KR101548483B1 - Bacillus subtilis sw002 - Google Patents

Abstract

The present invention relates to a novel strain of Bacillus subtilis, specifically, to a novel strain of Bacillus subtilis having an enzyme activity, acid resistance, bile acid tolerance and an antibacterial activity, and to a microbial agent thereof. The present invention can be used as a fermented agent for an enzyme, a livestock feed additive, production of a fermentation feed, fermentation of excrement, a soil conditioner, a fermented agent for herb medicines, and a water quality improvement agent, etc.

Description

Bacillus subtilis sw002 (accession number: KACC92034P) {Bacillus subtilis sw002}, which has excellent insecticidal activity against insoluble phosphatase and antibiotic resistance against staphylococci,

The present invention relates to a novel strain of Bacillus subtilis, and more particularly to a new strain Bacillus subtilis having enzyme activity, acid resistance, bile acid resistance and antimicrobial activity and a microorganism preparation thereof.

Today, agriculture causes excessive use of chemical fertilizers and pesticides, accumulation of salts, and so on. Biological treatments that can reduce or replace the use of chemical fertilizers or pesticides are emerging. In particular, it has been proven by domestic and foreign researchers that changes in soil ecosystems and techniques for biological control are possible by microorganisms.

In other words, in order to sustain agricultural productivity, it is necessary to secure the soundness of soil. Above all, it is the overuse of chemical fertilizer and pesticide, which is a major factor that threatens the sustainability of agriculture.

In particular, the use of chemical fertilizers not only acidifies the soil but also reduces the potential agricultural productivity by aggravating the physical properties of the soil by the accumulation of salts in the soil.

The Bacillus sp. Strain is a gram-positive bacterium and can be easily isolated in soil. In addition, the use of various degrading enzymes not only solves the accumulation of salts in the soil but also has the advantage of having antifungal activity by using various antimicrobial substances secreted from the cell, and thus it has become a subject of interest in the development of soil microbial preparations .

However, only a Bacillus subsp. Strain having only the above-mentioned merits mentioned above is known, and a strain showing the effect of solubilizing phosphorus, which is an essential nutrient element that is very important for the growth and development of plants, Is not known.

In other words, most of the phosphorus present in the soil exists as a chemically solidly insoluble complex or an organically bound phytate, and is present as an insoluble phosphate which the plant can not use at all.

Therefore, in order to improve the productivity of crops, the application of excessive inorganic phosphoric acid fertilizer to agricultural land is repeated.

The excess supply of phosphoric acid is absorbed only by the crops and the rest is moved to the surface layer of the soil together with the water, so that the water evaporates and accumulates only the salts, which makes the soil as a soil which does not grow properly. Soils with excessive concentrations of salts increase the risk of infectious diseases by interfering with the nutrient uptake and growth of the plants, causing eutrophication of surface waters and groundwater contamination.

On the other hand, probiotics, feed additives, environmental remedies, and soil microbial fertilizers using microorganisms such as those known in Patent Documents 0001 to 0006 and the like utilize beneficial bacteria such as Bacillus, Yeast, Fungus, Lactobacillus, To improve the efficiency of livestock feed, to improve immunity, to reduce mortality, to reduce odor, and to promote compost composting. In addition, there are disadvantages of producing only some enzymes, not various kinds.

(0001) Patent No. 1380738 (0002) Patent No. 1085832 (0003) Patent No. 0380192 (0004) Patent No. 100382 (0005) Patent No. 134562 (0006) Patent No. 165955

Bacillus subtilis, which has excellent decomposition ability of organic matter, is cultivated and adsorbed on a by-product of grains such as liquid, freeze-dried or degreasing steel, rice bran or soybean meal, dried and the resulting product is treated with an enzyme fermentation agent, It is used as fermented feed preparation, manure fermentation, soil improvement agent, herbal medicines fermentation agent and water quality improvement agent to rapidly decompose organic matter, shorten fermentation time and spread it on soil, improve soil, increase yield, suppress harmful bacteria, It is an object of the present invention to provide an excellent microorganism preparation which improves the quality of water and improves the odor when added to wastewater, such as growth promotion, reduction of odor of feces, reduction of flies, reduction of occurrence of diseases due to increase of immunity, and the like.

According to an aspect of the present invention,

Bacillus subtilis sw002 (accession number: KACC92034P), which is excellent in the ability to produce insoluble phospholipase and has antibacterial activity against Staphylococcus aureus.

In addition, the present invention relates to the aforementioned Bacillus subtilis The present invention also provides an enzyme fermentation agent containing subtilis sw002 as an active ingredient, a microorganism for processing manure, a microorganism for livestock feed, a microorganism for accelerating crop growth, a microorganism for producing fermentation broth, a water quality improving agent and a feed additive for fish.

Hereinafter, the present invention will be described in detail.

The present invention relates to a bacterium belonging to the genus Bacillus subtilis (Bacillus subtilis) having excellent antibacterial activity against E. coli, Salmonella and Staphylococcus aureus, having excellent activity of protein, starch, fibrin, phosphorus and lipolytic enzymes, Bacillus subtilis ) sw002 [accession number: KACC92034P]. Bacillus subtilis sw002 (accession number: KACC92034P) of the present invention was deposited on January 29, 2015 with the microorganism deposit number KACC92034P at the National Institute of Agricultural Science and Technology.

Of the present invention, Bacillus subtilis (Bacillus subtilis ) sw002 [Accession number: KACC92034P] is as follows.

1) Types of bacteria

When cultured in LB agar plate medium at 37 ° C for 2 days,

Gram stain: positive

Spores: Yes

Cell morphology: Bacillus

Motility: Positive

2) Physiological properties

Growth temperature: 10 ~ 55 ℃

Optimum growth temperature: 25 ~ 45 ℃

Growth possible pH: 3.0 ~ 8.5

Optimum pH: 5.0 to 7.5

Influence on oxygen: Tonic anaerobic

The present invention relates to the use of Bacillus subtilis When the fermentation broth was used as an enzyme fermenting agent, a livestock feed additive, a fermented feed preparation, a manure fermentation, a soil improvement agent, an herbicide fermentation agent and a water quality improver, the fermentation time was shortened, metabolism products and cell mass were increased, Improvement of feed efficiency, improvement of weight gain, reduction of fecal matter by improvement of digestibility, improvement of meat quality, improvement of meat quality, improvement of water quality, improvement of water quality, promotion of manure fermentation, promotion of fermentation, reduction of mortality, reduction of odor, have.

The present invention also relates to a method for producing a lyophilized product, which comprises culturing Bacillus subtilis sw002 (accession number: KACC92034P) having excellent decomposition ability for organic substances and using it alone or in combination with yeast, actinomycetes, photosynthetic bacteria, lactic acid bacteria, Or when they are adsorbed on the byproducts of grain processing such as degreasing steel, soybean meal, rice bran, etc., the effect can be maximized.

Fig. 1 is a photograph of seedlings of Example 1 and seedlings sprayed with no additive in a nursery. Fig.

Hereinafter, the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited to the following Examples.

[Example 1]

First, Bacillus subtilis Subtilis sw002 (KACC92034P ) was prepared by adding glucose (26 g), molasses (5 g), yeast extract (10 g), soy peptone (5 g ) and sodium chloride (2 g) in 1 liter of water to pH 6.5 and sterilized at 121 ° C for 15 minutes. The sterilized medium was cooled to 30 DEG C, and the seed culture was inoculated at about 1% in the same medium at 37 DEG C for 24 hours, and cultured in a fermenter at 37 DEG C, 200 rpm, and 1 vvm. The number of Bacillus subtilis sw002 (KACC92034P) obtained at this time was 10 ⁸ cfu (colony forming unit) per ml.

Bacillus subtilis subtilis) sw002 (KACC92034P) bacteria promote carbohydrate, protein, fat, food, feed, digestion and absorption rapidly decompose organic matter in the soil, as well as to act to decompose the cellulose, such as a type of Bacillus bacterium (Bacillus subtilis) and an enzyme and Amino acids, vitamins, minerals, and UGF to promote the metabolism of human and livestock, and improve the soil quality.

As described above, Bacillus subtilis subtilis) sw002 (KACC92034P) After culturing the bacteria, freeze-dried or liquid culture medium for degreasing steel, rice bran, soybean meal, wheat blood, maekgang, grain processing by-products such as rice hulls 1 were mixed in a liquid culture medium 0.5 to 1 weight ratio of 45 ℃ And dried until the moisture content in the drier became 10% or less.

[Experimental Example 1] Enzyme activity measurement

Of Example 1 Bacillus subtilis (Bacillus subtilis) sw002 (KACC92034P) creating a crude enzyme solution to the culture was diluted 10 times, and a commercially available A company, B company, C company, D Company and E four Bacillus subtilis products in the culture it contained each in The enzyme activity was measured by determining the extent of substrate degradation using a medium containing each substrate for each enzyme as follows.

Soluble starch, skim milk, and carboxylates were tested for the presence of protease, amylase, cellulase, ability phosphate solubilizing, and lipase in selected strains. Activity of enzyme was measured according to the degree of clear zone formation in agar medium supplemented with Methyl Cellulose, Sodium phytate, Tributyrin, and Ca3 (PO4) 2.

Enzyme activity measurement protein
Lytic enzyme Starch
Lytic enzyme fibrin
Lytic enzyme Insoluble phosphoric acid
Lytic enzyme Fat
Lytic enzyme Example 1
+++ +++ +++ ++ ++ Company A
Bacillus Subtilis + ++ + - - Company B
Bacillus Subtilis + + - - - Company C
Bacillus Subtilis ++ ++ - - - Company D
Bacillus Subtilis + + - + + Company E
Bacillus Subtilis + + + - +

(-: no activity, +; active, ++: active superior, +++: very active)

As a result of the measurement of the enzyme activity, the activity of the Bacillus subtilis products of the A to E companies was low in the protein and the starch and the activity was little or no in the case of the fibrin, the insoluble phosphoric acid and the fat as shown in Table 1, 1 of Bacillus subtilis subtilis) sw002 (KACC92034P) cultures protein, was measured to have excellent activity for the starch and cellulose, it has also been determined to have excellent activity even for water-insoluble phosphate, and fat.

[Experimental Example 2] Acid resistance and bile acid resistance test

Of Example 1 Bacillus subtilis (Bacillus subtilis) sw002 (KACC92034P) were cultured as for the order to determine the acid resistance and bile acid of the bacteria, in the case of the acid resistance test was inoculated to the culture of Bacillus subtilis TS Broth 1% 24 hours at 37 ℃ aerobic, the culture medium And treated with 10% HCl at pH 2 for 2 hours. And then the ratio of the number of viable cells after the pH 2 treatment to the number of the initial viable cells. In the case of the bile acid test, 1.0 wt% bile (bile) was added to the Bacillus subtilis strain, and the growth was confirmed.

As a comparative group, Bacillus subtilis ATCC 6633, Bacillus subtilis ATCC 33068, Bacillus subtilis ATCC 9372 and Bacillus subtilis KCTC 1331 were treated under the same conditions, and the results are shown in Table 2 below.

Results of acid resistance and bile acid test Strain My bile acid Acid resistance (%) Example 1 > 1.0% 100 Bacillus subtilis ATCC 6633 > 1.0% 75 Bacillus subtilis ATCC 33068 > 1.0% 60 Bacillus subtilis ATCC 9372 > 1.0% 15 Bacillus subtilis KCTC 1331 > 1.0% 85

As shown in Table 2, it was confirmed that the bile acid tolerance was improved when 1.0 wt% bile was added to all of the conventional Bacillus subtilis ATCC 6633 as well as Example 1.

However, with respect to the acid resistance, it was confirmed that the number of viable cells in Example 1 and the strain of Bacillus subtilis KCTC 1331 did not significantly change even after the pH 2 treatment. In particular, in Example 1, 100% was excellent in acid resistance. However, Bacillus subtilis ATCC 9372 was found to be vulnerable to acidic conditions.

That is, the exemplary Bacillus subtilis (Bacillus of Example 1 subtilis ) sw002 (KACC92034P) was superior to other conventional Bacillus species in bile acid and acid tolerance.

[Experimental Example 3] Evaluation of antibacterial activity against harmful bacteria

Of Example 1 Bacillus subtilis (Bacillus subtilis a) sw002 (KACC92034P) for measuring antibacterial activity of the bacteria Escherichia coli (E. coli), Salmonella (Salmonella typhimurium), Staphylococcus aureus (Staphylococcus aureus) were each liquid culture. LB agar medium was prepared in 3 plats and sterilized at 121 ° C for 1.5 minutes under high pressure at 121 ° C. After cooling to 40 ° C in a clean bench, the culture broth of Escherichia coli, Salmonella and Staphylococcus aureus After shaking, 100 μl of the solution is shaken, and the solution is dispensed into a sterilized Petri dish to produce a test strain of Escherichia coli, Salmonella, and Staphylococcus aureus.

Of Example 1 Bacillus subtilis (Bacillus subtilis) sw002 (KACC92034P) bacteria and, in Experimental Example 1 was diluted 100-fold the products purchased from the Clear to zone occurrence loads by 100㎕ in Paper disc incorporated with a test strain cultured on Plate Incubator to 37 ℃ for 24 hours and formed And the results are shown in Table 3.

Results of antibacterial experiments Escherichia coli
Cree Zone (mm) Salmonella
Cree Zone (mm) Staphylococcus aureus
Cree Zone (mm) Example 1 8 7 8 A company Bacillus subtilis 2 4 One B Company Bacillus Subtilis One 2 3 C Bacillus Subtilis 4 5 3 D Bacillus Subtilis One 2 4 E Bacillus Subtilis One One One

Of Example 1 as shown in Table 3 Bacillus subtilis (Bacillus subtilis ) sw002 (KACC92034P) was superior to other Bacillus subtilis strains by having more than 7mm of crorial zone.

[Experimental Example 4] Measurement of decomposition and fermentation state of plum

The bacillus subtilis of Example 1 ( Bacillus sp. The decomposition and fermentation state of plum was measured by comparing the mussel enzyme prepared by adding 0.1 wt% of cultured subtilis sw002 (KACC92034P ) and the mussel enzyme prepared without addition.

Of plum enzyme Example 1 Bacillus subtilis (Bacillus subtilis) and sw002 (KACC92034P) is added without additives sphere sphere divided, each at 25 ℃ 30 ilgan storage after recovery was confirmed that the fermentation conditions and the results are shown in Table 4.

Results of decomposition and fermentation of plum
30 days past Plum condition Undiluted solution
No additives

Keep plum shape
Brown
Shu Furniture

Does not maintain plum shape
Dark brown

As shown in Table 4, the addition of 0.1 wt% of the culture of Bacillus subtilis sw002 (KACC92034P) of Example 1 improved the decomposition of plums and the concentration of plums due to fermentation I confirmed that it was coming out much darker.

[Experimental Example 5] Ammonia gas inhibiting effect measurement

Of Example 1 Bacillus subtilis (Bacillus subtilis) was measured sw002 (KACC92034P) was added to the culture swine, 0.1wt% to manure, pig manure without addition, compared to the manure ammonia gas inhibitory effect. Ammonia gas concentration was measured at 35 ° C, 10 days, 30 days, and 90 days after each experiment group using a detector tube. The results are shown in Table 5.

Ammonia gas inhibition effect measurement result division 10 days past 20 days past 30 days past Stairway
0.1wt% added 8 ppm 3 ppm 0 ppm Not added 32ppm 55ppm 47ppm Swine
0.1wt% added 10ppm 4 ppm 0 ppm Not added 55ppm 79 ppm 45ppm

As shown in Table 5, when the culture of Bacillus subtilis sw002 (KACC92034P) of Example 1 was added in an amount of 0.1 wt%, it was confirmed that the ammonia concentration was remarkably decreased in both pork and paddy fields.

[Experimental Example 6] Measurement of broiler churn rate

The broilers 12,000 rats each divided into two groups, one group (impregnated furniture), the first embodiment of the Bacillus subtilis (Bacillus subtilis) sw002 (KACC92034P) administering about 0.1wt% and mix the culture in the diet, and another group (no addition old), after administration of only a conventional feed compared to shipping rate, body weight gain, feed intake and feed conversion, and ammonia generation amount , The amount of flies produced, and the state of growth. The results are shown in Table 6.

Measurement results such as broiler churn rate division Shu Furniture No additives Remarks Number of inputs (horses) 12,000 12,000

×: No occurrence
Δ: Slight occurrence
○: many occur Number of shipment (horses) 11,569 11,258 Shipment rate (%) 96.4% 93.8% Date of shipment (Sun) 32 34 Average shipment weight (Kg) 1.67 1.66 Feeding rate (%) 1.65 1.72 Ammonia gas 4 ppm 21 ppm Flies △ ○

Shipping rates as shown in Table 6, body weight gain, feed intake and feed conversion, ammonia gas generation, generation Paris, Bacillus subtilis of exemplary results confirm the growth conditions, such as for example 1 (Bacillus subtilis ) sw002 (KACC92034P) cultivars were excellent in the production rate, broiler growth condition and environment. As a result, odor reduction and flies were remarkably reduced, and it was proved that the improvement of the growth promotion and the productivity were improved by improving the environment of the housing as well as the livestock.

[Experimental Example 7] Measurement of weight gain and the like of breeding pigs

Grower divides the group into two groups each of two 50 (impregnated furniture) is Example 1 of Bacillus subtilis (Bacillus Compared subtilis) sw002 (KACC92034P) administering about 0.1wt% and mix the culture to the feed and, in the other group (no addition old) after administration of only the conventional feed for weight gain, feed intake and feed conversion, ammonia and the like generated Paris And the results are shown in Table 7.

Measurement results such as the weight gain of breeding pigs division Shu Furniture No additives Remarks Disclosure 50 50

×: No occurrence
Δ: Slight occurrence
○: many occur Disclosed average weight (Kg) 18.1 18.2 Shipment Average Weight (Kg) 115.4 108.6 Weight gain (kg / day) 1.07 0.99 Feed intake (kg / day) 2.63 2.58 Feed rate 2.53 2.68 ammonia 4 ppm 15 ppm Flies △ ○

In embodiments such as the growth conditions, the growth environment, as shown in Table 7 of Example 1 Bacillus subtilis (Bacillus subtilis ) sw002 (KACC92034P) cultures were very good. Ammonia concentration and flies also decreased, improving the breeding environment.

[Experimental Example 8] Measurement of weight gain of non-steers

Bacillus subsp. ≪ RTI ID = 0.0 > Bacillus < / RTI > ( Bacillus subtilis) in Example 1 was fed to the control diet, subtilis) sw002 (KACC92034P) was measured after addition of the culture 0.1wt% weight gain, and the results are shown in Table 8.

The result of the measurement division Starting weight (kg) Finished weight (kg) Weight gain (kg) Control 353 675 322 Test section 349 724 375

It conducted compared to the control as shown in Table 8 of Example 1 Bacillus subtilis (Bacillus The results are summarized as follows: (1) The weight gain of the test group supplemented with subtilis sw002 (KACC92034P) culture was significantly improved compared with the control group.

[Experimental Example 9] Measurement of flow rate and the like of a cow

Intended for cows 80 both one month and four months of Bacillus subtilis (Bacillus of Example 1 to an existing feeding, 2 months and 3 months old Feed subtilis) sw002 (KACC92034P) culture of a 0.1wt% benefit after flow, milk fat, milk protein, can be somatic, we investigated the disease outbreaks, The results are shown in Table 9.

Measurement results such as flow rate of cow division Flow rate (kg) butterfat(%) Milk protein (%) Somatic cell count (million) Illness outbreak Control 29.3 3.83 3.17 22 0 Test section 31.2 3.91 3.23 15 0

As shown in Table 9, milk, milk fat and milk protein were increased in the test group compared with the control group, and the number of somatic cells and the occurrence of diseases were reduced, and the milk grade and quality were improved.

[Experimental Example 10] Measurement of meat quality grade and meat quality of Hanwoo steer

Castration beef feedlot finishing from about one year to the mid late TM Bacillus according to the first embodiment seen in the feed subtilis (Bacillus Subtilis ) sw002 (KACC92034P) Cultures were added with 91 wt% of the culture, 91 of them were added and 91 of them were not added. The results of measurement of meat quality were shown in Table 10, and the results of measurement of meat quality were shown in Table 11 .

Meat quality grade of Hanwoo steers Rating 1 ++ 1+ One 2 3 A B C Shu Furniture
(%) 34.1 30.8 23.1 12.1 - 58.2 33.0 8.8 No additives
(%) 16.3 33.3 34.5 14.9 1.1 26.8 44.9 28.2

Measurement results of meat grade of Hanwoo steers Conductor weight
(kg) Backfill thickness
(mm) Sirloin cross-sectional area
(cm2) Meat index Intramuscular fat map
(No) Shu Furniture 451 12.9 90.65 64.8 6.25 No additives 425 16.34 89.9 62.14 5.4

As shown in the above Table 10 and Table 11, the addition level of the addition group was significantly improved compared to the non-addition level.

[Example 11] Measurement of digestibility of beef cattle

For cattle 10 rats each divided into two groups, one group (impregnated furniture), the first embodiment of the Bacillus subtilis (Bacillus subtilis) sw002 (KACC92034P) administering about 0.1wt% and mix the culture in the diet, and another group (no addition old) is measured bunbyeonryang to measure the digestibility of about 10 days excretion after 5 days of administration of only a conventional feed And the results are shown in Table 12.

Results of digestibility measurement of cattle division Fecal matter No additives 351.5kg Shu Furniture 246.1 kg

As shown in Table 12 were reduced, the amount of slurry of 30% in the impregnated furniture that of Example 1 Bacillus subtilis (Bacillus It is assumed as a result of the hydrogenation rate subtilis) sw002 (KACC92034P) feed by the culture higher.

[Example 12] Bacillus subtilis culture comparative test

Of Example 1 Bacillus subtilis (Bacillus subtilis) sw002 (KACC92034P) culture medium and separating the company A to E's Bacillus subtilis used in the commercially available full dissolution is possible medium LB medium and, at 37 ℃ using an 'S Bacillus culture media that are available on the market After shaking culture at 200 rpm for 48 hours, the supernatant was discarded by centrifugation at 10,000 rpm, and the amount of the remaining amount was measured to compare the amounts of metabolites and cells. The results are shown in Table 13.

Comparison test results of Bacillus subtilis culture LB medium S Company Bacillus culture medium Example 1 1.54 g 2.33 g A company Bacillus subtilis 1.39 g 1.31 g B Company Bacillus Subtilis 1.59 g 1.54 g C Bacillus Subtilis 1.42g 1.45 g D Bacillus Subtilis 1.41 g 1.28 g E Bacillus Subtilis 1.37g 1.79 g

As shown in Table 13, the culture medium of Bacillus subtilis sw002 (KACC92034P) of Example 1 showed 1.5 times higher metabolic products and cell masses than the other Bacillus subtilis.

[Example 13] Measurement of germination rate and growth rate of hair growth

Of Example 1 Bacillus subtilis (Bacillus subtilis ) sw002 (KACC92034P) culture was diluted 1,000 times and sprayed on the rice paddy field, and the growth power was compared with that of the non-sprayed hair growth. The results of the test are shown in Fig.

As shown in Fig. 1, the germination rate and growth rate were higher than those of the non-additive group.

[Example 14] Comparison test of fermentation of manure with manure

Of Example 1 Bacillus subtilis (Bacillus subtilis) after the sw002 (KACC92034P) a culture aeration charged into a 200-liter fermentation tank of money 400 ton manure were measured manure fermentation effects, The results are shown in Table 14.

Comparison of fermentation effluent from manure division Completion period No additives 45 days A Company Bacillus Subtilis Products
0.05% added 42 days The Bacillus subtilis culture solution of Example 1
0.05% added 36 days

As shown in Table 14, the cultivation solution of Bacillus subtilis sw002 (KACC92034P) of Example 1 was shortened by 6 to 9 days in terms of the duration of the liquid pot for the non-additive group and the Bacillus subtilis product of the A group.

[Example 15] Waste water treatment comparative test

Of Example 1 Bacillus subtilis (Bacillus subtilis) sw002 (KACC92034P) after 50ppm added daily to cultures in broiler processing plant waste water treatment of the effluent water was measured, The results are shown in Table 15.

Comparative test results of wastewater treatment Test Items Emission Standards Drain before addition Drain after addition pH 5.8-8.6 6.9 7.1 COD (mg / L) 90 or less 85.7 25.4 BOD (mg / L) Below 80 60.2 12.5 SS (mg / L) Below 80 36.6 7.0 n-H (optical oil) (mg / L) 5 or less 3.3 0.4 n-H (copper, plant) (mg / L) 30 or less 15.4 1.0 T-N (mg / L) 60 or less 43.238 1.788 T-P (mg / L) 8 or less 7.351 5.496 Chromaticity (degrees) 300 or less 175.5 20.1

[Example 16] Comparative test of two sheets

Of Example 1 Bacillus subtilis (Bacillus Subtilis ) sw002 (KACC92034P) culture medium was added at an interval of 5 days to the exponential growth rate of 20 ppm per day to examine the activity and water quality of the eel. The results are shown in Table 16.

Comparison of test results division Chilling mortality Eel activity Water quality No additives usually usually Bad A Company Bacillus Subtilis Products
20ppm addition Reduction Active usually Example 1 Bacillus subtilis culture solution
20ppm addition Much saved Active Good

As shown in Table 16, when the culture medium of Bacillus subtilis sw002 (KACC92034P) of Example 1 was added, the activity of the eel was significantly superior to that of the non-supplemented group, and the pear mortality was also low.

[Example 17] Foliar fertilization such as bell pepper

Of Example 1 Bacillus subtilis (Bacillus subtilis ) sw002 (KACC92034P) cultures were diluted 500 times, and then foliar application and control were applied to bell pepper, cucumber red pepper, strawberry, and branches. As a result, the following phenomenon was observed.

The green pepper has a broad leaf and a thick side face. The leaf has a shiny color as it approaches the initial level, the fresh color begins to appear, the rind is slightly thick, and the green pepper is slightly longer and shiny.

And the cucumber pepper has wider leaf, thicker thickness and more leaf color, and the occurrence of gingival and anomalies is remarkably reduced.

Strawberries were bloomed, sugar content was improved to 2 ~ 3 brix, the color of leaves and strawberries became thicker, the leaves became thicker, and the rooting of the roots became better.

The cucumbers were large in number of flowers, increased in yield, and longer in size.

Claims (8)

Bacillus subtilis sw002 (Accession number: KACC92034P), which has excellent ability to produce insoluble phosphatase and antibiotic resistance against Staphylococcus aureus.
The method of claim 1, wherein the Bacillus subtilis subtilis ) sw002 as an active ingredient.
The method of claim 1, wherein the Bacillus subtilis subtilis ) sw002 as an active ingredient.
The method of claim 1, wherein the Bacillus subtilis Subtilis ) sw002 as an active ingredient.
The method of claim 1, wherein the Bacillus subtilis subtilis ) sw002 as an active ingredient.
The method of claim 1, wherein the Bacillus subtilis subtilis ) sw002 as an active ingredient.
The method of claim 1, wherein the Bacillus subtilis subtilis ) sw002 as an active ingredient.
The method of claim 1, wherein the Bacillus subtilis subtilis ) sw002 as an active ingredient.

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