KR101785968B1 - Cosmetic composition for improving skin condition - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a biomimetic composite as an active ingredient, and more particularly to a cosmetic composition comprising a phospholipid, a Urea, a Glucose, N-Acetyl Glucosamine and a mineral The present invention relates to a cosmetic composition which contains, as an active ingredient, a cosmetic composition containing a biomimetic composite comprising a water-soluble polymer and a mineral water and exhibits an antioxidant effect on the skin, an anti-inflammatory effect, a skin wrinkle-improving effect and a skin elasticity-improving effect.

Description

[0001] The present invention relates to a cosmetic composition for improving skin condition,

TECHNICAL FIELD The present invention relates to a cosmetic composition containing a biomimetic composite, and more particularly, to a cosmetic composition containing a biomimetic composite as an active ingredient and exhibiting antioxidant, anti-inflammation, skin wrinkle, skin elasticity improvement and skin moisturizing effect.

With the expansion of women's social advancement into modern society, beauty has become a desire and a competitiveness for women. Skin is the largest tissue that surrounds the surface of the human body. It is in direct contact with the outside and protects the human body from stimuli such as temperature, humidity, bacteria, pollutants, and ultraviolet rays. However, as we get older, the cells that make up the skin become damaged or lose function. Such skin aging is caused by genetic and environmental factors. Intrinsic aging is an aging phenomenon that occurs over time according to genetic information as natural aging, and extrinsic aging is an aging phenomenon caused by external environmental factors. As the aging progresses, collagen, which is a main component of the dermal layer, is degraded and the function of the fibroblast is lowered, so that the skin gradually loses its elasticity and wrinkles increase. Strong ultraviolet rays, stress, and hormone imbalance increase melanin synthesis, causing staining pigmentation. In order to inhibit such aging and to improve the skin condition, various studies on the development of functional cosmetics are under way.

Biomimetics is a combination of bio and mimetics, a technique for creating new things by mimicking the behavior or structure of living organisms or the substances they produce. Biomimetic technology is used in various fields such as medical adhesives, clothing, airplane materials, and the field is continuously expanding. In the field of cosmetics, it is also attempting to develop new cosmetic raw materials by combining such biomimetic technology.

The inventors of the present invention conducted studies to produce a complex mimicking amniotic fluid as a motif and found that a phospholipid, a Urea, a Glucose, an N-Acetyl Glucosamine ) And mineral water (mineral water) at a certain ratio showed excellent skin improving activity, thus completing the present invention.

Korean Patent Registration No. 10-0977672 (Aug. 18, 2010) Korean Patent Registration No. 10-1562368 (Oct. 14, 2015)

It is an object of the present invention to provide a cosmetic composition containing as an active ingredient a biocompatible composite prepared by imitating a part of a biocomponent.

It is also an object of the present invention to provide a cosmetic composition for antioxidation, skin inflammation improvement, skin moisturizing, skin wrinkle and elasticity improvement containing the biomimetic composite.

According to an aspect of the present invention, there is provided a method of manufacturing a bio-material comprising a phospholipid, a Urea, a Glucose, N-Acetyl Glucosamine and Mineral water, There is provided a cosmetic composition containing a mimetic composite. Wherein the biomimetic composite comprises 0.0001 to 10 parts by weight of a soybean-derived phospholipid, 0.0001 to 10 parts by weight of a urea, 0.0001 to 10 parts by weight of glucose, 0.0001 to 10 parts by weight of N-acetylglucosamine, And 0.001 to 100 parts by weight of mineral water. More preferably, the biomimetic complex comprises 0.1 to 1 part by weight of a phospholipid, 0.01 to 0.1 part by weight of urea, 0.01 to 0.1 part by weight of glucose, N-acetyl glucosamine (N-acetyl glucosamine) 0.1 to 1 part by weight and mineral water (90.0 to 97.0 parts by weight).

The soy-derived phospholipid may be at least one selected from the group consisting of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI).

The biomimetic composite is contained in an amount of 1 to 99% by weight, preferably 50 to 99% by weight based on the total weight of the cosmetic composition.

The cosmetic composition containing the biomimetic composite of the present invention can be used as a cosmetic for antioxidation, skin wrinkle improvement, skin elasticity improvement, skin irritation reduction, skin moisturizing.

The cosmetic composition may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritive cream, a moisturizing cream, a hand cream, A lotion, a cleansing cream, a body lotion, a body cleanser, an emulsion, a press powder, a loose powder, and an eye shadow.

The biomimetic complex of the present invention exhibits an excellent antioxidative activity, an anti-inflammatory activity, an inhibitory activity of elastase, and an effect of promoting collagen biosynthesis, and thus can be usefully used for the production of various functional cosmetics.

The present invention relates to a cosmetic composition containing a biomimetic complex comprising a phospholipid, Urea, Glucose, N-Acetyl Glucosamine and Mineral water, .

Amniotic fluid is the fluid in the amniotic membrane that surrounds the fetus, protecting the fetus. The fetus is surrounded by a thin film called the amnion, the amniotic membrane is filled with amniotic fluid, and the fetus grows in this amniotic fluid. Amniotic fluid is similar to that of seawater. In addition to not having the greatest risk of being in the water for a period of 10 months, amniotic fluid also contains transforming growth factor-β, a growth factor similar to EGF, and epidermal growth factor (EGF) Amniotic fluid promotes the differentiation and growth of cells to help digestive and respiratory development. In China, amniotic fluid has been conventionally used as a cosmetic. In recent years, studies have been conducted to extract active ingredients such as stem cells from amniotic fluid and use them as cosmetics.

The inventors of the present invention have conducted experiments using various components and found that the active ingredient is selected from phospholipid, Urea, Glucose, N-Acetyl Glucosamine and Mineral water And to find the optimum mixing ratio of these.

Among these components, soy phospholipid means a phospholipid extracted from soybean, which helps strengthen skin barrier. The soy-derived phospholipid may be at least one selected from the group consisting of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI), preferably phosphatidylcholine (PC).

The mineral water is a mineral water containing a small amount of minerals such as calcium, magnesium and potassium, and is also referred to as mineral water. There are seven essential minerals that play an important role in controlling and maintaining the various physiological functions of our bodies. These include calcium (Ca), potassium (K), magnesium (Mg), sodium (Na), chlorine (Cl), phosphorus (P) and sulfur (S). Minerals account for 4% of human constituents, but they are not produced in the body and should be ingested through water or food.

The mineral water used in the present invention is a mineral water containing 1 to 100 mg / l of calcium (Ca), 1 to 100 mg / l of potassium (K), 1 to 100 mg / l of magnesium (Mg) 1 to 100 mg / l of iron (Fe), 0.01 to 10 mg / l of zinc, 0.01 to 10 mg / l of manganese (Mn), 0.01 to 10 mg / l of germanium (Ge) Means water containing 0.01 to 10 mg / l of sulfur, 0.01 to 10 mg / l of sulfur (S), 0.0001 to 0.1 mg / l of silicon (Si) and 0.0001 to 0.1 mg / l of lithium (Li)

The cosmetic composition of the present invention comprises a biomimetic complex comprising a phospholipid, Urea, Glucose, N-Acetyl Glucosamine and Mineral water as an active ingredient . Wherein the biomimetic composite comprises 0.0001 to 10 parts by weight of a soybean-derived phospholipid, 0.0001 to 10 parts by weight of a urea, 0.0001 to 10 parts by weight of glucose, 0.0001 to 10 parts by weight of N-acetylglucosamine, And 0.001 to 100 parts by weight of mineral water. More preferably, the biomimetic complex comprises 0.1 to 1 part by weight of a phospholipid, 0.01 to 0.1 part by weight of urea, 0.01 to 0.1 part by weight of glucose, N-acetyl glucosamine (N-acetyl glucosamine) 0.1 to 1 part by weight and mineral water (90.0 to 97.0 parts by weight).

Most preferably, the phospholipid, Urea, Glucose, N-Acetyl Glucosamine and Mineral water are mixed in a ratio of 1: 0.1: 0.1: 1: 97 .

The biomimetic composite showed excellent anti-inflammatory effects (Test Example 1), antioxidant effect (Test Example 2), skin elasticity improvement effect (Test Example 3), and collagen synthesis enhancing effect (Test Example 4).

The biomimetic composite is contained in an amount of 1 to 99% by weight, preferably 50 to 99% by weight based on the total weight of the cosmetic composition. The biomimetic complex exhibits excellent skin improving effect and can be used alone or in combination with other active ingredients, so that it can be used together with an active ingredient having other skin improving activity. Since it can be used in place of water in the production of cosmetics, cosmetics having excellent skin improving activity can be produced in various formulations.

The cosmetic composition containing the biomimetic composite of the present invention can be used as a cosmetic for antioxidation, skin irritation, skin wrinkle improvement, skin elasticity improvement, and skin moisturizing.

The cosmetic composition may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritive cream, a moisturizing cream, a hand cream, A lotion, a cleansing cream, a body lotion, a body cleanser, an emulsion, a press powder, a loose powder, and an eye shadow.

[Example]

Hereinafter, the present invention will be described in more detail with reference to the following examples. It should be understood, however, that these embodiments are merely examples for the purpose of easily describing the scope and scope of the technical idea of the present invention, and thus the technical scope of the present invention is not limited or changed. It will be apparent to those of ordinary skill in the art that various changes and modifications can be made within the scope of the technical idea of the present invention based on these examples.

Example  1 to 6: Preparation of Biomimetic Composition

Biomimetic complexes comprising soy-derived phospholipids, Urea, Glucose, N-Acetyl Glucosamine and Mineral water were prepared in the proportions shown in Table 1 below .

N-Acetyl Glucosamine, and Mineral water (trade name: GOLDEN MOUNTAIN SPRING WATERS) are commercially available, and are commercially available under the trade name of Phospholipid (product name: Hydrogenated Lecithin), Urea, Glucose, N-Acetyl Glucosamine and Mineral water Were purchased and used.

Component (% by weight) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Phospholipids 1.0 1.0 1.0 1.0 1.0 1.0 Urea 0.1 0.1 0.01 0.01 0.1 0.1 Glucose 0.1 0.1 0.01 0.1 0.1 0.1 N-acetylglucosamine 1.0 1.0 1.0 1.0 1.0 1.0 Mineral water 97 90 95 95 80 60 Purified water Balance Balance Balance Balance Balance Balance

Comparative Example  1 to 4: Preparation of Comparative Composites

Comparative complexes were prepared by mixing soy-derived phospholipids, Urea, Glucose, N-Acetyl Glucosamine and Mineral water as shown in Table 2 below.

Component (% by weight) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Phospholipids - 1.0 1.0 1.0 Urea 0.1 - 0.1 0.1 Glucose 0.1 0.1 - 0.1 N-acetylglucosamine 1.0 1.0 1.0 - Mineral water 97 90 90 90 Purified water Balance Balance Balance Balance

Test Example  1: cell activity test

Cell viability assay was performed to measure the survival rate of RAW264.7 cells according to the concentration of the biomimetic complex of Example 1 above. 100 μL (10,000 cells / well) of cell suspension was dispensed into a 96-well plate, preincubated for 4 hours in a CO ₂ incubator, and then incubated with 0 (0.1% DMSO) Biomimetic complexes at concentrations of 200, 300, 400 and 500 μg / mL, LPS at a concentration of 2 μg / mL were incubated with DMEM medium containing 10% FBS for 20 hours. After incubation, 10 μL of CCK-8 solution (Dojindo, Tokyo, Japan) was added to each well. After incubation for 2 hours in a CO2 incubator, absorbance was measured at 450 nm using a microplate reader.

The results are shown in Table 3 below.

Concentration (μg / mL) Example 1 0 100.0 50 98.03567 100 105.6732 200 109.4985 300 115.171 400 126.238 500 135.8623

Test Example  2: Measurement of anti-inflammatory effect of biomimetic complex

In order to examine the anti-inflammatory activity of the biomimetic complexes of the above Examples and Comparative Examples, the effect on TNF-α production, one of the early inflammatory molecules, was examined. The formation of pro-inflammatory cytokines such as TNF-a leads to the process of converting arachidonic acid into prostaglandin (prostaglandin) and NO formation due to the activity of phospholipase A2 (phospholipase A2) .

The cells used were HaCaT (Human Skin Keratinocyte cell line), Dulbecco's modified Eagle's medium supplemented with 5% CO2, 10% fetal bovine serum (FBS, Lonza) and 50 μg / mL streptomycin (DMEM, Invitrogen). After incubation, the cells were stressed by UV irradiation for 1 hour. As a control, UV-irradiated cells were used without sample treatment.

For RNA analysis, total RNA in cells was extracted from cell culture using Trizol reagent (Invitrogen, USA). The purity and integrity of the RNA was confirmed by A260 nm / A280 nm ratio measurement, and the RNA yield was measured by absorbance at 260 nm.

For cDNA synthesis, 3 μg of total RNA was added at 25 ° C. for 10 minutes with addition of Oligo dT 15 (500 ng / μl) primer, dNTP (10 mM), RTase inhibitor (40 U / μl) and Powerscript II RTase (Clontech, USA) The cDNA was synthesized by primer annealing at 42 ° C for 60 min and RTase denaturation at 95 ° C for 5 min. To amplify TNF-α and GAPDH from cDNA, 3 μl of cDNA, 5 μl of 10 × taq polymerase buffer, 2 μl of 10 mM dNTP, 2 μl of each 10 pmol primer and 0.5 μl of taq polymerase were mixed and adjusted to 50 μl by adding distilled water Lt; / RTI > The primer sequences are shown in Table 4. The PCR product was electrophoresed on a 1% agarose gel and analyzed with an image analyzer (UGEN, U: Genius). The density of each band was measured using a density measurement program (Gene Tools from Syngene) Respectively.

Gene Primer GAPDH
Forward 5'- AAC GAA TTT GGT CGA ACA GC-3 ' Reverse 5'-TGA GGA GGG ATT CAG TG-3 ' TNF-a
Forward 5'-CAG AGG GAA GAG TTC CCC AG-3 ' Reverse 5'-CCT TGG TCT GGT AGG AGA CG-3 '

The results are shown in Table 5 below.

TNF-α expression (%) UV irradiation 0.00% 0.00% 0.01% 0.05% 0.10% 0.50% Blank 5.98 100.0 - - - - Example 1 - - 41.63 23.79 11.45 7.28 Example 2 - - 48.12 46.59 36.73 25.44 Example 3 - - 47.18 44.55 35.93 21.71 Example 4 - - 44.07 40.73 32.10 19.49 Example 5 - - 68.32 60.92 57.38 42.84 Example 6 - - 71.62 64.87 60.29 47.86 Comparative Example 1 - - 88.55 85.69 84.07 82.19 Comparative Example 2 - - 82.74 80.31 76.89 71.17 Comparative Example 3 - - 77.27 69.47 66.67 59.37 Comparative Example 4 - - 78.56 72.93 67.29 59.75 Bisabolol - - - - - 28.11

As can be seen in Table 5 above, it was confirmed that the above example mixture containing phospholipid, Urea, Glucose, N-Acetyl Glucosamine and Mineral water Showed excellent anti-inflammatory activity as compared with the control and comparison groups. 0.1 to 1 part by weight of phosphorus-derived phospholipid, 0.01 to 0.1 part by weight of urea, 0.01 to 0.1 part by weight of glucose, 0.1 to 1 part by weight of N-acetyl glucosamine, And 90.0 to 97.0 parts by weight of mineral water, respectively, and showed excellent effects in Examples 1 to 4, and the best effects were shown in Example 1.

Test Example  3: biomimetic complex of DPPH method  Antioxidative effect measurement using

The antioxidant activity of the extract of the present invention was measured by electron donating ability to DPPH.

DPPH exhibits a strong absorption band at 517 nm due to its odd number of electrons, but if it reacts with an electron donor, it absorbs electrons or hydrogen radicals and becomes a stable molecule, and the absorption band disappears. In addition, the donated electrons are irreversibly bound to each other, and the color of purple DPPH gradually becomes weaker and the absorbance decreases.

The complexes prepared in the above Examples and Comparative Examples were used as samples, and DPPH was purchased from Sigma Co., Ltd. (Sigma CO, Ltd., USA). First, a standard DPPH solution with a concentration of 400 mu m was prepared. The samples were prepared by dissolving them in DMSO to a concentration of 10, 50, 100, 250 and 500 ppm, respectively. As a control group, ascorbic acid (500 ppm) and purified water were prepared. 100 μl of ascorbic acid and 100 μl of 0.4 mM DPPH are added to a 96-well plate and mixed. The blank of ascorbic acid and sample is mixed with 100 μl of EtOH instead of 0.4 mM DPPH. After 30 minutes of color development at 37 ° C, zero point calibration was performed at 517 nm to blank and the absorbance was measured.

DPPH activity (%) = [1- (absorbance of sample addition group) / (absorbance of no-addition group)] x 100

Sample concentration DPPH activity (%) Ascorbic acid 500ppm 95.8 Example 1 250ppm 70.9 5000ppm 94.5 Example 2 250ppm 68.1 5000ppm 82.3 Example 3 250ppm 69.9 5000ppm 85.4 Example 4 250ppm 65.8 5000ppm 83.7 Example 5 250ppm 60.1 5000ppm 51.6 Example 6 250ppm 56.9 5000ppm 49.2 Comparative Example 1 250ppm 38.1 5000ppm 42.3 Comparative Example 2 250ppm 37.9 5000ppm 41.4 Comparative Example 3 250ppm 35.8 5000ppm 43.7 Comparative Example 4 250ppm 30.1 5000ppm 40.6 Purified water 16.3

As can be seen in Table 6 above, the amount of the above example mixture containing both phospholipid, Urea, Glucose, N-Acetyl Glucosamine and Mineral water Showed excellent antioxidative activity as compared with the control and the control. In Examples 1 to 4, better effects were obtained. In Example 1, the effect was similar to that of ascorbic acid, which is a conventional antioxidant.

Test Example  4: biomimetic complex of Elastase  Active inhibitory effect

Elastase Inhibition Activity Test was conducted to test the inhibition and improvement of skin wrinkles of biomimetic complexes of Examples 1 to 6 and Comparative Examples 1 to 4. Elastase inhibitory activity was measured by adding a sample to an agar medium supplemented with elastin and placing it in an incubator under constant temperature to measure the diameter of the halo on the medium.

sample
density
(%) Halo diameter (cm)  Example Comparative Example Purified water Elastase Retinol
crystal One 2 3 4 5 6 One 2 3 4 0.01 1.4 1.5 1.5 1.5 1.5 1.6 1.6 1.5 1.5 1.6 1.6 1.6 0.8 0.05 1.0 1.2 1.3 1.2 1.4 1.5 1.6 1.5 1.4 1.5 0.10 0.8 1.0 1.1 1.1 1.2 1.3 1.6 1.4 1.4 1.3 0.50 0.7 0.9 0.8 0.9 1.1 1.1 1.5 1.2 1.3 1.1

The greater the Halo diameter, the greater the degradation of elastin. As shown in Table 7 above, when the biomimetic composite of the present invention was treated, the halo diameter decreased, It was found that the inhibitory effect was suppressed.

Test Example  5: Collagen biosynthesis effect of biomimetic complex

The collagen biosynthesis promoting effects of the biomimetic complexes of the above Examples and Comparative Examples were tested.

Fibroblasts were inoculated into IMDM medium supplemented with 10% FBS at a cell concentration of 5 × 10 ⁵ cells and cultured in a 5% CO ₂ incubator at 37 ° C. for 24 hours. The biomimetic complexes of the examples and the comparative examples were diluted in dimethyl sulfoxide to a final concentration of 100 and 500 μg / ml, and diluted solutions were added thereto, followed by culturing for 18 hours under the same conditions. After that, fibroblasts were recovered using Trizol reagent (Invitrogen, USA), and mRNA was extracted and cDNA was synthesized through a series of procedures. The gene amplification was carried out by using 10xtAq polymerase buffer, 10 mM dNTP, 10 pmol (10 pmol), and 10 pmol of dNTP using a thermal cycler (GenePro, Hangzhou bioer tech., CHINA) The reaction mixture was mixed with distilled water and adjusted to 50 μL. After 95 ° C for 5 min, 1 cycle at 95 ° C for 1 min and 51 ° C for 2 min, / 72 ° C for 1 minute 28 cycles amplification and 72 ° C for 5 minutes. The expression rate of the produced procollagen was calculated according to the following equation and normal fibroblasts without treatment were used as a control.

procollagen expression rate (%) = B / A x 100 (%)

A: The amount of procollagen expression in the control group

B: The amount of procollagen expressed in the sample treated and UV irradiated fibroblasts

COL1A1 gene expression (%) UV irradiation 0.00% 0.00% 0.10% 0.50% Blank 100 0.69 - - Example 1 - - 58.13 257.25 Example 2 - - 49.26 199.79 Example 3 - - 51.99 206.42 Example 4 - - 50.57 200.71 Example 5 - - 46.23 189.10 Example 6 - - 45.88 188.48 Comparative Example 1 - - 10.97 32.81 Comparative Example 2 - - 27.46 40.19 Comparative Example 3 - - 28.76 42.54 Comparative Example 4 - - 28.09 42.79 Retinol Crystal - - - 211.67

As can be seen in Table 8 above, the amount of the above example mixture containing all of the phospholipids, Urea, Glucose, N-Acetyl Glucosamine and Mineral water Showed excellent collagen biosynthesis promoting effect as compared with the control and comparison groups. In Examples 1 to 4, superior effects were shown, and in Example 1, the best effects were obtained.

Produce Example  1: softening longevity

The formulation of the softened longevity containing the biomimetic complex of Example 1 was prepared according to a conventional preparation method with the composition shown in Table 9 below.

ingredient Content (% by weight) Biomimetic complex Balance EDTA-2Na 0.02 1,3-butylene glycol 5.20 Oleyl alcohol 1.50 ethanol 3.20 Polysorbate 20 3.20 Benzophenone-9 2.00 Carboxyl vinyl polymer 1.00 glycerin 3.50 incense a very small amount antiseptic a very small amount system 100.0

Produce Example  2: Cream

The cream formulations containing the biomimetic complexes of Example 1 were prepared according to the usual preparation methods with the compositions shown in Table 10 below.

ingredient Content (% by weight) Biomimetic complex Balance Pro-type glycerin monostearate 2.0 Stearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Purified vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaruronate 4.0 Preservative, fragrance, pigment a very small amount system 100.0

Claims (7)

0.1 to 1 part by weight of phosphorus-derived phospholipid, 0.01 to 0.1 part by weight of urea, 0.01 to 0.1 part by weight of glucose, 0.1 to 1 part by weight of N-acetyl glucosamine, And 90.0 to 97.0 parts by weight of a mineral water (mineral water). delete The cosmetic composition according to claim 1, wherein the soy-derived phospholipid is at least one selected from the group consisting of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI). The cosmetic composition according to claim 1, wherein the biomimetic composite is contained in an amount of 1 to 99% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is for antioxidation. The cosmetic composition according to claim 1, wherein the cosmetic composition is for inflammation relief. The cosmetic composition according to claim 1, wherein the cosmetic composition is used for improving skin wrinkles and improving elasticity.