KR101729544B1 - Cosmetic composition for improving skin transparency and skin whitening containing Sigesbeckia Orientalis Extract and Rabdosia Rubescens Extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for enhancing skin transparency and skin whitening, and more particularly, to a composition for enhancing skin transparency and skin whitening, and more particularly, to a cosmetic composition for enhancing skin transparency and skin whitening by incorporating a scarlet extract (Sigesbeckia Orientalis Extract) and a Rabdosia Rubescens Extract to increase glutathione expression and inhibit melanin production The present invention relates to a cosmetic composition which improves the skin brightness and improves the uniformity of the amount and arrangement of collagen, thereby improving the skin clear and transparent.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving skin transparency and skin whitening comprising an extract of Alaska pollack,

The present invention relates to a cosmetic composition for enhancing skin transparency and skin whitening, and more particularly, to a composition for enhancing skin transparency and skin whitening, and more particularly, to a cosmetic composition for enhancing skin transparency and skin whitening by incorporating a scarlet extract (Sigesbeckia Orientalis Extract) and a Rabdosia Rubescens Extract to increase glutathione expression and inhibit melanin production The present invention relates to a cosmetic composition which improves the skin brightness and improves the uniformity of the amount and arrangement of collagen, thereby improving the skin clear and transparent.

When skin is exposed to ultraviolet rays, melanin, which determines the skin color of a person, is synthesized and released, causing hyperpigmentation such as stain, freckles, and dots, resulting in poor cosmetic results. The synthesis of melanin begins with tyrosine as a substrate, tyrosinase produces dopaquinone, autooxidation and enzymatic reaction from dopaquinone, and melanin synthesis do. Therefore, in order to prevent the skin color from becoming black, a method of reducing melanin synthesis itself by inhibiting some reactions during melanin production has been studied. Representative whitening agents known to inhibit the synthesis process include kojic acid, arbutin, vitamin A, and vitamin C.

However, they have not been able to achieve a satisfactory whitening effect on consumers in terms of functionality. The recent trend of whitening cosmetics is a tendency to expand to a wider meaning such as clear skin color and transparency from the point of merely aiming at white skin which removed an abnormally expressed melanin pigment in the past. However, the existing whitening raw materials having a melanin synthesis inhibiting function could not sufficiently meet the needs of such customers.

In recent years, research has been actively conducted on factors affecting skin color and skin transparency in addition to melanin. Recently, collagen and hemoglobin have also been recognized as chromophores in skin. In addition to simple skin pigment, various factors controlling light scattering and refraction in real skin have been found to determine complexity of skin to determine skin transparency. Melanin, uniformity, moisture content, hemoglobin, etc. are considered to be major influential factors. Melanin not only makes skin color black, but also makes skin look slimy by hindering the reflection of light on skin. When blood circulation in skin microcirculation is stagnated due to aging, the redness increases and the uniformity is decreased. do. Particularly, the roughness of the skin surface, which is determined by the fine bending of the skin and the keratin, has a direct influence on the reflection of light, and uniform arrangement from the inside of the skin has been studied to determine the transparency to the skin.

Finally, moisture inside the skin refracts light and is known to increase transparency. In the same research context, studies on the difference between baby skin with high transparency and adult skin have been actively carried out. Baby skin has less stain and uniformity than adult skin, The results have been published. If this effect can be realized with cosmetics, it is expected that it will satisfy customer's ultimate whitening satisfaction.

Accordingly, the inventors of the present invention have conducted studies on various plant herb extracts in order to find a raw material recognized for enhancing human safety and transparency of skin. As a result, The present inventors have found that the tight arrangement can be restored and the passage through which the light exits from the inside of the skin can be rearranged to improve the transparency.

Accordingly, it is an object of the present invention to provide a cosmetic composition which has an increased antioxidant enzyme glutathione, inhibits melanin production, and improves the amount of collagen and the uniformity of the arrangement, thereby improving skin transparency.

In order to accomplish the above object, the present invention provides a cosmetic composition for promoting skin transparency and skin whitening comprising a whiteness extract and an extract of Echinochlofenac as an active ingredient.

The pleiotropic extract of the present invention and Dongryungcho extract promotes the expression of glutathione, an antioxidant enzyme, inhibits melanin production, thereby improving the skin brightness and restoring the uniform and compact arrangement of collagen, Skin transparency and whitening.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the skin transparency enhancing effect of the Nos.
FIG. 2 is a graph showing the skin thickness and density enhancement effect by the Nosquito extract and the Nos.
Fig. 3 is a cross-sectional photograph of skin showing the effect of increasing the skin compactness by the Nosquito extract of the present invention.

The present invention provides a cosmetic composition for enhancing skin transparency and skin whitening comprising, as an active ingredient, an excipient extract (Sigesbeckia Orientalis Extract) and a Rabdosia Rubescens Extract.

Hereinafter, the present invention will be described in more detail.

Sigesbeckia Orientalis, used as an active ingredient in the present invention, is an annual herbaceous herbaceous plant of the dicotyledonous plant L. longifolia, and is known to be effective for arthritis and blood pressure lowering. It is also used as a herbal medicine and used for the treatment of arthritis, hypertension, jaundice and boils.

Rabdosia Rubescens, which is used as an active ingredient in the present invention, is a perennial herb that has been used as a traditional medicinal herb in China and has been used as a traditional medicinal material in China. According to the Cancer Research Institute of Hannam University of Medicine in China, ) Inhibit the synthesis of cancer cell DNA and RNA.

The scarlet or perennial herb extract of the present invention can be obtained by a conventional extraction method in the art, and the method is not particularly limited. For example, 1 kg of scavenge or tungsten syrup is added to 5 liters of water, an organic solvent (ethanol, methanol, butanol, ether, ethyl acetate, chloroform or methylene chloride) or a mixed solvent thereof, refluxed three times, Day. Thereafter, the residue and the filtrate are separated by filtration through a filter cloth and centrifugation, the separated filtrate is concentrated under reduced pressure, and the resulting extract is suspended in 1 liter of water, followed by extraction five times with 1 liter of methylene chloride. The methylene chloride layer thus obtained is concentrated under reduced pressure to obtain 50 g of a lycopersicon esculent extract.

The cosmetic composition for enhancing skin transparency and skin whitening according to the present invention contains an extract of Nomad extract and a extract of Nomad extract in an amount of 0.001 to 10.0% by weight based on the total weight of the composition.

If the content of each of the exholiated extract and the perennial herb extract is less than 0.001% by weight based on the total weight of the composition, it is difficult to expect skin whitening and skin transparency enhancement effect. If the content exceeds 10.0% by weight, The efficiency of the raw material is deteriorated, and the feeling of use of the formulation is greatly deteriorated, thereby decreasing the value as a cosmetic product.

The cosmetic composition of the present invention contains the whiplash extract and Dongryungcho extract at a weight ratio of 1: 1 to 5: 1. When the mixing range is out of the above range, the synergistic effect of the active ingredients is insignificant and it is difficult to exhibit skin transparency and skin whitening effect.

Lumi White, which is a mixture of the extract of Eucalyptus according to the present invention and the extract of Eucalyptus extract, is a combination based on scientific basic data on the skin, and when the Lumi White is used for skin, it realizes the long-term efficacy in terms of skin transparency enhancement and skin whitening .

The cosmetic composition of the present invention is not particularly limited in its formulation and may be, for example, a cosmetic composition having a formulation of a softening longevity, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel or a skin adhesive type cosmetic.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples, but the scope of the present invention is not limited to the following examples and test examples.

[Preparation Example 1] Preparation of a squalane extract

1 kg of scallop was placed in 5 L of ethanol, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation. The separated filtrate was concentrated under reduced pressure, and the obtained extract was suspended in 1 liter of water, followed by extraction 5 times with 1 liter of methylene chloride. The methylene chloride layer thus obtained was concentrated under reduced pressure to obtain 50 g of the eluate extract.

[Preparation Example 2] Preparation of Dongryungcho extract

One kilogram of Dongryungcho was added to 5 liters of ethanol, refluxed three times, and then immersed at 15 캜 for one day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation. The separated filtrate was concentrated under reduced pressure, and the obtained extract was suspended in 1 liter of water, followed by extraction 5 times with 1 liter of methylene chloride. The methylene chloride layer thus obtained was concentrated under reduced pressure to obtain 50 g of Dongryungcho extract.

[Examples and Comparative Examples 1 to 3]

Examples of O / W lotion formulations and Comparative Examples 1 to 3 having the compositions shown in the following Table 1 were prepared. The viscosity of the lotion formulation was measured after putting it in a thermostat at 30 ° C. after the preparation. The viscosity of the lotion formulation was 5000 to 10000 cp and the pH (based on 10% aqueous solution) was 6.5 to 7.5.

Component name (unit:% by weight) Example Comparative Example One 2 3 1. Purified water To 100 To 100 To 100 To 100 2. Glycerin 3.0 3.0 3.0 3.0 3. Butylene glycol 5.0 5.0 5.0 5.0 4. Carbomer 0.1 0.1 0.1 0.1 5. Excipient extract (Preparation Example 1) One - One - 6. Dongryungcho extract (Preparation Example 2) 0.5 - - 0.5 7. Zanthan gum 0.1 0.1 0.1 0.1 8. Triethanolamine 0.1 0.1 0.1 0.1 9. Hydrogenated polydecenes 2.0 2.0 2.0 2.0 10. Cetearyl glucoside 1.2 1.2 1.2 1.2 11. Sorbitan stearate 0.4 0.4 0.4 0.4 12. Cetearyl alcohol 0.5 0.5 0.5 0.5 13. Caprylic capric triglyceride 5.0 5.0 5.0 5.0 14. Preservatives Suitable amount Suitable amount Suitable amount Suitable amount 15. Incense Suitable amount Suitable amount Suitable amount Suitable amount

<Manufacturing Method>

1) The oil phase components 9 to 14 were heated to 70 to 75 캜 and mixed uniformly.

2) The above water phase components 1 to 3 and 5 to 8 were heated to 70 to 75 캜 and uniformly dissolved and mixed.

3) The mixture of 1) was added to the mixture of 2) at a temperature of 70 to 75 ° C with stirring to prepare an emulsion.

4) The components 15 and 4 were continuously added to the emulsion of the above 3) and then cooled to 28 to 30 캜.

[Test Example 1] In vitro inhibition of melanin formation

Human melanoma cells, HM3KO cells (Y. Funasaka, Department of dermatology, Kobe University School of Medicine, 5-1 Kusunoki-cho 7-chrome, Chuo-ku, Kobe 650, Japan) Essential Medium (MEM) and cultured at 37 ° C and 5% CO ₂ . The cells thus cultured were dispensed into a 75-well flask so that the cell number was 3 × 10 ⁵ per well, and the cells were waited for overnight attachment to the wall. After confirming that the cells were well adhered, the medium was changed to a new medium containing 10 ppm of each test substance. As the test material, the above-mentioned Examples and Comparative Examples 1 to 3 were used, and a medium containing only DMSO was used as a control.

The cells were cultured until the cells were filled in the flask by changing to a new medium containing the sample once every two days. Subsequently, the culture solution was removed, washed with PBS, and dissolved in 1 N sodium hydroxide. The absorbance at 500 nm was measured, and the inhibitory rate of melanin production of the samples to the control group was calculated according to the following formula (1) .

Test substance Melanin production inhibition rate (%) Example 22.1 Comparative Example 1 1.5 Comparative Example 2 10.5 Comparative Example 3 6.3

The results of the above Table 2 show that melanin production was suppressed in Comparative Examples 2 and 3, which contained the active ingredients of the present invention, Was able to most effectively inhibit the production of melanin.

Therefore, it was confirmed that the cosmetic composition containing the wholesaled extract according to the present invention and the Dongreung herbal extract as an effective ingredient effectively inhibits melanin production due to the synergy effect between the active ingredients, thereby exerting a skin whitening effect.

[Test Example 2] Whitening effect test on human skin

Ten healthy men were asked to attach an opaque tape with six holes (1.5 cm in diameter) to the subject's neck and then subject the subject to UVB of 1.5 to 2 times the minimum erythema dose (Minimal Erythema Dose) The darkening of the skin was induced by irradiation, the test materials were applied, and after two months, the darkness and darkness of the skin were measured using a chroma meter. As the test materials, the above-mentioned Examples and Comparative Examples 1 to 3 were used, and each test substance was applied twice a day (morning and evening) daily.

The determination of the whitening effect was determined by determining the "L" value representing the shade of the skin (the non-burned Korean skin color generally has a value of 50 to 70). When the test substance is applied, the L value gradually increases when the test substance is effective. The comparison between the test substances is expressed by a ΔL value (final L value - L value before application of the test substance). The larger the value of? L, the greater the whitening effect. The mean values of? L for 10 subjects according to the experimental results are shown in Table 3 below.

Test substance Whitening effect (? L) Example 3.21 Comparative Example 1 0.27 Comparative Example 2 2.1 Comparative Example 3 1.42

As can be seen from the above Table 3, Comparative Examples 2 and 3 each containing the Nosquito extract of the present invention exhibited higher whitening effect than Comparative Example 1 which did not contain all of the Nosquito extract and Dongryungcho extract, Showed the best skin whitening effect.

Accordingly, it was found that the cosmetic composition containing the wholesaled extract according to the present invention and the extract of Tongrenchu extract as an active ingredient has a superior skin whitening effect due to the synergy effect between the active ingredients.

[Test Example 3] Skin transparency enhancement confirmation test

Twenty women in their 30s and 40s were examined for skin clarity before the start of the study. Twenty women (female with skin irritation, spots, and erythema) were examined for skin clarity. ± 2%) after 15 minutes of adaptation. The above-mentioned example, which contains the extract of Eisai and the extract of Dongryungcho as an active ingredient, was used twice in the morning and evening, and re-measurement of skin clarity was carried out at 2 weeks, 4 weeks and 8 weeks.

Specifically, the method of measuring the transparency of a skin is to measure the amount of light reflected from a halogen lamp at an angle of 20 to 70 ° at an interval of 10 ° after the light from the light source is incident on the skin surface at an angle of 45 °. Both incident light and reflected light are controlled through a polarizing filter, and a combination of two polarizing filters is used to measure skin surface reflection and diffuse reflection respectively. First, the value measured when the polarization plane of the light source / light receiving unit is used in the same direction is a, and the value measured when the polarization plane in the perpendicular direction is used is b. The light a measured when using the same direction of polarization plane includes half of the surface reflected light which is reflected from the surface of the skin and the direction of the polarization does not change and the inside reflection light whose polarization direction disappears while passing through the inside of the skin. And the value b measured when using the polarization plane in the perpendicular direction has no surface reflected light and only 1/2 of the diffused reflected light exists. Therefore, the amount of surface reflection / diffuse reflected light is calculated by the following equation (2), where the internal reflected light is used as an index of transparency and the change in the sum of values between 30 and 70 is compared. The results are shown in Fig.

As can be seen from FIG. 1, the cosmetic composition containing the excipient extract according to the present invention and Dongryungcho extract as an active ingredient increases the amount of the reflective material inside the skin that can reflect light as the application period becomes longer, To improve the skin's brightness and transparency.

[Test Example 4] Skin thickness and dermal density measurement test

To determine the changes in skin thickness and density of dermis, skin measurements were performed with an ultrasonic imaging device (Dermascan C). This experiment was conducted on the same experimental group as in Test Example 3 above. Dermascan C is an ultrasound measuring device that displays epidermis and dermis images in real time and can measure the thickness and density of epidermis and dermis.

The specific test method was to directly contact the surface of the skin to be measured using a Dermascan C probe, in which an appropriate amount of gel was loaded, and there was no air bubble between the device and the skin. Two - dimensional cross - sectional images were analyzed through the B scan mode of the measured photographs. The more bright the image, the more highly echogenic and the higher the amplitude, the higher the density. FIG. 2 shows skin thickness and skin density calculated by image analysis, and FIG. 3 shows skin cross-sectional photographs through Dermascan C. FIG.

As can be seen from FIGS. 2 and 3, the cosmetic composition containing the excipient extract according to the present invention and Dongryungcho extract as an active ingredient increased the skin thickness by about 0.11 mm (about 6%) after 8 weeks of application compared with before skin application The skin density was improved by about 0.34 (about 3%), and the skin texture was more uniform and dense in the skin cross - section photograph.

Therefore, it was found that the cosmetic composition containing the wholesaled extract according to the present invention and the Tongrenchu extract as an active ingredient improves the uniformity of the amount and arrangement of collagen, thereby improving the skin thickness and skin compactness, thereby improving skin transparency.

[Test Example 5] Effect of promoting glutathione (GSH) expression

The keratinocytes (keratinocyte) human 10㎝ diameter of the round cell type into a 5 × 10 ⁵ dog on a culture plate was attached for 24 hours. After 18 hours, the culture medium was removed, and 2 ml of phosphate buffered saline (PBS) was added to each well. This melanocyte-forming cells were irradiated with ultraviolet rays of 30 mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UVB 15W, Sankyo Dennki, Japan), and PBS was taken out. Was added. The sample was treated again and the amount of GSH changed by ultraviolet stimulation was determined at certain time intervals. The GSH quantitation was carried out in the presence of 5-thio-2-nitrobenzoate, which is produced by reducing GSH with 5,5-dithiobis-2-nitrobenzoic acid. nitrobenzoate) (Akerboom & Sies, 1981, Methods Enzymol. Vol 77, pp 373-382). The results are shown in Table 4 below.

Medium condition GSH (mmol / mg protein) 0 hours 1 hour after UV irradiation 3 hours after UV irradiation Control group 105.2 90.3 87 UV B 105.2 74 45 The extract (50ppm) 105.2 163 149 Dongryungcho extract (50ppm) 105.2 143 125 (50ppm) +
Dongryungcho extract (50ppm) 105.2 195 156

As can be seen from Table 4, the amount of GSH was increased compared to the untreated control group when each of the active ingredients of the present invention, When the extracts were treated together, the expression level of antioxidant enzyme, GSH, was significantly increased.

Claims (10)

A cosmetic composition for increasing skin thickness and improving the denseness comprising Siglebeia Orientalis Extract and Rabdosia Rubescens Extract as an active ingredient. The cosmetic composition according to any one of claims 1 to 3, wherein the excipient extract is in an amount of 0.001 to 10.0% by weight based on the total weight of the composition. 2. The cosmetic composition according to claim 1, wherein the excipient extract and the rhizome extract are contained in a weight ratio of 1: 1 to 5: 1. A cosmetic comprising the cosmetic composition of any one of claims 1 to 3. 5. The cosmetic product according to claim 4, having any one of the formulations selected from the group consisting of a softener, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel, or a skin stick type cosmetic. delete delete delete delete delete

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