KR101364752B1 - Method for prepairing food using abalone, seaweed and wild plants - Google Patents

Abstract

The present invention relates to a method of manufacturing food using abalone, seaweed and wild grass, more specifically, abalone functional having the effect of hydrolyzing abalone to prevent cognitive dysfunction or memory loss, preventing learning deterioration and preventing or improving dementia The present invention relates to a method for preparing foods using abalone, seaweed and wild grass that can be prepared by enzymatic hydrolyzate and improve the palatability by mixing seaweed and wild grass extract of the abalone.
Food production method using abalone, seaweed and wild grass according to the present invention, (a) pre-treatment of washing the abalone, removing foreign substances, shell removal, and (b) the edible portion separated in the first step grinder A second step of pulverization using the second step, (c) a third step of enzymatic hydrolysis by adding a proteolytic enzyme to the pulverized product prepared in the second step, and (d) the abalone enzyme prepared in the third step A fourth step of materializing the abalone enzymatic hydrolyzate by inactivating the enzyme through heat treatment of the red hydrolyzate and removing the remaining insoluble material, and (e) seaweed (seaweed, seaweed, kelp) and wild grass (bokbunja, (5) a fifth step of pre-treatment of raw materials, such as Angelica gigas and Ogapi), and (f) a sixth step of extracting hot water by adding 10 times of water to each of the pre-treated raw materials of the fifth step or together, and (g) The extract obtained in step 6 is filtered and concentrated or dried It is characterized in that it comprises an eighth step of mixing the extracts obtained from marine algae and sanyacho seventh step and, (h) the upset enzymatic hydrolyzate and a sixth step obtained in step 4.

Description

METHODS FOR PREPAIRING FOOD USING ABALONE, SEAWEED AND WILD PLANTS

The present invention relates to a method of manufacturing food using abalone, seaweed and wild grass, more specifically, abalone functional having the effect of hydrolyzing abalone to prevent cognitive dysfunction or memory loss, preventing learning deterioration and preventing or improving dementia The present invention relates to a method for preparing foods using abalone, seaweed and wild grass that can be prepared by enzymatic hydrolyzate and improve the palatability by mixing seaweed and wild grass extract of the abalone.

Abalone belongs to the abalone family of the native mollusks of useful mollusks, and it is grown by feeding algae and is rich in minerals such as calcium and phosphorus, vitamin B ₁ , B ₂ and protein, and especially rich in taurine, soy sauce protection and fatigue recovery. It is a high-quality fish and shellfish that has a prophylactic effect against myocardial infarction. In Korea, it protects the tonic and kidneys, clears the lip, opens the stomach, controls the sea water, recovers from fatigue and nourishment tonic. It is known to be effective and is favored as a health food. In addition, abalone is known to have an excellent effect on the optic nerve fatigue symptoms, the shell of abalone is also used for conjunctivitis and cataracts because it is called Seok-myeong (石決明) in oriental medicine. Abalone has almost no processed food related technology due to its scarcity, but its production is increasing rapidly due to the development of aquaculture technology. In order to secure shelf life and to use it as a herbal medicine, consumption is gradually being expanded to the method of processing dried abalone, abalone porridge, red pepper paste abalone, and abalone gut salted seafood.

Seaweed is a red algae plant and belongs to the red algae raw red algae and Kim Mok lamiaceae. It is also called Haitai or Haiui. It is one of the representative algae in Korea along with seaweed and kelp. Kim's nutritional ingredient is an alkaline food rich in minerals containing more than 30% protein and less than 1% fat. It is a health food containing a lot of essential amino acids such as methionine, threonine, leucine, isoleucine, lysine and valine. In particular, it is rich in vitamins A, B, D, and E, and known to be closely related to brain development, vitamin B ₂ is much higher than other plant foods. Seaweed contains high amounts of dietary fiber and high amounts of polysaccharides and taurine, which are good at improving cholesterol, which causes arteriosclerosis.

Kelp is rich in many alkaline metal ions such as potassium, sodium, calcium, and magnesium, which not only helps to balance the diet of modern people, but also is a good source of minerals containing more than 4,000 ppm of iodine. In addition, alginic acid, one of the main constituents of kelp, has been found to have various effects such as cholesterol lowering function, prevention of harmful heavy metal absorption and release as well as dietary fiber. Laminine, a low-molecular nitrogen compound in kelp, has been shown to have a blood pressure-lowering effect and is an excellent food source containing a lot of taurine.

Sanyacho is also widely used as a herbal medicine in herbal medicine, and various researches are being conducted due to its focus on developing dementia and therapeutic materials due to its diversity and functionality.In addition, acetylcholine esterase inhibitor, β-amyloid, brain accumulation and cerebral blood flow Research is being conducted on various aspects such as improvement, oxidative stress inhibitory effect and estrogenic effect.

Angelica is a plant in the genus Angelica, whose stem is dark green, 1.0-1.5 m tall, the flowers are light purple, and the flowering period is from August to September. It is cultivated for use as a herbal medicine in Korea, China and Japan. Angelica is known to be effective in treating anemia, sedation, anticancer, analgesic suppression, wound healing, and diabetic hypertension. It also contains many functional ingredients such as nodakentin, decursin, decursinol, umbelliferon, and β-sitosterol. In the dementia-induced model treated with intraventricular β-amyloid 1-42, Angelica significantly increased the escape latency in the passive avoidance test, showed dose-response, and the longer the duration of administration, the higher the effect.

Bokbunja is a deciduous shrub belonging to the Rosaceae family, reaching 3m in height, growing in sunny areas at the foot of the southern and central regions, including Jeju Island, Korea. The mountain stalk is at the end of its branches, hairy, and blooms in May-June, ripens red in July-August, but later turns black. In general, bokbunja refers to immature fruit, and has been used in oriental medicine, birth, jisin, genital, tonic, and wool. According to the study of each part of bokbunja, phenolic compounds and flavonoids were identified using leaves, and two flavan-3-ols, one proanthocyanidin and one ellagitanin were isolated from the stem. In addition, studies on antioxidant, increased immune activity, hepatitis B virus (HBV) inhibition, anticancer activity, anticancer and antistress effects have been reported.

Ogapi is the root, stem and bark of the algae tree or other cognate, belonging to the family Arboraceae, tubular or semi-tubular, usually 5-10 cm long, 5-8 mm in diameter, 1 mm thick, and its outer surface is tan-dark gray. It has thorns or marks in some places, and there are off-white spots on the bark of relatively young branches. Ogapi has been used as a metabolic accelerator for divergence and cleft, according to the classics such as Dongbobogam, Chinese herbal medicine cultivation, Shinnongboncho, and Herbal Wood, especially nourishment, tonic, gangjeong, sonic, Jinkyung, muscular pain, acidic abdominal pain, and painful pain. Because of the efficacy of the tonic is mainly known to treat neuralgia, stroke, hypertension, diabetes, rheumatoid arthritis and the like.

Geriatric dementia causes severe brain atrophy and brain cell damage, resulting in a decrease in memory and cognitive function, and a dramatic decrease in abstract thinking. It is a representative degenerative chronic brain disease.

However, senile dementia is involved in the metabolism of antipsychotics, antidepressants, antianxiety agents, anticonvulsants, nootropics, and neurotransmitters because the exact cause of the disease is not well understood. The only symptomatic therapy is to treat drugs such as enzyme blockers and calcium blockers according to the condition of the patient. Agonists for the muscarinic acetylcholine receptor, acetylcholine production promoter, and acetylcholinesterase based on the research hypothesis that various cognitive dysfunctions such as memory loss and learning loss caused by senile dementia are mainly caused by damage of acetylcholine neurons in the base of cerebral base. Many drugs have been researched and developed to enhance the function of acetylcholine neurons according to various mechanisms of action such as (AChE) inhibitors. The active research and development of senile dementia improvers is the study of AChE inhibitors. All currently available drugs such as tacrine, rivastigmine, donepezil, and galantamine are based on acetylcholinesterase inhibitory effects.

Recognized as described above of the present invention, the object of the present invention is the abalone enzymatic harmless to the human body having the effect of preventing or improving cognitive impairment or memory loss, lowering of learning ability by enzymatic hydrolysis of abalone. Method of preparing foods using abalone, seaweed and wild grass that prepare hydrolysates and prepare functional foods for the prevention and improvement of cognitive impairment, dementia by mixing seaweed, kelp extract, Angelica, bokbunja, and ogapi extract with the active ingredients It is to provide.

Food production method using abalone, seaweed and wild grass according to the present invention in order to achieve the above object, (a) pre-treatment of washing the abalone, removing foreign substances, shell removal, and (b) the first step A second step of pulverizing the decorated site separated in step 2 using a grinder, (c) a third step of enzymatic hydrolysis by adding a proteolytic enzyme to the pulverized product prepared in the second step, and (d) A fourth step of materializing the abalone enzymatic hydrolyzate by inactivating the enzyme by heat treatment of the abalone enzymatic hydrolyzate prepared in the third step and removing the remaining insoluble material, and (e) laver, seaweed and kelp Seaweed and bokbunja, and the wild grasses containing the Angelica and Ogapi containing the fifth step of washing, removing foreign matters, and (f) hot water by adding 10 times the water or each of the pre-treated seaweeds and wild grasses of the fifth step 6th to extract Step 7, (g) the seventh step of filtration and concentration or drying the extract obtained in the sixth step, and (h) mixing the abalone enzymatic hydrolyzate obtained in the fourth step and the seaweed and wild grass extract obtained in the seventh step And an eighth step.

In addition, the method of preparing food using abalone, seaweed and wild grass according to the present invention, wherein the third step is any one selected from the group consisting of alkalase, neutrase protamex, flavozyme as the protease Using one or more enzymes, the sixth step, seaweed and wild grass extract is characterized in that the hot water extraction at 121 ℃.

In addition, the method of manufacturing food using abalone, seaweed and wild grass according to the present invention, the eighth step is mixed with abalone enzymatic hydrolyzate and seaweed and wild grass extract in the form of liquid, granules, pills, tablets It is characterized by manufacturing.

The method for producing food using abalone, algae and wild grass according to the present invention according to the above configuration, abalone hydrolyzate and algae, wild seaweed having the effect of preventing or improving cognitive impairment or memory loss, learning ability and dementia It has the advantage of preparing an extract and a functional food having dementia preventing activity containing the active ingredient.

1 is a view showing a flow of a method of manufacturing food using abalone, seaweed and wild grass according to the present invention
2 is a diagram showing the antioxidant activity of the abalone enzymatic hydrolyzate and seaweed and wild grass extract in the method of preparing food using abalone, seaweed and wild grass
3 is a view showing the anti-dementia prevention activity of food (composition) prepared by using the enzymatic hydrolyzate and seaweed and wild grass extract of abalone according to the method of preparing food using abalone, seaweed and wild grass according to the present invention

Abalone has a high content of amino acids, especially glutamic acid, arginine, histidine, tyrosine and taurine, and is rich in vitamins, calcium, phosphorus, iron and iodine, and is a healthy food for growing children, pregnant women, and the elderly. .

Sanyacho is also widely used as a herbal medicine in herbal medicine, and various researches are being conducted due to its focus on developing dementia and therapeutic materials due to its diversity and functionality.In addition, acetylcholine esterase inhibitor, β-amyloid, brain accumulation and cerebral blood flow Various effects such as improvement, oxidative stress inhibitory effect and estrogenic effect have been demonstrated.

Seaweeds have high levels of dietary fiber, minerals and functional polysaccharides, and their physiological effects continue to increase functional research and utilization, including lowering cholesterol, preventing cancer, diabetes, obesity, and constipation.

Currently known neurotransmitters include amino acids themselves (glutamic acid, glycine, γ-aminobutyric acid (GABA), taurine, etc.), amines (dopamine, nopaadrenaline, serotonin, acetylcholine, etc.) and peptides (endorphins, vasopressin, etc.). Substances (nitrogen monoxide, carbon monoxide). Amino acids are a synthetic material of neurotransmitters in the brain and greatly affect concentration and cognitive function. It has been reported that tyrosine is a source of catechol amines that excite the brain and increases work efficiency by reducing fatigue and drowsiness under stress conditions. Histidine is a raw material of histamine, and it activates the histamine nervous system involved in arousal, which may prevent drowsiness. Branched-chain amino acids (leucine, valine, isoleucine BCAA) inhibit serotonin, which inhibits the intake of other aromatic amino acids and causes drowsiness. It is an amino acid that is expected to sustain the concentration by inhibiting the production of.

In the brain, the amount of neurotransmitters is balanced to maintain healthy brain function. In order to maintain this balance, it is necessary to ensure that the delivery material is quickly supplied into the brain as needed. If the supply is insufficient, the intake of amino acids is also necessary to maintain brain function because nerve signals are not transmitted smoothly and various neurological abnormalities occur.

Abalone enzymatic hydrolyzate in the method of preparing food using abalone, seaweed and wild grass according to the present invention can be obtained from the process consisting of abalone washing and pretreatment, enzyme treatment, enzyme inactivation, filtration, concentration or drying. All. In addition, the hot water extract of seaweed and wild grass can be obtained from the process consisting of raw material washing and pretreatment, hot water extraction, filtration, concentration or drying.

Specifically, a method of preparing food using abalone, seaweed and wild grass according to the present invention is as follows.

1 is a view showing the flow of a method of manufacturing food using abalone, seaweed and wild grass according to an embodiment of the present invention, referring to Figure 1, abalone, seaweed and wild grass according to an embodiment of the present invention The manufacturing method of the used food,

(a) Pre-treatment of washing the abalone, removing foreign matters and removing the shells.

(b) a second step of grinding the decorative part separated in the first step by using a grinder.

(c) a third step of enzymatic hydrolysis by adding proteolytic enzyme to the milled product of the second step.

The proteolytic enzyme uses an alkalase, and one or two or more enzymes selected from the group consisting of Neutrase protamex and flavozyme can be used.

(d) a fourth step of materializing the abalone enzymatic hydrolyzate by inactivating the enzyme by heat treatment of the abalone enzymatic hydrolyzate prepared in the third step to remove the remaining insoluble material.

(e) Fifth step of pretreatment to remove raw materials such as seaweed (seaweed, seaweed, kelp) and wild grass (bokbunja, Angelica, Ogapi).

(f) Sixth step of extracting hot water by adding ten times of water to each or together the pretreated raw material of the fifth step.

(g) a seventh step of filtering and concentrating or drying the extract obtained in the sixth step.

(h) characterized in that it comprises an eighth step of mixing the abalone enzymatic hydrolyzate obtained in the fourth step and the seaweed and wild grass extract obtained in the seventh step.

Method for producing a functional food of dementia prevention activity using the abalone enzymatic hydrolyzate and seaweed and wild vinegar extract according to the present invention is the above-described enzymatic hydrolyzate and extract of the form of any one of liquid, granules, tablets, pills It is characterized in that the manufacturing.

In addition, the present invention was confirmed that the brain function neurotransmitter inhibitory activity and antioxidant activity of the abalone enzymatic hydrolyzate and seaweed and wild grass extract.

Hereinafter, a method for preparing abalone enzymatic hydrolyzate, a method for preparing seaweed and wild grass extract, and a method for preparing food using abalone, seaweed and wild grass will be described in detail.

Example 1 Preparation of Abalone Enzymatic Hydrolyzate

The preparation method of the abalone enzymatic hydrolyzate of the present invention is as follows. Wash the abalone and remove the shells, add 800 mL of distilled water per 1 kg of meat and intestine together, then grind completely with a meat grinder, and add 1.2 g of protease, Alcalase enzyme, The reaction was carried out for 4 hours in a stirred incubator to obtain an enzymatic hydrolyzate. The obtained hydrolyzate was heated at 90 ° C. for 15 minutes to inactivate the activity of the alcalase enzyme, and then centrifuged at 3,000 rpm and filtered to remove insolubles, thereby obtaining only a supernatant. The supernatant was heat-treated at 90 ° C. for 20 minutes to be concentrated or used to prepare a functional food material.

Example 2 Preparation of Abalone Hot Water Extract

Preparation of hot water extract for comparison with the abalone enzymatic hydrolyzate is as follows. The fresh abalone was washed and ground together with meat and intestine, and then ground using a grinder. 250 g of abalone crushed powder was added to the extractor for 1 L of water, and the extraction temperature was 120 ° C. and the extractor pressure was 1.0 kgf / cm ² . Extraction was performed by extracting the mixture for 3 hours under filtration and filtering.

Example 3 Preparation of Seaweed Extract

The preparation method of the seaweed extract in the present invention is as follows. Remove foreign substances of seaweed (seaweed, seaweed, kelp) and add hot water of 10L per 1kg to extract hot water at 121 ℃ for 3 hours. The hot water extract was filtered using a filter and concentrated or dried under reduced pressure to prepare an algae extract.

Example 4 Preparation of Sanyacho Extract

The production method of wild vinegar extract of the present invention is as follows. Sanyacho, such as bokbunja, Angelica, and Ogapi, removes foreign substances and adds 10L of purified water per 1kg to extract hot water at 121 ° C for 3 hours. The hot water extracted was filtered using a filter, and concentrated or dried under reduced pressure to prepare a wild leaf extract.

Example 5 Antioxidant Activity

Antioxidant activity of the abalone enzymatic hydrolyzate, seaweed and wild vinegar hot water extract were measured as follows. 0.4 mL of each extract or enzymatic hydrolyzate was taken in a test tube, mixed with 1.6 mL of 0.1 M DPPH ethanol solution for 10 minutes in the dark, and the change in absorbance of the solution was measured at 517 nm. The measured absorbances were calculated according to the following equation and expressed as free free radical scavenging ability to compare antioxidant activity. The comparison result is shown in FIG. In other words, the abalone enzyme was 93.4%, seaweed hot water extract 54.5%, seaweed 45.3%, kelp 44.7%, bokbunja 91.3%, Angelica 42.2%, Ogapi 38.7%.

Antioxidant activity (%) = 100- (absorbance of blank test-absorbance of sample) × 100 / absorbance of blank test)

Example 6 Determination of Brain Function Neurotransmitter Degrading Enzyme Inhibition of Dementia

In order to measure the cognitive impairment preventive activity of the abalone enzymatic hydrolyzate, brain neurotransmitter inhibitor activity was measured. In other words, Acetylcholinesterase (AChE) inhibition effect was measured according to the conventional method using AChE (E.C. 3.1.1.7) extracted from eel with acetylthiocholine as a substrate. Hydrolysis of choline acetyltransferase (ChAT) produces thiocholine, which reacts with DTNB to form new dithio compounds and thio anions of 2-nitrobenzoic acid. Since the anion thus produced is accurately detected by UV, the activity of the enzyme can be measured by measuring the production rate of this anion by UV.

Determination of brain function neurotransmitter degrading enzyme activity of dementia showed higher inhibitory effect on enzymatic hydrolyzate than abalone hot water extract, and even better than abalone hot water extract when mixed with abalone enzyme digest, seaweed and wild grass extract. appear.

Example 7 Determination of Amino Acid Content

Table 1 shows the results of analyzing the amino acids of the abalone hydrothermal extract and enzyme digestion. 5g of the amino acid was added to 50 mL of 70% ethanol was added to the reflux condenser and heated to reflux at 100 ℃ for 1 hour and filtered. The filtrate was concentrated under reduced pressure to 2 to 3 mL under 40 ° C, the concentrate and the concentrated water were washed with a small amount of distilled water, transferred to a separatory funnel, and 20 mL of diethyl ether was added to the bottom layer to be concentrated and dried. . The dried sample solution was dissolved in lithium citrate buffer (pH 2.2), and the volume was 25 mL and analyzed using an automatic amino acid analyzer. The amino acid content of the analysis is shown in Table 1.

amino acid Abalone hot water extract Abalone Enzyme Digest Aspartic acid 30.8 549.7 Threonine 39.0 364.4 Serine 37.6 1074.5 Glutamic acid 38.8 2071.0 Proline 33.0 495.7 Glycine 41.3 983.5 Valine 35.1 291.4 Methionine 31.9 1147.7 Isoleucine 31.1 907.7 Leucine 33.5 1936.2 Tyrosine 34.3 970.3 Phenylalanine 31.9 304.4 Lysine 41.7 1640.2 Histidine 34.4 92.1 Arginine 82.1 2508.4 Total 576.5 15,337.2

<Amino Acid Content of Abalone Hot Water Extract and Enzyme Degradation (mg / 100g)>

Example 8 Preparation of Functional Food Using Abalone, Seaweed, and Wild Grass

Preparation Example 1 Preparation of Liquid Composition Using Abalone, Seaweed, and Wild Grass

Abalone enzymatic hydrolyzate and seaweed and Sanyacho hot water extract are prepared by the above method and mixed in a mixing tank. Composition ratio of the composition is abalone enzymatic hydrolyzate 40%, palatinose 1%, seaweed calcium 0.5%, vitamin C 0.5%, kelp extract 10%, seaweed extract 8%, seaweed extract 8%, Angelica extract 10%, bokbunja extract It was prepared by mixing 10%, Ogai extract 10%, licorice extract 1.5%, grapefruit seed extract 0.5%. Each component was mixed with stirring to mix well, filtered and sterilized, and then packaged in a conventional retort pouch or PET container or glass bottle.

Preparation Example 2: Preparation of pill composition using abalone, seaweed, and wild grass

Preparation of the pill composition is 40% abalone enzymatic hydrolyzate, 2% palatinose, 0.5% algae calcium, 0.5% vitamin C, kelp extract powder 1%, seaweed extract powder 1%, seaweed extract powder 1%, donkey extract powder 1 %, Bokbunja extract powder 2%, Ogapi extract powder 1%, brown rice powder 30%, brewer's yeast 10%, glucose 10% were mixed in a mixer, molded using a ring automatic manufacturing machine and dried to prepare pills.

Preparation Example 3 Preparation of Granule Composition Using Abalone, Seaweed, and Wild Grass

Preparation of granule composition is 40% abalone enzymatic hydrolyzate, 2% palatinose, 0.5% algae calcium, 0.5% vitamin C, kelp extract powder 1%, seaweed extract powder 1%, seaweed extract powder 1%, donkey extract powder 1 %, Bokbunja extract powder 2%, Ogapi extract powder 1%, brown rice powder 30%, brewer's yeast 10%, glucose 10% was mixed in a mixer, molded using a cylindrical granulator, dried and selected to prepare a granule.

Although the composition ratio of the additives included in each of the above compositions was preferably mixed with an example of a suitable ingredient for health food, the composition ratio may be arbitrarily changed, and other raw materials may be prepared according to a conventional method. It can be used for manufacture.

The present invention is to prepare an enzymatic hydrolyzate abalone, confirm that the prepared hydrolyzate is effective in inhibiting dementia prevention neurotransmitter degrading enzyme and antioxidant activity, and active ingredient and palatability of seaweed extract and wild vegetable extract It was confirmed that this excellent functional food for preventing or improving cognitive dysfunction could be obtained.

The method of preparing food using abalone, algae and wild grass described above and shown in the drawings is only one embodiment for carrying out the present invention, and should not be construed as limiting the technical idea of the present invention. The scope of protection of the present invention is defined only by the matters set forth in the claims below, and the embodiments which have been improved and changed without departing from the gist of the present invention will be apparent to those skilled in the art. It will be said to belong to the protection scope of the present invention.

Claims (3)

(a) the first step of pretreatment of washing the abalone, removing foreign substances and removing the shell;
(b) a second step of grinding the decorated part separated in the first step by using a grinder,
(c) a third step of enzymatic hydrolysis by adding proteolytic enzyme to the milled product prepared in the second step;
(d) a fourth step of materializing the abalone enzymatic hydrolyzate by inactivating the enzyme by heat treatment of the abalone enzymatic hydrolyzate prepared in the third step and removing the remaining insoluble material;
(e) a fifth step of washing and removing foreign substances containing seaweeds containing seaweed, seaweed and kelp, and wild grasses containing bokbunja, Angelica and Ogapi;
(f) a sixth step of extracting hot water by adding 10 times of water to each of the algae and wild grasses pretreated in the fifth step;
(g) a seventh step of filtering and concentrating or drying the extract obtained in the sixth step;
(h) a method for producing food using abalone, seaweed and wild grass, comprising an eighth step of mixing the abalone enzymatic hydrolyzate obtained in the fourth step with the seaweed and wild grass extract obtained in the seventh step. The method of claim 1,
The third step, using any one or more enzymes selected from the group consisting of alkalase, neutrase protamex, flavozyme as the proteolytic enzyme,
In the sixth step, seaweed and wild vinegar extract is a method of producing food using abalone, seaweed and wild grasses characterized in that the hot water extraction at 121 ℃. 3. The method according to claim 1 or 2,
The eighth step is mixed with abalone enzymatic hydrolyzate and algae and wild grass extract to produce a form of liquid, granules, pills, tablets, abalone, seaweeds and wild vegetables.

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