KR101582660B1 - Hydrogel Mask Pack Production Containing Collagen Using Radiation - Google Patents

Abstract

The present invention relates to a hydrogel mask pack containing ingredients such as collagen, biocompatible polymers, and natural polymers, which is manufactured by being exposed to radiation. The hydrogel mask pack containing collagen, according to the present invention, has physical properties of hydrogel by forming physical crosslinks using radiation, has an excellent antiseptic function without adding preservatives through simultaneous sterilization, and improves the ability to retain natural oils and moisture.

Description

(Hydrogel Mask Pack Production Containing Collagen Using Radiation)

The present invention relates to a collagen-containing hydrogel mask pack comprising collagen, a biocompatible polymer, a natural polymer, etc., and irradiated with radiation.

The greatest amount of skin can be said to be high temperature, high humidity, and ultraviolet rays. When our skin is exposed to hot and humid weather, the skin itself becomes less protective. This causes a lot of skin secretions such as sweat and sebum from the skin. Excessive perspiration and secretion of sebum widen the pores, causing the skin to sag. Skin irritation caused by ultraviolet rays causes stains, dullness, and freckles by skin pigmentation, and accelerates aging of skin. Therefore, appropriate skin care is needed.

First of all, if you have sweat and sebum secretions, you should wipe it immediately and wash it as often as possible to maintain a clean and refreshing sensation. However, frequent cleansing at this time causes skin to become dry and rough due to moisture loss, so water supply is necessary. Skin care that satisfies the absorption and hydration of skin secretion at the same time is required. Therefore, it is possible to absorb and remove the secretion of the skin because of its water absorbency, and it is effective to use a pack capable of supplying moisture to the skin because of its high moisture content.

Pack is a kind of functional cosmetics that protects the skin physiology function by keeping the blood circulation smooth by supplying the moisturizing and cosmetic ingredients to the skin effectively by wrapping the active ingredient on the face and wrapping the active ingredient in the face. Since the pack can provide various nutritional ingredients very effectively to the skin, various effects such as moisturizing, soothing, anti-aging, keratin and waste removal, tired skin recovery, elasticity enhancement and whitening can be expected by these cosmetic ingredients . In addition, when the pack is dried, the pack shrinks and the skin is pulled to form tension on the skin. In addition, the pack improves the elasticity of the skin due to the adhesive force and the massage effect when the film is peeled off, and can alleviate the fine lines. In addition to the skin cosmetic and health effects of the pack as described above, psychological stability that can relieve the stress accumulated by providing relaxation to the face and body tired by a comfortable mind during the time required for applying the pack to the skin and drying Effect can be expected.

Here, a hydrogel may be applied to the pack. Hydrogels have been mainly used for the purpose of biomedicine delivery system or skin wound healing. Recently, hydrogels applied to cosmetics have been developed. Hydrogels mainly use a hydrophilic polymer to facilitate skin penetration. The hydrophilic polymer can be suitably mixed with a branched type gelling polymer, an electrolytic gelated polymer, etc. to suit the shape of the product. In order to facilitate the delivery of the drug to the skin, to be.

Examples of the branched gelatinous polymers may include galactomannan, glucomannan, guar gum, rocastin bean gum, pluronic and the like, and the electrolytic gel polymer may be agar, algin, carrageenan, xanthan and gellan. They can further improve the intimacy, adhesion and cosmetic effects of the skin by further blending ingredients such as formulation and moisturizing agent at an appropriate temperature.

Such polymers used in hydrogels of cosmetics, such as gums, are rubbery substances present in plants and microorganisms, rubbery substances and mucous substances secreted from plants and microorganisms, and are highly viscous and have polysaccharides with distinctive properties It says.

Are black structurally hydrophilic (polar) or hydrophobic (nonpolar) molecules with a polymeric weight and usually exhibit colloidal properties and can form gels or produce high viscosity dispersions or solutions at low concentrations under the use of suitable solvents.

However, in general, a sword refers to a plant or microbial polysaccharide or a derivative thereof which forms a viscous solution by dispersing in cold water or hot water. These include plant exudate gums, seaweed gums exuded from seaweed, seed gums exuded from plant seeds, starch and cellulose dervaties, microorganisms And microbial gums.

Agar, carrageenan, xanthan gum and the like are mainly used as swords. Agar does not dissolve in cold water but dissolves at 90 ℃ or higher to form a colloidal solution. The characteristics of the agar are that it has a strong gelation force, forms firm gels of brittle tissues and has a high melting point which is not well dissolved.

Carrageenan is a carrageen or carrageenan in the patent literature on the separation and purification of irish moss from 1837 to 1871, beginning with the extraction of Irish moss by residents of IRELAND beach, County Carragheen, 600 years ago It started to be used for the first time. Today, carrageenan is used in hundreds of products, from food to toothpaste, air fresheners, and industrial suspensions. Carrageenan, which is produced from red seaweed, is composed of β-D-galactose linked by β-1,3 bond and α-D-galactose linked by α-1,4 bond as repeating units. Structure. Unlike natural conditions, alkali treatment is required for commercial purposes and the difference in structural changes due to Kappa-type, lota-type, and Lambda-type depending on the alkali treatment appear.

The hydrogel composition used in cosmetics is prepared by mixing agar, carrageenan, xanthan gum, etc. with a moisturizing agent, a pH adjuster, a metal ion sequestering agent and the like, and gelling the mixture using appropriate 1 to 2 cations.

Such a hydrogel composition exhibits various characteristics, that is, water release, in which a large amount of solvent (a solvent of a water-soluble substance including water) is adsorbed and discharged, The cosmetic ingredients contained in the solvent give the skin a cosmetic effect.

Patent Document 1 discloses a skin cosmetic packaged product of a hydrogel and a hydrogel including a composition comprising polyvinyl pyrrolidone (PVP), polyhydric alcohol and carrageenan having excellent biocompatibility.

At this time, collagen may be applied to the hydrogel pack. Collagen is the main protein of animal connective tissues. It occupies more than 30% of pre-vital proteins. It mainly maintains the tissues and organs while keeping the body shape. It keeps more than 90% [AK Piez, Encyclopedia of Polymer Science and Engineering Collagen, in: JI Koroschwitz (ed.), New York, 699 (1985)]. Collagen has been active since 1950, with its physicochemical, biochemical and biological studies, like other industrially used proteins. As a result, the discovery of soluble collagen peptides has led to rapid progress in collagen research, and collagen is an important material in the development of medicines, health functional foods and cosmetics [T. Miyata, Fragrance Journal, 17, 90 (1989)]. Collagen is a fibrous protein that accounts for about 70% of the dermal layer of the skin. It is synthesized naturally in the human body, but as the age increases, the amount produced is reduced, which causes wrinkles. As a result of numerous studies on the effect of wrinkles on collagen, collagen has been proved to have a great effect on improving wrinkles of collagen, and collagen has started to be used as a raw material for cosmetics.

Patent Document 2 discloses the content of opaque hydrogel cosmetic pack composition using a marine collagen material.

Currently, mask packs using collagen are marketed and distributed, but they are oxidized when stored for a long period of time, which makes it difficult to distribute them for a long time due to deterioration of color and deterioration of physical properties. In addition, preservatives such as methylparaben, propylene paraben, phenoxyethanol, and hexanediol, and metal salts such as calcium salts, magnesium salts, barium salts, and aluminum salts may cause skin irritation.

Accordingly, the inventors of the present invention have attempted to develop a hydrogel mask pack containing collagen improved in the above problems, irradiate the collagen-containing hydrogel mask pack with radiation to impart physical properties of the hydrogel through physical crosslinking, The present inventors not only have excellent buoyant power but also improved skin moisture and oil retaining ability without adding preservatives, thus completing the present invention.

Korean Patent No. 10-2003-0020422 Korean Patent 10-2012-0057914

An object of the present invention is to provide a collagen-containing hydrogel prepared by irradiating radiation.

Another object of the present invention is to provide a method for producing a mask pack comprising the collagen-containing hydrogel.

It is still another object of the present invention to provide a mask pack comprising the collagen-containing hydrogel.

Another object of the present invention is to provide a dressing for wound healing comprising the collagen-containing hydrogel.

It is still another object of the present invention to provide a dressing for burn treatment comprising the collagen-containing hydrogel.

In order to achieve the above object,

The present invention provides a collagen-containing hydrogel comprising collagen, a biocompatible polymer and a natural polymer, which is produced by irradiating the collagen.

In addition, the present invention relates to a cosmetic composition comprising 100 parts by weight of collagen,

20 to 100 parts by weight of a biocompatible polymer;

1 to 30 parts by weight of a natural polymer; And

And 700 to 900 parts by weight of purified water, wherein the collagen-containing hydrogel is prepared by irradiation with radiation.

Furthermore, the present invention relates to a method for manufacturing a biocompatible polymer and a natural polymer, which comprises quantitatively weighing a biocompatible polymer and a natural polymer and mixing them in a powder state to obtain a raw material mixture (step 1);

Adding the raw material mixture obtained in step 1 to purified water, dispersing the mixture using a homomixer and warming to obtain a mixture (step 2);

Adding collagen to the mixture obtained in step 2, and dispersing it with a homomixer to obtain a crude liquid (step 3);

Coating the release solution obtained in step 3 in a pregelled state on a release paper (step 4); And

(5) after completion of the molding of the pregel obtained in the step (4), sealing it in a pouch without air circulation and irradiating it with radiation (step 5).

In addition, the present invention provides a skin-beauty mask pack comprising the collagen-containing hydrogel.

Furthermore, the present invention provides a wound dressing comprising the collagen-containing hydrogel.

The present invention also provides a dressing for burn treatment comprising the collagen-containing hydrogel.

The collagen-containing hydrogel mask pack according to the present invention imparts the physical properties of the hydrogel by physical crosslinking through irradiation, and has sterilizing effect simultaneously, so that it has excellent repellency without addition of a preservative, There is an effect to be improved.

FIG. 1 is a photograph of the mask pack of Example 4 prepared through irradiation with radiation and the repelling force of Comparative Example 1 having the same composition but not irradiated with radiation.

Hereinafter, the present invention will be described in detail.

The present invention provides a collagen-containing hydrogel comprising collagen, a biocompatible polymer and a natural polymer, which is produced by irradiating the collagen.

In addition, the present invention relates to a cosmetic composition comprising 100 parts by weight of collagen,

20 to 100 parts by weight of a biocompatible polymer;

1 to 30 parts by weight of a natural polymer; And

And 700 to 900 parts by weight of purified water, wherein the collagen-containing hydrogel is prepared by irradiation with radiation.

In the composition of the collagen-containing hydrogel according to the present invention, the collagen is at least one selected from the group consisting of donkey collagen, hair collagen, green tea collagen, fish scale collagen and fish skin collagen .

In addition, in the composition of the collagen-containing hydrogel according to the present invention, the biocompatible polymer may be at least one selected from the group consisting of polyvinylpyrrolidone, polyvinyl alcohol, polyacrylic acid, polyethylene oxide, 1-vinylimidazole, , And polyvinyl pyrrolidone can be preferably used. More specifically, polyvinylpyrrolidone is a linear hydrophilic polymer composed of a 1-vinyl-2-pyrrolidone monomer having a structure represented by the following general formula (1), and is a biocompatibility- It is used as a biomaterial and has recently been actively studied as a substitute for ocular vitreous. In the present invention, the polyvinylpyrrolidone has oxygen and nitrogen in its unit structure and forms a hydrogen bond with water molecules, so that it can contain a large amount of water in the network structure and is suitable for producing a hydrogel.

[Chemical Formula 1]

In Formula 1,

x is an integer from 100 to 1,000,000.

The polyvinylpyrrolidone may be used in an amount of 20 to 100 parts by weight, preferably 30 to 50 parts by weight, and more preferably 40 parts by weight, based on 100 parts by weight of the collagen. When the amount of the polyvinylpyrrolidone is less than 20 parts by weight based on 100 parts by weight of the collagen, the biocompatibility is poor, the cross-linking reaction by radiation does not occur, and the viscosity of the polymer solution is very low. The polyvinylpyrrolidone may have a mechanical property that is inappropriate for use as a hydrogel pack, and when the polyvinylpyrrolidone is used in an amount of more than 100 parts by weight, it may be difficult to produce a high concentration aqueous solution and excess polyvinylpyrrolidone The strength of the hydrogel is increased due to the high crosslinking ratio due to the radiation contained in the hydrogel, and the physical properties of the hydrogel to be applied to the mask pack may be deteriorated.

Furthermore, in the composition of the collagen-containing hydrogel according to the present invention, the natural polymer may be a mixture of at least one selected from the group consisting of carrageenan, xanthan gum, sodium carboxymethyl cellulose, gelatin, agar, alginate and chitosan And preferably carrageenan and xanthan gum may be mixed and used.

Carrageenan is a plant material that can be obtained from a variety of red algae belonging to the family Gigar-thinaceae or Solieriaceae, and is mainly used as a gelling agent. More specifically, the carrageenan may be used in an amount of 1 to 20 parts by weight, preferably 5 to 15 parts by weight, and more preferably 10 parts by weight, based on 100 parts by weight of the collagen. If the content of carrageenan is less than 1 part by weight based on 100 parts by weight of the collagen, the gelation ability of the hydrogel may deteriorate and the strength to maintain the shape of the hydrogel before irradiation may be lowered. If the content of carrageenan is 20 parts by weight , There is a problem that excessive hydrogels are contained in excess of the required amount of the carrageenan to lower the physical properties of the hydrogel to be applied to the mask pack and may interfere with crosslinking of polypyrrolidone upon irradiation with radiation.

Xanthan Gum is a high molecular weight heteropolysaccharide gum obtained by pure fermentation of carbohydrates with Xanthomonas campestris. It is mainly composed of binders, emulsifying stabilizers, skin conditioners, surfactants-emulsifiers, - Water soluble. More specifically, 1 to 10 parts by weight, preferably 3 to 7 parts by weight, and more preferably 5 parts by weight can be used for 100 parts by weight of the black xanthan gum black collagen. When the amount of the xanthan gum is less than 1 part by weight based on 100 parts by weight of the collagen, the viscosity of the raw material mixture (polyvinylpyrrolidone, carageenan, etc.) of the present invention is low and it is difficult to handle, If the amount of the xanthan gum is more than 10 parts by weight, the coating liquid may have a viscosity higher than necessary, so that the physical properties of the hydrogel to be applied to the mask pack may deteriorate. When the amount of polypyrrolidone There is a problem that it may be a factor that interferes with the crosslinking.

Also, in the composition of the collagen-containing hydrogel according to the present invention, the purified water serves to dissolve the raw materials such as polyvinylpyrrolidone, carrageenan, xanthan gum, collagen and the like to facilitate inclusion in the hydrogel. The purified water may be used in an amount of 700 to 900 parts by weight, preferably 820 to 870 parts by weight, more preferably 840 to 850 parts by weight, based on 100 parts by weight of the collagen. If the purified water is less than 700 parts by weight based on 100 parts by weight of the collagen, there may arise a problem that the crude solution concentration of the raw material mixture of the present invention (polyvinylpyrrolidone, carrageenan, xanthan gum, etc.) When the purified water is used in an amount of more than 900 parts by weight, the concentration of the crude solution is greatly diluted and the physical properties of the hydrogel to be applied to the mask pack are lowered , Which may interfere with crosslinking of polypyrrolidone upon irradiation with radiation.

The collagen-containing hydrogel may further contain additives. The additives may be at least one selected from the group consisting of a preservative, a bactericide, a fluorescent whitening agent, a pigment, a stabilizer, a pH adjusting agent and an antioxidant .

Further, as the radiation used for the radiation, usual gamma rays or electron-beams may be used. In this case, the dose of radiation may be 10-100 kGy, preferably 15 -50 kGy, more preferably 20-30 kGy, even more preferably 25 kGy. When the irradiation dose is less than 10 kGy, sterilization may not be performed well, and crosslinking of the polymer may not be performed, so that it is difficult to prepare the hydrogel. When the irradiation dose is more than 100 kGy, But it is easily broken due to the lack of flexibility and the cross-linking ratio of the polymer is increased, so that the hydrogel can not be easily obtained. Here, the dose rate can be 1-50 kGy / hr, preferably 5-20 kGy / hr, more preferably 8-12 kGy / hr.

In addition, the present invention relates to a method for producing a biocompatible polymer and a natural polymer, which comprises quantitatively weighing a biocompatible polymer and a natural polymer and mixing them in powder form to obtain a raw material mixture (step 1);

Adding the raw material mixture obtained in step 1 to purified water, dispersing the mixture using a homomixer and warming to obtain a mixture (step 2);

Adding collagen to the mixture obtained in step 2, and dispersing it with a homomixer to obtain a crude liquid (step 3);

Coating the release solution obtained in step 3 in a pregelled state on a release paper (step 4); And

(5) after completion of the molding of the pregel obtained in the step (4), sealing it in a pouch without air circulation and irradiating it with radiation (step 5).

Hereinafter, the method for producing the collagen-containing hydrogel mask pack according to the present invention will be described in detail.

In the method for producing a collagen-containing hydrogel mask pack according to the present invention, the step 1 is a step of quantitatively weighing a biocompatible polymer and a natural polymer and mixing them in powder form to obtain a raw material mixture. More specifically, 100 parts by weight 20 to 100 parts by weight of a biocompatible polymer and 1 to 30 parts by weight of a natural polymer are quantitatively weighed and mixed in a powder state to obtain a raw material mixture.

At this time, it is preferable to mix all the raw materials so as to be uniformly mixed.

In the method for producing a collagen-containing hydrogel mask pack according to the present invention, the step 2 is a step of adding the raw material mixture obtained in the above step 1 to purified water, dispersing it using a homomixer, and warming to obtain a mixture, The raw material mixture obtained in the step 1 is added to purified water at room temperature and heated at 50 to 100 DEG C while dispersing it at 1000 to 2000 rpm in a homomixer, and mixing is preferably performed for 10 to 20 minutes.

In the method for producing a collagen-containing hydrogel mask pack according to the present invention, the step 3 is a step of adding collagen to the mixture obtained in the step 2 and dispersing the mixture with a homomixer to obtain a crude liquid. More specifically, , 100 parts by weight of collagen is added thereto in a quantitative manner, and the resulting mixture is heated to 50 to 100 DEG C while being dispersed in a homomixer at 1000 to 2000 rpm and mixed for 10 to 20 minutes.

In the method for producing a collagen-containing hydrogel mask pack according to the present invention, the step 4 is a step of coating the release liquid obtained in the above step 3 onto a release paper in a pregel state. More specifically, The coating solution is coated at a thickness of about 1 to 2 mm on a peeling paper which is easy to peel off and is cooled at a low temperature (5-10 ° C) to obtain a pregel (a hydrogel in a state of having a physical property, Gel) to complete the coating.

In the method of manufacturing a collagen-containing hydrogel mask pack according to the present invention, the step 5 is a step of sealing the pregel after the molding of the pregel obtained in the step 4 is completed, sealing it in a pouch without air circulation, More specifically, the pregel obtained in the step 4 is cut into a wooden form (face, neck, eyes, etc.) prepared according to the purpose of use to complete the molding, and then put in a pouch without air circulation to completely seal the pregel, And the step of irradiating the radiation.

As the radiation used for the radiation, usual gamma-rays or electron-beam may be used. In this case, the dose of radiation may be 10-100 kGy, preferably 15-50 kGy, more preferably 20-30 kGy, even more preferably 25 kGy. When the irradiation dose is less than 10 kGy, the sterilization may not be performed well, and crosslinking of the polymer may not be performed, so that hydrogel production is difficult. When the irradiation dose is more than 100 kGy, the gel strength is increased However, it is easily broken due to the lack of flexibility, and the cross-linking ratio of the polymer is increased, so that hydrogel can not be easily obtained. Here, the dose rate can be 1-50 kGy / hr, preferably 5-20 kGy / hr, more preferably 8-12 kGy / hr.

Furthermore, the present invention provides a mask pack for use in skin care comprising the collagen-containing hydrogel.

In addition, the present invention provides dressing for wound healing comprising the collagen-containing hydrogel.

Further, the present invention provides a dressing for burn treatment comprising the collagen-containing hydrogel.

At present, collagen-containing hydrogel mask packs are commercially available and circulated, but they are oxidized when stored for a long period of time, which makes it difficult to circulate them for a long time due to deterioration of color and physical properties. Preservatives such as methylparaben, propylene paraben, phenoxyethanol, hexanediol, and metal salts such as calcium salts, magnesium salts, barium salts and aluminum salts may also cause skin irritation. Thus, in the present invention, it is expected that the hydrogel mask pack containing collagen not only imparts the physical properties of the hydrogel through physical crosslinking by irradiation with radiation, but also has an excellent repelling force without sterilization and without adding preservatives. In addition, since the collagen particle size is reduced due to radiation, the collagen is more uniformly dispersed than a general collagen-containing mask pack, and the collagen absorption rate is expected to be better when applied to the skin.

First, as a result of conducting an experiment to evaluate the gelation rate of the collagen-containing hydrogel according to the present invention, it was confirmed that the collagen-containing hydrogel of Example 1-4 according to the present invention had an excellent gelation rate (%) See Table 2 in Experimental Example 1).

The collagen-containing hydrogel of Example 1-4 according to the present invention was found to exhibit excellent tensile strength as a result of an experiment to evaluate the tensile strength of the collagen-containing hydrogel mask pack according to the present invention. More specifically, when comparing the tensile strengths of the mask packs of Example 4 produced through irradiation with radiation and Comparative Example 1 having the same composition but not irradiated with radiation, the mask packs of Example 4 (0.26 kgf / cm ² ) Had a higher tensile strength than the mask pack (0.22 kgf / cm ² ) of Comparative Example 1 (see Table 3 in Experimental Example 2).

Further, as a result of conducting an experiment to evaluate the moisture content of the collagen-containing hydrogel mask pack according to the present invention, it was confirmed that the collagen-containing hydrogel of Example 1-4 according to the present invention exhibited an excellent moisture content. More specifically, when the moisture content (%) of Comparative Example 1 having the same composition but not irradiated with radiation was compared with the mask pack of Example 4 prepared through irradiation with radiation, the mask pack of Example 4 (94.81% ) Had a water content (%) superior to that of the mask pack (90.27%) of Comparative Example 1 (see Table 4 in Experimental Example 3).

In addition, when the collagen-containing hydrogel mask pack according to the present invention was used, an experiment was conducted to evaluate the water loss rate (%) of adhered skin. As a result, the collagen- When the hydrogel mask pack was used, the water loss rate (%) with time was lower than that of Comparative Example 1 and Comparative Example 2. More specifically, when comparing the moisture loss rate (%) of the mask pack of Example 4 prepared through irradiation with radiation and Comparative Example 1 having the same composition but not irradiated with radiation, the mask pack of Example 4 was compared with the mask pack of Comparative Example 1 (see Table 5 in Experimental Example 4).

This shows that the collagen particle size is reduced due to radiation, and collagen is dispersed more uniformly than a general collagen-containing mask pack, resulting in an increase in collagen absorption rate when applied to skin.

Furthermore, when the collagen-containing hydrogel mask pack according to the present invention was used, experiments were conducted to evaluate the oil content (%) of adhered skin. As a result, the collagen-containing hydrogel mask pack prepared in Example 1-4 according to the present invention When the gel mask pack was used, the oil content (%) over time was superior to that of Comparative Example 1 and Comparative Example 2. More specifically, when the mask pack of Example 4 manufactured by irradiation with radiation and the oil content (%) of Comparative Example 1 having the same composition but not irradiated with radiation were compared, the mask pack of Example 4 was compared with the mask pack of Comparative Example 1 (%) Of the skin was significantly superior to that of the mask pack (see Table 6 of Experimental Example 5).

This shows that the collagen particle size is reduced due to radiation, and collagen is dispersed more uniformly than a general collagen-containing mask pack, resulting in an increase in collagen absorption rate when applied to skin.

In addition, as a result of conducting an experiment to evaluate the flushing force of the collagen-containing hydrogel mask pack according to the present invention, it was found that the mask pack of Comparative Example 1 in which no radiation was applied produced spoilage due to mold, In the mask pack of Example 4, it was found that corruption due to mold was not caused by the effect of sterilization by radiation (see FIG. 1 of Experimental Example 6).

It can be seen that the sterilization is performed simultaneously due to the radiation, which is a result of having excellent repelling force without addition of preservative.

Therefore, the collagen-containing hydrogel mask pack prepared through irradiation with radiation according to the present invention has improved collagen absorption rate on the skin as compared with a collagen-containing mask pack not irradiated with radiation, so that not only the water retention (moisturizing) And it is possible to prevent decay of the product owing to the effect of sterilization by radiation without addition of an antiseptic, so that it can be effectively used for the purpose of a dressing or a mask pack having excellent performance.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the present invention is not limited to the following Examples and Experimental Examples.

< Example  1-5> Collagen content Hydrogel  Manufacture of mask pack

The ingredients of the composition shown in Table 1 below were mixed to prepare a collagen-containing hydrogel mask pack. More specifically, polyvinylpyrrolidone (PVP), carrageenan and xanthan gum were added to purified water at room temperature, and the mixture was heated to 65 DEG C while being dispersed at 2000 rpm in a homomixer. The mixture was further mixed for 10 minutes from the time when the temperature of the solution reached 65 占 폚. Thereafter, the crushed pork (collagen) was quantitatively added to the composition shown in Table 1, and the mixture was dispersed at 2000 rpm while maintaining the temperature at 65 캜 for 10 minutes to obtain a crude liquid.

The crude liquid obtained through the above process was coated on the release paper, which is easy to peel, to a thickness of about 1 to 2 mm. Coating was carried out at low temperature (5 ~ 10 ℃) and the coating was completed with the pregel (hydrogel in the state that the polymer had physical property that is easy to form before radiation crosslinking).

The pregel obtained through the above process was cut into a wooden form (face, neck, eyes, etc.) manufactured according to the purpose of use, and after completion of molding, it was put in a pouch without air circulation and completely sealed.

Finally, for the cross-linking and sterilization of polymers, irradiation with radiation was completed to prepare the hydrogel. At this time, the radiation source used for irradiation is cobalt-60 with a dose of 25 kGy and a dose rate of 10 kGy / hr.

Weight portion Collagen PVP Carrageenan Xanthan gum Purified water Cross-linking agent Irradiation Example 1 100 100 20 10 770 - O Example 2 100 80 20 10 790 - O Example 3 100 60 10 5 825 - O Example 4 100 40 10 5 845 - O Example 5 100 20 10 5 865 - O Comparative Example 1 100 40 10 5 845 5 X Comparative Example 2 - 40 10 5 845 - O

(In Table 1,

The crosslinking agent may be any chemical crosslinking agent selected from the group consisting of boric acid, dialdehyde, dicarboxylic acid, dianhydride, hydrochloric acid and epichlorohydrin.

< Experimental Example  1> Gelation rate  evaluation

In order to evaluate the gelation rate of the collagen-containing hydrogel prepared in Example 1-5 according to the present invention, the following experiment was conducted.

The collagen-containing hydrogel mask pack prepared in Example 1-5 and Comparative Example 1-2 was sectioned to a size of 1 cm x 5 cm x 0.01 m, and the gelation rate of the section was calculated by the following equation 1 to measure the gelation rate Respectively. The gelation rate is based on important physical properties that determine the physical properties of the hydrogel. The higher the gelation rate, the lower the moisture content and the gel strength becomes stronger. The results are shown in Table 2 below.

Gelation rate (%) Example 1 52% Example 2 48% Example 3 43% Example 4 41% Example 5 0 % Comparative Example 1 40% Comparative Example 2 41%

As shown in Table 2, it was confirmed that the collagen-containing hydrogel of Example 1-4 according to the present invention exhibited excellent gelation rate (%).

Thus, the collagen-containing hydrogel prepared through irradiation with radiation according to the present invention has a good gelation rate (%), and thus can be effectively used as a dressing for treatment or a mask pack having excellent performance.

< Experimental Example  2> The tensile strength  evaluation

In order to evaluate the tensile strength of the collagen-containing hydrogel mask pack prepared in Example 1-4 according to the present invention, the following experiment was conducted.

The collagen-containing hydrogel mask pack prepared in Example 1-4 and Comparative Example 1-2 was cut into pieces of 1 cm × 5 cm × 0.01 m and mounted on a Universal Tester Machine (Vitridyne V-1000, LIVECO Inc., USA) Respectively. The initial sample measurement distance was set at 10 mm and the pulling speed in both directions was measured at 100 μm / s. At this time, the point at which the section was broken was defined as the maximum elongation length. Each experiment was repeated an average of 3 times to calculate a mean value, and the results are shown in Table 3 below.

Tensile strength (kgf / cm ² ) Maximum height (cm) Example 1 0.57 3.6 Example 2 0.46 3.5 Example 3 0.32 3.7 Example 4 0.26 4.1 Comparative Example 1 0.22 4.9 Comparative Example 2 0.26 4.1

As shown in Table 3, the collagen-containing hydrogel of Example 1-4 according to the present invention was found to exhibit excellent tensile strength. More specifically, when comparing the tensile strengths of the mask packs of Example 4 produced through irradiation with radiation and Comparative Example 1 having the same composition but not irradiated with radiation, the mask packs of Example 4 (0.26 kgf / cm ² ) Had a tensile strength higher than that of the mask pack (0.22 kgf / cm ² ) of Comparative Example 1.

Therefore, the collagen-containing hydrogel prepared through irradiation with radiation according to the present invention has a superior tensile strength as compared with the collagen-containing hydrogel not irradiated with radiation, so that it can be effectively used as a treatment dressing or a mask pack having improved durability have.

< Experimental Example  3> Water content evaluation

In order to evaluate the water content of the collagen-containing hydrogel mask pack prepared in Example 1-4 according to the present invention, the following experiment was conducted.

The collagen-containing hydrogel mask pack prepared in Example 1-4 and Comparative Example 1-2 was cut into pieces of size 1 cm × 5 cm × 0.01 m, and a moisture analyzer (MX-50, AND, Japan) was used to measure the moisture content. The results are shown in Table 4 below.

Moisture content (%) Example 1 90.01 Example 2 91.72 Example 3 92.43 Example 4 94.81 Comparative Example 1 90.27 Comparative Example 2 91.32

As shown in Table 4, it was confirmed that the collagen-containing hydrogel of Example 1-4 according to the present invention exhibited excellent moisture content. More specifically, when the moisture content (%) of Comparative Example 1 having the same composition but not irradiated with radiation was compared with the mask pack of Example 4 prepared through irradiation with radiation, the mask pack of Example 4 (94.81% ) Had a water content (%) superior to that of the mask pack (90.27%) of Comparative Example 1.

Therefore, the collagen-containing hydrogel produced through irradiation with radiation according to the present invention is superior in moisture content (%) as compared with the collagen-containing hydrogel not irradiated with radiation, so that the therapeutic dressing or mask Packs.

< Experimental Example  4> Evaluation of skin moisture loss rate (%)

(Skin-o-Mat (cosmomed, Germany)) was used to evaluate the water loss rate (%) of the adhered area over time after using the collagen-containing hydrogel mask pack prepared in Example 1-4 according to the present invention. (TEWL, Trans Epidermal Water loss) was measured by using the equipment. The results are shown in Table 5 below.

20 minutes 40 minutes 60 minutes 80 minutes 100 minutes Example 1 20% 23% 24% 38% 49% Example 2 19% 22% 23% 38% 49% Example 3 19% 20% 22% 37% 48% Example 4 18% 20% 21% 35% 48% Comparative Example 1 25% 28% 32% 43% 56% Comparative Example 2 30% 35% 42% 50% 61%

As shown in Table 5, when the collagen-containing hydrogel mask pack prepared in Example 1-4 according to the present invention was used, the water loss rate (%) over time was lower than that of Comparative Example 1 and Comparative Example 2 Respectively. More specifically, when comparing the moisture loss rate (%) of the mask pack of Example 4 prepared through irradiation with radiation and Comparative Example 1 having the same composition but not irradiated with radiation, the mask pack of Example 4 was compared with the mask pack of Comparative Example (%) Was significantly lower than that of the mask pack of No. 1.

Therefore, the collagen-containing hydrogel produced through irradiation with radiation according to the present invention is excellent in the ability to suppress skin moisture loss as compared with the collagen-containing hydrogel not irradiated with radiation, For example, a mask dressing, or a mask pack.

< Experimental Example  5> Skin Oil content (%) evaluation

After using the collagen-containing hydrogel mask pack prepared in Example 1-4 according to the present invention, skin-o-mat (cosmomed, Germany) equipment was used to evaluate the amount of oil The results are shown in Table 6 below.

20 minutes 40 minutes 60 minutes 80 minutes 100 minutes Example 1 84% 79% 66% 59% 57% Example 2 84% 79% 68% 59% 58% Example 3 85% 80% 68% 61% 60% Example 4 86% 80% 70% 62% 60% Comparative Example 1 78% 70% 62% 55% 49% Comparative Example 2 65% 58% 51% 46% 40%

As shown in Table 6 above, when the collagen-containing hydrogel mask pack prepared in Example 1-4 according to the present invention was used, the oil content (%) over time was superior to that of Comparative Example 1 and Comparative Example 2 appear. More specifically, when the mask pack of Example 4 manufactured by irradiation with radiation and the oil content (%) of Comparative Example 1 having the same composition but not irradiated with radiation were compared, the mask pack of Example 4 was compared with the mask pack of Comparative Example 1 (%) Than the mask pack of the present invention.

Therefore, the collagen-containing hydrogel prepared through irradiation with radiation according to the present invention is excellent in the ability to preserve the skin oil content (%) as compared with the collagen-containing hydrogel not irradiated with radiation, It can be usefully used as a therapeutic dressing or a mask pack having excellent holding power.

< Experimental Example  6> Buoyancy  evaluation

In order to comparatively evaluate the repellency of the mask pack of Example 4 prepared by irradiation with radiation and the repellency of Comparative Example 1 which had the same composition but was not irradiated with radiation, a mask pack was prepared by the same method as described above and stored at room temperature for 7 days After that, the pouch was opened to confirm the preservation state. The results are shown in Fig.

As shown in FIG. 1, in the mask pack of Comparative Example 1 in which radiation was not irradiated, mold spoilage occurred. On the other hand, in the mask pack of Example 4 prepared through irradiation with radiation, due to the effect of sterilization by radiation, Corruption did not occur.

Therefore, the collagen-containing hydrogel produced through irradiation with radiation according to the present invention has an effect of sterilizing by irradiation, and it is possible to prevent corruption during storage and distribution of the product without addition of preservative, It can be advantageously used as a mask dressing or dressing with excellent performance.

Claims (17)

delete With respect to 100 parts by weight of collagen,
40 to 100 parts by weight of a biocompatible polymer;
1 to 30 parts by weight of a natural polymer; And
700 to 900 parts by weight of purified water,
Wherein the biocompatible polymer is at least one member selected from the group consisting of polyvinyl pyrrolidone, polyvinyl alcohol, polyacrylic acid, polyethylene oxide, 1-vinylimidazole, and poly N-vinylimidazole,
The natural polymer may be at least one selected from the group consisting of carrageenan, xanthan gum, sodium carboxymethyl cellulose, gelatin, agar, alginate and chitosan. Since the natural polymer is prepared by irradiating with radiation, Wherein the collagen-absorbing amount of the collagen-containing hydrogel is improved.
3. The method of claim 2,
Wherein the collagen is at least one selected from the group consisting of pork collagen, whey collagen, green tea collagen, fish scale collagen, and fish skin collagen.
delete delete 3. The method of claim 2,
Wherein the radiation is one selected from the group consisting of gamma rays, ultraviolet rays, and electron beams.
3. The method of claim 2,
Wherein the irradiation dose of the radiation is 10-100 kGy and the dose rate is 1-50 kGy / hr.
A biocompatible polymer and a natural polymer are quantitatively weighed and mixed in a powder state to obtain a raw material mixture (step 1);
Adding the raw material mixture obtained in step 1 to purified water, dispersing the mixture using a homomixer and warming to obtain a mixture (step 2);
Adding collagen to the mixture obtained in step 2, and dispersing it with a homomixer to obtain a crude liquid (step 3);
Coating the release solution obtained in step 3 in a pregelled state on a release paper (step 4); And
(Step 5) after completion of the molding of the pregel obtained in step 4, sealing it in a pouch without air circulation and irradiating it with radiation,

The crude solution of step 4 is added to 100 parts by weight of collagen,
40 to 100 parts by weight of a biocompatible polymer;
1 to 30 parts by weight of a natural polymer; And
700 to 900 parts by weight of purified water,

Wherein the biocompatible polymer is at least one member selected from the group consisting of polyvinyl pyrrolidone, polyvinyl alcohol, polyacrylic acid, polyethylene oxide, 1-vinylimidazole, and poly N-vinylimidazole,
Wherein the natural polymer is at least one kind selected from the group consisting of carrageenan, xanthan gum, sodium carboxymethyl cellulose, gelatin, agar, alginate and chitosan.
delete 9. The method of claim 8,
Wherein the collagen is at least one selected from the group consisting of donkey collagen, whey collagen, green tea collagen, fish scale collagen, and fish skin collagen.
delete delete 9. The method of claim 8,
Wherein the radiation is one selected from the group consisting of gamma rays, ultraviolet rays, and electron beams.
9. The method of claim 8,
Wherein the irradiation dose of the radiation is 10-100 kGy and the dose rate is 1-50 kGy / hr.
A skin-beauty mask pack comprising the collagen-containing hydrogel of claim 2.
A wound dressing comprising the collagen-containing hydrogel of claim 2.
A dressing for burn treatment comprising the collagen-containing hydrogel according to claim 2.

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