KR20190035139A - A ccosmetic or food composition comprising ligularia senocephala extract and method of a hangover beverage using thereof - Google Patents

Abstract

The present invention relates to a cosmetic or food composition containing a Ligularia stenocephala extract and a method for producing an anti-hangover beverage using the same. The present invention provides a composition for cosmetics and a composition for health food for improving liver function, comprising a Ligularia stenocephala extract as an active ingredient. The health food prepared by using the Ligularia stenocephala extract can be used for improving and treating liver diseases by providing an antioxidant effect and suppressing hepatotoxicity to improve liver function, and cosmetics having an antioxidant effect can also be effectively used.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic or food composition containing an extract of Mandarin, as an active ingredient, and a method for producing a hangover-free beverage using the same.

More particularly, the present invention relates to a composition containing an extract of Mandarb and a method of producing a hangover drink using the same, and more specifically, to a method for producing a Mandarin extract comprising Ligularia stenocephala (Maxim.) Matsum. & Koidz. ) Extract and a hangover-free beverage using the same.

The liver is the most active organ in the body of the human body, which is located between the body's digestive system and the systemic circulatory system. It protects the body from exogenous substances coming in from the outside and contains a large number of nutrients, enzymes, It is also used as a chemical factory in the human body because it functions to produce and process components.

Since the in vivo substance entering the living body passes through the liver, the liver has a higher risk of being exposed to many toxic substances than the other organs in addition to the nutrients. In addition, the liver is regenerated to a normal state when there is a slight damage to organs having excellent regeneration ability, but when the damage is sustained, the liver is partially destroyed completely and the liver function is lowered, State.

Liver damage is caused by mental stress. In this case, the damaged hepatocytes need to be restored, but it is hard to find a room for resting spirit outside of modern history. In addition, due to stress, heavy drinking, It does not work and causes other diseases.

Especially, a hangover due to overeating is caused by the toxicity of alcohol and / or acetaldehyde accumulated in the hepatocyte, and the toxicity of alcohol and / or aldehyde continues for a long time, causing a disorder of metabolism and the like and causing systemic boredom, fatigue, , And vomiting.

Therefore, there is an increasing interest in compositions that have the effect of promoting alcoholysis or having a sulfation effect in order to solve the main causes that may affect liver disease.

In recent years, researches and experiments have been carried out on various functional substances for liver protection and antioxidative effects, and as a result, medicines, foods and cosmetics have been studied and marketed, but such side effects as the synthesized chemicals may be caused There are a lot of things that use the materials that are available, and it is necessary to develop various compositions containing ingredients extracted from natural foods or herbal medicine materials.

Accordingly, an object of the present invention is to provide an extract of Ganoderma lucidum, which is high in antioxidative effect, that is, a natural substance such as active oxygen, which has a high ability to remove oxidative substances that are harmful to human body, And to provide a hangover-eliminating beverage containing the same.

Another object of the present invention is to provide a cosmetic which has a high extractability against gutral exudate of natural material and an antioxidative effect containing it as an active ingredient, that is, an oxidative substance harmful to human body such as active oxygen.

The composition for a health food according to an embodiment of the present invention contains a Ganoderma lucidum extract as an active ingredient.

The extract may be water, an organic solvent or a mixed solvent thereof.

The extract may be contained in an amount of 1 to 90% by weight based on the total weight of the composition.

The food may be one of tea, beverage, meat, chocolate, food, confectionery, pizza, ramen, other noodles, gum, candy, ice cream, alcoholic beverage, vitamin complex and health supplement.

The composition may have activity to inhibit or reduce liver damage by tacrine or a substance that causes liver damage.

The composition may be antioxidant effective.

The composition for cosmetics according to another embodiment of the present invention contains a glutinous rice extract as an active ingredient.

The extract may be water, an organic solvent or a mixed solvent thereof.

The extract may be contained in an amount of 1 to 90% by weight based on the total weight of the composition.

The cosmetic may be a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, an oil, a powder foundation, an emulsion foundation, a wax foundation and a spray.

The composition may be antioxidant effective.

In addition, the present invention relates to a method for removing moisture from a baguette, Drying the cut kondan briquettes at a temperature of 30 to 35 ° C for 36 to 48 hours, pulverizing the dried kondan briquettes, extracting the crushed gondolas to prepare an extract, and extracting the extracts with beverage additives And a step of mixing the beverage at a certain ratio to prepare a beverage.

The present invention relates to a cosmetic composition or a food composition containing an extract of Mandragon beryllium as an active ingredient and a method for producing a hangover resolved beverage using the same, wherein the beverage produced by using the Mandarin extract has an antioxidative effect and liver function improving effect for suppressing hepatotoxicity , Cosmetics manufactured by using the extract of Ganoderma lucidum can be usefully used because of its antioxidative effect.

FIG. 1 is a flowchart illustrating a method for preparing a hangover solution containing the extract of Mandarin, according to an embodiment of the present invention.
FIG. 2 is a graph showing the experimental results of inhibition of tacrine liver damage by the extract of the Ganoderma Lucidum.

BRIEF DESCRIPTION OF THE DRAWINGS The above and other features and advantages of the present invention will become more apparent from the following detailed description of the present invention when taken in conjunction with the accompanying drawings, It will be possible. The present invention is capable of various modifications and various forms, and specific embodiments are illustrated in the drawings and described in detail in the text. It should be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms " comprising " or " having ", and the like, are intended to specify the presence of stated features, integers, steps, operations, elements, parts, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, parts, or combinations thereof. The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component.

Hereinafter, the present invention will be described in detail.

The present invention provides a small amount of hangover beverage having an antioxidative effect and liver function improving effect containing an extract of Ganoderma lucidum as an active ingredient.

As a material of the present invention, the above-mentioned Ligularia stenocephala (Maxim.) Matsum . & Koidz . ) Is a perennial plant that grows in many mountainous areas in Korea. The root-front leaf remains until flower is formed. It is heart-shaped, and the end is suddenly shortened. The lower part is deep deep, 24cm in length and 20cm long. There is no hairs on the surface. The back side has hairs along the vein. There are sharp sawtooth on the edge. It is 40cm long with no wings and broad base. The stem leaf is three, the bottom part is almost the same as the root lobe, the lower part of the long petiole envelopes the main stem, the upper leaf gradually becomes smaller, and the petiole becomes shorter.

The flower grows from the bottom to the bottom in August-September. It has a diameter of 2-3cm. The head of the main stem has a yellowish-headed inflorescence on its tip, and the flower has many irises. The bract is 2-3mm long and lanceolate. 3.5 cm. It has a narrow tubular shape with a length of 10-12mm, a butterfly of 2.5-3mm, 5 pieces of hairs, and a few hairs on the back. There are 1-3 or sometimes no horseshoe. The corolla is 20-25cm long and the butterfly is 3-4mm. 5-9 mm.

Achene is 6-7mm long and has a lanceolate lanceolate shape. Its tubular shape is shorter than the corolla and is white.

It is said that it is used for medicinal purposes such as stinks and for gynecological diseases in the private sector.

The present inventors have found that the above plant extract has an antioxidative effect and has an effect of inhibiting liver damage caused by, for example, alcohol or the like, which causes tacrine or liver damage, thereby protecting liver cells Respectively.

Hereinafter, a method for preparing the extract of the present invention will be described in detail.

The extracts of the present invention can be obtained by extracting and isolating the extracts from natural sources, and the extracts of the present invention are extracted from the extracts of gondolas using an appropriate solvent. For example, it includes all of crude extracts, polar solvent-soluble extracts, and non-polar solvent-soluble extracts of a mildew plant.

Preferably, it may be an extract extracted from leaves, flowers, stems, and roots of a mussel plant.

Examples of suitable solvents for extracting the above-mentioned extracts include water, organic solvents and the like, and may be, for example, purified water, methanol, Alcohol having 1 to 4 carbon atoms, acetone, ether, benzene, chloroform, and the like, including water, ethanol, propanol, isopropanol, butanol, Various solvents such as ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method.

There is no limitation on the production method of the extract, and any known method can be used.

For example, the above-mentioned first extract of the Ganoderma extract can be prepared in powder form by an additional process such as vacuum distillation, freeze-drying or spray drying. Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, the Gundalb extract is a concept including all the extracts, fractions, and purified products obtained in each step of extraction, fractionation or purification, their diluted solutions, concentrates or dried products.

A composition capable of producing a hangover-resolved beverage, which is one embodiment of the present invention, can be prepared using the extracted Ganoderma extract.

In one embodiment of the present invention, the dandelion extract of the present invention may be contained in the composition at a concentration of 10 to 100000 ppm, and the extract of the present invention may be contained in an amount of 1 to 90% by weight based on the total weight of the composition.

The composition may be a food composition containing the extract as an active ingredient. In addition to containing the active ingredient of the above-mentioned extract, the food composition may contain various flavors or natural carbohydrates as an additional ingredient ≪ / RTI >

Such natural carbohydrates include, for example, monosaccharides such as glucose, fructose, etc .; disaccharides such as maltose, sucrose, etc .; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. The above-described flavors can be advantageously used as natural flavorings (tau martin), stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.).

In addition, the food composition of the present invention may be formulated into a food composition in addition to the above-described effective ingredients, including at least one pharmaceutically acceptable or pharmaceutically acceptable carrier.

The form of the food composition may be a tablet, a capsule, a powder, a granule, a liquid, a ring, a liquid, a syrup, a juice, a suspension, an oil, or a drip agent. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methylcellulose, agar, bentonite, xanthan gum, and the like. Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile water and sterile water suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants can be additionally added to formulated into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

The food composition of the present invention formulated in the above-described manner can be used as a functional food or can be added to various foods.

Foods to which the composition of the present invention can be added include, for example, foods such as tea, beverage, meat, chocolate, food, confectionery, pizza, ramen, other noodles, gums, candy, ice cream, alcoholic beverages, .

In addition, the food composition may contain various additives such as nutrients, vitamins, minerals (electrolytes), synthetic flavors and flavors such as natural flavors, coloring agents and aging agents (such as cheese and chocolate), pectic acid and its salts , Alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks and the like. In addition, the food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks.

The active ingredient of the present invention is a natural substance, which has almost no side effects such as chemical agents. Therefore, it can be safely used for long-term administration for the purpose of antioxidative effect, alcohol decomposing ability improving effect and hepatocyte protective effect.

Accordingly, the present invention provides a health food for improving liver function, comprising the extract of Mandarin, as an active ingredient.

The health functional food of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and circles for the purpose of improving liver function such as antioxidative effect, alcohol decomposability improving effect, hepatocyte protective effect and the like.

The health functional foods of the present invention may contain conventional food additives and, unless otherwise specified, whether or not they are suitable as food additives are classified according to the General Rules for Food Additives approved by the Food and Drug Administration, Standards and standards.

Examples of the food items included in the above food additives include chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as persimmon extract, licorice extract, crystalline cellulose, high color pigment and guar gum; L-glutamic acid sodium preparations, noodle-added alkalis, preservative preparations, tar coloring preparations and the like.

Further, the dandelion extract of the present invention can be used as a cosmetic material such as a water cream, a nutritional cream, and the like.

The cosmetic composition of the present invention may contain conventional additives such as stabilizers, solubilizers, vitamins, pigments and flavoring agents, and carriers in addition to the active ingredient, Ganoderma extract.

For the purpose of antioxidant effect, the cosmetic composition may be prepared in any form conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, oils, powders A foundation, an emulsion foundation, a wax foundation and a spray, but the present invention is not limited thereto. More specifically, it can be manufactured in the form of a sun cream, a flexible lotion, a convergent lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a pack, a spray or a powder.

When the formulation is a paste, cream or gel, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component .

When the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, chlorofluorohydrocarbons, propane / Or propellants such as dimethyl ether.

When the formulation is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, - butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, a microcrystalline cellulose , Aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

A method of manufacturing a hangover-free beverage according to an embodiment of the present invention will be described in detail with reference to the drawings.

<Examples>

Referring to FIG. 1, in a method of manufacturing a hangover-solving beverage according to an embodiment of the present invention, water is removed and sliced (S10). Thereafter, it is dried at a temperature of 30 to 35 DEG C for 36 to 48 hours (S20), and the dried materials are pulverized (S30). The method of manufacturing a hangover-free beverage according to the present invention comprises the steps of: (a) preparing a bean curd refuse; (b) preparing a bean curd refuse;

The various methods of extracting the above-mentioned crushed gangbang extracts and producing them as the extracts are the same as those using the production methods of extracts in the method of producing the Kangaroobi extract, and a detailed description thereof will be omitted.

Experiments for confirming the efficacy of the dumplings according to an embodiment of the present invention and the results thereof will be described in more detail.

As an extraction method for extracting the dung beetle extract used in the hangover-free beverage manufactured to confirm the dungbean efficacy of the present invention, there are two methods of extracting hot water suitable for edible use and a 70% ethanol aqueous solution capable of extracting most active ingredients And 70% ethanol solution was fractionated into hexane, chloroform, ethyl acetate and water according to polarity.

<Experimental Example 1>

In the present invention, the content and antioxidative effect of antioxidative substances such as polyphenols, flavonoids and the like of the extract of the Ganoderma lucidum extract are measured, the alcohol decomposition ability is enhanced and the cytoprotective effect is measured. The fruit and stem of the hinoki tree containing the substance having hepatic toxicity resolution and hangover resolution activity The extracts were compared under the same conditions.

<1-1> Total polyphenol content measurement

The phenolic hydroxyl group contained in polyphenol has a function of removing active oxygen, so polyphenol is an antioxidant component.

Total polyphenol content was determined by Velioglu et al . (1998) method was measured by the extract 0.2ml, 2% sodium carbonate (Na ₂ CO ₃₎ were mixed and added to 4ml 1N Folin-Ciocalteu (FC) reagent (F9252, Sial, USA) After three minutes at room temperature 0.2 ml (Cary 4000, Varian). The absorbance at 750 nm was measured by ultraviolet ray visible spectrophotometer (Cary 4000, Varian).

Concentration (ppm) Hounsette (mg / mL) Ganoderma (mg / mL) 1000 ppm of heat 271.28 361.17 Hexane 1000 ppm 351.5 452.83 Chloroform 1000 ppm 438.5 444.28 Ethyl Acetate 1000 ppm 922.17 1168.44 H ₂ O 1000 ppm 352.61 446.22

Table 1 shows the results of the total polyphenol content of Hovenia dulcifera and Ganoderma extract. As shown in Table 1, it can be seen that the total polyphenol content of the extracts of the Ganoderma lucidum extract is higher than that of the hovenae extracts.

<1-2> Measurement of total flavonoid content

Flavonoids have antiallergic, anti-inflammatory, antioxidant, antimicrobial and anti-cancer diarrheal activity.

Total flavonoid content was measured by NFRI (1990) method. 0.4 ml of the extract, 4 ml of dimethyl glycol (111-46-6, Jun, Japan) and 0.4 ml of 1N NaOH were added and incubated at 37 ° C After reacting for 1 hour in a water bath, absorbance was measured at 420 nm. The total flavonoid content (mg · ^{- 1} ) per g of dry sample was converted to the naringin standard using a calibration curve prepared using Naringin (N0073, TCI, Japan) as a reference material.

Concentration (ppm) Hounsette (mg / mL) Ganoderma (mg / mL) 1000 ppm of heat 79.32 182.68 Hexane 1000 ppm 87.49 169.38 Chloroform 1000 ppm 92.5 108.62 Ethyl Acetate 1000 ppm 108.41 308.96 H2O 1000 ppm 82.26 114.8

Table 2 shows the results of total flavonoid content test for the Houttuynia cordata extract and the Kundalini extract. As shown in Table 2, it can be confirmed that the total flavonoid content of the extracts of the Ganoderma lucidum extract is higher than that of the hovenae.

<1-3> Measurement of antioxidative and hepatic function protection

The antioxidative effect of the extract of the present invention was confirmed by the DPPH radical scavenging activity and the ABTS radical scavenging activity method. The hepatic function coagulation effect was measured by ADH enzyme activity assay, hepatocyte protective effect using HepG2 hepatocyte and tacrine . In the present invention, the activities of the Houttuynia cordata extract and the Gondwabi extract were respectively measured and compared.

<1-3-1> DPPH radical scavenging activity

1 ml of each concentration extract and 4 ml of 0.15 mM DPPH (1,1-diphenyl-2-picrylhydrazyl; D9132, Aldrich, USA) (Molyneux, 2004) were mixed and reacted for 30 minutes at room temperature and darkness. (Blois, 1958). (EDA) was calculated by the following equation (1) as the percentage of absorbance difference between sample addition and no sample addition. The simple regression analysis showed that the concentration of the sample required to reduce EDA by 50% mg · ^-1 ) was expressed as RC ₅₀ value. As a positive control, L-ascorbic acid (50817, Aldrich, USA) was used.

Electron donating ability (EDA,%) = [(control-sample) / control] × 100 Equation (1)

Table 3 above shows the results of DPPH radical scavenging test of Hovenia dulcifera and Ganoderma lucidum extract.

DPPH radical scavenging activity of Hovenia dulcis extracts and Ganoderma lucidum extracts was found to be electron donating ability from 800 ppm to 24.98% and from 1,000 ppm to 13.58% in hydrothermal solution. In other cases, it was found that the extract of Ganoderma lucidum generally had better electron donating ability than that of the hound extract.

<1-3-2> ABTS radical scavenging activity

The total antioxidant activity of ABTS radicals was measured by using 7.4 mM ABTS and 2.6 mM potassium persulfate, using both water-soluble and lipid-soluble antioxidants. The method was relatively simple and sensitive (Pellegrini et al ., 1999) Were mixed and allowed to stand at room temperature and in a dark place for 24 hours to form radicals. The ABTS solution was diluted with PBS (pH 7.4) to an absorbance of 0.70 (mean ± 732 nm) at 732 nm, and 950 μl of ABTS solution was added to 50 μl of the extract by concentration. absorbance was measured at. (Blois, 1958;. Osman et al, 2006) ABTS radical scavenging activity (RC ₅₀₎ was obtain in the same way as the DPPH radical scavenging activity, the concentration of the soluble solid required to reduce the absorbance of the control group and 50% ( mg · ^-1 ). L-ascorbic acid was used as a positive control to compare the radical scavenging ability.

Table 4 shows the results of the ABTS radical scavenging activity test of the hound extract and the rumen extract.

The results of the ABTS radical scavenging activity test of the extracts of Hovenia dulcifera and Gondorvii showed that the hydrothermal activity increased from 200 ppm to 17%, the hydrothermal activity increased to 5% at 300 ppm and the activity of hydrothermal activity was 26% Respectively. In other cases, it was generally found that the extract of Ganoderma lucidum was superior to the extract of Hovenia dulcis.

<1-3-2> Measurement of alcohol dehydrogenase (ADH) activity

The ADH enzyme activity was measured by the modified method of Choi et al. And Racker. The absorbance of the produced NADH was measured at 340 nm. Add 0.1 mL of alcohol, 0.5 mL of NAD aqueous solution (2 mg / mL) and 0.1 mL of the sample to the test tube, and adjust the volume to 1.8 mL with 10 mM glycine-NaOH buffer (pH 8.8) The reaction was carried out for 10 minutes in a 25 ° constant-temperature water bath, and the change in absorbance at 340 nm was measured by adding ADH (10 unit / mL).

Table 5 above shows the test results of the ADH enzyme activity measurement of the Houttuynia cordata extract and the Gondweed extract.

The ADH enzyme activities of the extracts of Hovenia dulcis and Ganoderma lucidum were determined to be 400% at 80 ppm and 154% at 80 ppm in hydrothermal solution. In other cases, it was generally found that the extract of Ganoderma lucidum was superior to the extract of Hovenia dulcis.

<Experimental Example 2>

Experiments were conducted to determine whether inhibition of hepatocyte damage by tacrine substances damaging hepatocytes was induced by using the extract of Ganoderma lucidum according to one embodiment of the present invention.

The hepatocytes used in the experiments were cultured in MEM medium supplemented with 10% heat-inactivated fetal bovine serum and 100 U / mL penicillin and 100 μg / mL streptomycin at 37 ° C. , Hepatocyte Hep G2 cultured in a 5% CO ₂ incubator was used.

The experimental groups were as follows: control group (C) containing only hepatocytes, tacrine group (Tacrine) cultured with hepatocytes and tacrine, and 10 μg / mL of each group of hepatocytes and tacrine- 50 μg / mL, 100 μg / mL, and 200 μg / mL were incubated at the concentration of 0.15 mM for 24 hours and 48 hours.

After removing the MEM medium, 100 μL of MTT reagent (5 mg / mL in PBS) was added and incubated in a carbon dioxide atmosphere at 37 ° C. for 2-3 hours. After confirming that black spots were formed, the MTT reagent was removed, Were added and the mixture was mixed for 15-20 minutes. Then, the absorbance was measured at 540 nm to determine the survival rate of the cells.

24 h C Tacrine 10 50 100 200 2.5146 1.9829 2.1184 2.1832 2.2235 2.563 Absorbance 2.1423 1.8016 1.8544 1.7813 1.884 2.1009 2.1773 1.9634 1.974 1.9923 1.9563 2.3099 Average 2.278067 1.915967 1.982267 1.9856 2.021267 2.3246 Cell viability (%) 100 84.10494 87.01531 87.16163 88.72728 102.0427 Standard Deviation 0.20559 0.099523 0.132194 0.201034 0.178831 0.2314 Standard deviation percentage 9.024759 4.368756 5.802903 8.824753 7.850126 10.15776 48 h C Tacrine 10 50 100 200
Absorbance
1.8638 0.9825 1.4899 1.602 1.5703 1.6974 1.6291 1.1008 1.3873 1.4999 1.3963 1.7712 1.8637 1.0138 1.4983 1.405 1.6027 1.6095 Average 1.785533 1.032367 1.4585 1.5023 1.5231 1.6927 Cell viability (%) 100 57.81839 81.68428 84.13733 85.30224 94.80081 Standard Deviation 0.135475 0.061297 0.061804 0.098522 0.111001 0.080952 Standard deviation percentage 7.587383 3.432953 3.461368 5.517787 6.21666 4.533793

Table 6 shows the results of the test for inhibition of tacrine liver damage by the extract of Ganoderma lucidum, and FIG. 2 is a graph showing the results of the test for inhibition of tacrine liver damage by the Ganoderma extract.

As shown in Table 6 and FIG. 2, it can be seen that the absorbance of cells treated with the extract of Ganoderma lucidum is higher than that of cells cultured only with hepatocytes and tacrine, and the higher the concentration, the higher the absorbance and the survival rate of hepatocytes Thus, it can be seen that the effect of the extract of Ganoderma lucidum against hepatocyte injury was confirmed.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention.

Claims (12)

An antioxidant effect containing an extract of Ganoderma lucidum as an active ingredient and a composition for food for improving liver function. The method according to claim 1,
Wherein the extract is water, an organic solvent, or a mixed solvent thereof. The method according to claim 1,
Wherein the extract is contained in an amount of 1 to 90% by weight based on the total weight of the composition. The method according to claim 1,
Wherein the food is selected from the group consisting of tea, beverage, meat, chocolate, foodstuff, confectionery, pizza, ramen, other noodles, gums, candy, ice cream, alcoholic beverages, vitamin complexes and health supplements. The method according to claim 1,
Wherein said composition has an activity of inhibiting or reducing liver damage by tacrine or a substance that causes liver damage. The method according to claim 1,
Wherein the composition has an antioxidant effect. A composition for cosmetics for antioxidative effect containing an extract of Ganoderma lucidum as an active ingredient. 8. The method of claim 7,
Wherein the extract is water, an organic solvent, or a mixed solvent extract thereof. 8. The method of claim 7,
Wherein the extract is contained in an amount of 1 to 90% by weight based on the total weight of the composition. 8. The method of claim 7,
Wherein the cosmetic is selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, oils, powder foundations, emulsion foundations, wax foundations and sprays. 8. The method of claim 7,
Wherein the composition has an antioxidant effect. Washing the gondolas, removing moisture and thinly cutting the gondolas;
Drying the cut sandwich at a temperature of 30 to 35 DEG C for 36 to 48 hours;
Crushing the dried khonatan;
Extracting the pulverized sandalwood to produce an extract; And
And mixing the extract with the beverage additives at a predetermined ratio to prepare a beverage.

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