KR101439102B1 - New process technology for effective manufacture of ginseng berry extraction and concentration - Google Patents

Abstract

The present invention relates to a method for producing ginseng berry extracted and concentrated liquid, wherein ginsenoside Re and Rd which are the standard of ginseng berry`s pharmacological active ingredients are not destroyed, and a process yield is increased. A liquor solution which is an extraction solvent containing ginseng berries which are raw ingredients is firstly extracted while an inert gas is injected therein, and concentrated so that first extracted and concentrated liquid and a first extracted residue are collected. The first extracted residue is secondly extracted and concentrated using alpha-amylase, which is a starch degrading enzyme, and ultrasonic wave injection, so that second extracted and concentrated liquid is collected. A final product is produced by mixing and concentrating the first and second extracted and concentrated liquids. In the present invention, extraction is performed while the ginsenoside Re and Rd contained in the ginseng berries are not destroyed, and medicinal effects of the ginseng berries are conserved. A production procedure is simple, and a production unit cost can be reduced, so that the same is highly competitive in a health functional food ingredient, medical product ingredient, or cosmetic ingredient market, and a consumer can be provided with a high quality and low price product.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel process for the production of ginseng berry extract and concentrate,

The present invention relates to a method for producing a juice extract concentrate, and more particularly, to a method for efficiently producing a juice extract concentrate without destroying an effective ingredient by applying a juice extract process.

Grape berry is a botanical fruit of plants belonging to Araliaceae and Panax, and has been used as a medicinal product of Donggwoogam along with red ginseng that has been used for thousands of years as a mysterious herb. Jinsen-no-saeed is a pharmacologically active ingredient contained in Ginseng Berry. It is used for cell regeneration, anti-inflammation, central nervous system diseases, anxiety, nervous system, drug addiction detoxifying effect, vascular action, alcohol detoxification, lipid metabolism and fatigue recovery, In particular, panaxadiol (PD) saponin represented by ginsenoside Rb ₁ has an inhibitory action on the central nervous system, and thus is effective for mental stability, nervous relaxation, analgesia , Panaxatriol (PT) saponins represented by ginsenoside Rg ₁ are known to exhibit anti-fatigating action by acting on the central nervous system [Latest Korean Ginseng Research, Korea Ginseng Society, 2007].

The content of ginsenosides contained in ginseng berry is ginsenoside Rg _{1 1} ~ 2 wt%, Re 28 ~ 40 wt%, Rf 1 ~ 8% by weight, Rb ₁ 6 ~ 7% by weight, Rg ₂ 5 ~ 14 wt. % Of Rc, 2 to 3.6% by weight of Rc, 10 to 11% by weight of Rb ₂ , 1 to 2% by weight of Rb ₃ and 16 to 33% by weight of Rd. Particularly, And 40% to 73% by weight of the ginseng root.

Due to the above-mentioned reasons, the extract concentrate of Ginsengberry has excellent potential for anti-aging, anti-inflammation and cell regeneration, and has great industrial potential for the treatment of cosmetics, hair follicles and arthritis. , The demand is rapidly increasing. However, various ginsenosides contained in Ginseng Berries are converted into different types of ginsenosides by converting the molecular structure according to the conditions of various manufacturing processes applied during extraction concentration Or, it is completely decomposed and transformed into an organic matter that has no efficacy, and the inherent efficacy of the fresh berries is lost.

Generally, the production technology for producing high-quality raw material without destroying the inherent active ingredient of Jinju berry in the manufacturing process and the manufacturing technique for improving the production yield are contradictory to each other. For example, in the red ginseng concentrate process In order to prevent the active ingredient of red ginseng from being destroyed, it is necessary to extract and concentrate at low temperature, do not use a polar solvent and acid, and work to exclude pressurization and harsh conditions during extraction and concentration, It is general to apply the opposite condition to the above. In addition, generally, a natural circulating concentrate concentrate is used to prepare a juice extract concentrate. This method is difficult because of the bubbles caused by saponin (ginsenoside), an effective ingredient of red ginseng, There is a drawback in that it is long, and there is a problem of using a silicone-based synthetic antifoaming agent to solve this problem.

As a prior art related to the process for producing extractive concentrate of juvenile berry, for example, in Korean Patent Registration No. 10-1051519, an enzyme such as alpha-galactosidase, pectinase, cellulase and lactase is used to produce ginseng fruit Discloses a method for extracting ginseng fruit using yeast, mushroom germ, lactic acid bacterium, ginseng, and bacillus ginseng in Korean Patent Laid-Open Publication No. 10-2011-0098122. And Korean Patent Laid-Open No. 10-2011-0098123 discloses a method for producing ginseng fruit extract and composition thereof by salting-out fermentation method. In Korean Patent Laid-Open Publication No. 10-2013-0085576, Which is then pickled and placed in an ongyo which is shielded from sunlight together with the koji enzyme solution to produce a ginseng fruit aging solution, and disclosed in Korean Patent Publication No. 10-2013-0 029866 discloses a method for preparing ginseng fruit oil using a supercritical fluid. In Korean Patent Laid-Open Publication No. 10-2013-0074118, ginsenosides Rg ₂ , Rh ₁ , F _4, etc., Discloses a method for producing an extract of ginseng fruit containing a high concentration and Korean Patent Laid-Open Publication No. 10-2011-0078525 discloses a method for preparing ginseng fruit extract by adding 1 L of water to 1 kg of dried ginseng fruit and concentrating it by simple reflux extraction Lt; / RTI >

The above prior arts have developed extractive concentration techniques such as fermentation, ultrasonic, supercritical, etc. for the purpose of structural conversion into ginsenosides having specific effects, but the physiologically active components of the extract solution There is no manufacturing process patent developed to enable high efficiency production.

In order to solve the problems of the prior art described above, the present invention relates to a process for extracting fresh juice berry using an inert gas, an ultrasonic wave and a starch decomposing enzyme to produce a novel juice berry extract Thereby completing the present invention.

The present invention aims to provide a novel process for efficiently producing a jujube berry extract concentrate from jujube berry. More specifically, the present invention relates to a novel process for producing a jujube berry extract concentrate containing 40 to 73% The present invention is intended to provide a method for producing a jujube berry extract concentrate which is preserved so that the components ginsenosides Re and Rd are not destroyed (converted) and extracted at a high yield.

As a solution for achieving the object of the present invention, there is a method for preparing a juice extract concentrate, in which an inert gas is uniformly injected into the interior of a juice solution, which is an extraction solvent containing a raw juice, After concentrating and recovering the primary extract and the primary extract, the primary extract was subjected to secondary extraction using alpha-amylase, a starcholytic enzyme, and ultrasonic injection, followed by concentration, followed by recovery of the secondary extraction concentrate, 1 < / RTI > and a second extraction concentrate to produce the final product.

As shown in [Figure 1] and [Figure 2], the extraction process (technology) is generally known as a variety of extraction processes (techniques) such as fermentation treatment, acid treatment, high temperature treatment or long- , The various ginsenosides and the main pharmacological components Re and Rd of the ginseng berry are converted into other ginsenosides whereas the other ginsenosides are not converted into Re and Rd And the process yield (extraction yield) is remarkably lowered when a low-temperature treatment and a short-time process condition are applied in order to prevent the preservation (conversion) (destruction) of the ginsenosides Re and Rd [Latest KOREA Ginseng Research , Korea Ginseng Society, 2007].

The method of preparing the juice extract concentrate according to the present invention is characterized in that the juice solution as an extraction solvent and nitrogen as an inert gas are suitably applied to inactivate the raw material mixture to thereby contain 40 to 73% (Conversion) of the ginsenosides Re and Rd which are contained in the ginsenosides Re and Rd.

[Figure 1]

[Figure 2]

More specifically, the present invention provides a method for producing a concentrated juice extract concentrate comprising the steps of: a) extracting an inert gas into a raw material mixture containing a raw material berry and a solvent containing an alcohol, A first extraction step of recovering the first extraction concentrate by concentrating the first extraction liquid after separation into the first extraction residue, and b) a step of extracting the first extraction residue separated in the first extraction step with the purified water and the starcholytic enzyme alpha - A second extraction step in which amylase is added and second extraction is performed while injecting an ultrasonic wave to separate into a second extraction solution and a second extraction residue and then the second extraction solution is concentrated to recover a second extraction concentrate and c) And a final extraction concentrate recovery step of mixing the first and second extraction concentrates recovered in the extraction step to obtain a final extraction concentrate.

A) According to the present invention, the raw material berry of the first extraction step is not limited to the kind of the region / year, but it is preferable to use the berry of more than 4 years old. The extraction solvent containing 10% by weight to 90% by weight of the alcohol is used as a mixture of alcohol and purified water, and the amount of the extraction solvent used is the weight of the raw material berry weight This is done by using twice the contrast.

A) Injection of an inert gas into the raw material mixture in the first extraction step prevents the raw material mixture from being inactivated by gas-liquid contact between the raw material mixture and the inert gas during the extraction process to prevent destruction (conversion) of ginsenosides Re and Rd And it is preferable to remove oxygen in the air contained in the raw material mixture before the inert gas is injected. This is because the raw material mixture is inactivated by the inert gas and the destruction (conversion) of ginsenosides Re and Rd The inert gas is preferably an argon gas or a nitrogen gas. In view of economics, nitrogen is preferred.

Nitrogen gas is injected into the inert gas by repeating injection and stoppage at intervals of 30 minutes from the bottom of the raw material mixture so that bubbles are generated. Ml / min and the mixture is extracted at a temperature ranging from 65 ° C to 75 ° C for 7 to 10 hours.

In addition, a) the first extraction residue separated in the first extraction step is used as a raw material for the second extraction step, and the separated first extraction solution is subjected to extraction at a temperature of 140 to 160 ° C and 1 to 10 mmHg in a short time, (Falling Film type), and concentrating the mixture to obtain a first concentrated concentrate.

The b) secondary extraction step according to the present invention is characterized in that the primary extraction residue contains a raw material berry Two times as much weight of purified water was added, and alpha-amylase was added to the raw juice berry 0.1 to 1.4% by weight based on the weight of the extract, and the extraction is carried out at 75 to 85 ° C. During the extraction, ultrasound at 30 kHz is injected for 1 to 8 hours while being injected. B) The starch of α-amylase, which is an enzyme, is decomposed to improve the extraction yield and the starch decomposition of α-amylase is activated by the injection of ultrasound. .

B) Secondary extraction from the second extraction step is also concentrated by falling-film type (Falling Film type) concentration in a short time at a temperature of 140 to 160 ° C and 1 to 10 mmHg, To obtain a concentrated liquid.

The first extraction concentrate and the second extraction concentrate are mixed with each other to finally produce a final extract concentrate according to the present invention, and the second extraction residue is discarded.

The method for preparing a juice extract concentrate according to the above-mentioned a) first extraction step to c) the final extraction concentrate recovery step of the present invention is characterized in that the extract is inactivated using an inert gas and the main pharmacological component The total amount of the ginsenosides Re and Rd is extracted in the first stage while being prevented from being destroyed, and at the same time, the ginsenosides Re and Rd are almost completely extracted And further extracting ginsenoside, which is a pharmacological ingredient contained in the extracted residue, with alpha-amylase and ultrasonic waves to further improve the yield of the active ingredient.

The process for the preparation of the concentrated juice extract concentrate according to the present invention is an efficient process for preparing an extract concentrate in a simple and short time, and the extracted concentrate of the juice berry is contained in the juice berry in an amount of 40 to 73% by weight It is characterized in that it is made from a jujube berry extract concentrate with a higher process yield while preventing the ginsenosides Re and Rd from destroying (converting) the pharmacological efficacy components.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a process diagram schematically showing a method for producing a jujube berry extract concentrate according to the present invention
2 is a graph showing the ginsenoside analysis data (A) of the fresh juice extract concentrate according to the present invention and the ginsenoside analysis data (B) of the fresh juice extract concentrate obtained under the conditions of the conventional process

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, and the present invention is not limited by the following description, and the units of the parts and percentages used in the present invention are all expressed by weight will be.

In the examples and test examples of the present invention, ginsenoside Re And Rd were prepared according to the preprocessing method of food circulation, and then analyzed by HPLC (High Performance Liquid Chromatography) equipped with UVD (Ultra Visible Detector), as shown in [Table 1] and [Table 2] Were analyzed by the same HPLC analysis conditions and the equation of ginsenoside content.

In the examples and test examples of the present invention, the content of benzopyrene was analyzed by HPLC (High Performance Liquid Chromatography) equipped with a FLD (Fluorescence Detector) after preparing analytical samples according to the preprocessing method of food revolution, The analytical conditions are as shown in the HPLC analysis conditions in Table 3 below, and the content formula was determined by the peak area method using the internal standard material according to the method of food revolution.

≪ Example 1 > (Primary extraction step)

A. Inert gas Preparation of ginseng berry primary extract concentrates according to the change chungjinryang

  Six extractors were prepared to correspond to the <Comparison Test> and <Test 1> to <Test 5> for the test of the ingredient content of the extract concentrate according to the change of the nitrogen filling amount. One kilogram of the dried product (purchased from Geumsan ginseng market) was added together with 2 liters of purified water, and nitrogen gas as an inert gas was supplied from the inner bottom of the extractor through the bubbling tube at an extraction temperature of 70 ° C at a flow rate of 0 ml / min, 2 ml / min, 6 ml / min, 8 ml / min, and 10 ml / min for 30 minutes and 30 minutes, respectively. The primary extraction and the first extraction were carried out for 8 hours, After the separation, each of the primary extracts was put into a falling film evaporator, and a solid content of 35 wt% was obtained under conditions of a concentration of 150 ° C, a vacuum pressure of 5 mmHg, a flow rate of the concentrate production flow rate of 50 ml / First Extraction of Concentrated Berries Of the title compound, respectively.

&Lt; Test Example 1 >

The concentration of the ginsenoside Re of the first extract solution of the jujube berry obtained in the <Comparative Test>, <Test 1> to <Test 5> And Rd, and the results are shown in [Table 4] and [Figure 3] below. The yield of the juice extract concentrate when the solid content was 35% by weight with respect to the amount of raw juice berry was calculated as weight%.

[Figure 3]

As shown in [Table 4] and [Figure 3], when the extraction condition is compared with the case where the nitrogen is not filled in the extraction process and the nitrogen is filled, there is no specific change in the process yield, The content of ginsenosides Re and Rd obtained at the time of filling with nitrogen was found to be higher than that at the time when nitrogen was charged. Especially, when the nitrogen was filled at 6 ml / min, the ginsenoside Re content was 29.04 mg / g, Since the content of senoside Rd is 26.4 mg / g, it can be seen that ginsenosides Re and Rd are increased to about 7 mg / g and 2.5 mg / g, respectively, Injection (filling) of nitrogen gas (extraction) in extracting condition to obtain maximum amount of prevention of transformation (breakdown) of ginsenoside Re and Rd contained in juice berry, It can be seen that it has an adverse effect.

That is, in the extraction of the fresh juice berry which is easily transformed from the analysis results of the above-mentioned [Table 4] and [Fig. 3], the conversion (destruction) of the ginsenosides Re and Rd contained in the fresh juice berry is prevented to the utmost, It can be confirmed that the method of producing a concentrated juice extract extract according to the present invention for extracting nitrogen gas by injection of nitrogen gas can achieve an excellent effect.

B. Preparation of Concentrated Extract of Ginseng Berry as a Primary Extract according to the Changes in the Contents of the Spirulina

  In order to test for the change in the content of the alcohol, the alcohol content is divided into 0 wt% to 90 wt% while changing the content of the alcohol as the extraction solvent in 10 wt% units, , A concentrate of jujube berry primary extract was prepared in the same manner as in <Test 3> of <Example 1>.

<Nos. 6> to <Test 13> were experimented by applying <Test 3> of <Example 1>, which is the condition with the best efficiency of 6 ml / min, The amount used was calculated as weight%.

&Lt; Test Example 2 &

The concentration of ginsenoside Re of the jujube berry primary extract concentrate obtained in <Test 6> to <Test 13> And Rd, and the results are shown in [Table 5] and [Figure 4] below.

[Figure 4]

As shown in [Table 5] and [Fig. 4], the contents of ginsenosides Re and Rd were significantly increased as the content of the extract solvent as the extraction solvent increased under the same conditions of the nitrogen filling 6 ml / min as the extraction conditions The content of ginsenoside Re was 29.24 mg / g at 0 wt% using pure water as an extraction solvent, whereas the content of ginsenoside Re was 35.93 mg / g when the extraction solvent was 70 wt% g, and the concentration of ginsenoside Rd was increased from 26.30 mg / g to 30.34 mg / g by 4 mg / g, To 60% by weight, the content of ginsenosides Re and Rd is not greatly changed, and the process yield is also reduced.

In the extraction of juvenile berry which is easily transformed from the analysis results in the above-mentioned [Table 5] and [Figure 4], the conversion (destruction) of the ginsenosides Re and Rd contained in the fresh juice berry is prevented as much as possible while obtaining a high content It can be confirmed that almost all of the intrinsic Re and Rd of the ginsenosides possessed by the juvenile berry can be extracted by the extraction according to the proper combination of the nitrogen content and the alcohol content as the extraction solvent. It can be predicted that the conversion (breakdown) of the ginsenosides Re and Rd contained in the juice berry can be prevented as much as possible while obtaining a high content of the ginsenosides contained in the juice berry under the extraction conditions by proper combination of the content of the alcohol as an extraction solvent.

&Lt; Example 2 > (Secondary extraction step)

C. Preparation of Concentrated Secondary Extract of Ginseng Berry according to Amount of Alpha-Amylase

  The procedure of <Test 11> of <Example 1> with the best extraction efficiency was carried out except that 8 kg of dried product (purchased from Geumsan ginseng market) with seeds suitable for the food standard was added to the extractor together with 16 liters of purified water And the first extraction concentrate and the first extraction residue were recovered, respectively.

The recovered primary extraction residue was divided into the same amount as that of the extracting unit 8 corresponding to the following <test a> to <test h> prepared for testing for the change in the amount of alpha-amylase added, Amylase was added to the extractor in an amount of 0.2% by weight and the mixture was divided into 0% by weight and 1.4% by weight. The mixture was then extracted at 80 ° C for 8 hours and separated into extracts and extracts Then, the extract was put into a falling film evaporator, and a juice extract secondary extract having a solid content of 35% by weight was prepared at a concentration of 150 ° C., a vacuum pressure of 5 mmHg, a flow rate of the concentrate production flow rate of 50 mL / min and a process time of 30 minutes .

&Lt; Test Example 3 >

The concentration of ginsenoside Re of the jujube berry second extract concentrate obtained in <Tests a> to <Test h> And Rd, and the results are shown in [Table 6] and [Figure 5] below.

[Figure 5]

As shown in [Table 6] and [Figure 5], when the amount of alpha-amylase added is increased from 0 wt% to 1.4 wt%, it can be seen that the process yield is more than doubled. More specifically, The process yield was increased to 8 wt% when 1 wt% of alpha-amylase was added. However, it was confirmed that the yield of 11 kinds including ginsenoside Re and Rd Showed almost no ginsenoside content.

According to the results shown in [Table 6] and [Figure 5], when the secondary extraction is carried out using alpha-amylase as a method for improving the process yield in the method of producing the jujube berry extract concentrate according to the present invention It can be confirmed that the process yield can be improved 2.3 times up to 8 wt%.

D. Preparation of Concentrated Secondary Extract of Ginseng Berry by Ultrasonic Injection

    In order to test for ultrasonic injection, the first extraction concentrate and the first extraction residue were obtained in the same manner as in <Example 2>, and the first extraction residue obtained was subjected to the following <Test> to <Test> And the extract was subjected to extraction in the same manner as in <Test f> of <Example 2> while changing the ultrasonic scanning time from 1 hour to 8 hours to prepare a jujube berry second extract concentrate.

<Test Example 4>

 The test yields of the jujube berry secondary extracts obtained in the above <Test> and <Test> were tested and analyzed. The results are shown in Table 7 below.

As shown in Table 7, when the ultrasonic scanning time was increased from 1 hour to 8 hours, when the ultrasonic scanning time was 3 hours, the process yield was maximum to 8% by weight, and the scanning time was longer than 3 hours , The process yield was not increased any more. According to the results shown in the above Table 7, in the process for producing the jujube berry secondary extract according to the present invention, %, It can be confirmed that the processing time can be shortened by 5 hours when the ultrasonic wave is injected.

&Lt; Example 3 >

In order to compare the preparation method of the final extract of concentrated juice of the present invention with the conventional method of preparing the concentrated juice extract of the present invention, each of the following extract concentrates was prepared. The present invention is characterized in that [Example 1] and [Example 2 ] Using the most efficient process conditions, yielding the final concentrate as follows: &lt; tb &gt; &lt; TABLE &gt;

1) Preparation of a final extract concentrate of fresh juice according to the present invention

  After extracting 2 liters of 70% by weight of the alcohol and 1 kg of the dried product of the fresh juice into the extractor, the extraction was carried out at an extraction temperature of 70 캜 for 8 hours and a nitrogen gas filling rate of 6 mL / min as an inert gas. Was charged into a falling film evaporator and a concentrated concentrate of jujuba berry having a solid content of 35% by weight was obtained under conditions of a concentration of 150 캜, a vacuum pressure of 5 mmHg, a flow rate of the concentrate production flow rate of 50 mL / min and a process time of 30 minutes ,

After extracting 2 L of purified water from the above-mentioned primary extraction residue as a raw material and extracting it under the conditions of extraction temperature of 80 캜, 1% by weight of alpha-amylase addition amount and 3 hours of ultrasonic injection, And the extract solution was put into a falling film evaporator. The extract solution was passed through a Falling Film evaporator and subjected to filtration at a concentration of 150 캜, a vacuum pressure of 5 mmHg, a flow rate of the concentrate production flow rate of 50 mL / min and a process time of 30 minutes. , And then the obtained primary extract concentrate was mixed to obtain a final extract concentrate.

2) Manufacture of concentrated extract of jujube berry by conventional methods

   As a conventional process condition for extracting jujube berry extract, 2 liters of 70 wt% of alcohol and 1 kg of dried berry were added to the extractor, and the extract was extracted at an extraction temperature of 70 ° C for 8 hours. Was placed in a batch circulating vacuum concentrator, and a concentrate of jujube berry primary extract having a solid content of 35% by weight was obtained under the conditions of a concentration temperature of 220 ° C and a vacuum pressure of 5 mmHg. The extracted extract residue was used as a raw material, The extract was again extracted under the same conditions as above, and the extract was concentrated to obtain a juice secondary extract concentrate having a solid content of 35% by weight, and then the juice first and second extract concentrates obtained above were mixed to obtain a final product .

&Lt; Test Example 5 >

The contents of ginsenosides Re and Rd and the process yields of each final extract concentrate obtained by the method of the present invention and the conventional method in the above Example 3 are shown in Table 8 below.

As shown in Table 8, the production method of the jujube berry extract concentrate according to the present invention was 26.7%, 28.8%, and 10.9%, respectively, compared to the conventional process for obtaining the juice extract concentrate .

Finally, along with the excellent results of the present invention shown in Examples 1 to 3, there is a limit to the yield of fresh berry which can be collected only over 4 years, In particular, when considering the industrial value of ginsenosides Re and Rd, which are unique physiologically active ingredients contained in fresh juice berry, the yield and yield of ginsenosides Re and Rd, which are the main physiologically active ingredients of jujube berry, can be greatly improved It can be predicted that the present invention is a very advantageous technique.

Claims (8)

a) Extracting raw material Into the raw material mixture containing the berry and the alcohol containing the alcohol extract, the inert gas is injected in the range of 2 ml / min to 10 ml / min per Kg of the fresh berry and the first extract and the first extraction residue A first extraction step of separating the first extraction liquid and then concentrating the first extraction liquid to recover the first extraction concentrate,
b) Starch hydrolyzate, alpha-amylase, was added to the primary extracts separated in the first extraction step, purified water was added, and secondary extraction was performed with ultrasonic waves for 1 to 8 hours to separate into secondary extract and secondary extraction residue A second extraction step of concentrating the second extraction liquid to recover the second extraction concentrate,
and c) a final extraction concentrate recovery step of obtaining a final extraction concentrate by mixing the primary and secondary extraction concentrates recovered in the primary and secondary extraction stages. delete  The method according to claim 1, wherein the inert gas is argon gas or nitrogen gas. [Claim 4] The method according to claim 3, wherein the amount of the extraction solvent used in the first extraction step is twice the weight of the raw material berry. [Claim 5] The method according to claim 4, wherein the alcohol content of the alcohol-containing extraction solvent ranges from 10% by weight to 90% by weight based on the weight of the purified water. [Claim 6] The method according to claim 5, wherein the amount of alpha-amylase added in the second extraction step ranges from 0.2 wt% to 1.4 wt% with respect to the weight of the raw material berry. delete The method according to any one of claims 1 to 7, wherein the concentration of the primary and secondary extracts is concentrated by falling film type concentration at a concentration of from 1O &lt; 0 &gt; C to 160 & Wherein the concentrate is a concentrate of the extract.

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