KR101383243B1 - Cultivation Method for Spinach containing Selenium - Google Patents

Abstract

The present invention comprises the steps of (a) sowing spinach seeds; And (b) cultivating spinach while fertilizing fertilizer containing selenium, thereby providing a new method for cultivating functional spinach containing high concentrations of selenium. The present invention can be used to enable the absorption of organic selenium through the plant by utilizing a special fertilizer, and also to combine the special mineral components with organic methods to enable the production of functional and eco-friendly agricultural products.

Description

Cultivation Method for Spinach containing Selenium

The present invention relates to a method of cultivating spinach containing selenium.

Selenium is a trace element distributed mainly in the liver, kidneys, heart and spleen and is a component of glutathione peroxidase (GPX1), a powerful antioxidant enzyme that removes harmful oxygen from the body. Selenium is absorbed from the soil by plants in the form of inorganic compounds of selenate or selenite, which are the constituents of glutathione peroxidase, selenomethionine and selenomethionine ( conversion to organic compounds such as selenosysthionine has antioxidant activity (Ellis Danielle R., et al., Current Opinion in Plant Biology pp. 273-279 (2003). In addition, antioxidation and cancer prevention (Finley, JW, J. Agric) are antioxidants that inhibit or eliminate the production of organic peroxides and hydrogen peroxide in the body together with Superoxide dismutase (SOD) or catalase. . Food Chem . 46: 3702-3707 (1998); Ip C., J. Nutr . 128: 1845-1854 (1998); Lu J. et al., Carcinogenesis 17: 1903-1907 (1996)). Iodothyronine deiodinase, a selenium-dependent enzyme, transforms the inactive thyroid hormone T4 secreted from the thyroid gland into the active T3, and selenium directly affects thyroid hormone. In addition, selenium protein (GPX4) is a component of the spermatozoa mitochondrial outer membrane of testes, and selenium is involved in sperm maturation, motility and production of androgen. Detoxification of toxic heavy metals such as arsenic, lead and cadmium (Hu Qiuhui, et al., Rural Ecoenviron . 16: 54-57 (2000).

Severe cases of selenium deficiency Acute or common heart disease and kesan disease (Ge K, et al., Am . J. Clim . Nuri . 57: 259-263 (1993)), mainly in children and women of childbearing age There is an increased risk of cancer, heart disease and reduced immune function, and it can lead to other red blood cell depression, myalgia, myocardial degeneration, muscle tension enhancement and pancreatic degeneration.

Selenium is made from nutritious yeast, seawater, algae, garlic, mushrooms, tuna, bread, ham, eggs, flour, beef, chicken, agricultural crops without fertilizers, seafood, milk, eggs, whole grains and almost all It is contained in vegetables, but vegetables and fruits are low in content. The recommended daily dose of selenium, published by the United Nations Food and Agriculture Organization and the World Health Organization, is 50-200 μg for adults. The recommended intake for cancer prevention is higher than 500 μg, which is higher than this. Selenium dosages of up to 1000-2000 μg. The daily intake of Korean people is known as 43 ㎍, which is less than the minimum recommended amount. In addition to the intake of selenium, depending on the chemical form of selenium, the bioavailability of selenium, metabolic processes in the body, biochemical function and toxicity are affected. In natural products, selenium is present mainly in the form of selenomethionine and selenocysteine, and organic selenium has a higher body absorption rate than inorganic selenium. Plants biosynthesize seleniummethionine using selenium absorbed from the soil and store it in proteins. On the other hand, animals cannot biosynthesize methionine, so the selenomethionine introduced into the body through the plant diet is converted from the liver into selenocysteine, and the physiologically active selenium protein is used for synthesis or excreted in vitro. The selenium content in the plant increases in proportion to the selenium content of the soil. Selenomethionine, which is ingested through plant foods, is stored in the body protein as the constituent amino acid of the protein in the body.

The selenium content of each food depends on the geographical origin of the raw crop growing soil (M. Eurola. Et al., Cereal Chem . 67: 334-337 (1990), Gupta, UC, et al., Plant Anal . 31: 1791-1807 (2000)), grains grown in Korean soil known as selenium deficiency area have low selenium content. Finland, which lacks selenium in the soil, has legislated the addition of selenium to fertilizers since 1984 to increase selenium (Reilly, C. Trends in Food Sci . & Tech . 9: 114-118 (1998).

In addition, selenium is susceptible to water and heat, and even if it is consumed as food, it is easy to lose before metabolism. However, organic selenium containing plants has the advantage of having high absorption rate, low toxicity, and easy accumulation in the body (Xu, HB). , Press of engineering college of central China (1983).

Generally, the selenium content in the soil is 0.1-2 mg / kg and consists of inorganic and organic compounds, and the mobility of selenium can be reduced by plant roots under crop cultivation conditions that improve selenium adsorption in the near-sphere (Plant and Soil 278: 298-301 (2005). Although selenium is used for producing functional agricultural products in rice, red pepper, soybeans, green beans, garlic, tomatoes, and mushrooms, the case of applying the selenium farming technique to spinach, a green vegetable containing a large amount of antioxidants (lutein and carotenoid), There is no situation.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

The present inventors have tried to develop a method capable of efficiently cultivating spinach containing selenium. As a result, sowing the spinach seeds; By developing a novel spinach cultivation process comprising cultivating spinach while foliar fertilizer containing selenium, according to this process, by confirming that it is possible to provide a functional spinach containing high concentration of selenium, To complete.

Accordingly, it is an object of the present invention to provide a method for cultivating spinach containing selenium.

Another object of the present invention is to provide a spinach containing selenium.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention and claims.

According to one aspect of the invention, the invention provides a method of cultivating spinach containing selenium comprising the following steps:

(a) sowing spinach seeds; And

(b) growing spinach while foliar fertilizer containing selenium.

The present inventors have tried to develop a method that can efficiently develop spinach containing selenium. As a result, we have developed a novel spinach cultivation process that includes cultivating spinach while foliar fertilizing fertilizer containing selenium, and according to this process, it was confirmed that it is possible to provide a functional spinach containing high concentration of selenium. .

According to a preferred embodiment of the present invention, the selenium is K ₂ SeO ₃ , K ₂ SeO ₄ , Na ₂ SeO ₃ , Na ₂ SeO ₄ , CaSeO ₃ , CaSeO ₄ , SeO ₃ , H ₈ N ₂ O ₄ Se and H ₈ N ₂ O ₃ Se, and at least one selenium selected from the group consisting of these.

According to a preferred embodiment of the present invention, the sowing comprises fertilizing. Fertilization is a farming method that promotes the growth of crops by supplying fertilizer components to soil or crops, preferably soil fertilization, irrigation fertilization and foliar fertilization. Soil fertilization is applied to the surface of the soil by spraying solid fertilizers such as Home Green, Cosmocote, Ortho and Magamp-K. Watering fertilization is water-soluble fertilizers such as Hyponex, Typo and Peter's, or water fertilizers such as North Salmon, Nargen and Amino Acid Solution, can be diluted in water to make the whole root wet. Foliar fertilization is sprayed with dilute liquid fertilizer on the leaves for quick recovery in the case of deficient symptoms in the leaves and stems due to poor root absorption due to poor root function or lack of special fertilizers. Is the way.

The fertilization may include fertilization, fertilization and fern. Gibi is a fertilizer for perennial plants, such as before planting crops or roots of bulbous roots. It is usually carried out in winter, and fertilization is also called manure, and it refers to fertilizers when crops are growing. Gibi fertilizes organic fertilizers with long-lasting, less-slow, slow-release effects, and slow-release (fertile) properties, while fertilizers are prone to lack of fertilizer. It is common to give mineral fertilizers with fast fertilizers near the surface. In addition, the fertilizer is a fertilizer near the root of the tree or when the fertilizer is applied to the soil, or on the surface of the soil. When it is watered or when it rains, it gradually melts and absorbs into the roots, making it easy to supply fertilizer. The effect is well characterized.

As used herein, the term “fertilizer” refers to a nutritional substance that is sprayed to increase the productivity of the land so that plants grow well. Also called manure, it is a general term for fertilizing land and promoting the growth of vegetation. The main components of fertilizers are nitrogen, phosphoric acid, potassium, sulfur, calcium and magnesium, and natural fertilizers and chemical fertilizers. Among the inorganic elements supplied from the soil, plants need a large amount, and nitrogen, phosphorus and potassium are easy to lack. This is particularly important as the 'three elements of fertilizer'. The next most likely element to be lacking after these three elements is calcium. Nitrogen, phosphorus, potassium, and calcium are sometimes referred to as 'four elements of fertilizer'. All crops absolutely require three elements of fertilizer to harvest a certain yield, but the amount depends on the type of crop. Some of this need comes from soil, irrigation water or rainwater, even if it is not fertilizer. The amount of these three naturally supplied components is more rice fields than fields.

The soil microbial agent of the present invention utilizes the function of the useful soil microorganisms to increase the supply of crop nutrients in the soil, or to provide a specific microbial agent purely cultured to the soil for the purpose of enhancing the effectiveness of crop nutrients in the soil. Say. Bacillus, Clostridium, Trichoderma, Azotobacter and Rhizobium for soil improvement, Pseudomonas, Bacillus, Gliocladium, Trichoderma, Non-pathogenic Fusarium, Non-pathogenic Erwinia, Streptomyces, Xanthomonas, VA Mycorrhizal fungus, Azotobacter genus and Verticillium genus, Streptomyces genus, Pseudomonas genus, Bacillus genus, Clostridium genus and Thermus genus to promote organic degradation, VA mycobacterium, Azotobacter genus, Bacillus genus, Rhizobium genus, Trichoderma genus Candida genus, Frankia genus, Azospirillum genus and Bradyrhizobium genus, photosynthetic bacteria, heat-resistant actinomycetes, Rhizoctonia genus and Pseudomonas genus to promote growth, Bacillus genus and Pasteuria genus for pest control, Epicoccosorus genus and Dendryphiella genus for weeding .

The liquid fertilizer of the present specification means that the trace urea complex fertilizer product including selenium is prepared by diluting with water for foliar fertilization.

As used herein, the term "foliar fertilization" means a method of artificially absorbing the ingredients necessary for the growth of the crops through the leaves of the crop, and through the pores (氣孔) while at the same time the photosynthesis and respiratory action and at the same time carbon dioxide gas and Oxygen is absorbed and exchanged. In addition to these gases, the materials absorbed by the pores usually include nutrients or other inorganic and organic substances that are absorbed from the roots, where the absorbed material is used in the body for normal growth. Foliar fertilization is more effective in distributing nutrients when the nutrient absorption function of roots is severely impaired due to the occurrence of hydrogen sulfide (H ₂ S) in paddy soils or in case of pest or applied nutrients inevitably compared to soil fertilization. It is an effective fertilization method. In addition, in the case of trace elements, the practicality is very high because the nutrients required by plants can be satisfied by spraying once or 2-3 times. Leaf absorption is known to be a complex action of diffusion through the first pore, permeation through micropaths developed in the epidermal cells of the second leaf, and electrical attraction of the third epidermal cell layer. In the case of trace element deficiency in crops, foliar spraying is more effective and less economical than giving the deficiency to soil. For example, foliar application of rice and citrus fruits with manganese, zinc, and iron deficiency may be effective in foliar spraying, and foliar spraying may be faster than soil fertilization, resulting in frostbite, feng shui and pests. Receive foliar fertilization when the growth is bad is effective. In addition, if the roots are harmed by pests, moist and reducing harmful substances, if the damage of the roots is not severe, growth is improved by foliar spraying and the damage is recovered to some extent, and vines such as watermelon and melon crawl and spread on the ground. In case of difficulty in using manure or for special purposes such as quality improvement, foliar fertilization can be performed.

According to a preferred embodiment of the present invention, the foliar fertilization is characterized in that it is carried out five times at intervals of two weeks. Preferably, the selenium concentration used for fertilization is 2-10 mg / kg, more preferably 2-6 mg / kg, and most preferably 4 mg / kg.

As used herein, the term “ground core” refers to the intellect, and the grounded soil means that soil microorganisms are in harmony, and a large number of them exist, and there is sufficient soil nutrients for plants to grow healthy and absorb nutrients. Refers to soil that can grow well and grow roots.

According to another aspect of the present invention, the present invention provides a selenium-containing spinach through the method of cultivating the selenium-containing spinach using the selenium-containing fertilizer.

The features and advantages of the present invention are summarized as follows:

(a) The present invention provides a novel method for growing functional spinach containing high concentrations of selenium.

(b) According to the present invention, it is possible to provide a spinach having an increased selenium content of at least 19.4 times by fertilizing fertilizer containing selenium.

(c) By using the special fertilizer of the present invention to absorb the organic selenium through the plant, and also combined with the special mineral components, there is an advantage to enable the production of functional and eco-friendly agricultural products by combining the organic method.

1 is a schematic diagram showing a test group fertilizing fertilizer containing selenium and a control group not fertilizing fertilizer containing selenium.
* Original Selenium Concentration: 2,000 ppm
* By region: Ⅰ, written Seoho-ri; II, Namsang Sangari; III, Shenzhen-ri, Namhae-eup
* Classification by concentration: A, control (no treatment); B, the original treatment 500 times dilution treatment.
Figure 2 is a view showing that the cultivation (cultivate) and ridge (ridge and furrow) in the field prior to the cultivation of spinach.
Figure 3 is a schematic diagram showing the schedule of spinach cultivation and fertilization. The harvest season followed the practices from mid-January to the end of March the following year, and fertilization was carried out five times at two-week intervals.
Figure 4 is a diagram showing the site of fertilizer fertilization using a field and tillage dilution of a suitable amount by dividing the fertilizer containing selenium to a predetermined concentration for each compartment by foliar fertilization method.
5 is a graph showing the growth difference of spinach according to foliar fertilizer fertilizer containing selenium.
(a) chlorophyll content, (b) leaf length, (c) leaf width, (d) root length, (e) muscle width
* By region: Ⅰ, written Seoho-ri; II, Namsang Sangari; III, Shenzhen-ri, Namhae-eup
* Classification by concentration: A, control (no treatment); B, 500 times dilution
6 is a diagram showing the growth difference of spinach according to foliar fertilizer fertilizer containing selenium.
(a): early growth; (b): late growth
* By region: Ⅰ, written Seoho-ri; II, Namsang Sangari; III, Shenzhen-ri, Namhae-eup
* Classification by concentration: A, control (no treatment); B, the original treatment 500 times dilution treatment.
7 is a graph showing the soil analysis results of the spinach cultivation area.
* By region: (a), written west hori; (b), Namsang Sangari; (c), Shenzhen-ri, Namhae-eup
* Unit: pH (1: 5), organic matter (g / kg), effective phosphoric acid (mg / kg), alveolar cation (cmol + / kg), effective silicic acid (mg / kg)
8 is a graph showing the results of analysis of organic selenium content contained in spinach after fertilizing fertilizer containing selenium.
* Regional classification: Ⅰ, written west hori; II, Namsang Sangari; III, Shenzhen-ri, Namhae-eup
* Classification by concentration: A, control (untreated); B, 500 times dilution

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example

Example 1 Sowing and Fertilization

Spinach four season varieties (Haesong Seed) were each directly 1.5 kg (500 g / bag) in 1,000 m² of open ground in Seoho-ri, Nam-myeon Sanga-ri, Nam-myeon, and Shenzhen-ri, Namhae-gun, Gyeongnam. Fertilization amount is 3 tons of organic compost (practice) per 1,000 m2, 20 mixed organic milk fertilizers (Tensim Yu-bak, winning cooperative, catalog No. 08-Organ-3-042, 20 ㎏ / bag) and lime , Tong Yang Industry Co., Ltd., 20 ㎏ /]] 10 bags to fertilize the whole amount before tilling tillage, or can be carried out using a variety of methods known in the art. The design was carried out by dividing the packaging as shown in FIG. 2 (cultivate and ridge and furrow of four seasons of spinach cultivation packaging) prior to fertilization.

Example 2: Fertilizer Application Method with Selenium

After 3-4 weeks of sowing spinach liquid fertilizer containing selenium was applied to foliar fertilization five times at intervals of two weeks with the program of Figure 3 (Fig. 4). Foliar fertilization can be carried out using a variety of methods known in the art. For selenium foliar fertilization test, selenium farm (Carouss, foliar application cost, Se 2,000 mg / kg) microurea complex fertilizer product was used. The control group was fertilized with 60 liters of water, and the test group was diluted with selenium palm water. 540 L was fertilized. The foliar fertilization concentration of selenfam was treated by diluting to 500: 1 (4 mg / kg).

Example 3: Growth test of spinach fertilized with fertilizer containing selenium

Normal growth of chlorophyll fertilized with fertilizer containing selenium (chlorophyll content, leaf length, leaf width, root length, root circumference and root condition) was sown and 60-70 days after primary and 90-105 days after 2 After randomly selecting subjects by car, 500g of the sample was collected, washed with distilled water, and measured. Chlorophyll content was measured non-destructively on the leaves of the specimen using a chlorophyll meter (Chlorophyll Meter, SPAD-502, Minolta, Japan; Photosyn. Res. 46 (3): 467-472. 1995). Samples were randomly selected and statistically repeated at least 9 times. In addition, visual identification and growth measurements were repeated three or more times for each sample in order to determine the growth, weakness, and quality response of spinach treated with selenium (27 samples per analysis item). The growth characteristics of spinach were examined according to the Agricultural Science and Technology Research Analytical Criteria (Rural Development Administration, 2003). All results were tested by the Duncan's multiple range test (DMRT) at α = 0.05 using the SAS program.

When foliar fertilizer containing selenium was applied, chlorophyll, leaf width, leaf length, root length, thickness, and root length were not significantly different among the first and second harvested individuals. Individuals were found to be relatively well-grown. Analysis of the first and second harvested subjects showed chlorophyll (54.8-60.7 SPAD), leaf length (8.83-12.83 cm), leaf width (3.67-5.17 cm), root length (9.00-14.67 cm), muscle width (0.43-0.83) cm) and triceps (usually-many) showed no significant differences between regions (Tables 1, 2 and 5a-e). As a result of the growth analysis of the first harvesting individuals fertilized with selenium fertilizer, it was confirmed that no damage was occurred and there was no problem in the growth of spinach (FIGS. 6A-B).

 First Harvest Spinach Growth (Unit: SPAD, Cm) division chlorophyll Leaf Leaf width Rootstock Muscle width Fine hair I-A 59.9 11.17 4.5 9.17 0.6 Above average I-B 60.1 12.57 5.33 10.67 0.83 plenty II-A 58.8 11.66 4.33 13.00 0.57 Above average II-B 54.8 11.83 4.83 11.67 0.60 plenty III-A 57.6 8.83 3.67 9.00 0.64 Low III-B 58.5 10.33 3.83 11.33 0.43 Above average

* By region: Ⅰ, written Seoho-ri; II, Namsang Sangari; III, Shenzhen-ri, Namhae-eup

* Classification by concentration: A, control (no treatment); B, 500 times dilution

* Statistics treatment population (n): Chlorophyll 9, others 27.

Second Harvest Spinach Growth (Unit: SPAD, Cm) division chlorophyll Leaf Leaf width Rootstock Muscle width Fine hair I-A 59.4 11.00 4.20 10.70 0.70 plenty I-B 60.7 11.67 5.33 10.50 0.80 plenty II-A 59.5 10.17 3.83 12.50 0.57 Above average II-B 58.9 12.83 5.17 14.67 0.73 plenty III-A 58.3 10.83 4.00 13.00 0.43 Above average III-B 58.6 10.00 4.00 12.00 0.50 Low

* By region: Ⅰ, written Seoho-ri; II, Namsang Sangari; III, Shenzhen-ri, Namhae-eup

* Classification by concentration: A, control (no treatment); B, 500 times dilution

* Statistics processing population (n): Chlorophyll 9, others 27

Example  4: Soil Analysis of Spinach Cultivation Area

For soil analysis of spinach cultivation area, soil samples were collected from 1,000 m² of open ground in Seoho-ri, Nam-myeon, Sanga-ri, Nam-myeon and Shenzhen-ri, Namhae-eup before and after the second seeding. Since the soil seeded with spinach was uniform in fertility, the soil of topsoil was slightly removed from 30 places, and then 600 g of 10-15 cm of topsoil was collected, mixed, spreaded thinly on vinyl, and air dried indoors. The air dried sample is removed from the contaminant by using a net, and only soil of uniform size is recovered as an analytical sample, so that acidity, organic matter, effective phosphoric acid, replaceable cations (calcium, magnesium and potassium), EC, effective silicic acid and calcification requirements Used for analysis.

As a result of the soil analysis, the acidity (pH5.8-6.4 / 5.0-5.8) was compared before the sowing date of the spinach (before fertilizing fertilizer containing selenium) and after the second harvest (after fertilizing fertilizer containing selenium). , Organic matter (23-38 / 24-34 g / kg), effective phosphoric acid (140-283 / 181-299 mg / kg), potassium (0.3-1.5 / 0.5-2.7 cmol + / kg), calcium (3.5-10.1 / 6.4-10.0 cmol + / kg), magnesium (1.0-3.6 / 1,2-4.0 cmol + / kg) and effective silicic acid (10.0-98.0 / 20.0-90.0 mg / kg) (FIGS. 7A-C). In general, in the case of cultivation of spinach field before and after foliar fertilizer containing selenium, the ground core was found to be “strong” in “suitable” and the lack of phosphoric acid and silicic acid in the soil. In general, base manure during cultivation of open field spinach is based on the recommended amount (8.1-13.1 kg of compound fertilizer (10-7-25), 3.5-6.8 kg of urea and 12.2-20.0 kg of soluble phosphorus). The manure was confirmed to be somewhat adjusted according to the growth conditions.

Example  5: Analysis of Selenium Content in Spinach Cultivated Soil

To analyze selenium content in spinach-cultivated soils, soil samples were collected from 1,000㎡ of open ground in Seoho-ri, Nam-myeon, Sanga-ri, Nam-myeon and Shenzhen-ri, Namhae-eup before and after the second seeding. 0.2 g of soil sample was treated with mixed acid (5 ml of nitric acid + 5 ml of sulfuric acid), heated (160 ° C / 6 hr), cooled down to gel state, evaporated to a gel state, and filled with distilled water to an initial volume. It was analyzed by pottery (ICP- Mass spectrometer, ELAN 6100, Perkin Elmer, USA; EPA Methods 200.8, Rev. 5.4.).

Selenium was not detected in the soil before foliar fertilization of selenium-containing fertilizers in Seoho-ri, Nam-myeon Sanga-ri, Namhae-gun, Shenzhen-ri, Namhae-gun.

Example  6: Analysis of Spinach-Containing Organic Selenium Content

The selenium content in spinach was sown, 60-70 days after the first and 90-105 days after the random selection of the subjects by zone, 500g each collected for repeated analysis and washed with distilled water to dry the analysis sample Ready. 0.2 g of the obtained spinach leaf sample was treated with mixed acid (5 ml of nitric acid + 5 ml of sulfuric acid), heated (160 ° C./6 hr), cooled to a gel state, evaporated to a gel state, filled with distilled water, and then inductively coupled plasma. The mass spectrophotometer (ICP- Mass spectrometer, ELAN 6100, Perkin Elmer, USA; EPA Methods 200.8, Rev. 5.4.) Was analyzed.

The selenium content in the spinach of the first sample of the control group without foliar fertilizer containing selenium was 0.082-0.258 mg / kg (West Seo-ri), 0.077-0.206 mg / kg (Southern Sangari) and 0.081-0.123 mg / kg. (Shenzhen-ri, Namhae-eup), and the selenium content in the spinach of the second sample was 0.042-0.221 mg / kg (Seoho-ri, Seomyeon), 0.447-1.012 mg / kg (Namsang Sangari), and 0.429-0.569 mg / kg (Shenzhen-ri, Namhae-eup). In addition, the selenium content in the spinach of the primary sample of the test group foliar fertilizer containing selenium (initial fertilization; three times) was 0.467-0.816 mg / kg (Seoho-ri) and 0.691-0.899 mg / kg (Southern Sangari) ) And 0.550-0.755 mg / kg (Shenzhen-ri, Namhae-eup), and the selenium content in the spinach of the second sample (late fertilization; five times) was 1.474-2.595 mg / kg (Seoho-ri, Seoseo-ri), 1.356-3.356 mg / kg (Southern Noodles) Sangari) and 2.441-3.090 mg / kg (Shenzhen-ri, Namhae-eup) (Table 3).

In the second sample of spinach without fertilizer containing selenium, the selenium content (0.429-1.012 mg / kg) was higher than that of the spinach fertilizer containing selenium in Nammyeon and Namhae-eup. It is considered that the fertilizer containing selenium was accumulated in the control area due to environmental factors (wind) during fertilization. The selenium content in the soil of Sinanseomcho and Pohangcho used as another control group was 0.100 mg / kg and 0.092 mg / kg, respectively. The selenium content in Sinanseomcho and Pohangcho soils was similar to that of the control group in Namhae.

Organic selenium content of spinach fertilized with selenium fertilizer increased more than 5.5 times (0.128 / 0.697 mg / kg) by region at the beginning of fertilization compared with spinach without fertilized fertilizer containing selenium. Increased by more than 19.4 times (0.125-2.423 mg / kg) (Fig. 8).

Organic Selenium Concentration in Spinach by Leaf Fertilization (Unit: ㎎ / ㎏) division I-A I-B II-A II-B III-A III-B Sinan Island Pohang Early fertilization (3 times) 0.082 0.467 0.077 0.691 0.081 0.550 0.100 0.092 - - - - - - 0.258 0.816 0.206 0.899 0.123 0.755 Early fertilization (5 times) 0.042 1.474 0.878 1.356 0.429 2.441 - - - - - - 0.221 2.595 1.012 3.356 0.569 3.090

* By region: Ⅰ, written Seoho-ri; II, Namsang Sangari; III, Shenzhen-ri, Namhae-eup

* Classification by concentration: A, control (no treatment); B, 500 times diluted fertilizer treatment solution containing selenium

* Test throughput: 0.2 g / 500 g.

Claims (8)

Method of growing spinach containing 1.356-3.356 mg / kg selenium, comprising the following steps:
(a) sowing spinach seeds; And
(B) cultivating spinach while fertilizing fertilizer containing selenium from 3 to 4 weeks after sowing, diluting the diluted solution in water so that the selenium concentration is the final 2-10 mg / kg five times at two-week intervals.
According to claim 1, The selenium is K ₂ SeO ₃ , K ₂ SeO ₄ , Na ₂ SeO ₃ , Na ₂ SeO ₄ , CaSeO ₃ , CaSeO ₄ , SeO ₃ , H ₈ N ₂ O ₄ Se and H ₈ N ₂ Cultivation method, characterized in that any one or more selenium selected from the group consisting of O ₃ Se.
The cultivation method according to claim 1, wherein the sowing comprises fertilizing.
The method of claim 1, wherein the fertilizer comprises a soil microbial agent.
delete The method of claim 3, wherein the fertilizer is fertilization, fertilization or fern.
delete A spinach containing selenium produced by the method of any one of claims 1 to 4 and 6.

Images