KR101659655B1 - Soybean-meat containing lower gluten and a preparation method thereof - Google Patents
Soybean-meat containing lower gluten and a preparation method thereof Download PDFInfo
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- KR101659655B1 KR101659655B1 KR1020140115321A KR20140115321A KR101659655B1 KR 101659655 B1 KR101659655 B1 KR 101659655B1 KR 1020140115321 A KR1020140115321 A KR 1020140115321A KR 20140115321 A KR20140115321 A KR 20140115321A KR 101659655 B1 KR101659655 B1 KR 101659655B1
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- soybean
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/05—Mashed or comminuted pulses or legumes; Products made therefrom
- A23L11/07—Soya beans, e.g. oil-extracted soya bean flakes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
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- A23J3/16—Vegetable proteins from soybean
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/22—Working-up of proteins for foodstuffs by texturising
- A23J3/225—Texturised simulated foods with high protein content
- A23J3/227—Meat-like textured foods
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/30—Removing undesirable substances, e.g. bitter substances
- A23L11/31—Removing undesirable substances, e.g. bitter substances by heating without chemical treatment, e.g. steam treatment, cooking
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
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- C—CHEMISTRY; METALLURGY
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- Chemical & Material Sciences (AREA)
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Abstract
본 발명에 의하면 콩고기 결착제로 사용되는 글루텐 함량을 감소시키는 대신에 녹두 분말과 트랜스글루타미아제를 사용하여 조직감 등의 기호성 및 항산화 활성이 우수한 콩고기(콩소시지, 콩핫바 및 콩패티) 및 그 제조방법이 제공된다.According to the present invention, soybean meat (bean sausage, soya bean and soybean patty) and soy sauce (soy bean paste) which are excellent in palatability and antioxidant activity such as texture are used by using mung bean powder and transglutaminase instead of reducing gluten content used as a soya meat binder A manufacturing method is provided.
Description
본 발명은 저 글루텐 함유 콩고기 및 그 제조방법에 관한 것으로서, 더 상세하게는 녹두 분말과 트랜스글루타미아제를 조합하여 사용함에 의해, 물성, 기호성과 항산화성이 우수하면서도 콩고기 결착제로 사용되는 글루텐 함량을 감소시켜 글루텐 알러지 및 소아 지방변증의 유발이 저감된 콩고기 및 그 제조방법에 관한 것이다
The present invention relates to a low-gluten-containing soybean meat and a method for producing the same, and more particularly, to a soybean-containing soybean meal which is excellent in physical properties, palatability and antioxidant properties, Which is reduced in the content of gluten allergen and pediatric fat allergies, and a method for producing the same
콩은 우수한 식물성 단백질 급원으로 특히 한국 사람에게는 두부, 장류, 두유, 콩기름 등의 식품의 형태로 소비되고 있다. 콩은 영양학적 측면뿐만 아니라 사포닌(saponin), 이소플라본(isoflavone), 레시틴(lecithin)와 같은 생리활성 물질에 관한 연구가 활발해지면서 항암, 항동맥경화, 혈당강화 등의 건강기능성 효과가 주목되어 기능성 식품으로 각광받고 있다.Soybean is an excellent source of vegetable protein, and is consumed in the form of foods such as tofu, soy sauce, soy milk and soybean oil, especially for Korean people. As the research on physiologically active substances such as saponin, isoflavone and lecithin as well as the nutritional aspects of soybeans becomes active, attention is paid to health functional effects such as anti-cancer, anti-arteriosclerosis, It is popular as food.
콩 및 콩으로부터 추출한 분리 대두 단백질에 결착제인 글루텐과 여러 가지 부재료를 혼합하여 1차 가공 처리하여 육질과 유사한 섬유조직을 가진 콩고기를 제조할 수 있다. 현재 국내 채식인구는 1% 정도로, 최근에 채식전문식당, 채식뷔페, 채식자연식품점 등에서 콩고기를 판매하고 있으나 아직은 일반화되지 못하고 있고 메뉴의 다양성도 부족한 상태이다. 국내에서 식물성 단백식품의 시장의 매출규모는 연간 약 60-70 억원으로 예측되나 그 규모가 점차 늘고 있는 것으로 보인다.Soybean protein extracted from soybean and soybean can be mixed with gluten, which is a binder, with various materials and subjected to primary processing to produce a soybean protein having a fiber structure similar to that of meat. At present, the domestic vegetarian population is about 1%. Recently, they have been selling Congogi in vegetarian restaurants, vegetarian buffets and vegetarian natural food stores, but they have not been generalized yet. In Korea, the sales of vegetable protein food market is estimated to be around KRW6-7bn per year, but the scale is gradually increasing.
글루텐(gluten)은 밀 같은 곡물에서 발견되는 단백질의 일종인데, 이 글루텐은 알레르겐으로 작용하여 글루텐 알러지를 유발할 수 있고 일명 셀리아크병으로 불리는 소아 지방변증 (celiac disease)의 발병원인이 되기도 한다. 특히 셀리아크병은 서양에서는 전 인구의 0.5 ~ 1.0% 정도가 해당되며, 글루텐을 섭취 시 설사와 구토를 하는 것은 물론 배에 가스가 차고 대변이 회색으로 변하며 심하면 쇼크 상태에 빠지기도 한다. 따라서 콩고기를 제조시에 결착제로서의 글루텐을 대체할 수 있는 다른 식물성 결착제를 사용한 저 글루텐 함유 콩고기 개발의 필요성이 대두되어 왔다.Gluten is a kind of protein found in grains such as wheat, which acts as an allergen, which can cause gluten allergy and cause celiac disease, also known as celiac disease. Celiac disease, especially in the West, accounts for about 0.5% to 1.0% of the population, and it consumes diarrhea and vomiting when the gluten is ingested, as well as gas on the stomach and the stool becomes gray. Therefore, there has been a need to develop low-gluten-containing soybean meats using other vegetable binders that can replace gluten as a binder in the production of soybean curd refuse.
콩고기 제조방법에 관련된 종래기술로는 대두취를 제거한 식물성 고기의 제조방법 및 이에 의해 제조된 식물성 고기에 관한 한국등록특허 10-1115578호, 식물성 고기의 조성물 및 그의 제조방법에 관한 한국등록특허 10-1296186호, 육류대체 조리용 콩고기 및 그 제조방법에 관한 한국공개특허 10-2013-0061462호 등이 있는데, 이들은 주로 콩 분말 및/또는 대두 단백질에 결착제인 글루텐을 20 ~ 30%의 많은 양을 사용하여 콩고기를 제조하는 기술이었다. 따라서 이들 방법에 의해 제조된 콩고기는 과량의 글루텐을 함유하고 있어서 글루텐 알러지나 소아 지방변증을 유발할 수 있었다.Conventional techniques related to soybean meat manufacturing method include a method of producing vegetable meat without soybean sprouts, Korean Patent No. 10-1115578 on vegetable meat produced thereby, Korean Patent No. 10 Korean Patent Laid-Open No. 10-2013-0061462, which relates to a method for preparing soybean meat for substituting meat for meat, and the like, which can be used in a large amount of 20 to 30% of gluten, which is a binding agent to soybean powder and / Was used to produce Congong. Therefore, the congo group produced by these methods contained excess gluten, which could lead to gluten allergy and hypoglycemia.
따라서 콩고기 결착제로 사용되는 글루텐 함량을 감소시켜 글루텐 알러지 및 소아 지방변증의 유발을 저감시킬 수 있고, 콩고기의 저작성, 견고성 및 검성 등의 물성 및 기호성이 향상되고 항산화성도 우수한 콩고기의 제조방법의 개발이 요구되고 있는 실정이다.
Therefore, it is possible to reduce the gluten content used as a soya meat binder and to reduce the induction of gluten allergy and hypocholesterolemia, and to improve the properties and palatability of soybean meat, The development of a method is required.
종래 기술에서의 요구에 부응하기 위해 계속하여 연구한 결과, 본 발명자들은 녹두 분말과 트랜스글루타미아제를 조합하여 첨가함에 의해, 글루텐 함량을 현저히 감소시키고 고기의 저작성, 견고성 및 검성 등의 물성 및 기호성은 우수하면서도 항산화 활성은 증진된 콩고기를 개발하게 되어 본 발명을 완성하기에 이르렀다.As a result of continuing research in order to meet the demand in the prior art, the present inventors have found that by adding a combination of mung bean powder and transglutaminase, the gluten content is remarkably reduced and the physical properties such as meat durability, The present inventors have developed the Congo group having excellent anticorrosive activity and enhanced antioxidative activity, thus completing the present invention.
따라서 본 발명의 목적은 글루텐 함량이 감소되어 글루텐 단백질에 의해 유래될 수 있는 알러지 및 소아 지방변증의 유발을 저감시키고, 아울러 물성 및 기호성도 우수하고 항산화 활성이 증진된 콩고기 및 그 제조방법을 제공하는 것이다.
Accordingly, an object of the present invention is to provide a soybean meat having reduced antioxidative activity, which is reduced in the content of gluten and reduced in the induction of allergic and childhood fatigue, which can be induced by gluten protein, .
상기 목적을 달성하기 위하여, 본 발명은 고형분 전체 중량을 기준으로 증자콩 분말 30 ~ 52.9 중량%, 생콩 분말 9 ~ 15 중량%, 녹두 분말 8 ~ 16 중량%, 트랜스글루타미나아제 0.1 ~ 1.0 중량%, 글루텐 5 ~ 9 중량%, 부재료 25 ~ 39 중량%를 포함하여 이루어지는 콩고기를 제공한다.
In order to attain the above object, the present invention provides a method for producing a soybean meal comprising 30 to 52.9% by weight of an expanded soybean powder, 9 to 15% by weight of a soybean meal, 8 to 16% by weight of a green meal, 0.1 to 1.0% by weight of a transglutaminase % Of gluten, 5 to 9 wt% of gluten, and 25 to 39 wt% of ingredient.
증자콩 분말Increased soybean powder
본 발명의 콩고기는 증자콩 분말을 고형분 전체 중량을 기준으로 30 ~ 52.9 중량%로 포함한다. 증자콩 분말의 함량이 30 중량% 미만이면 콩 비린내가 날 수 있고, 52.9 중량% 초과이면 견고성, 씹힘성(저작성) 및 검성 등의 기호성이 나빠진다.Congogroup of the present invention includes 30 to 52.9% by weight based on the total weight of the solid content of the expanded soybean powder. If the content of the expanded soybean powder is less than 30% by weight, soybean intestine can be produced. If the content is more than 52.9% by weight, the palatability such as firmness, chewiness (low preparation) and gumminess is deteriorated.
증자콩은 콩을 물에 불려서 물만 버리고 물기를 제거하고 100℃에서 1 ~ 2시간 증자하여 제조한다. 증자 시간이 1시간 미만이면 콩의 비린 맛이 완전히 제거되지 않으며, 2시간 초과시에는 너무 심한 단백질 변성으로 콩고기 가공적성이 좋지 않다.The soybean beans are produced by removing the water from the soybeans by soaking in water, and then adding them at 100 ° C for 1 to 2 hours. If the increase time is less than 1 hour, the bean tastes are not completely removed. If the increase time exceeds 2 hours, the bean meat processing ability is not good due to excessive protein denaturation.
콩을 물에 불리는 과정은 당업자에게 용이한 방법으로 이루어질 수 있다. 바람직하게는 콩을 흐르는 수돗물에 3회 세척하여 통상적인 이물질을 제거하며 물기를 제거한 후에, 2배 ~ 3배 분량으로 물을 가하여 20 ~ 30℃에서 8 ~ 16시간 침지하여 불리는 것이 좋으나 이에 제한되지는 않는다.The process of soaking the beans in water can be accomplished in a manner that is readily available to those skilled in the art. Preferably, the beans are washed three times in tap water flowing through the beans to remove common impurities, and then water is added to the beans at a temperature ranging from 20 to 30 ° C for 8 to 16 hours by adding water in an amount of 2 to 3 times, .
증자된 콩은 50 ~ 60℃에서 2 ~ 3 일간 건조시켜서 통상의 분쇄기를 이용하여 100 메쉬 이하로 분쇄하여 분말로 만들 수 있다. 50℃ 미만에서 건조시 건조 시간이 많이 소요되며 60℃ 초과에서 건조시에는 단백질 변성으로 콩고기 가공적성이 좋지 않다. 분말의 크기는, 이에 제한되지는 않지만, 100 메쉬 이하 분말의 경우 콩고기 가공 즉, 반죽이 용이하다.
The expanded beans can be dried at 50 to 60 ° C for 2 to 3 days and then pulverized to a size of 100 mesh or less using a conventional pulverizer to obtain a powder. Drying time at 50 ° C is too high, and when it is dried at 60 ° C or higher, protein denaturation results in poor soybean meat processing ability. The size of the powder is not limited thereto, but in the case of a powder of 100 mesh or less, bean meat processing, i.e., kneading is easy.
생콩 분말Sweet potato powder
본 발명의 콩고기는 생콩 분말을 고형분 전체 중량을 기준으로 9 ~ 15 중량%로 포함한다. 생콩 분말의 함량이 9 중량% 미만이면 견고성 및 씹힘성이 좋지 않고 15 중량% 초과이면 콩 비린내가 날 수 있다.The Congo machine of the present invention comprises soy bean powder in an amount of 9 to 15% by weight based on the total weight of solids. If the content of the soy sauce powder is less than 9% by weight, the firmness and chewiness are not good, and if it is more than 15% by weight, the soybean paste may be produced.
생콩 분말의 제조는 콩을 흐르는 수돗물에 3회 세척하여 통상적인 이물질을 제거한 후 건조시켜 통상의 분쇄기를 이용하여 100 메쉬 이하로 분쇄하여 만들 수 있다. 분말의 크기는, 이에 제한되지는 않지만, 100 메쉬 이하 분말의 경우 콩고기 가공 즉, 반죽이 용이하다.
The soybean powder can be prepared by washing the beans with tap water three times, removing the foreign substances, drying and pulverizing the soybean powder to a size of 100 mesh or less using a conventional pulverizer. The size of the powder is not limited thereto, but in the case of a powder of 100 mesh or less, bean meat processing, i.e., kneading is easy.
녹두 분말Mung bean powder
본 발명의 콩고기는 녹두 분말을 고형분 전체 중량을 기준으로 8 ~ 16 중량%로 포함한다. 녹두 분말의 식물성 전분은 콩단백질 결착제로 작용하여 콩고기에 견고성과 씹힘성을 부여하고 항산화 활성을 증대시킨다. Congogroups of the present invention include mung bean powder in an amount of 8 to 16% by weight based on the total weight of solids. The vegetable starch of mung bean powder acts as a soybean protein binder, conferring firmness and chewiness to the congo plant and enhancing antioxidant activity.
녹두 분말의 함량이 8 중량% 미만이면 결착성 나빠지고, 16 중량% 초과이면 쓴맛에 의해 기호성이 나빠진다. When the content of the mung bean powder is less than 8% by weight, the binding property deteriorates. When the content is more than 16% by weight, the bitterness deteriorates the palatability.
녹두 분말의 제조는 녹두를 흐르는 수돗물에 3회 세척하여 통상적인 이물질을 제거한 후 100 ~ 120℃에서 30 ~ 60 분간 증자하여 건조시켜 통상의 분쇄기를 이용하여 분쇄하여 만들 수 있다. 분말의 크기는, 이에 제한되지는 않지만, 100 메쉬 이하 분말의 경우 콩고기 가공 즉, 반죽이 용이하다.
The mungbean powder can be prepared by washing three times with tap water flowing through mung bean to remove common impurities and then drying at 100 to 120 ° C for 30 to 60 minutes, followed by drying and pulverizing using a conventional mill. The size of the powder is not limited thereto, but in the case of a powder of 100 mesh or less, bean meat processing, i.e., kneading is easy.
트랜스글루타미나아제Transglutaminase (TG) (TG)
본 발명의 콩고기는 트랜스글루타미나아제를 고형분 전체 중량을 기준으로 0.1 ~ 1.0 중량%로 포함한다. The Congo group of the present invention includes transglutaminase in an amount of 0.1 to 1.0% by weight based on the total weight of the solid.
트랜스글루타미나아제는 콩단백질의 재가교 형성을 통하여 콩고기에 견고성과 씹힘성을 부여하여 육고기와 비슷한 질감을 부여할 수 있다. Transglutaminase is able to impart texture and texture to beef meat by imparting rigidity and chewiness to the soybean protein through the ashybridization of soy protein.
트랜스글루타미나아제의 함량은 식품위생법 기준 식품첨가물 허용 범위인 1.0 중량% 이하로 첨가할 수 있으며, 바람직하게는 0.1 ~ 1.0 중량%로 포함된다. 트랜스글루타미나제 함량이 0.1 중량% 미만이면 결착성이 나빠지고, 1.0 중량% 이상은 식품위생법상 첨가 불가능하다.
The content of transglutaminase can be added in an amount of not more than 1.0% by weight, preferably 0.1 to 1.0% by weight, which is an allowable range of food additives according to the Food Sanitation Act. When the content of transglutaminase is less than 0.1% by weight, the binding property is deteriorated. When the content of transglutaminase is less than 0.1% by weight, it can not be added in the food hygiene law.
글루텐gluten
본 발명의 콩고기는 글루텐을 고형분 전체 중량을 기준으로 5 ~ 9 중량%로 포함한다. 글루텐은 콩고기 제조시 주로 사용되어온 콩단백질 결착제로서, 콩고기에 견고성과 씹힘성을 부여하여 육고기와 비슷한 질감을 갖게 한다. The Congo group of the present invention contains gluten in an amount of 5-9% by weight based on the total weight of the solid. Gluten is a soy protein binding agent that has been used mainly for the production of soybean meat, and it imparts firmness and chewiness to the soybean congee so that it has a texture similar to that of meat.
본 발명에 따른 콩고기에는, 상기에 기재된 바와 같이 녹두 분말과 트랜스글루타미나제의 조합이 첨가됨에 따라서, 글루텐이 9 중량% 이하로 포함되어, 글루텐 알러지 및 소아 지방변증의 유발을 저감시킬 수 있다.
As described above, the congong according to the present invention, when the combination of mung bean powder and transglutaminase is added as described above, contains less than 9% by weight of gluten and can reduce the induction of gluten allergy and pediatric adiposity have.
부재료Part fee
본 발명의 콩고기는 부재료를 고형분 전체 중량을 기준으로 25 ~ 39 중량%로 포함한다. Congogroups of the present invention comprise 25 to 39% by weight, based on the total weight of solids, of the material.
부재료는 콩고기의 제조에 통상적으로 사용되는 전분, 채소, 견과류, 버섯 및 소금 등을 포함하는 의미이나, 이에 제한되지는 않는다. The material includes, but is not limited to, starch, vegetables, nuts, mushrooms, salt and the like conventionally used in the production of soybean meat.
전분은 콩고기의 결착성을 부여하는 역할을 하며, 옥수수, 감자, 고구마, 녹두, 팥 전분 등을 사용할 수 있다. Starch serves to bind the bean meat, and corn, potato, sweet potato, mung bean, and red bean starch can be used.
채소로는 양파, 당근, 부추, 파 등이 있으나, 이에 제한되지는 않으며, 잘게 다져서 사용하는 것이 좋다. Vegetables include, but are not limited to, onions, carrots, leeks, and leeks.
견과류로는 땅콩, 아몬드, 호두, 해바라기씨 등이 있으나, 이에 제한되지는 않으며, 으깨거나 분말로 만들어서 사용하는 것이 좋다. Nuts include, but are not limited to, peanuts, almonds, walnuts, and sunflower seeds.
버섯은 말려서 100 메쉬 이하로 분말화하여 사용하는 것이 좋으나, 이에 제한되지는 않는다. The mushroom is preferably dried and powdered to 100 mesh or less, but is not limited thereto.
본 발명의 콩고기에서 부재료를 구성하는 각각의 재료는 고형분 전체 중량을 기준으로 전분 5 ~ 6.5 중량%, 채소 13 ~ 20 중량%, 견과류 5 ~ 9 중량%, 버섯 분말 1.0 ~ 2.0 중량%, 및 소금 1.0 ~ 1.5 중량%으로 포함될 수 있으나, 이에 제한되지 않으며, 통상의 기술자가 소비자의 기호에 맞춰서 적절히 조정할 수 있다.
Each material constituting the ingredient in the bean meats of the present invention contains 5 to 6.5% by weight of starch, 13 to 20% by weight of starch, 5 to 9% by weight of nuts, 1.0 to 2.0% by weight of mushroom powder, And 1.0 to 1.5% by weight of salt, but the present invention is not limited thereto and can be suitably adjusted according to the taste of the consumer by an ordinary technician.
콩고기의 제조Manufacture of bean meat
상기 고형분 재료 즉, 증자콩 분말, 생콩 분말, 녹두 분말, 트랜스글루타미나아제, 글루텐 및 부재료에 육수를 첨가하여 혼합하여 반죽한다. The solid material, that is, the soybean powder, the soybean powder, the green soybean powder, the transglutaminase, the gluten, and the sub ingredient are added to the solid material, and the mixture is kneaded.
본 발명에서 육수는 콩고기 제조에 사용되는 통상의 육수이면 모두 사용가능하며, 바람직하게는 물에 무, 파, 양파, 건표고버섯, 건다시마, 월계수 잎의 식물성 재료를 넣어 100℃에서 1시간 끓여 완성된 야채 육수를 사용한다.In the present invention, the broth can be used as any of ordinary broth used for producing soybean meat. Preferably, the broth is prepared by adding vegetable materials such as radish, onion, onion, dried mushroom, Boil the finished vegetable broth.
고형분 재료 100 중량부에 육수를 80 ~ 90 중량부로 첨가하여 반죽할 수 있으나 이에 제한되지 않으며, 부재료의 종류에 따라서 육수의 첨가량은 변화될 수 있다. In the case of 100 parts by weight of the solid material, 80 to 90 parts by weight of broth may be added and kneaded. However, the addition amount of broth may vary depending on the kind of the filler.
반죽은 통상적으로 사용되는 반죽기를 사용하여 0.5 ~ 1 시간 동안 한다. 고형분 재료 모두를 혼합한 후 육수를 첨가하여 반죽할 수도 있고, 채소를 제외한 고형분 재료를 혼합하여 육수를 첨가하여 1차 반죽한 후, 채소를 혼합하여 2차 반죽할 수도 있다. The kneading is carried out using a conventional kneader for 0.5 to 1 hour. After all of the solid material is mixed, the soup may be kneaded and kneaded. Alternatively, the solid ingredients other than the vegetable may be mixed, and the soup may be added to the kneaded mixture.
반죽물은 4 ~ 8℃ 에서 4 ~ 6 시간 숙성시켜 콩고기를 완성한다. 숙성은 수분이 콩고기에 고르게 융화되게 하기 위함이며, 상온에서 숙성 시 미생물 등이 번식할 수 있어 상기와 같은 냉장온도에서 숙성을 시킨다.
The dough is aged at 4 ~ 8 ℃ for 4 ~ 6 hours to complete Congogi. The fermentation is to make the water uniformly mixed with the soybean flour during fermentation, and when fermented at room temperature, microorganisms and the like may propagate and aged at the above-mentioned refrigeration temperature.
따라서 본 발명의 또 다른 목적을 위하여, 본 발명은 Therefore, for another object of the present invention,
ⅰ) 증자콩 분말 30 ~ 52.9 중량%, 생콩 분말 9 ~ 15 중량%, 녹두 분말 8 ~ 16 중량%, 트랜스글루타미나아제 0.1 ~ 1.0 중량%, 글루텐 5 ~ 9 중량%, 부재료 25 ~ 39 중량%로 구성되는 고형분 재료를 제공하는 단계; I) from 30 to 52.9% by weight of the expanded soybean powder, 9 to 15% by weight of soybean powder, 8 to 16% by weight of mung bean powder, 0.1 to 1.0% by weight of transglutaminase, 5 to 9% by weight of gluten, % By weight of the solid material;
ⅱ) 고형분 재료에 육수를 혼합하여 반죽하는 단계; 및Ii) kneading the solid material with the broth; And
ⅲ) 반죽물은 4 ~ 8℃ 에서 4 ~ 6 시간 숙성시키는 단계를 포함하여 이루어지는 저 글루텐 함유 콩고기의 제조방법을 제공한다.Iii) aging the kneaded product at 4 to 8 ° C for 4 to 6 hours.
본 발명의 제조방법에서 증자콩 분말, 생콩 분말, 녹두 분말, 트랜스글루타미나제, 글루텐 및 부재료는 상기에서 설명된 바와 같다. In the production method of the present invention, the soybean powder, soybean powder, mung bean powder, transglutaminase, gluten, and the sub ingredient are as described above.
본 발명의 제조방법에 사용되는 콩의 종류에는 제한이 없으며, 고단백콩인 새단백콩도 사용할 수 있다. There is no limitation on the kind of beans used in the production method of the present invention, and new protein beans which are high-protein beans can also be used.
본 발명의 제조방법은, 필요에 따라서, 단계 ⅲ) 후에, ⅳ) 콩고기를 원하는 형태의 성형틀에서 성형하는 단계를 추가로 포함할 수 있다.The production process of the present invention may further comprise, if necessary, after step (iii), (iv) molding the Congo into a mold of a desired type.
성형틀은 통상적인 핫바(어묵), 패티 및 소시지 형태로 사용한다.The mold is used in the form of regular hot bar (fish cake), patty and sausage.
또한 본 발명의 제조방법은, 필요에 따라서, 단계 ⅲ) 또는 ⅳ) 후에, 콩고기를 95 ~ 100℃ 에서 0.5 ~ 1 시간 추가 증자할 수 있다. 추가 증자를 통해서 전분, 채소 등을 완전히 익힐 수 있다.Further, in the production method of the present invention, Congogen can be further added at 95 to 100 ° C for 0.5 to 1 hour after step iii) or iv), if necessary. It is possible to fully acquire starch, vegetables, etc. through additional capital increase.
본 발명의 또 다른 목적을 위하여, 본 발명은 상기 제조방법에 따라 제조된, 저 글루텐 함유 콩고기로서, 글루텐 알러지 및 소아 지방변증의 유발이 저감되고, 기호성 및 영양학적이 우수하고, 항산화 활성이 증진된 콩고기를 제공한다. For another object of the present invention, the present invention relates to a low gluten-containing soybean meat produced according to the above-described method, wherein gluten allergy and hypocholesterolemia are reduced, palatability and nutritional properties are improved, Congo.
본 발명에 따른 콩고기는 증자콩 분말 및 생콩 분말을 혼합하여 사용하고, 결착제로서 녹두 분말과 트랜스글루타미나제를 조합하여 사용함에 의해, 글루텐 함량을 콩고기 전체 중량 기준으로 최소 5 %까지 낮추면서도 물성과 기호성이 향상되고 항산화 활성이 증진된다.
The congo group according to the present invention can be obtained by mixing soybean powder with soybean powder and soybean powder and using a combination of mung bean powder and transglutaminase as a binding agent to lower the gluten content to at least 5% But the physical properties and palatability are improved and the antioxidant activity is enhanced.
본 발명의 콩고기는 저작성, 견고성 및 검성 등의 물성과 기호성이 우수하고 항산화 활성이 증진되어 현대의 건강 지향적 트렌드에 부합되는 육고기 대체 식물성 단백질 급원인 콩고기이다.The Congo group of the present invention is soybean meat which is a substitute for vegetable protein in meat, which is excellent in properties and palatability such as low preparation, firmness and gumminess, and antioxidant activity is enhanced and conforms to modern health-oriented trend.
본 발명에 따른 콩고기는 콩핫바, 콩패티, 콩어묵 및 콩소시지의 다양한 형태로 제조할 수 있다.The Congo machine according to the present invention can be manufactured in various forms of soybean hot bar, soybean patty, soybean fish cake and bean sausage.
또한, 본 발명의 제조방법에 의하면, 저작성, 견고성 및 검성 등의 물성이 개선되어 기호성이 향상되고, 우수한 영양학적 특성을 갖고 더불어 글루텐 단백질에 의해 유발되는 글루텐 알러지 및 소아 지방변증이 저감될 수 있는 콩고기를 얻을 수 있다.
Further, according to the production method of the present invention, properties such as low preparation, firmness and gelling are improved, palatability is improved, excellent nutritional characteristics are obtained, and gluten allergy and pediatric adiposity caused by gluten protein can be reduced You can get the Congo.
도 1은 새단백콩을 포함한 5종 콩품종을 사용하여 본 발명의 제조방법에 따라 제조된 콩고기를 보여주는 사진이다. A는 콩핫바 사진이며, B는 콩패티 사진이며, C는 콩소시지 사진이다.Fig. 1 is a photograph showing a congo group produced according to the method of the present invention using five soybean varieties including new protein soybean. A is a picture of bean hot bar, B is bean patty picture, and C is bean sausage picture.
다음의 실시예들에 의해 본 발명이 더 상세히 설명된다. 이들 실시예는 본 발명을 예시하기 위한 것이며, 본 발명의 범위가 이들에 의해 제한되어서는 안된다.
The present invention will be described in more detail by the following examples. These examples are for illustrating the present invention, and the scope of the present invention should not be limited by them.
실 시 예Example
제조예Manufacturing example : 저 글루텐 콩고기 제조: Low gluten bean meat production
<콩 분말 준비><Preparation of soybean powder>
2012년에 수확된 국산콩(새단백콩)은 농촌진흥청 국립식량과학원 기능성작물부(대한민국, 밀양시)에서 공급받아 사용하였다. 콩을 흐르는 수돗물에 3회 세척하여 물기를 제거하였다. 물기를 제거한 원료콩은 50 ~ 60℃에서 1 일간 건조시켰다. Domestic beans (new protein beans) harvested in 2012 were supplied from the Functional Crop Department of the National Institute of Food and Nutrition, Rural Development Administration (Milyang City, Korea). The beans were washed three times in running tap water to remove water. The raw soybeans with moisture removed were dried at 50 ~ 60 ℃ for 1 day.
생콩 분말은 건조된 원료콩을 분쇄기로 80메쉬 이하의 분말로 분쇄하여 준비하였다. The raw soybean powder was prepared by pulverizing the dried raw soybean into a powder having a size of 80 mesh or less by a pulverizer.
증자콩 분말은, 원료콩에 2.5배 분량의 물을 가하고 약 25℃에서 12시간 침지하고 100 메쉬 체로 30분간 물기를 뺀 후, 증자 솥에 넣어 100℃에서 1.5시간 증자한 후, 증자된 콩을 50 ~ 60℃에서 2 ~ 3 일간 건조시키고, 건조된 콩을 분쇄기를 사용하여 80메쉬 이하의 분말로 분쇄하여 준비하였다.
To the soybean powder, a 2.5-fold amount of water was added to the raw soybeans, and the mixture was immersed at 25 ° C for 12 hours, and the water was removed with a 100-mesh sieve for 30 minutes. The soybean powder was then added to the pot and heated at 100 ° C for 1.5 hours. Dried at 50 to 60 DEG C for 2 to 3 days, and the dried soybean was pulverized into a powder having a size of 80 mesh or less by using a pulverizer.
<녹두 분말 준비><Preparation of mung bean powder>
2012년에 수확된 녹두는 농촌진흥청 국립식량과학원 기능성작물부(대한민국, 밀양시)에서 공급받아 사용하였다. 녹두를 흐르는 수돗물에 3회 세척한 후 2배 분량의 물을 가하고 약 25℃에서 4시간 침지하고 100 메쉬 체로 30분간 물기를 뺀 후, 증자 솥에 넣어 100℃에서 1시간 증자한 후, 증자된 콩을 50 ~ 60℃에서 2 ~ 3 일간 건조시키고, 건조된 녹두를 분쇄기를 사용하여 80메쉬 이하의 분말로 분쇄하여 준비하였다.
The mung bean harvested in 2012 was supplied by the Functional Crop Department of the National Institute of Rural Development (Milyang City, Republic of Korea), RDA. After washing three times with tap water flowing in mung bean, water of 2 times amount was added, and the mixture was immersed at about 25 ° C for 4 hours, and the water was removed with a 100 mesh sieve for 30 minutes. Then, the water was added to the pot and heated at 100 ° C for 1 hour. The soybeans were dried at 50 to 60 DEG C for 2 to 3 days, and the dried mung bean was pulverized into powders of 80 mesh or less using a pulverizer.
<글루텐과 트랜스글루타미나제> ≪ Gluten and transglutaminase >
글루텐은 베지푸드 제조사의 활성글루텐을 구입하였고 트랜스글루타미나제는 Shanghai Kinry Food Ingredients 사의 TG-M 제품을 구입하여 사용하였다.
Gluten was purchased from Begifood's manufacturer, and transglutaminase was purchased from Shanghai Kinry Food Ingredients Co., Ltd. (TG-M).
<육수 준비><Preparation of broth>
물 6,000 mL에 무 800 g, 파 150 g, 양파 360 g, 건표고버섯 30 g 및 건다시마 60 g을 넣고 100℃에서 1시간 끓여 냉각한 고형물을 걸러 야채 육수를 제조하였다.
To 800,000 g of water, 150 g of parfum, 360 g of onion, 30 g of onion, 30 g of dried mushroom, and 60 g of dried kelp were added to 6,000 mL of water and boiled at 100 ° C for 1 hour to remove the solids.
<콩고기 제조><Production of soybean meat>
준비된 생콩 분말, 증자콩 분말, 녹두 분말, 트랜스글루타미나아제, 글루텐 및 부재료를 표 1과 같은 함량으로 사용하였다.Prepared soybean powder, soybean powder, soybean powder, mung bean powder, transglutaminase, gluten, and the ingredients were used in the amounts shown in Table 1.
(양파, 당근, 부추, 표고버섯)Chopped vegetables and mushrooms
(Onion, carrot, leek, shiitake mushroom)
다진 채소를 제외한 모든 고형분 재료를 혼합하고, 준비한 채소 육수 500 g을 3회 나누어 첨가하여 반죽기에서 반죽하고, 반죽 마지막 단계에서 다진 채소 (양파: 당근: 부추 : 표고버섯 = 6: 2: 2:1) 160 g을 넣어 다시 재반죽하여 4 ~ 5 시간 동안 숙성하여 콩고기를 제조하였다. All the solid materials except the minced vegetables were mixed and 500 g of the prepared vegetable broth was added in three divided portions and kneaded in a kneader. The minced vegetables (onion: carrot: leek: mushroom = 6: 2: 2: 1 ) Were added, and the mixture was re-kneaded and aged for 4 to 5 hours to prepare congong.
제조된 콩고기는 높이 8cm, 넓이 9.5cm의 성형틀에 넣어 성형한 후 100℃에서 30분간 증자한 후 냉각하여 콩고기를 완성하였다.The congee was molded into a mold having a height of 8cm and a width of 9.5cm, and then formed at 100 ° C for 30 minutes and then cooled to complete the Congo machine.
아울러 동일한 방법으로 새단백콩 5품중을 사용하여 콩핫바, 콩패티 및 콩소시지 형태의 콩고기를 제조하였고 그 사진을 도 1에 나타냈다.
In the same manner, soybean hawba, soybean patty and soy sausage type soybean hulls were prepared from five new protein soybean products, and the photographs thereof are shown in Fig.
참고예Reference example : 콩고기의 추출물 제조: Production of soybean meat extract
제조예에서 제조된 비교예 1, 2 및 실시예 1 ~ 3의 콩고기는 각각 잘게 절단한 후 -70℃에서 이틀간 동결하고 동결건조기를 이용하여 건조한 후 분쇄기로 분쇄하고 분쇄 시료를 5 g을 칭량하고 여기에 10배의 50% 메탄올(50 ml) 가한 후 300 rpm에서 12시간 추출하였다. 추출액을 여과하여 여과액을 얻은 후 여과액 일부는 0.45㎛ 필터로 여과하여 총 페놀릭스 함량 및 총 플라보노이드 함량에 대해 분석하였다. 나머지 여과액 모두를 감압농축기를 이용하여 농축한 후 -70℃에서 하루 정도 동결한 후 동결건조기를 이용하여 건조분말을 얻고, 추출용매인 50% 메탄올을 녹여 각각 0.5, 0.25 및 0.1 mg/ml 농도로 제조하여 항산화 활성을 검정하였다.
Congo samples of Comparative Examples 1 and 2 and Examples 1 to 3 prepared in Preparation Example were finely cut, frozen at -70 ° C for two days, dried using a freeze drier, pulverized by a pulverizer, weighed 5 g of the pulverized sample To this was added 10 times 50% methanol (50 ml) and then extracted at 300 rpm for 12 hours. The extract was filtered to obtain a filtrate, and a part of the filtrate was filtered with a 0.45 μm filter to analyze total phenolix content and total flavonoid content. The remaining filtrate was concentrated using a vacuum concentrator, frozen at -70 ° C for one day, and then dried using a freeze dryer. The extract was dissolved in 50% methanol to give 0.5, 0.25, and 0.1 mg / ml And the antioxidant activity was assayed.
시험예 1. 콩고기의 물성 및 색도, 기호성 평가Test Example 1. Evaluation of physical properties, color and palatability of soybean meat
콩고기의 물성은 비교예 및 실시예의 콩고기를 가로×세로×높이 = 5cm×5cm×1cm로 절단하여 물성기를 이용하여 측정하였고, 그 결과를 표 1에 나타냈다.The physical properties of the soybean meat were measured by cutting the Congo machine of the comparative example and the example of the bean meat with the length × height × height = 5 cm × 5 cm × 1 cm and using the physical property machine, and the results are shown in Table 1.
표 2에 나타낸 바와 같이, 각각의 콩고기 물성을 측정한 결과 비교예 1에 비하여 실시예의 경우가, 견고성은 훨씬 높아졌으며, 비교예 1에 비하여 실시예는 저작성 및 검성이 더 높아졌다. 따라서 본 발명에 따른 콩고기의 물성은 육고기와 더 유사하게 되었다. As shown in Table 2, the physical properties of each soybean meal were measured. As a result, the robustness of the Example was much higher than that of Comparative Example 1, and the preparation and the gumminess of the Examples were higher than those of Comparative Example 1. Therefore, the physical properties of soybean meat according to the present invention became more similar to those of meat.
비교예 및 실시예 콩고기의 색도는 색차계를 이용하여 측정하였고, 그 결과를 표 3에 나타냈다.Comparative Examples and Examples Chromaticity of soybean meat was measured using a color difference meter, and the results are shown in Table 3.
표 3에 나타낸 바와 같이, 각각의 콩고기 색도를 측정한 결과 밝기를 나타내는 L 값, 적색도인 a 값 및 황색도인 b 값은, 녹두 분말의 첨가에 따라서 실시예의 값이 비교예의 값과 차이가 나게 된 것으로 보인다.
As shown in Table 3, each of the bean L value indicating the result of the brightness of measuring the meat color, jeoksaekdo of a value and the yellowness of b value Example values are comparative examples value and the difference made according to, the addition of mung bean powder It seems to have resulted.
시험예 2. 콩고기의 기호성 평가Test Example 2. Evaluation of palatability of soybean meat
비교예 및 실시예의 콩고기의 기호성 평가는 함양농협가공사업소 직원 10명과 경남과학기술대학교 식품과학부 학생 20명의 학생을 대상으로 '매우 좋다 5점, 좋다 4점, 보통이다 3점, 나쁘다 2점, 매우 나쁘다 1점'으로 하는 5점 척도법으로 평가하였고, 그 결과를 표 4에 나타냈다.The palatability evaluation of the bean meats of the comparative example and the example was performed on 10 students of Hamyang Nonghyup processing office and 20 students of the food science department of Kyungnam University of Science and Technology. Very bad 1 point ', and the results are shown in Table 4.
표 4에 나타낸 바와 같이, 콩고기에 대한 기호성 평가를 실시한 결과, 비교예 1의 콩고기보다 실시예의 콩고기에서 맛, 색 및 전체적인 기호도에서 훨씬 향상되었음을 알 수 있다.
As shown in Table 4, it was found that the taste, color, and overall acceptability of bean meats of the example were much improved as compared with that of the bean meats of the comparative example 1 as a result of the palatability evaluation for the Congo period.
시험예Test Example 3. 콩고기의 총 3. Total of bean meat 페놀릭스Phenolics 및 총 플라보노이드 함량 And total flavonoid content
참고예에서 준비된 각각의 시료에 대해서 총 페놀릭스 및 총 플라보노이드 함량을 Folin-Denis법과 Divas법으로 측정하였다.
Total phenolic compounds and total flavonoid contents were determined by Folin-Denis method and Divas method for each sample prepared in Reference Example.
<총 페놀릭스 함량> ≪ Total phenolic content >
참고예에서 준비된 각각의 여과한 액을 100배 희석한 후 0.5 ml을 시험관에 분주하고 25% Na2CO3 용액 0.5 ml을 첨가하여 3분간 정치시켰다. 다시 2N-Folin-Ciocalteu 페놀 시약 0.25 ml 첨가하여 혼합한 다음, 상온에서 1 시간 동안 정치시켜 발색시켰다. 발색된 청색을 분광광도계(Spectronic 2D)를 이용하여 750 nm에서 흡광도를 측정하였다. 이때 총 페놀릭스 함량은 갈산(gallic acid)을 이용하여 작성한 표준곡선으로부터 함량을 구하였고, 그 결과를 표 5에 나타냈다.Each filtrate prepared in Reference Example was diluted 100 times, 0.5 ml was added to a test tube, 0.5 ml of 25% Na 2 CO 3 solution was added, and the mixture was allowed to stand for 3 minutes. Then, 0.25 ml of 2N-Folin-Ciocalteu phenol reagent was added to the mixture, and the mixture was allowed to stand at room temperature for 1 hour to develop color. The color developed blue was measured for absorbance at 750 nm using a spectrophotometer (Spectronic 2D). The content of total phenolics was determined from the standard curves prepared using gallic acid. The results are shown in Table 5.
총 플라보노이드 함량은 참고예에서 준비된 각각의 여과액을 50배 희석한 후 0.5 ml을 시험관에 분주하고 디에틸렌 글리콜 1.0 ml, 1N-NaOH 0.01 ml를 가한 후 37℃ 항온 수조에서 1시간 동안 방치한 후 분광광도계(Spectronic 2D)를 이용하여 420 nm에서 흡광도를 측정하였고, 총 플라보노이드 함량은 루틴(Rutin)을 이용하여 작성한 표준곡선으로부터 함량을 구하고 그 결과를 표 5에 나타냈다.The total amount of flavonoid was determined by diluting 50 times of each filtrate prepared in Referential Example, 0.5 ml of the filtrate prepared in Referential Example was dispensed into a test tube, 1.0 ml of diethylene glycol and 0.01 ml of 1N NaOH were added thereto, and the mixture was allowed to stand in a constant temperature water bath at 37 ° C for 1 hour Absorbance was measured at 420 nm using a spectrophotometer (Spectronic 2D), and the content of total flavonoid was determined from a standard curve prepared using Rutin. The results are shown in Table 5.
(mg/g)Evaluation items
(mg / g)
표 5에 나타낸 바와 같이, 총 페놀릭스 함량은 비교예에 비하여 실시예의 콩고기가 증가하였고, 총 플라보노이드 함량도 비교예에 비하여 실시예의 콩고기가 증가했다.
As shown in Table 5, the content of total phenolics in the Examples was increased compared to the Comparative Examples, and the content of total flavonoids was also increased in the Congo groups of the Examples compared to the Comparative Examples.
시험예Test Example 4. 콩고기의 항산화 활성 검정 4. Antioxidant activity test of soybean meat
항산화 활성은 참고예에서 준비된 시료에 대해 DPPH (1,1-diphenyl-2-picrylhydrazyl) 라디칼 소거활성, ABTS 라디칼 (2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid 양이온) 소거활성, 환원력 (FRAP; Ferric reducing antioxidant power) 활성을 분석하여 검정하였다.
The antioxidant activity of DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, ABTS radical (2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid cation) , And ferric reducing antioxidant power (FRAP) activity.
<DPPH 라디칼 소거활성> ≪ DPPH radical scavenging activity >
DPPH(1,1-diphenyl-2-picrylhydrazyl, Sigma D9132, FW 393.4, C18H12N5O6)는 매우 안정한 라디칼로서 525nm에서 특정 흡광도를 나타내는 보라색 화합물이다. DPPH 라디칼 소거활성을 측정하여 항산화 활성을 확인하였다. DPPH (1,1-diphenyl-2- picrylhydrazyl, Sigma D9132, FW 393.4, C 18 H 12 N 5 O 6) is a purple compound showing a specific absorption at 525nm as a highly stable radicals. DPPH radical scavenging activity was measured to confirm antioxidant activity.
구체적으로는, 참고예에서 준비된 시료 각각 0.2 ml에, 에탄올로 1.5 × 10-4 M 농도가 되게 한 DPPH(1,1-diphenyl-2-picryl-hydrazyl)용액 0.8 ml씩을 보텍스(vortex)로 균일하게 혼합한 다음 실온에서 30분간 방치한 후 525 nm에서 흡광도(O.D.)를 측정하였다. 음성 대조구 실험은 시료 대신에 증류수를 0.2 ml를 취하여 실험하였다. DPPH 라디칼 소거활성은 아래와 같은 식으로 계산하여 백분율(%)로 표시하였으며, 그 결과를 표 6에 나타냈다.Specifically, 0.8 ml of a DPPH (1,1-diphenyl-2-picryl-hydrazyl) solution having a concentration of 1.5 × 10 -4 M in ethanol was added to 0.2 ml of each of the samples prepared in Reference Example in a vortex, The mixture was allowed to stand at room temperature for 30 minutes, and the absorbance (OD) was measured at 525 nm. In the negative control experiment, 0.2 ml of distilled water was used instead of the sample. The DPPH radical scavenging activity was expressed as a percentage (%) by the following formula, and the results are shown in Table 6. < tb >< TABLE >
DPPG 라디칼 소거활성(%) =DPPG radical scavenging activity (%) =
[1-(음성대조구 흡광도 ÷ 실험구 흡광도)] × 100
[1- (negative control absorbance / experimental absorbance)] × 100
(0.5 mg/ml 처리)Evaluation items
(0.5 mg / ml treatment)
표 6에 나타난 바와 같이, 비교예 콩고기에 비하여, 본 발명에 따른 실시예 콩고기의 DPPH 라디칼 소거활성이 증가했다.
As shown in Table 6, the DPPH radical scavenging activity of the soybean meat of the example according to the present invention was increased as compared with that of the Comparative Example Kong Kong.
<ABTs 라디칼 소거활성><ABTs radical scavenging activity>
ABTs 라디칼 소거활성은 2-아지노-비스의 색을 띤 라디칼의 감소 정도에 따라 항산화능을 검사하는 방법이다. 이 방법은 시료와 표준물질(Trolox, 6-hydroxy -2,5,7,8-tetramethylchroman-2-carboxylic acid)의 값과 비교하여 항산화능을 측정하는 방법으로서, 시료의 항산화력에 의해 ABTS 양이온(ABTS)이 소거되어 청록색으로 탈색되는데, 이때 ABTS 양이온(ABTS)의 제거 정도를 흡광도를 측정함으로서 알 수 있고, 탈색반응이 1분 내에 종료되므로 짧은 시간에 측정이 가능하다.The ABTs radical scavenging activity is a method for examining the antioxidant ability of 2-azino-bis according to the degree of reduction of colored radicals. This method is a method to measure the antioxidant ability by comparing with the value of sample and reference material (Trolox, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) (ABTS) is erased and decolorized in cyan. At this time, the degree of ABTS cations (ABTS) can be detected by measuring the absorbance, and the decolorization reaction is completed within 1 minute.
구체적으로 7 mM ABTS 시약 (Sigma 1888) 5 ml과 140 mM K2S2O8 (FW 270.3, Sigma 9392) 5 ml을 섞어 어두운 곳에 14 ~ 16시간 방치시킨 후, 이를 무수 메탄올과 약 1 : 88 비율로 섞어 732 nm에서 대조구의 흡광도 값이 0.70 ± 0.02가 되도록 조절한 ABTS 용액을 사용하였다. 참고예에서 준비한 각각의 시료 0.1 ml과 ABTS 용액 0.9 ml를 혼합하여 30초간 진탕한 후 3분간 반응시키고 732 nm에서 흡광도를 측정하였다. 음성 대조구 실험은 시료 대신에 증류수로 실험하였다. ABTS 라디칼 소거활성은 아래와 같은 식으로 계산하여 백분율(%)로 표시하였으며, 그 결과를 표 6에 나타냈다. Specifically, 5 ml of 7 mM ABTS reagent (Sigma 1888) and 5 ml of 140 mM K 2 S 2 O 8 (FW 270.3, Sigma 9392) were mixed and allowed to stand in the dark for 14 to 16 hours. , And the absorbance of the control was adjusted to 0.70 ± 0.02 at 732 nm. 0.1 ml of each sample prepared in Reference Example and 0.9 ml of ABTS solution were mixed, shaken for 30 seconds, reacted for 3 minutes, and absorbance was measured at 732 nm. Negative control experiments were performed with distilled water instead of samples. The ABTS radical scavenging activity was expressed as a percentage (%) by the following formula, and the results are shown in Table 6.
ABTS 라디칼 소거활성(%) =ABTS radical scavenging activity (%) =
[1-(음성대조구 흡광도 ÷ 실험구 흡광도)] × 100[1- (negative control absorbance / experimental absorbance)] × 100
표 6에 나타난 바와 같이, ABTS 라디칼 소거활성도 비교예 콩고기에 비하여 실시예 콩고기에서 증가함을 확인할 수 있다.
As shown in Table 6, it was confirmed that the ABTS radical scavenging activity was increased in the example soybean meat as compared with the Comparative Example Kongong period.
<환원력 분석><Reducing power analysis>
환원력 분석은 FRAP 분석을 기초로 한 Liu 등의 방법을 응용하여 측정하였다. Reducing power was measured by using Liu et al. Based on FRAP analysis.
구체적으로 FRAP 환원력 분석에서 반응액으로는 300mM 아세테이트 완충액(pH 3.6), 40 mM 염산에 녹인 10 mM 2,4,6-트리피리딜-s-트리아진(TPTZ, T1253, C18H12N6, MW312.33) 및 20mM FeCl3(F7134, MW 162.20)를 준비하였으며, 아세테이트 완충액, TPTZ 용액 및 FeCl3 용액을 10 : 1 : 1 (v/v/v)로 혼합하여 37℃ 에서 15분 간 예비반응을 시켜두었다. 참고예에서 준비된 시료 각각 0.1 ml과 예비반응된 FRAP 시약 0.9 ml을 96-웰 플레이트에 분주한 후 15분간 반응시키고 마이크로플레이트 리더(Biorad 3055, Sweden)를 사용하여 593 nm에서 흡광도를 측정하여 그 결과를 표 6에 나타냈다.Specifically, in the FRAP reduction assay, 10 mM 2,4,6-tripyridyl-s-triazine (TPTZ, T1253, C18H12N6, MW312.33) dissolved in 40 mM acetate buffer (pH 3.6) And 20 mM FeCl 3 (F7134, MW 162.20) were prepared, and the acetate buffer, the TPTZ solution and the FeCl 3 solution were mixed at a ratio of 10: 1: 1 (v / v / v) . 0.1 ml of each sample prepared in Reference Example and 0.9 ml of preliminarily reacted FRAP reagent were dispensed into a 96-well plate, reacted for 15 minutes, and absorbance was measured at 593 nm using a microplate reader (Biorad 3055, Sweden) Are shown in Table 6.
표 6에 나타난 바와 같이, 환원력도 비교예 콩고기에 비하여 본 발명에 따른 실시예 콩고기에서 증가함을 확인할 수 있다.As shown in Table 6, it can be confirmed that the reducing power is also increased in the soybean meat of the example according to the present invention, as compared with that of the Comparative Example Congo.
상기의 결과들로부터 본 발명에 따라 제조된 콩고기는 증진된 항산화 활성을 가짐을 알 수 있다.
From the above results, it can be seen that the Congo group prepared according to the present invention has an enhanced antioxidative activity.
Claims (9)
A solid content of 30 to 52.9% by weight, a soybean powder of 9 to 15% by weight, a green bean flour of 8 to 16% by weight, a transglutaminase of 0.1 to 1.0% by weight, a gluten of 5 to 9% by weight, And 25 to 39% by weight of the ingredient.
The low gluten-containing soybean meat according to claim 1, wherein the sub ingredient contains starch, vegetables, nuts, mushrooms and salt.
The gluten-containing soybean meat according to claim 1, which is improved in physical properties, palatability and antioxidant activity of low-hardness, firmness and gums.
The gluten-containing soybean meat according to claim 1, wherein the total phenolic content and the total flavonoid content are increased.
ⅱ) 고형분 재료에 육수를 혼합하여 반죽하는 단계; 및
ⅲ) 반죽물은 4 ~ 8℃ 에서 4 ~ 6 시간 숙성시키는 단계를 포함하여 이루어지는 저 글루텐 함유 콩고기의 제조방법.
(I) 30 to 52.9% by weight of the expanded soybean powder, 9 to 15% by weight of soybean powder, 8 to 16% by weight of mung bean powder, 0.1 to 1.0% by weight of transglutaminase, 5 to 9% by weight of gluten, % By weight of the solid material;
Ii) kneading the solid material with the broth; And
Iii) aging the kneaded product at 4 to 8 DEG C for 4 to 6 hours.
The method according to claim 6, further comprising, after step iii), iv) molding the congogue in any one of a hot bar, a patty and a sausage.
The method of claim 7, further comprising the step of: after step iii) or iv), further increasing the congo group at 95 to 100 DEG C for 0.5 to 1 hour.
A soybean meat produced according to the production method according to any one of claims 6 to 8 and having reduced gluten content and improved physical properties, palatability and antioxidant activity of chewing, firmness and gums.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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KR20190008785A (en) | 2017-07-17 | 2019-01-25 | 온고을팜 협동조합 | Functional Functional Mushroom Fermented Soymeat Added by Rubus coreanus Miquel and Manufacturing Method the Same |
KR20200127380A (en) | 2019-05-02 | 2020-11-11 | 롯데푸드 주식회사 | A manufacturing method of vegetarian hamburger patty reduced unpleasant taste and smell |
KR20210114908A (en) | 2019-05-02 | 2021-09-24 | 롯데푸드 주식회사 | A manufacturing method of vegetarian hamburger patty reduced unpleasant taste and smell |
KR20220122396A (en) | 2021-02-26 | 2022-09-02 | 박상철 | Non-Animal Meat Substitute |
KR102415737B1 (en) | 2022-01-05 | 2022-06-30 | 박진아 | chicken meat substitute and manufacturing method thereof |
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