KR101548340B1 - Hangover recovery drink using bean sprouts and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a hangover-free beverage using bean sprouts and a method for producing the same, and more particularly, to a bean sprout cultivated with water having a high concentration of dissolved oxygen, which contains a combination extract of Hovenia dulcis And to a process for producing the same.
The hangover-eliminating beverage using the bean sprouts of the present invention is characterized in that the bean sprouts extract contains a complex extract obtained by extracting a mixture of hovenous fruit and pine needle.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a hangover recovery drink using bean sprouts,

The present invention relates to a hangover-eliminating beverage using bean sprouts and a method for producing the same, and more particularly, to a bean sprout cultivated with water having a high concentration of dissolved oxygen, which contains a combination extract of Hovenia dulcis And to a process for producing the same.

Alcohol suppresses the central pace of the brain, and when it is paralyzed, it becomes apparently excited. It is a modern food which is stressful because it suppresses consciousness and movement and has sleep and anesthetic effect. Along with economic growth, drinking culture in our country is not only a part of social activities for economic activities, but also a part of cultural life to alleviate mental loneliness from modern society.

As such, alcohol intake is good for diversion and blood circulation can be beneficial to health, but excessive intake of chronic intake of alcoholic liver disease and other health problems, can be developed to addiction, The central nervous system such as the brain, as well as the digestive organs and metabolic organs such as liver can damage the health of the individual is becoming a serious problem. In addition, the risk of traffic accidents resulting from drinking is becoming a social problem apart from individual problems, and the demand for foods and medicines that can minimize the impact of alcohol on the human body is also increasing.

When you drink alcohol, alcohol is absorbed in the esophagus in a small amount, about 10% in the stomach, 90% in the small intestine, absorbed alcohol flows along the bloodstream to the brain, liver and other tissues of the body, about 10% And the remaining 90% is degraded in the liver.

The first step in alcohol degradation is the conversion of alcohol dehydrogenase (ADH) to alcohol by oxidation to acetaldehyde, which in turn is oxidized by acetaldehyde dehydrogenase (ALDH) and converted to acetic acid, which is harmless to the body . This acetic acid is decomposed again into water and carbon dioxide and discharged to the outside of the body. At each step, one molecule of reduced NADH is produced from oxidized NAD +, where NAD + acts as a coenzyme. Alcohol metabolism by alcohol dehydrogenase in the liver produces NADH as a by-product and increases the NADH / NAD + ratio. This change in redox state affects several intermediate levels of metabolism.

In particular, lactic acid should use NAD + for conversion to pyruvic acid, but since NAD + is used for alcohol oxidation, lactic acid is not converted to pyruvic acid and accumulates to lower the pH of the blood. In addition, although the NADH / NAD + ratio increases during alcohol oxidation, it is necessary to maintain these ratios normally for the continuous oxidation of alcohol. Thus, pyruvic acid is converted to lactic acid by oxalacetic acid in the TCA circuit to lactic acid, and NAD + produced at this time is used. These deficiencies of pyruvic acid and oxaloacetic acid may interfere with the glucose neogenesis process, leading to poor glucose synthesis, which can lead to hypoglycemia when fasting alcohol is consumed.

The symptoms of hangover after alcohol consumption are known to cause discomfort, headache, elevated blood pressure, flushing, and vomiting as acetaldehyde, a byproduct of alcohol decomposition, does not decompose.

Acetaldehyde's long stay in the cerebrum and liver can lead to damage to the nervous system and serious liver disease. Cerebral atrophy, cerebral atrophy, alcoholic retinopathy, alcoholism, anxiety, irritability, depression, sleep disorders, alcoholic dementia, It causes serious physical problems such as hallucination, hallucination, and so on.

Drugs that can reduce such hangover symptoms have been actively studied and many products have already been introduced. There are various kinds of drugs for hangovers that are marketed in Korea such as Condition (Cheil Jedang), Aspa (Target), Solpyo Business (Korean shipbuilding), Daehan 808 (Grammy), Reception (Future Bio), Morning Care .

A number of hangover-free beverages using a bean sprouts extract as a patented technology have been disclosed.

Korean Patent Laid-Open No. 2004-0005545 discloses a method for producing hangover-free beverages, and Japanese Patent Laid-Open Publication No. 2006-0087323 discloses a method for producing bean sprouts beverage based on bean sprouts. In the patent No. 0989869, A hangover-eliminating beverage containing a fermentation liquid is disclosed.

However, since the bean sprouts cultivated by the conventional cultivation method are used for the hangover-eliminating beverage, there is a problem that the hangover resolution effect is not high.

The present invention has been made in order to overcome the above problems, and it is an object of the present invention to provide a bean sprout which is grown with water having a high concentration of dissolved oxygen and a combination extract of Hovenia dulcis And a manufacturing method thereof.

In order to achieve the above object, the hangover-eliminating beverage using the bean sprouts according to the present invention is characterized in that the bean sprouts extract contains a complex extract derived from a mixture of Hovenia dulcis var.

The bean sprouts extract is characterized in that bean sprouts are mixed with oligosaccharides and then laid for 1 to 3 days, followed by removal of bean sprouts.

The bean sprouts are cultivated by adjusting the dissolved oxygen concentration of the cultivated water to 10 to 20 ppm.

The bean sprouts are green bean sprouts whose heads are green.

To achieve the above object, there is provided a method for producing a hangover-free beverage using bean sprouts according to the present invention, comprising: culturing bean sprouts by cultivating soybeans and supplying cultivation water; A first extraction step of extracting a bean sprouts extract from the bean sprouts; Mixing the bean sprouts extract with hornblende and pine leaves to obtain a mixture; A second extraction step of extracting the complex extract by adding an extraction solvent to the mixture; And a step of diluting the complex extract with water.

The cultivation step is characterized in that the cultivated water having the dissolved oxygen concentration adjusted to 10 to 20 ppm is sprinkled for 5 to 15 minutes every 2 to 6 hours.

Wherein the cultivation step comprises: a) placing the germinated soybeans in a cultivation vessel, cultivating the cultivated water in a dark room at 20 to 30 캜 for 5 to 15 minutes at intervals of 2 to 6 hours, and then primary culturing for 5 to 7 days; and b) And then cultivating the bean sprouts firstly cultivated with red light for 2 to 4 days.

As described above, the present invention contains a combined extract of bean sprouts, hut fruit and pine leaves, and is excellent in liver protection and hangover relieving effect, and thus can be usefully used as a hangover-free beverage.

And the use of bean sprouts extract extracted from bean sprouts grown with water with a higher concentration of dissolved oxygen can further improve the effect of preventing liver and hangover.

1 and 2 are graphs showing the survival rate of HepG2 cells by hydrogen peroxide treatment.

Hereinafter, a hangover-eliminating beverage using bean sprouts according to a preferred embodiment of the present invention and a method of producing the hangover-free beverage will be described in detail.

The hangover-eliminating beverage according to one embodiment of the present invention contains a complex extract. The compound extract is extracted from the mixture of the hornblende and the pine needle with the bean sprouts extract. For example, the mixture may be prepared by mixing 0.1 to 20 parts by weight of Hovenia dulcis and 0.1 to 20 parts by weight of pine needle per 100 parts by weight of the bean sprouts extract. The resulting mixture is extracted with an extraction solvent.

Hovenia fruit and pine needles are added with bean sprouts extract to improve the hangover effect of beverages and enhance nutritional components.

In the present invention, the bean sprouts extract can be extracted by various methods. Extraction solvent can be added to bean sprouts by a conventional method. As the extraction solvent, at least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixed solvent thereof may be used. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. As the mixed solvent, a mixture of water and a lower alcohol, a mixture of water and a polyhydric alcohol, a mixture of a lower alcohol and a polyhydric alcohol, or a mixture of water and a lower alcohol and a polyhydric alcohol can be used.

As an example of the bean sprouts extract, extracting the bean sprouts with hot water extraction, cold extraction or warm-up extraction at 10 to 150 ° C for 1 to 24 hours after adding 2 to 20 times the weight of the extraction solvent, and filtering the bean sprouts.

Unlike the conventional extraction method, soybean sprout extract can be extracted using oligosaccharide. For example, 100 parts of bean sprouts may be mixed with 100-300 parts of oligosaccharide, and the bean sprouts may be obtained by removing only the bean sprouts from the mixture at room temperature of 15 to 25 DEG C for 1 to 3 days. Here, the bean sprouts extract is an oligosaccharide from which bean sprouts are removed. When bean sprouts are placed on oligosaccharides, the usefulness of bean sprouts and moisture are released into oligosaccharides. When bean sprouts are extracted using oligosaccharides, the bean sprouts useful in bean sprouts can be easily extracted even at low temperatures, and the proper sugar content of the beverage can be maintained without adding any additional sweetener.

The bean sprouts used as the main material may be bean sprouts grown by a conventional method, but bean sprouts cultivated by supplying cultured water with a dissolved oxygen concentration of preferably 10 to 20 ppm are preferably used. Soybean sprouts grown with cultivated water with a higher concentration of dissolved oxygen than conventional ground water or water can improve the hangover resolution effect.

In addition, bean sprouts can use green bean sprouts whose heads are green. It is possible to enhance the functionality of the green bean sprouts hangover drink.

The hangover-eliminating beverage of the present invention contains 20 to 90% by weight of the combined extract in the total weight of the beverage. For example, the hangover-resolved beverage may be composed of 20 to 90% by weight of the combined extract and 10 to 80% by weight of water.

 It is needless to say that the hangover-free beverage of the present invention may contain known additives which are pharmaceutically acceptable. Examples of additives include taurine, citric acid, vitamin C, and carbohydrates. The carbohydrate may be a monosaccharide such as glucose, fructose, etc .; Disaccharides such as maltose, sucrose and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and xylitol, sorbitol, erythritol, and the like. In addition, various flavorings such as ordinary beverages may be contained as additional ingredients. Natural flavoring agents such as tau martin and stevia extract can be used as flavorings.

Hereinafter, a method for producing a hangover-free beverage using the bean sprouts according to the present invention will be described.

A method for producing a hangover-resolved beverage according to the present invention comprises: a cultivation step of cultivating soybean sprouts by supplying cultivated water; a first extracting step of mixing soybean sprouts and oligosaccharides cultivated and removing soybean sprouts to obtain soybean sprouts; A second extraction step of extracting the complex extract by adding an extraction solvent to the mixture, and a step of diluting the complex extract by adding water to the mixture. We will look at each step in detail.

1. Growth stage

First, soybean is germinated and cultivated with soybean sprouts. For example, beans are soaked in water for one day and then germinated by standing at 20 to 30 ° C. Then, the germinated soybeans are placed in a conventional bean sprout cultivation vessel, cultivated in a dark room at 20 to 30 ° C for a period of 5 to 7 days. Cultivation water can be sprayed every 2 to 6 hours at 5 to 15 minutes.

Preferably, the cultivated water has a dissolved oxygen concentration adjusted to 10 to 20 ppm. It is made by cultivating water by artificially dissolving oxygen in ground water or water and adjusting the concentration of dissolved oxygen to 10-20 ppm.

The concentration of dissolved oxygen in the cultivation water used in the present invention is 10 to 20 ppm, whereas the amount of dissolved oxygen in the groundwater or the constant used in conventional bean sprouts cultivation is 5 to 7 ppm. When soybean sprouts are grown by increasing the concentration of dissolved oxygen in the cultivated water, the content of aspartic acid contained in the soybean sprouts can be increased and the hangover resolution effect can be improved.

Meanwhile, the cultivation step according to another embodiment of the present invention can be divided into a first cultivation without light irradiation, and a second cultivation with light irradiation.

The primary cultivation is the same as the cultivation method described above. That is, the germinated soybeans are placed in a cultivation vessel, cultivated in a dark room at 20 to 30 ° C for 5 to 15 minutes at intervals of 2 to 6 hours, and cultivated for 5 to 7 days. Conventional ground water or a constant water may be used as the cultivation water in the first cultivation, but it is preferable to use a solution in which oxygen is artificially dissolved to adjust the concentration of dissolved oxygen to 10 to 20 ppm.

In the second cultivation, bean sprouts are cultivated for the first time, and light is irradiated to allow the bean sprouts to perform photosynthesis. The second cultivation is the same as the first cultivation and cultivation except for cultivation period and light irradiation. For example, in the second cultivation, soybean sprouts are grown for 2 to 4 days by irradiating the dark room with red light. A bean sprout is grown by installing a light source that emits red light of 600 ~ 700nm wavelength in the dark room.

Through the second cultivation, the bean sprouts are changed from yellow to green, and have various nutritional components which do not have yellow bean sprouts. Green bean sprouts with green hair are rich in various nutrients, which can enhance the functionality of hangover-free beverages.

2. First extraction step

Bean sprouts are grown and harvested. Wash the harvested bean sprouts thoroughly with water and remove the water.

The bean sprouts extract can be extracted by various methods. Extraction solvent can be added to bean sprouts by a conventional method. As the extraction solvent, at least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixed solvent thereof may be used. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. As the mixed solvent, a mixture of water and a lower alcohol, a mixture of water and a polyhydric alcohol, a mixture of a lower alcohol and a polyhydric alcohol, or a mixture of water and a lower alcohol and a polyhydric alcohol can be used.

As another example of the method of extracting bean sprouts, the prepared bean sprouts are mixed with oligosaccharides and then put on for 1 to 3 days. 100 parts of soybean sprouts 100 to 300 parts of oligosaccharide per skin When the skin is mixed and then stored at room temperature of 15 to 25 ° C for 1 to 3 days, the components of soybean sprouts and moisture are discharged to oligosaccharides. The bean sprouts are mixed with the oligosaccharides and allowed to stand for 1 to 3 days. Only the bean sprouts are extracted to obtain the bean sprouts extract.

3. Mixing step

Next, to the bean sprouts extract, a mixture of Hovenous Fruit and pine leaves is obtained. For example, 0.1 to 20 parts by weight of Hovenia dulcis and 0.1 to 20 parts by weight of pine needle can be mixed with 100 parts by weight of the bean sprouts extract.

4. Second extraction step

Extracts are extracted from a mixture of bean sprouts, hornbills and pine leaves.

For example, the extraction solvent may be extracted 2 to 20 times by weight of the extraction solvent at a temperature of 10 to 150 ° C for 1 to 24 hours using hot water extraction, cold-water extraction or warm-up extraction, followed by filtration to obtain a complex extract. In addition, a reflux cooling extraction, an ultrasonic extraction method, a supercritical fluid extraction method, or the like can be used. In addition to the above-described extraction method, an extract obtained through a conventional purification process is also included. For example, an active fraction obtained through various purification methods, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies, etc., is also included in the extract.

As the extraction solvent, at least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixed solvent thereof may be used. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. As the mixed solvent, a mixture of water and a lower alcohol, a mixture of water and a polyhydric alcohol, a mixture of a lower alcohol and a polyhydric alcohol, or a mixture of water and a lower alcohol and a polyhydric alcohol can be used.

5. Formulation step

The hangover-free beverage of the present invention is prepared by adding water to the complex extract in the formulating step and diluting it. The hangover-eliminating beverage of the present invention contains 20 to 90 wt% of the combined extract in the total weight of the beverage. For example, the hangover-free beverage may be prepared by mixing 20 to 90% by weight of the combined extract and 10 to 80% by weight of water in the formulation step.

In addition, it is a matter of course that known additives which are pharmaceutically acceptable in the formulating step can be added. Examples of additives include taurine, citric acid, vitamin C, and carbohydrates.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

<Preparation of bean sprouts extract>

Soybean sprouts were cultivated by varying dissolved oxygen concentration in cultivated water. The bean sprouts were germinated into a conventional bean sprout cultivation vessel and grown in a dark room at 25 ° C for 6 days. During the cultivation period, the cultivated water was distributed in the cultivation vessel for 10 minutes every 4 hours.

Groundwater (DOE), which has a dissolved oxygen concentration of 6ppm (hereafter referred to as general water), and oxygen water with a higher dissolved oxygen concentration were used as cultivated water. The dissolved oxygen concentration of ground water was adjusted to 10, 15, and 20 ppm using oxygen dissolver.

The bean sprouts cultivated with ground water and oxygenated water were harvested, washed thoroughly with water, and extracted with an extraction solvent. Extracts of hot water and ethanol were extracted with water and 70% ethanol.

In the case of the hot-water extract, water was added to the bean sprouts 8 times at a weight ratio, and then the mixture was extracted at 100 ° C for 6 hours and then filtered. In the case of the ethanol extract, 70% ethanol was added to the bean sprouts at a weight ratio of 10 times, and the mixture was stirred at 40 ° C and 200 rpm for 12 hours, filtered and concentrated under reduced pressure at 45 ° C for 20 minutes.

The hot and cold water extracts and ethanol extracts of soybean sprouts harvested from ordinary hot - water extracts, ordinary water ethanol extracts, and oxygenated water were added to hot water extracts and oxygenated water Ethanol extracts.

<Cell culture of bean sprouts extract according to growing conditions>

The HepG2 cells used in the experiment are cultured cells derived from human hepatocytes, and they are known to exhibit the same pattern as that of human hepatocytes for drugs and nutrients. Therefore, various studies have been conducted as model cells suitable for studying metabolism in human liver It is progressing.

Therefore, in this experiment, we investigated the effect of soybean sprout extract on the oxidative stress - induced liver injury caused by hydrogen peroxide using HepG2 cells.

1. HepG2 cell line culture

The HepG2 cell line medium consisted of 10% (v / v) fetal bovine serum, 0.5% v / v, 50 g / ml streptomycin, 50 IU / ml penicillin, 0.125 g / ml fungizone, (MEM) medium containing 5% CO ₂ and 95% humidified air at 37 ° C was used. The medium was changed every 2 days and subculture was performed when confluency was 80%.

2. Detection of Antioxidative Stress Liver Damage by Soybean Sprouts Extract

The HepG2 cell line was dispensed into a 24-well plate at 5 × 10 ⁴ cells / well. Then, a medium containing 3% of serum containing the sample was added to each well and reacted in a 37 ° C. incubator for 24 hours. After washing with PBS, the cells were treated with hydrogen peroxide. After incubation for 24 hours, the medium was removed and XTT-PMS solution was added for 2 hours. The resulting formazan was measured for absorbance at 450 nm using a microplate reader. Negative control (Cont.) Was used only media instead of sample, and positive control was treated with 0.3 mM hydrogen peroxide only.

The degree of cell damage was determined by measuring the absorbance at 450 nm for the degree of formation of formazan, setting the absorbance of the negative control added with medium instead of the sample as 100%, and calculating the relative absorbance as a cell survival rate (%) as shown in FIG. The results are shown.

(100 ppm, 300 μg / mL), normal control caffeic acid (40 μg / mL), oxygenated water (0.3 ppm) and hydrogen peroxide (100, 300 / / mL), water (0.3 mM) Hydrogen peroxide + water (100, 300 / / mL), oxygen water EtOH: 0.3 mM Hydrogen peroxide + oxygen water (15 ppm) Ethanol extract Hydrogen peroxide + normal water ethanol extract (100, 300 / / mL).

Referring to FIG. 1, the oxidative stress induced by the hydrogen peroxide treatment greatly reduced the survival rate of the cells. And it was confirmed that the cell damage caused by hydrogen peroxide was reduced by the treatment of bean sprouts extract. In particular, the oxygenated hydrothermal extract showed cell viability close to 100% as compared with the general hydrothermal extract. This result shows that the hydrothermal extracts from soybean sprouts grown by increasing the oxygen concentration have excellent protection against hepatocyte injury by hydrogen peroxide.

The hydrothermal extracts were superior to ethanol extracts in inhibiting liver damage, and further experiments were conducted using hot water extracts.

The experiment was carried out in the same manner as the above experiment. As a hot water extract, hot water extract of ordinary water and oxygenated water (10 ppm, 20 ppm) were used.

The experimental results are shown in Fig. In FIG. 2, control: negative control, H2O2: 0.3mM hydrogen peroxide, high oxygen: 0.3mM hydrogen peroxide + oxygen water (20ppm) hot water extract (100, 300g / , 300 μg / mL), hypoxic: 0.3 mM hydrogen peroxide + oxygenated water (10 ppm), hot water extract (100, 300 μg / mL)

Referring to FIG. 2, it was found that the extract of oxygenated hydrothermal solution had a somewhat protective effect against hepatocyte injury. Especially, when 300 ㎍ / mL of hot water extract extracted from soybean sprouts cultivated at 10 ppm concentration of dissolved oxygen was treated, significant difference was shown.

<Amino acid analysis>

The free amino acid content of the bean sprouts extract was analyzed using an automatic amino acid analyzer (HITACH L-8900). Standard solutions were prepared by diluting with 0.02 N HCl in 2 ml each from Wako standard amino acid Amino acid Mixture Standard Type II ANII and Type B, respectively.

For the preparation of the test solution, the same amount of sample and 16% trichloroacetic acid solution was added thereto, followed by shaking for 15 minutes, centrifugation at 3000 rpm for 15 minutes, and using the supernatant. The analytical column was used with a column for physiological Fluids Analysis # 2622 4.6 × 60 mm.

The results of amino acid analysis (unit: mg / 100 mL) are shown in Table 1 below.

Item General number
Hot water extract Oxygen water (10 ppm)
Hot water extract Oxygen water (20 ppm)
Hot water extract Phosphoserine P-ser 459.57 371.16 790.05 Taurine Tau 0.00 0.00 0.00 Urea Urea 685.90 1114.55 2277.76 Aspartic acid Asp 967.27 0.00 688.46 Threonine THR 236.08 195.54 254.54 Serine Ser 921.55 883.43 1155.50 Glutamic acid Glu 0.00 0.00 0.00 Asparagine Asp - NH4 100.77 147.02 168.23 Sarcosine SAR 0.00 0.00 0.00 alpha -amino adipic acid a-AAA 36.51 49.79 69.01 Glycine Gly 142.38 150.90 202.65 Alanine Ala 1174.94 1269.38 1864.00 Citrulline Cit 0.00 3.47 4.02 alpha -amino-n-butyric acid a-ABA 41.86 30.68 41.95 Valine Val 1565.30 1304.23 1821.14 Cystine Cys 0.00 0.00 0.00 Methionine Met 114.23 87.73 126.72 Cystathionine Cysthi 0.00 0.00 0.00 Isoleucine Ile 633.77 534.50 752.89 Leucine Leu 774.08 636.03 999.41 Tyrosine Tyr 98.88 79.30 122.12 Phenylalanine Phe 610.81 566.98 854.85 β-Alanine b-Ala 204.42 205.37 326.99 β-Amino isobutyric acid b-AiBA 36.25 136.55 182.02 gamma-amino-n-butyric acid g-ABA 350.85 321.68 352.10 Tryptophan TRP 71.31 58.95 62.78 Hydroxylysine Hylys 0.00 0.55 0.71 Ornithine Orn 0.00 12.66 37.92 Lysine Lys 350.88 329.14 506.02 1-Methylhistidine 1Mehis 15.45 28.29 38.40 Histidine His 692.85 535.70 776.41 3-Methylhistidine 3Mehis 0.00 0.00 0.00 Anserine Ans 0.00 0.00 0.00 Carnosine Car 0.00 9.19 9.73 Arginine Arg 1165.16 1470.73 2305.45 HydroProline Hypro 0.00 0.00 0.00 Proline pro 218.12 249.12 401.94 Sum 11669.20 10782.62 17193.77

Referring to the results of Table 1 above, the total amount of amino acids was higher in the extract of oxygenated water than in the extract of normal water. In particular, the content of asparagine, which is known to have a good hangover effect, was improved by about 45 to 70% as compared with that of a general hydrothermal extract.

The results of the above tests showed that soybean sprouts cultivated with 10 to 20 ppm dissolved oxygen increased the concentration of dissolved oxygen in soybean sprouts compared to soybean sprouts cultivated in conventional cultivation. Therefore, bean sprouts cultivated by conventional cultivation can be used as a hangover drink, but it is preferable to use bean sprouts cultivated with cultivated water having increased dissolved oxygen concentration in terms of functionality.

&Lt; Preparation of hangover drink >

Soybean sprouts were cultivated with the concentration of dissolved oxygen of 15ppm. 100 parts of the prepared soybean sprouts, 20 parts of oligosaccharide to the skin, mixed with the skin, allowed to stand for 2 days at 20 ° C, and then only the bean sprouts were removed to obtain a soybean sprout extract.

To 100 parts by weight of the bean sprouts extract, 5 parts by weight of Hovenous Fruit and 5 parts by weight of pine needles were mixed to prepare a mixture. Next, water was added to the mixture at a weight ratio of 10 times, followed by hot extraction at 110 ° C for 6 hours, followed by filtration to obtain a combined extract.

70% by weight of the combined extract and 30% by weight of purified water were mixed to prepare a hangover-free beverage.

<Hangover elimination experiment>

1. Test substance

The hangover-free beverage was used as the test substance. As a positive control material, a commercially available hangover beverage (Jeongmi 808, Grammy) was used.

2. Experimental animals and diets

Five-week-old male SD rats without specific pathogen free were purchased from Orient Bio (Sungnam, Korea). Experimental animals were maintained at a temperature of 23 ± 3 ℃, a relative humidity of 50 ± 10%, a ventilation frequency of 10 to 15 times / hour, and a lighting time 12 hours (08: 00 ~ 20: 00) and the illumination was set at 150 ~ 300 Lux. During the 1-week adaptation period, the experimental animals were fed free of drinking water with the solid feed for experimental animals (Cargill Agri Purina, Gunsan, Korea).

After 1 week of adaptation period, healthy animals were selected and classified into 5 groups based on the nodule method. Alcohol + hangover drink 260 mg / kg Body weight / day Oral administration group (untreated group), alcohol + hangover drink 26 mg / kg body weight / day Oral administration group (first test group) body weight / day oral administration group (second test group), alcohol + hangover elimination drink 2000 mg / kg body weight / day oral administration group (third test group), alcohol + positive control substance 41 mg / kg body weight / (Control group) and fasted for 18 hours. Body weight was measured on the day of the experiment.

Alcohol was orally administered 30 minutes after oral administration of hangover drink, positive control substance, and saline, respectively. Alcohol was diluted to 40% with 3 g / kg body weight of fermented alcohol. Blood was collected from the orbital vein at 1 hour and 3 hours after alcohol administration and blood was collected from the heart at 5 hours after the administration of alcohol. Blood was collected in a separate serum tube (Becton Dickinson, USA), left at room temperature for 30 minutes, and centrifuged at 3,000 rpm for 20 minutes to separate the serum. All animal experiments were conducted under the approval of Hallym University Animal Experimental Ethics Committee (Hallym 2013-17).

3. Measurement of serum ethanol concentration

The ethanol content in the serum was measured using the ethanol measuring kit (Abcam) according to the manufacturer's method. The serum was diluted appropriately using an Assay buffer frame, and 50 μl of the reaction mix preparation was added, and 50 μl of the reaction mix preparation was added. After incubation for 1 hour, the absorbance was measured at 570 nm. Using the standard curve prepared using the ethanol standard solution, The ethanol content was calculated.

4. Determination of serum acetaldehyde concentration

Acetaldehyde is oxidized by a liver enzyme called acetaldehyde dehydrogenase to produce acetate. In this process, NADH is produced with the aid of a coenzyme called NAD +. The amount of the product is measured at 340 nm in absorbance. The acetaldehyde measurement kit (R-Biopharm) was used according to the manufacturer's method. Reaction mixture (potassium diphosphate buffer, pH 9.0 + NAD +) was mixed with 0.2 ml of serum, incubated at 20 ° C for 3 minutes, and absorbance was measured at 340 nm (Al). After adding 0.05 ml of alcohol dehydrogenase After incubation at 20 ° C for 5 minutes, the absorbance was measured at 340 nm (A2). Then, the ethanol content in the serum was measured by the following equation.

C (g acetaldehyde / L sample solution) = (0.7258 / 6,3) x A

ΔA = Sample (A2-A1) - Blank (A2-A1)

5. Serum ALT, AST, ALP content

Serum samples isolated from the blood samples collected on the day of the autopsy were analyzed for alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) using a blood biochemical analyzer (KoneLab 20 XT, Thermo Fisher Scientific, Waltham, Finland).

6. Statistical processing

All analytical values were expressed as mean ± SEM. Statistical analysis was performed using the SAS 9.1 program (SAS Institute, Cary, NC, USA). The significance of each group was analyzed by analysis of various and Duncan's multiple range test at α = 0.05 level.

7. Experimental results

(1) Weight and liver weight of experimental animals

The results of body weight and liver weighing of the experimental animals are shown in Table 2 below.

division Weight (g) Liver weight (g) Untreated group 211.7 ± 2.6 8.2 ± 0.2 First test group 212.3 ± 2.8 8.2 ± 0.2 Second test group 212.9 ± 2.7 8.0 ± 0.1 Group 3 211.0 ± 2.3 7.9 ± 0.2 Control group 212.2 ± 2.9 7.8 ± 0.1

The mean body weight of the untreated group was 211.7 ± 2.6 g, the mean weight of the first test group was 212.3 ± 2.8 g, the mean weight of the second test group was 212.9 ± 2.7 g, and the mean weight of the third test group was 211.0 ± 2.3 g , And the control group was 212.2 ± 2.9g.

(2) Influence of concentration of ethane in blood

The ethanol concentration (g / L) after 1 hour, 3 hours and 5 hours after administration of the alcohol is shown in Table 3 below.

division After 1 hour After 3 hours After 5 hours Untreated group 1.520 + 0.045 0.703 + 0.025 0.303 ± 0.006 First test group 1.412 + 0.013 0.686 + 0.031 0.296 ± 0.006 Second test group 1.409 ± 0.009 0.700 + 0.029 0.295 ± 0.007 Group 3 1.393 + 0.016 0.664 + 0.029 0.284 ± 0.002 Control group 1.390 + 0.009 0.668 + 0.026 0.289 ± 0.004

As shown in Table 3, the ethanol concentration in the serum increased to 1.520 ± 0.045 g / L after 1 hour in the alcohol-only treatment group, and the ethanol concentration in the serum increased with the passage of 3 hours and 5 hours 0.703 ± 0.025 g / L and 0.303 ± 0.006 g / L, respectively. The ethanol concentration in the serum of the test group and the control group was significantly lower than that of the untreated group at 1 hour after the administration of alcohol, but no difference was found according to the test substance treatment capacity of the test group. The ethanol concentration in the serum of the test group after 3 hours of alcohol administration was decreased by the test substance treatment but there was no significant difference. The ethanol concentration in the test group was significantly decreased after 5 hours of alcohol administration. When the test substance was administered at a high concentration (2,000 mg / kg body weight), the ethanol concentration in the serum was 6, 3%.

(3) Influence on blood acetaldehyde concentration

The concentration (mg / L) of acetaldehyde after 1 hour, 3 hours and 5 hours after administration of the alcohol is shown in Table 4 below.

division After 1 hour After 3 hours After 5 hours Untreated group 27.02 + - 0.72 19.35 ± 1.91 33.07 ± 1.87 First test group 24.11 ± 1.00 19.44 + 0.75 31.56 + - 4.55 Second test group 25.07 ± 0.99 15.15 + 1.49 24.35 ± 2.10 Group 3 22.80 ± 1.71 10.86 ± 1.57 20.20 ± 1.27 Control group 22.85 ± 0.98 15.40 ± 0.88 25.71 ± 1.96

Referring to the results of Table 4 above, the acetaldehyde concentration in the serum of the test group and the control group was significantly lower than that of the untreated group after 1 hour of administration of the alcohol. After 3 hours of alcohol administration, the concentration of acetaldehyde in the serum of the test group (260 ㎎ / ㎏ body weight) and high concentration (2,000 ㎎ / ㎏ body weight) was decreased in the test group. After 5 hours of alcohol administration, acetaldehyde concentration in the serum was significantly decreased as the concentration of the test substance was increased. When the test substance was treated at a high concentration (2,000 mg / kg body weight), serum The acetaldehyde concentration was decreased.

(4) Effect on serum ALT, AST and ALP

ALT, AST and ALP of serum are shown in Table 5 below.

division ALT AST ALP Untreated group 60.8 ± 6.0 221.9 ± 14.3 840.8 ± 47.8 First test group 61.7 ± 4.0 236.6 ± 8.6 845.2 ± 45.4 Second test group 50.5 ± 5.2 222.2 ± 18.2 823.4 ± 32.3 Group 3 40.4 ± 2.7 200.6 ± 12.3 738.4 ± 42.8 Control group 71.5 ± 5.0 243.8 ± 12.7 928.7 ± 21.9

Referring to the results in Table 5, serum ALT and AST were significantly decreased in the test group when the test substance was treated at a high concentration (2,000 mg / kg body weight) as compared with the untreated group. Serum ALT and AST of the control group were significantly increased compared to the untreated group. Similar to serum ALT and AST, serum ALP was significantly lower in the test group than in the untreated group when the test substance was treated at high concentration.

From the above-described experimental results, it was confirmed that the hangover-eliminating beverage of the present invention had the same or better effects than the commercially available hangover-free beverage.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation. Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (7)

delete delete delete delete delete Cultivating the bean sprouts by supplying the cultivation water after germinating the soybeans;
A first extraction step of mixing the skin of 100 parts of soybean sprouts with 20 parts of oligosaccharide, and then extracting the soybean sprouts extract by removing the soybean sprouts after lengthening at 20 ° C for 2 days;
Mixing 5 parts by weight of Hovenous Fruit and 5 parts by weight of pine needles with respect to 100 parts by weight of the bean sprouts extract to obtain a mixture;
A second extraction step of adding water to the mixture at a weight ratio of 10 times, followed by hot extraction at 110 ° C for 6 hours, followed by filtration to extract the combined extract;
Adding the purified water to the combined extract to dilute the combined extract to form 70% by weight of the combined extract and 30% by weight of the purified water,
Wherein the cultivation step is carried out by cultivating the cultivated water at a concentration of 15 ppm in a dark room at 25 캜 for 10 minutes at intervals of 4 hours and cultivating the cultivation for 6 days. A method for producing a hangover-resolved beverage. [Claim 7] The method according to claim 6, wherein the growing step comprises the steps of: a) placing the germinated soybeans in a cultivation vessel and cultivating the cultivated water in a dark room at 20 to 30 DEG C for 5 to 15 minutes at intervals of 2 to 6 hours for primary cultivation for 5 to 7 days; And b) cultivating the bean sprouts which have been firstly cultivated with red light for secondary cultivation for 2 to 4 days. The method according to claim 1, wherein the bean sprouts are bean sprouts. Way.

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