KR20190008785A - Functional Functional Mushroom Fermented Soymeat Added by Rubus coreanus Miquel and Manufacturing Method the Same - Google Patents
Functional Functional Mushroom Fermented Soymeat Added by Rubus coreanus Miquel and Manufacturing Method the Same Download PDFInfo
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- KR20190008785A KR20190008785A KR1020170094172A KR20170094172A KR20190008785A KR 20190008785 A KR20190008785 A KR 20190008785A KR 1020170094172 A KR1020170094172 A KR 1020170094172A KR 20170094172 A KR20170094172 A KR 20170094172A KR 20190008785 A KR20190008785 A KR 20190008785A
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- mushroom
- fermented soybean
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- soybean
- mycelium
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Abstract
본 발명은 복분자를 첨가한 기능성 버섯 발효콩고기 및 그 제조방법에 관한 것이다. 보다 상세하게는 버섯 균사체를 이용하여 버섯 발효콩을 만드는 단계와, 복분자로부터 복분자추출액을 얻는 단계와, 정제수에 복분자추출액, 발효콩, 전분, 양파, 버섯, 당근, 참깨, 견과류 및 착즙액을 넣고 믹서로 분쇄한 다음, 부재료와 양념을 넣고 반죽기에서 반죽한 반죽물을 냉장 숙성시켜 콩고기를 만드는 단계와, 상기의 콩고기를 핫바, 패티, 햄, 불고기, 스테이크 및 소시지로 구성되는 어느 하나의 성형 틀에서 성형 및 증숙하는 단계로 구성되는 것을 특징으로 하는 발효콩고기의 제조방법으로 구성된다.
본 발명의 복분자를 첨가한 기능성 버섯 발효콩고기는 다양한 형태로 성형될 수 있으며, 기능성 및 기호성이 우수한 발효콩고기를 제공할 수 있다.The present invention relates to a functional mushroom fermented soybean meat to which bokbunja is added and a method for producing the same. More particularly, the present invention relates to a method for producing a mushroom fermented soybean using mushroom mycelium, a step of obtaining a bokbunja extract from a bokbunja, a step of adding bokbunja extract, fermented soybean, starch, onion, mushroom, carrot, sesame, A step of crushing the mixture with a mixer and then adding the ingredients and the seasoning to make a congee by aging the kneaded product dough kneaded in a kneader; and a step of preparing the congee by mixing the congee with any one of the molds consisting of hotba, patty, ham, bulgogi, steak and sausage And a step of forming and cooking the fermented soybean meat.
The functional mushroom fermentation soybean curd added with the brambles of the present invention can be molded into various forms and can provide a fermented soybean curd superior in functionality and palatability.
Description
본 발명은 복분자를 첨가한 기능성 버섯 발효콩고기 및 그 제조방법에 관한 것이다. 보다 상세하게는 버섯 균사체를 이용하여 버섯 발효콩을 만드는 단계와, 복분자로부터 복분자추출액을 얻는 단계와, 정제수에 복분자추출액, 발효콩, 전분, 양파, 버섯, 당근, 참깨, 견과류 및 착즙액을 넣고 믹서로 분쇄한 다음, 부재료와 양념을 넣고 반죽기에서 반죽한 반죽물을 냉장 숙성시켜 콩고기를 만드는 단계와, 상기의 콩고기를 핫바, 패티, 햄, 불고기, 스테이크 및 소시지로 구성되는 어느 하나의 성형 틀에서 성형 및 증숙하는 단계로 구성되는 것을 특징으로 하는 발효콩고기의 제조방법으로 구성된다.The present invention relates to a functional mushroom fermented soybean meat to which bokbunja is added and a method for producing the same. More particularly, the present invention relates to a method for producing a mushroom fermented soybean using mushroom mycelium, a step of obtaining a bokbunja extract from a bokbunja, a step of adding bokbunja extract, fermented soybean, starch, onion, mushroom, carrot, sesame, A step of crushing the mixture with a mixer and then adding the ingredients and the seasoning to make a congee by aging the kneaded product dough kneaded in a kneader; and a step of preparing the congee by mixing the congee with any one of the molds consisting of hotba, patty, ham, bulgogi, steak and sausage And a step of forming and cooking the fermented soybean meat.
본 발명의 복분자를 첨가한 기능성 버섯 발효콩고기는 다양한 형태로 성형될 수 있으며, 기능성 및 기호성이 우수한 발효콩고기를 제공할 수 있다. The functional mushroom fermentation soybean curd added with the brambles of the present invention can be molded into various forms and can provide a fermented soybean curd superior in functionality and palatability.
본 발명의 복분자를 첨가한 기능성 버섯 발효콩고기는 다양한 형태로 성형될 수 있으며, 기능성 및 기호성이 우수한 콩고기를 제공할 수 있다. The functional mushroom fermented soybean curd with the addition of the brambles of the present invention can be shaped into various forms and can provide a soybean curd refiner having excellent functionality and palatability.
복분자(覆盆子;Rubus coreanus Miquel)는 장미과에 속하는 낙엽관목으로 우리나라의 제주도 및 남부지역이 주요산지로 초여름에 검붉은 열매를 수확하고 있다. 한방에서 복분자는 강장제, 강정제 및 보간의 요약으로서 귀한 약재로 알려져 왔으며, 신체허약, 양위, 유정, 유뇨 등에 이용되어 왔다. 헬리코박터 파일로리균(Helicobacter pylori)의 생육 억제작용, 시상하부-뇌하수체-성선축의 기능을 증강함으로써 난소제거 암컷 흰쥐의 생식내분비기능 부조화로 야기되는 증상에 대해 개선 효과가 있다. 복분자는 한방에서 당뇨병, 성욕감퇴, 정액루, 유뇨증, 천식, 알레르기 관련 치료에 사용되고는 항산화, 혈관신생 억제, 식도암 등에 효과가 보고되었다.Rubus coreanus Miquel is a deciduous shrub belonging to the Rosaceae. It is a major mountain of Jeju and southern part of Korea and harvests sown red fruit in early summer. Bokbunja has been known as a valuable medicine as a summary of tonic, gangjeongje and interpolation in the oriental medicine. It has been used for weakness of the body, immobilization, oil well, urination. Helicobacter pylori growth inhibition and hypothalamic-pituitary-gonadal axis functions are enhanced to improve the symptoms caused by gonadal dysfunction in the ovariectomized female rats. Bokbunja is used in the treatment of diabetes, libido, flatulence, enuresis, asthma, allergy, and antioxidant, angiogenesis inhibition and esophageal cancer.
복분자는 유리당, 무기질의 인, 철 및 칼륨이 많이 함유하여 있고 특히 유기산과 비타민C가 많이 포함되어 있으며, 항암활성 및 면역증진효과, 항산화 및 항균효과, B형간염바이러스 억제 등 다양한 생리활성에 대한 효능이 밝혀진 바 있다. 최근 연구에 의하면, 복분자 물추출물이 동물시험과 임상시험에서 저밀도 지단백(LDL)을 억제함으로써 고지혈증 및 지질대사에 효능이 있음을 확인하였고, 혈관질환의 원인이 되는 염증성 사이토카인인 TNF-a, IL-6, PGF2, 일산화질소 등의 생성을 억제한다고 보고한 바 있다Bokbunja contains a lot of free sugars, inorganic phosphorus, iron and potassium. Especially it contains organic acid and vitamin C. It has various antimicrobial activities such as anticancer activity and immunity enhancing effect, antioxidant and antibacterial effect, Efficacy has been revealed. Recent studies have shown that bokbunja water extract is effective in the treatment of hyperlipidemia and lipid metabolism by inhibiting low density lipoprotein (LDL) in animal studies and clinical trials. The inflammatory cytokines TNF-a, IL -6, PGF2, nitrogen monoxide, and the like
콩(大斗; Soybean)은 콩은 우수한 식물성 단백질 공급원으로 동북아시아인에게는 두부, 장류, 두유, 콩기름 등의 형태로 소비되고 있다. 콩은 수분 7.5%, 단백질 40%, 지방 20%, 회분, 4.5%, 탄수화물 28%가 함유되어 있는 풍부한 영양학적 측면 뿐만 아니라 사포닌(saponin), 이소플라본(isoflavone), 레시틴(lecithin)과 같은 생리활성 물질에 관한 연구가 활발해지면서 항암, 항동맥경화, 혈당강화 등의 건강기능성 효과가 주목되어 기능성 식품으로 각광받고 있다. 콩에 가장 풍부한 아미노산은 글루탐산과 아스파르트산으로 전체 아미노산 량의 1/4을 차지하고 있으며, 가장 적은 아미노산은 트립토판과 메티오닌이다. 콩의 식이섬유는 대부분 불용성으로 평균 26%를 함유하고 있으며, 이외에도 칼륨, 인, 칼슘, 마그네슘 등의 미네랄을 다량 함유하고 있다. 콩의 주요 기능성분 중의 하나인 안토시아닌은 식물의 꽃이나 과실 껍질 등에서 고운 빛깔을 가지는 부분에 많이 존재하며, 주로 적색이나 청색, 자색 등을 나타내는데, 결합하는 화합물의 종류에 따라 여러가지 색상으로 나타난다. 안토시아닌은 천연색소로서의 기능 외에도 다양한 생리활성을 나타냄으로서 최근 주목받고 있다. 현재까지 알려진 생리활성 기능으로는 경구 및 피하지방층에 투여시 항산화, 콜레스테롤 저하, 시력개선효과, 혈관보호 기능, 동맥경화, 심장병 예방, 항궤양기능, 항암, 항염증, 당뇨억제 등이 있다. 콩의 여러 가지 영양성분 중 이소플라본의 아글리콘 함량 증가를 위한 미생물로는 Bacillus를 이용한 청국장, natto균을 이용한 나토, Aspergillus oryzae를 이용한 된장, Rhizophous 균을 이용한 템페(tempeh)가 대표적이다. 우리나라 식품의약품안전처의 권장에 따르면 이소플라본(isoflavone)의 1일 권장 섭취량을 25~27 ㎎/day로 권장하고 있고, 이 양을 콩으로부터 섭취하려면 약 250~270 g/day, 청국장으로 62~67 g/day라고 하는데, 버섯 발효콩 소재의 총 이소플라본은 3.69~5.94 mg/g(daidzein과 genistein으로 3.91~6.35 mg/g)으로 분말 소재의 경우 약 5~7g/day에 해당된다. 한편, 고등 곰팡이로 불리는 버섯(mushroom)은 약 15,000여종이 있고, 그 중 2,000여종이 식용 가능한 것으로 되어 있는데, 우리나라는 970여종이 분포하고 있으며, 이중 350여종이 식용 가능하다고 알려져 있으며, 이 중 인공재배는 20여종이 가능하다. 버섯균은 면역활성 작용, 항암 작용, 항혈전 작용, 항콜레스테롤 작용, 항산화 작용 등 질병 예방에 유효한 효과가 있고, 식이섬유가 많이 함유되어 있어 변비나 비만을 해소하는 등의 약용 효과가 기대되는 기능성 식품으로서 주목받고 있다. 국내외적으로 콩고기를 상품화한 베지푸드 콩고기3종셋트, 쏘이마루 탕수육콩고기, 천일식품 콩단백콩고기, 삼육식품 베지버거, J아람 버건스틱 등이 상품화되어 있으나, 기능성이 결여된 제품들로 구성되어 있다.Soy beans are a good source of vegetable protein and are consumed by Northeast Asian people in the form of tofu, soy sauce, soy milk, and soybean oil. Soybeans are rich in nutritional aspects including 7.5% moisture, 40% protein, 20% fat, ash, 4.5% and 28% carbohydrates as well as physiological aspects such as saponin, isoflavone, lecithin, As the research on active substances becomes active, the health functional effect such as anti-cancer, anti-arteriosclerosis, and blood glucose intensification is attracting attention as a functional food. The most abundant amino acids in soybeans are glutamic acid and aspartic acid, accounting for 1/4 of total amino acids. The smallest amino acids are tryptophan and methionine. Dietary fiber of soybean is mostly insoluble and contains 26% on average, and also contains a large amount of minerals such as potassium, phosphorus, calcium and magnesium. Anthocyanin, one of the main functional components of soybeans, is present in many parts of plants such as flowers and fruit husks, which have fine colors. They mainly show red, blue, and purple colors. Anthocyanins are attracting attention recently as they exhibit various physiological activities in addition to functions as natural pigments. The physiologically active functions known to date include antioxidants, cholesterol lowering, vision improvement, vascular protection, arteriosclerosis, prevention of heart disease, anti-ulcer function, anti-cancer, anti-inflammatory and diabetic suppression when administered to the oral and subcutaneous fat layers. Among the various nutrients of soybean, microorganisms for increasing the aglycon content of isoflavones are Chungkukjang using Bacillus, NATO using natto, Doenjang using Aspergillus oryzae, and Tempeh using Rhizophous bacteria. According to the recommendation of Korea Food and Drug Administration, the recommended daily intake of isoflavone is 25 ~ 27 ㎎ / day, and it is recommended to consume 250 ~ 270 g / day, 67 g / day. Total isoflavone content of mushroom fermented soybean material is 3.69 ~ 5.94 mg / g (3.91 ~ 6.35 mg / g in daidzein and genistein) and about 5 ~ 7 g / day in case of powder material. On the other hand, there are about 15,000 mushrooms called high molds, of which about 2,000 are available for cooking. There are about 970 mushrooms distributed among Korea, and about 350 mushrooms are known to be edible. Among them, There are 20 kinds of cultivation. The mushroom fungus has an effective effect for prevention of diseases such as immunological activity, anticancer activity, anti-thrombotic action, anticholesterol action and antioxidative action, and has a function which is expected to have medicinal effects such as constipation and obesity because it contains a lot of dietary fiber It is attracting attention as food. It is made up of products that are commercialized but not functionally functional, such as beef fudge bean meat, beef meat, soymaru sweet and sour bean meat, chunil food, bean protein bean meat, beef burger and Jaram burger stick commercialized in Korea and abroad. have.
콩고기에 관련된 종래기술로는 한국특허등록번호 10-1514534(콩고기 가공식품 및 이의 제조방법)은 콩 분쇄물 100 중량부에 대하여 글루텐 15 ~ 75 중량부, 견과류 7 ~38 중량부, 전분 5 ~ 40 중량부, 채소 5 ~ 30 중량부, TG 0.1 ~ 2.5 중량부 및 육수 100 ~ 200 중량부 포함하고 항산화 활성을 갖는 콩고기 가공식품. 상기 콩고기 가공식품은 콩고기, 콩소시지, 콩햄, 콩버거패티, 콩까스, 콩불고기 및 콩스테이크로 구성된 군 중 어느 하나 이상인 콩고기 가공식품이다. 한국특허등록번호 10-1397794(기호성과 항산화 활성이 향상된 콩고기의 제조방법)은 i) 콩에 2 ~ 3배 분량으로 물을 가하여 20℃ ~ 30℃ 에서 8 ~ 16시간 침지하는 단계; ii) 물을 제거하고 100℃ 에서 1 ~ 2시간 증자하는 단계; iii) 증자된 콩을 50℃ ~ 60℃ 에서 2 ~ 3 일간 건조시키는 단계; iv) 건조된 콩을 분쇄하여 분말로 만드는 단계; 및 v) 분말 콩에 부재료를 혼합한 후 육수를 첨가하여 반죽한 후 숙성시키는 단계; vi) 콩고기를 성형틀에서 성형하는 단계, 및/또는 vii) 증자하는 단계를 추가로 포함할 수 있다. 한국특허공개번호 10-2017-0055356(공액리놀레산과 이소플라본의 함량이 증대된 콩고기 및 이의 제조 방법)은 (a) 증자콩에 버섯균사체 배양액을 접종하여 발효한 후, 버섯균사체 발효콩 분말을 준비하는 단계; (b) 상기 (a)단계에서 준비한 버섯균사체 발효콩 분말 47.5 ~ 57.5 중량%, 녹두 분말 8 ~ 16 중량%, 전분 2~6 중량%, 글루텐 3 ~ 7 중량%, 트랜스글루타미나아제 0.1 ~ 2.0 중량% 및 부재료 20 ~ 35 중량%으로 구성되는 고형분 재료를 혼합하는 단계; (c) 상기 고형분 재료에 육수를 혼합하여 반죽하는 단계 ; 및 (d) 반죽물은 4 내지 8 ℃에서 4 내지 6 시간 동안 숙성시키는 단계; (e) 콩고기를 핫바, 패티, 햄, 불고기, 스테이크 및 소시지로 구성되는 어느 하나의 성형 틀에서 성형하는 단계를 추가적으로 포함하는 것을 특징으로 하는 공액리놀레산과 이소플라본의 함량이 증대된 콩고기 제조 방법이다. 한국특허등록 10-1659655(저 글루텐 함유 콩고기 및 그 제조방법)은 ⅰ) 증자콩 분말 30 ~ 52.9 중량%, 생콩 분말 9 ~ 15 중량%, 녹두 분말 8 ~ 16 중량%, 트랜스글루타미나아제 0.1 ~ 1.0 중량%, 글루텐 5 ~ 9 중량%, 및 부재료 25 ~ 39 중량%로 구성되는 고형분 재료를 제공하는 단계; ⅱ) 고형분 재료에 육수를 혼합하여 반죽하는 단계; ⅲ) 반죽물은 4 ~ 8℃ 에서 4 ~ 6 시간 숙성시키는 단계, ⅳ) 콩고기를 핫바, 패티 및 소시지로 구성되는 어느 하나의 성형틀에서 성형하는 단계를 추가로 포함하는 것인 저 글루텐 함유 콩고기의 제조방법이 있다. 그 밖에 콩을 이용한 가공제품에 관련된 종래기술로는 콩을 주원료로 한 햄과 소시지 타입의 두류가공품의 제조방법에 관한 한국특허공개번호 10-2014-0143315, 한국특허공개 2001-0054290호, 초 저지방 고급 세절 소시지의 가공기술에 관한 한국특허공개 2004-0076548호, 조리빵용 콩고기 제조방법에 관한 한국특허공개 2010-033718호, 검정콩에서 추출한 단백질과 안토시아닌 추출액을 이용한 콩소시지 및 그의 제조방법에 관한 한국특허등록 10-0936514호 및 조직 콩단백질을 이용한 콩소시지 제조방법에 관한 한국특허등록 10-0878329호 등이 있는데, 이들은 주로 대두 단백질을 이용한 콩고기류 제조 기술이 있다. 또한 발효콩에 관한 종래기술로는 증자콩 또는 증자 발아콩에 유산균을 접종하고 배양하여 콩발효물을 제조하는 방법(공개특허공보 제10-2016-0056489호), 콩가공 부산물을 바실러스 속 미생물로 발효시켜 이소플라본 비배당체의 함량을 증진시키는 방법(공개특허공보 제10-2013-0056020호), 증자한 콩나물에 바실러스, 곰팡이 또는 효모를 접종하고 배양하여 콩나물 발효식품을 제조하는 방법(공개특허공보 제10-2007-0113674호) 등이 개시되어 있다.Korean Patent Registration No. 10-1514534 (soybean meat processed food and method for producing the same) relates to 100% by weight of bean ground product, 15 to 75 parts by weight of gluten, 7 to 38 parts by weight of nuts, 40 to 40 parts by weight of vegetable, 5 to 30 parts by weight of vegetable, 0.1 to 2.5 parts by weight of TG and 100 to 200 parts by weight of broth. The soybean meat processed food is a soybean meat processed food which is at least one selected from the group consisting of soybean meat, bean sausage, bean ham, beanburger patty, soy bean curd, bean bulgogi, and soybean steak. Korean Patent Registration No. 10-1397794 (a method of producing soybean meat having improved palatability and antioxidative activity) comprises the steps of i) adding water to soybeans in an amount of 2 to 3 times and immersing the soybean at 20 ° C to 30 ° C for 8 to 16 hours; ii) removing water and increasing at 100 ° C for 1-2 hours; iii) drying the expanded soybeans at 50 DEG C to 60 DEG C for 2 to 3 days; iv) pulverizing the dried beans into a powder; And v) mixing powdered soybeans with a raw material, adding gravy, kneading and aging the mixture; (vi) molding the Congo machine in a mold, and / or (vii) step of adding. Korean Patent Publication No. 10-2017-0055356 (soybean meat having an increased content of conjugated linoleic acid and isoflavone, and a method for producing the same) is characterized in that (a) fermenting a fermented soybean mushroom culture broth to a roasted soybean, Preparing; (b) 47.5-57.5% by weight of fermented soybean mash mycelia powder, 8-16% by weight of mung bean powder, 2-6% by weight of starch, 3-7% by weight of gluten, 0.1-0.5% by weight of transglutaminase, 2.0% by weight and 20 to 35% by weight of the material; (c) mixing and kneading the broth with the solid material; And (d) aging the batter at 4-8 [deg.] C for 4 to 6 hours; (e) a step of molding the congo group in any one of the molds composed of hot bar, patty, ham, bulgogi, steak, and sausage, and further comprising a step of producing soybean meats with increased content of conjugated linoleic acid and isoflavones to be. Korean Patent Registration No. 10-1659655 (low-gluten-containing soybean meat and method for producing the same) is characterized in that it comprises: i) 30-52.9% by weight of soybean powder, 9-15% by weight of soybean powder, 8-16% by weight of mung bean powder, 0.1 to 1.0% by weight of gluten, 5 to 9% by weight of gluten, and 25 to 39% by weight of a sub ingredient; Ii) kneading the solid material with the broth; (Iii) aging the kneaded product at 4 to 8 DEG C for 4 to 6 hours, (iv) molding the conglomerate in any one of a hot bar, a patty and a sausage, There is a method of making meat. Other prior arts related to processing products using soybeans are Korean Patent Publication No. 10-2014-0143315, Korean Patent Laid-Open No. 2001-0054290, and Ultra Low-Liquid Chromatography Regarding Manufacturing Method of Ham and Sausage- Korean Patent Laid-Open Publication No. 2004-0076548 for processing fine sausage sausage, Korean Patent Laid-Open Publication No. 2010-033718 for manufacturing soybean meat for cooked bread, a method for producing soybean sausage using anthocyanin extract and protein extracted from black soybean Patent Registration No. 10-0936514, and Korean Patent Registration No. 10-0878329 on a method for producing soybean sausage using tissue soybean protein, which have a technology for producing soybean protein using soybean protein. In addition, as a conventional technology related to fermented soybeans, a method of producing soybean fermented product by inoculating and cultivating lactic acid bacterium into the expanded soybean or grown germinated soybean (Japanese Patent Application Laid-Open No. 10-2016-0056489), a method of producing soybean microorganism (Japanese Unexamined Patent Application Publication No. 10-2013-0056020), a method of producing a fermented soybean sprout food by inoculating and cultivating bacillus, fungus or yeast in a fermented soybean sprout 10-2007-0113674) and the like are disclosed.
콩은 식물성 고단백질에 콜레스테롤이 없어 세계적으로 육가공품을 대체할 수 있는 새로운 식품소재로 활용되고 있다. 그러나 국내외적으로 콩고기를 상품화한 제품은 기능성이 결여되어 있다. 따라서 최근 건강 지향적 식품에 관심이 높은 소비자의 요구를 제품에 반영한 소비자 니즈를 충족시킬 수 있는 건강한 육류 대체품 개발이 필요하다. 국내에서 식물성 단백식품의 시장규모는 연간 약 50억원 정도로 소비가 크게 늘지 못하고 있는 실정이다.Soybean has no high cholesterol in vegetable high protein and is being used as a new food material that can replace meat products worldwide. However, products that have been commercialized for domestic and international congo are lacking in functionality. Therefore, it is necessary to develop healthy meats substitute products that can meet consumer needs that reflect consumers' demand for products that are highly interested in health - oriented foods. The market size of vegetable protein food in Korea is about 5 billion won per year.
본 발명은 종래의 콩고기의 문제점을 해결하는 데 있다. 따라서 콩고기에 기능성을 부여하기 위하여 버섯균사체와 복분자 및 착즙액을 첨가하여 항산화활성을 제공하는 버섯균사체 발효콩고기의 제조방법을 제공하는데 있다. The present invention is to solve the problems of conventional soybean meat. Therefore, it is an object of the present invention to provide a method for producing fermented soybean meat of mushroom mycelia, which provides an antioxidant activity by adding mushroom mycelium, bokbunja and juice to congo.
보다 상세하게는 버섯 균사체를 이용하여 버섯 발효콩을 만드는 단계와, 복분자로부터 복분자추출액을 얻는 단계와, 정제수에 복분자추출액, 발효콩, 전분, 양파, 버섯, 당근, 참깨, 견과류 및 착즙액을 넣고 믹서로 분쇄한 다음, 부재료와 양념을 넣고 반죽기에서 반죽한 반죽물을 냉장 숙성시켜 콩고기를 만드는 단계와, 상기의 콩고기를 핫바, 패티, 햄, 불고기, 스테이크 및 소시지로 구성되는 어느 하나의 성형 틀에서 성형 및 증숙하는 단계로 구성되는 것을 특징으로 하는 발효콩고기의 제조방법으로 구성된다. More particularly, the present invention relates to a method for producing a mushroom fermented soybean using mushroom mycelium, a step of obtaining a bokbunja extract from a bokbunja, a step of adding bokbunja extract, fermented soybean, starch, onion, mushroom, carrot, sesame, A step of crushing the mixture with a mixer and then adding the ingredients and the seasoning to make a congee by aging the kneaded product dough kneaded in a kneader; and a step of preparing the congee by mixing the congee with any one of the molds consisting of hotba, patty, ham, bulgogi, steak and sausage And a step of forming and cooking the fermented soybean meat.
본 발명의 콩고기 가공식품은 콩고기, 콩소시지, 콩햄, 콩버거패티, 콩까스, 콩불고기 및 콩스테이크 군 중에서 어느 하나 이상일 수 있다.The soybean meat processed food of the present invention may be at least one of soybean meat, bean sausage, bean ham, beanburger patty, bean curd, bean bulgogi, and bean steak.
본 발명의 복분자를 첨가한 기능성 버섯 발효콩고기는 다양한 형태로 성형될 수 있으며, 기능성 및 기호성이 우수한 콩고기를 제공할 수 있다.The functional mushroom fermented soybean curd with the addition of the brambles of the present invention can be shaped into various forms and can provide a soybean curd refiner having excellent functionality and palatability.
도 1은 본 발명의 콩고기의 제조방법을 나타낸다.
도 2는 본 발명의 콩고기 제품의 항산화활성을 나타낸 것이다.Fig. 1 shows a method for producing soybean meat of the present invention.
Fig. 2 shows antioxidant activity of soybean meat products of the present invention.
1) 버섯 균사체를 이용하여 발효콩 분말을 만드는 단계1) Making fermented soybean powder using mushroom mycelium
유기농 콩(가락시장에서 구입) 5kg에 22~28℃의 정제수 10~15 리터를 가하여 12~24시간 동안 침지시킨 다음, 침지된 콩을 건져내고 물기를 제거한 후 증자기에 넣고 95~105 ℃에서 1~2시간 동안 콩을 삶는다. 삶은 콩에 버섯균사체 배양액을 10%를 접종하여 발효시킨 후, 버섯균사체 발효콩을 35~45℃ 에서 12~24시간 동안 건조시킨 다음 건조된 콩을 20~100 mesh 크기로 분쇄하여 발효콩분말 4kg을 얻었다. 10 ~ 15 liters of purified water at 22 ~ 28 ℃ is added to 5kg of organic soybeans (purchased from Garak Market) and soaked for 12 ~ 24 hours. Then the soaked soybeans are removed and the water is removed. Boil the beans for 2 hours. The fermented soybeans of mushroom mycelium were dried at 35 ~ 45 ℃ for 12 ~ 24 hours, and the dried soybeans were pulverized into 20 ~ 100 mesh size and 4 kg of fermented soybean powder ≪ / RTI >
버섯균사체는 액체배지(5%), 25℃, 120rpm에서 균사체의 계대 배양은 효모 펩톤아가(YPD agar, Difico, MI, U.S.A)와 포테이토 덱스트로스 아가(PDA agar, Difico, MI, U.S.A)를 사용하였다. 버섯 균사체를 평판 배지를 사용하여 배양한 후, 4℃의 냉장고에 보관하면서 1개월 이내에 사용하였다. 콩 액체배지(5%)에서 빠른 성장(7일 이내)을 보인 기계충 버섯균사체, 느타리 버섯균사체를 선별하여, 25℃, 120rpm에서 1차 활성화 시킨 후, 이 균사체액을 농도별(1%, 3%, 5%, 10%)(v/v)로 다시 콩 액체배지(5%)에 접종하여, 균사체량과 β-glucosidase 활성을 체크하여, 콩 액체배지(5%)에서의 버섯균사체 액체종균은 25℃, 120rpm에서 5~7일 2차 활성화시킨 버섯균사체를 10% 접종하였다.Mushroom mycelium was cultured in liquid medium (5%) at 25 ℃ and 120 rpm using yeast peptone agar (YPD agar, Difico, MI, USA) and potato dextrose agar (PDA agar, Difico, MI, USA) Respectively. The mushroom mycelium was cultured in a plate medium and stored in a refrigerator at 4 ° C for 1 month. The mycelium and the mycelium of the mushroom and the mushroom which showed rapid growth (within 7 days) in the soybean liquid medium (5%) were selected and activated first at 25 ° C and 120 rpm. (5%) in a soybean liquid medium (3%, 5%, 10%) (v / v) to check mycelium amount and β-glucosidase activity, The seedlings were inoculated with mushroom mycelium which had been activated at 25 ° C. and 120 rpm for 5 to 7 days at 10%.
버섯 균사체 중에서 표고버섯(Lentinula edodes) 균사체는 KCTC 6734, 차가버섯(Inonolus obiquus) 균사체는 KCTC 26147, 송이버섯(Tricholoma matsutake) 균사체는 KCTC 26249, 느타리버섯(Pleurotus ostreatus) 균사체는 KCTC 16855, 상황버섯(Phellinus linteus) 균사체는 KCTC 6190, 영지버섯(Ganoderma lucodum) 균사체는 KCTC 6283, 기계충 버섯(Irpex lacteus) 균사체는 전북 김제 소재 종균 분양소 중에서 분양받아서 사용할 수 있다. 그밖에 균사체는 시중에서 구입(표고버섯균사체 AHCC(2008-78호)하여 사용할 수 있다. Among the mushroom mycelia, KCTC 6734, KCTC 26147, KCTC 26147, Tricholoma matsutake, KCTC 26249, Pleurotus ostreatus mycelium, KCTC 16855 and mushroom, respectively, were found to be mycelia of Lentinula edodes, KCTC 6734, Inonolus obiquus, Phellinus linteus mycelium KCTC 6190, Ganoderma lucodum mycelium KCTC 6283, and Irpex lacteus mycelium can be purchased from the seed pots in Gimje, Jeonbuk. In addition, mycelium is commercially available (available from mushroom mycelium AHCC (2008-78)).
2) 복분자추출액을 얻는 단계2) Step of Obtaining Bokbunja Extract
고창에서 수확한 복분자 열매 5kg을 구입하여 으깨기한 다음, 과육과 즙을 얻은 후, 정제수를 8~12 리터를 혼합하여 50~60℃에서 12 내지 24시간 동안 열수로 복분자추출액 10 리터를 얻었다. 반죽에 사용하는 정제수를 대신에 희석된 복분자 추출액을 첨가하여 반죽하면 물 사용량을 절감할 수 있다. After obtaining 5 kg of bokbunja fruit harvested from Gochang, they were mixed with 8 to 12 liters of purified water, and 10 liters of bokbunja extract was obtained by hot water for 12 to 24 hours at 50 to 60 ° C. Instead of the purified water used in the dough, the diluted brambone extract solution is added and kneaded to reduce the amount of water used.
3) 원부재료의 분쇄, 반죽 및 콩고기 제조 단계3) Crushing, dough and bean meat production of raw materials
정제수 적당량, 복분자추출액 8~12 리터, 발효콩분말 3.5~4.5kg, 전분 50~100g, 양파 10~20g, 파 10~20g, 견과류 10~20g, 버섯 10~20g, 당근 5~10g, 참깨 5~10g을 넣고 믹서로 분쇄한 다음, 부재료로서 글루텐 150~200g, 빵가루 10~20g, 트란스글루타미나제 1.5~5g 또는 가수분해단백(HVP) 50~100g을 추가로 넣고 반죽기에서 반죽한다. 상기에서 전분은 도토리전분, 타피오카전분, 옥수수전분 또는 고구마전분 중에서 어느 하나 또는 둘 이상을 사용할 수 있다. 상기에서 버섯은 양송이버섯, 표고버섯, 느타리버섯, 능이버섯 또는 싸리버섯 중에서 어느 하나 또는 둘 이상을 사용할 수 있다. 상기에서 견과류는 땅콩, 아몬드, 호두, 해바라기씨, 밤, 은행, 잣, 캐슈넛, 피스타치오, 피칸 및 호박씨 중에서 어느 하나 또는 둘 이상을 사용할 수 있다.Purified water suitable amount, 8 ~ 12 liters of bokbunja extract, 3.5 ~ 4.5kg of fermented soybean powder, 50 ~ 100g of starch, 10 ~ 20g of onion, 10 ~ 20g of pork, 10 ~ 20g of nuts, 10 ~ 20g of mushroom, And 10 g of bread, 10 to 20 g of bread flour, 1.5 to 5 g of transglutaminase, or 50 to 100 g of hydrolyzed protein (HVP) are further added as a sub ingredient and kneaded in a kneader. The starch may be any one or more of acorn starch, tapioca starch, corn starch, and sweet potato starch. The mushroom may use one or more of mushroom mushroom, shiitake mushroom, oyster mushroom, mushroom mushroom or mushroom mushroom. The nuts may be any one or more of peanut, almond, walnut, sunflower seed, chestnut, bank, pine nut, cashew nut, pistachio, pecan and pumpkin seeds.
상기 단계에서 양념으로서 설탕 50~100g, 소금 5~10g, 간장 50~100ml, 마늘가루 50~100g, 후추가루 50~100g, 또는 육수 0.5~1리터를 추가로 혼합하여 반죽한다. 상기의 반죽물을 냉장고에서 숙성시켜 보관한다. 상기에서 착즙액은 과일즙, 야채즙, 해조류즙 또는 육즙 중 어느 하나 또는 둘 이상이 포함될 수 있다.In the above step, 50 to 100 g of sugar, 5 to 10 g of salt, 50 to 100 ml of soy sauce, 50 to 100 g of garlic powder, 50 to 100 g of black pepper powder or 0.5 to 1 liter of broth are further mixed and kneaded. The kneaded product is aged in a refrigerator and stored. The juice solution may include one or more of fruit juice, vegetable juice, seaweed juice or juice.
과일즙은 배, 사과, 석류, 포도, 무화과, 레몬, 복분자, 아로니아, 블루배리 중에서 착즙한 어느 하나 또는 둘 이상이 포함될 수 있다. 야채즙은 토마토, 양배추, 당근, 울금, 무, 브로콜리, 시금치 또는 당근 중에서 착즙한 어느 하나 또는 둘 이상이 포함될 수 있다. 해조류즙은 갈조류(톳, 미역, 다시마, 대황, 모자반 등), 홍조류(우뭇가사리, 김, 카라니긴 등), 녹조류(파래, 청각, 청태 등)에서 착즙 또는 추출한 어느 하나가 포함될 수 있다.The fruit juice may include one or two or more of juice in a pear, an apple, a pomegranate, a grape, an fig, a lemon, a bramble, an aronia, and a blue barley. The vegetable juice may include one or more of tomato, cabbage, carrot, radish, radish, broccoli, spinach or carrot juice. Seaweed juice can be either juice or extracted from brown algae (red sea bream, seaweed, kelp, rhubarb, mung bean, etc.), red algae (mugwort, kim, caranigin etc.) and green algae (parasites, hearing,
육즙은 쇠고기육수, 뼈육수, 참치육수, 게육수, 가재육수 중에서 착즙한 어느 하나 또는 둘 이상이 포함될 수 있다.The juice may include any one or two or more of the juice in beef broth, bone broth, tuna broth, crab broth, and home-grown broth.
상기의 콩반죽믈의 숙성은 콩 가공식품 반죽에 포함되어 있는 여러 가지 종류의 식품 성분 중의 수분을 고르게 분포시켜 주며, 상기 숙성의 조건은 10℃ 이하에서 10시간 이내에 미생물의 번식을 방지하면서 숙성시켜야 한다. The aging of the above-mentioned bean paste doughs evenly distributes the moisture among various kinds of food ingredients contained in the bean processed food dough and the aging condition is to aged while preventing the propagation of microorganisms within 10 hours at 10 ° C or less do.
4) 성형 및 증숙 단계4) Molding and steaming stage
상기의 콩고기 반죽을 다양한 형태의 성형 틀에서 핫바, 패티, 햄, 불고기, 스테이크 및 소시지 등으로 만들 수 있다. 성형틀에 콩고기 반죽을 넣고 성형한 다음 95∼105 ℃에서 25∼35분 동안 가열한 후 10℃ 이하로 냉각하여 콩고기 가공식품을 제조한다.The above-mentioned bean meat dough can be made into various forms of molds such as hot bar, patty, ham, bulgogi, steak and sausage. The bean meat dough is put into a molding frame, molded, and then heated at 95 to 105 ° C for 25 to 35 minutes and then cooled to 10 ° C or lower to produce processed soybean meat.
<실시예 1-4> ≪ Example 1-4 >
상기의 방법과 같이 버섯균사체 배양액 이용하여 발효콩 분말을 만든 다음에 미리 제조한 복분자추출액을 얻은 후, 이들 원부재료를 다음의 배합비와 같이하여 분쇄, 반죽 및 콩고기를 제조하였다.The fermented soybean powder was prepared by using the mushroom mycelia culture liquid as described above, and then the pre-prepared brambone extract was obtained. Then, these raw materials were pulverized, kneaded and congealed by the following mixing ratios.
상기에서 과일즙은 배즙이고, 야채즙은 무즙과 당근즙이고, 육즙은 쇠고기육수와 뼈육수이며, 해조즙은 다시마추출액이다.In the above, fruit juice is juice, vegetable juice is juice and carrot juice, juice is beef broth and bone marrow, and seaweed juice is seaweed extract.
<시험예 1>; 물성 측정≪ Test Example 1 > Property measurement
상기의 실시예 1 내지 4 및 대조군의 배합비와 같이 제조한 콩고기의 물성을 알아 보기 위하여 가로 세로 높이를 일정한 크기(5 ㎝ × 5 ㎝ × 1 ㎝)로 절단하여 물성분석기(Stable Micro System사, 제품명: TA.XTEpress)를 이용하여 측정한 물성 결과는 다음의 표 2에 나타냈다.In order to examine the physical properties of soybean meat prepared according to the mixing ratios of Examples 1 to 4 and the control group, the height and height of the soybean meat were cut to a certain size (5 cm x 5 cm x 1 cm) and analyzed using a physical property analyzer (Stable Micro System, ≪ tb > < SEP > product name: TA.XTEpress) is shown in Table 2 below.
상기 표 2에 나타난 바와 같이, 콩고기 각각의 물성을 측정한 결과 실시예 1 내지 실시예 4의 견고성이 대조군 보다 높게 나타났다. 또한, 실시예 1 내지 4의 저작성은 대조군 보다 낮았으며, 검성은 대조군의 콩고기가 실시예 1 내지 실시예 4 보다 약간 높거나 비슷한 수준을 나타냈다.As shown in Table 2, the physical properties of soybean meat were measured, and the firmness of Examples 1 to 4 was higher than that of the control. In addition, the chewing of Examples 1 to 4 was lower than that of the control group, and the gonorrhea showed a slightly higher or similar level than that of the Examples 1 to 4 in the control group.
<시험예 2>; 색도 측정≪ Test Example 2 > Chromaticity measurement
색도는 색차계(Minolta사 제품명: CM-3500d)를 이용하여 측정하였다. 상기에서 측정한 물성 결과는 다음의 표 3에 그 결과를 나타냈다.The chromaticity was measured using a color difference meter (product name: CM-3500d, Minolta). The results of physical properties measured above are shown in Table 3 below.
표 3에 나타난 바와 같이 실시예 1 내지 4 및 대조군 콩고기의 색도를 측정한 결과 밝기를 나타내는 L값은 대조군에 비하여 실시예 1 내지 4가 약간 우수하고, 적색도인 a 값 및 황색도인 b 값은 대조군과 모두 비슷한 수준이었다.As shown in Table 3, the chromaticities of Examples 1 to 4 and the control soybean meat were measured. As a result, the L value indicating the brightness was slightly better than that of the control group, and the a value and the b value Were similar to those of the control group.
<시험예 3>; 관능검사≪ Test Example 3 > Sensory test
콩고기의 기호성 평가를 위한 관능검사는 잘 훈련된 남녀 각각 30명(10대, 20대, 30대)을 대상으로 5점척도법(매우 좋다 5점, 좋다 4점, 보통이다 3점, 나쁘다 2점, 매우 나쁘다 1점)으로 하여 평가하였다. 관능평가의 결과는 다음의 표 4에 나타냈다.The sensory evaluation for the palatability of soybean meat was conducted in 30 well - trained men and women (10, 20, and 30 years old). The 5 point scale method (very good 5 points, good 4 points, Point, very bad 1 point). The results of the sensory evaluation are shown in Table 4 below.
표 4에서 나타난 바와 같이대조군에 비하여 실시예 1 내지 실시예 4에서 맛, 색 및 전체적인 기호도에서 높은 평가를 받은 것을 확인하였다.As shown in Table 4, it was confirmed that in Examples 1 to 4, the taste, color and overall acceptability were higher than those of the control group.
<시험예 4>; 콩고기의 총 페놀릭스 및 총 플라보노이드 함량≪ Test Example 4 > Total phenolics and total flavonoid content of soybean meat
각각의 시료에 대해서 총 페놀릭스 Folin-Ciocalteu 법으로 측정하였다. 준비된 각각의 시료를 0.45㎛ 필터로 여과한 액을 100배 희석한 후 0.5 ml을 시험관에 분주하고 25% Na2CO3 용액 0.5 ml을 첨가하여 3분간 정치시켰다. 다시 2N-Folin-Ciocalteu 페놀 시약 0.25 ml 첨가하여 혼합한 다음, 상온에서 1 시간 동안 정치시켜 발색시켰다. 발색된 청색을 분광광도계(Spectronic 2D)를 이용하여 750 nm에서 흡광도를 측정하였다. 이때 총 페놀릭스 함량은 갈산(gallic acid)을 이용하여 작성한 표준곡선으로부터 함량을 구하였다. 갈산을 이용한 표준곡선은 갈산의 최종농도가 0, 25, 50, 100 mg/l이 되도록 하여 위와 같은 방법으로 750 nm에서 흡광도를 측정하여 그 결과를 도 2에 나타냈다. Each sample was measured by the total phenolics Folin-Ciocalteu method. Each of the prepared samples was diluted 100 times with a 0.45 μm filter and 0.5 ml was added to a test tube. 0.5 ml of 25% Na 2 CO 3 solution was added and the mixture was allowed to stand for 3 minutes. Then, 0.25 ml of 2N-Folin-Ciocalteu phenol reagent was added to the mixture, and the mixture was allowed to stand at room temperature for 1 hour to develop color. The color developed blue was measured for absorbance at 750 nm using a spectrophotometer (Spectronic 2D). The content of total phenolics was determined from the standard curves prepared using gallic acid. The absorbance of the standard curve using galactan was measured at 750 nm in the same manner as above, so that the final concentration of gallic acid was 0, 25, 50, and 100 mg / l. The results are shown in FIG.
도 2에 나타난 바와 같이, 대조군의 콩고기에 비하여 본 발명에 따른 콩고기의 총 페놀릭스 함량이 높았다. 총 플라보노이드 함량은 준비된 각각의 시료를 0.45㎛ 필터로 여과한 액을 50배 희석한 후 0.5 ml을 시험관에 분주하고 디에틸렌 글리콜 1.0 ml, 1N-NaOH 0.01 ml를 가한 후, 37℃ 항온 수조에서 1시간 동안 방치한 후 분광광도계(Spectronic 2D)를 이용하여 420 nm에서 흡광도를 측정하였고 그 결과를 도 2에 나타냈다. 이때 총 플라보노이드 함량은 루틴(Rutin)을 이용하여 작성한 표준곡선으로부터 함량을 구하였다. 도 2에 나타난 바와 같이, 대조예 콩고기에 비하여 본 발명에 따른 콩고기의 총 플라보노이드 함량이 훨씬 높았다.As shown in Fig. 2, the total phenolic content of soybean meat according to the present invention was higher than that of the control soybean group. The total flavonoid content of each sample was diluted 50-fold with a 0.45-μm filter, and 0.5 ml of diethylene glycol and 1.0 ml of 1N-NaOH were added to the test tube. The absorbance was measured at 420 nm using a spectrophotometer (Spectronic 2D), and the results are shown in Fig. The content of total flavonoid was determined from the standard curve prepared using Rutin. As shown in FIG. 2, the total flavonoid content of soybean meat according to the present invention was much higher than that of the control soybean congo.
<시험예 5>; 콩고기의 항산화 활성 검정≪ Test Example 5 > Antioxidant activity test of soybean meat
준비된 콩고기의 추출물 시료에 대해서 DPPH 라디칼 소거활성, ABTs 라디칼 소거활성 및 환원력(FRAP) 분석을 통하여 항산화 활성을 검정하였다. DPPH(1,1-diphenyl-2-picrylhydrazyl, Sigma D9132, FW 393.4, C18H12N5O6)는 매우 안정한 라디칼로서 517nm에서 특정 흡광도를 나타내는 보라색 화합물이다. DPPH 라디칼 소거활성을 측정하여 항산화성을 확인하였다. 구체적으로는 두 가지 콩고기의 농도별 추출물 시료 각각 0.2 ml에, 에탄올로서 1.5 × 10-4 M 농도가 되게 한 DPPH 용액 0.8 ml씩을 보텍스(vortex)로 균일하게 혼합한 다음 실온에서 30분간 방치한 후 525 nm에서 흡광도(O.D.)를 측정하였다. 음성 대조구 실험은 시료 대신에 증류수를 0.2 ml를 취하여 실험하였다. DPPH 라디칼 소거활성은 아래와 같은 식으로 계산하여 백분율(%)로 표시하였으며, 그 결과를 표 5 및 도 2에 나타냈다.Antioxidant activity of DPPH radical scavenging activity, ABTs radical scavenging activity and reducing power (FRAP) analysis were examined for the extract of soybean meat. DPPH (1,1-diphenyl-2-picrylhydrazyl, Sigma D9132, FW 393.4, C18H12N5O6) is a very stable radical and a violet compound exhibiting a specific absorbance at 517 nm. DPPH radical scavenging activity was measured to confirm antioxidant activity. Specifically, 0.8 ml of a DPPH solution having a concentration of 1.5 × 10 -4 M as ethanol was uniformly mixed in a vortex to each 0.2 ml of the extract samples of the two soybean concentrates, and the mixture was allowed to stand at room temperature for 30 minutes The absorbance (OD) was measured at 525 nm. In the negative control experiment, 0.2 ml of distilled water was used instead of the sample. The DPPH radical scavenging activity was expressed as a percentage (%) by the following formula, and the results are shown in Table 5 and Fig.
DPPH 라디칼 소거활성(%) = [1-(음성대조구 흡광도 ÷ 실험구 흡광도)] × 100DPPH radical scavenging activity (%) = [1- (negative control absorbance ÷ experimental spherical absorbance)] × 100
표 5 및 도 2에 나타난 바와 같이, 대조군 콩고기에 비하여, 본 발명에 따른 콩고기의 DPPH 라디칼 소거활성이 높았다. 즉 본 발명의 실시예에 의해 DPPH 라디칼 소거활성이 증가함을 확인할 수 있다. As shown in Table 5 and FIG. 2, DPPH radical scavenging activity of soybean meat according to the present invention was higher than that of the control Konggonggi. That is, it can be confirmed that the DPPH radical scavenging activity is increased by the embodiment of the present invention.
ABTs 라디칼 소거활성은 2-아지노-비스의 색을 띤 라디칼의 감소 정도에 따라 항산화능을 검사하는 방법이다. 즉 이 방법은 시료와 표준물질(Trolox, 6-hydroxy -2,5,7,8-tetramethylchroman-2-carboxylic acid)의 값과 비교하여 항산화능을 측정하는 방법으로서, 시료의 항산화력에 의해 ABTS 양이온(ABTS)이 소거되어 청록색으로 탈색되는데, 이때 ABTS 양이온(ABTS)의 제거 정도를 흡광도를 측정함으로서 알 수 있고, 탈색반응이 1분 내에 종료되므로 짧은 시간에 측정이 가능하다. 구체적으로 7 mM ABTS 시약 (Sigma 1888) 5 ml과 140 mM K2S2O8 (FW 270.3, Sigma 9392) 5 ml을 섞어 어두운 곳에 14 ~ 16시간 방치시킨 후, 이를 무수 메탄올과 약 1 : 88 비율로 섞어 732 nm에서 대조구의 흡광도 값이 0.70 ± 0.02가 되도록 조절한 ABTS 용액을 사용하였다. 각각의 시료 0.1 ml과 ABTS 용액 0.9 ml를 혼합하여 30초간 진탕한 후 3분간 반응시키고 732 nm에서 흡광도를 측정하였다. 음성 대조구 실험은 시료 대신에 증류수로 실험하였다. ABTS 라디칼 소거활성은 아래와 같은 식으로 계산하여 백분율(%)로 표시하였으며, 그 결과를 표 5 및 도 2에 나타냈다.The ABTs radical scavenging activity is a method for examining the antioxidant ability of 2-azino-bis according to the degree of reduction of colored radicals. In other words, this method is a method to measure the antioxidant ability by comparing with the value of sample and reference material (Trolox, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) The cations (ABTS) are cleared and decolorized into cyan. At this time, the degree of ABTS cations (ABTS) can be detected by measuring the absorbance, and the decoloring reaction is completed within 1 minute. Specifically, 5 ml of 7 mM ABTS reagent (Sigma 1888) and 5 ml of 140 mM K2S2O8 (FW 270.3, Sigma 9392) were mixed and allowed to stand in the dark for 14 to 16 hours. The mixture was mixed with methanol in an amount of about 1:88, In which the absorbance of the control was adjusted to 0.70 ± 0.02. 0.1 ml of each sample and 0.9 ml of ABTS solution were mixed, shaken for 30 seconds, reacted for 3 minutes and absorbance was measured at 732 nm. Negative control experiments were performed with distilled water instead of samples. The ABTS radical scavenging activity was expressed as a percentage (%) by the following formula, and the results are shown in Table 5 and FIG.
ABTS 라디칼 소거활성(%) = [0100] [1-(음성대조구 흡광도 ÷ 실험구 흡광도)] × 100 ABTS radical scavenging activity (%) = [1- (negative control absorbance / experiment absorbance)] × 100
표 5 및 도 2에 나타난 바와 같이, 대조예 콩고기에 비하여, 본 발명에 따른 콩고기의 ABTS 라디칼 소거활성이 높았다. 본 발명의 제조과정에 의해 ABTS 라디칼 소거활성이 증가함을 확인할 수 있다. As shown in Table 5 and FIG. 2, the ABTS radical scavenging activity of soybean meat according to the present invention was higher than that of the control soybean concentrate. It can be confirmed that the ABTS radical scavenging activity is increased by the manufacturing process of the present invention.
환원력 분석은 FRAP 분석을 기초로 한 Liu 등의 방법을 응용하여 측정하였다. 구체적으로 FRAP 환원력 분석에서 반응액으로는 300mM 아세테이트 완충액(pH 3.6), 40 mM 염산에 녹인 10 mM 2,4,6-트리피리딜-s-트리아진(TPTZ, T1253, C18H12N6, MW312.33) 및 20mM FeCl3(F7134, MW 162.20)를 준비하였으며, 아세테이트 완충액, TPTZ 용액 및 FeCl3 용액을 10 : 1 : 1 (v/v/v)로 혼합하여 37℃ 에서 15분 간 예비반응을 시켜두었다. 참고예에 따른 농도별 시료 각각 0.1 ml과 예비반응된 FRAP 시약 0.9 ml을 96-웰 플레이트에 분주한 후 15분간 반응시키고 마이크로플레이트 리더(Biorad 3055, Sweden)를 사용하여 590 nm에서 흡광도를 측정하여 그 결과를 표 5 및 도 2에 나타냈다. 표 5 및 도 2에 나타난 바와 같이, 대조군 콩고기에 비하여, 본 발명에 따른 콩고기의 ABTS 라디칼 소거활성이 높았다. 본 발명의 제조과정에 의해 ABTS 라디칼 소거활성이 증가함을 확인할 수 있다. 상기의 결과들로부터 본 발명에 따라 제조된 콩고기는 우수한 항산화 활성을 나타냄을 알 수 있다.Reducing power was measured by using Liu et al. Based on FRAP analysis. Specifically, in the FRAP reduction assay, 10 mM 2,4,6-tripyridyl-s-triazine (TPTZ, T1253, C18H12N6, MW312.33) dissolved in 40 mM acetate buffer (pH 3.6) And 20 mM FeCl 3 (F7134, MW 162.20) were prepared, and the acetate buffer, the TPTZ solution and the FeCl 3 solution were mixed at a ratio of 10: 1: 1 (v / v / v) . 0.1 ml of each sample according to the reference example and 0.9 ml of the pre-reacted FRAP reagent were dispensed into a 96-well plate, reacted for 15 minutes, and the absorbance was measured at 590 nm using a microplate reader (Biorad 3055, Sweden) The results are shown in Table 5 and Fig. As shown in Table 5 and FIG. 2, the ABTS radical scavenging activity of soybean meat according to the present invention was higher than that of the control soybean congee. It can be confirmed that the ABTS radical scavenging activity is increased by the manufacturing process of the present invention. From the above results, it can be seen that the Congo group prepared according to the present invention shows excellent antioxidative activity.
구 분
division
총폴리페놀함량 (mg/g)
Total polyphenol content (mg / g)
총플라보노이드함량(mg/g)
Total flavonoid content (mg / g)
DPPH라디컬소거활성(%)
DPPH radical scavenging activity (%)
ABTs라디컬소거활성(%)
ABTs radical scavenging activity (%)
FRAP 환원력(OD)
FRAP reducing power (OD)
본 발명의 복분자를 첨가한 기능성 버섯 발효콩고기는 다양한 형태로 성형될 수 있으며, 기능성 및 기호성이 우수한 콩고기를 제공할 수 있다.The functional mushroom fermented soybean curd with the addition of the brambles of the present invention can be shaped into various forms and can provide a soybean curd refiner having excellent functionality and palatability.
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KR101514534B1 (en) | 2013-07-31 | 2015-05-21 | 대한민국(농촌진흥청장) | Soybean-meat processed food and manufacturing method thereof |
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