CN102058731B - Preparation of chaste tree twig leaf and fruit extract and anti-inflammatory medical application thereof - Google Patents
Preparation of chaste tree twig leaf and fruit extract and anti-inflammatory medical application thereof Download PDFInfo
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- CN102058731B CN102058731B CN201010617649A CN201010617649A CN102058731B CN 102058731 B CN102058731 B CN 102058731B CN 201010617649 A CN201010617649 A CN 201010617649A CN 201010617649 A CN201010617649 A CN 201010617649A CN 102058731 B CN102058731 B CN 102058731B
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Abstract
The invention discloses a chaste tree twig fruit extract as well as a preparation method and medical application thereof. The preparation method comprises the following steps of: weighing dry chaste tree twig leaves or fruits thereof, crushing the chaste tree twig leaves or fruits, adding water or lower alcohol and other high polarity solvents or a mixed solvent of high polarity solvents and water, heating, refluxing and extracting the solution, filtering the extract through a gauze, extracting the filter cake twice under the same conditions, merging the filter liquids, recovering the supernatant liquid after centrifugation, and freezing and drying the supernatant liquid to obtain the extract. The invention is characterized by using the extract of chaste tree twig leaves and fruits as a raw material to prepare safe and non-toxic medicinal products, health foods, food additives and compositions thereof which can improve the status of organisms and can be administrated for long term. In addition, the extract of chaste tree twig leaves and fruits can also be used for preparing various dosage forms of external skin agents or cosmetics.
Description
Technical field
The present invention discloses the preparation and the antiphlogistic medical application thereof of a kind of Vitex chinensis Mill. leaf and fruit extract, relates to natural plant extracts, belongs to traditional Chinese medical science pharmaceutical technology field.
Background technology
The plant Vitex chinensis Mill. that the present invention relates to (Vitex negundo var. heterophylla) belong to Verenaceae (Verbenaceae), the Vitex plant (Vitex L.) of Herba Viticis Cannabifoliae subfamily (Viticoideae Briq.).The Vitex plant is perennial arbor or shrub, and record Vitex plant comprises 14 kinds, 7 mutation and 3 kinds of hypotypes in Chinese Plants will.Principal item in this platymiscium grows on the south the Changjiang river mostly, and the part kind grows in the Qinling Mountains and Qinghai-Tibet the Northwest.Wherein, few species phytoclimate adaptabilities such as Vitex chinensis Mill. are strong, are dispersed throughout North China, the broad regions in northwest and northeast.People have found to comprise flavone, terpenoid, lignanoid, sterol, anthracene class and the dissimilar hundreds of chemical compounds of volatilization wet goods from the Vitex plant up to now, and flavone that wherein is rich in and lignanoid are considered to topmost active component in this platymiscium.Some experimental results in recent years confirm that the flavone compound that is rich in the Vitex plant can be used for slow down aging, prevent and treat effects such as cardiovascular disease, show development and application values preferably.But up to now antiinflammatory, the antiallergic of Vitex chinensis Mill. leaf and fruit water solubility extract and the pharmacology activity research that improves aspects such as liver function are not seen any report as yet, simultaneously to Vitex chinensis Mill. leaf and the effective protection of fruit on application facet such as prevent disease, healthy function, medicine, health food and cosmetics also are ignorant property right.
Summary of the invention
The present invention provides a kind of Vitex chinensis Mill. Vitex chinensis Mill. leaf and fruit extract, has antiinflammatory and antioxidation.
The invention also discloses the method for preparing of said extracted thing, be applicable to suitability for industrialized production.
The present invention further provides this extract to be used to prepare prevention and the medicinal purposes of treatment inflammation.
The present invention further provides this extract to be used to the purposes for preparing the prevention and improve inflammation food.
The present invention further provides this extract to be used to prepare the purposes of improving scytitis and anaphylactoid medicine or cosmetics.
Vitex chinensis Mill. Vitex chinensis Mill. leaf disclosed by the invention and fruit extract,Be to obtain through following method:
Take by weighing dry Vitex chinensis Mill. leaf or its fruit and pulverize the back and add intensive polar solvents such as water or lower alcohol, the perhaps mixed solvent of itself and water, heating and refluxing extraction; Extract is after gauze filters, and filter cake reuse the same terms extracts twice, closes filtrating; And, promptly get through the lyophilization of centrifugal recovery supernatant.
The extract that is extracted possibly or reclaim macromole deposits such as polysaccharide, diterpene, flavone, lignanoid, volatile oil and protein for micromolecular compound.
But, select water, ethanol or aquiferous ethanol to be advisable so consider to extract solvent from safety perspective seeing that Vitex chinensis Mill. leaf and fruit extract are to develop as final products with medicament or health food as far as possible.
The ratio of Vitex chinensis Mill. leaf and fruit and solvent does not have specific limited during extraction, but the ratio of general Vitex chinensis Mill. leaf and fruit and solvent is with 1: (1～50) scope is advisable.
The present invention does not have specific limited to extracting temperature, all can use to the solvent boiling point scope from room temperature.
If adopt water extraction then temperature should suitably improve more satisfactory.
Method for distilling can adopt heating extraction, supersound extraction, microwave extraction etc. multiple, but also several different methods combines application.
Extraction time, scope could from 10 seconds to 24 hours.
Preferred extraction process is following:
Take by weighing dry Vitex chinensis Mill. leaf or its fruit and pulverize intensive polar solvents such as 8 ~ 10 times of water gagings of back interpolation or lower alcohol; The perhaps mixed solvent of itself and water, 80 ~ 90 ℃ of hot refluxs were extracted 80 ~ 120 minutes, and extract is after gauze filters; Filter cake reuse the same terms extracts twice; Close filtrating, and reclaim the supernatant lyophilization after centrifugal 15 minutes, promptly get through 1500rpm.
Be used to prevent and improve various other clinical symptoms because of inflammation or inflammation cause.And can take for a long time, safely, the pharmaceuticals, food, health food, cosmetics and the constituent thereof that have no side effect.
It is natural plants that Vitex chinensis Mill. leaf and fruit extract have raw material sources, low production cost, and technological process is simple, characteristics such as product yield height.The production technology of Vitex chinensis Mill. leaf of the present invention and fruit extract is that employing Vitex chinensis Mill. leaf and fruit are raw material, and process oven dry, pulverizing, extraction get final product enrichment and recovery obtains extract.
Below experiment shows that extract of the present invention is in prevention with improve the purposes because of inflammation:
Test 1. Vitex chinensis Mill. leaf and fruit extract and propionibacterium acnes and lipopolysaccharide are brought out the improvement effect of mouse immune hepatitis
Experiment use male 7 age in week Kunming mouse, available from Guangdong Medical Lab Animal Center.The raising condition is 23 ± 2 ℃, and lighting hours 12 hours/day (7:00～19:00), 5/rearging cage (235 * 325 * 170 mm).Solid feed is bought by Guangdong Medical Lab Animal Center, freely drinks water, and experimentizes after raising a week.10/group of mice; Via the hot deactivation propionibacterium acness of tail vein injection 20 mg/kg (Propiopionibacteriumacnes) normal saline solution; Propionibacterium acnes load tail vein injection 5 μ gkg-1 lipopolysaccharide (Lipopo lysaccharide)-normal saline solutions after 5 days make mice produce hepatitis.After 5 hours, all mices are taked blood through heart under the etherization condition, and centrifugal treating is measured blood plasma alanine aminotransferase (ALT) activity level with reitman-frankel method.Vitex chinensis Mill. leaf and fruit extract are in the hot deactivation propionibacterium acnes oral administration preceding 1 day continuous 7 days of every day 1 time of loading, and before 30 minutes, matched group is the co-content aqueous solution in the lipopolysaccharide injection in the last administration.Positive drug group cyclosporin A is from propionibacterium acnes-lipopolysaccharide lumbar injection back 1 day next day of loading.Experimental result is represented with mean ± SD, does the significant difference calibrating with Student t-test.Experimental result is as shown in table 1, and the blood plasma alanine aminotransferase level that Vitex chinensis Mill. leaf and fruit extract can effectively improve because of hot deactivation propionibacterium acnes-lipopolysaccharide load mice causes raises.And demonstrate certain dosage interdependence and suppress effect.
Table 1 Vitex chinensis Mill. leaf and fruit extract are to the influence of propionibacterium acnes-lipopolysaccharide load mice plasma alanine aminotransferase
| Group | Dosage (mg/kg) | Number of animals | Alanine aminotransferase (IU/L) |
| Normal control | _ | 10 | 15.62±2.08 |
| The model contrast | ? | ? | 135.38±15.69 ## |
| Cyclosporin A | 1 | 10 | 23.08±5.83**? |
| Embodiment 1 | 500 | 10 | 36.02±2.61** |
| Embodiment 4 | 125 | 10 | ?41.05±3.13** |
| Embodiment 2 | 500 | 10 | 39.32±3.44** |
| Embodiment 3 | 125 | 10 | 77.62±10.04* |
| Embodiment 5 | 125 | 10 | 42.05±3.13** |
##: expression is compared P with the normal control group<0.01,
*: expression is compared P with model group, and < 0.01, *: expression and model group be P < 0.05 relatively
Test 2. Vitex chinensis Mill. leaf and fruit extract and Oleum Tiglii is brought out the improvement effect of mouse ear edge inflammation
Experiment use male 7 age in week Kunming mouse, available from Guangdong Medical Lab Animal Center.The raising condition is 23 ± 2 ℃, and lighting hours 12 hours/day (7:00～19:00), 5/rearging cage (235 * 325 * 170 mm).Solid feed is bought by Guangdong Medical Lab Animal Center, freely drinks water, and experimentizes after raising a week.Test every group of 10 mices; At first 0.3% Oleum Tiglii (Sigma) is spread upon mouse right ear edge central part according to 25 μ l/ ear dosage, press 0.1ml/10g body weight dosage after 3 hours and in mouse tail vein, inject 1% Ai Wensilan (Evans blue) normal saline solution.Injection Ai Wensilan normal saline one as a child after, take off two ears with diameter 8mm card punch and weigh, calculate inflammation edema rate and administration inhibition effect.Simultaneously; With reference to katayama method (A new method for extraction of extravasated dye in the skin and the influence of fasting stress on passive cutaneous anaphylaxis in guinea pigs and rats. Microbiol. Immunol. 1978:22 (2): 89-101); The ear edge is put into the 1N KOH aqueous solution of 1ml; 37 ℃ are soaked after 24 hours down; Add 9ml 0.6N phosphoric acid-acetone (5:13) solution, fully stir back centrifugalize (3000rpm/15 minute).Take out supernatant and measure absorbance, calculate the Ai Wensilan amount as the vascular permeability index, analytical judgment edema situation in the 620nm wavelength.Vitex chinensis Mill. leaf and fruit extract be twice oral administration before the experiment day before yesterday and Oleum Tiglii are smeared 1 hour.Matched group is given with the capacity aqueous solution, and the medicine matched group is with anti-inflammatory drug dexamethasone (Dexamethasone) oral administration of 1mg/kg before Oleum Tiglii is smeared 1 hour.Experimental result is represented with mean ± SD, does the significant difference calibrating with Student t-test.Experimental result is as shown in table 2, brings out mouse ear edge inflammation with aqueous solution matched group Oleum Tiglii and compares, and Vitex chinensis Mill. leaf and fruit extract oral administration also demonstrate certain dosage interdependence and suppress effect.
Table 2 Vitex chinensis Mill. leaf and fruit extract bring out the inhibitory action of mouse ear edge inflammation to Oleum Tiglii
| Group | Dosage (mg/kg) | Number of animals | Ear edge edema (mg) | Ai Wensilan seepage discharge (μ g/ ear) |
| The model contrast | _ | 10 | 15.5±4.7 | 5.38±1.14 |
| Dexamethasone | 1 | 10 | 1.35±0.7** | ? 0.54±0.80 ** |
| Embodiment 1 | 500 | 10 | 9.6±2.7** | 2.92±0.92* |
| Embodiment 4 | 125 | 10 | 11.1±1.9** | ?3.83±1.24* |
| Embodiment 2 | 500 | 10 | 10.6±2.2** | 3.44±0.93* |
| Embodiment 3 | 125 | 10 | 14.2±2.9 | 4.65±1.22 |
| Embodiment 5 | 125 | 10 | 10.1±1.9** | 3.93±1.24* |
*: expression is compared P with model group, and < 0.01, *: expression and model group be P < 0.05 relatively
Test 3. Vitex chinensis Mill. leaf and fruit extract on Carrageenan and bring out the improvement effect of mice foot swelling
Experiment use male 7 age in week Kunming mouse, available from Guangdong Medical Lab Animal Center.The raising condition is 23 ± 2 ℃, and lighting hours 12 hours/day (7:00～19:00), 5/rearging cage (235 * 325 * 170 mm).Solid feed is bought by Guangdong Medical Lab Animal Center, freely drinks water, and experimentizes after raising a week.Test every group of 10 mices, during experiment that mice left side lower limb are stretching, using No. 4 self-sustaining sole of the foot of needle applicator middle and lower parts implantation concentration that makes progress is the carrageenin aqueous solution 10 μ l of 20 mg/ml, sets up carrageenin and causes mice pedal swelling model.After causing scorching 4 hours, it is the highest that inflammatory symptom reaches basically.Measure normal and swelling foot sole of the foot thickness with Digimatic slide calliper rule (precision 0.01 mm), analytical judgment edema situation.Vitex chinensis Mill. leaf and fruit extract are in causing scorching preceding 1 day and causing scorching twice administration in preceding 30 minutes.Matched group is given with the capacity aqueous solution, the medicine matched group with the anti-inflammatory drug dexamethasone in causing scorching preceding 1 day and causing scorching preceding 30 minutes twice oral administration.Experimental result is represented with mean ± SD, does the significant difference calibrating with Student t-test.Experimental result is as shown in table 3, and Vitex chinensis Mill. leaf and fruit extract oral administration can effectively improve smears the caused pedal swelling of carrageenin.
Table 3 Vitex chinensis Mill. leaf and fruit extract bring out the inhibitory action of mice foot swelling on Carrageenan
| Group | Dosage (mg/kg) | Number of animals | Pedal swelling thickness (mm) |
| The model contrast | ? | 10 | 1.63±0.19 |
| Dexamethasone | 1 | 10 | ?0.42±0.21**? |
| Embodiment 1 | 500 | 10 | ?1.22±0.13** |
| Embodiment 4 | 125 | 10 | 1.39±0.22* |
| Embodiment 2 | 500 | 10 | 1.42±0.11* |
| Embodiment 3 | 125 | 10 | 1.55±0.32 |
| Embodiment 5 | 125 | 10 | 1.29±0.22* |
*: expression is compared P with model group, and < 0.01, *: expression and model group be P < 0.05 relatively
Test 4. Vitex chinensis Mill. leaf and fruit extract and DNFB is brought out the improvement effect of mice contact dermatitis
Experiment use male 7 age in week Kunming mouse, available from Guangdong Medical Lab Animal Center.The raising condition is 23 ± 2 ℃, and lighting hours 12 hours/day (7:00～19:00), 5/rearging cage (235 * 325 * 170 mm).Solid feed is bought by Guangdong Medical Lab Animal Center, freely drinks water, and experimentizes after raising a week.Test every group of 10 mices, test the 1st day at the DNFB-95% of mouse back subcutaneous injection 1.5% alcoholic solution 50 μ l as the antigen enhanced sensitivity.Enhanced sensitivity is accomplished after 5 days, on mouse right ear, is coated with 1%DNFB acetone 2 olive oil (4: 1) solution 25 μ l as antigen challenge on the 6th day, makes mice produce contact dermatitis.After the sensitization 3 hours, press 0.1ml/10g body weight dosage and in mouse tail vein, inject 1% Ai Wensilan (Evans blue) normal saline solution.Injection Ai Wensilan normal saline one as a child after, take off two ears with diameter 8mm card punch and weigh, calculate inflammation edema rate and administration inhibition effect.Simultaneously, the ear edge is put into the 1N KOH aqueous solution of 1ml, 37 ℃ were soaked down after 24 hours, added 9ml 0.6N phosphoric acid-acetone (5:13) solution, fully stirred back centrifugalize (3000rpm/15 minute).Take out supernatant and measure absorbance, calculate the Ai Wensilan amount as the vascular permeability index, analytical judgment edema situation in the 620nm wavelength.Vitex chinensis Mill. leaf and fruit extract in the experiment day before yesterday and DNFB smear attack 1 hour before oral administration once a day.Matched group is given with the capacity aqueous solution, the medicine matched group with the anti-inflammatory drug dexamethasone in DNFB smear attack 1 hour before oral administration of 1mg/kg.Experimental result is represented with mean ± SD, does the significant difference calibrating with Student t-test.Experimental result is as shown in table 4, brings out mouse ear edge contact dermatitis with aqueous solution matched group DNFB and compares, and Vitex chinensis Mill. leaf and fruit extract oral administration demonstrate certain dosage interdependence and suppress effect.
Table 4 Vitex chinensis Mill. leaf and fruit extract bring out the inhibitory action of mice contact dermatitis to DNFB
| Group | Dosage (mg/kg) | Number of animals | Ear edge edema (mg) | Ai Wensilan seepage discharge (μ g/ ear) |
| The model contrast | _ | 10 | 21.1±3.8 | 4.13±0.72 |
| Dexamethasone | 1 | 10 | ?0.81±0.6** | ? 0.44±0.67 ** |
| Embodiment 1 | 500 | 10 | ?12.4±2.5** | 2.79±0.82* |
| Embodiment 4 | 125 | 10 | ?16.1±2.1* | ?3.78±0.90* |
| Embodiment 2 | 500 | 10 | ?15.2±1.8* | ?3.91±1.11* |
| Embodiment 3 | 125 | 10 | 19.4±3.2 | 4.12±1.30 |
| Embodiment 5 | 125 | 10 | 15.1±2.1* | 3.98±0.90* |
*: expression is compared P with model group, and < 0.01, *: expression and model group be P < 0.05 relatively
Research contents of the present invention prompting Vitex chinensis Mill. leaf and fruit extract are to autoallergic and have its inflammatory reaction to have to suppress or alleviate effect.Of the present invention is that providing with Vitex chinensis Mill. leaf and fruit extract is raw material, improves fuselage state and can take for a long time, safely, the pharmaceuticals, health food, food additive and the constituent thereof that have no side effect.In addition, Vitex chinensis Mill. leaf and fruit extract are also as the various dosage forms of skin preparations for extenal use or cosmetic use.
Can use water extraction after Vitex chinensis Mill. leaf and fruit are smashed, also can with acute solvent such as the lower alcohols of variable concentrations, acetone or arbitrarily intermixture extract.When extracting, along with the increase of alcohol, the response rate of active component can increase to some extent in Vitex chinensis Mill. leaf and the fruit.Although the water extraction effect extracts not as adding with alcohol, along with water temperature increases, and the prolongation of extraction time, also can reach with alcohol extraction and follow the example of suitable effect.Therefore, as far as the extraction of Vitex chinensis Mill. leaf and fruit, above-mentioned several method and its combination in any all can obtain extract of the present invention.Sign in Vitex chinensis Mill. leaf and fruit extract and develop as final products, select ethanol to be advisable as far as possible so consider to extract solvent from safety perspective with medicament, food and cosmetics.The ratio that extracts Vitex chinensis Mill. and solvent does not have specific limited, but general Vitex chinensis Mill. is advisable with 1:1～50 scopes with the ratio of solvent.The present invention does not have specific limited to extracting temperature, and is all applicable to the solvent boiling point scope from room temperature.Certainly room temperature, normal pressure and in the solvent boiling point scope, extract ideal.Extraction time, scope could from 10 seconds to 24 hours.
Vitex chinensis Mill. leaf provided by the invention and fruit extract can be put on market by multiple peroral dosage form, are used for prevention, improve and the treatment inflammation.Certainly, the Vitex chinensis Mill. seed extract also can combine with pharmaceutical carriers such as excipient commonly used and preparationization.Also can add preparation additives such as some antiseptic, antioxidant, coloring agent, sweeting agent in case of necessity.The relatively more suitable sub-excipient of Vitex chinensis Mill., general consideration has lactose, white sugar, D-mannitol, starch, crystalline cellulose etc.Lubricant generally should adopt magnesium stearate, calcium stearate, Pulvis Talci etc.Solvent uses exquisite water, ethanol, propylene glycol etc. usually.Antiseptic generally adopts methaform, dihydroxy acetic acid etc.Antioxidant is more suitable with sulphite, ascorbic acid etc. usually.
Vitex chinensis Mill. leaf provided by the invention and fruit extract, can its prototype, extract or powder add to be used in the goods oral.Vitex chinensis Mill. leaf and fruit extract also can combine to process certain beverage product with the Other Drinks raw material, like soda pop, fruit drink, sports drink etc.Vitex chinensis Mill. leaf and fruit extract food it is also conceivable that daily breads such as processing cookies, chewing gum class, snack class.
Vitex chinensis Mill. leaf among the present invention and fruit extract also can prototypes, aqueous solution dilution back uses as skin preparations for extenal use.Can certainly like dosage forms such as aerosol, liquid preparation, suspensoid, Emulsion, ointment, semifluid preparation, liniment, lotions cosmetics, pharmaceuticals consumption of market be provided through combine to process certain preparation with pharmaceutical carrier.
Good effect of the present invention is: it is in propionibacterium acnes and lipopolysaccharide bring out the animal experimental models such as improvement effect of mouse immune hepatitis, to be able to confirm that the prevention as inflammation of Vitex chinensis Mill. leaf and fruit extract, improvement and therapeutic agent are provided, and confirms that based on The above results the present invention sets up.The Vitex chinensis Mill. seed extract can be regulated according to application person's conditions such as health status, age, sex and body weight dosage among the present invention.
The specific embodiment
Through following examples the present invention is described for example further; And do not limit the present invention in any way; Under the prerequisite that does not deviate from technical solution of the present invention, any change or change that those of ordinary skills that the present invention did are realized easily all will fall within the claim scope of the present invention.
Embodiment 1
Accurately take by weighing dry Vitex chinensis Mill. leaf 500g, use the broken back of pulverizer micropowder to reclaim the Vitex chinensis Mill. leaf of fine-powdered, add 10 times of water gagings; 80 ℃ of hot refluxs were extracted 120 minutes, and extract is after gauze filters, and filter cake reuse the same terms extracts twice; Close filtrating, and reclaim the supernatant lyophilization after centrifugal 15 minutes, obtain the Vitex chinensis Mill. leaf extract through 1500rpm; Extraction ratio is 18.2%
Embodiment 2
Accurately take by weighing dry Vitex chinensis Mill. fruit 500g, use the broken back of pulverizer micropowder to reclaim the Vitex chinensis Mill. leaf of fine-powdered, add 8 times of water gagings; 90 ℃ of hot refluxs were extracted 120 minutes, and extract is after gauze filters, and filter cake reuse the same terms extracts twice; Close filtrating, and reclaim the supernatant lyophilization after centrifugal 15 minutes, obtain the Vitex chinensis Mill. leaf extract through 1500rpm; Extraction ratio is 9.2%
Embodiment 3
With adding 9 times of amount 60% soak with ethanol 24 h after the dry Vitex chinensis Mill. fruit pulverizing; Reflux, extract, 120 minutes; Extract 3 times, being concentrated into relative density is 1.02 (45 ℃), is that 1500rpm reclaims the supernatant concentrating under reduced pressure after centrifugal 15 minutes with the rotating speed; Can obtain the Vitex chinensis Mill. fruit extract, going out extraction ratio is 5.91%.
Embodiment 4
With adding 9 times of amount 70% soak with ethanol 24 h after the dry Vitex chinensis Mill. leaf pulverizing, reflux, extract, 120 minutes is extracted 3 times; Being concentrated into relative density is 1.02 (45 ℃); With the rotating speed is that 1500rpm reclaims the supernatant concentrating under reduced pressure after centrifugal 15 minutes, can obtain the Vitex chinensis Mill. fruit extract, and going out extraction ratio is 19.1%.
Embodiment 5
With adding 9 times of amount 80% soak with ethanol 24 h after the dry Vitex chinensis Mill. leaf pulverizing, reflux, extract, 120 minutes is extracted 3 times; Being concentrated into relative density is 1.02 (45 ℃); With the rotating speed is that 1500rpm reclaims the supernatant concentrating under reduced pressure after centrifugal 15 minutes, can obtain the Vitex chinensis Mill. fruit extract, and going out extraction ratio is 19.8%.
Claims (1)
1. Vitex chinensis Mill. Vitex chinensis Mill. leaf and fruit extract are used to prepare the purposes of prevention and treatment inflammation medicine; Described extract is to obtain through following method: take by weighing dry Vitex chinensis Mill. leaf or its fruit and pulverize 8 ~ 10 times of water gagings of back interpolation or lower alcohol intensive polar solvent; The perhaps mixed solvent of itself and water, 80 ~ 90 ℃ of hot refluxs were extracted 80 ~ 120 minutes, and extract is after gauze filters; Filter cake reuse the same terms extracts twice; Close filtrating, and reclaim the supernatant lyophilization after centrifugal 15 minutes, promptly get through 1500rpm.
2, Vitex chinensis Mill. Vitex chinensis Mill. leaf and fruit extract are used to prepare the purposes of preventing and improving inflammation food; Described extract is to obtain through following method: take by weighing dry Vitex chinensis Mill. leaf or its fruit and pulverize 8 ~ 10 times of water gagings of back interpolation or lower alcohol intensive polar solvent; The perhaps mixed solvent of itself and water, 80 ~ 90 ℃ of hot refluxs were extracted 80 ~ 120 minutes, and extract is after gauze filters; Filter cake reuse the same terms extracts twice; Close filtrating, and reclaim the supernatant lyophilization after centrifugal 15 minutes, promptly get through 1500rpm.
3, Vitex chinensis Mill. Vitex chinensis Mill. leaf and fruit extract are used to prepare the purposes of improving scytitis and anaphylactoid medicine or cosmetics; Described extract is to obtain through following method: take by weighing dry Vitex chinensis Mill. leaf or its fruit and pulverize 8 ~ 10 times of water gagings of back interpolation or lower alcohol intensive polar solvent; The perhaps mixed solvent of itself and water, 80 ~ 90 ℃ of hot refluxs were extracted 80 ~ 120 minutes, and extract is after gauze filters; Filter cake reuse the same terms extracts twice; Close filtrating, and reclaim the supernatant lyophilization after centrifugal 15 minutes, promptly get through 1500rpm.
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