KR101429937B1 - Cosmetic compositon using herbal rice wine - Google Patents

Abstract

The present invention relates to a cosmetic composition for antioxidant and whitening comprising an oriental herb extract.

Description

{COSMETIC COMPOSITON USING HERBAL RICE WINE}

The present invention relates to a cosmetic composition using an oriental herb.

In recent years, cosmetics are preferred not only to supply oil and moisture to the skin but also to whitening, antioxidant and anti-inflammatory functions. In addition, cosmetics using active ingredients derived from natural products are popular, and functional cosmetics derived from natural products have been developed.

On the other hand, makgeolli is traditional Korean takju, and consumption has increased recently, and exports to Japan and other countries are also increasing. Accordingly, there is an effort to improve the manufacturing process of makgeolli or improve the characteristics of makgeolli by adding specific additives. Korean Patent No. 1092482, for example, discloses a fruit makgeolli added with a fruit fermentation liquid to a traditional rice rice wine, and Korean Patent No. 1037132 discloses a functional takju prepared using agar bean. However, most of these researches are about makgeolli as food, and the use of makgeolli as a raw material of functional cosmetic has not yet been studied.

Accordingly, the inventors of the present invention have conducted research to prepare functional cosmetics by improving the makgeolli, and have found that rice koji prepared by using rice instead of general makgeolli, that is, rice gruel, , The present inventors have found that the whitening and antioxidative effect of the herbal extract of Oriental herbarium is excellent and completed the present invention.

It is an object of the present invention to provide a cosmetic composition for antioxidant and whitening.

In order to achieve the above object, the present invention provides an antioxidant and whitening cosmetic composition comprising a herbal extract of Oriental herbarium comprising an extract of Gugija, Saururus thunbergii, .

According to another aspect of the present invention,

Mixing the diced rice with a starch raw material to perform a single-stage dipping;

Preparing a one-packed bean jam by mixing the first-step beads with an extract of Gugija, Saururus chinensis, Saururus chinensis, Saururus chinensis, Licorice root,

And extracting the oriental herbal extract. The present invention also provides a method for producing an antioxidant and whitening cosmetic composition.

The cosmetic composition of the present invention has antioxidant, whitening, skin inflammation relieving ability and wrinkle improving ability.

Figs. 1 and 2 show tyrosinase inhibitory activities of the extracts of Oriental herbal extracts and Chinese herbal extracts.
FIGS. 3 and 4 show the melanin production inhibitory effect of the extracts of the Korean herbal extracts and the Korean herbal extracts.
FIG. 5 shows the inhibitory effect of TRP-1 and TRP-2 on the expression of Herbal Extract and Herbal Extract.
FIGS. 6 and 7 show the enzyme activity inhibitory activity of the herbal extracts of Chinese herbal extract and Chinese herbal extract.
FIG. 8 shows the inhibitory activity of MMP-2 and MMP-9 on the enzyme activity of the extracts of Oriental herbal extracts and Miltaria officinalis, and FIG. 9 shows the inhibitory effect on the expression of MMP-2 and MMP-9.
Figs. 10 and 11 show the results of the cytotoxicity evaluation of the extracts of Oriental herbal extracts and the extracts of Oriental herbivorous plants.
Figures 12 and 13 show the ability of the extracts of Oriental herbarium extract and R. indigotorum extract to inhibit skin inflammation.
Figs. 14 and 15 show the ABTS radical scavenging ability of the extracts of Oriental and Oriental hyphae.

The present invention relates to an antioxidant and whitening cosmetic composition comprising an extract of Oriental herbarium, and a cosmetic composition for antioxidative and whitening characterized in that the oriental herb extract contains extracts of gugija, saururus, saururus, saururus and licorice.

According to another aspect of the present invention,

Mixing the diced rice with a starch raw material to perform a single-stage dipping;

Preparing a one-packed bean jam by mixing the first-step beads with an extract of Gugija, Saururus chinensis, Saururus chinensis, Saururus chinensis, Licorice root,

And extracting the oriental herbal extract, and a process for producing a cosmetic composition for antioxidant and whitening.

BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention, and the manner of achieving them, will be apparent from and elucidated with reference to the embodiments described hereinafter in conjunction with the accompanying drawings. It should be understood, however, that the invention is not limited to the disclosed embodiments, but is capable of many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, To fully disclose the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims.

Hereinafter, the present invention will be described in detail.

Hanbang Lee Hwa-ju

The herbal extract of Oriental herbaceous plant of the present invention is a herbal extract prepared by adding herbal extract. Ehwajo is a type of Takju produced by using Ewha songs, and rice hwa is a traditional yeast made of rice.

Ewha Song

Ryeongguk is a rice koji which is made by crushing rice called water and cultivating it in a specific shape. Its shape is spherical, about 8 ~ 10cm in diameter. The production of Rika song consists of preparation of raw materials, bending (molding), cultivation, legalization and commercialization process.

Pine needles (pine needles - well-dried cut pine leaves) are molded into rice cakes and placed in boxes, and then sprayed on the surface of rice cakes to play a role of providing good ventilation during cultivation of rice cakes. 5 to 10% by weight.

The molding of the dancing piece is performed by the following process. First, rice is sifted 5 to 10 times, and rice is immersed in cold water at 10 ° C or less for at least 3 hours and at least 1.5 times of washed rice. Then, for 3 to 4 hours, drain the rice while stirring the rice at intervals of 30 minutes. Then, rice is finely pulverized to such a degree that it can pass through a 20 mesh sieve, and rice powder is selected. The selected rice powder is used to form a dancing piece. At this time, the dancing piece is rounded to a size of about 8 to 10 cm. The weight per piece of the dough is preferably 200 to 250 g. Spread 40 ~ 50g of dried pine needles on a paper box and put a piece of rice cake on it. Spread the pine needles over it and transfer it to the incubator to start culturing. Cultivation of Ehwa is performed in a temperature and humidity chamber maintained at a temperature of 25 ~ 35 ℃ and a humidity of 40 ~ 60%, and the total cultivation time is 7 ~ 10 days.

After the cultivation is complete, the hyphae are cleaned by removing the hyphae and spores on the surface of the rice bran and dividing the rice bran into twelve equal parts to check the contamination of the rice bran. Then, in the place where the wind blows weakly and is not damp and sunny, it progresses the drying and ripening of the song for about one week.

The process of commercializing Ewha Song is as follows. Fine dried rice bran is finely pulverized by a dry grinder, and the pulverized rice bran is selected and analyzed for glucoamylase activity, microbial analysis, moisture content, etc. and prepared for the production of oriental herb.

Hanbang Lee Hwa-ju

The starch feedstock for the production of one-packed rice wine according to the present invention comprises rice cake, rice cake powder, uncooked rice, crushed rice after crushing with a crusher after crushing, crushed rice cake, It is crushed.

The oriental herbaceous plant of the present invention is manufactured through a one-stage soaking process and a two-stage soaking process, in which no additional entry or yeast is added.

The one-stage dipping is performed by mixing 100 parts by weight of the starch raw material with 90-120 parts by weight of rice cakes (rice koji) and 80-120 parts by weight of water, followed by fermentation at 20-25 ° C for 7-10 days. Yeast or other yeast other than Eichae are not added during the first stage of dipping.

The fermentation is carried out at 20 ~ 25 ℃ for 20 ~ 30 days after the starch raw materials are mixed with the rice cake (rice koji), water, herbal extract, At this time, the starch raw material of the two-stage immersion is 100-200 parts by weight, preferably 80-150 parts by weight, more preferably 100 parts by weight, of the starch raw material used in the one-stage immersion, The weight ratio of the raw material, the dicer and the water is the same as the weight ratio of the starch raw material, the dicer and the water used in the first stage soaking.

The herbal extract is an extract of the herbal medicine, which is prepared by mixing the same amount of gugija, licorice, mallow, and saururus chinensis by hot water extraction. Preferably, the herbal extract of the present invention is prepared by using 5 to 10% by weight of the herbal medicine ingredient of the starch ingredient used in the two-stage soaking, wherein the weight of the herbal medicine is 2 to 10 times, preferably 2 to 5 times And extracted with hot water for 1 to 6 hours, preferably 2 to 4 hours.

After separating the sake lees through the two-step soaking process, the one-way seaweed is completed. The fermentation process is the process of separating sake lees (starchy raw material) from fermented sake using a separator.

Oriental herb extract

The herbal extract of the present invention means that the herbal extract is concentrated, lyophilized or extracted with water or an organic solvent and fractionated. The organic solvent may be an alcohol having 6 or less carbon atoms, ethyl acetate, chloroform or the like, preferably ethanol or ethyl acetate.

Preferably, the herbal extract of the present invention may be a lyophilized lyophilized product obtained by removing the alcohol from the herbal extract and lyophilized. Preferably, the herbal extract of Oriental herbarium of the present invention may be an ethanol extract of Oriental herbarium obtained by extracting the herbal extract with ethanol and then lyophilized with the ethanol extract. Preferably, the herbal extract of the present invention may be a fraction obtained by fractionating the ethanol extract of the herbal extract with ethylacetate.

Including herbal extracts Cosmetics  Composition

The cosmetic composition comprising the herbal extract of the present invention has whitening, antioxidant, skin inflammation-relieving ability and wrinkle-improving ability. Specifically, the cosmetic composition comprising the extract of Oriental herbal extract of the present invention has a tyrosinase activity inhibiting ability, a melanin formation inhibiting ability and an active oxygen scavenging ability.

Cosmetics  Composition

The cosmetic composition of the present invention may be prepared in any formulations conventionally produced in the art and may be formulated into a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant- containing cleansing oil, powder foundation , An emulsion foundation, a wax foundation and a spray, but is not limited thereto. The cosmetic composition of the present invention may also be formulated as a softening agent, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

≪ Materials and methods >

In the present invention, commercially purchased rice (Gyeonggi) was used, and domestic products such as Gugija, Saururus chinensis, Sambucopia and Licorice were purchased from the market.

≪ Production of Rika song &

About 60 kg of rice (rice) and about 0.5 kg of boughs (well-dried cut pine leaves) were prepared. First, rice was semi- finished 8 times and then immersed in 90 kg cold water at 7 ~ 8 ℃ for 4 hours. After 30 minutes, the rice was removed and the water was removed for 3 hours. The rice was crushed and passed through a 20 mesh sieve, and the rice powder was selected. Then, the rice powder was rolled up to a size of 8 to 10 cm, and the rice cake was formed. The weight of each of the above-mentioned Eichung songs was 200 to 250 g, and 40 to 50 g of the boughs were scattered in a paper box, and then the Eichae songs were inserted. The bloom was scattered again on it and cultured for 9 days in a constant temperature and humidity (incubator) maintaining the temperature at 25 to 35 ° C and a humidity of 40 to 60%.

After that, the mycelium and spores on the surface of Ryeongbyeong were cleaned out, and the rice dumplings were split into twelve equal parts to check the contamination inside. And it was dried and aged for about a week in a place where the wind was weak and it was not damp and sunny. After that, the well - dried Eichae were finely pulverized with a dry grinder, and the pulverized Eichae was selected through glucoamylase titration, microbial analysis, and moisture content measurement.

≪ Preparation of oriental herb extract >

1 kg of Gugija, Saururus chinensis, Saururus chinensis, Lycopersicon esculentum and Licorice were prepared and extracted with 8.5 kg of water for 3 hours.

≪ Preparation of oriental orientalis '

30 kg of rice was semi-aged for 1-2 hours and pulverized using a grinder. 30 kg of the above ground rice flour was mixed with 32 kg of rice cakes (rice koji) and 33 kg of water, followed by fermentation at 20 to 25 ° C for 8 days to perform one stage of immersion. At this time, no yeast or another yeast other than the rice cake was added.

30 kg of rice was semi-prepared and ground for 1-2 hours and then pulverized using a pulverizer. 30 kg of the above ground raw rice was mixed with 32 kg of diced rice, 33 kg of water, 3.1 kg of the herbal extract, and the first stage fermented product, followed by fermentation at 20 to 25 ° C for 25 days to perform two stages of immersion.

After the two - stage immersion was completed, the sake lees were separated from the sake lees using a sorting machine to complete the one - sided lees.

≪ Production of Ehwaju &

Except that the herbal extract was not added during the two-stage immersion, the same procedure as in the above < preparation of oriental oriental hyaluronan &quot;

&Lt; Examples 1 to 3 >

The alcohol component was removed from the herbal extract using a vacuum concentrator and then lyophilized to prepare a lyophilized lyophilized product (Example 1).

95% ethanol was added to the herbal mixture so that the concentration of ethanol in the herbal mixture became 80%, followed by stirring at low temperature (10 ° C) for 24 hours. Then, 80% ethanol was collected, and 80% ethanol was re-added to the remaining one-packed rice husk solids, and the mixture was further stirred at low temperature for 24 hours for secondary extraction. Thus, an 80% ethanol extract of Oriental herbarium was obtained in the second step. The extract was concentrated under reduced pressure to remove ethanol, followed by lyophilization to prepare a lyophilized product of the extract of Oriental herbarium (Example 2).

A part of the lyophilized product of the extract of the oriental herbal extract was fractionated with ethyl acetate and lyophilized (Example 3).

&Lt; Comparative Examples 1 to 3 >

The alcohol component was removed from the above-mentioned aliquot using a vacuum concentrator and then lyophilized to obtain a lyophilized product (Comparative Example 1).

95% ethanol was added to the above-mentioned aliquots to adjust the ethanol concentration in the aliquots to 80%, followed by stirring at low temperature (10 ° C) for 24 hours. Then, 80% ethanol was collected, and 80% ethanol was re-added to the remaining solid of Hwangju and further stirred at low temperature for 24 hours for secondary extraction. Thus, an 80% ethanol extract of Echinacea was obtained in a second step. The extract was concentrated under reduced pressure to remove ethanol, followed by lyophilization to prepare a lyophilized product of Ehwangju ethanol extract (Comparative Example 2).

A portion of the lyophilized product of the Ehwangju ethanol extract was fractionated with ethyl acetate and lyophilized (Comparative Example 3).

<Experimental Example 1> Skin whitening activity evaluation

In bito Tyrosinase  Evaluation of inhibitory activity

To the 96-well plate, 210 μl of 0.1 M phosphate buffer (pH 6.5) was added, and 40 μl of L-tyrosine, 20 μl of tyrosinase and 30 μl of the sample were added thereto and reacted at 37 ° C. for 10 minutes. The absorbance was measured. For comparison, Kojic acid was used for the comparison. The inhibitory activity was calculated according to the following formula 1.

<Formula 1>

Inhibition activity (%) = {1- (sample OD-sample blank) / control OD} 100

Melanin production Inhibition  evaluation

Cells (melanocyte cells; B16F1) were plated in DMEM containing 10% FBS at 5 × 10 ⁵ cells / ml (final volume 3 ml) in a 60 cm plate and incubated at 37 ° C in a 10% CO ₂ incubator (MCO-20AIC, Sanyo) Time. After the culture, the medium was removed and the diluted samples were added to the DMEM medium containing 40 ng / ml of α-MSH for 3 days. On day 3, 200 [mu] l of the medium cultured on a 60 [phi] plate was divided into 96 well-plates and absorbance was measured at 490 nm. Cells from which the medium was removed were treated with 0.25% trypsin-EDTA (Sigma Chemical Co.) solution to recover the cell pellet, transferred to a 1.5 ml tube and centrifuged at 10,000 rpm for 10 minutes to remove the supernatant. The resulting pellet was dried, and 200 μl of 1N NaOH was added thereto to dissolve the melanin in the cells at 80 ° C for 30 minutes. The absorbance of this solution was measured at 405 nm in an ELISA reader to determine the melanin content of the sample. Cells without addition of the sample were used as a control, and compared with the melanin content in the control, the degree of melanin production of the test material was measured to determine the whitening effect.

TRP -1 and TRP -2 expression analysis

Cells were cultured in 60 × cell culture plates at 1 × 10 ⁶ cells / ml for 24 hours, washed twice with PBS, and then diluted to a concentration of 1 × 10 ⁶ cells / ml. Cells were treated with protein concentration buffer Lt; / RTI &gt; cells. The Western blot method was used to measure the inhibitory activity of TRP (tyrosine related protein) -1 or TRP-2, and the amount of total protein in the lysed cells was quantitated using the Bradford method. Proteins from lysis were electrophoresed on 10% SDS-PAGE and transferred to PVDF gel proteins. The primary antibodies of TRP-1 or TRP-2, respectively, were diluted in a blocking solution at a ratio of 1: 1000 and reacted at 4 ° C for 12 hours. After the primary antibody reaction, the cells were washed with 0.1% TBST (Tris-buffer Saline Tween 20) three times for 10 minutes each. The secondary antibody recognizing TRP-1 or TRP-2 was diluted to 1: 1000 in 5% non-fat milk milk, reacted at room temperature for 2 hours, washed with 0.1% TBST for 10 minutes in the same manner as the primary antibody And then developed by chemiluminescence.

result

In bito Tyrosinase  Evaluation of inhibitory activity

Example 1 inhibited the activity of tyrosinase in a dose-dependent manner, whereas the inhibitory effect was insufficient in Comparative Example 1 (Fig. 1). In addition, Example 2 and Example 3 also inhibited tyrosinase activity in a dose-dependent manner, and the inhibitory activity was equal to or higher than that of kojic acid. However, Comparative Examples 2 and 3 did not have such an effect (Fig. 2).

Melanin ( Melanin ) Production inhibition efficacy evaluation

In the case of Example 1, melanogenesis inhibitory activity was confirmed to be about 15% at a concentration of 100 ug / ml, and arbutin used as a positive control showed about 20% inhibitory activity at a concentration of 100 ug / ml. On the other hand, Comparative Example 1 had no inhibitory effect on melanin synthesis (Fig. 3).

On the other hand, Example 2 inhibited melanogenesis in a concentration-dependent manner, and showed an inhibitory activity equal to or higher than that of the positive control group arbutin at a concentration of 50 ug / ml or higher (Fig. 4).

TRP -1 and TRP -2 expression analysis

Analysis of TRP-1 and TRP-2 using Western blot revealed that Test Example 2 and Test Example 3 inhibited TRP-1 and TRP-2 in a concentration-dependent manner, whereas Comparative Example 2 and Comparative Example 3 But did not inhibit TRP-1 and TRP-2 (Fig. 5). Therefore, it was judged that the herbicide inhibited the biosynthesis of melanin by inhibiting TRP-1 and TRP-2.

<Experimental Example 2> Evaluation of skin wrinkle improving ability

Elastase  Evaluation of inhibitory activity

0.5 mL of each of 50 mM Tris-HCl (pH 8.0) and elastase (10 g / mL in 50 mM Tris-HCl, pH 8.0) was added to each well and 0.1 mL of a certain concentration of the sample solution was added thereto. &Lt; / RTI &gt; for 5 minutes. 1.0 mL of a substrate solution (0.8 mM N-succinyl- (L-Ala) 3-p-nitroanilide, in 50 mM Tris-HCl buffer (pH 8.0)) was added and reacted at 37 ° C for 10 minutes. Change was measured.

Matrix metalloproteinase ( MMP ) -2 / -9 &lt; / RTI &gt; Zymography )

Cells were cultured for 24 h at 60 × cell culture plate at 1 × 10 ⁶ cells / ml. After the cells were washed twice with PBS, 1 ml of PBS was added to each well. The prepared cells were irradiated with UVB (312 nm) at 20 mJ / cm ² . After 3 days from UV irradiation, take the medium, transfer to 50 ml Centricon, and centrifuge at 3,000 rpm for 30 minutes. The amount of protein was quantitated by Bradford method and electrophoresed on 10% SDS-PAGE, washed twice with 0.1% triton-X for 30 minutes, and then incubated at 30 ° C for 18 hours in developing buffer. And stained with blue.

Matrix metalloproteinase ( MMP ) -2 / -9 expression Low performance

Cells (keratinocyte cells; HacaT) are cultured on a 60 φ cell culture plate at 1 × 10 ⁶ cells / ml for 24 hours, washed twice with PBS, and then the PBS is dispensed in 1 ml increments. The prepared cells are irradiated with UVB (312 nm) at 20 mJ / cm ² . After UV irradiation, the PBS was removed, and the diluted samples were treated with the cells for 3 days, and the cells were lysed with protein concentration buffer. The Western blot method was used to measure the inhibitory activity of MMP-2 or MMP-9, and the amount of total protein in the lysed cells was quantified using the Bradford method. Proteins from lysis were electrophoresed on 10% SDS-PAGE and transferred to PVDF gel proteins. The primary antibodies of MMP-2 or MMP-9, respectively, were diluted in a blocking solution at a ratio of 1: 1000 and reacted at 4 ° C for 12 hours. After the primary antibody reaction, wash with 0.1% TBST (Tris-buffer Saline Tween 20) three times for 10 minutes each. The secondary antibody recognizing MMP-2 or MMP-9 was diluted to 1: 1000 in 5% skim milk and reacted at room temperature for 2 hours. The antibody was washed with 0.1% TBST for 10 minutes in the same manner as the primary antibody And then developed by chemiluminescence.

result

Elastase  Evaluation of inhibitory activity

The lyophilizates of Example 1 and Comparative Example 1 inhibited the enzymatic activity of elastase in a concentration-dependent manner, and the inhibitory activities between the two experimental groups were confirmed to be similar (FIG. 6). In addition, Example 2 and Comparative Example 2 also inhibited the enzymatic activity of elastase in a concentration-dependent manner (Fig. 7).

MMP -2 and MMP -9 Activity Inhibition Assessment

Example 2 and Example 3 inhibited the enzyme activity of MMP-2 and MMP-9 in a concentration-dependent manner (Fig. 8), and inhibited intracellular protein expression of these proteins (Fig. This effect of the oriental oriental herb has been observed to be similar to the effects of Comparative Example 2 and Comparative Example 3.

<Experimental Example 3> Evaluation of skin sedation and anti-inflammatory activity

Cytotoxicity Assessment

In order to confirm the cell survival rate to evaluate the wrinkle-improving effect of the skin, MTT assay [3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide reduction using RAW264.7 cell, Murine macrophage cell line method]. First, RAW 264.7 cells were seeded at 2 × 10 ⁵ cells per well of a 96-well plate and cultured in DMEM medium containing 10% FBS at 37 ° C. under 5% CO ₂ for 24 hours. After the primary culturing, Example 2 and Example 3 were treated for each concentration, and the medium was replaced with a medium to which no FBS was added, followed by secondary culturing for 24 hours. Then, 100 μl of MTT solution was added to the medium, left for 4 hours, and then the medium was removed. 100 μl of DMSO solution was added to each well, stirred for 20 minutes, and then absorbance was measured at 540 nm using a microplate reader.

LPS Evaluation of Inhibitory Activity on Skin Inflammation

Murine macrophage cell line RAW264.7 cells were adjusted to a concentration of 1 x 106 cells / ml using RPMI 1640 medium containing penicillin (100 IU / ml), streptomycin (100 μg / ml) and 5% FBS , And then inoculated in a 24-well plate and pre-cultured for 18 hours under the conditions of 5% CO 2 and 37 ° C. Then, the medium was removed and 50 ㎕ of the test substance prepared at a 10-fold concentration and 450 쨉 L of a medium containing LPS (final concentration 1 g g / ml) were simultaneously treated and cultured. After 24 hours, the culture medium was centrifuged (12,000 rpm, 3 minutes), and a portion of the supernatant was stored at -20 ° C or lower before quantification. NO determination was carried out using Griess solution (0.5% naphthylethylenediamine dihydrochloride, 5% sulfanilamide, 25% H3PO4) and the calibration curve was prepared using sodium nitrite as a standard.

result

Cytotoxicity Assessment

Example 1 and Comparative Example 1 did not induce cytotoxicity in the macrophage cell line RAW264.7 (FIG. 10). In addition, cytotoxicity of Examples 2 and 3 and Comparative Examples 2 and 3 was evaluated by MTT assay, which did not cause toxicity to RAW264.7 cells (FIG. 11A). Particularly, in Example 3 having excellent anti-inflammatory activity, cell viability of 80% or more was confirmed (FIG. 11B).

LPS Evaluation of Inhibitory Activity on Skin Inflammation

Example 1 inhibited the production of nitric oxide (NO), a kind of inflammatory reaction induced by LPS in a concentration dependent manner, and inhibited NO production about 50% at a concentration of 100 ug / ml. This confirms that the Example 1 was not toxic to RAW264.7 cells, and thus, it is judged that the effect of the ingredient in Example 1 is effective. However, in Comparative Example 1, no inflammatory reaction inhibitory activity was confirmed (FIG. 12).

In addition, with respect to the LPS-induced inflammatory reaction inhibition experiment, Example 2 and Example 3 were confirmed to inhibit NO production by 50% or more (Fig. 13A). In particular, Example 3 inhibited the production of NO in a concentration-dependent manner, and inhibited NO production by 70% or more without cytotoxicity at a concentration of 50 ug / ml or more (Fig. 13B). However, Comparative Example 2 and Comparative Example 3 failed to confirm the effect of inhibiting inflammation.

<Experimental Example 4> Evaluation of antioxidative activity

ABTS Radical Scatters

ABTS and 1.0 mM AAPH were mixed with 100 mM phosphate buffer (pH 7.4) supplemented with 150 mM NaCl, heated in a 68 ° C water bath for 30 minutes, and cooled at room temperature for 10 minutes. The ABTS solution was diluted with buffer to give an absorbance value of 0.7 ± 0.02 at 734 nm. 990 μl of ABTS solution with absorbance value was added to 10 μl of sample solution, incubated at 37 ° C for 10 minutes, and absorbance was measured at 734 nm.

result

ABTS Radical Scatters

Example 1 showed about 10% higher activity oxygen scavenging ability than Comparative Example 1 (FIG. 14). In addition, both of Hanwha Ewha and Hwajae showed excellent active oxygen scavenging ability in ethyl acetate fraction rather than ethanol extract (Fig. 15).

Claims (10)

A cosmetic composition for whitening comprising an oriental herb extract,
The cosmetic composition for antioxidant and whitening according to claim 1, wherein the herbal extract contains hot water extract of Gugija, Saururus chinensis, Saururus chinensis, Saururus chinensis and Licorice.
The method according to claim 1,
Characterized in that said oriental herbaceous plant is produced by using an Eichae song.
3. The method of claim 2,
The cosmetic composition according to claim 1, wherein the rice cake is rice koji prepared by pulverizing rice called water to obtain a specific shape and culturing it.
The method according to claim 1,
Wherein the oriental herb is produced without admission or yeast.
The method according to claim 1,
The cosmetic composition according to claim 1, wherein the herbal extract is a fraction obtained by extracting the herbal extract with ethanol, lyophilizing the ethanol extract, and fractionating the lyophilized product with ethyl acetate.
The method according to claim 1,
Wherein the cosmetic composition has skin inflammation-relieving ability.
The method according to claim 1,
Wherein the cosmetic composition has a tyrosinase activity inhibiting activity and a melanin formation inhibiting activity.
The method according to claim 1,
Wherein the cosmetic composition has an active oxygen scavenging ability.
Molding rice cake using rice;
Mixing the diced rice with a starch raw material to perform a single-stage dipping;
Preparing a one-packed bean jam by mixing the first-step bean curd with the hot-water extract of Gugija, Saururus saurus, Saururus saururis, Saururus saururis and Licorice,
And extracting the oriental bean curd refuse.
10. The method of claim 9,
Characterized in that no entry is made or no yeast is added during the production of the oriental herb.

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