KR101727313B1 - The hieracium umbellatum extracts by ultrasonic extraction and its manufacturing method and the hieracium umbellatum extraction as active ingredient cosmetic composition for improving of an antioxidant, anti-inflammatory, atopic skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving antioxidant, antiinflammation and atopic skin using an extract of Aspergillus oryzae by ultrasonication, a method for producing the same, and an extract of Aspergillus oryzae as an active ingredient. , And extracts the extracts of Araliaceae, which are excellent in antioxidant, anti-inflammatory and atopic skin improving effects and have no side effects on skin irritation.
In addition, by providing a cosmetic composition using a crude extract of Aspergillus oryzae, antioxidative, antioxidant, and anti-inflammatory agents that inhibit the activity of lipoxygenase and hyaruronidase and eliminate free radicals, And to cosmetic compositions for improving anti-inflammatory and atopic skin.
The present invention relates to a method for producing a crude egg product, comprising the steps of (T1) mixing a crude egg product with water, ethanol, or 1,3-butylene glycol, followed by immersion to obtain a crude egg product deposit (T1) And extracting the crude extract of the present invention (step S2). The method of the present invention is characterized in that it is a method for producing the crude extract of the present invention by the ultrasonic extraction method.
The present invention also relates to a method for producing a crude egg product, comprising the steps of (S1) mixing a crude egg product with water, ethanol, and 1,3-butylene glycol mixed solvent and then immersing the mixture into a crude egg product deposit (S1) And obtaining a crude extract of the present invention (S2).
In addition, the present invention is characterized in that in step (T1) or step (S1), the crude oil and the solvent are mixed, and then the mixture is immersed at 4 to 50 ° C for 1 to 24 hours to obtain a crude oil deposit.
In addition, the present invention is characterized in that the method further comprises a step (S3) of cooling, filtering and concentrating the crude extract of the present invention obtained in the step (S2).
In addition, in the present invention, the crude extract of the present invention has an effect of suppressing the activity of lipoxygenase and hyaruronidase and eliminating free radicals, and has an effect of being selected for antioxidation, anti-inflammation and improvement of atopic skin, It has an effect of not having the effect of the present invention.
In addition, the present invention is characterized in that it is a cosmetic composition for improving antioxidant, anti-inflammatory, and atopic skin comprising an extract of Aspergillus oryzae as an active ingredient.
In addition, the cosmetic composition of the present invention is characterized by containing 0.01 to 10% by weight of liquid crude herb extract as a total weight.
In addition, the cosmetic composition of the present invention is characterized in that it contains 0.001 to 5% by weight of powdery crude extract according to the total weight.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving antioxidant, antiinflammatory and atopic skin using an extract of Aspergillus oryzae by ultrasonic extraction, a method for producing the same, and a crude extract of Aspergillus oryzae as an active ingredient. ingredient cosmetic composition for improving an antioxidant, anti-inflammatory, atopic skin}

The present invention relates to a cosmetic composition for improving antioxidant, antiinflammation and atopic skin using an extract of Aspergillus oryzae by ultrasonication, a method for producing the same, and an extract of Aspergillus oryzae as an active ingredient. , And extracts the extracts of Araliaceae, which are excellent in antioxidant, anti-inflammatory and atopic skin improving effects and have no side effects on skin irritation.

In addition, by providing a cosmetic composition using a crude extract of Aspergillus oryzae, antioxidative, antioxidant, and anti-inflammatory agents that inhibit the activity of lipoxygenase and hyaruronidase and eliminate free radicals, And to cosmetic compositions for improving anti-inflammatory and atopic skin.

Skin is a part of the body that is directly exposed to the external environment. Exposure to excessive ultraviolet rays or pollutants causes skin irritation such as erythema, edema, itching, and inflammatory reaction. Such skin troubles are not only cosmetic problems, but also substances which are produced in the inflammatory reaction cause collateral pigmentation, promote skin collapse of elastic fibers, and increase skin wrinkles. In addition, skin damage is known to be associated with free radicals.

The theory of free radicals was presented by D. Harman in the mid-1950s, and has recently gained attention in the academia and related industries. Free radicals cause various problems such as wrinkle formation, skin cancer, cell killing, rheumatoid arthritis, atopic dermatitis, acne and the like because it destroys cells or cuts or cross-links connective tissue of skin dermal layer.

The cause of free radicals is known as the action of white blood cells, the action of electron transport system, myeloperoxide (MPO) during the production of ATP in mitochondria, ultraviolet rays, tobacco, normal metabolic processes, stress, pollutants and bacteria have.

The above-mentioned phagocytosis refers to the action of the cell body to take foreign matter or bacteria into the body and decompose and detoxify the body.

Of course, the body has superoxide dismutase (SOD), catalase, vitamin E, vitamin C and ubiquinol, which are antioxidants (radical scavenging agents), but the antioxidant system by age, pollution, And the free radicals gradually increase.

The increased free radicals break collagen, elastin and hyaluronic acid, which are connective tissues of the dermis, causing settlement of the skin (wrinkles) in certain areas of the skin and also oxidizing the lipid part of the cell membrane, Destruction phenomenon occurs, and it causes diseases of dermatitis, acne and skin cancer.

The free radicals are involved in a spontaneous oxidation reaction (dopaquinone → melanin) during melanin formation, and cause free radicals and cause wrinkles. (Journal of the Korean Society of Cosmetic Scientists, Vol. 23, No. 1, pp. 132-132)

Inflammation is a physiological response that protects the body from harmful environmental conditions, such as intrusion of external foreign matter such as bacteria and mechanical damage. This inflammation results in a large number of polynuclear leukocytes (PMNs) and immune substances, and these cells secrete a variety of inflammatory cell products, proteases and cytokines, I will. Enzymes such as Elastase, hyalurinidase and lipoxygenase are well known as proteins and lipolytic enzymes produced during inflammation.

On the other hand, these actions can cause harmful damage to adjacent tissue cells and non-tissue cell components, resulting in severe tissue damage of chronic inflammation.

Such excessive inflammation damages cells and connective tissues by proteolytic enzymes, and damage of connective tissues not only causes reduction of skin elasticity to cause wrinkles but also adversely affects cell regeneration and proliferation, Resulting in aging of the skin and localized erythema and swelling in the skin. If the connective tissue is severely damaged and the inflammatory response persists, restoring normal tissue structure and function becomes much more difficult.

The generation of mediators that promote leukocyte levels along with inflammation is also mediated by the process through arachidonic acid. This pathway is a process by cyclooxygenase and a process by lipoxygenase. Leukotriene and prostagladine produced by these enzymes act as an inflammation inducer. Thus, lipoxygenase and cyclooxygenase inhibitors inhibit the production of inflammatory substances, as well as inhibit the cell membrane breakage and lipid peroxidation by free radicals produced during inflammation, and exhibit anti-inflammatory action . In addition, it inhibits proteins and lipolytic enzymes produced by excessive inflammation, thereby protecting cells damaged thereby, thereby inhibiting skin aging and improving atopic skin.

As an example of a conventional technique for inhibiting the generation of free radicals, see J. Korean Soc Food Sci Nutr. 39 (2). The antioxidative effect of ethanol extracts of Asteraceae in Korea is reported to be related to the contents of total polyphenols and total flavonoids, DPPH and ABTS radical scavenging ability, Fe ^{2 +} chelating effect, The antioxidant activity of linoleic acid was examined and its antioxidative effect was confirmed in a plant extract of Asteraceae.

The method of the above-mentioned prior art is a method for producing a plant of the present invention, comprising the steps of washing a plant of Asteraceae immediately after harvesting, lyophilizing and pulverizing the plant, pulverizing the dried sample with 80% ethanol at reflux for 6 hours at 60 ° C, ; And after filtration, the residue is extracted twice and repeatedly extracted three times in total.

Although the extract of Asteraceae which is extracted by the above-mentioned production method of the prior art can obtain an extract having an antioxidative effect, the antioxidative effect varies depending on the plant species. In the case of the above production method, the amount of the extract having antioxidative activity .

In addition, there is a disadvantage in that heat is applied directly to a plant of Asteraceae to cause destruction and loss of many active ingredients due to heat, and instability of various useful ingredients.

Accordingly, there is a need for a method capable of minimizing the component destruction of various useful components present in the fruits of natural plants and extracting them effectively.

In other words, in order to reduce skin irritation and inflammation caused by various stress sources, and to reduce adverse effects caused by cosmetic use, it is urgently required to develop a substance having anti-inflammatory, antioxidant and skin irritation mitigating effect. There is also a need for a method for effectively extracting less of the natural component of the useful component while minimizing the destruction of the component.

Therefore, in order to improve the skin trouble caused by the free radicals and inflammation, it is necessary to develop a natural extract having excellent antioxidative, anti-inflammatory and atopic skin improving effects and no side effects, to develop a cosmetic containing the extract, Development is essential.

The present invention has been conceived in order to solve the above problems, and it is an object of the present invention to provide a method for inhibiting the activity of lipoxygenase and hyaruronidase and eliminating free radicals, It is an object of the present invention to provide an extract of Araliaceae which has an effect to be selected for the improvement of atopic skin and has no adverse effect on skin irritation.

In order to improve the disadvantages of the conventional hot water extraction method in which the amount of the extract having antioxidant activity is small and heat is applied to the raw material to cause destruction and loss of many active ingredients due to heat, The present invention also provides a method for preparing a crude extract of the present invention by immersing it in a mixed solvent and then subjecting it to ultrasonic extraction.

In addition, the present invention provides a cosmetic composition for improving antioxidant, anti-inflammation and atopic skin by using a crude extract of Aspergillus oryzae to inhibit the activity of lipoxygenase and hyaruronidase and free radicals, And a cosmetic composition for improving antioxidant, antiinflammation and atopic skin using the extract of Aspergillus oryzae as an active ingredient.

The present invention relates to a method for producing a crude egg product, comprising the steps of (T1) mixing a crude egg product with water, ethanol, or 1,3-butylene glycol, followed by immersion to obtain a crude egg product deposit (T1) And extracting the crude extract of the present invention (step S2). The method of the present invention is characterized in that it is a method for producing the crude extract of the present invention by the ultrasonic extraction method.

The present invention also relates to a method for producing a crude egg product, comprising the steps of (S1) mixing a crude egg product with water, ethanol, and 1,3-butylene glycol mixed solvent and then immersing the mixture into a crude egg product deposit (S1) And obtaining a crude extract of the present invention (S2).

In addition, the present invention is characterized in that in step (T1) or step (S1), the crude oil and the solvent are mixed, and then the mixture is immersed at 4 to 50 ° C for 1 to 24 hours to obtain a crude oil deposit.

In addition, the present invention is characterized in that the method further comprises a step (S3) of cooling, filtering and concentrating the crude extract of the present invention obtained in the step (S2).

In addition, in the present invention, the crude extract of the present invention has an effect of suppressing the activity of lipoxygenase and hyaruronidase and eliminating free radicals, and has an effect of being selected for antioxidation, anti-inflammation and improvement of atopic skin, It has an effect of not having the effect of the present invention.

In addition, the present invention is characterized in that it is a cosmetic composition for improving antioxidant, anti-inflammatory, and atopic skin comprising an extract of Aspergillus oryzae as an active ingredient.

In addition, the cosmetic composition of the present invention is characterized by containing 0.01 to 10% by weight of liquid crude herb extract as a total weight.

In addition, the cosmetic composition of the present invention is characterized in that it contains 0.001 to 5% by weight of powdery crude extract according to the total weight.

As described above, the crude extract of the present invention has an effect of inhibiting the activity of lipoxygenase and hyaruronidase and eliminating free radicals, and has an effect of being selected for antioxidation, anti-inflammation and atopic skin improvement, There is no side effect.

In order to improve the disadvantages of the conventional hot water extraction method in which the amount of the extract having antioxidant activity is small and heat is applied to the raw material to cause destruction and loss of many active ingredients due to heat, The present invention provides a method for preparing a crude extract of the present invention by immersing it in a mixed solvent and then subjecting it to ultrasonic extraction.

In addition, the present invention is effective in inhibiting the activity of lipoxygenase and hyaruronidase and eliminating free radicals, thereby improving antioxidant, antiinflammation, atopic skin, and providing a cosmetic composition free from adverse effects on skin irritation.

FIG. 1 is a flow chart showing a method for preparing a crude extract according to an embodiment of the present invention by an ultrasonic extraction method.

The present invention studies skin defense mechanisms against oxidative, inflammatory and atopic symptoms based on a herbal plant. As a result, the present inventors completed the present invention by confirming that the crude extract extracted with the ultrasonic extraction method is effective in improving antioxidation, antiinflammation and atopic skin.

FIG. 1 is a flowchart showing a method for preparing a crude extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for producing a crude egg product, comprising the steps of: (T1) mixing a crude egg product with water or a solvent of ethanol or 1,3-butylene glycol, and then immersing the mixture at 4 to 50 ° C for 1 to 24 hours to obtain a pre- (2) a step of putting the deposits of the raw ornamental herbs in an ultrasonic wave extractor and irradiating the ultrasonic waves with an ultrasonic wave to obtain a crude extract of the raw rice (Step S2).

The present invention relates to a method for producing a crude egg product by mixing a crude egg product with water, ethanol and 1,3-butylene glycol mixed solvent, and then immersing the mixture at 4 to 50 ° C for 1 to 24 hours to obtain a crude egg product deposit (S1) (Step S2) of adding the extract to an ultrasound extractor and irradiating it with ultrasonic waves to obtain a crude extract of the present invention.

Generally, ultrasonic waves refer to sound waves (sound waves) having frequencies higher than the range of 16 Hz to 16 KHz (> 16 KHz), which is the human audible frequency range. When the ultrasonic wave is generated, the cavitation generated when the ultrasonic wave passes through the medium affects the chemical reaction, so that the chemical reaction can be promoted. The energy source that improves the reactivity when using ultrasonic waves in various chemical reactions is a cavitation phenomenon, and cavitation proceeds in three steps, namely, nucleation, bubble growth, and explosive rupture of fully grown bubbles.

When ultrasonic energy is used for extraction, very large energy is generated by cavitation by ultrasonic vibration. In addition, due to the high local temperature, the kinetic energy of the reactant particles located in the periphery is increased, so that sufficient energy is obtained for the reaction, and the shock effect of the ultrasonic energy induces a high pressure to increase the mixing effect.

Ultrasonic extraction is completed in a very short time compared with solvent extraction and extraction time is shortened to about 10 times that of supercritical fluid extraction process. It is thought that this is because, in the ultrasonic extraction, the internal tissue of the cell is broken due to the high pressure caused by the joint effect of the ultrasonic waves, the moving distance of the lipid is shortened and the diffusion easily occurs.

In the present invention, the step (T1) of mixing the crude oil and the mixed solvent and obtaining the crude oil or salt deposit by immersing the crude oil in the mixture is carried out by mixing the crude oil with at least one selected from the group consisting of water or an alcohol having 1 to 4 carbon atoms or a glycol solvent having 1 to 4 carbon atoms After immersing in a solvent at 4 to 50 DEG C for 1 to 24 hours, ultrasonic extraction is performed.

In the step (S1) of mixing the cooked ornamental herbs and the mixed solvent and obtaining the cooked herb seeds by immersion, the mixture of the cooked herbs and the mixed solvent has a mixing ratio of 1: 1 to 1:10 parts by weight, Is dissolved in at least one extraction solvent selected from the group consisting of alcohols having 1 to 4 carbon atoms, glycols having 1 to 4 carbon atoms and mixed solvents thereof at 4 to 50 DEG C for 1 to 24 hours, followed by ultrasonic extraction.

The alcohol having 1 to 4 carbon atoms is preferably methanol, ethanol, n-propanol, iso-propanol, n-butanol, iso-butanol or tert-butanol, more preferably ethanol, and the glycol is 1,3- , Propylene glycol, ethylene glycol, and preferably 1,3-butylene glycol. More preferably, a mixed solvent of ethanol, 1,3-butylene glycol and water may be used.

In the present invention, the step (S2) of injecting the crude herb deposit in the ultrasonic extractor and irradiating the ultrasonic wave with the ultrasonic wave to obtain the crude extract of the present invention is performed by putting the crude egg material deposit obtained in the above step (S1 or T1) into an ultrasonic wave extractor, Adjust the position so that it can be delivered and operate by ultrasonic extraction.

The ultrasonic waves are extracted using an ultrasonic wave of 20 to 60 KHz using a 100 to 700 watt output. 200 to 400 watt output is preferably used for ultrasonic waves of 20 to 60 KHz. The output range is an optimum range to prevent the tissue of the cooked rice product from being crushed to form a precipitate.

The temperature during the ultrasonic extraction is preferably maintained at 4 to 50 ° C., preferably at 15 to 40 ° C., for 20 to 20 hours, and then repeatedly extracted 1 to 3 times. The temperature range is the optimum range to maintain the shape of the cooked rice without boiling.

The present invention is further characterized in that the method further comprises a step (S3) of cooling, filtering, and concentrating the crude extract of the present invention obtained in the step (S2).

The step (S3) of cooling the crude extract obtained in step (S2) and filtering and concentrating the cooled extract is performed by cooling with a cooling condenser in order to prevent evaporation of the solvent after extraction by ultrasonic wave in step (S2) , And finally, concentrating the extraction solvent with a vacuum concentrator. Filtered through a filter having a particle size of 0.2 to 2 占 퐉, and concentrated using a vacuum concentrator.

Not only is it possible to efficiently obtain an effective ingredient through the ultrasonic extraction at a low cost, but also each effective ingredient can be effectively expressed after extraction and synergistic effect can be obtained.

In the present invention, the crude extract of the present invention has the effect of inhibiting the activity of lipoxygenase and hyaruronidase and eliminating free radicals, and has an effect of being selected for antioxidation, antiinflammation and improvement of atopic skin, and has no side effect on skin irritation .

The crude extract has excellent free radical scavenging, antiinflammatory and atopic skin improving effect and is free from side effects on skin irritation. The crude extract of the present invention may be in the form of a concentrated liquid or a dried powder.

The above-mentioned Chinese herb ( Hieraci μm ㎛ bellatum L.) is a plant of Compositae and is a perennial plant having a height of 60 to 90 cm. Leaves are willow-like and serrated. Yellow flowers bloom in summer. It grows in mountains and fields of various places. Ingredients include inulin, tanninol, and ascorbic acid in the leaves. Cryopresenting in the private sector, coughing, pulmonary tuberculosis, epidermis, rhododendron, rhododendron, astringent python, aphrodisiac, and many skin diseases. Young leaves eat wild plants. It is called the mountain bureau, and it is used in the Chinese medicine to treat the tongue. (Ingredients and use of herbs, edited by Encyclopedia of Science Publishing Company, Jan., 1994, Chinese Medicine Dictionary, Chinese Medicine Dictionary Compilation Committee,

The present invention is characterized in that it is a cosmetic composition for improving antioxidation, anti-inflammation, and atopic skin comprising an extract of Aspergillus oryzae as an active ingredient.

The cosmetic composition for improving antioxidation, antiinflammation, and atopic skin comprising the extract of Aspergillus oryzae as an active ingredient exhibits excellent anti-inflammatory and antioxidant effects and exhibits excellent stability in the products to be applied. In addition, it has the effect of inhibiting the activity of lipoxygenase and hyaruronidase, and eliminating free radicals, and has a skin irritation and inflammation relieving effect.

The cosmetic composition according to the present invention is characterized by comprising a crude extract of Araliaceae and comprises 0.01 to 10% by weight, based on the total weight of the cosmetic composition, the liquid Araliaceae extract obtained in the step (S2) % Of liquid crude herb extract.

Alternatively, the crude extract may be pulverized using a distillation apparatus equipped with a cooling condenser, wherein the cosmetic composition contains 0.001 to 5% by weight of powdery crude extract as a dry weight of the powdery crude extract, And 0.01 to 3% by weight of powdered spinach herb extract.

In the case of the liquid crude aspartame extract and the powdery crude herb extract, inhibition of the activity of lipoxygenase and hyaruronidase and elimination of free radicals are shown when the concentration is less than 0.01 wt% (liquid phase) and 0.001 wt% (powder) There is a problem that when it is 10 wt% (liquid) and 5 wt% (powder), itchy and tingling occurs to people having dry skin.

The components contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the active ingredient, crude extracts, and may be used in conventional cosmetic compositions such as antioxidants, stabilizers, solubilizers, vitamins, pigments, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, a pack, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, at least one of animal oil, vegetable oil, wax, paraffin, starch, trachant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide Can be selected and used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Particularly in the case of spraying, chlorofluorohydrocarbons, propane / Propane, such as butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycerol, aliphatic esters, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquiolead, paraffin, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, Sorbitan stearate, glycerin monostearate, stearic acid, glyceryl stearate / fat-400 stearate, carboxy polymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, , Macadamia oil, carboxyvinyl polymer, xanthan gum or sorbitan fatty acid Esters can be selected and used.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol, glycerin, butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, Such as, for example, suspensions, microcrystalline cellulose, hydroxyethylcellulose, sodium hyaruronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate , Sorbitan sesquioleate, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate, wax, paraffin, squalane, caprylic / capric triglyceride, carboxyvinyl polymer, triethanolamine, Select one or more of Kant Can.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide At least one selected from ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

Hereinafter, the present invention will be described in detail by Examples, Comparative Examples, Experimental Examples and Formulation Examples.

The following Examples, Comparative Examples, Experimental Examples and Formulation Examples are merely illustrative of the present invention, and the present invention is not limited to the following Examples, Comparative Examples, Experimental Examples and Formulation Examples.

< Example 1> Preparation of a crude extract of Araliaceae by mixing the crude Aralia herb with water, ethanol and 1,3- butylene glycol mixed solvent to obtain a crude Aralia sp.

1,000 g of dry crude ash was placed in 10,000 g of a mixed solvent containing 6,000 g of purified water, 1,000 g of ethanol and 3,000 g of 1,3-butylene glycol, and the mixture was immersed for about 3 hours. The crude herbs were collected from Taebaek city, Gangwon province. The above mixture was put into an ultrasonic wave extracting machine (manufactured by Dominant Ultrasonic wave) and ultrasonic wave extraction was performed. The temperature of the extractor was kept at 30 ℃ and the output of the ultrasonic wave was increased to 200 watt for about 2 hours. The crude herbs which were wrapped in hemp cloth were removed and the extract was filtered with a 0.8 ㎛ filter to obtain a crude herb extract. The filtered crude herb extract was left at room temperature for one week, filtered through a 0.45 mu m filter, and then completely concentrated at 50 DEG C using a vacuum concentrator to obtain 139.62 g of dry weight.

<Example 2> jobap herbs jobap the herbs are mixed, and immersed in water, solvent Deposits Preparation of crude extracts of crude ash obtained by ultrasonic extraction of crude extract

Using the solvent as the water, the crude extract was prepared in the same manner as in Example 1 to obtain a dry weight of 107.38 g.

<Example 3> a mixture of herbs jobap in ethanol solvent, and immersed jobap herbs Preparation of crude extracts of crude aspartame extracts obtained by ultrasonication after extracting deposits

The crude extract was prepared in the same manner as in Example 1, using ethanol as the solvent, to obtain 117.69 g of a dry weight.

<Example 4> Preparation of jobap herb extract extracted by a mixture, and after immersing the herbs in jobap 1,3-butylene glycol solvent, 30% obtained for the deposit jobap herbs jobap herb extract ultrasonic extraction

The crude extract was prepared in the same manner as in Example 1, except that the solvent used was a 30% aqueous solution of 1,3-butylene glycol. A dried weight of 109.21 g was obtained.

< Example 5> Preparation of a crude extract of Araliaceae by the ultrasonic extraction method after mixing crude Araliaceae with a 30% ethanol aqueous solution and obtaining the crude Aralia sp.

The crude extract was prepared in the same manner as in Example 1 except that the solvent was changed to 30% ethanol aqueous solution to obtain 115.11 g of dry weight.

<Comparative Example 1> Preparation of the jobap herbs with water, ethanol and 1,3-butylene glycol jobap herb extract extracted by the heat extraction are mixed in a mixed solvent

1,000 g of dry crude ash was placed in 10,000 g of a mixed solvent containing 6,000 g of purified water, 1,000 g of ethanol and 3,000 g of 1,3-butylene glycol, and the mixture was boiled at 100 ° C for 5 hours in an extractor equipped with a cooling condenser After removing the medicinal material, it was filtered with a 300 mesh filter, left for 1 week, and the precipitate was filtered with a 0.45 mu m filter. The mixture was completely concentrated at 50 ° C using a vacuum concentrator to obtain 103.52 g of a dry weight. The crude herbs were collected from Taebaek city, Gangwon province.

& Lt; Comparative Example 2 > Preparation of crude extract of crude rice , which was prepared by mixing the crude rice oil with water and extracting it by the heat extraction method

Using the solvent as the water, the crude extract was prepared in the same manner as in Comparative Example 1 to obtain 79.23 g of dry weight.

& Lt; Comparative Example 3 > Preparation of a crude extract of Bombyx mori Extract, which was prepared by mixing hot broth with an ethanol solvent and extracting it by a heat extraction method

The crude extract was prepared in the same manner as in Comparative Example 1, using ethanol as the solvent, to obtain 95.72 g of a dry weight.

& Lt; Comparative Example 4 > Preparation of a crude extract of Bombyx mori Extract, which was prepared by mixing the crude rice bran with 30% of 1,3- butylene glycol solvent and extracting it by the heat extraction method

The crude extract was prepared in the same manner as in Comparative Example 1, except that the solvent used was a 30% aqueous solution of 1,3-butylene glycol, and 84.34 g of dry weight was obtained.

& Lt; Comparative Example 5 > Preparation of a crude extract of Bombyx mori Extract, which was prepared by mixing rice bran with 30% ethanol and extracting it by the heat extraction method

Using a 30% ethanol aqueous solution as a solvent, a crude extract was prepared in the same manner as in Comparative Example 1 to obtain a dry weight of 90.22 g.

< Experimental Example  1> Sweet pepper  Identification of cell proliferation and toxicity by extract

The effect of the dried powder of the crude extract prepared from Examples 1 to 5 and Comparative Examples 1 to 5 on cell proliferation was confirmed.

Experiments were carried out in the following manner to confirm cell proliferation and toxicity.

The cells were cultured in a 96-well microplate at 5,000 cells / well in a thermostatic chamber for 30 minutes. The samples were treated with 0.01, 0.05, 0.1 and 0.5% (w / w) V)) and cultured for 72 hours. After 72 hours of incubation, thiazoline blue was added and further incubated for 4 hours. The culture medium was discarded and the reaction stop solution was added to each well of the microplate. After stirring for 5 minutes, the absorbance at 570 nm was measured. The control group was co-cultured with 10% Fetal Bovine Serum (FBS) medium as an optimal condition for cell growth, and the cell proliferation rate of the test group was calculated with the cell proliferation of the control group as 100% Respectively.

The cell proliferation effect was calculated by Equation 1, and the results are shown in Table 1 below.

<Formula 1>

Cell proliferation and toxicity reduction effect by extract density
(%) Cell proliferation effect (%) Example Comparative Example One 2 3 4 5 One 2 3 4 5 Control group 0 0 0 0 0 0 0 0 0 0 0.01 7.8 6.5 5.6 5 5.8 2.8 2.2 1.3 1.2 1.6 0.05 13.2 10.2 9.7 7.9 9.7 6.1 5.7 4.2 4.5 5.1 0.1 20.8 17.3 15.8 14.3 16.1 12.6 10.7 9.8 8.9 10.3 0.5 28.6 23.2 19.7 17.6 20.7 17.1 13.9 11.5 10.8 14.3

As shown in Table 1, when the cell proliferation at the optimal cell growth conditions was taken as 100%, the crude extract of Examples 1 to 5 and Comparative Examples 1 to 5 had no cytotoxicity, Cell proliferative effect was confirmed. Particularly, Examples 1 to 5 showed superior cell proliferation effect than Comparative Examples 1 to 5, and the extract of Example 1 showed the best cell proliferation effect.

< Experimental Example  2> Sweet pepper  By extract Free radical  Verify erase effect

The effects of free radical scavenging on the dry powder of the crude extract prepared from Examples 1 to 5 and Comparative Examples 1 to 5 were confirmed.

Experiments were carried out in the following manner to confirm the effect on free radical erase.

Experiments were carried out using the DPPH (1,1-diphenyl-2-picryl-hydrazyl) method (Blois, MSNature 181, 1190, 1958) and DPPH and quercetin were used from Sigma . To 1 ml of a 0.2 mM DPPH methanol solution, various concentrations (5, 10, 20, and 30 ppm) of Examples 1 to 5 and 2 ml of the solutions of Comparative Examples 1 to 5 were added and stirred, followed by reaction at room temperature for 10 minutes. Thereafter, the absorbance was measured at 517 nm, and purified water was used instead of each sample in the blank test.

The change of the free radicals was measured using the following equation 2 and the results are shown in Table 2.

<Formula 2>

Effect of Free Radical Scavenging on Extracts of Herb Extracts Free radical scavenging effect (%) density Example Comparative Example (ppm) One 2 3 4 5 One 2 3 4 5 0 0 0 0 0 0 0 0 0 0 0 5 13.7 9.8 11.9 9.7 11.5 6.2 4.5 6 4.8 6.3 10 48.6 38.8 40.2 44.1 43.9 29.6 18.5 25.7 20.3 28.1 20 88.7 82.5 86.3 84.1 85.9 68.2 42.7 51.9 48.6 58.1 30 98.8 96.7 98.3 96.2 98.3 89.3 77.6 80.8 86.2 88.9 SC50 13.01 14.27 13.75 13.92 13.59 18.00 21.42 19.91 20.18 18.77

As shown in Table 2, it was confirmed that the free radical scavenging effects of Examples 1 to 5, which were extracted from the crude extracts by ultrasonic extraction, were superior to the free radical scavenging effects of the crude extracts prepared in Comparative Examples 1 to 5. In particular, it was confirmed that the effect of Example 1 was better than that of the crude extract of Examples 2 to 5 and Comparative Examples 1 to 5.

< Experimental Example  3> Sweet pepper  By extract Lipoxygenase  Identification of the activity inhibition effect

The effect of inhibiting the activity of Lpoxygenase was confirmed by using the dried powder of the crude extract of the present invention prepared in Examples 1 to 5 and Comparative Examples 1 to 5.

In order to confirm the effect on inhibition of Lpoxygenase activity, experiments were carried out in the following manner.

Experiments were performed using the TBAS method. Linoleic acid, Lipoxygenase, and Thiobabitulic acid used in the experiments were those of Sigma (Sigma). 0.05 ml of each of Examples 1 to 5 and Comparative Examples 1 to 5 at various concentrations (5, 10, 20, 30, and 40 ppm) were added to 1 ml of 1 mM linoleic acid and 0.95 ml of Lipoxygenase was added. Minute reaction. After adding 0.5 ml of trichloroacetic acid and 1 ml of thiobarbituric acid, the reaction is terminated by heating for 10 minutes and cooling in ice water for 2 to 3 minutes. 2 ml of butanol was added to the reaction solution, and the solution was centrifuged at 4,000 g for 5 minutes. Then, the absorbance was measured at 535 nm, and purified water was used instead of each sample for the blank test.

The inhibitory effect of Lipoxygenase activity was determined using the following equation 3, and the results are shown in Table 3 below.

 <Expression 3>

Inhibition of lipoxygenase activity by the extract Lipoxygenase activity inhibitory effect (%) density Example Comparative Example (ppm) One 2 3 4 5 One 2 3 4 5 0 0 0 0 0 0 0 0 0 0 0 10 8.9 6.5 8.1 5.5 7.7 3.2 1.3 3.7 1.4 2.9 30 30.4 25.9 28.3 26.7 27.3 9.6 6.7 11.3 6.9 8.7 50 58.7 49.1 51.9 50.5 55.5 28.7 19.9 27.6 22.3 26.9 70 79.6 70.8 73.1 71.9 78.1 60.1 49.7 62.3 48.1 58.2 90 96.9 95.3 96.7 94.6 98.3 89.9 79.9 85.7 81.3 84.7 100 - - - - - 98.9 95.3 96.4 96.1 98.6 IC50 45.47 49.82 48.11 49.54 46.56 58.13 64.25 58.88 63.68 59.79

As shown in Table 3, it was confirmed that the inhibitory effects of the lipoxygenase activity of Examples 1 to 5, which are the extracts of the crude rice extract obtained by the ultrasonic extraction method, are superior to the inhibitory effects of the lipoxygenase activity of the crude extracts prepared in Comparative Examples 1 to 5 there was.

<Experiment 4> hyaluronic ruro dehydratase activity is inhibited by the extract of herbs jobap effect OK

The effect of inhibiting the activity of hyaruronidase was confirmed by using the dried powder of the crude herbal extract prepared in Examples 1 to 5 and Comparative Examples 1 to 5.

In order to confirm the effect on inhibition of the activity of hyaruronidase, experiments were carried out as follows.

Using the Morgan-Elson method, the final enzyme activity of hyaluronidase was 400 NF unit / ㎖, the final concentration of HA was 0.4 ㎎ / ㎖, and the compound 48/80 buffer solution (0.1 ㎎ / ㎖) Hyaluronidase activity was measured by the inhibition of the activation step of Hyaluronidase. The sample was dissolved in buffer to prepare a sample solution. As a control, a buffer was used instead of the sample solution. For each blank, a buffer was used instead of the enzyme solution, and a green tea extract was used as a control.

The inhibitory effect of the hyparrhoidase activity was determined using the following equation 4, and the results are shown in Table 4.

<Formula 4>

Inhibitory effect of herbal extracts on the activity of hyaruronidase Inhibitory effect of hyaluronidase activity (%) density Example Comparative Example (%) One 2 3 4 5 One 2 3 4 5 0 0 0 0 0 0 0 0 0 0 0 0.5 19.7 10.5 16.7 10.9 16.8 8.8 7.6 9.6 7.1 8.2 One 39.2 23.9 36.3 21.6 35.4 22.7 18.6 19.9 20.8 23.6 3 75.3 46.7 75.2 48.1 77.7 45.2 40.7 39.5 44.2 44.2 5 96.8 94.9 98.1 96.2 98.2 88.6 85.2 87.2 84.1 89.7 IC50 2.10 2.72 2.15 2.70 2.13 3.18 3.34 3.26 3.29 3.16

As shown in Table 4, the effects of inhibiting the hyparrhoidase activity of Examples 1 to 5, which are the extracts of crude aspartame extracted by the ultrasonic extraction method, are superior to the inhibitory activity of the hyarrhonidase activity of the crude extract of Comparative Examples 1 to 5 .

< Experimental Example  5> Sweet pepper  The immune cells of the extract Degranulation  Inhibitory effect

The deagglomeration effects of the immune cells were measured on the dry powder extracts prepared in Examples 1 to 7.

<Experimental Method>

The effect of the present invention on the Rat mast cell line of the RBL-2H3 cell line inhibited degranulation by antigen. The RBL-2H3 cell line was cultured in Dulbeccos' modified Eagle's medium (Dulbecco's modified Eagle's medium, below) supplemented with Dulbecco's containing 1% fetal bovine serum and L-glutamine DMEM) at 37 ° C in a 5% CO ₂ incubator. Suspension cells were suspended in Trypsin-EDTA solution and separated and recovered.

(DNP-HSA) -specific IgE) and then treated with 50 ng / mL of antigen (DNP-HSA) in the activated cells Each of the extracts was treated with 5, 25, and 125 μg / ml and cultured for 60 minutes and 120 minutes. The amount of [beta] -hexosaminidase secreted into the medium was then measured using an ELISA reader. The inhibition of β-hexosaminidase secretion levels means to prevent degranulation of immune cells.

Inhibition of deaggregation by the extract Degranulation (Inhibition rate (%)) 5 μg / ml 25 μg / ml 125 μg / ml Example One 7.86 ± 1.52 18.12 + - 0.47 31.95 ± 2.77 2 3.57 ± 2.09 10.14 + - 2.33 14.88 ± 1.54 3 3.01 + 0.78 10.34 + - 1.86 25.41 + - 0.62 4 5.44 ± 1.63 12.69 + - 0.56 16.23 + - 0.13 5 6.37 + - 0.38 15.88 ± 1.35 26.57 ± 3.83 Comparative Example One 7.91 + 0.04 11.67 ± 0.63 19.68 ± 0.97 2 -1.66 + 2.14 8.91 ± 0.45 13.23 + - 2.43 3 1.63 + - 0.24 6.81 ± 0.59 14.77 + - 0.66 4 2.33 ± 1.12 8.16 ± 1.37 16.37 ± 1.08 5 4.22 ± 0.09 10.82 ± 2.15 16.98 + - 0.37

As shown in Table 5, it was confirmed that the deaggregation inhibition effects of Examples 1 to 5, which are the extracts of crude aspartame extracted by ultrasonic extraction, are superior to the inhibitory activity of hyparrhoidase activity of the crude extract of Comparative Example 1 to 5 there was.

< Experimental Example 6> Clinical evaluation of irritation mitigation of cosmetic composition containing crude extract

For clinical evaluation of the stimulus relaxation of the cosmetic composition containing the crude extract, a cosmetic composition containing the extract of the crude extract was prepared according to the composition shown in Table 6 below.

Composition of cosmetic composition for clinical evaluation number Raw material Prescription example Comparative Example One Dried powder (Example 1) 3.0 - 2 Stearic acid 2.0 2.0 3 Cetyl alcohol 2.0 2.0 4 Glyceryl monostearate 2.0 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 0.5 6 Sorbitan sesquioleate 0.5 0.5 7 Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.0 1.0 8 Wax 1.0 1.0 9 Liquid paraffin 4.0 4.0 10 Squalane 4.0 4.0 11 Caprylic / capric triglyceride 4.0 4.0 12 Carboxyvinyl polymer 0.3 0.3 13 Butylene glycol 5.0 5.0 14 glycerin 3.0 3.0 15 Triethanolamine 0.5 0.5 16 Purified water Balance Balance 17 water - 3.0

To prepare the cosmetic composition of Experimental Example 6, the ingredients 12, 13, 14 and 16 shown in Table 6 were heated to 80 to 85 ° C while mixing and stirring. , 5, 6, 7, 8, 9, 10, and 11 are melted by heating at 80 to 85 ° C, and then the mixture is stirred while being charged into the manufacturing part and emulsified. After the emulsification is completed, the mixture is cooled to 35 ° C with stirring using an agitator, and the mixture is cooled to 25 ° C by 1 time and aged.

In order to prepare the cosmetic composition of the comparative example, the ingredients 12, 13, 14, and 16 shown in Table 6 were heated to 80 to 85 ° C with mixing and stirring, , 6, 7, 8, 9, 10, and 11 are melted by heating to 80 to 85 ° C, and then the mixture is stirred while being charged into the manufacturing part. After the emulsification is completed, the mixture is cooled to 35 ° C with stirring using an agitator, and 17 times is added thereto, followed by cooling to 25 ° C and aging.

The stimulation relaxation effect of the cosmetics prepared above was confirmed through simple clinical evaluation.

Experimental Example 6 In order to evaluate the effect of improving the skin irritation of cosmetics, the following experiment was conducted on 10 volunteers in their 20s and 30s. In order to make the applicant's skin irritation measurement condition the same, the experimental area was kept clean and dry, and the skin was stabilized at a place where constant temperature and humidity (20 ° C, relative humidity 40-60%) was maintained for at least 30 minutes . Skin irritation was evaluated by evaluating the degree of skin irritation using a disposable razor after depilating the lower part of the nose, applying the Comparative Example 1 to the right side of Experimental Example 6 on the left side, . The stimulus measurement was carried out through the self-questionnaire evaluation based on the contents shown in Tables 7 and 8 below, and the difference of the skin irritation was compared with the experiment example 6 and the comparative example.

Skin irritation evaluation standard 0 One 2 3 No stimulation Has a little usually With stimulation

Skin irritation evaluation items and questionnaire Erythema
(Erythema) edema
(Edema) itch
(Itching) Tingling
(Prickling)

The results of the survey are shown in Table 9. Classification Erythema
(Erythema) edema
(Edema) itch
(Itching) Tingling
(Prickling) Prescription example 0 0 0.6 0.4 Comparative Example 0 0 1.9 1.7

As shown in Table 9, it was confirmed that the cosmetic preparation of Experimental Example 6 containing the crude extract (Example 1) extracted by the ultrasonic extraction method was superior to the cosmetic preparation prepared by correcting water (Comparative Example) instead of the extract .

< Formulation Example  1> Sweet pepper  Preparation of lotion containing extract

Table 10 shows formulation examples of lotion (skin lotion) among the cosmetic compositions containing the dry powder of the crude extract of Example 1.

Composition of Lotion Containing Extracts of Herb Extracts number Raw material Content (% by weight) One  Dried powder (Example 1) 1.0 2  glycerin 3.0 3  Butylene glycol 2.0 4  Propylene glycol 2.0 5  Polyoxyethylene hardened castor oil 1.0 6  ethanol 10.0 7  Triethanolamine 0.1 8  antiseptic a very small amount 9  Pigment a very small amount 10  Spices a very small amount 11  Purified water Balance

In order to prepare the lotion, the emulsion was added in the order of No. 2, 3, 4, and 8 described in Table 10, followed by dissolving by stirring. The emulsion was heated by heating at about 60 ° C for 5 times, After dissolution, it was added at 11 times. Finally, 1,6,7 and 9 were added and agitated after sufficient stirring.

< Formulation Example  2> Sweet pepper  Manufacture of nutrition lotions containing extracts

Table 1 shows formulation examples of nutritional lotion among the cosmetics containing the dry powder of the crude extract of Example 1.

Composition of nutritional lotion containing crude extract number Raw material Content (% by weight) One  Dried powder (Example 1) 2.0 2  Wax 1.0 3  Polysorbate 60 1.5 4  Sorbitan sesquioleate 0.5 5  Liquid paraffin 10.0 6  Sorbitan stearate 1.0 7  Pro-type glycerin monostearate 0.5 8  Stearic acid 1.5 9 Glyceryl stearate / FG-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16  Purified water Balance

To prepare the nutrition lotion, the ingredients 10, 11, 13, and 16 described in Table 11 were heated to 80 to 85 ° C while mixing and stirring, , 7, 8, 9 and 12 were melted by heating at 80 to 85 ° C and emulsified. After the emulsification was completed, the mixture was cooled to 50 ° C. with stirring using an agitator, and then the mixture was cooled to 45 ° C. and then added to the mixture. The mixture was cooled to 25 ° C. and then aged.

< Formulation Example  3> Sweet pepper  Preparation of nutritional cream containing extract

Table 12 shows formulation examples of the nutritional cream among cosmetics containing the dry powder of the crude extract of Example 1.

Composition of nourishing cream containing extract number Raw material Content (% by weight) One Dried powder (Example 1) 3.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / capric triglyceride 4.0 12 Carboxyvinyl polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Balance

In order to prepare the nutritional cream, the ingredients 12, 13, 14, and 16 described in Table 12 were heated to 80 to 85 ° C while mixing and stirring, 7, 8, 9, 10 and 11 were melted by heating to 80 to 85 ° C, and then 15 parts of the mixture were stirred and introduced into the production part and emulsified. After completion of the emulsification, the mixture was cooled to 35 DEG C with stirring using an agitator, and the mixture was cooled to 25 DEG C by one time and aged.

< Formulation Example  4> Sweet pepper  Preparation of Essences Containing Extracts

Table 13 shows formulation examples of essences in cosmetics containing the dried powder of the crude extract of Example 1.

Composition of essence containing crude extract number        Raw material Content (% by weight) One  Dried powder (Example 1) 3.0 2 Sitosterol 1.7 3  Polyglyceryl 2-oleate 1.5 4  Ceramide 0.7 5 Steareth-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11  Xanthan gum 0.2 12  antiseptic a very small amount 13  Spices a very small amount 14  Purified water Balance

In order to prepare the essence, homogenization at a constant temperature of 2, 3, 4, 5 and 6 in Table 13 is referred to as nonionic amphipathic lipid. The non-ionic amphipathic lipid was mixed with 1, 7, 8 and 14, homogenized at a constant temperature, passed through a microfluidizer and then gradually added at a constant temperature to homogenize the microfluidizer. And then 10, 11, 12 and 13 were added thereto, dispersed, stabilized and aged.

The present invention is only an example of a cosmetic composition for improving antioxidant, antiinflammation and atopic skin comprising an extract of Asahibusan ash extract by the ultrasonic extraction method, a method for producing the extract, and an extract of Asahi extract as an active ingredient. The present invention can be embodied in various other forms without departing from the scope of the present invention, and it is to be understood that the present invention may be embodied in many other specific forms without departing from the spirit or essential characteristics thereof. And the present invention is only defined by the scope of the claims.

Claims (8)

A step (T1) of mixing the crude oil and one or more solvents selected from the group consisting of water, ethanol or 1,3-butylene glycol, and then immersing the mixture in a solvent to obtain a crude oil deposit;
(S2) a step of injecting the above-mentioned cooked herb seeds into an ultrasonic wave extractor and irradiating the ultrasonic wave with ultrasonic waves to obtain a crude extract of the present invention (S2).
(S1) a step of mixing the crude herb with water, ethanol, and a mixed solvent of 1,3-butylene glycol and then immersing the mixture to obtain a crude egg deposit;
(S2) a step of injecting the above-mentioned cooked herb seeds into an ultrasonic wave extractor and irradiating the ultrasonic wave with ultrasonic waves to obtain a crude extract of the present invention (S2).
3. The method according to claim 1 or 2,
The method of producing the crude extract of the present invention by the ultrasonic extraction method, wherein the crude oil and the solvent are mixed in the step (T1) or the step (S2) and then the mixture is immersed at 4 to 50 ° C for 1 to 24 hours .
3. The method according to claim 1 or 2,
In step (S2), the crude extract is extracted and irradiated with ultrasonic waves at 4 to 50 ° C, 20 to 60 KHz and 100 to 700 Watt for 20 to 20 hours, and then extracted with the ultrasonic extraction method. Way.
3. The method according to claim 1 or 2,
Further comprising a step (S3) of cooling, filtering and concentrating the crude extract of the crude as obtained in the step (S2).
The crude extract of the present invention produced by the method of claim 1 or 2,
Antioxidant, antiinflammatory, and atopic skin, and has an effect of promoting cell proliferation.
The crude extract of the present invention prepared by the method of claim 4,
Antioxidant, antiinflammatory, and atopic skin, and has an effect of promoting cell proliferation.
The crude extract of the present invention prepared by the method of claim 5,
Antioxidant, antiinflammatory, and atopic skin, and has an effect of promoting cell proliferation.

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