KR100429590B1 - Anti-wrinkle cosmetics comprising Ecklonia cava extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a natural extract that promotes collagen synthesis as an active ingredient. Specifically, the present invention relates to a cosmetic composition for improving wrinkles containing Ecklonia cava extract and a method for manufacturing the same, a method for alleviating wrinkles of the skin using the cosmetic composition, a composition for promoting collagen synthesis and promoting collagen synthesis using the same Provide a method. The cosmetic composition promotes cellular activity and collagen synthesis of fibroblasts, thereby relieving wrinkles and imparting elasticity to the skin.

Description

Anti-wrinkle cosmetics comprising Ecklonia cava extracts

The present invention relates to a cosmetic material and cosmetic composition for improving wrinkles and enhancing skin elasticity, and more particularly, to a cosmetic material and cosmetic composition comprising the Ecklonia cava extract for promoting collagen synthesis as an active ingredient.

Skin aging can be divided into two categories, internal aging and external aging, depending on the cause. Intrinsic aging is an aging process that occurs naturally as the body ages and the physiological function of the body decreases. Extrinsic aging refers to aging caused by external factors such as ultraviolet (UV), dry air, reactive oxygen species and stress. As the skin ages due to these internal and external factors, the most common symptom is wrinkle formation on the skin surface.

There are many known pathways for generating skin wrinkles. For example, when the skin is exposed to excessive ultraviolet light, collagen breakdown, which is one of the main components of the skin, is promoted, causing the skin to lose elasticity and wrinkles. When the skin is dried due to temperature change, humidity decrease or wind, the skin function as a protective barrier against the outside is degraded and wrinkles are generated. Furthermore, when the skin is exposed to noxious reactive oxygen species or free radicals, lipid peroxide may be produced by oxidative action, and skin constituent proteins such as collagen may be modified to produce wrinkles.

Cosmetics and medicines to improve skin wrinkles have been continuously developed to date. Currently, cosmetics such as sunscreens, moisturizers and antioxidants are widely used to prevent wrinkles caused by external factors. However, the effect of these products on substantially improving wrinkles is still small.

Collagen is one of the major proteins that make up the skin with elastin. The amount of collagen decreases significantly with age and UV exposure, which decreases collagen protein, causing the skin to lose elasticity and wrinkles. The literature reports that the amount of collagen type I decreases as photo-aging of human skin progresses (Shuster, S. et al., Br. J. Dermatol., 93, 639-643 (1985)). . The relationship between the amount of collagen and wrinkles of the skin exposed to the sun's rays has also been reported (Chen S. and Kiss I., J. Inv. Derm., 98, 248-254 (1992)), and retinoic acid is collagen. There is also research showing that Type I is restored (Christopher E. et al., The New England J. of Medicine, 329, 530-535 (1993)). Many of these evidences support that wrinkles are suppressed as collagen synthesis increases.

Therefore, substances that promote collagen synthesis have been developed as skin wrinkle improving substances. Typical examples are Retinoic acid, Retinol (vitamin A) and vitamin C. In addition to the retinoic acid, retinol (vitamin A), betulinic acid (betulinic acid; US Patent Publication US 5,529,769), Foskolin (Hakeda, Hakeda, Y. et al., J. Biochem., 101, 1163-1469 (1987)) , Bertholletia extract (US Pat. No. 6,004,568), Landing (Radix Phytolacca esculenta ) or Semen Cuscutae extract (Republic of Korea Patent Publication No. 2000-13588), Agastache rugosa , Kwak ( Lagenaria siceraria var) clavata Ser.) and Aquilaria agallocha Roxb. extracts (Korean Patent Publication No. 2000-18467) and the like are those described as antiwrinkle cosmetics that promote collagen synthesis.

Retinoic acid inhibits lipid peroxidation by UV-A and ornithine decarboxylase response by UV-B and improves skin photodamage (Francz Pl et al. Biol. Chem. 379, 10, 1263-9 (1998)). However, skin irritation is so strong that it is currently used only clinically. FDAs in the U.S. and Korea prohibit their use as cosmetic ingredients.

Retinol (Vintine A) improves the wrinkles of skin compared to retinoic acid, but it is the most widely used anti-wrinkle agent due to its high safety. However, retinol also causes problems such as skin irritation when used in high concentrations, especially when exposed to ultraviolet light is known to cause strong skin problems. Furthermore, retinol has a disadvantage in that its stability is very low and is easily oxidized by oxygen, light and heat in the air (Korean Patent Publication No. 10-0271315).

The literature reports that high concentrations of vitamin C have an effect of improving skin wrinkles (WO 1998/50012). However, vitamin C is also easily deteriorated by air, moisture, temperature, etc., and the bioactive activity is reduced by the alteration, so the degree of wrinkle improvement is lowered, so that the stability of vitamin C remains to be overcome in order to use it as a cosmetic material.

Recently, much attention has been focused on functional cosmetics having antioxidant, wrinkle relief, whitening, and the like functions obtained from natural extracts. For example, US Pat. No. 5,972,341 describes the antiwrinkle effect of a plant of the genus Commiphora, in particular Commiphora mukul ; Japanese Patent Application Laid-Open No. 9-672662 A2 describes seaweed extracts having a hyaluroronidase inhibitory effect; Japanese Patent Application Laid-open No. Hei 10-85905 A2 describes the skin texture improving effect of Fucoidan extracted from Wakame; Japanese Patent Laid-Open No. 2-245087 A2 describes the antioxidant effect of the extract of Sargassum; Japanese Patent Application Laid-open No. 3-294384 A2 describes an antioxidant composition extracted from the genus Hizikia; Japanese Patent Laid-Open No. 7-10769 A2 describes a extract of Phaeophyceae river with an astringent effect. Natural extract cosmetics are harmless to the human body and easy to mass produce, and thus have great advantages as cosmetics.

Therefore, the present inventors have completed the present invention by finding that the extract of Ecklonia cava , a kind of natural seaweed, has an excellent effect of promoting collagen synthesis and alleviating wrinkles, while researching wrinkle improvement materials derived from natural products.

The present invention provides an anti-wrinkle cosmetic composition containing Ecklonia cava extract and a method for producing the same.

The present invention provides a method of emulsifying the skin and relieving wrinkles by using the cosmetic composition containing the Ecklonia cava extract.

The present invention provides a composition for promoting the synthesis of collagen comprising Ecklonia cava extract and a method for promoting the synthesis of collagen using the same.

The composition for improving wrinkles of the present invention is characterized by containing an extract of Ecklonia cava as an active ingredient. Eckloniatic compositions can be used with carriers that can be used in the manufacture of cosmetics. In the definition of the present invention, "wrinkle relief" means reducing the depth of wrinkles or reducing the number or area of wrinkles, so that "wrinkle relief", "wrinkle improvement" and "improve skin elasticity" are mutually Used synonymously.

Ecklonia cava is a perennial seaweed belonging to the kelp family of brown seaweed. Distributed on the south east coast, south coast and Jeju island, it is the most mature in spring. The stem is a thick cylinder, and the upper part is the flat leaf part. In the spring, between the stem and the fronds, a new frond grows for 3-4 years. The color is brown but when it is dry it becomes black and the quality is leather. Dried Ecklonia has been used as a raw material for alginic acid or as a fertilizer for bulb crops, but it has not been developed as a high value-added food, medicine, or cosmetic (Kang, JW Bull. Pusan Fish. Coll., 7, 1-125 , 12; (1966)).

The present invention provides a solvent extract of Ecklonia cava which can be usefully used as a cosmetic material, Ecklonia cava extract suitable for the purpose of the present invention can be prepared according to conventional extraction methods of drying, grinding, extraction, filtration and concentration.

Ecklonia cava, which is the material for extraction, can be used by drying it or as it is. It is preferable to use it by drying, At this time, natural drying or hot air drying can be used as a drying method. In the embodiment of the present invention was used to wash the Ecklonia cava in purified water and naturally dried in the shade. Purified water is water sterilized by illuminating ion-exchanged water with an ultraviolet lamp to remove bacteria and metal ions. The dried Ecklonia cava may be used by pulverizing with a grinder to facilitate solvent extraction and may be used without distinction of size, but preferably, pulverized to a size of 2 to 3 cm.

Finely chopped or dried dried Ecklonia cava extract is used as a solvent. Ecklonia cava extract solvents include water, lower alcohol (methanol, ethanol), a mixture of water and lower alcohol, acetone, ethyl acetate and 1,3- Butylene glycol aqueous solution can be used. The above-mentioned extracts are particularly suitable but not necessarily limited to the extraction solvents, and the Ecklonia cava extract can be prepared using other solvents similar in physical and chemical properties to the above-mentioned solvents. The addition amount of the extraction solvent is preferably added so as to be 1 to 20 times the weight of the Ecklonia cava. Extraction temperature and extraction time are preferably immersed at 10 to 30 ° C for 1 to 15 days. In the present invention, examples of preparing Ecklonia cava extract are shown in Examples 2 to 15 and Table 1.

As a result of culturing human fibroblasts in a medium containing the Ecklonia cava extract of the present invention, fibroblasts proliferated, and it can be seen that the Ecklonia cava extract of the present invention does not exhibit cytotoxicity and promotes fibroblast activity (Table 2). .

Ecklonia cava extract of the present invention has been found to have an effect of improving wrinkles through both in vitro and in vivo experiments. In vitro experiments were performed by measuring collagen synthesis effects using human cells, specifically human fibroblasts. Accordingly, the present invention provides a composition for promoting the synthesis of collagen, characterized in that it contains an Ecklonia cava extract and a method for promoting the synthesis of collagen by treating the composition with cells.

Fibroblasts produce extracellular matrix proteins, such as collagen, elastin, and matrix (ground substances). The cells also produce an adhesion protein that attaches epithelial cells to the basal cell membrane. Restriction of fibroblast activity limits new production of collagen, and limiting new production of collagen weakens elastin, which determines skin elasticity. The protein biosynthetic activity of such fibroblasts decreases with age (WO 92/20341).

Skin is an organ that covers the body surface of the body. The skin is largely divided into epidermis and dermis. The epidermis is the outermost part of the skin and acts as a protective barrier for internal organs. The epidermis is composed of a stratum corneum, a clear layer, a granule layer, a play layer and a base layer. The dermis serves to nourish the epidermis and to form the 'frame' of the skin within the skin tissue. The dermis is composed of a papillary layer and a deep reticular layer, which is a place where skin cell proliferation is induced by the activity of fibroblasts. The reticular layer plays a role in maintaining the elasticity of the entire skin, and collagen (collagen) and elastin (elastic fiber) are meshed (Braverman, J. Invest. Dermatol., 78, 434-443 (1982). )).

Collagen is the major matrix protein produced in fibroblasts and is present in the dermal layer of the skin. It accounts for about 70-80% of the total dry weight of the skin, and together with elastin to control the elasticity of the skin. Collagen is classified according to form and structural features, the most well known of which are Type I, Type II, Type III and Type IV. Of these, collagen type I is found in most of the skin tissues, tendons, support structures of bones and corneas of the eye. Collagen type I is particularly known for its mechanical firmness, elasticity and structural maintenance of the skin (E. U. Kucharz., Biochem. Pathophysiol., Springer Verlag, Berlin (1992)). Collagen is increased in degradation by internal factors such as decreased cellular activity due to natural aging or by external factors such as increased stress caused by various harmful environments and increased free radical species caused by sunlight (M. Dumas et al. , Mech.Aging Dev., 73, 179-187 (1994)). As collagen's biosynthesis decreases and its degradation increases, the skin loses its elasticity and firmness and wrinkles occur.

The present inventors confirmed that collagen synthesis is promoted as the concentration of the extract increases when cultured by adding the Ecklonia cava extract to the fibroblasts by concentration in vitro (Table 3). Collagen synthesis promoting effect was measured as the amount of pro-collagen type I C-peptide produced using the collagen measurement kit. Accordingly, the present invention provides a composition for promoting the synthesis of collagen and a method for promoting the synthesis of collagen by treating the composition with cells. Cells that can be used to promote the synthesis of collagen are any cells or cell lines known to synthesize collagen, preferably fibroblasts or cell lines thereof.

Thus, the compositions of the present invention can be used to treat wounds requiring synthesis of collagen and other skin conditions associated with collagen deficiency. In the case of collagen deficiency, it is known that the skin becomes less elastic, the gums collapse, and muscle pain or damage to the walls of blood vessels is caused.

The present inventors confirmed the wrinkle improvement effect of Ecklonia cava extract not only through in vitro experiments but also in vivo experiments on collagen synthesis. Wrinkle relief was measured by the replica method over 56 days in 30 women. A cream composition containing Ecklonia cava extract was prepared (Preparation A) and applied to a woman with wrinkles around the eyes. Tables 12-18).

The cosmetic composition containing the Ecklonia cava extract of the present invention is prepared according to a conventional method of manufacturing cosmetics. Ecklonia cava extract is contained in an amount of 0.001 to 20.0% by weight, preferably 0.005 to 10.0% by weight based on the dry weight of the cosmetic composition.

Cosmetic compositions containing the Ecklonia cava extract of the present invention for use on skin or hair may be used in combination with pharmaceutical or dermatologically acceptable excipients (cosmetics, creams, essences, cleansing foams, cleansing water, packs, body oils). ), Color cosmetic composition (foundation, lipstick, mascara, makeup base), hair cosmetic composition (shampoo, rinse, hair conditioner, hair gel) and the like can be applied. Excipients may include pharmaceutically acceptable emollients, skin penetration enhancers, colorants, fragrances, emulsifiers, thickening agents and solvents. The cosmetic composition which can be prepared using the Ecklonia cava extract of the present invention is described by taking a lotion, cream, solution, gel or solid as an example.

Lotion

Lotion is the middle of a lotion and cream. It is more fluid than cream and contains less oil. Its main ingredients consist of emollients, emulsifiers, thickeners, antioxidants, pH buffers and solvents.

Emollients are used to soften, calm, coat, smooth or moisturize the skin. Emollients useful in the present invention may be petroleum based, fatty acid esters, alkyl ethoxylates, fatty acid ester ethoxylates, fatty alcohols, polysiloxanes or mixtures thereof. In addition to propylene glycol, butylene glycol, glycerin, triethylene glycol, mercury, waxes, fatty acids, fatty alcohol ethers, glycerides, acetoglycerides, ethoxylated glycerides, other fatty acid esters of polyhydroxy alcohols, lanolin and derivatives thereof Such conventional emollients.

The emulsifier serves to mix the water phase and the oil phase of the cosmetic composition. Emulsifiers include both nonionic, anionic or cationic.

Thickeners include crosslinked carboxypolymethylene polymers, ethyl cellulose, polyethylene glycol, tracant gum, karaya gum, xanthan gum, bentonite, hydroxyethyl cellulose and hydroxypropyl cellulose.

Antioxidants are added to improve the stability of the cosmetic composition. Antioxidants include ascorbic acid and derivatives thereof. Preferred antioxidants include tocopherol and its derivatives.

pH buffers are used to adjust the appropriate pH of the cosmetic composition. pH buffers include acetates, phosphates, citric acid salts, triethanolamine and carbonates.

As the solvent, purified water, alcohol or a mixture of purified water and alcohol may be used.

We have prepared lotions containing Ecklonia cava extract using known methods. The lotion of the present invention contains 0.001-20% by weight of Ecklonia cava extract. The emollient was contained in an amount of 0.01-30 wt%, preferably 0.10-20.0 wt%. The emulsifier was 0.01 to 10% by weight, preferably 0.1 to 5.0% by weight. Preservatives, flavors and pigments were added in small amounts. Purified water added 70 to 90% by weight.

2. Creams

The cream of the present invention contains 0.001-20% by weight of Ecklonia cava extract. The emollient was contained 0.01 to 40% by weight, preferably 0.1 to 30% by weight. The emulsifier was to be 0.01 to 20% by weight, preferably 0.1 to 10% by weight. Purified water was added from 50 to 80% by weight. Cream preparation increased the content of oily ingredients and moisturizers as skin softeners compared to lotions.

3. Solutions Suspensions

Solutions in the cosmetic composition include lotion, essence and pack.

The lotion is generally based on purified water, ethanol and humectants. To this is added acid, alkali or astringent depending on the purpose of the lotion. The main use of the lotion is to provide sufficient moisture to the skin. In addition, it is divided into a flexible lotion and a convergent lotion according to additional purposes. A flexible lotion provides hydration and skin flexibility. Converging lotion is used for the purpose of shrinking pores.

The flexible lotion composition of the present invention contains 0.01-10.0% by weight of Ecklonia cava extract, preferably 0.1-50.0% by weight. Skin softener was added 0.1 to 10.0% by weight, preferably 0.05 to 5.0% by weight. As the solvent, 70 to 90% by weight of purified water was used.

The convergent lotion composition of the present invention contains 0.01-10.0 wt% of Ecklonia cava extract, preferably 0.05-5.0 wt%. As a solvent, 70 to 90% by weight of purified water and 1.0 to 20.0% by weight of ethanol were added.

Essence contains a high concentration of cosmetic ingredients having a skin moisturizing and anti-aging effect. Essence has the effect of moisturizing, protecting skin and nourishing. The main components of the essence are skin softeners, moisturizers, solvents, emulsifiers and thickeners.

The pack gives the skin a moderate tension and blocks the air from outside to facilitate the absorption of nutrients. It raises the temperature of the skin to promote blood circulation and removes contaminants from the surface of the skin. The main components of the pack include a film forming agent, a humectant, an emulsifier and a solvent such as ethanol.

4. Gel or solid

The gel composition can be prepared by mixing the thickener with the solution composition described above. Preferred thickening agents are as described above in the lotion composition.

The composition in solid form has use as a composition in the form of a rod for application to the lips or other parts of the body. Solid form compositions consist of emollients, thickeners, emulsifiers and solvents.

The cosmetic composition containing the Ecklonia cava extract of the present invention may be applied to a body part, particularly preferably a face, a neck, a hand, a foot, an arm, a leg, to reduce wrinkles, and the like. The cosmetic composition of the present invention can be used daily for an indefinite period of time. Preferably, the number and duration of use may be adjusted according to the age, skin condition and concentration of the Ecklonia cava extract of the user. The treatment method of the composition of the present invention can be treated by applying to the outer surface of human skin in the form of cosmetic compositions such as creams, lotions, lotions, essences and packs.

The following examples and prescription examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

Example 1

Preparation of Ecklonia cava Extract

Seaweed (1-2m in length) of seaweed, seaweed, collected from Jeju Island (near Biyangdo) was washed with purified water and dried naturally in the shade. The dried Ecklonia cava was pulverized to a size of 2-3 cm using a grinder, and then 1 Kg of these pieces were put in 9 L of water and extracted at 15 to 25 ° C. for 24 hours. The extract was filtered through Whatman 5 filter paper. The filtrate was concentrated under reduced pressure at 30 to 40 ° C. using a distillation apparatus equipped with a cooling condenser to obtain 387 g (dry weight) of Ecklonia cava extract.

Example 2

Preparation of Ecklonia cava Extract

Ecklonia cava (length 1-2m) collected from Jeju Island (near Biyangdo) was washed with purified water and dried naturally in the shade. The dried Ecklonia cava was pulverized to a size of 2-3 cm using a grinder, and then 1 Kg of these pieces were put in 9 L of 10% ethanol and extracted at 15-25 ° C. for 24 hours. The extract was filtered through Whatman 5 filter paper. The filtrate was concentrated under reduced pressure at 30 to 40 ° C. using a distillation apparatus equipped with a cooling condenser to obtain 362 g (dry weight) of Ecklonia cava extract.

Examples 3-15

Preparation of Ecklonia cava Extract

Ecklonia cava extract was prepared by the method of Example 2 using the solvent described in Table 1 below. The dry weight of the obtained extract is shown in Table 1.

Preparation of Ecklonia cava Extract According to Extraction Solvent division Solvent Dry weight (g) Example 3 20% ethanol 325 Example 4 30% ethanol 350 Example 5 40% ethanol 337 Example 6 50% ethanol 350 Example 7 60% ethanol 312 Example 8 70% ethanol 300 Example 9 80% ethanol 238 Example 10 90% ethanol 263 Example 11 100% ethanol 51 Example 12 100% methanol 178 Example 13 Acetone 14 Example 14 Ethyl acetate 10 Example 15 Butanol 15

Example 16

Measurement of Cell Proliferation Effect of Ecklonia cava Extract

Human normal fibroblasts were seeded in 96-well microplates containing DMEM medium (1 × 10 ⁴ cells / well) and 37 ° C. in a CO ₂ incubator at 5% concentration (CO ₂ ). Incubated for 24 hours. Normal human fibroblast cells were used to separate the skin tissue collected after circumcision of the newborn in the hospital. Ecklonia cava extracts prepared in Examples 1 to 15 were added to DMEM medium without serum so that the final concentration was 250 μg / ml, and the cells were further incubated for 24 hours after replacing with the medium. 10 µl of MTT solution [3- (4,5-Dimethyl-thiazole-2-yl) 2,5-diphenyl tetrazolium bromide: 5 mg / ml] was added to each well, and left for 4 hours. 100 μl of dimethyl sulfoxide (DMSO: dimethylsulfoxide) solution was added to each well, followed by stirring for 20 minutes, and the absorbance was measured at 570 nm using a microplate reader. The cell proliferation effect of Ecklonia cava extract is shown in Table 2. The cell proliferation effect was calculated by the following formula. The experimental value is the average of three experiments.

Cell Proliferation Effect of Ecklonia cava Extract According to Extraction Solvents extract Cell proliferation effect (%) Example 1 25.4 ± 1.8 Example 2 28.6 ± 2.2 Example 3 31.5 ± 1.7 Example 4 37.6 ± 1.3 Example 5 45.9 ± 2.1 Example 6 62.7 ± 3.2 Example 7 71.3 ± 3.6 Example 8 78.7 ± 2.1 Example 9 74.6 ± 3.1 Example 10 76.5 ± 2.7 Example 11 72.8 ± 2.9 Example 12 74.5 ± 3.0 Example 13 65.7 ± 2.4 Example 14 41.4 ± 1.7 Example 15 54.8 ± 1.6 The values are the average of three experimental results.

Example 17

Measurement of Collagen Synthesis Enhancement Effect of Ecklonia cava Extract

Human Normal fibroblasts as in Example 16 were seeded in 96-well microplates (2 × 10 ⁴ cells / well) and incubated at 37 ° C. for 24 hours in a CO ² incubator at 5% concentration. Incubate the Ecklonia cava extract prepared in Example 10 with 0 μg / ml, 100 μg / ml, 250 μg / ml, and 500 μg / ml, respectively, for 48 hours in DMEM medium containing no serum. It was. Ascorbic acid (50 µg / ml) was added 24 hours before the last 24 hours of culture to promote collagen synthesis. Samples were prepared by washing each well, replacing it with DM media without serum, and incubating for another 24 hours. The amount of collagen synthesized was determined using the collagen measurement kit (Procollagen Type I C-peptide EIA Kit (MK 101), Takara, Kyoto, Japan). The amount was measured. After diluting the standard solution included in the collagen measurement kit, absorbance was measured at 450 nm to prepare a standard concentration curve. 100 µl of the antibody solution containing the POD enzyme was added to each well, and 20 µl of the supernatant of the sample and the standard solution diluted for each concentration were added and mixed. The mixture was left at 37 ° C. for 3 hours, after which all contents were removed and each well was washed four times with 400 μl of PBS. 100 μl of substrate solution was added to the wells, and the mixture was left at room temperature for 15 minutes. In order to stop the reaction, 100 µl of 1N sulfuric acid solution was added and mixed, and the absorbance was measured at 450 nm. This experiment was conducted twice at the same time to find the average value. The PICP amount was calculated by substituting the absorbance of the sample in the standard concentration curve and converted into ng / 2 × 10 ⁴ cells.

As a result, Ecklonia cava extract enhanced the synthesis of collagen I in human normal fibroblasts. The results are shown in Table 3.

Enhancement of Collagen Synthesis by Ecklonia cava Extract Concentration Collagen Synthesis Amount (ng / 2 × 10 ⁴ ) Control 151 100 μg 192 250 μg 242 500 μg 287

Production Example

Cosmetic composition containing Ecklonia cava extract

A. Cream with Ecklonia cava Extract

The preparation example of the nutrient cream containing the Ecklonia cava extract prepared in Example 10 is as shown in Table 4 below. Purified water, triethanolamine, and propylene glycol which are aqueous phases are dissolved by heating at 70 ° C. The oily fatty acid, an oily component, an emulsifier, and the preservative are melt | dissolved by adding the liquid which melt | dissolved at 70 degreeC by heating. After the emulsification was completed, the solution was cooled to 45 ° C., followed by addition and dispersion of the decay extract and the flavor, followed by cooling to 30 ° C.

Ingredients and Contents of Nutritional Cream Containing Ecklonia cava Extract ingredient Content (weight%) Ecklonia cava extract 5.0 Jojoba oil 5.0 Floating paraffin 7.0 Cetylaryl alcohol 2.0 Polyglyceryl-3 Methyl Glucose Disteare 2.0 Glyceryl Stearate 0.5 Squalane 3.0 Propylene glycol 4.0 glycerin 5.0 Triethanolamine 0.3 Carboxy Vinyl Polymer 0.3 Tocopheryl acetate 0.2 antiseptic a very small amount incense a very small amount Purified water Remaining amount Sum 100

B. Lotion with Ecklonia cava Extract

a) flexible lotion

Preparation of the flexible lotion containing the Ecklonia cava extract is shown in Table 5 below. Purified water is added to the aqueous component and dissolved at room temperature to form an aqueous phase. Ecklonia cava extract, oily ingredients, emulsifiers, preservatives and flavors are dissolved at room temperature, added to the aqueous phase, mixed and filtered.

Constituents and Contents of Soft Essence Containing Ecklonia cava Extract ingredient Content (weight%) Ecklonia cava extract 2.00 EDTA 0.05 Fiji 1500 4.00 Carbomer 0.16 1,3 Butylene Glycol 3.00 Polyoxyethylene Hydrogenated Castor Oil 0.45 Triethanolamine 0.12 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount Sum 100

b) converging lotion

Examples of astringent lotion containing Ecklonia cava extract are shown in Table 6 below. Purified water is added to the aqueous component and dissolved at room temperature to form an aqueous phase. Ethanol is added to Ecklonia cava extract, oily ingredients, emulsifiers, preservatives and fragrance to dissolve at room temperature to produce an alcohol phase, which is then added to the aqueous phase, mixed and filtered.

Constituents and Contents of Converging Cosmetics Containing Ecklonia cava Extract ingredient Content (weight%) Ecklonia cava extract 2.00 glycerin 2.00 1,3 butylene glycol 3.00 EDTA 0.05 ethanol 7.00 Polyoxyethylene Hydrogenated Castor Oil 0.40 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount Sum 100

C. Lotion

Preparation of the lotion containing Ecklonia cava extract is shown in Table 7 below. Purified water, triethanol amine, and butylene glycol, which are aqueous phases, are dissolved by heating at 70 ° C. The oily fatty acid, an oily component, an emulsifier, and the preservative are melt | dissolved by adding the liquid which melt | dissolved at 70 degreeC by heating. When the emulsification is completed, it is adjusted to 0.05% by weight of the total by adding a 2% solution of xanthan gum, a hydrophilic thickener. The solution was cooled to 45 ° C., followed by addition and mixing of Ecklonia cava extract, flavor and pigment and then cooled to 30 ° C.

Ingredients and Contents of Lotion Containing Ecklonia cava Extract ingredient Content (weight%) Ecklonia cava extract 5.00 glycerin 3.00 Carbomer 0.10 Xanthan Gum 0.05 1,3 Butylene Glycol 3.00 Polyglycerin-3 Methylglucose Distearate 1.50 Glycerin Stearate 0.50 Cetearyl Alcohol 0.30 Jojoba oil 3.00 Floating paraffin 2.00 Squalane 3.00 Dimethicone 0.50 Tocopheryl Acetate 0.20 Triethanolamine 0.10 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount Sum 100

D. gel

Preparation of the gel containing Ecklonia cava extract is shown in Table 8 below.

Composition and Composition of Gels Containing Ecklonia cava Extract ingredient Content (weight%) Ecklonia cava extract 2.50 EDTA 0.02 1,3 Butylene Glycol 4.00 Carbomer 0.60 glycerin 5.00 Hydroxyethyl cellulose 0.15 Triethanol amine 0.50 Triclosan 0.20 ethanol 5.00 Polyoxy Ethylene Hydrogenated Castor Oil 4.00 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount Sum 100

E. Essence

Preparation of the essence containing the Ecklonia cava extract is shown in Table 9 below.

The thickener is added to purified water with slow stirring to disperse uniformly. The aqueous phase is mixed to prepare the aqueous phase. Ethanol is added to the emulsifier, skin softener, fragrance and preservative to dissolve. The alcohol phase is added to the aqueous phase with stirring and an alkali agent is added. Finally add Ecklonia cava extract and pigment.

Ingredients and Contents of Essence Containing Ecklonia cava Extract ingredient Content (weight%) Ecklonia cava extract 10.00 EDTA 0.05 Potassium-cetylphosphate 0.30 glycerin 5.00 1,3 Butylene Glycol 4.00 Glyceryl Stearate / Pig-100 Stearate 0.50 Sorbitan stearate 0.30 Jojoba oil 0.50 Macadamia Nut Oil 0.50 Tocopheryl Acetate 0.50 Butylated hydroxy toluene 0.05 Dimethicone 3.00 Polyacrylamide / C13-14 Isoparaffin / Laureth-7 1.50 Glyceryl Polymethacrylate / Propylene Glycol 5.00 ethanol 3.00 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount Sum 100

F. Pack

Preparation of the pack containing the Ecklonia cava extract is shown in Table 10 below.

Purified water is heated to 70 DEG C, and a film forming agent and a moisturizer are added to dissolve in a transparent manner to prepare an aqueous phase. The Ecklonia cava extract, emulsifier, preservative and fragrance are dissolved in alcohol and then mixed in the water phase and cooled to 30 ° C.

Components and Contents of Packs Containing Ecklonia cava Extract ingredient Content (weight%) Ecklonia cava extract 5.00 Benzophenone-5 0.10 1,3 butylene glycol 5.00 Carbomer 1.00 Triethanolamine 0.85 glycerin 4.00 Allantoin 0.10 DL-panthenol 0.50 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

Comparative Production Example

Cream containing purified water

The preparation example of the cream replaced with purified water without adding the Ecklonia cava extract in Preparation Example A is as follows.

Ingredients and Contents of Cream Containing Purified Water Instead of Ecklonia cava Extract ingredient Content (weight%) Jojoba oil 5.0 Floating paraffin 7.0 Cetylaryl alcohol 2.0 Polyglyceryl-3 Methyl Glucose Distearate 2.0 Glyceryl Stearate 0.5 Squalane 3.0 Propylene glycol 4.0 glycerin 5.0 Triethanolamine 0.3 Carboxy Vinyl Polymer 0.3 Tocopheryl acetate 0.2 antiseptic a very small amount incense a very small amount Purified water Remaining amount Sum 100

Example 18

Wrinkle improvement effect of cosmetics containing Ecklonia cava extract

Wrinkle improvement effect of cosmetics containing Ecklonia cava extract was measured through clinical trials. Nutritional creams prepared in Preparation Example A. (Nutrition cream containing Ecklonia cava) and Comparative Preparation Example (nutrition cream containing purified water) were used.

30 healthy women with wrinkles around the eyes (mean age 49 years: skin type III: 26 patients, II: 4 patients) were treated twice daily with morning cream and morning cream of preparation A. Apply each and measure the improvement of wrinkles. On the first day of the clinical trial, on the 28th and 56th day, a replica was made using a polymer silicone skin print on the eyes of all subjects, and then the replica was used as a skin image analyzer. Analyzed. The data obtained by analyzing the shades of gray are represented by the roughness index and the surface of the skin is represented by the numerical value. The skin wrinkles were divided into fine wrinkles (microrelief furrows up to 55 μm), medium wrinkles (depth 55-110 μm), and deep wrinkles (more than 110 μm deep). The skin area of the measurement object is 1 cm 2. The change in the degree of improvement after the measurement of wrinkles is calculated by the following equation. Data was analyzed by a pared t-test.

From here

TR: Value when using the cream of Preparation Example A

NT: Value when using the cream of the comparative preparation

Do: Before applying cream

Di: Day 28 or 56 after applying cream twice a day

The change degree of improvement is calculated by the following equation.

The skin wrinkles before and after application of the cream of Preparation Example A and Comparative Preparation Example are shown in Tables 12 to 17. The calculated value of the change of improvement was shown in Table 18. After application of the cream containing Ecklonia cava extract (Preparation Example A), the depths of the middle and deep wrinkles decreased on the 28th and 56th days.

The following shows the test results for the 28th and 56th day.

-Number of fine wrinkles: 43% and 45% of subjects increased, respectively (medium wrinkles and deep wrinkles are unfolded into fine lines)

-Number of intermediate wrinkles: 50% and 62% of subjects decreased

Deep wrinkles: 37% and 41% of subjects decreased

-Depth of deep wrinkles: 65% and 74% of subjects decreased-Total wrinkle surface: 60% and 48% of subjects decreased

Skin wrinkle degree (NT _DO ) before applying cream of comparative manufacturing example Subject Number of fine lines Fine wrinkles (μm) Number of middle wrinkles Depth of Middle Wrinkles (㎛) Number of deep wrinkles Deep Wrinkle Depth (μm) Total wrinkle area (m ² ) One 24 44.7 31 73.0 2 193.3 6.8 2 24 43.1 20 69.4 One 212.6 9.2 3 24 41.3 7 63.1 2 180.7 5.0 4 15 48.2 32 75.1 13 151.1 10.3 5 33 44.0 22 71.7 One 123.2 4.5 6 42 42.0 22 72.0 2 133.3 6.3 7 10 44.2 9 63.5 One 248.5 3.0 8 19 46.0 43 80.7 11 132.6 10.4 9 6 48.9 37 72.5 5 169.8 7.1 10 5 46.5 31 73.7 13 165.3 11.5 11 10 46.5 23 73.1 3 157.0 4.5 12 17 43.0 14 66.5 2 158.5 11.0 13 23 47.8 48 72.0 3 130.2 7.5 14 16 46.7 29 74.8 6 160.2 6.1 15 27 47.3 32 66.5 2 147.8 5.4 16 6 38.6 21 74.1 4 139.5 4.1 17 26 47.3 29 69.3 4 130.8 5.5 18 31 46.0 60 74.9 4 129.3 8.9 19 17 47.0 33 71.6 4 143.8 5.9 20 2 43.4 18 77.1 7 143.6 4.1 21 25 48.2 66 69.6 5 132.5 8.7 22 7 46.3 18 71.7 3 184.7 3.8 23 2 47.3 16 81.3 4 124.6 2.8 24 13 41.3 12 75.6 3 211.4 5.6 25 26 45.2 45 68.8 5 161.4 10.2 26 15 45.7 17 68.3 6 170.4 12.3 27 9 40.5 17 67.3 3 187.8 4.4 28 5 45.2 5 60.5 0 Of 10.0 29 14 46.3 17 66.0 4 163.7 5.8 30 18 45.6 31 75.3 10 154.3 9.6 Average 17 45.1 27 71.3 4 160.1 7.0 Median 17 45.9 23 71.8 4 157.0 6.2 Minimum 2 38.6 5 60.5 0 123.2 2.8 Maximum value 42 48.9 66 81.3 13 248.5 12.3

Skin wrinkles on day 28 after applying the cream of comparative preparation Subject Number of fine lines Fine wrinkles (μm) Number of middle wrinkles Depth of Middle Wrinkles (㎛) Number of deep wrinkles Deep Wrinkle Depth (μm) Total wrinkle area (m ² ) One 23 44.2 34 72.5 4 146.2 7.9 2 18 42.2 16 67.6 2 209.4 9.6 3 36 40.1 13 68.3 3 163.4 6.8 4 17 48.6 36 75.7 6 167.5 9.5 5 36 45.6 19 72.7 5 144.4 7.9 6 39 43.7 19 69.1 3 130.2 5.5 7 3 48.0 11 66.1 One 265.4 2.2 8 24 46.7 55 75.5 10 137.5 13.4 9 13 45.6 33 77.5 One (361.2) AV 7.0 10 13 45.9 32 76.4 13 166.0 15.0 11 14 45.2 26 76.9 3 170.9 3.9 12 21 43.3 26 72.1 3 143.7 12.5 13 22 45.5 53 71.5 2 152.6 6.8 14 7 47.7 26 73.8 7 165.4 6.5 15 15 47.4 37 72.5 2 129.8 3.6 16 11 44.3 27 74.1 4 157.3 6.4 17 19 46.1 44 73.7 6 124.7 7.3 18 13 46.5 8 71.1 3 167.1 3.1 19 25 45.3 49 71.9 3 144.5 7.4 20 10 47.6 21 69.9 9 144.7 5.6 21 67 47.7 83 68.6 10 127.9 17.1 22 15 46.9 15 74.8 5 157.9 4.6 23 6 47.6 18 77.9 10 127.0 4.7 24 5 47.1 15 76.8 2 127.2 10.0 25 40 43.7 29 68.5 3 183.3 10.1 26 12 43.7 13 78.2 5 180.7 10.7 27 20 45.3 26 77.4 6 181.7 7.4 28 4 43.0 2 73.4 0 Of 9.5 29 16 43.5 11 71.3 One 320.9 4.3 30 14 44.7 25 78.4 13 166.3 14.2 Average 19 45.4 27 73.1 5 164.4 8.0 Median 16 45.5 26 73.0 4 157.6 7.4 Minimum 3 40.1 2 66.1 0 124.7 2.2 Maximum value 67 48.6 83 78.4 13 320.9 17.1

Skin wrinkles on day 56 after applying the cream of comparative preparation Subject Number of fine lines Fine wrinkles (μm) Number of middle wrinkles Depth of Middle Wrinkles (㎛) Number of deep wrinkles Deep Wrinkle Depth (μm) Total wrinkle area (m ² ) One 23 44.9 22 72.8 6 176.1 8.4 2 9 43.1 18 73.6 5 226.6 7.5 3 27 42.9 12 70.5 3 179.1 6.2 4 17 46.8 29 69.6 6 175.2 8.3 5 25 41.5 17 67.7 One 147.7 3.6 6 29 41.7 21 71.4 2 115.2 4.4 7 11 43.6 11 65.7 One 235.2 2.8 8 17 44.3 49 72.9 10 166.1 12.5 9 17 47.2 44 75.6 7 125.3 9.3 10 9 46.2 31 76.6 9 195.5 15.2 11 19 45.1 27 76.3 2 159.8 4.4 12 8 46.3 18 75.9 2 308.2 6.9 13 12 46.4 63 77.1 11 143.4 14.6 14 17 46.3 30 69.8 6 186.3 8.1 15 33 45.2 39 70.4 2 116.6 6.4 16 11 45.7 25 78.1 10 152.3 7.4 17 20 45.3 31 72.9 0 Of 4.3 18 29 44.6 45 72.0 9 132.0 15.2 19 10 45.1 41 71.1 4 217.7 8.2 20 11 46.5 21 74.2 5 151.2 5.6 21 38 46.5 50 71.2 3 140.7 8.1 22 9 45.8 17 77.8 2 150.2 2.9 23 7 48.6 21 80.1 8 134.4 5.3 24 7 44.6 15 75.6 4 122.3 9.7 25 36 44.6 42 67.5 5 142.4 7.9 26 7 46.4 14 73.7 7 178.0 10.0 27 24 46.3 33 70.4 3 240.6 8.4 28 6 41.8 2 60.8 0 Of 9.4 29 15 46.0 16 69.8 One 290.9 4.2 30 D0 D0 D0 D0 D0 D0 D0 Average 17 45.1 28 72.5 5 174.4 7.8 Median 17 45.3 25 72.8 4 159.8 7.9 Minimum 6 41.5 2 60.8 0 115.2 2.8 Maximum value 38 48.6 63 80.1 11 308.2 15.2

Skin wrinkles before applying the cream of Preparation Example A Subject Number of fine lines Fine wrinkles (μm) Number of middle wrinkles Depth of Middle Wrinkles (㎛) Number of deep wrinkles Deep Wrinkle Depth (μm) Total wrinkle area (m ² ) One 15 46.7 19 73.8 4 179.2 7.1 2 13 43.3 5 75.1 One 120.0 1.3 3 26 41.7 32 72.7 6 173.0 9.6 4 9 46.0 22 72.2 0 Of 1.4 5 14 46.0 22 67.7 2 270.6 8.1 6 47 44.6 47 75.2 4 186.4 12.9 7 12 43.5 23 76.6 One 291.8 6.3 8 32 45.6 30 72.3 4 166.2 7.7 9 12 45.2 26 73.7 One 319.1 4.4 10 10 49.5 48 77.6 9 138.6 7.8 11 14 47.0 25 70.1 One 244.2 4.3 12 4 47.1 8 59.8 2 203.8 2.8 13 10 44.7 45 74.8 13 153.2 12.9 14 24 45.2 24 70.6 3 219.5 6.5 15 23 44.4 28 67.0 2 218.9 7.9 16 7 47.9 16 69.4 2 224.0 5.0 17 15 46.7 21 74.7 8 189.7 14.2 18 65 45.8 71 69.8 7 126.2 12.1 19 26 46.5 17 68.5 4 206.2 8.8 20 7 46.7 19 75.9 5 127.3 3.9 21 32 46.2 44 70.0 3 129.3 9.9 22 27 44.2 45 68.9 8 159.9 10.1 23 16 48.7 17 74.2 4 180.5 7.7 24 7 42.1 10 66.6 0 Of 1.2 25 28 45.6 18 64.9 4 201.9 9.6 26 10 46.9 27 74.2 10 135.3 8.1 27 4 42.7 9 69.8 3 196.6 10.1 28 9 43.2 9 62.1 One 195.6 2.5 29 35 44.5 21 68.9 3 224.4 8.5 30 54 44.4 48 70.8 6 156.7 12.4 Average 20 45.4 27 70.9 4 190.6 7.5 Median 15 45.6 23 70.7 4 188.0 7.8 Minimum 4 41.7 5 59.8 0 120.0 1.2 Maximum value 65 49.5 71 77.6 13 319.1 14.2

Skin wrinkles on day 28 after applying cream of Preparation Example A Subject Number of fine lines Fine wrinkles (μm) Number of middle wrinkles Depth of Middle Wrinkles (㎛) Number of deep wrinkles Deep Wrinkle Depth (μm) Total wrinkle area (m ² ) One 22 44.8 26 67.9 8 139.8 7.8 2 38 46.1 23 69.2 One 151.5 5.5 3 21 42.2 25 75.3 8 162.7 10.1 4 30 45.6 77 71.5 7 137.8 9.8 5 19 43.5 24 71.6 3 260.1 9.2 6 31 44.7 39 69.0 8 170.1 19.4 7 16 43.6 29 74.0 7 145.0 11.2 8 33 44.5 46 68.8 4 141.0 8.5 9 8 42.8 19 70.9 3 178.0 4.4 10 5 47.4 45 77.4 18 137.9 11.2 11 14 46.4 31 74.0 4 146.9 3.9 12 12 41.9 8 72.8 3 181.8 3.8 13 9 43.5 39 74.6 10 165.1 11.1 14 20 47.4 22 68.0 4 207.6 6.1 15 18 43.2 13 75.8 4 180.2 5.5 16 21 44.4 31 75.7 One 132.3 9.3 17 20 42.6 28 74.0 6 176.9 17.4 18 46 44.2 23 66.4 2 126.3 4.6 19 17 45.2 26 66.3 4 138.1 8.3 20 9 46.4 27 72.9 9 166.8 10.2 21 35 44.6 43 70.7 3 183.6 13.2 22 13 49.0 29 68.7 4 145.7 4.2 23 9 46.5 24 76.7 3 276.0 11.5 24 13 46.5 9 72.7 One 124.9 1.6 25 21 46.4 16 64.1 5 197.1 9.3 26 10 48.0 24 74.6 10 186.7 9.0 27 6 46.1 10 73.2 3 215.3 11.5 28 9 42.6 5 64.6 One 158.6 1.7 29 28 44.0 33 75.8 3 198.1 7.8 30 19 45.7 14 68.3 3 122.3 3.4 Average 19 45.0 27 71.5 5 168.5 8.3 Median 19 44.7 26 72.2 4 163.9 8.7 Minimum 5 41.9 5 64.1 One 122.3 1.6 Maximum value 46 49.0 77 77.4 18 276.0 19.4

Skin wrinkles on day 56 after applying cream of Preparation Example A Subject Number of fine lines Fine wrinkles (μm) Number of middle wrinkles Depth of Middle Wrinkles (㎛) Number of deep wrinkles Deep Wrinkle Depth (μm) Total wrinkle area (m ² ) One 15 40.7 26 74.3 5 166.1 8.0 2 12 44.6 21 72.3 One 138.6 2.6 3 25 45.1 35 73.2 10 139.8 11.1 4 34 46.3 75 71.9 6 133.1 10.7 5 14 41.4 19 67.4 3 230.0 8.2 6 59 44.2 30 70.3 4 122.1 10.3 7 14 43.9 18 76.8 4 186.4 7.6 8 28 46.9 44 70.8 8 173.5 12.1 9 14 46.0 15 64.1 0 Of 1.9 10 13 49.8 38 80.3 12 149.5 11.3 11 8 46.7 21 75.1 One 112.9 2.1 12 4 46.3 15 72.3 2 157.2 2.8 13 8 42.1 46 74.4 14 142.8 9.2 14 20 44.8 24 69.1 0 Of 6.2 15 20 45.2 30 69.3 3 243.5 10.8 16 8 46.4 14 75.6 One 122.7 6.4 17 16 42.5 27 71.9 One 148.8 11.1 18 57 43.3 30 62.3 One 123.1 6.7 19 15 43.9 17 67.5 5 157.3 9.4 20 16 43.7 14 71.4 4 202.1 8.6 21 52 43.1 33 66.7 3 139.6 9.5 22 31 47.2 45 73.3 6 161.9 11.2 23 7 50.2 18 74.9 5 165.2 7.7 24 10 45.4 7 59.2 0 Of 0.9 25 23 44.5 20 72.0 5 126.7 6.3 26 11 47.5 21 78.4 6 142.0 5.8 27 8 42.1 12 66.8 4 211.1 9.0 28 9 43.2 7 (84.2) AV 2 183.5 2.5 29 33 42.1 17 65.8 2 145.4 9.1 30 D0 D0 D0 D0 D0 D0 D0 Average 20 44.8 25 71.0 4 158.6 7.5 Median 15 44.6 21 71.9 4 149.2 8.2 Minimum 4 40.7 7 59.2 0 112.9 0.9 Maximum value 59 50.2 75 80.3 14 243.5 12.1

Skin wrinkle improvement division D28 D56 △% (TR / NT) △ (TR / NT) P △% (TR / NT) △ (TR / NT) P Number of fine lines -15% -3 3 0.223 + 4% +1 2 0.703 Depth of Fine Lines (㎛) -1.6% -0.7 0.5 0.201 -1.5% -0.7 0.6 0.247 Number of medium wrinkles -One% 0 3 0.951 -5% -1 3 0.622 Depth of intermediate wrinkles (㎛) -1.7% -1.3 0.9 0.148 -2.2% -1.6 0.9 0.096 Number of deep wrinkles + 13% +1 1 0.499 -7% 0 1 0.747 Deep wrinkle depth (㎛) -11.2% -21.8 12.5 0.093 -21.9% -44.9 14.9 0.006 Total wrinkle area (mm ² ) -1.9% -0.2 0.8 0.836 -7.7% -0.6 0.8 0.434

According to the present invention, the extract extracted from Ecklonia cava, which is a kind of seaweed inhabiting the waters of Korea, can be substituted for the wrinkle improvement raw materials currently used in the cosmetics industry as the result of clinical trials proves excellent wrinkle improvement effect. It can be expected to improve the international competitiveness of Korea's international competitiveness and increase the utility and added value of domestic wild algae.

Claims (7)

delete delete Collagen synthesis promoting composition comprising an extract of Ecklonia cava The composition for promoting collagen synthesis according to claim 3, wherein the Ecklonia cava extract is contained in an amount of 0.001 to 20.0% (w / w) based on the dry weight of the composition. Hair composition comprising an Ecklonia cava extract. delete The Ecklonia cava extract according to claim 3 or 4, wherein the Ecklonia cava is extracted by immersing it in water, anhydrous or hydrous lower alcohol, acetone, ethyl acetate, butanol for 12 to 24 hours at 15 to 25 ° C. Collagen synthesis promoting composition comprising.