KR101470064B1 - Method for preparing cream composition for treating atopic dermatitis comprising japonica extract and cream composition prepared by the same - Google Patents

Method for preparing cream composition for treating atopic dermatitis comprising japonica extract and cream composition prepared by the same Download PDF

Info

Publication number
KR101470064B1
KR101470064B1 KR1020120022111A KR20120022111A KR101470064B1 KR 101470064 B1 KR101470064 B1 KR 101470064B1 KR 1020120022111 A KR1020120022111 A KR 1020120022111A KR 20120022111 A KR20120022111 A KR 20120022111A KR 101470064 B1 KR101470064 B1 KR 101470064B1
Authority
KR
South Korea
Prior art keywords
elm
atopic dermatitis
cream composition
treating atopic
layer
Prior art date
Application number
KR1020120022111A
Other languages
Korean (ko)
Other versions
KR20130101179A (en
Inventor
박강서
마윤정
신성혜
Original Assignee
(재)예수병원유지재단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (재)예수병원유지재단 filed Critical (재)예수병원유지재단
Priority to KR1020120022111A priority Critical patent/KR101470064B1/en
Publication of KR20130101179A publication Critical patent/KR20130101179A/en
Application granted granted Critical
Publication of KR101470064B1 publication Critical patent/KR101470064B1/en

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/44Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/06Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10Preparation or pretreatment of starting material
    • A61K2236/15Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/53Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)

Abstract

느릅나무 추출액을 함유하는 아토피 피부염 치료용 크림 조성물 제조방법 및 이에 의하여 제조된 느릅나무 추출액을 함유하는 아토피 피부염 치료용 크림이 제공된다.
본 발명의 일 실시예에 따른 느릅나무 추출액을 함유하는 아토피 피부염 치료용 크림 조성물 제조방법은, 0.3㎛ 내지 3㎛ 크기로 분쇄된 느릅나무 분말을 글리세린과 함께 물에 혼입, 용해시키는 단계; 상기 용해액을 3000rpm 속도로 30분간 원심분리하는 단계; 상기 원심분리의 상층액을 분리하여 수상층을 얻은 후, 상기 수상층을 가열하는 단계; 바세린, 세틸아코올, 밀랍, 레시틴 및 천연방부재를 혼합하여 유상층을 제조한 후, 상기 제조된 유상층을 가열하는 단계; 및 상기 수상층과 유상층을 혼합하는 단계를 포함하는 것을 특징으로 한다.
There is provided a method of manufacturing a cream composition for treating atopic dermatitis containing an elm extract and a cream for treating atopic dermatitis containing the elm extract prepared thereby.
The method for preparing a cream composition for treating atopic dermatitis containing an elm extract according to an embodiment of the present invention comprises the steps of mixing and dissolving elm powder ground in a size of 0.3 mu m to 3 mu m together with glycerin in water; Centrifuging the solution at a speed of 3000 rpm for 30 minutes; Separating the supernatant of the centrifugal separation to obtain an aqueous phase layer, and heating the aqueous phase layer; Preparing an oily layer by mixing vaseline, cetyl alcohol, beeswax, lecithin and natural preservative, and then heating the oily layer; And mixing the water-repellent layer and the oil phase layer.

Description

느릅나무 추출액을 함유하는 아토피 피부염 치료용 크림 조성물 제조방법 및 이에 의하여 제조된 느릅나무 추출액을 함유하는 아토피 피부염 치료용 크림{Method for preparing cream composition for treating atopic dermatitis comprising japonica extract and cream composition prepared by the same} TECHNICAL FIELD The present invention relates to a method for preparing a cream composition for treating atopic dermatitis containing an elm extract, and a cream for treating atopic dermatitis containing the elm extract prepared thereby. }

본 발명은 느릅나무 추출액을 함유하는 아토피 피부염 치료용 크림 조성물 제조방법 및 이에 의하여 제조된 느릅나무 추출액을 함유하는 아토피 피부염 치료용 크림에 관한 것으로, 보다 상세하게는 이를 원심분리 방식으로 느릅나무 수상층을 분리하여, 사용하는 느릅나무 추출액을 함유하는 아토피 피부염 치료용 크림 조성물 제조방법 및 이에 의하여 제조된 느릅나무 추출액을 함유하는 아토피 피부염 치료용 크림에 관한 것이다.The present invention relates to a method for producing a cream composition for treating atopic dermatitis containing an elm extract, and a cream for treating atopic dermatitis containing the elm extract prepared thereby. More particularly, the present invention relates to a cream for treating atopic dermatitis, A method of preparing a cream composition for treating atopic dermatitis containing the elm extract used, and a cream for treating atopic dermatitis containing the elm extract prepared thereby.

느릅나무(Ulmus davidiana var. japonica)는 쌍떡잎식물 쐐기풀목 느릅나무과의 낙엽활엽 교목이다. 봄에 어린 잎은 식용으로 사용하며 껍질을 유피라고 하고 특히 뿌리껍질을 유근피라고 한다. 느릅나무에는 종류가 여러가지가 있다.The elm (Ulmus davidiana var. Japonica) is a deciduous broad-leaved arboreous tree with a periwinkle plant Nettieum elm. In spring, young leaves are used for edible purposes, and the skin is called epidermis, and especially the root skin is called epimedium. There are many kinds of elm trees.

그 중 참느릅나무와 둥근 참느릅나무, 좀참느릅나무는 열매가 9~10월에 익고, 당느릅나무, 혹느릅나무, 떡느릅나무 등은 4~5월에 익는다. 이들 나무는 열매 익는 시기와 껍질의 생김새만 다를 뿐 잎 모양이나 약으로의 쓰임새는 같다.Among them, the fruit tree is ripened in September - October, the elm tree, the elm tree, and the pear tree are ripened in April - May. These trees differ only in the appearance of the time of ripe berries and the appearance of the shells, but they are the same as leaves and medicines.

느릅나무는 예로부터 종기나 종창에 효과가 있는 나무로 알려져 왔으며 최근에는 항산화 효과, 아질산염 제거능과 치과 카리에스에 대한 항세균 효과 및 항암효과에 대한 보고가 있다. 동의보감에서 느릅나무는 성질이 평하고 맛이 달고 독이 없으며 부드러워 대소변을 잘 통하게 하고 장, 위의 사열을 없애 장염에 효과적이며 부은 것을 가라앉히고 불면증을 낫게 한다고 하였다. 하지만, 이와 같이 우수한 느릅나무 성분이 아토피 피부염 치료 성분으로 활용한 종래기술은 개시되지 않은 상황이다.Elm has long been known to be effective for boils and swellings. Recently, there have been reports of antioxidative, nitrite removal, anti-bacterial and anti-cancer effects on dental caries. In Donguibogam, elm trees are plain, flavorless, poisonous, and soft, so that they can easily pass through the feces, eliminate the intestines and upper quadrats, become effective in enteritis, calm the swollen and relieve insomnia. However, the prior art utilizing such excellent elm ingredient as a therapeutic ingredient for atopic dermatitis has not been disclosed.

따라서, 본 발명이 해결하려는 과제는 느릅나무 성분을 포함하여, 환자 치료에 유효한, 느릅나무 성분을 포함하는 아토피 피부염 치료용 크림 조성물 제조방법 및 이에 의하여 제조된 아토피 피부염 치료용 크림을 제공하는 것이다.Accordingly, an object to be solved by the present invention is to provide a method for producing a cream composition for treating atopic dermatitis, which comprises an elm ingredient effective for treating a patient, including an elm ingredient, and a cream for treating atopic dermatitis produced thereby.

상기 과제를 해결하기 위하여, 본 발명은 아토피 피부염 느릅나무 추출액을 이용한 아토피 피부염 치료용 크림 조성물 제조방법으로, 상기 방법은 0.3㎛ 내지 3㎛ 크기로 분쇄된 느릅나무 분말을 글리세린과 함께 물에 혼입, 용해시키는 단계;In order to solve the above-mentioned problems, the present invention provides a method for preparing a cream composition for treating atopic dermatitis using an atopic dermatitis elm extract, wherein the elm powder pulverized to a size of 0.3 to 3 탆 is mixed with glycerin, Dissolving;

상기 용해액을 3000rpm 속도로 30분간 원심분리하는 단계; 상기 원심분리의 상층액을 분리하여 수상층을 얻은 후, 상기 수상층을 가열하는 단계; 바세린, 세틸아코올, 밀랍, 레시틴 및 천연방부재를 혼합하여 유상층을 제조한 후, 상기 제조된 유상층을 가열하는 단계; 및 상기 수상층과 유상층을 혼합하는 단계를 포함하는 것을 특징으로 하는 아토피 피부염 치료용 크림 조성물 제조방법을 제공한다. Centrifuging the solution at a speed of 3000 rpm for 30 minutes; Separating the supernatant of the centrifugal separation to obtain an aqueous phase layer, and heating the aqueous phase layer; Preparing an oily layer by mixing vaseline, cetyl alcohol, beeswax, lecithin and natural preservative, and then heating the oily layer; And mixing the water-soluble layer and the oil-phase layer. The present invention also provides a method for preparing a cream composition for treating atopic dermatitis.

본 발명의 일 실시예에서 상기 유상층은 바세린 250, 세틸알코올 20, 밀랍 40, 레시틴 10 및 천연방부제 2 중량부로 이루어진다. In one embodiment of the present invention, the oily layer comprises vaseline 250, cetyl alcohol 20, beeswax 40, lecithin 10, and 2 parts by weight of a natural preservative.

본 발명은 또한 상술한 방법에 의하여 제조된 아토피 피부염 치료용 크림 조성물을 제공한다. The present invention also provides a cream composition for treating atopic dermatitis, which is produced by the above-described method.

본 발명에 따르면, 느릅나무를 분말화한 후, 이를 원심분리 방식으로 상층액과 하부 침전물로 분리하고, 그 상층액을 유효성분으로 포함하는 느릅나무 크림을 제조한다. 본 발명에 따른 느릅나무 크림은 아토피 피부질환 치료에 효과적이다. According to the present invention, after elm is powdered, the elm is separated into an upper layer and a lower layer by centrifugal separation, and an elm cream containing the upper layer as an active ingredient is prepared. The elm cream according to the present invention is effective for treating atopic skin diseases.

도 1은 본 발명의 일 실시예에 따른 아토피 피부염 치료용 크림 조성물 제조방법의 단계도이다.
도 2는 정상쥐 귀 사진이고, 도 3은 Hematoxylin-Eosin 염색 사진이다.
도 4는 아토피 발병쥐-대조크림 20일동안 처치한 쥐의 귀사진이고, 도 5는 아토피 발병쥐-대조크림 20일동안 처치한 쥐의 귀의 Hematoxylin -Eosin 염색 사진이다.
도 6은 아토피 발병 쥐에 대하여 본 발명에 따른 느릅크림 20일동안 처치한 쥐의 귀사진이고, 도 7은 본 발명에 따른 느릅크림으로 치료된 쥐의 귀의 Hematoxylin-Eosin 염색 사진이다.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a block diagram of a method for preparing a cream composition for treating atopic dermatitis according to an embodiment of the present invention.
FIG. 2 is a photograph of a normal mouse ear, and FIG. 3 is a hematoxylin-Eosin staining photograph.
FIG. 4 is a photograph of a mouse ear treated for 20 days of atopic disease-treated mice and FIG. 5 is a photograph of Hematoxylin-Eosin staining of a rat ear treated for 20 days of atopic disease-treated mice.
FIG. 6 is a photograph of a mouse ear treated for 20 days with the nematocuria according to the present invention and FIG. 7 is a photograph showing the hematoxylin-Eosin staining of the rat's ear treated with the nematocrit according to the present invention.

본 발명과 본 발명의 동작상의 이점 및 본 발명의 실시에 의하여 달성되는 목적을 충분히 이해하기 위해서는 본 발명의 바람직한 실시예를 예시하는 첨부 도면 및 첨부 도면에 기재된 내용을 참조하여야만 한다. In order to fully understand the present invention, operational advantages of the present invention, and objects achieved by the practice of the present invention, reference should be made to the accompanying drawings and the accompanying drawings which illustrate preferred embodiments of the present invention.

이하, 첨부한 도면을 참조하여 본 발명의 바람직한 실시예를 설명함으로서, 본 발명을 상세히 설명한다. 그러나, 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며, 설명하는 실시예에 한정되는 것이 아니다. 그리고, 본 발명을 명확하게 설명하기 위하여 설명과 관계없는 부분은 생략되며, 도면의 동일한 참조부호는 동일한 부재임을 나타낸다. Hereinafter, the present invention will be described in detail with reference to the preferred embodiments of the present invention with reference to the accompanying drawings. However, the present invention can be implemented in various different forms, and is not limited to the embodiments described. In order to clearly describe the present invention, parts that are not related to the description are omitted, and the same reference numerals in the drawings denote the same members.

아토피 피부염은 주로 유아기 혹은 소아기에 시작되는 만성적이고 재발성의 염증성 피부질환으로 소양증(가려움증)과 피부건조증, 특징적인 습진을 동반하는 질병이다. 아토피 피부염의 발병 원인은 아직 확실하게 알려져 있지 않은 상태이다. 임상 증상도 피부건조증, 습진 등으로 다양하게 나타나기 때문에 발병 원인이 어느 한 가지로만 설명될 수는 없지만, 환경적인 요인과 유전적인 소인, 면역학적 반응 및 피부보호막의 이상 등이 주요 원인으로 여겨지고 있다. 환경적인 요인으로는 산업화로 인한 매연 등 환경 공해, 식품첨가물 사용의 증가, 서구식 주거 형태로 인한 카펫, 침대, 소파의 사용 증가, 실내 온도 상승으로 인한 집먼지 진드기 등의 알레르기를 일으키는 원인 물질(알레르겐)의 증가 등이 있다.Atopic dermatitis is a chronic, recurrent inflammatory skin disease that starts in infancy or childhood, and is a disease accompanied by pruritus (itching), dry skin, and characteristic eczema. The cause of atopic dermatitis has not been known yet. Clinical symptoms vary widely from dry skin to eczema. Therefore, the cause of the disease can not be explained by any single cause, but environmental factors, genetic predisposition, immunological response, and skin barrier abnormalities are considered to be the main causes. Environmental factors include environmental pollution caused by industrialization, increase in the use of food additives, increase in use of carpet, bed, sofa due to western type of residence, cause of allergy such as house dust mite due to rise of room temperature (allergen ).

따라서 발명원인이 아직까지 불분명한 아토피 피부염의 치료는 다양한 방식으로 시도되고 있으나, 아직까지는 확실한 해결방법이 개시되지 못하는 상황이다. 이러한 문제를 해결하고자 본 발명은 이러한 아토피 피부염 치료용 유효성분으로 원심분리된 아토피 추출액을 아토피 피부염 치료용 크림 조성물의 유효성분으로 사용하는 새로운 기술을 제공한다. Therefore, the treatment of atopic dermatitis, which is still unclear as to the cause of the invention, has been attempted in various ways, but a definite solution has not yet been initiated. In order to solve such a problem, the present invention provides a new technique of using the atopic extract, which is centrifuged as an active ingredient for treating atopic dermatitis, as an active ingredient of a cream composition for treating atopic dermatitis.

도 1은 본 발명의 일 실시예에 따른 아토피 피부염 치료용 크림 조성물 제조방법의 단계도이다.BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a block diagram of a method for preparing a cream composition for treating atopic dermatitis according to an embodiment of the present invention.

도 1을 참조하면, 먼저 느릅나무를 0.3㎛ 내지 3㎛ 크기로 분쇄시킨다. 이러한 분쇄과정에서 생나무인 느릅나무는 진액 등을 배출하면서 분말화된다. 본 발명자는 특히 분쇄과정에서 얻어진 액체가 피부 치료에 특히 유용하다는 점을 발견하였고, 이러한 액체를 물에 용해시키는 방식으로 대량화하였다. Referring to FIG. 1, elm is first ground to a size of 0.3 mu m to 3 mu m. In this grinding process, the elm, which is a green tree, is pulverized while discharging the juice. The present inventors have found that the liquid obtained in the pulverization process is particularly useful for skin treatment, and the liquid is bulked in such a manner that the liquid is dissolved in water.

상기 분쇄된 느릅나무 분말을 글리세린에 혼합한 후, 정제수에 녹이고, 원심분리하는데, 본 발명의 일 실시예에서 상기 원심분리는 3000rpm 이상(5000rpm 이하)의 속도로 30분간 진행되며, 이로써 분쇄과정을 통하여 얻어진 느릅나무 분말 용해액은 상층액과 침전물로 분리된다. 따라서, 본 발명에서 상기 원심분리의 속도와 시간은 본 발명에 따른 느릅나무 추출물로부터 약학적 유효성분을 분리하는 데 매우 중요한 요소이다. 이후 최하단 침전물을 제거하고, 상층액을 분리한 후, 상기 상층액을 가열한다. 이로써 본 발명에 따른 아토피 피부염 치료용 수상층이 제조된다.The pulverized elm powder is mixed with glycerin, dissolved in purified water, and centrifuged. In one embodiment of the present invention, the centrifugation is performed at a speed of 3000 rpm or more (5000 rpm or less) for 30 minutes, The elm liquor solution obtained through this process is separated into supernatant and sediment. Therefore, the rate and time of centrifugation in the present invention is a very important factor for separating a pharmaceutically active ingredient from the elm extract according to the present invention. Thereafter, the lowermost precipitate is removed, the supernatant is separated, and then the supernatant is heated. Thus, an aqueous phase layer for treating atopic dermatitis according to the present invention is prepared.

본 발명에 따른 아토피 피부염 치료용 크림은 수상층 외에 유상층을 포함하며, 유상층은 바세린 10g, 올리브 10g, 산초 3 g, 세틸알코올 1g, 몬타노브 2g, 밀랍 0.5g, 레시틴 2g, 천연방부제(예를 들면 프로폴리스) 2g, 비타민 E 1.5g 을 비이커에 넣고 가열하여 녹이는 방식으로 제조된다.
The cream for treating atopic dermatitis according to the present invention comprises an oil phase layer in addition to the water phase layer, and the oil phase layer is composed of 10 g of vaseline, 10 g of olive, 3 g of sancho, 1 g of cetyl alcohol, 2 g of montanov, 0.5 g of beeswax, 2 g of lecithin, 2 g of propolis, for example) and 1.5 g of vitamin E are placed in a beaker and heated to dissolve.

아토피 피부 치료효과 실험Experiment of atopy skin treatment effect

본 발명자는 본 발명에 따른 크림 조성물이 가지는 우수한 아토피 피부염 치료효과를 확인하기 위하여, 쥐를 대상으로 하는 동물실험을 진행하였다. 이를 위하여, 외과용테이프로 귀를 자극 3번 한 후, 아토피 유도물질인 집진드기추출물과 DNCB를 번갈아가며 바르는 과정을 반복하며 아토피 발병을 유도하였다. 이후, 5주 후 아토피가 발병하고 진행되는 것을 확인한 후에 20일동안 치료물질을 발랐는데, 치료물질로는 PBS, 대조크림 및 본 발명에 따른 느릅크림을 발랐다. The present inventor conducted an animal experiment for rats in order to confirm the excellent therapeutic effect of the cream composition according to the present invention on atopic dermatitis. To this end, the ear was stimulated 3 times with a surgical tape, and then repeatedly applied with atorvastatin, an extract of house dust mite, and DNCB, to induce atopy. After 5 weeks, it was confirmed that atopy developed and progressed. After 20 days, the therapeutic material was applied. PBS, a control cream, and a nymph cream according to the present invention were applied as a therapeutic substance.

정상군Normal group

실험기간동안 아토피유발물질은 바르지 않은 쥐에 치료용 크림 대신에 20일동안 PBS를 매일 발랐다. 도 2는 정상쥐 귀 사진이고, 도 3은 Hematoxylin-Eosin 염색 사진이다.
During the study period, atopy inducers were dosed daily with PBS for 20 days instead of the therapeutic cream in the untreated rats. FIG. 2 is a photograph of a normal mouse ear, and FIG. 3 is a hematoxylin-Eosin staining photograph.

대조군Control group

아토피가 유도되었음을 확인한 후에 크림의 기본 구성성분(본 발명에 따른 느릅나무 성분을 제외한 나머지)만으로 제조한 대조크림을 아토피가 발병한 귀에 20일동안 매일 발랐다.After confirming that the atopy had been induced, the control cream, which was made only with the basic constituents of the cream (except for the elm ingredient according to the invention), was applied daily for 20 days to the atopic-induced ear.

도 4는 아토피 발병쥐-대조크림 20일동안 처치한 쥐의 귀사진이고, 도 5는 아토피 발병쥐-대조크림 20일동안 처치한 쥐의 귀의 Hematoxylin -Eosin 염색 사진이다.
FIG. 4 is a photograph of a mouse ear treated for 20 days of atopic disease-treated mice and FIG. 5 is a photograph of Hematoxylin-Eosin staining of a rat ear treated for 20 days of atopic disease-treated mice.

실험군Experimental group

아토피가 유도되었음을 확인한 후에 유효성분인 느릅을 주성분으로 하는 본 발명의 아토피 피부염 치료용 크림을 아토피가 유도된 귀에 20일 동안 매일 발랐다. After confirming that atopy had been induced, the atopic dermatitis cream of the present invention containing the active ingredient elm as the main ingredient was applied to the atopic-induced ear every day for 20 days.

도 6은 아토피 발병 쥐에 대하여 본 발명에 따른 느릅크림 20일동안 처치한 쥐의 귀사진이고, 도 7은 본 발명에 따른 느릅크림으로 치료된 쥐의 귀의 Hematoxylin-Eosin 염색 사진이다.
FIG. 6 is a photograph of a mouse ear treated for 20 days with the nematocuria according to the present invention and FIG. 7 is a photograph showing the hematoxylin-Eosin staining of the rat's ear treated with the nematocrit according to the present invention.

분석analysis

도 2 내지 7을 참조하면, 본 발명에 따른 느릅나무 크림으로 치료된 귀는 아토피 피부염에 대한 치료효과가 매우 우수하다는 것을 알 수 있다.
2 to 7, it can be seen that the ear treated with the elm cream according to the present invention has a very excellent therapeutic effect on atopic dermatitis.

이상에서 기재된 "포함하다", "구성하다" 또는 "가지다" 등의 용어는, 특별히 반대되는 기재가 없는 한, 해당 구성 요소가 내재될 수 있음을 의미하는 것이므로, 다른 구성 요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것으로 해석되어야 한다. 기술적이거나 과학적인 용어를 포함한 모든 용어들은, 다르게 정의되지 않는 한, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미를 가진다. 사전에 정의된 용어와 같이 일반적으로 사용되는 용어들은 관련 기술의 문맥 상의 의미와 일치하는 것으로 해석되어야 하며, 본 발명에서 명백하게 정의하지 않는 한, 이상적이거나 과도하게 형식적인 의미로 해석되지 않는다.
It is to be understood that the terms "comprises", "comprising", or "having" as used in the foregoing description mean that the constituent element can be implanted unless specifically stated to the contrary, But should be construed as further including other elements. All terms, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, unless otherwise defined. Commonly used terms, such as predefined terms, should be interpreted to be consistent with the contextual meanings of the related art, and are not to be construed as ideal or overly formal, unless expressly defined to the contrary.

Claims (3)

느릅나무 추출액을 이용한 아토피 피부염 치료용 크림 조성물 제조방법으로, 상기 방법은
생나무 상태인 느릅나무를 0.3㎛ 내지 3㎛ 크기로 분쇄시키는 단계;
상기 분쇄시키는 단계에서 생성된 느릅나무 분말과 진액을 글리세린과 함께 물에 혼입, 용해시키는 단계;
상기 용해액을 3000rpm 속도로 30분간 원심분리하는 단계;
상기 원심분리된 상층액을 분리하여 수상층을 얻은 후, 상기 수상층을 가열하는 단계;
바세린, 세틸아코올, 밀랍, 레시틴 및 천연방부제를 혼합하여 유상층을 제조한 후, 상기 제조된 유상층을 가열하는 단계; 및
상기 수상층과 유상층을 혼합하는 단계를 포함하는 것을 특징으로 하며, 상기 유상층은 바세린 250, 세틸알코올 20, 밀랍 40, 레시틴 10 및 천연방부제 2 중량부로 이루어진 것을 특징으로 하는 아토피 피부염 치료용 크림 조성물 제조방법.
A method for producing a cream composition for treating atopic dermatitis using an elm extract,
Pulverizing the elm tree in a green state to a size of 0.3 mu m to 3 mu m;
Mixing the elm powder and the juice produced in the pulverizing step together with glycerin in water to dissolve the elm powder;
Centrifuging the solution at a speed of 3000 rpm for 30 minutes;
Separating the centrifuged supernatant to obtain an aqueous phase layer, and heating the aqueous phase layer;
Preparing an oily layer by mixing vaseline, cetyl alcohol, beeswax, lecithin and a natural preservative, and then heating the oily layer; And
Characterized in that the oil phase layer is composed of vaseline 250, cetyl alcohol 20, beeswax 40, lecithin 10 and 2 parts by weight of a natural preservative. ≪ / RTI >
삭제delete 제 1항에 따른 방법에 의하여 제조되며, 느릅나무 추출액을 유효성분으로 함유하는 아토피 피부염 치료용 크림 조성물.A cream composition for the treatment of atopic dermatitis, which is prepared by the method according to claim 1 and contains an elm extract as an active ingredient.
KR1020120022111A 2012-03-05 2012-03-05 Method for preparing cream composition for treating atopic dermatitis comprising japonica extract and cream composition prepared by the same KR101470064B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020120022111A KR101470064B1 (en) 2012-03-05 2012-03-05 Method for preparing cream composition for treating atopic dermatitis comprising japonica extract and cream composition prepared by the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020120022111A KR101470064B1 (en) 2012-03-05 2012-03-05 Method for preparing cream composition for treating atopic dermatitis comprising japonica extract and cream composition prepared by the same

Publications (2)

Publication Number Publication Date
KR20130101179A KR20130101179A (en) 2013-09-13
KR101470064B1 true KR101470064B1 (en) 2014-12-12

Family

ID=49451429

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020120022111A KR101470064B1 (en) 2012-03-05 2012-03-05 Method for preparing cream composition for treating atopic dermatitis comprising japonica extract and cream composition prepared by the same

Country Status (1)

Country Link
KR (1) KR101470064B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200050030A (en) 2018-10-31 2020-05-11 (주)동구바이오제약 Composition for external application having improved stability containing elm extracts

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102098966B1 (en) * 2017-11-10 2020-04-08 남부대학교산학협력단 Composition having antibacterial activity for skin pathogens and trichophyton
KR102347175B1 (en) * 2019-11-04 2022-01-03 이하림 Atopy cream manufacturing method and apparatus containing pumpkin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040050154A (en) * 2002-12-09 2004-06-16 성인영 Composition for wound healing containing extract of Japanese Elm root bark

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040050154A (en) * 2002-12-09 2004-06-16 성인영 Composition for wound healing containing extract of Japanese Elm root bark

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
한국식품과학회지. 제42권, 제1호, pp.109-114 *
한국식품과학회지. 제42권, 제1호, pp.109-114*

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200050030A (en) 2018-10-31 2020-05-11 (주)동구바이오제약 Composition for external application having improved stability containing elm extracts

Also Published As

Publication number Publication date
KR20130101179A (en) 2013-09-13

Similar Documents

Publication Publication Date Title
JP5860577B2 (en) Claudin production promoter and occludin production promoter
KR101360231B1 (en) Pharmaceutical composition for preventing or treating liver cancer comprising herbal extracts
CN104857073B (en) Asian puccoon Skin-care Chinese medicine composition and Asian puccoon cold cream and preparation method thereof
US20090117146A1 (en) System and method for promoting hair growth and improving hair and scalp health
CN109303794B (en) Selenium-rich cardamine violifolia extract and application thereof in liver protection
KR101379029B1 (en) Method for preparing japonica extract and cream and band comprising the same
KR101165716B1 (en) Compositions for prevention and treatment of allergic disease comprising the fractions from the extracts of Gardenia jasminoides as an active ingredient
KR101470064B1 (en) Method for preparing cream composition for treating atopic dermatitis comprising japonica extract and cream composition prepared by the same
CN103652859A (en) Health-care food with oxidation resistance and preparation method thereof
KR101934794B1 (en) Composition for preventing, improving or treating atopic dermatitis comprising extract mixture of Diospyros lotus leaf and grape fruit stem as effective component
US20180092958A1 (en) Composition for preventing, alleviating or treating neurodegenerative diseases
Malík et al. Nootropic herbs, shrubs, and trees as potential cognitive enhancers
KR101486484B1 (en) A food composition for the improvement or prevention of asthma comprising extract of allium hookeri
KR101914239B1 (en) The useful for hair growth in external application composition of the manufacturing methods
KR101794006B1 (en) Anti inflammatory comprising plant extract
JP5302632B2 (en) Oral composition for prevention and treatment of atopic dermatitis containing plant extract
KR20130040579A (en) Pharmaceutical composition for preventing or treating renal cell carcinoma comprising herbal extracts
KR102253702B1 (en) Cosmetic composition comprising seaweed ingredients and seawater
KR20090075950A (en) Therapeutical compositions for obstinate atopic dermatitis
KR101835661B1 (en) A cosmetic composition containing ceriporia lacerata extract for alleviating skinitching and preventing dermatitis and cosmetics container including the same
JP2018522923A (en) Slimming and skin care composition and method for preparing the same
JP5597642B2 (en) Antioxidant cosmetic composition containing smoked and processed sweet chrysanthemum and cinnamon extracts
CN102058731B (en) Preparation of chaste tree twig leaf and fruit extract and anti-inflammatory medical application thereof
KR101221623B1 (en) Food Composition for Stress Relaxation, Fatigue Recovery or Athletic Performance Enhancement Comprising Extract of root of vitis
KR100900197B1 (en) Compositions of ointments controlling skin immune system containing specific herbal extract complex

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E90F Notification of reason for final refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20181025

Year of fee payment: 5

R401 Registration of restoration