KR101775380B1 - Circular Culture Vessel of Pyogo Mushroom Sawdust - Google Patents

Abstract

Cylindrical culture vessels of large volume sawdust media consist of a cylindrical culture vessel containing a sawdust medium of high-grade type, which disperses the load during multi-stage loading to minimize deformation, and a plurality of aeration lids So that it is possible to cultivate a large amount of energy in the mycelial cells because the mycelial accumulation amount is increased because the respiration reaction during mycelial culturing is smoothly maintained in the large-sized sawdust medium. In the growth on the maintained medium, the surface area of the sawdust medium is relatively small in the one unit sterilization medium, and it is easy to manage the growth and increase the productivity.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a cylindrical culture vessel of a large-

The present invention relates to a culture container for a sawdust culture medium, and more particularly, to a culture container comprising a cylindrical culture container containing a sawdust culture medium of a high-grade type and dispersing a load during multi-stage loading to minimize deformation, The present invention relates to a cylindrical culture vessel of a large-volume sawdust culture medium.

Mushrooms are aerobic microorganisms, which can be roughly divided into the cultivation process of the mycelium growing as a nutritional growth organism and the growth process of the fruiting body, which is a reproductive growth plant. It is necessary to adequately control the carbon dioxide which is a byproduct of the respiration reaction have.

The growth conditions of the mushroom must be appropriate to the conditions of temperature, humidity, ventilation and light. In the vegetative growth period, the growth environment depends on the oxygen concentration. However, in the reproductive growth stage, depending on the stage of the maturity of the fruiting body, (Stem) growth rate and may affect the cause of malformed mushrooms.

Depending on the growth rate of mycelia, such as mushrooms are quick to Oyster mushrooms, mushrooms and shiitake mushrooms are relatively slow growth.

It is a common practice to cultivate oak logs, sawdust production, sawdust fermentation, medium mixing, sterilization, cooling, inoculation, mycelial growth, and growth stages.

When 1 unit of sawdust medium is cultivated, it is necessary to prevent air pollution such as fungi before the inoculation source is activated including maintenance of air-conditioning facilities of the incubation center and vitality maintenance of the inoculation center, and supply of oxygen necessary for respiration reaction inside the incubator Ventilation should be done to allow the generated carbon dioxide to escape in real time.

It is necessary to maintain the generated respiration rate properly in the incubation vessel for a long incubation period. Therefore, the ventilation hole for the ventilation movement and the proper discharge of the breathing water can not be increased to a large extent while preventing the contamination by the role of the lid.

Therefore, it is necessary to monitor the concentration of carbon dioxide accumulation (relative oxygen concentration can be grasped) in the incubator at the culture stage and to monitor the water content of the culture medium inside the culture vessel at the end of the culture, .

Conventional cultivation of mushroom (oak) is carried out in a mushroom cultivation box containing a sawdust medium mixed with sawdust and nutrient into a certain size bottle, and there is a problem in each homogeneity in a mycelial culture unit in a small amount. In addition, There is a problem that it is difficult to manage the growth in the growth and the biological productivity is low even if the culture is performed because the medium capacity capable of absorbing and storing the nutrient source into the cell by the biomarker such as the degradation effect of the enzyme from the nutrient source.

Conventional methods for cultivation of a high-density bag require a cultivation shelf for cultivation, and most of the hand labor is also inefficient in storing culture baskets containing 9 to 12 bags.

(12 to 16 bottles) in one basket at the time of mechanization, when the mushroom is in the inoculation position in the upper part of the culture medium, the drying condition on the upper surface of the culture medium in the culture medium form during the long culture period is severe Uniform footing is not achieved well and there is a limit of uniformity in the method of suturing.

It is difficult to collect large amounts of fruiting bodies in one cycle and the formation of fruiting bodies is formed through several cycles. Therefore, after one cycle of harvesting, such as top, fresh shoots, etc., the medium is taken out and then returned to the culture bottle. It is an efficient method to harvest fruiting bodies by proceeding with two cycles after the one-cycle harvesting and the dormant culture period by using the two-cycle method. Therefore, as the surface area of the medium becomes smaller, It is necessary to reduce the effect of the evaporation of the culture medium to maintain the life of the cultured medium.

Up to now, the sawdust culture method of a high-grade type has been described in which the weight of a single piece of sawdust medium cultivated commercially is 5 kg or less, and when viewed in detail, a rectangular medium of 2.5 kg in Japan, a 3.5- , And Taiwan's cylindrical medium of about 1.2 to 1.8 kg in the form of short-stem have been established as representative cultivation forms in each country.

The reason why the weight of 1 unit of sawdust medium is more than 5kg is that the cultivation is not smooth because only 1 or 2 vents are used for 1 unit in order to block the pollution problem. And the condition is limited by the fact that the respiration reaction can not be smoothly carried out during the period of existence, and the nutrient source can not be accumulated sufficiently in the cells.

In particular, even if the culture is performed well when the weight of the unit 1 medium is small, there is a problem that the surface area of the culture medium becomes large in comparison with the culture medium capacity of one pot, so that the moisture is excessively discharged from the culture medium, It is difficult to achieve commercial scale because productivity is low.

When the culture medium is large, the amount of aeration required for the respiration reaction is influenced by the excessive carbon dioxide concentration and the limited oxygen concentration in the culture medium during cultivation, It can not be maintained.

In order to solve the above problems, the present invention provides a method of manufacturing a sowing machine, comprising the steps of: forming a culture container containing a sawdust medium having an elevation type in a cylindrical shape to minimize deformation by dispersing a load during stacking, The present invention provides a cylindrical culture container having a large capacity sawdust culture medium, which is capable of culturing a large volume sawdust culture medium by allowing a plurality of vents to be formed at a site to facilitate a breathing reaction.

According to an aspect of the present invention, there is provided a cylindrical culture container of a large volume sawdust culture medium,

A vertical surface formed vertically downward from an edge of the upper surface and an upper cover protrusion bent at right angles in a horizontal direction at an end of the vertical surface are formed in a cylindrical shape whose cross section is opened downwardly and a plurality of vents are drilled, An upper lid to which a lid coupled to the inlet portion is coupled; And

The upper end of which is vertically erected from the bottom so as to support the inner lower surface of the upper lid to form a plurality of rod-like pedestals in the longitudinal direction, the sawdust medium of the mushroom is accommodated and the upper lid is covered Wherein the upper lid is spaced a predetermined distance inward from the vertical plane and is formed with an engaging rib which is vertically protruded downward from one side of the lower surface of the upper lid and a side wall of the lower container is formed between the vertical surface and the engaging rib And is coupled to the space.

According to the above-described construction, the present invention is advantageous in that the cleaning operation can be facilitated by using a brush that uniformly disperses the load by the cylindrical culture container and rotates the large lump particles or the contamination source when the cleaning is performed after use.

The present invention constitutes a cylindrical culture container of a high-shear saw culture medium and appropriately disperses the load during multi-stage loading, thereby minimizing breakage and deformation of the culture container.

The present invention is designed so that the mycelial culture can be smoothly performed by designing a plurality of vents so that the ventilation required for cell respiration of the mycelium is smooth during the growth period of the mycelium and that the respiration rate is properly maintained during the long culture period. The relative surface area of the medium can be significantly reduced in the growing, so that the life of the medium can be maintained for a long time and productivity of the mycelium can be maintained by maintaining the vitality of the mycelium.

1 is a view showing a configuration of a cylindrical culture container of a large-capacity sawdust culture medium for elevation cultivation according to an embodiment of the present invention.
2 is a top view of the top cover according to the embodiment of the present invention.
FIG. 3 is a top view of a lower container according to an embodiment of the present invention.
4 is a side cross-sectional view showing a cylindrical culture container of a large-capacity sawdust culture medium for elevation cultivation according to an embodiment of the present invention.
Fig. 5 is a detailed view of the side sectional view of Fig. 4. Fig.
FIG. 6 is a top view of a cylindrical culture container of a high capacity sawdust culture medium for elevation cultivation according to an embodiment of the present invention.
7 is a perspective view illustrating a durable lid of a culture medium container of a heavy sawdust culture medium according to an embodiment of the present invention.
FIG. 8 and FIG. 9 are exploded perspective views showing a durable lid of a culture container of a heavy sawdust culture medium according to an embodiment of the present invention.
10 and 11 are cross-sectional views showing a durable cap of a culture medium container of a heavy sawdust culture medium according to an embodiment of the present invention.
12 is a view showing a durable lid of a heavy sawdust culture culture container according to an embodiment of the present invention as seen from below.
FIG. 13 is a view showing a multi-stage stacking of a cylindrical culture container of a large-capacity sawdust culture medium for elevation cultivation according to an embodiment of the present invention.
FIG. 14 is a view showing a loading method in a culture chamber of a cylindrical culture container of a large capacity sawdust culture medium for elevation cultivation according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when an element is referred to as "comprising ", it means that it can include other elements as well, without excluding other elements unless specifically stated otherwise.

FIG. 1 is a view showing the construction of a cylindrical culture container of a large-capacity sawdust culture medium for elevation cultivation according to an embodiment of the present invention, FIG. 2 is a view showing a top cover viewed from above, according to an embodiment of the present invention, 4 is a side cross-sectional view showing a cylindrical culture container of a large-capacity sawdust culture medium for elevation cultivation according to an embodiment of the present invention, and Fig. 5 is a cross- 6 is a view showing a cylindrical culture container of a large capacity sawdust medium cultured in an elevation according to an embodiment of the present invention, viewed from above.

The cylindrical culture container 100 of the high capacity sawdust culture medium according to the embodiment of the present invention includes a cylindrical lower container 120 in which a sawdust medium used for culturing the mushroom is accommodated, And a cylindrical upper cover 110 opened downward to cover the lower container 120.

The upper lid 110 forms 9 to 25 vent holes 112 and preferably 12 vent holes 112 to prevent contamination and to maintain ventilation.

Here, each of the vents 112 includes a vents lower member 112a and a vents upper member 112b.

The inlet portion of the vent 112 is coupled to the durable lid 200 of the elevated sawdust culture vessel and is described in detail below in Figures 9-14.

The lower container 120 forms an inner space 121 for receiving the sawdust medium and a pedestal 123 for supporting the upper lid 110 is formed on the inner bottom surface 122 of the lower container 120 Respectively.

The lower container 120 includes a first circular reinforcing tab 124 protruding from the bottom surface 122 for preventing warping and slippage of the container and a second reinforcing tab 124 protruding along the circular rim to a size close to the diameter of the lower container 120 A circular second reinforcing tab 125 is formed.

The pedestal 123 is vertically formed from the inner bottom surface 122 so as to support the inner lower surface of the upper lid 110, and a plurality of bars are formed in the longitudinal direction.

The pedestal 123 is formed in a curved shape at its upper end and has a tapered structure for widening its width toward the lower end to stably support the pedestal 123.

The pedestal 123 is formed at the center of the first reinforcing tab 124 and at four intervals on the circular line of the first reinforcing tab 124 at regular intervals.

One pedestal 123 is formed at the center of the inner bottom surface 122, and four pedestals 123 are formed to surround the periphery of the pedestal 123 at regular intervals.

Four vent holes 112 are formed in the first reinforcing tab 124 at positions where the pedestal 123 is not provided and eight holes are formed between the first reinforcing tab 124 and the second reinforcing tab 125 at predetermined intervals .

The first reinforcing tab 124 protrudes along the circular rim at the position where the pedestal 123 is formed and the second reinforcing tab 125 protrudes along the outermost edge of the bottom surface 122 of the lower container 120.

The pedestal (123) prevents multi-stage stacking in the sterilization tank and deformation even at high temperature, and it is possible to carry out multi-stage stack culture of 12 to 18 stages in order to reduce the culture volume in the culture.

The upper cover 110 has a vertical surface 113 formed vertically downward from an edge of the upper surface and an upper cover protrusion 114 bent at right angles in a horizontal direction at an end of the vertical surface 113.

The upper lid protrusion 114 is a member for separating the upper lid 110 from the lower container 120.

The upper cover 110 is formed with a deformation preventing reinforcing rib 115 that connects between the outer surface of the air vent 112 and the outer surface of the air vent 112 adjacent thereto.

The deformation preventing reinforcing ribs 115 prevent the top cover 110 from being deformed even at the time of pasteurization and multi-stage loading.

The lower vessel 120 has a cross-section "

And a knob reinforcing rib 127 is formed along the inner side of the lower container protruding knob 126 to prevent the lower container 120 from being warped.

The upper cover 110 is spaced a predetermined distance inward from the vertical surface 113 and is formed with a coupling rib 116 vertically protruding downward from one side of the lower surface of the upper cover 110.

On one surface of the joint reinforcement strip 116, a warping rib 117 for preventing warping is formed along the rim of the circular shape inside the lower surface of the upper lid 110.

The bonding ribs 116 are intended to prevent exposure of contamination sources such as fungi before the inoculation source is proliferated.

The sidewall of the lower container 120 is fitted into the groove between the vertical surface 113 of the upper cover 110 and the coupling rib 116 of the upper cover 110 so that the gap is not opened.

The grooves between the vertical surfaces 113 and the coupling ribs 116 of the upper lid 110 serve to prevent contamination that is not exposed to contaminants such as fungi.

Thus, after the mycelium culturing, the protector does not protrude from the joining portion of the side surface of the upper container 110 and the side surface of the lower container 120.

The cylindrical culture container 100 of the present invention is configured such that the sawdust medium is charged to the uppermost end of the lower container 120 without filling the sawdust medium to the upper cover 110 in a state where the upper cover 110 is coupled to the lower container 120, .

The height of the lower container 120 is set to 130 to 140 mm and the diameter of the lower container 120 is set to 360 to 480 mm so that the wet weight of the unit culture medium unit can be 8.4 to 12.6 kg (most preferably about 11.5 kg) .

In the cylindrical culture container 100 of the present invention, the sawdust medium is contained in the lower container 120 for sterilization, and the upper cover 110 is covered to cover the upper cover 110, The condensed water is shaken out of the upper lid 110, and after the inoculation, it is closely adhered again.

Since the inner space of the upper cover 110 is formed in the mycelial culture, the sawdust medium is filled to a height below the height of the lower container 120 so as to serve as a cushion of moisture discharged to the outside.

Thus, the mycelium in the culture rises on the base wall to completely prevent the hyphae from being inserted into the sponge filter 229 mounted inside the durable lid 200, which opens and closes the vent 112, so that hyphae of the sponge filter 229 Do not generate at all.

FIG. 7 is a perspective view showing a durable lid of a culture medium container of a heavy sawdust culture medium according to an embodiment of the present invention, and FIGS. 8 and 9 are exploded perspective views showing a durable lid of a culture medium container for a heavy sawdust culture medium according to an embodiment of the present invention, 10 and 11 are cross-sectional views illustrating a durable lid of a culture medium container of a heavy sawdust culture medium according to an embodiment of the present invention, and FIG. 12 is a view showing a durable lid of the culture medium container of a high- Fig.

The durable lid 200 of the culture dish 100 of the heavy sawdust culture culture medium 100 according to the embodiment of the present invention performs the ventilation required for the mycelial breathing reaction with the lid coupled to the ventilation hole 112 of the culture medium container 100 of the heavy sawdust culture medium.

The durable lid 200 includes an outer lid 210 and an inner lid 220 inserted and coupled to the inside of the outer lid 210.

The outer lid 210 is formed of a cylindrical outer body 211 having a cylindrical shape and opened downward in cross section, an inner space 212 having a predetermined depth from the inner side of the outer body 211, And a seating portion 213 protruding outwardly from the lower end along the rim of the outer body 211.

A protruding support 214 for supporting a load is formed on one side of the inner surface of the outer body 211 so as to protrude in a circular arc at a predetermined height and is provided with a hollow space between the protruding support 214 and the adjacent protruding support 214. An air inlet 214a through which air flows and flows out is formed.

At one side of the inner surface of the outer body 211, a fixing tab 215 protrudes horizontally at a lower position than the protrusion support 214.

The fixing tabs 215 are formed on the inner side surface of the outer body 211 at a plurality of different heights with respect to the open end.

The fixing tab 215 serves as a latching jaw to prevent the outer lid 210 from being easily detached when the outer lid 210 is engaged with the inner lid 220.

The inner lid 220 is formed of a plastic material and has an upper body 221 formed with a cylindrical rim of a predetermined height and having a through hole penetrated vertically through the inside thereof, A lower cylindrical inner body 222 and an inner body 222 in a through hole of the upper body 221 and an upper body 221 and an inner body 222 by a connecting rod 224.

The upper surface 223 of the inner body 222 is located at a stepped edge from the upper edge surface of the upper body 221 to a predetermined depth.

The inner body 222 and the upper body 221 are connected to each other by a connecting rod 224 connecting the upper side edge of the inner body 222 and one side of the inner side of the upper body 221 along the inner side edge of the upper body 221 And formed radially.

The ventilation space 225 spaced between the connection block 224 and the neighboring connection block 224 serves to introduce air into the lower container 120 through the external air. do.

The upper surface 223 of the inner body 222 is formed with a through hole 226 through which air flows in and out at the central portion and a ring shaped protrusion tube 227 protruding upward from the rim on which the through hole 226 is formed, Is formed. The protruding pipe 227 protrudes higher than the upper surface 223 of the inner body 222.

The upper surface 223 of the inner body 222 has an arc-shaped filter fixing member 224 spaced apart from the annular projection 227 by a predetermined distance and spaced circumferentially at regular intervals, 228 are formed.

A sponge filter 229 in the form of a donut having a through hole 226 formed at its center is inserted and mounted in a space between the protruding pipe 227 and the filter fixing member 228.

The upper surface 223 of the inner body 222 radially forms a projecting reinforcing tab 230 in the longitudinal direction for holding the load on the surface on which the sponge filter 229 is mounted.

The protruding reinforcing tab 230 is formed in the longitudinal direction extending from the inner surface of the inner body 222 to the rear surface of the filter fixing member 228.

Some protruding reinforcing tabs 230 are engaged with the rear surface of the filter fixing member 228 and some of the protruding reinforcing tabs 230 are formed from one side of the inner surface of the inner body 222 to the filter fixing member 228, And is formed so as to protrude to a space between the fixing members 228.

The lower surface 232 of the inner body 222 is formed to have a larger diameter than that of the first and second invasion preventing protrusions 231 and 231 protruding at a predetermined height downward from the rim formed with the through- And a circular second infiltration restricting tuck 231 protruding downward at a predetermined height in a form spaced apart from the first infiltration preventing tuck 231 by a predetermined distance so as to surround the first invasion preventing tuck 231.

In the large-capacity culture method of the present invention, since the culture medium is filled only in the lower container 120, there is a considerable empty space on the side of the upper lid 110, so that the hyphae in the culture are separated from the sponge filter 229 inserted between the upper durable lid 200, The mycelium is not invaded.

Therefore, in this development, since the sponge filter 229 in the lid 200 coupled to the cylindrical culture container 100 for the heavy sawdust medium can be used semi-permanently once inserted, there is almost no need to replace the sponge filter 229, The coupling may be designed so that it is not easily separated.

FIG. 13 is a view showing a multi-stage stacking of a cylindrical culture container of a high capacity sawdust culture medium for elevation cultivation according to an embodiment of the present invention, and FIG. 14 is a cross- Of the present invention.

When the cylindrical culture container 100 is stacked on the pallet 300 in multiple stages, deterioration phenomenon such as reduction of the enzyme action due to hygroscopic heat due to hygroscopicity of hyphae occurs at the central portion of the pallet 300 of the rectangular container However, when the culture container of the present invention is formed into a cylindrical shape as in the culture container of the present invention, there is an advantage that the risk is low in the middle culture stage where the heat transfer is good and the respiration action is vigorous.

The cylindrical culture container 100 has an effect of uniformly dispersing the load, and the cleaning operation can be facilitated by using a brush that rotates against large lumps of particles or contamination sources when cleaning after use.

The heavy-duty sawdust culture culture vessel 100 of the present invention is formed in a cylindrical shape, and the durable lid 200 of the present invention is used to appropriately disperse loads during multi-stage loading to minimize damage and deformation of the culture vessel 100.

The lower container 120 separating the outer lid 210 restricts the number of the pedestal 123 to 4 to 6, filling the sawdust medium, and then forming 8 to 16 perforations in the upper portion of the sawdust medium, And can form suitable voids in sawdust media.

As shown in FIG. 14, when the cylindrical culture container 100 is stacked on the pallet 300 in multiple stages, nine cells are arranged at the bottom and nine at the bottom.

When the cells are stacked in such a multi-stage manner, a temperature difference occurs between the upper and lower cylindrical culture vessels 100, which causes disadvantages in the difference in culture temperature.

In order to compensate for this disadvantage, the upper and lower culture vessels 100 and the nine lower culture vessels 100 are changed in accordance with the culture, the medium culture period, and the culture period, .

The embodiments of the present invention described above are not implemented only by the apparatus and / or method, but may be implemented through a program for realizing functions corresponding to the configuration of the embodiment of the present invention, a recording medium on which the program is recorded And such an embodiment can be easily implemented by those skilled in the art from the description of the embodiments described above.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, It belongs to the scope of right.

100: cylindrical culture container 110: upper cover
112: ventilation hole 112a: ventilation hole lower member
112b: Ventilation hole upper member 113: Vertical plane
114: upper cover protrusion 115: deformation preventing rib
116: jointing barrel 117: reinforcing rib for anti-warping
120: lower vessel 121: inner space part
122: bottom surface 123: pedestal
124: first reinforcing tab 125: second reinforcing tab
126: protruding handle 127: handle reinforcing rib
200: Durable lid 210: Outer lid
211: outer body 212: inner space part
213: seat part 214:
214a: air inlet 215: securing tab
220: inner lid 221: upper body
222: inner body 223: upper surface
224: connecting rod 225: ventilation space
226: through hole 227: projecting tube
228: Filter fixing member 229: Sponge filter
230: protruding reinforcing tab 231:
232: Lower surface

Claims (6)

A vertical surface formed vertically downward from an edge of the upper surface and an upper cover protrusion bent at right angles in a horizontal direction at an end of the vertical surface are formed in a cylindrical shape whose cross section is opened downwardly and a plurality of vents are drilled, An upper cover to which a lid coupled to an inlet portion is coupled and in which a deformation preventing reinforcing rib is formed to connect between an outer surface of the vent hole and an outer surface of a neighboring vent hole; And
The cross-section "

And the upper end of the lower container protruding knob 126 is formed into a cylindrical shape having an upper end open to the upper side and a lower end of the lower container protruding knob 126, And a lower container which is vertically erected from the bottom surface so as to support the lower surface to form a plurality of rod-like pedestals in the longitudinal direction and in which the sawdust medium of the mushroom is accommodated and the upper cover is covered,
The lower container has a circular first reinforcing tab protruding from the bottom surface to prevent warping and slippage of the container and a circular second reinforcing tab projecting along the circular rim to a size close to the diameter of the lower container, The pedestal has a pedestal at the center of the first reinforcing tab, four pedestals formed at regular intervals on the circular line of the first reinforcing tab, and the vent is positioned inside the first reinforcing tab at a position where the pedestal is not present Eight are formed at regular intervals between the first reinforcing tab and the second reinforcing tab,
The upper lid is spaced a predetermined distance inward from the vertical surface, and an engaging rib that is vertically protruded downward from one side of the lower surface of the upper lid is formed. On one surface of the engaging rib, Wherein the reinforcing rib is formed along the rim of the circular shape and the sidewall of the lower container is fitted in the space between the vertical surface and the mating reinforcing bar. delete delete The method according to claim 1,
The vent holes of the upper lid are formed in 9 to 25 cavities. The pedestals are formed at the center of the inner bottom surface and are spaced apart from each other at regular intervals to surround the periphery of the pedestal. A cylindrical culture container of a large-sized sawdust culture medium which is formed in a curved shape in a circular shape and has a tapered structure for widening its width toward the lower end and stably supporting the same. The method according to claim 1,
Wherein the height of the lower container is 13 to 14 cm and the diameter of the lower container is 36 to 48 cm so that the capacity of the sawdust medium contained in the lower container is 8.4 to 12.6 kg. delete

Images