KR101444879B1 - Moisturizer compositions containing extracts of crude drug - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin wrinkles, and more particularly, to a cosmetic composition comprising a natural herbal medicine extract. In addition, the present invention has the effect of enhancing the skin moisturizing power by promoting the synthesis of hyaluronic acid by including the herbal medicine extract in the cosmetic, and inhibiting the synthesis of the tissue degrading enzyme of the skin to improve the skin wrinkles.

Description

[0002] Moisturizer compositions containing extracts of crude drugs

The present invention relates to a cosmetic composition for improving skin wrinkles, which is capable of enhancing the lipid barrier function by promoting the synthesis of aged skin lipid components, improving the resilience of a damaged skin barrier, and also having an excellent moisturizing and homeostatic maintenance effect.

The skin consists of three layers and subcutaneous tissues such as stratum corneum, epidermis, and dermis from the outside. The double stratum corneum (SC) is very important because it directly touches the external environment. The stratum corneum contains 10 to 30% of water, and functions not only to suppress the outflow of moisture, electrolytes, and the like but also to function as a barrier to prevent foreign matter from penetrating from the outside. In addition, the stratum corneum, which maintains an adequate moisture content, provides elasticity and flexibility to the skin and allows the skin to function normally. Moisturizing power, which is an important factor in skin aging, is controlled by the epidermis. The stratum corneum of the epidermis is an accumulation of dead cells, but plays a very important role in maintaining the moisture of the skin. The amount of moisture in the stratum corneum produced by regular keratinization is always 10-20%. Therefore, transepidermal water loss, which is an index of stratum corneum damage, is increased, which is also the cause of primary inflammation reaction. For this reason, conventional cosmetics have a problem that harmful side effects are generated on the skin, and thus there is a limit to obtaining skin moisturizing effect and protective effect.

The epidermis, dermis, and hyaluronic acid present in each layer play an important role in skin aging as well, and the amount of hyaluronic acid decreases with aging, resulting in wrinkles. That is, hyaluronic acid is produced in keratinocytes and plays an important role not only in hydration of the stratum corneum but also in the regulation of water retention and water and ion exchange. Hyaluronic acid, which is an anti-aging material and also called a natural moisturizer, helps the collagen in the skin to work well between the cells and promotes the transport of ions and nutrients important for skin homeostasis. Gives a sense of volume and elasticity. In contrast to collagen, it has a short life span. As you get older and your hyaluronic acid in the skin gets smaller, the skin is dry, elastic and wrinkle-like.

In addition, as the skin ages, the internal factors cause various imbalances of hormones related to metabolism. As external factors, free radicals and active oxygen due to ultraviolet rays are increased, and skin thickness is reduced and wrinkles are generated. The action is deteriorated.

Also, as the skin ages, the contents of collagen, elastin, hyaluronic acid and glycoprotein, which are skin constituents, decrease and oxidative stress is applied. The biosynthesis of cytooxygenase-2 (Cox-2), an enzyme that produces proinflammatory cytokine by ultraviolet rays, is increased and matrix metalloprotease (enzyme), which is an enzyme that decomposes skin tissue by these inflammatory factors MMP, Matrix metalloproteinase) and nitric oxide (NO) production by iNOS (inducible nitric oxide synthase). In other words, it decreases the biosynthesis of the substrate material due to the decrease of cellular activity and inflammation due to natural aging, the pathway of lipid peroxidation due to the oxidative stress caused by the external environment, and the lipid peroxide accumulates, resulting in functional impairment, A phenomenon occurs. It is urgent to develop antioxidants such as compounds capable of scavenging free radicals or substances inhibiting peroxidation as aging and inhibition or treatment of various diseases.

In recent years, natural cosmetics have been increasingly demanded due to naturalism and well-being trends. To solve this problem, a variety of methods for producing an extract have been developed, and functional foods and cosmetics using natural extracts have been developed. However, development of a natural cosmetic composition or a raw material thereof having an excellent moisturizing effect and an excellent wrinkle-improving effect on skin has not yet been developed.

Korean Patent Laid-Open No. 10-2009-0015127 (Published Date: February 11, 2009) Korean Patent Publication No. 1998-027404 (published on July 15, 1998)

The present invention provides a cosmetic composition containing a natural medicinal plant extract having excellent moisturizing effect and excellent skin wrinkle-improving effect as a natural cosmetic composition for solving the above-mentioned problems.

In order to attain the above object, the present invention provides a moisturizing composition comprising mashish extract, ginseng extract and ginsenoside Rh1.

According to a preferred embodiment of the present invention, ginsenoside Rh1 may be extracted from ginseng.

According to another preferred embodiment of the present invention, the Ganoderma lucidum extract and the ginseng extract may be respectively extracted with at least one solvent selected from the group consisting of water and C ₁ -C ₄ alcohols.

According to another preferred embodiment of the present invention, the moisturizing composition of the present invention may comprise 40 to 100 parts by weight of ginseng extract and 5 to 20 parts by weight of ginsenoside Rh1 with respect to 100 parts by weight of the ginseng extract.

According to another preferred embodiment of the present invention, the moisturizing composition of the present invention comprises at least one member selected from the group consisting of oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a pigment, And may further include additives.

In addition, the present invention provides a cosmetic composition for improving skin wrinkles comprising a mashin extract, ginseng extract and ginsenoside Rh1.

According to a preferred embodiment of the present invention, ginsenoside Rh1 may be extracted from ginseng.

According to another preferred embodiment of the present invention, the Ganoderma lucidum extract and the ginseng extract may be respectively extracted with at least one solvent selected from the group consisting of water and C ₁ -C ₄ alcohols.

According to another preferred embodiment of the present invention, the cosmetic composition for improving skin wrinkles according to the present invention comprises 1 part selected from the group consisting of oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a pigment, And more than one kind of additive.

According to another preferred embodiment of the present invention, the cosmetic composition for improving skin wrinkles according to the present invention is one or more selected from the group consisting of cosmetics, essences, skins, lotions, creams, packs, Lt; / RTI >

The term " ginsenoside "of the present invention refers to saponin present in ginseng, and the saponin is a compound chemically called glycoside.

The "extraction processing" of the present invention includes a step of performing simple extraction using a solvent or processing after the simple extraction.

The present invention provides a cosmetic composition which is excellent in moisturizing effect and wrinkle-reducing effect, does not cause side effects such as skin irritation, is excellent in promoting biosynthesis of hyaluronic acid, and has excellent skin moisturizing activity.

FIG. 1 is a graph showing the inhibitory effect of MMP-1 on the extracts prepared in Preparation Examples 1 to 2 and Example 1 in Experimental Example 2. FIG.

The present invention provides a moisturizing composition comprising horseradish extract, ginseng extract and ginsenoside Rh1.

The ginsenoside Rh1 is not particularly limited as long as it is normally cultivated and sold, but it may be one extracted from ginseng. The method of extracting ginseng is not particularly limited as long as it is a method of extracting a natural extract, Preferably one or more solvents selected from the group consisting of water and C ₁ to C ₄ alcohols. More preferably methanol or ethanol, but the present invention is not limited thereto.

The processing is not particularly limited as long as it is a method for processing a natural extract, but chromatography can be carried out, but the present invention is not limited thereto.

The mashin extract and the ginseng extract are not particularly limited as long as they are usually extracted with natural extracts. Preferably, the extract is extracted with at least one solvent selected from the group consisting of water and C ₁ -C ₄ alcohols More preferably methanol or ethanol, but the present invention is not limited thereto.

The moisturizing composition of the present invention is not particularly limited as long as it contains ginseng extract, marigold extract and ginsenoside Rh1, but preferably 40 to 100 parts by weight of ginseng extract per 100 parts by weight of ginseng extract and 5 to 20 parts by weight of ginsenoside Rh1 Parts by weight.

The moisturizing composition of the present invention is not particularly limited as long as it is added to the moisturizing composition. The moisturizing composition of the present invention can be added to the moisturizing composition. Preferably, the moisturizing composition includes oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, And an additive including at least one member selected from the group consisting of

Furthermore, the moisturizing composition of the present invention can be prepared in various formulations and can be applied to a conventional method for producing a moisturizing composition. Preferably, the moisturizing composition is in the form of cosmetics, essences, skins, lotions, creams, packs, Or a combination thereof.

In addition, the present invention provides a cosmetic composition for improving skin wrinkles comprising a mashin extract, ginseng extract and ginsenoside Rh1.

The ginsenoside Rh1 is not particularly limited as long as it is cultivated and sold in general, but may be extracted from ginseng. The method of extracting ginseng is not particularly limited as long as it is a method of extracting a natural extract, Preferably one or more solvents selected from the group consisting of water and C ₁ to C ₄ alcohols. More preferably methanol or ethanol, but the present invention is not limited thereto.

The processing is not particularly limited as long as it is a method for processing a natural extract, but chromatography can be carried out, but the present invention is not limited thereto.

In addition, each of the mashii extract and the ginseng extract is not particularly limited so long as it is a method for extracting natural extracts. Preferably, the extract is extracted with at least one solvent selected from the group consisting of water and C ₁ to C ₄ alcohols More preferably methanol or ethanol, but the present invention is not limited thereto.

The cosmetic composition for improving skin wrinkles of the present invention is not particularly limited as long as it is added to the cosmetic composition in general, but it may be added in the form of oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, And an additive comprising at least one member selected from the group consisting of flavoring agents, and the like.

Further, the cosmetic composition for improving skin wrinkles of the present invention can be prepared in various formulations and can be applied to a conventional method for producing cosmetic products. Preferably, the cosmetic composition can be used in cosmetics, essences, And make-up bases.

Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are intended to further illustrate the present invention, and the scope of the present invention is not limited to these examples.

[ Example ]

Preparation Example  One. Machi  Extract preparation

100 g of mashimyeon were finely crushed in a crusher and immersed in 400 ml of 50% ethanol, and the mixture was warmed in a 60 ° C water bath for 2 hours or more while emitting ultrasonic waves. The obtained extract was cooled to room temperature and filtered with filter paper to obtain a filtrate. After two repetitions, the mixture was concentrated under reduced pressure and then freeze-dried to obtain 35 g of a crude extract.

Preparation Example  2. Manufacture of ginseng extract

100 g of commercially available ginseng was finely pulverized in a crusher, immersed in 400 ml of 75% ethanol, and warmed in a 60 ° C water bath for 2 hours or more while emitting ultrasonic waves. The obtained extract was cooled to room temperature and filtered with filter paper to obtain a filtrate. After 2 repetitions, the mixture was concentrated under reduced pressure and then lyophilized to obtain 21 g of ginseng extract.

Example  1. Preparation of crude drug mixture

1 g of the gingival extract prepared in Preparation Example 1, 2 g of the ginseng extract prepared in Preparation Example 2 and 0.2 g of Ginsenoside Rh1 (KAIST, Invitrogen Corporation) were mixed well with a stirrer to prepare a crude drug mixture.

Comparative Example  One.

1 g of the crude extract prepared in Preparation Example 1 and 2 g of the ginseng extract prepared in Preparation Example 2 were mixed well with a stirrer to prepare a crude drug mixture.

Experimental Example  1. Measurement of synthesis effect of hyaluronic acid

Hyaluronic acid is a cement-like viscosity material that fills the cell gap and plays an important role in skin elasticity and moisture retention of the epidermis. The increase in the synthesis of hyaluronic acid in the treatment of ginseng complex extract was confirmed by hyaluronic acid measurement test after treating the cells.

Keratinocytes were cultured and cultured in KSFM medium at 37 ° C and 5% CO ₂ for 24 hours. 0.5% or 1% of each of the extracts of Preparations 1 and 2, the herb medicine mixture of Example 1, the ginsenoside Rh1 or the herb medicine mixture of Comparative Example 1 was added to the medium and cultured for 72 hours. The supernatant was taken at -80 ° C . The amount of hyaluronic acid in the supernatant was measured using an ELISA assay kit (Biogenic Cat. No. 029001). The amount of the measured hyaluronic acid is shown in Table 1 below.

division Hyaluronic acid (占 퐂 / ml) Hyaluronic acid (%) Untreated group 2.9 ± 2.62 100 Ginsenoside Rh1 3.12 ± 2.64 107.58 The crude extract of Preparation Example 1 2.79 ± 0.18 90.06 Preparation Example 2 Ginseng Extract 2.81 ± 0.13 99.29 The crude drug mixture of Comparative Example 1 2.88 + 0.04 103.23 The crude drug mixture of Example 1 3.48 0.10 120.83

As shown in Table 1, it was confirmed that the amount of hyaluronic acid present in the culture supernatant when treated with 10 μg / ml of the herbal drug mixture of Example 1 was increased by 20% or more as compared with the non-treated group to which no extract was added . In addition, it was confirmed that much more hyaluronic acid was produced in the cells treated with the crude drug mixture of Example 1 than the cells treated with the crude drug mixture of Comparative Example 1, which is a mixture of the mashii extract and the ginseng extract. These results show that the herbal preparation mixture of Example 1 has excellent moisturizing effect.

Experimental Example  2. Wrinkle improvement effect measurement

Human fibroblast (HaCaT) was cultured in a 12-well incubator at a concentration of 10 ⁵ , and then irradiated with ultraviolet ray B at 40 mJ / cm ^{2. Then} , the herbal drug mixture prepared in Example 1, the extract of Preparation Examples 1 and 2, Side Rh1 or TGF-? As a positive control was replaced with medium treated with 1 占 퐂 / ml or 10 占 퐂 / ml. On the second day of culture, the cells were harvested and the amount of MMP-1 (matrix metalloproteinase I) produced by ELISA was quantified. The result was calculated as a comparison value with a value measured with 100 as a UV-untreated control group, and TGF-β was used as a positive control group. The inhibitory effect of the measured matrix metalloproteinase-1 (MMP-1; Matrix metalloproteinase-1) expression is shown in FIG.

1, in the human normal fibroblast monolayer culture system, the crude drug mixture of Example 1 according to the present invention inhibited the expression of MMP-1 induced by 40 mJ / cm ² of UVB (ultraviolet B) I could confirm. In addition, it was confirmed that the expression level of MMP-1 was the lowest in the cells treated with the crude drug mixture of Example 1 than the cells treated with the ginsenoside Rh1, phosphate extract or horseradish extract. These results show that the herbal drug mixture of Example 1 inhibits the biosynthesis of MMP-1, a skin tissue degrading enzyme that occurs after irradiation with ultraviolet light belonging to external environmental factors, and is effective in suppressing skin aging and improving wrinkles .

Experimental Example  3. Skin Moisture power  evaluation

The following method was used to evaluate the skin moisturizing power of the herbal medicine mixture of Example 1 dissolved in distilled water and diluted to a concentration of 10%.

And the amount of the diluted herbal medicine mixture was applied to the skin, and then measured using a moisture meter. As shown in Table 2, measurements were taken 5 times immediately before the first application, at 3:00 pm on the first day, 5:00 pm on the first day, 3:00 pm on the third day, and 5:00 pm on the third day.

1 day 2 days
Apply 3 times a day
(9 o'clock, 16 o'clock,
23 o'clock) 3 days Measure 1 Measure 2 Measure 3 Measure 4 Measure 5 Before application 4 hours after first application 8 hours after first application 2pm 5pm Measured with a moisture meter Measured with a moisture meter

The width was measured to be 9cm ^2. The measured value was measured by pressing the measuring device closely to the surface of the skin of a specific area and then lightly pressed. The measured value was the average value of the measurements of each subject. The measured values are shown in Table 3 below.

division Measure 1 Measure 2 Measure 3 Measure 4 Measure 5 Skin moisture average measure 42 49 48 51 53

After 3 days of application, the average value of the skin moisture content of the subject increased. Measurement 1 was measured before the first application, and skin moisture was remarkably increased when 4 hours after the first application and after measurement 2. In addition, when the measurement was performed 8 hours after the first application, the water content of the skin was kept relatively high as compared with that of the measurement 1, showing the continuous efficacy of the diluted pharmaceutical preparation mixture. It is also found that when the diluent is applied periodically over several days, the amount of skin moisture is gradually improved.

As can be seen from the above Experimental Example, the crude herbal medicine mixture of the present invention is a natural extract having a continuous moisturizing effect and excellent wrinkle-reducing effect, and can be used as a cosmetic composition which is effective in improving the moisture content of the skin and improving the wrinkles of the skin I could confirm.

Claims (11)

40 to 100 parts by weight of ginseng to 100 parts by weight of ginseng extract and 5 to 20 parts by weight of ginsenoside Rh1. The moisturizing composition according to claim 1, wherein the ginsenoside Rh1 is extracted from ginseng. The method of claim 1, wherein the Portulaca Oleracea extract and ginseng extract is moisturizing composition, characterized in that the extraction with water, and each C ₁ ~ one or more solvents selected from the group consisting of alcohols of C _4. delete 4. The composition according to any one of claims 1 to 3, wherein the moisturizing composition includes at least one member selected from the group consisting of oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a pigment, ≪ / RTI > wherein the composition further comprises an additive. A cosmetic for improving skin wrinkles, which comprises the moisturizing composition of any one of claims 1 to 3. delete delete delete delete [7] The cosmetic composition for improving skin wrinkles according to claim 6, wherein the cosmetic for improving skin wrinkles is any one selected from the group consisting of cosmetics, essences, skins, lotions, creams, packs, Cosmetics for improving wrinkles.

Images