared to the previous year, and their growth rate is much faster than the carbonated drinks and juice market. Of the market. In particular, functional beverages belonging to other beverages have been increasing in market in accordance with international events and marketing, and have recently become a living beverage with growing interest in living sports. In functional beverages, sports drinks prevent sweating, work in heavy or hot environments, prevent dehydration, maintain electrolyte (ion) balance of body fluids, and inhibit a significant reduction in carbohydrate storage in the body And is an electrolyte supplementary drink for supplying water or an electrolyte in a situation where sweat is excessively flowed.

Most of the commercially available sports drinks contain about 6 to 8% (w / v) of various kinds of saccharides for energy supplementation, and the concentration of saccharides contained in the sports drinks is 8% (w / v), there is a report that the intestinal absorption rate of beverage is delayed. In addition, the sports beverage contains electrolytes such as sodium, potassium, magnesium, and calcium for electrolyte balance by ion replenishment, and may contain some vitamin C or the like. In addition, the functional beverage including the sports beverage may be supplemented with a coloring agent such as blue, yellow, and red for a visual refreshing sensation. Studies on the above-mentioned functional beverages, in particular, sports beverages have been mainly conducted on the production of highly functional sports beverages in which a high functional role such as muscle formation and mobility improvement is added in addition to basic functions of water and electrolyte diffusion of existing ionic drinks . Such highly functional sports drinks may further contain alginic acid, carnitine, taurine, or branched amino acids in addition to saccharides and electrolytes depending on the characteristics and uses of the product. More specifically, recently developed sports drinks have various functions such as fatigue recovery or endurance enhancement at a stage of satisfying only thirst relief. That is, the high-functional sports beverage is useful as an energy source in a situation where a large amount of rapid energy supply is required before, during, or after exercise, and is capable of supplying electrolytes and moisture in a rapidly lost body due to excessive sweat generation . Accordingly, the highly functional sports beverage may be one containing an electrolyte as a main component and containing sweeteners such as glucose, fructose, and oligosaccharides, water-soluble vitamins, amino acids, and the like.

In this regard, Ergogenic Aids, a food that has recently been created to quickly and easily obtain nutrients and energy efficiently at sporting sites, is being used as an adjuvant or adjuvant to improve performance. However, in the case of the above-mentioned enhancing adjuvant, there is a problem in relation to a muscle enhancer such as a protein supplement, a carbohydrate supplements or anabolic steroid, so that a high-performance sports drink derived from a natural product Development is required.

Korean Patent No. 0364000 discloses a sports beverage composition and a manufacturing method thereof, and Korean Patent No. 0714464 discloses a sports functional food. However, a sports beverage composition for improving exercise performance and for improving immune function, which contains the polysaccharide derived from the herbaceous period of the present invention as an active ingredient, has not yet been disclosed.

The present invention has been made in view of the above-mentioned needs. In the present invention, polysaccharides are extracted from the ground and underground parts of the hull, and mixed with each other at a weight ratio of 1: 1. The present invention has been accomplished by confirming that it exhibits an effect of not only enhancing immune function lowered after exercise and improving endurance but also remarkably suppressing rapid blood sugar reduction due to exercise.

In order to achieve the foregoing object, the present invention Astragali (Astragalus The present invention provides a sports beverage composition for improving exercise performance and enhancing immune function, comprising a polysaccharide derived from P. membranaceus as an active ingredient.

The present invention also provides a sports drink for improving exercise performance and improving immune function, which comprises the beverage composition.

In the present invention, polysaccharides are extracted from the upper part of the hull and the lower part thereof, mixed at a weight ratio of 1: 1, and then orally administered to an animal model mouse. As a result, the immune function after exercise is lowered and the endurance is improved. And thus can be usefully used as a sports beverage composition.

FIG. 1 shows the effect of swimming marginal exercise on oral administration of polysaccharide (AMA) extracted from the upper part of the hull, a polysaccharide (AMR) extracted from the lower part of the hull and a mixture thereof (AMA0.5 + AMR0.5). AMR is a group of oral administration of polysaccharides extracted from the basal part of the Yellow Ridge, and AMA0.5 + AMR0.5 is an experimental group of AMA0.5 + AMR0.5. The control group is a control group of oral administration of sterilized physiological saline, Mixed polysaccharide extracted from the above ground part and polysaccharide extracted from the lower part of the shiitake. * ≪ / RTI > means a statistically significant increase in swimming time limit of the oral administration group of erysipelas polysaccharide compared to the negative control group to which nothing was administered.
FIG. 2 shows the effect of suppressing rapid blood glucose decrease due to exercise when the polysaccharide (AMA) extracted from the upper part of the hull, the polysaccharide (AMR) extracted from the lower part of the hull and the mixture thereof (AMA0.5 + AMR0.5) The result is confirmed.

In order to achieve the object of the present invention, the present invention provides a sports beverage composition for improving athletic performance and improving immune function, comprising a polysaccharide derived from Astragalus membranaceus as an active ingredient.

In a sports beverage composition according to an embodiment of the present invention, the immune function enhancement may be to promote decreased immune function after exercise, but is not limited thereto.

The polysaccharide may be a polysaccharide extracted from the lower part of the stem, a polysaccharide extracted from the lower part of the stem, or a mixture thereof. Preferably, By weight, more preferably 1: 1 by weight, but the present invention is not limited thereto.

The polysaccharide may be at least one selected from sweeteners, flavors, minerals, vitamins, preservatives, emulsifiers, stabilizers, acidulants and thickeners in addition to the polysaccharides derived from the erythrocytes.

The sweetener may be used in an amount that sweetens the beverage to a suitable sweet taste, and may be natural or synthetic. Natural sweeteners include sugar sweeteners such as corn syrup solids, honey, xylitol, sucrose, fructose, lactose, maltose, and glucose.

The flavoring agent may be used to improve taste or aroma, and both natural and synthetic flavoring agents may be used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. The natural flavorings may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, etc., or obtained from green tea leaves, Aspergillus, Daegu, Cinnamon, Chrysanthemum leaves, Jasmine and the like. In addition, ginseng (red ginseng), bamboo shoots, aloe vera, obtained from the bank may be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavoring agents may be used depending on the case. Synthetic flavoring agents such as esters, alcohols, aldehydes and terpenes may be used.

The minerals may be calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, .

The vitamins include various water-soluble and lipid-soluble vitamins such as vitamin A (retinol), vitamin B1 (thiamine), vitamin B2 (riboflavin), vitamin B3 (niacin), vitamin B6 (pyridoxine), vitamin B12 Vitamin C (ascorbic acid), vitamin D (cholecalciferol, etc.), vitamin E (tocopherol), bisbenthiamine, nicotinic acid amide, calcium pantothenate, folic acid, biotin and heavy tartaric acid choline have.

Examples of the preservative include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, and EDTA (ethylenediaminetetraacetic acid). Examples of the emulsifying agent include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like.

The stabilizer refers to a material that is dissolved in water to exhibit viscosity. The stabilizer may be used in combination with gum in the present invention. The stabilizer may impart viscosity to the beverage of the present invention and may also have an emulsion stabilizing effect. Examples of such stabilizers include, but are not limited to, pectin, carrageenan, gellan gum, locustbean gum, xanthan gum, agar, konjac, gum, arabic gum, pullulan gum, psyllium seed gum, guar gum, tamarind gum, tara gum, Furcellaran gum gelatin, sodium alginate, or a combination thereof.

The acidulant may include math, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid and citric acid. These acidulants may be added so that the beverage composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

The thickening agent may be a suspending agent, a sedimentation agent, a gel-forming agent, a swelling agent, and the like.

The present invention also provides a sports drink for improving exercise performance and improving immune function, which comprises the beverage composition.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Materials and methods

1. Separation of Polysaccharides from Above and Below

In order to separate the active polysaccharides from the upper and lower parts of the shorelines, 1 kg of distilled water having a volume of 20 times was added to each of the ground and underground portions of 1 kg, and the mixture was pulverized and stirred. The mixture was heated to half of the distilled water. . The filtrate was concentrated under reduced pressure, concentrated by adding 5-fold amount of ethanol and precipitated. The remaining precipitate was centrifuged at 7,000 rpm for 30 minutes at 4 ° C to obtain supernatant And the residue was separated. The supernatant was again lyophilized to obtain a hot-water extract fraction.

In order to confirm the above-ground and Astragalus Polysaccharide configured per the below-ground, if the neutral sugar with respect to the obtained sample, after hydrolysis of the sample for 1.5 h at 121 ℃ in a 2.0M TFA (trifluoroacetic acid) with NaBH ₄ to the neutral sugars Reduction to alditol and conversion to alditol acetate derivatives using (CH ₃ CO) ₂ O was performed using SP-2380 column (0.2 μm film, 0.25 mm id × 30 m, Supelco, Bellefonte, PA , USA) were analyzed by GLC (HP-6890 II GLC, Hewlett-Packard, Palo Alto, CA, USA). In the case of acidic saccharides, GLC was carried out by converting sodium alginate to an alditol acetate derivative in the same conditions as in the case of neutral sugars by using NaBD ₄ after hydrolysis with 0.1 M TFA at 100 ° C for 1 hour . The temperature condition of the GLC for the constitutional analysis was 250 ° C. for the injector and 250 ° C. for the detector. The column was elevated to 220 ° C. at a rate of 30 ° C./min after 1 minute at 60 ° C., The temperature was raised to 250 ° C at a rate of 8 ° C / min to maintain the temperature for 15 minutes. The constituent sugar in the sample was analyzed by comparing with the retention time of the standard saccharide, and the molar percentage (mol% peaks were calculated from molar ratios calculated from the area ratio of alditol acetate derivatives and the molecular weight of the alditol acetate derivative.

2. In experimental animals Immunity  black

(1) Oral administration of hyperpigmented polysaccharides and polysaccharides Lymphocyte  detach

On May 22, 2014, a 6-week-old male SD (Sprague-Dawley) mouse was obtained from Korea BioLink and maintained at a temperature of 23 ± 3 ° C and a relative humidity of 50 ± 20% And were raised in an automatic contrast cycle system. (E) and non-exercise (NE) groups, and each group was divided into the negative control group (C), the sham group (AMA) group (AMA0.5 + AMR0.5) and sports drink treatment group (SPD). The treatment group (10 treatments) was divided into two groups. Each group was treated with sterile normal saline (1.5 ml) as negative control (C). The group treated with polysaccharide (AMA) in the horseradish peroxidase group showed hemolytic acitivity when administered orally to mice (AMR) was administered at a concentration of 10 mg / ml, which is the minimum level of immunity-enhancing effect when administered orally, and the dose of the administered solution was 10 ml / kg , The mice were orally administered two to three times a day for 30 days, and lymphocytes were separated from the test animals. The positive control group was compared with the SPD (Sports Drink) group (SPD), and the sports ionic beverage was prepared in accordance with the amount of liquid administered as it was in a liquid state.

Separation of lymphocytes from each treatment group was carried out in a sterile state by adding HBSS (Hank's balanced salt solution, Sigma) to extract the spleen of a mouse and passing through a steel mesh to obtain a homogenized cell supernatant. RBC dissolution buffer (red blood cell lysis buffer). The cell suspension was centrifuged (380 × g, 4 ° C. and 10 minutes), and the formed pellet (pellet) was treated with ammonium chloride (ammonium chloride 0.8%, w / v) to decompose the erythrocyte. ) Was washed three times with PBS and then diluted with DMEM (Dulbecco's modified Eagle's medium, Gibco Co, USA) supplemented with 1% penicillin-streptomycin (Gibco Co, USA) and 10% FBS , USA) at 37 ° C and 5% CO ₂ , respectively.

(2) Performing exercises

The animals were allowed to move for 15 minutes at 15m / min for 7 days using a treadmill (PBC47700 treadmill device; Coulborn Instruments Co., USA) It is possible to attach the impact device to motivate the movement. Limited exercise test was performed up to exercise devoting 30m / min to tilt the treadmill the day to 10 ℃ (70 ~ 75%, the maximum O ₂ consumption of maximum O ₂ consumption of animals is not able to maintain longer on a treadmill Of oxygen consumption).

(3) CD4 ⁺ / CD8 ⁺ Measurement of

Phycoerythrine anti-mouse CD4 ⁺ (0.2 μg / l × 10 ⁵ cells; PharMingen) was added to spleen lymphocytes suspended at a level of 2 × 10 ⁶ cells per 100 ml of PBS (phosphate buffer saline) containing 10% FBS , ⁵ μl of biotin anti-mouse CD8 ⁺ (0.2 μg / ℓ × 10 ⁵ cells; PharMingen) was added to each well and incubated at 4 ° C. for 30 minutes. Streptavidin-fluorescein isothiocyanate (Becton Dickinson) or streptavidin phycoerythrin were added. The cultured lymphocytes were further centrifuged at 300 × g for 3 min in PBS supplemented with 10% FBS. The stained lymphocytes were fixed with 2% paraformaldehyde, and a subset of lymphocytes stained (FACScan; Becton Dickinson, Franklin Lakes, NJ, USA).

3. Assessment of global athletic performance

On April 15, 2014, BioRink was purchased with 80 ICR male mice aged 4 weeks and adapted to the breeding environment for 7 days. Nonexercise control (NC), nonexercise (Nonexercise) (Nonexercise AMA 0.5 and AMR 0.5, NE-AMA 0.5 + AMR 0.5) and control group in the control group (nonexercise AMR, NE-AMR) (Exercise AMA, E-AMA), Exercise control (EC), Exercise AMA, E-AMR and E-AMR. AMA0.5 + AMR0.5). The dose of AIN-76A (Research diet, USA) was fed to the mouse, and the amount of 1 g / kg / day for the polysaccharide-treated polysaccharide and the polysaccharide-treated polysaccharide And adjusted to 0.5 g / kg / day so as to be the same amount as the single treatment. The doses were administered orally at the same time each day and the control group was orally administered the same volume of distilled water. On the last day of the experiment, the diet was removed 3 hours before the oral administration and marginal swimming exercise test was carried out. Blood was collected from the vena cava immediately after the exercise test and centrifuged at 3,000 rpm to separate the serum. Serum was immediately stored in a -70 ° C freezer and used for the experiment.

(90 × 45 × 45cm) was used to evaluate the global exercise ability according to the mixed administration of polysaccharide in the upper part of the upper part of the stem and the lower part of the stem, water was filled up to 35 cm in measuring the exercise ability, and the water temperature was maintained at 34 ° C. Respectively. The surface velocity was controlled by water circulation using a pump and a water flow meter (type F45500, Blue White Co, Westminster, Calif., USA) to which a voltage regulator was connected. In order to adapt to swimming during the 1 week preliminary breeding period before the experiment, 2 (7 L / min, 15 min) adaptive swimming was performed, and 2 global exercise capacities were measured, The time was the same, and the control group, the Shijiazhuang terrestrial polysaccharide, the underground polysaccharide, and the Shiga terrestrial polysaccharide were mixed with the ground polysaccharide. The subjects were fasted 3 hours before the exercise and 2 hours before the exercise. All swimming ability was measured from 1 pm to 5 pm and the global athletic ability was measured from the starting point of the mouse swimming to the threshold point Was measured.

4. Blood glucose measurement of experimental animals during marginal swimming exercise

Glucose was measured by tail vein blood during marginal swimming exercise. Blood glucose was measured using a blood glucose meter (ARKRAY, Kyoto, Japan) before the marginal swimming exercise, 15 minutes before the exercise, the threshold exercise, 15 minutes after the exercise, and 60 minutes after the exercise.

Example  1. The polysaccharide-derived polysaccharide-treated CD4 ⁺ And CD8 ⁺ Cell number  With increasing CD4 ⁺ / CD8 ⁺ Ratio maintenance effect

In Example 1, the number of CD4 ⁺ and CD8 ⁺ cells in the spleen after the marginal exercise was measured and compared with the existing sports drinks used as the positive control group in the experimental animals receiving the aerial part and the underground polysaccharide. As a result, as shown in Table 1 below, the CD4 ⁺ level was reduced to 19.3% in the negative control group (NE-C) while it decreased to 16.1% in the negative control group (EC) , And CD8 ⁺ , 15.1% in the non - exercise group and 8.8% in the exercise group in the negative control group. On the other hand, the number of CD4 ⁺ and CD8 ⁺ cells was increased in both the exercise and non-exercise groups in the case of the administration of the polysaccharide-containing polysaccharide in the hwanggi group, the polysaccharide group in the hwanggi- ) Maintained high levels of CD8 ⁺ cells that were rapidly decreased after exercise. For the CD4 ⁺ / CD8 ⁺ ratio, the ratio of unexcited at the negative control (NE-C) was about 1.33, while it increased to 1.86 after exercise in the same group. On the other hand, the negative control group (EC) showed a rapid decrease in immunity while the ratio of CD4 ⁺ / CD8 ⁺ in the non-exercise series was higher in the case of the group administered with the hyperkeratotic polysaccharide, the group with the basal polysaccharide and the group with the basal polysaccharide (CD4 ^{+ /} CD8 ^+), which was increased after exercise, by promoting differentiation into cytotoxic T lymphocyte (CD8 ⁺ ) and increasing the number of CD8 ⁺ cells after emptying and after exercise, showing 1.04, 1.06, 1.18 and 1.19 after exercise ⁺ Ratio to keep the change constant.

Measurement of CD4 ⁺ and CD8 ⁺ cell numbers and CD4 ⁺ / CD8 ⁺ ratio by the treatment of Shijiazhuang and underground polysaccharides Processing group CD4 ⁺ (%) CD8 ⁺ (%) CD4 ⁺ / CD8 ⁺ Before exercise NE-C 19.3 ± 2.1 ^a 15.1 ± 0.6 ^a 1.33 ± 0.11 ^c NE-AMA 20.9 ± 1.7 ^ab 19.4 ± 0.6 ^b 1.06 + 0.15 ^a NE-AMR 23.6 ± 1.3 ^b 19.9 ± 0.5 ^b 1.18 ± 0.19 ^ab NE-AMA0.5 + AMR0.5 24.2 ± 1.3 ^c 20.2 ± 0.6 ^b 1.19 ± 0.19 ^b NE-SPD 18.8 ± 1.1 ^a 14.6 ± 1.3 ^a 1.29 + - 0.13 ^b After exercise E-C 16.1 ± 0.8 ^a 8.8 ± 0.4 ^a 1.86 ± 0.09 ^c E-AMA 23.6 ± 0.3 ^b 21.5 ± 1.1 ^c 1.14 ± 0.12 ^a E-AMR 25.5 ± 0.8 ^c 24.6 ± 0.5 ^d 1.08 ± 0.10 ^a E-AMA0.5 + AMR0.5 27.4 ± 0.5 ^d 26.7 ± 0.8 ^e 1.05 + 0.14 ^a E-SPD 15.6 + 0.4 ^a 12.1 ± 0.8 ^b 1.34 + 0.04 ^b

NE-C was an experimental group in which non-exercise-type mice were administered sterile saline; NE-AMA is an experimental group in which non-exercise group mice were treated with erbotropic topical polysaccharide; NE-AMR is an experimental group in which a non-exercise-type mouse was administered with a basal polysaccharide of the Hwanggi group; NE-AMA0.5 + AMR0.5 was an experimental group in which the non-exercise group mice were mixed with the basal polysaccharide and the basal polysaccharide; NE-SPD is an experimental group in which non-exercise-type mice were given a sports drink (Gatorade); EC was an experimental group in which exercise-type mice were administered sterile physiological saline; E-AMA is an experimental group in which exercise-type mice were treated with a hyperkeratotic polysaccharide; E-AMR is an experimental group in which exercise-type mice were administered a basal polysaccharide of Hwanggi group; E-AMA0.5 + AMR0.5 was an experimental group in which exercise-type mice were mixed with a basidiomycetous polysaccharide and a basidiomycetes; E-SPD is an experimental group that sports drinks (Gatorade) were administered to exercise-type mice.

Example  2. Effect of the present invention on the effect of polysaccharide treatment on the global swimming ability

The mouse was administered with a mixture of a hyperkeratotic polysaccharide, a polysaccharide of the underground polysaccharide and a polysaccharide of the underground polysaccharide and a polysaccharide of the underground polysaccharide, and then exercised under the exercise intensity of the flow rate of 7 L / min, which is the anaerobic threshold, The exercise time was measured. As a result, as shown in FIG. 1, the ability to improve the performance during the experimental period was 52% and 68% (22.7 minutes and 29.8 minutes) on the 21st and 28th days of exercise on the AMR of the basal polysaccharide group, And 61% and 64% (18.4 minutes and 20.8 minutes), respectively. In addition, the administration of polysaccharide in the upper part of the shrub and lower part of the body had a significant effect on the improvement of the athletic performance. On the other hand, in the group administered with the same dosage amount (AMR0.5 + AMA0.5) mixed with the lower part of the basal area, And underground polysaccharide mixed group showed 75% of the ability to improve the athletic performance. Therefore, it is considered that the long-term application of the mixture of the topical and the basal polysaccharide of the hwanggi group will show a more effective exercise capacity improvement effect.

Example  3. Effect of the present invention on blood sugar-holding effect according to polysaccharide treatment

At rest, muscles use free fatty acids as their primary source of energy, but they use glucose mainly when exercise begins. Therefore, the muscles in motion use glucose and blood glucose produced through decomposition of the glycans in muscle as necessary energy sources. Therefore, the amount of glucose in the blood during exercise decreases. The level of glucose, which is decreased at the limit of time, consumes more glucose in the muscles as the exercise time becomes longer. As a result, the glycogen in the liver and muscles is actively decomposed with glucose.

As shown in FIG. 2, the concentration of glucose in the blood after the single administration and the mixed administration of the aerial part and the underground part of the hull was measured. In the control group, rapid decrease in glucose concentration occurred in the blood from the exercise time limit to 15 minutes after the exercise (AMR) and hyperglycosylated polysaccharide (AMA) group. However, the decrease of glucose concentration in the exercise time limit was about 50% lower than that of the control group. In addition, it was confirmed that in the case of the mixed administration group (AMA0.5 + AMR0.5) of the aerial part and the underground part, rapid decrease of glucose concentration in the blood was remarkably reduced from the exercise limit time to the resting time of 15 minutes after exercise.

Example  4. Sensory evaluation of prototype sports drinks containing the polysaccharide of the present invention

In Example 4, a combination of a shiitake polysaccharide and a shiitake polysaccharide having reduced immune function after exercise were mixed and used in the production of a prototype. This study was carried out to obtain the aerobic polysaccharide and the polysaccharide of underground polysaccharide which were used in the evaluation of efficacy of immune function improvement after exercise. . In order to improve the nutritional and sensory aspects of beverage prototypes, carnitine, whey protein, beverage opacity was added to each concentrate as an additive. Effect, etc.), vitamin C, vitamin B (niacin), apple concentrate, xylitol, citric acid and glucose. In the beverage preparation, whey protein was added to purified water and homogenized (hydrated) for 20 minutes and heat-treated at 88 ° C for 2 minutes. Carnitine, apple juice concentrate, hwanggi topical polysaccharide, basal polysaccharide, vitamin B (niacin), xylitol, citric acid, malic acid and glucose were mixed and heated to 95 ℃ and allowed to cool to 88 ℃. Vitamin C and citron were added, filled in a container and cooled in ice water. The pH was adjusted to 3-4. A total of 26 recipes were prepared through pre - tasting, and 3 of them were selected to produce beverages. The contents of each ingredient in the prepared beverage were as shown in Table 2 below and were made into a pale yellowish white opaque beverage and no large change in appearance between the prototypes was observed.

Components of prototype sports drinks containing hwanggi-derived polysaccharides g / 100g A B C Purified water 88.78 88.68 90.68 Whey protein
(whey protein) 0.03 0.03 0.03 Shingon 0.01 0.01 0.01 Hwanggi Basement Polysaccharide 0.04 0.04 0.04 Apple concentrate 0.60 0.60 0.60 Carnitine 0.01 0.01 0.01 glucose 4.00 4.00 3.00 Malic acid 0.10 0.10 0.10 Citric acid 0.02 0.02 0.02 Xylitol 6.00 6.00 5.00 Vitamin B 0.01 0.01 0.01 Vitamin C 0.40 0.40 0.40 Yuzu cotton 0.00 0.10 0.10 pH 3.43 3.51 3.51

The sports drinks containing hwanggi polysaccharide were prepared by three kinds of A, B and C and sensory tests were conducted. The sensory test was conducted by using the method of sensory test conducted in domestic and imported drinks and drinks. Sensory evaluation was performed on twenty - eight male and female twenty - eight selected for the product. For each sample, the appearance preference degree, incense degree degree, and overall degree of likelihood were evaluated using the 7-point symbol scale (1 point: very disliked, 4 points: neither good nor disliked and 7 points: very good) , Sourness degree, bitterness degree and posterior degree were evaluated by the 7 - point symbol scale, and the results were derived by means of the mean and standard deviation. As a result, as shown in the following Table 3, the score of B was the highest in appearance, color, flavor, sweet taste, sour taste, bitter taste, aesthetic taste, overall taste and overall taste, and appearance and bitterness A was the lowest in the items except for. There was no difference in the content of beverages, and B was the most suitable for making and using sports drinks when compared with preference survey.

Sensory Evaluation Preference Analysis of Sports Drink Prototype Containing Polysaccharide Derived from Hwanggi A B C Exterior 4.43 + - 1.230 4.64 ± 1.254 4.36 ± 1.311 color 4.14 + 1.044 4.61 ± 1.066 4.50 ± 1.036 incense 3.86 ± 0.970 5.75 + 1.236 5.00 ± 1.122 sweetness 4.14 ± 1.380 5.25 ± 1.143 4.75 ± 1.076 Sour taste 3.71 ± 1.410 5.43 ± 1.168 4.71 ± 1.150 bitter 4.21 ± 1.572 4.71 ± 1.512 4.11 + 1.343 cove 3.43 ± 1.106 4.89 ± 1.618 4.43 ± 1.399 Overall likelihood 3.68 ± 1.371 5.57 ± 1.425 4.96 ± 1.319

Claims (6)

1) Astragalus membranaceus : a step of adding 20 times volume of water to each of the above and below the ground, pulverizing, heating and extracting;
2) concentrating the extracts of the light yellow, ground and bottom part of the step 1) under reduced pressure, respectively, adding 5-fold amount of ethanol to each of the obtained concentrated sulfuric acid and the concentrate of the underground part to obtain respective precipitates; And
3) After step 2), each of the obtained precipitates is centrifuged to obtain a supernatant, and Astragalus membranaceus polysaccharides derived from the above ground and polysaccharides derived from the ground are mixed at the same weight ratio A sports beverage composition for improving exercise ability containing a mixture as an active ingredient. delete delete delete The sports beverage composition according to claim 1, further comprising at least one selected from sweeteners, flavors, minerals, vitamins, preservatives, emulsifiers, stabilizers, acidifiers and thickeners in addition to the polysaccharides derived from the erythrocyte. A sports drink for improving athletic performance characterized by comprising the sports beverage composition of claim 1 or 5.

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