KR101690145B1 - A cosmetic composition comprising liposome of the cold leaf extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a gold green tea extract liposome as an active ingredient, and a cosmetic composition comprising the gold green tea extract of the present invention, preferably a gold green tea extract liposome, as an active ingredient, Which is expected to be useful as a cosmetic for enhancing skin elasticity or preventing aging.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition containing a gold green tea extract liposome as an active ingredient,

The present invention relates to a cosmetic composition containing a gold tea extract, and more particularly to a cosmetic composition comprising a gold tea extract, preferably a gold tea extract liposome as an active ingredient.

Skin is the primary barrier of the human body. It protects the internal organs of the body from external stimuli such as changes in temperature and humidity, ultraviolet rays, and pollutants, and plays an important role in the maintenance of body homeostasis such as body temperature control. This important skin also becomes aged gradually as the age of the human body grows, and as a result, skin elasticity loss, keratinization, wrinkle formation, skin atrophy and the like appear. This aging of the skin not only causes wrinkles on the skin surface but also changes the entire line of the face.

The appearance of wrinkles on the skin is a phenomenon accompanied by aging, which shows various structural changes due to the deterioration of vital function. That is, it is divided into intrinsic aging and aging caused by aging, and photoaging due to the addition of external factors, especially ultraviolet damage. There are many kinds of outsiders, but they are most affected by ultraviolet rays in sunlight. In the endogenous aging process, characteristic clinical skin findings include fine wrinkles, atrophy of the dermis, and reduction of the subcutaneous fat layer. In the photoaging process, rough and deep wrinkles appear and abnormal elastic fibrous material accumulates and the skin becomes thick and loosens like leather .

Cosmetics has been used as a means to protect oneself or to express religious necessity or status and class. However, as the development of industry has expanded the consumer class and the psychological desire to be more beautiful, it has become a cultural product Recently, it has been changed to cosmetics that can maintain beauty and beauty together. As such, the cosmetics industry is growing rapidly and systematically and scientifically, and the level of consumers' cosmetics is also rising. However, due to environmental pollution caused by the development of industrialization and science, modern people are focusing more attention on safety and health. Naturalism and environmental friendliness, as seen in the trend of modern cosmetics, It can be said that desire is showing well. As the trend of this age has influenced the cosmetics industry, consumers are using various cosmetics to protect their skin from damage due to various harmful factors, to maintain health and beauty. Among them, cosmetics with natural concept Not only has it received much attention, but the related consumer market continues to grow steadily every year.

In recent years, interest and needs of consumers have been focused on functional cosmetics that can maintain health and beauty through a stronger improvement effect from simple beauty through improved quality of life such as well-being and LOHAS. In addition, as the standard of living improves, economics, convenience, and practicality have become important factors in consumption patterns. In the selection of cosmetics, it is better to use unconditional famous brands, expensive cosmetics, And the trend toward a product that can realize the functional effect is changing.

However, as the size and influence of the entire cosmetics industry have increased, the leading technology development in the related field has become an essential issue. However, the cosmetics industry has only achieved world-class growth for 10 years, Has not reached the development level of developed countries, and the level of development of new materials has been lagging behind. In addition, the domestic consumer market for imported cosmetics has been inundated, and products with unproven product efficacy and skin safety have been imported indiscriminately. The reliability of cosmetics has been decreasing and the competitiveness of cosmetics industry has been losing its competitiveness due to increasing dependency on imports of major raw materials for cosmetics and the lack of base technology.

In addition, limitations of efficacy on commonly used materials are continuously being clarified. In view of the technical aspects used in products, various mechanisms for expressing the respective functional effects can exhibit optimal functions through interaction. However, Most of the products are given functionalities to correspond to the localized parts, so they often fail to show their efficacy. Therefore, it is urgent to investigate the effect of these products because consumers are often skeptical about the efficacy of the products. In addition, in the utilization of originally developed materials, there are restrictions on the use of exclusive rights such as patent and monopoly by each individual.

Recently, it is very timely to learn about green tea at a time when the trend of green tea is spreading to the best ever, and green tea is naturally rooted throughout our life, . Green tea is getting attention in a wide variety of fields such as beverages, food ingredients, and daily necessities because green tea is so useful to human beings. Among them, cosmetics using organic green tea, Jeju green tea, Boseong green tea, etc. have been launched (Innisfree Green tea seed line etc.) among domestic products, and cosmetics using green tea as a concept outside of the country are spread all over the world (Elizabeth Arden Green Tea, etc.). Among the cosmetic compositions using green tea, cosmetics containing EGCG derivatives (Preparation Method thereof and a cosmetic composition thereof) are used. When applied to skin, they inhibit oxidation by active oxygen in vivo and inhibit the production of peroxides, And has an effect of promoting collagen biosynthesis. It has no stimulation to the skin and has an antioxidant function, an increase in the elasticity of the skin, and a prevention and improvement of skin aging.

Meanwhile, gold tea, which is known to be the most famous among green tea of Korea recently, is added to gold, which is a gold tea with gold added to it. The gold in the golden tea is stable in Dongbokbo feeling, nurturing action, Are known to have efficacy. In addition, recent studies on gold in US and European countries have revealed various effects such as strengthening immunity, promoting blood circulation, activating skin, relieving pain in arthritis, and promoting brain activity. In Korea, it is said that "gold (gold) is a mixture of ingredients, flavor, poison, jin, a soul, a white heart, It is written that the gold medal of the five rods and the actual symptoms of the five rods are healed and the children's game is healed. "It is said that gold calms the mind, stabilizes the mind, As shown in Fig.

However, there are not many known techniques for applying gold skin, and they are used only as a concept that is visually seen even in cosmetics. Colloidal Gold is a solution of this disadvantage. It is a golden car that was developed using this colloidal gold solution.

The gold tea using Colloidal Gold is the world's first and the only one that combines the efficacy of gold and tea by adding gold mineral to the tea leaves that are grown by giving Colloidal Gold to 7,000 pyeong and 110 million won gold It is a precious difference. In addition, the reason for realizing a perfect environment friendly farming method that exceeds the international organic certification standards such as eliminating the use of all pesticides, herbicides, chemical fertilizers, and manure, sticking steaming, and catching insects by hand, 4.

Therefore, the present inventors have been continuing research to develop a cosmetic material having improved skin elasticity and wrinkle-improving effect by using a material which is one step closer to consumers' desire to use high-performance cosmetics and has assured certain efficacy and safety. The present invention has been completed by preparing a cosmetic containing an extract of green tea as an active ingredient.

The present invention provides a cosmetic composition comprising a green tea extract liposome as an active ingredient.

In addition, the present invention provides a method for producing the gold green tea extract liposome.

In order to solve the above technical problems, the present invention provides a cosmetic composition comprising a gold green tea extract liposome as an active ingredient.

According to another aspect of the present invention, there is provided a method of preparing the gold green tea extract liposome.

The cosmetic composition comprising the gold tea extract of the present invention, preferably a gold green tea extract liposome as an active ingredient, is expected to be useful as a cosmetic for enhancing skin elasticity or preventing aging, because it is highly safe as compared with conventional chemical cosmetic compositions.

BRIEF DESCRIPTION OF THE DRAWINGS The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate exemplary embodiments of the invention and, together with the description of the invention, It should not be construed as limited.
Fig. 1 is a standard calibration curve for measuring the content of gold in the green tea extract.
2 is a graph showing the content of catechins in gold and green tea measured.
3 is a graph showing the content of catechins in gold green tea extract.
4 is a graph showing the content of catechins in the liposome of gold green tea extract measured.
5 is a graph showing the viscosity of gold green tea extract according to temperature measured over time.
6 is a graph showing the buoyancy of a cream containing 1% by weight of a green tea extract.
7 is a graph showing the buoyancy of a cream containing 3% by weight of a green tea extract.
8 is a graph showing the degree of skin elasticity improvement (R7) in the case of using a cream containing a green tea extract.
9 is a graph showing the degree of skin elasticity improvement (R7) when a bibby cream containing a green tea extract is used.

The present invention relates to a cosmetic composition comprising a gold green tea extract liposome as an active ingredient.

Preferably, the present invention provides a cosmetic composition for improving skin elasticity containing a gold green tea extract liposome.

The green tea extract contained as an active ingredient in the cosmetic composition of the present invention can be extracted according to a conventional extraction method. For example, a green tea leaf can be pulverized, extracted with an ethanol solution, and then filtered and concentrated. have.

As the extraction solution, a 20 to 40% ethanol solution, preferably a 30% ethanol solution is used, and the extraction is carried out at 60 to 100 ° C, preferably 80 ° C for 4 to 6 hours, preferably 5 hours . Then, the extracted gold green tea extract is filtered with a 0.2 to 5 탆 mesh, and then concentrated at 50 to 80 캜, preferably 60 캜, to prepare a gold tea extract. The gold tea extract prepared is in powder form.

The gold tea which is an effective ingredient of the cosmetic composition of the present invention is a product which gives a gold solution (colloidal gold) to a tea plant. Specifically, it is used for a gold solution cycle, tea leaf picking, sorting and sorting, scouring, drying, storage, sorting, , And the cultivation conditions of the gold tea of the present invention are the same as the general cultivation conditions of the tea tree

The amount of gold solution during the cultivation of gold tea is somewhat different according to the weather conditions, but 200L of colloidal gold solution (Colloidal Gold) is added 100 times in the vicinity of the tea roots at the end of April, 10-15 days before the young leaves Of water for the first time. After that, 100L of water is added once a month at intervals of one month until the end of July. At this time, the size of the gold solution is 3 nm or less, the concentration of the gold solution is about 50 ppm, and the amount of the gold solution (amount / area of the solution) is 500 L / 20,560 m 2.

Generally, green tea which does not give gold solution and quasi-gold tea and gold solution does not yield yield, green tea There is no significant difference in the size, etc.

In the present invention, the gold tea extract can be made into liposomes, which are stable in an aqueous solution state and in a state of excellent skin permeability, and can be used.

The liposomalization of the green tea extract can be prepared by the following method.

(1) The lipid-stabilizing portion and the hydrophilic activating material are melted by dissolving the lipid thickening portion and the lipophilic active material portion at 45 ° C to 50 ° C in the range of 70 ° C to 80 ° C.

(2) The lipophilic active material is dissolved in the lipid concentrate, and the resulting mixture is added to the lipid stabilizer. The mixture is stirred at an agitation speed of 1,000 to 2,000 rpm and uniformly mixed.

(3) A portion of the hydrophilic active material is put into the mixture and stirred at a rotation speed of 1,000 to 2,000 rpm to uniformly mix.

(4) Liposomes are formed by emulsification three to four times using a high pressure emulsifier.

The gold green tea extract liposome is composed of a structure and composition similar to skin structure using materials constituting phospholipid, which is an important point for delivering the skin to the skin, stabilizing and transferring the pharmacological material (Ceramide / Cholesterol / Phospholipid complex ), Ceramide and Cholesterol give skin moisturizing effect and Vitamin-E Acetate, an antioxidant, helps to skin antioxidant.

The gold tea extract of the present invention may be contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.

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In addition, the cosmetic composition containing the gold tea extract of the present invention may contain, in addition to the above extracts, conventional auxiliary agents and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, can do.

The cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant- But are not limited to, cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. More particularly, the present invention relates to a cosmetic lotion composition comprising a lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a baboon cream, a seed cream, a hair tonic, a shampoo, a rinse, a cleansing cream, a cleansing foam, a cleansing water, May be prepared in a formulation.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butylglycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition comprising the gold tea extract of the present invention, preferably a gold green tea extract liposome as an active ingredient, is expected to be useful as a cosmetic for enhancing skin elasticity or preventing aging, because it is highly safe as compared with conventional chemical cosmetic compositions.

Further, the present invention provides a make-up method for improving skin elasticity, which comprises applying a cosmetic composition containing gold tea extract to human skin.

The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having excellent skin protection effect according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied depending on the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

When the cosmetic composition comprising the gold green tea extract of the present invention is applied to human skin, it is very useful for preventing aging of the skin due to its penetration into the skin.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1 Preparation of Gold Green Tea Extract

The harvested gold tea was pulverized and then extracted with 30% ethanol at 80 ° C for 5 hours.

Then, the mixture was filtered through a 5-mesh screen, and then concentrated at 60 ° C to prepare a gold tea extract powder.

Example 2 Preparation of Gold Green Tea Extract Liposome

According to general liposomal technology, gold green tea extract liposomes having the compositions shown in Table 1 below were prepared.

The phospholipids used herein are Lecinol S-10 (Nikko Chemical), Lipoid S 75-3 (Lipoid), Lipoid S100 (Lipoid), and lipophilic antioxidants Is Vitamin E Acetate (BASF, Germany).

Test Example 1 Measurement of Gold Content of Gold Green Tea Extract

The gold content of the green tea extract prepared in Example 1 was measured by the following method. The standard calibration curve is shown in Fig.

(1) Operating conditions

Ingredients: Green tea, green tea extract

Analytical Instruments: GENESIS ICP-OES

Test material: Au

Test environment Temperature and Humidity: 23 ℃, 37% R, H

(2) Test method

① Take about 0.2 g of this product precisely.

② Add 3 ml of sulfuric acid, 3 ml of nitric acid, and 1 ml of hydrofluoric acid. Heat the mixture at 180 ° C for 20 minutes using a microwave dissolver.

3. Add distilled water of ultrapure water to the dissolved solution to make exactly 50 ml, and use this solution as the sample solution.

(3) Standard solution: Au - 0.01 ppm, 0.1 ppm, 1 ppm

(4) Content formula

The content was calculated according to the following formula (1).

In this formula,

A is the gold calibration value (ppm) obtained from the calibration curve,

D represents the dilution volume (50 ml)

S represents the sample weight (g).

The gold content of the gold and green tea extract powder analyzed according to the above test method is shown in Tables 2 and 3 below.

Sample intake (g) Au measurement (ppm) Gold content in specimen (ppm) 0.2017 0.032 7.93

Sample intake (g) Au measurement (ppm) Gold content in specimen (ppm) 0.2032 0.028 6.89

Test Example 2 Measurement of Catechin Content of Gold Green Tea Extract

The catechin content of the green tea extract prepared in Example 1 was measured by the following method.

(1) Equipment

Agilent 1200 Series

Quaternary Pump

Auto Sampler

Diode Array Detector

Agilent TC-C18 (2), 5 [mu] m, 4.6 x 250 mm

(2) Reagent

(-) - Catechin reference material

- Manufacturer: Sigma-Aldrich

- Purity: 100.0%

- Manufacturing number: 110M1438V

(-) - Epigallocatechin gallate reference material

- Manufacturer: Sigma-Aldrich

- Purity: 95.0%

- Manufacturing number: 071M1809V

(-) - Epicatechin reference material

- Manufacturer: Sigma-Aldrich

- Purity: 99.0%

- Manufacturing number: SLBB3492V

(-) - Epicatechin gallate reference material

- Manufacturer: Sigma-Aldrich

- Purity: 99.0%

- Manufacturing number: SLBC1851V

- Acetonitrile (HPLC Grade)

- Methanol (HPLC Grade)

- Tetrahydrofuran (HPLC Garde)

- Formic acid

(3) Experimental conditions

Was performed according to experimental conditions as shown in Table 4 below.

Flow rate 0.6 mL / min Detector 280 nm Dose 10 uL Mobile phase A) 4.5% Formic acid
B) 90% ACN + 10% A = 8: 2 Column temperature 25 ℃ Analysis time 30 minutes

(4) Standard solution preparation

- (-) About 3 mg of Catechin standard, about 2 mg of Epicatechin standard and about 5 mg of Epicatechin gallate standard are added to a 10 mL volumetric flask, dissolved in methanol, To make a standard solution.

- (-) - Epigallocatechin gallate Precisely weigh approximately 12 mg of the standard material and dissolve in a 100-mL volumetric flask with methanol. Fill the scale well to make the standard solution.

(5) Preparation of test liquid

- About 0.3 ~ 1.0 g of this sample is precisely weighed, placed in a 50 mL volumetric flask, dispersed with 5 mL of tetrahydrofuran, and methanol is added to fill the scale accurately.

- Dispense the supernatant with ultrasonic waves and filter it to prepare the sample solution.

(6) Calculation

The catechin content can be calculated according to the following equation (2).

(7) Measurement data

The measurement data are shown in Table 5 below, and the contents of Caechin in the gold tea, gold green tea extract and gold green tea extract liposome are shown in Table 6, FIG. 2, Table 7, FIG. 3, Respectively.

Measurement Item unit Green tea Green tea
extract Green tea
extract Liposome Amount of sample g 0.5421 0.3836 0.9213 (-) - Catechin peak area of the test solution MAU * s - - - (-) - Epigallocatechin gallate Peak area of the sample solution 4863.60 14252.70 - (-) - Peak area of Epicatechin test solution - - - (-) - Epicatechin gallate Peak area of the sample solution 2404.80 7008.99 - (-) - Amount of Catechin standard material mg 3.6 (-) - Amount of Epigallocatechin gallate standard material 12.7 (-) - Amount of Epicatechin standard material 2.2 (-) - Amount of Epicatechin gallate standard material 5.8 (-) - Peak area of Catechin standard solution MAU * s 2817.86 (-) - Peak area of Epigallocatechin gallate standard solution 1680.29 (-) - Peak area of Epicatechin standard solution 2086.83 (-) - Peak area of Epicatechin gallate standard solution 13694.0

Test Items unit Test result Test Methods (-) - Catechin % Not detected (detection limit 0.001) HPLC (-) - Epigallocatechin gallate 3.22 (-) - Epicatechin No detection (detection limit 0.005) (-) - Epicatechin gallate 0.93

As shown in Table 6, the content of Caechin in the green tea was (-) - Catechin (not detected), (-) - Epigallocatechin gallate (3.22%), Epicatechin (not detected) (0.93%), and (-) - Epigallocatechin gallate was the highest.

Test Items unit Test result Test Methods (-) - Catechin % Not detected (detection limit 0.001) HPLC (-) - Epigallocatechin gallate 13.34 (-) - Epicatechin No detection (detection limit 0.005) (-) - Epicatechin gallate 3.83

As shown in Table 7, the content of Caechin in the green tea extract powder was (-) - Catechin (not detected), (-) - Epigallocatechin gallate (13.34%), Epicatechin (not detected) gallate (3.83%), and (-) - Epigallocatechin gallate was the highest.

Test Items unit Test result Test Methods (-) - Catechin % Not detected (detection limit 0.001) HPLC (-) - Epigallocatechin gallate Not detected (detection limit 0.01) (-) - Epicatechin No detection (detection limit 0.005) (-) - Epicatechin gallate Not detected (detection limit 0.05)

As shown in Table 8, the Caechin content of the gold green tea extract liposome was (-) - Catechin (not detected), (-) - Epigallocatechin gallate (not detected), (-) - Epicatechin gallate (not detected).

From the above results, (-) - Epigallocatechin gallate and (-) - Epicatechin gallate were detected in gold and green tea extract powder, but not in gold green tea extract liposome. (-) - Epigallocatechin gallate and (-) - Epicatechin gallate were unstable due to liposomal degradation.

≪ Formulation Example 1 > Preparation of Lotion Formulation Containing Gold Green Tea Extract

Lotion formulations containing a green tea extract were prepared according to the composition ratios shown in Table 9 below.

≪ Formulation Example 2 > Preparation of cream containing gold tea extract

A cream formulation containing a green tea extract was prepared according to the composition ratios shown in Table 10 below.

The emulsifier, oil, antioxidant and preservative are dissolved in the oily dissolution at 80 ~ 82 ℃, and some additives and solvent are added to the manufacturing part heated to 78 ~ 80 ℃ and emulsified with HOMO MIXER to 40 ℃ Cooling, and the other additives were added, and the mixture was stirred and cooled.

≪ Formulation Example 3 > Preparation of Bibi Cream Formulation Containing Gold Green Tea Extract

According to the composition ratios shown in the following Table 11, a bibby cream formulation containing a green tea extract was prepared.

≪ Test Example 3 > Measurement of pH stability

The pH stability of the cream containing the green tea extract of the present invention was measured. The pH of cosmetics is matched to the acidity and neutrality of the pH range of the surface of the skin. When the skin becomes alkaline, it is recommended to use neutral or weakly acidic cosmetics because skin is less resistant and inflammation is likely to occur by the propagation of bacteria. Also, the change in pH should be stable and not large.

The pH measurement results are shown in Table 12 below.

In Table 12, A represents a control group, B represents a cream containing 0.5% of a green tea extract, C represents a cream containing 1.00% of a green tea extract, and D represents 3.00% cream of a green tea extract.

As shown in Table 12, it was confirmed that the green tea extract-added cream maintained a stable pH value without significant change in the number of days for 58 days.

<Test Example 4> Viscosity Measurement

The viscosity of the cream containing the gold tea extract of the present invention was measured at 4 캜, 25 캜, 44 캜 and 50 캜, respectively, and the results are shown in Fig. In FIG. 5, A: control group, B: cream containing 0.5% of gold green tea extract, C: cream containing 1.00% of gold green tea extract, and 3.00% cream of D:

As shown in FIG. 5, the viscosity after one day and the viscosity after 58 days after the cream containing the green tea extract were prepared. Showed little change for 58 days and was stable.

&Lt; Test Example 5 >

Since cosmetics contain a large amount of water, contamination by bacteria or fungi tends to occur. Products contaminated with microorganisms may not only cause quality degradation such as odor, color change, viscosity change, texture change, fungus generation, but also cause harmful diseases such as serious eye diseases such as corneal ulcers. For this reason, in order to maintain the quality of products in cosmetics for a long time, preservatives that prevent microbial decay are essential and the distribution period is relatively long.

Therefore, the antifogging property of the cream containing the gold green tea extract of the present invention was observed.

6 is a graph showing the buoyancy of a cream containing 1% of a green tea extract and FIG. 7 is a graph showing a buoyancy of a cream containing 3% of a green tea extract.

As shown in FIGS. 6 and 7, the cream containing the green tea extract was reduced to less than 0.1% after 7 days of inoculation, and no further increase was observed on days 14, 21, and 28. In addition, mold and yeast were reduced to less than 0.1% of the inoculum after 7 days, and there was no further increase on days 14, 21, and 28. Therefore, it was found that the number of inoculated bacteria decreased more than 99.9% after 7 days.

<Test Example 6> Skin elasticity improvement effect test

This test is a human-applied test that evaluates the elasticity-improving efficacy of a subject using a test equipment Cutometer® (Courage & Khazaka, Germany) for a period of 4 weeks. The measurement index is a change in elasticity Respectively. The skin elasticity improvement effect of the cream containing the green tea-containing composition prepared in Formulation Example 2 was tested.

Test equipment Cutometer is a device that can measure skin's distensibility and elasticity by using viscoelastic property of skin by using inhaled steel.

1. Selection of subjects

We selected 23 subjects who met the following criteria and did not fall under the exclusion criteria.

1) A person who is 30 years old or older and under 50 years old and has been judged to have a skin condition suitable for the test by a dermatologist

2) A person who has received a sufficient explanation of the examination (examination process, possible side effects, etc.) and decided to participate in the examination with free will

3) The person who can perform smooth follow-up observation during the test period

2. Application method

The subjects were self-administered twice a day, morning and evening, for a total of four weeks.

3. Evaluation Method

a. Instrument evaluation with Cutometer®

b. Safety evaluation by dermatologist

4. Test sequence

1) First visit date

- The use of basic products was prohibited 12 hours before the visit.

- The subjects listened to the test method, schedule, risk and possible adverse reaction, wrote basic information and signed the agreement.

- After cleansing using a standard cleanser provided by the tester, it was patted with a paper towel to remove moisture, and then stabilized under constant temperature and humidity conditions (20 to 24 ° C, 40 to 60% RH) for 30 minutes.

- The measurement site was divided as follows so that measurement can be made at the same site every evaluation.

- 1/2 point of hypothetical line connecting the lip commissure and ipsilateral tragus

- Cutometer Parameter setting: On-time 2 sec / Off-time 2 sec / Pressure 450 mbar / Repetition 3 times

- The product was distributed after training the subject notes and product usage.

- During the test period, compliance with product use was checked daily by telephone.

2) Second visit (2nd week)

- Device evaluation was conducted at the same site in the same way as the first visit.

- The dermatologist evaluated the adverse reaction.

3) Third visit (4th week)

- Device evaluation was conducted at the same site in the same way as the first visit.

- The dermatologist evaluated the adverse reaction.

- After using the questionnaire.

5. Evaluation and analysis methods

(1) Evaluation of elasticity improvement effect using cutometer

: The statistical significance between the measurements of skin elasticity measured before, after 2 weeks, and 4 weeks after use of the measurement site was analyzed and verified using Wilcoxon signed rank test.

: The degree of improvement of the elasticity effect was derived as a percentage value according to the following equation (3).

(2) Safety evaluation by a dermatologist

: Evaluated the occurrence of side effects (erythema, edema, scurvy, itching, sore throat, burning sensation, stiffness, tingling, and other abnormalities)

6. Results

The test results are shown in Table 13 (skin elasticity measurement value (R7) - right side), Table 14 (skin elasticity measurement value (R7) - left side) and Table 15 (skin elasticity measurement value (R7) change). 8 shows the skin elasticity improvement degree (R7).

ID Before using the product After 2 weeks of use After 4 weeks of use One 0.4720 0.4824 0.5246 2 0.3529 0.3710 0.3818 3 0.4606 0.4972 0.5242 4 0.4629 0.4740 0.4831 5 0.4066 0.4590 0.4866 6 0.3575 0.4133 0.4387 7 0.3611 0.3889 0.4128 8 0.3311 0.3879 0.4464 9 0.4030 0.4159 0.4298 10 0.3705 0.3941 0.4571 11 0.3810 0.4059 0.4299 12 0.3061 0.3122 0.3447 13 0.4556 0.4644 0.4908 14 0.3503 0.3750 0.3941 15 0.3599 0.3759 0.4042 16 0.4455 0.4526 0.4591 17 0.3245 0.3309 0.3824 18 0.4033 0.4269 0.4342 19 0.3268 0.3396 0.3827 20 0.3531 0.3661 0.4038 21 0.2796 0.3415 0.3729 22 0.2931 0.3232 0.3500 23 0.3639 0.4051 0.4394

ID Before using the product After 2 weeks of use After 4 weeks of use One 0.4784 0.5128 0.5326 2 0.3558 0.3742 0.3953 3 0.4508 0.4848 0.5032 4 0.4604 0.4776 0.4882 5 0.4044 0.4564 0.4744 6 0.3473 0.4029 0.4367 7 0.3776 0.3988 0.4231 8 0.3230 0.3622 0.4075 9 0.3980 0.4148 0.4253 10 0.3687 0.3826 0.4363 11 0.3948 0.4237 0.4387 12 0.3036 0.3102 0.3315 13 0.4576 0.4680 0.4987 14 0.3581 0.3769 0.4045 15 0.3597 0.3742 0.4216 16 0.4447 0.4488 0.4534 17 0.3258 0.3391 0.3851 18 0.4056 0.4292 0.4349 19 0.3102 0.3333 0.3736 20 0.3491 0.3634 0.3987 21 0.2838 0.3452 0.3793 22 0.3006 0.3367 0.3794 23 0.3750 0.4140 0.4450

Before use  After 2 weeks of use After 4 weeks of use Right measurement 0.3748 0.4001 0.4293 Right Elasticity Improvement Rate (%) - 6.75% 14.53% P-value -  <0.001 * <0.001 * Left Measured Value 0.3753 0.4013 0.4290 Left Elasticity Improvement Rate (%) - 6.91% 14.29% P-value - <0.001 * <0.001 *

* P-value < 0.05

A total of 23 subjects were tested for skin elasticity improvement efficacy of the nutritional cream containing gold green tea of the present invention for a total of 4 weeks. All 23 subjects completed the test without a dropout.

As a result, as shown in Tables 13 and 14, the improvement rate of the elasticity of the right and left test sites was 6.75% and 6.91% after 2 weeks of use, 14.53% and 14.29% after 4 weeks of use, It was confirmed that there was a statistically significant difference from the week after the last use.

In addition, the skin adverse reaction for 4 weeks using cosmetics was evaluated and shown in Table 16 below.

As a result, no specific adverse events were observed in all subjects during the study.

&Lt; Test Example 7 >

The skin elasticity improvement effect test was performed on the bibby cream containing the gold-green tea-containing composition of the present invention prepared in the above-mentioned Formulation Example 3, in the same manner as in Test Example 3 above. However, 22 subjects were used once a day for measurement.

Test results are shown in Table 17 (skin elasticity measurement value (R7) - right side), Table 18 (skin elasticity measurement value (R7) - left side) and Table 19 (skin elasticity measurement value (R7) change). 8 shows the degree of improvement in elasticity (R7). 9 shows the degree of skin elasticity improvement (R7).

ID Before using the product After 2 weeks of use After 4 weeks of use One 0.4844 0.4967 0.5152 2 0.4091 0.4255 0.4751 3 0.4692 0.4826 0.5201 4 0.3539 0.3631 0.3668 5 0.3937 0.3896 0.4513 6 0.4211 0.4566 0.4768 7 0.4668 0.4489 0.4952 8 0.4614 0.4694 0.5062 9 0.323 0.3411 0.3724 10 0.3903 0.4394 0.4092 11 0.3276 0.3591 0.4172 12 0.3139 0.3323 0.3683 13 0.3456 0.347 0.3662 14 0.4059 0.4061 0.4408 15 0.3606 0.4127 0.4139 16 0.4295 0.4401 0.4722 17 0.3548 0.3447 0.3519 18 0.3314 0.3708 0.3976 19 0.3402 0.3793 0.4 20 0.3282 0.3483 0.3892 21 0.3781 0.3792 0.4103 22 0.4245 0.4292 0.4385

ID Before using the product After 2 weeks of use After 4 weeks of use One 0.4872 0.506 0.5147 2 0.4066 0.4229 0.4548 3 0.4654 0.4775 0.5068 4 0.3597 0.362 0.3722 5 0.3887 0.3858 0.4249 6 0.4178 0.4344 0.4735 7 0.4678 0.4551 0.5118 8 0.4665 0.4785 0.514 9 0.3674 0.3452 0.3733 10 0.4164 0.436 0.4012 11 0.3597 0.3806 0.4232 12 0.3302 0.355 0.3907 13 0.3539 0.3462 0.3621 14 0.3655 0.3874 0.4102 15 0.3479 0.4056 0.4115 16 0.4138 0.4269 0.4706 17 0.3546 0.3439 0.3633 18 0.3293 0.3636 0.4135 19 0.3379 0.3531 0.3841 20 0.3242 0.3599 0.4019 21 0.3785 0.3973 0.4113 22 0.4072 0.4298 0.4533

Before use  After 2 weeks of use After 4 weeks of use Right measurement 0.3870 0.4028 0.4297 Right Elasticity Improvement Rate (%) - 4.09% 11.06% P-value -  <0.001 * <0.001 * Left Measured Value 0.3885 0.4024 0.4292 Left Elasticity Improvement Rate (%) - 3.59% 10.49% P-value - <0.002 * <0.001 *

* P-value < 0.05

As shown in Tables 17 and 18, the improvement rate of the elasticity of the test site was 4.09% and 3.59% after 2 weeks of use on the right cheek and left cheek, respectively, and 11.06% and 10.49% after 4 weeks of use, respectively. It was confirmed that there was a statistically significant difference from the week after the use.

In addition, the skin adverse reaction for 4 weeks using cosmetics was evaluated and shown in Table 20 below.

As a result, no specific adverse events were observed in all subjects during the study.

In conclusion, cosmetics using the cosmetic composition containing the green tea of the present invention exhibited statistically significant improvement in elasticity from the second use of the present invention. In addition, no abnormal adverse reaction was observed during use, and it was judged as a safe product.

The cosmetic composition comprising the gold tea extract of the present invention, preferably a gold green tea extract liposome as an active ingredient, is expected to be useful as a cosmetic for enhancing skin elasticity or preventing aging, because it is highly safe as compared with conventional chemical cosmetic compositions.

Therefore, developing new high - performance cosmetics using gold tea will not only inform Korean tea makers around the world, but also lead to national value creation by using high - value - added products.

As a result, it is expected that it will restore trust of customers who have been lost due to various products which had various problems in the efficacy and safety developed in the past and contribute to the improvement of the public health. In the domestic consumption market, By expanding the product range, it is expected that it will be able to protect the consumer market, and furthermore, it will be possible to import substitute functional raw materials and obtain foreign currency through overseas export.

In addition, the developed technology will not be limited to the development of our products, but will enable us to develop various products by sharing the technology with various business partners due to the characteristics of our company. It is expected that the technology developed through such research will be used as an infrastructure technology established as a formal research pattern in developing more functional materials and products in the future.

Claims (8)

A green tea extract containing liposome as an active ingredient,
The gold green tea extract liposome,
(1) dissolving the lipid-stabilizing portion and the hydrophilic activating material at 70 ° C to 80 ° C by dissolving the lipid thickening portion and the lipophilic active material portion at 45 ° C to 50 ° C;
(2) dissolving the lipophilic active material part in the lipid thickening part, adding the lipophilic active material part to the lipid stabilizing part, stirring the mixture at a rotation speed of 1,000 to 2,000 rpm with a stirrer, and uniformly mixing;
(3) adding a portion of the hydrophilic active material to the mixture, stirring the mixture at a rotation speed of 1,000 to 2,000 rpm, and uniformly mixing the mixture; And
(4) forming liposomes by emulsification three to four times using a high-pressure emulsifier,
Wherein the lipid-stabilizing part is composed of 40 wt% of purified water and 1 wt% of a polymer emulsifier and the lipid thickening part is composed of 10 wt% of ethanol, 3 wt% of phospholipid, 0.3 wt% of ceramide and 0.2 wt% of cholesterol, Wherein the hydrophilic active material part is composed of 39% by weight of purified water and 1% by weight of a green tea concentrated powder, wherein the hydrophilic active material part comprises 5% by weight of caprylic / capric triglyceride and 0.5% by weight of lipophilic antioxidant. . delete delete The method according to claim 1,
The gold green tea extract used for preparing the gold green tea extract liposome is,
Wherein the green tea leaves are pulverized, extracted with 20 to 40% ethanol solution at 60 to 100 캜 for 4 to 6 hours, filtered with 0.2 to 5 탆 mesh, and concentrated at 50 to 80 캜. delete The method according to claim 1,
Wherein the gold green tea extract liposome is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a bib cream, a seed cream, a hair tonic, a shampoo, a rinse, a cleansing cream, a cleansing foam, a cleansing water, &Lt; / RTI &gt; delete

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