KR101631225B1 - Antimicrobial agent composition for water tissue, water tissue comprising the same and preparation method thereof - Google Patents

Abstract

The present invention relates to a antimicrobial composition for a wet tissue, a wet tissue including the same, and a method for producing the same.
The antimicrobial composition for a wet tissue according to the present invention, the wet tissue including the wet tissue, and the method for producing the same, while maintaining excellent antibacterial activity, stably and firmly express an appropriate pH of the wet tissue. In particular, the pH of the entire wet tissue is constantly expressed in a pH range of 4-6 at an appropriate level, regardless of whether the initial pH of the nonwoven fabric used as the raw material of the wet tissue is high or low.

Description

TECHNICAL FIELD The present invention relates to an antimicrobial composition for a wet tissue, a wet tissue including the same, and an antimicrobial agent composition for the same,

The present invention relates to a antimicrobial composition for a wet tissue, a wet tissue including the same, and a method for producing the same.

Microorganisms are useful depending on the application, but they cause direct and indirect losses for nutrient and water-containing products.

In particular, products such as wet tissues are not only deteriorated by microorganisms but also frequently washed with water for a long time in a state in which they are directly applied to the skin. Therefore, when the preservative system for preventing microbial contamination is mistaken, Or may cause illness. Therefore, these wipes have adopted appropriate antibacterial agents to prevent contamination by microorganisms.

According to reports of research on the cosmetic ingredients of the ENVIRONMENTAL WORKING GROUP (EWG), a US environmental movement group, it has been reported that food additives can be used as cosmetic ingredients. However, studies on the possibility of using these food additives as antimicrobial agents for wet tissues are in short supply.

On the other hand, the nonwoven fabric has a planar structure formed by joining various fibers such as natural, chemical, glass, metal, and air according to the characteristics of each other to form a sheet web and mechanically or physically combining the fibers. Nonwoven fabrics are called nonwoven fabrics. Woven fabrics for making wet wipes are various, but generally used fabrics are controlled by adjusting the ratio of rayon to polyester to improve the feel or control the physical properties. Among the raw materials of the nonwoven fabrics, rayon is used as an eco-friendly material. The fibers are made of wood, and the fibers are regenerated with cellulose by chemical treatment of strong acid such as sulfuric acid, In the post-process, caustic soda or the like is used to improve the physical properties. However, treatment of these materials may affect the pH of the nonwoven fabric, or may affect the pH by the residue, which may add to the pH of the final product, It works as a problem. In addition to these problems, there is a problem that the pH of the wet tissue is changed due to the interaction between the antimicrobial agent and the nonwoven fabric contained in the wet tissue, thus making it difficult to develop a stable and stable pH.

Although there are no laws or regulations stipulated in relation to the proper pH of the wet tissue, it is known that the optimum pH is preferably set at about 4-6 in consideration of prevention of trouble with the skin and the like. However, due to the pH problem of the nonwoven fabric and the deterioration of pH due to the treatment of additives such as antimicrobial agents, the pH of the wet tissue, which is the final product, is difficult to express the proper pH, Lt; / RTI >

Japanese Patent Application No. 10-1218829 (Patent Document 1) discloses a prior art document related to the present invention. In Patent Document 1, a wet wipes are used, A method for producing a used wet tissue has been disclosed, but no disclosure or suggestion has been made with respect to a wet tissue having a stable and stable pH at an appropriate pH.

Patent Document 1. Korean Patent No. 10-1218829

Disclosure of Invention Technical Problem [8] The present invention has been made to solve the above problems, and an object of the present invention is to provide a antimicrobial composition for a wet tissue in which an appropriate pH of the wet tissue is stably expressed while exhibiting excellent antibacterial activity and a wet tissue containing the same. It is an object of the present invention to provide a composition for a wet tissue using only a food additive having a low possibility of harming the human body while maintaining the pH of the wet tissue constant regardless of whether the initial pH of the nonwoven fabric is high or low.

According to an aspect of the present invention, there is provided a composition for a wet tissue, comprising water, levulinic acid, cetylpyridinium chloride, and sodium citrate.

A method of manufacturing a wet tissue according to another aspect of the present invention includes:

1) mixing cetylpyridinium chloride with water, levulinic acid and sodium citrate mixture to obtain a wettable antimicrobial composition; And

2) spraying the composition onto one or a plurality of nonwoven fabrics stacked thereon, followed by squeezing, or dipping one or more nonwoven fabrics stacked in the composition and pressing them;

.

A wet tissue according to another aspect of the present invention includes water, levulinic acid, cetylpyridinium chloride, and sodium citrate, and includes a nonwoven fabric having a pH of 4-6 on a 0.1-0.8% aqueous solution.

The antimicrobial composition for a wet tissue according to the present invention, the wet tissue including the wet tissue, and the method for producing the same, while maintaining excellent antibacterial activity, stably and firmly express an appropriate pH of the wet tissue. In particular, the pH of the entire wet tissue is constantly expressed in a pH range of 4-6 at an appropriate level, regardless of whether the initial pH of the nonwoven fabric used as the raw material of the wet tissue is high or low.

Accordingly, the present inventors have made intensive efforts to develop a composition for a wet tissue in which an appropriate pH of the wet tissue is stably expressed even when applied to a nonwoven fabric while maintaining an excellent antibacterial activity even when applied to a wet tissue. As a result, the antibacterial composition for a wet tissue, A wet tissue containing the same, and a method for producing the same, thereby completing the present invention.

Generally, the wet tissue is manufactured using a nonwoven fabric as a main material. In order to prevent the contamination of the wet tissue from various microorganisms, the nonwoven fabric is treated with antimicrobial agents and preservatives. In this case, . Thus, most of the wet tissues do not cause troubles on the skin and are not suitable for the pH range of 4-6 which is not harmful to the human body. In particular, the non-woven fabric has a pH inherently possessed by each of the non-woven fabrics, which is not a pH suitable for use as a wet tissue. Even if the pH of the non-woven fabric originally possesses a proper pH, the pH of the nonwoven fabric originally possessed by the antimicrobial agent or other additives changes, . ≪ / RTI >

Specifically, the antimicrobial composition for a wet tissue according to the present invention comprises water, levulinic acid, cetylpyridinium chloride and sodium citrate.

The antimicrobial composition for a wet tissue according to the present invention can maintain an excellent antibacterial activity by treating the antimicrobial composition for a wet tissue with a nonwoven fabric used in the production of a wet tissue, regardless of the initial pH of the nonwoven fabric originally possessed by the nonwoven fabric, It is possible to achieve the desired pH constantly and stably.

The levulinic acid is obtained as a white crystalline material by heating fructose, inulin, starch and the like together with dilute hydrochloric acid and sulfuric acid, and can also be obtained as a natural extract in cornstarch. These levulinic acids are also used to make nylon, synthetic rubber, plastics, and medicines. The levulinic acid is in a solid state at room temperature, starts to melt at about 55 ° C or higher, and can be dissolved in water or an organic solvent.

On the other hand, levulinic acid can be used as a food flavoring agent and sodium citrate can be used as a beverage additive according to the report of the Environmental Working Group (EWG) of the US Environmental Action Group.

In addition, the levulinic acid has a pH of approximately 3 on 0.1-0.8% aqueous solution (hereinafter, the pH of the remaining substance or mixture is also a result of 0.1-0.8% aqueous solution), and the sodium citrate has a pH of approximately 7 . When such levulinic acid and sodium citrate are mixed together in water, the pH becomes approximately 5. When the non-woven fabric is treated with a non-woven fabric as the antimicrobial composition for wet tissues by mixing levulinic acid and sodium citrate, the non-woven fabric has an inherent pH (the initial pH originally possessed by the non-woven fabric is collected by measuring the solution squeezed from the non- The pH of 4-6 is expressed in the wet tissue. For example, even if the original initial pH of the nonwoven fabric becomes 2, when the mixture of water, levulinic acid, and sodium citrate is treated, the pH becomes 4-6, and even if the original initial pH of the nonwoven fabric becomes 8, When a mixture of acid and sodium citrate is processed, the pH will be 4-6. Also, when the pH is maintained at 4-6, the pH is constantly expressed at 4-6 even when a certain nonwoven fabric is used. The pH 4-6 is a range of pH values at which the wet tissue does not cause skin troubles when the wet tissue is used. In addition, by treating the mixture of water, levulinic acid and sodium citrate, the pH of the nonwoven fabric originally possessed by the nonwoven fabric was solved.

The preferable ratio of sodium citrate to levulinic acid for expressing the appropriate range of pH 4-6 is 1: 1-1: 4 (sodium citrate: levulinic acid), more preferably 1: 2 -1: 3. The appropriate pH of the wet tissue can be stably and stably expressed only when the content or ratio is satisfied. Particularly, when sodium citrate is mixed in the above-mentioned range of the content or ratio, the antibacterial activity to be achieved in the present invention is lowered, which is not preferable.

In addition, according to the present invention, not only the proper pH of the wet tissue is maintained at a constant value of 4-6, but also excellent antimicrobial activity is exhibited by mixing cetylpyridinium chloride, another component of the antimicrobial composition for wet tissues according to the present invention It is possible to keep it in an undamaged state.

The microorganism to which the antimicrobial activity exhibited by the antimicrobial agent for wet tissues according to the present invention is applied is not particularly limited, and preferably, the antimicrobial effect of Pseudomonas or Aspergillus niger . Pseudomonas aeruginosa is a microorganism belonging to the genus Pseudomonas, which produces fluorescein, fluorescein and pyocyanin, a green pigment, to produce green pus, causing cystitis, otitis media and empyema. In addition, the black fungus is a microorganism that helps human beings. However, when it is inhaled through the respiratory tract, it may cause pneumonia. If it occurs in a wet tissue, the product turns into black. Therefore, desirable.

The content of the antimicrobial composition for wet tissue is preferably 0.15-0.30 parts by weight of levulinic acid, 0.02-0.04 parts by weight of cetylpyridinium chloride, and 0.05-0.20 parts by weight of sodium citrate, based on 100 parts by weight of water. It is preferable that the appropriate pH value of the wet tissue is 4 to 6, and the antibacterial activity is also excellently exhibited.

On the other hand, although other organic acids other than the levulinic acid may be added to the preservative composition for wet tissues, when the organic acid other than levulinic acid is replaced with other organic acids, And it is difficult to simultaneously achieve a stable expression and an excellent antibacterial activity.

However, in the case where other organic acids other than levulinic acid are substituted, the effect achieved by the present invention is difficult to be manifested. However, when other organic acids such as lactic acid, malic acid, and formic acid are added to the levulinic acid, There is an effect of further increasing the effect of.

In particular, when lactic acid is further added to the antimicrobial composition for wet tissues according to the present invention, it becomes possible to more stably express the optimum pH, which is preferable. When the lactic acid is further added, the preferred content is 0.15-0.30 parts by weight of levulinic acid, 0.02-0.04 parts by weight of cetylpyridinium chloride, 0.05-0.20 parts by weight of sodium citrate, and 0.04-0.10 parts by weight of lactic acid based on 100 parts by weight of water The negative effects are more preferable because the effect of the present invention is further enhanced. On the other hand, it has been reported that the lactic acid can be used as a food acidifier such as Yakult according to EWG.

Although there is no particular limitation, the antibacterial composition for wet tissues according to the present invention may further include an emulsifier, a defoamer, a skin softening agent, and the like.

Thus, the antimicrobial composition for wet tissues according to the present invention not only exhibits excellent antibacterial activity but also is a biocidal composition for wet tissues utilizing only a food additive having a low possibility of causing harm to the human body, It is possible to maintain the antibacterial composition uniformly.

A method of manufacturing a wet tissue according to another aspect of the present invention includes:

1) mixing cetylpyridinium chloride with water, levulinic acid and sodium citrate mixture to obtain a wettable antimicrobial composition; And

2) spraying the composition onto one or a plurality of nonwoven fabrics stacked thereon, followed by squeezing, or dipping one or more nonwoven fabrics stacked in the composition and pressing them;

.

The order of mixing cetylpyridinium chloride with respect to the mixture of water, levulic acid and sodium citrate in the above step 1) is not particularly limited in the present invention.

The antibacterial composition for wet tissues is treated with the nonwoven fabric by the above step 2, and there is no particular limitation on the method of treatment. Preferably, the composition is sprayed onto one or a plurality of nonwoven fabrics, or one or a plurality The laminated nonwoven fabric is immersed in the composition and then dehydrated. At this time, the size of the nonwoven fabric or the thickness of the lamination layer is not particularly limited. There is no particular limitation on the method of spraying, squeezing, or dipping.

When the antimicrobial composition for wet tissue obtained by the above step 1) is treated to a nonwoven fabric as in step 2), the pH of the wetted wipe is adjusted to 4-6 in 0.1-0.8% aqueous solution irrespective of the original initial pH of the nonwoven fabric . The pH value of the wet tissue corresponds to the range of pH value of the wet tissue which does not cause skin troubles.

In addition, the wet tissue produced by such a manufacturing method is also excellent in antibacterial activity.

The preferable ratio of sodium citrate and levulinic acid for expressing the appropriate range of pH 4-6 is preferably 1: 1-1: 4 (sodium citrate: levulinic acid), more preferably 1 : 2-1: 3.

Further, if lactic acid is further added to the antimicrobial composition for a wet tissue according to the step 1), it becomes possible to more stably express an appropriate pH.

Although there is no particular limitation, the antimicrobial composition for wet tissues according to step 1) may further include an emulsifier, a defoamer, a skin softening agent, and the like.

A wet tissue according to another aspect of the present invention includes water, levulinic acid, cetylpyridinium chloride, and sodium citrate, and includes a nonwoven fabric having a pH of 4-6 on a 0.1-0.8% aqueous solution.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Example

Example  One

30 g of water, 60 g of water, 30 g of levulinic acid (Levinic acid ≥97%), 5 g of cetylpyridinium chloride (Merck, 'RonaCare CPC') and 10 g of sodium citrate (Sigma-aldrich) And the mixture was stirred for 1 hour to prepare the antimicrobial composition for wet tissues according to Example 1.

Example  2

10 g of lactic acid (Purac, HS-88) was further added to the antimicrobial composition for a wet tissue, and a antimicrobial composition for a wet tissue was prepared using the same method.

Example  3

To each of the antimicrobial compositions for wet tissues prepared according to Example 1 or 2, 49.5 g of secondary distilled water was added as a solvent, and 1 g of a perfume, 10 g of an emulsifier (Dipropylene Glycol) and 0.5 g of a defoamer (dimethicone) , And 4 g of Tetrasodium Pyrophosphate were added. And the mixture was stirred for 2 hours to prepare a final antimicrobial composition for a wet tissue.

Comparative Example

Comparative Example  One

A antibacterial composition for a wet tissue was prepared using the same method as in Example 1 except that levulinic acid was not added.

Comparative Example  2

A biocide composition for a wet tissue was prepared using the same method as in Example 1 except that sodium citrate was not added.

Comparative Example  3

The antibacterial composition for wet tissues was prepared in the same manner as in Example 1, except that the levulinic acid was not added and lactic acid (Purac, HS-88) was added in the same amount as levulinic acid.

Comparative Example  4

A biocide composition for a wet tissue was prepared in the same manner as in Example 1, except that the levulinic acid was not added and malic acid (Sigma-aldrich) was added in the same amount as levulinic acid.

Comparative Example  5

A biocide composition for a wet tissue was prepared in the same manner as in Example 1, except that the levulinic acid was not added and the same amount of formic acid (Sigma-aldrich) was added.

Comparative Example  6

A biocide composition for a wet tissue was prepared in the same manner as in Example 1, except that citric acid (Sigma-aldrich) was added in the same amount as levulinic acid without adding levulinic acid.

Comparative Example  7

A biocide composition for a wet tissue was prepared in the same manner as in Example 1, except that sodium citrate was not added and sodium hydroxide (Sigma-aldrich) was added in an amount equal to that of sodium citrate.

Comparative Example  8

A biocide composition for a wet tissue was prepared in the same manner as in Example 1 except that sodium citrate was not added and potassium hydroxide (Sigma-aldrich) was added in an amount the same as that of sodium citrate.

Comparative Example  9

To each of the antimicrobial agent compositions for wet tissues prepared according to Comparative Examples 1 to 8, 49.5 g of secondary distilled water was added as a solvent, and 1 g of a perfume, 10 g of an emulsifier (Dipropylene Glycol), 0.5 g of a defoamer (dimethicone) , And 4 g of Tetrasodium Pyrophosphate were added. And the mixture was stirred for 2 hours to prepare a final antimicrobial composition for a wet tissue.

Experimental Example

< Experimental Example  1: Synergistic effect on Pseudomonas aeruginosa>

An experiment was conducted to determine whether the mixture according to Example 1 exhibited a synergistic effect as a bactericidal composition for a wet tissue to achieve an excellent sterilizing power. However, aqueous mixtures of levulinic acid, sodium citrate and cetylpyridinium at different concentrations were prepared for this experiment in Example 1 and the effect of these mixtures on Pseudomonas aeruginosa ATCC 15442 was tested.

Specifically, the bactericidal composition for wet tissues mixed at the weight ratio shown in Table 1 below was diluted by a two fold dilution method using a Tryptic Soy Broth of Difco Co., After culturing the cultures containing the bactericides and microorganisms at 30 ° C. for 3 days, the turbidity of the lowest concentration that inhibited the growth was visually observed. This was then defined as the minimum inhibitory concentration (MIC) of each germicide or mixture thereof.

Table 1 below shows the concentrations of levulic acid (hereinafter referred to as LV), sodium citrate (Na-CA) and cetylpyridinium chloride (CPC) used. From this table, you can also check whether the growth of the microorganism is occurring (symbol "+") or not (symbol "-"). Table 1 shows not only growth but also minimum inhibition concentration (MIC).

The MIC values were 10,000 ppm when only LV was applied and 5,000 ppm when only CPC was employed.

Then the synergistic effect of the mixture as in Example 1 is shown in the form of calculated values using the calculation of the synergistic effect index shown in Table 2. &lt; tb &gt; &lt; TABLE &gt; The synergistic effect index is F.C. Kull et al., Applied Microbiology, vol. 9 (1961), p.538. Here, the synergistic effect index was calculated according to the following formula.

The synergy index SI = Qa / QA + Qb / QB

When applying this formula to a sanitizer system tested in the current situation, the variables in the formula have the following meanings.

Qa = LV concentration of the LV / CPC bactericidal mixture

QA = concentration of LV as the only component disinfectant

Qb = CPC concentration in the LV / CPC bactericidal mixture

QB = Concentration of CPC as the only component disinfectant

If the synergy index has a value of one or more, this means that antagonism is present. Assuming that the synergy index has a value of 1, this means that there is an effect of mixing the two substances. Assuming that the synergy index has a value of 1 or less, this means that there is a synergistic effect with the mixing of the two materials.

As can be seen from the above Table 2, when the proper concentration is maintained, the SI value of 1 or less is obtained. As a result, the bactericidal composition for wet tissues according to the present invention exhibits a synergistic effect of sterilizing power against P. aeruginosa . At this time, it was confirmed that when the synergy index of 0.35 and 0.45 was shown, the pseudomonas spp. It can also be seen from Table 2 that the best synergistic effect of the LV / CPC mixture with the lowest synergistic effect index (0.35) on P. aeruginosa is obtained as a mixture of 83 wt% LV and 17 wt% CPC. Experiments were conducted to confirm the titratable amounts of the mixture of 83 wt% LV and 17 wt% CPC (hereinafter, LCB mixture) and sodium citrate, and the results are shown in Table 3 below.

As a result of the experiment according to the above Table 3, it was confirmed that the titratable acidity of sodium citrate was 21% by weight, and as the amount of sodium citrate exceeded it, the antibacterial activity decreased.

< Experimental Example  2: Black mold  Synergistic effects on bacteria>

Similar to Experimental Example 1, an experiment was conducted to confirm whether the mixture of levulinic acid, sodium citrate and cetylpyridinium chloride exhibited a synergistic effect against microorganism Aspergillus niger ATCC 9642 with Example 1 Respectively.

The MIC values of the tested bactericidal compositions for wet tissues are shown in Table 4 below. The MIC values were 20,000 ppm when only LV was applied and 500 ppm when only CPC was employed.

Table 4 shows a synergistic effect when levulinic acid, sodium citrate and cetylpyridinium chloride were co-applied to black mold (Aspergillus niger ATCC 9642). The synergistic effect index was calculated in the same manner as in Experimental Example 1, and the results are shown in Table 5 below. At this time, the lowest synergistic effect index for black fungus was shown as a synergistic effect in Table 5, which was 98% by weight LV and 2% by weight CPC in the case of 0.13. To this mixture (hereinafter referred to as LCF mixture) and an appropriate amount of sodium citrate 60% by weight (see Table 6 below).

On the other hand, according to the results of Experimental Example 1 and Experimental Example 2, the most preferable weight ratio of levulinic acid, sodium citrate and cetylpyridinium mixture for removing Pseudomonas aeruginosa and Black fungus was 61-66: 2-13: 20-36 .

< Experimental Example  3: Wet tissue pH  Measurement>

The above Examples 1 to 2 and Comparative Examples 1 to 8 were subjected to various nonwoven fabrics used as raw materials for wet tissues, and then experiments were conducted to measure changes in pH. In this experiment, 100 nonwoven fabrics purchased separately by 100 sheets were laminated in 100 sheets, and then cut into a size of 14 x 20 cm. The pH of the extracted solution was measured first, and 15 g of water was used in comparison with 10 g of the nonwoven fabric. Thus, the pH of the extracted solution was measured using a 150% aqueous solution Were measured. The initial pH of the nonwoven fabric thus measured was 3.0 for nonwoven fabric 1, 6.2 for nonwoven fabric 2, 7.0 for nonwoven fabric 3, and 8.4 for nonwoven fabric 4. Thereafter, the compositions of Examples 1 to 2 and Comparative Examples 1 to 8 were treated with each of the nonwoven fabrics separately purchased by the same route, and the pH of the nonwoven fabric was measured in the same manner as the method of measuring the initial pH Respectively. In the above Examples 1 and 2, other components were also measured as in Example 3, and Comparative Examples 1 to 8 were also measured in the case of containing other components as in Comparative Example 9. [

The following Table 7 shows the measured pH values after treating the nonwoven fabrics of Examples 1 to 2 and Comparative Examples 1 to 8, respectively.

As can be seen from the results shown in Table 7, it was confirmed that the pH of Example 1 and Example 2 was fixed at 4.5-5.5 irrespective of the type of nonwoven fabric. In addition, in Examples 1 and 2, it was confirmed that even when other materials were added to the nonwoven fabric during the production of the wet tissue, the pH did not significantly change and stable and stable pH could be expressed.

However, in the case of Comparative Examples 1 to 8, it was confirmed that pH was expressed in various ranges depending on the kind of the nonwoven fabric and the type of the mixture. In particular, when either levulinic acid or sodium citrate was not added by Comparative Example 1 to Comparative Example 2, stable pH was not expressed as in Examples 1 and 2, and as a result of Comparative Examples 3 to 8 It was confirmed that stable pHs as in Examples 1 and 2 were not expressed when other organic acids were used in place of bourabulic acid or other basic substances used in wet tissues were used. It was also confirmed that the pH of Examples 1 and 2 was more stable than that of Comparative Examples 1 to 8 even when other additives used in other wet tissues were mixed as in Example 3 and Comparative Example 9.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. It is natural.

Claims (9)

Claims 1. A antimicrobial composition for a wet tissue comprising water, levulic acid, cetylpyridinium chloride, and sodium citrate,
Wherein the composition is characterized by comprising 0.15-0.30 parts by weight of levulinic acid, 0.02-0.04 parts by weight of cetylpyridinium chloride, and 0.05-0.20 parts by weight of sodium citrate, based on 100 parts by weight of water,
Wherein the pH of the solution extracted by pressing the nonwoven fabric of the wet tissue treated with the composition is 4-6 irrespective of the initial pH of the nonwoven fabric.
The method according to claim 1,
Wherein the composition further comprises lactic acid.
The method according to claim 1,
Wherein the composition has a ratio of levulinic acid: sodium citrate of 1: 1 to 1: 4.
delete 3. The method of claim 2,
Wherein the composition comprises 0.15-0.30 parts by weight of levulinic acid, 0.02-0.04 parts by weight of cetylpyridinium chloride, 0.05-0.20 parts by weight of sodium citrate, and 0.04-0.10 parts by weight of lactic acid per 100 parts by weight of water. .
1) mixing 0.15-0.30 parts by weight of levulinic acid, 0.02-0.04 parts by weight of cetylpyridinium chloride, and 0.05-0.20 parts by weight of sodium citrate with respect to 100 parts by weight of water to obtain a antimicrobial composition for a wet tissue; And
2) spraying the composition onto one or a plurality of nonwoven fabrics stacked thereon, followed by squeezing, or dipping one or more nonwoven fabrics stacked in the composition and pressing them;
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Wherein the pH of the solution extracted by pressing the nonwoven fabric of the wet tissue treated with the composition is 4-6 irrespective of the initial pH of the nonwoven fabric.
The method according to claim 6,
Wherein the composition of step 1) further comprises lactic acid.
The method according to claim 6,
Wherein the composition of step 1) comprises a ratio of levulinic acid: sodium citrate of 1: 1 to 1: 4.
The pH of the extracted solution was obtained by pressing a nonwoven fabric of a wet tissue treated with a composition containing 0.15-0.30 parts by weight of levulinic acid, 0.02-0.04 parts by weight of cetylpyridinium chloride and 0.05-0.20 parts by weight of sodium citrate with respect to 100 parts by weight of water Characterized in that it is 4-6 irrespective of the initial pH of the nonwoven fabric.