KR101311424B1 - Microorganism multiplying and mixing apparatus - Google Patents

Abstract

The present invention relates to a microorganism culturing and mixing apparatus for culturing and mixing various microorganisms, the incubator unit having a culture chamber partitioned to cultivate photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group, and photosynthetic bacteria And controlling the incubator unit to cultivate each microorganism belonging to the cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group according to a predetermined culture environment, and in the incubator unit, photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic A control unit which controls to discharge the culture medium belonging to at least two groups of the organotrophic bacteria group according to a preset mixing ratio, and the mixer unit which mixes and stores the culture solution belonging to at least two groups discharged under the control of the control unit. It may include.

Description

Microbial culture and mixing device {MICROORGANISM MULTIPLYING AND MIXING APPARATUS}

The present invention relates to a microorganism cultivation and mixing apparatus that can provide a mixed microorganism cultured by mixing various microorganisms.

As is well known, various microorganisms such as photosynthetic bacteria, lactic acid bacteria, aerobic organic nutrients (bacillus), cyanobacteria, yongin bacteria, yeasts, etc. are used in agriculture, animal husbandry, fishing, and environmental fields.

When these microorganisms are used in agriculture, nitrogen is fixed in the soil and supplied to crops, which can be expected to reduce chemical fertilizers, and by removing toxic putrescine, cadaverine, and hydrogen sulfide from the soil, Can be removed.

In addition, it is possible to control pathogens by controlling various germs when disinfecting seeds, and when used in vegetables and fruit trees, actinomycetes increase, and by using the enzyme actinella chitanase, Fusilli bacteria, which are the main cause of disturbance, can be lysed. This can prevent serial disturbances and improve the coloring and storage of fruit trees.

On the other hand, the biochemical oxygen demand (BOD) can be lowered when used in manure treatment and wastewater treatment plants, and it can be used as a supplementary feed for animals or negatively administered to animals, thereby suppressing the growth of pathogens in the intestine, improving digestibility, and manure itself. Various effects such as reduction and mineral supply can be expected.

The use of microorganisms in fisheries can purify the water and remove suspended lakes and suspended solids.

As described above, various microorganisms are used throughout the daily life, and the microbial culture apparatus is commercialized in order to use a larger amount.

However, when cultivating a single microorganism, other microorganisms cannot be cultured until the microorganism is cultured, and since only a single microorganism is used or only microorganisms belonging to the same group are mixed and used, If you want to use, there is a problem in that after culturing a single microorganism or the same group of microorganisms currently being cultured, another microorganism or another group of microorganisms must be newly cultured and used.

The present invention is to provide a microbial culture and mixing apparatus that can culture the photosynthetic bacteria and cyanobacteria groups, lactic acid bacteria and yeast groups, aerobic organotrophic bacteria groups, respectively, and mix and provide microorganisms belonging to at least two groups.

In addition, the present invention is to provide a microbial culture and mixing apparatus that can be provided by mixing the microorganisms belonging to each group according to a predetermined culture environment, respectively, according to a predetermined culture ratio.

The objects of the embodiments of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description .

According to an embodiment of the present invention, an incubator unit having a culture chamber partitioned to culture photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group, the photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, Controlling the incubator unit to incubate each microorganism belonging to the aerobic organotrophic group according to a predetermined culture environment, wherein at least two of the photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, and aerobic organotrophic group Microorganism culture including a control unit for controlling to discharge the culture medium belonging to the two groups according to a preset mixing ratio, and a mixer unit for mixing and storing the culture solution belonging to the at least two discharged groups under the control of the control unit And a mixing device may be provided.

In the present invention, microorganisms belonging to photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group are cultured according to a predetermined culture environment, respectively, photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organic nutrition By discharging, storing and mixing at least two microorganisms in the bacterial group according to a predetermined mixing ratio, the mixed microorganisms can be cultured in large quantities, and the mixed microorganisms can be easily used in various applications.

1 is a block diagram of a microbial culture and mixing apparatus according to an embodiment of the present invention,
Figure 2 illustrates a microbial culture and mixing device according to an embodiment of the present invention,
Figure 3 is a flow chart showing a microbial culture and mixing process according to another embodiment of the present invention.

Advantages and features of the embodiments of the present invention, and methods of achieving them will be apparent with reference to the embodiments described below in detail with the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. To fully disclose the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims. Like reference numerals refer to like elements throughout.

In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. The following terms are defined in consideration of the functions in the embodiments of the present invention, which may vary depending on the intention of the user, the intention or the custom of the operator. Therefore, the definition should be based on the contents throughout this specification.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a block diagram of a microbial culture and mixing apparatus according to an embodiment of the present invention.

Referring to FIG. 1, the microbial culture and mixing apparatus according to an embodiment of the present invention may include an incubator unit 100, a control unit 200, a mixer unit 300, and the like.

The incubator unit 100 includes a plurality of incubators having a culture chamber partitioned to culture photosynthetic bacteria and cyanobacteria groups, lactic acid bacteria and yeast groups, aerobic organotrophic bacteria groups, and the incubator unit 100 includes photosynthetic bacteria and cyanobacteria. The bacteria culture unit 110, lactic acid bacteria and yeast culture unit 120, aerobic organic nutrient bacteria culture unit 130 may be included.

Here, the photosynthetic bacteria and cyanobacteria culture unit 110 has a first culture chamber insulated from other adjacent culture chambers, and provides light for photosynthesis in the first culture chamber according to the first culture environment. The first culture in the culture chamber is maintained at the first culture temperature, and the first microorganism of the group of photosynthetic bacteria and cyanobacteria can be cultured in a manner in which the first culture is circulated. Such photosynthetic bacteria and cyanobacteria groups can include, for example, Rodosudomonas Capsulata, Rhodomicrobium, Rodosudomonas Sphaeroides, Rhodobacter Capsulata, and the like.

The lactic acid bacteria and yeast culture unit 120 has a second culture chamber that is insulated from other adjacent culture chambers, and maintains the second culture liquid of the second culture chamber at the second culture temperature according to the second culture environment, and circulates the second culture liquid. The second microorganism of the lactic acid bacteria and yeast group can be cultured in a manner. Such lactic acid and yeast groups are described, for example, in Lactobacillus delbrukil subsp. lactis, Lactobacillus delbrukil subsp. delbrukil, Bifidobacterium bifidum bulgaricas, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus confusa, Saccharomyces Clipsoideas, Saccharomyces cerevisiae, Sacaromyses Sake and the like.

The aerobic organotrophic bacterium culture unit 130 has a third culture chamber that is insulated from other adjacent culture chambers, maintains the third culture solution of the third culture chamber at the third culture temperature according to the third culture environment, and circulates the third culture solution. The third microorganism of the group of aerobic organotrophic bacteria can be cultured in such a manner. Such aerobic organobacterial group may include, for example, Bacillus subtilis, Bacillus pumilus, Bacillus megaterium, Bacillus thuringiensis, Bacillus laterosporus, Bacillus licheniformis, and the like.

The control unit 200 controls the cultivation of each microorganism belonging to the photosynthetic bacteria and cyanobacteria group, the lactic acid bacteria and the yeast group, the aerobic organotrophic bacteria group, and the discharge and mixing of the culture solution belonging to at least two groups. The incubator unit 100 is controlled to cultivate each microorganism belonging to the fungal group, the lactic acid bacteria and the yeast group, and the aerobic organotrophic bacteria group according to a predetermined culture environment, and the incubator unit 100 controls the photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and The culture medium belonging to at least two groups of the yeast group and the aerobic organotrophic bacteria group may be controlled to be discharged and mixed according to a preset mixing ratio.

For example, the predetermined culture environment may include a culture temperature, a culture time, a culture unit operating state, and the like.

Here, the control unit 200 may include a culture environment display unit 210, an input unit 220, a control unit 230, and the like.

The culture environment display unit 210 may display a first culture environment, a second culture environment, and a third culture environment including a culture temperature, a culture time, and an operating state of each culture unit, respectively.

Input unit 220 is an operation signal for culturing each culture unit is input, a mixed signal for mixing each microorganism belonging to at least two groups is input, this operation signal or mixed signal is output to the controller 230 to be described later Can be.

The controller 230 maintains the first culture environment, the second culture environment, and the third culture environment for each culture unit according to the operation signal provided from the input unit 220, and controls the mixed signal provided from the input unit 220. Accordingly, the culture medium belonging to at least two groups may be controlled to be discharged according to a preset mixing ratio.

The mixer unit 300 stores and mixes a culture solution belonging to at least two groups discharged under the control of the control unit 200, and a detailed configuration thereof will be described later.

The mixer unit 300 may mix and store microorganisms belonging to at least two groups among photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, and aerobic organotrophic bacteria group. In this case, the activity of the mixed microorganisms may be rapidly increased and the growth may also be rapidly increased.

For example, mixed cultures of rodosudomonas capsulat in photosynthetic bacteria and Bacillus megaterium in aerobic organobacterials measure nitrogen fixation properties, which show significantly enhanced nitrogen fixation properties than when cultured with photosynthetic bacteria alone. This is because photosynthetic bacteria coexist with both aerobic organotrophic bacteria, which are non-nitrogen-fixing bacteria, and nitrogen is fixed.

In addition, Lactobacillus in photosynthetic bacteria and lactic acid bacteria can significantly promote the nitrogen fixation properties of photosynthetic bacteria when mixed than cultured alone.

In the case of yeast, coexisting with photosynthetic bacteria, aerobic organic nutrients, and lactic acid bacteria can serve as a nutrient for each microorganism, thus providing a better growth culture environment, and at the final stage of fermentation, it will play an active role in fermentation. It has a function of synthesizing and reducing amino acids, vitamins, nucleic acids, minerals, hormones, fatty acids, etc., and may also play a role of a synthesizer synthesizing eight essential amino acids.

Therefore, microorganisms belonging to the photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group were cultured according to the predetermined culture environment, respectively, photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organic nutrition bacteria group By discharging, storing and mixing at least two microorganisms according to a predetermined mixing ratio, the mixed microorganisms can be cultured in large quantities, and the mixed microorganisms can be easily used in various applications.

Next, microorganisms belonging to photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group were cultured according to a predetermined culture environment, respectively, and photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organic nutrition bacteria An example of a microbial culture and mixing apparatus for discharging, storing, and providing at least two microorganisms in a group according to a predetermined mixing ratio will be described.

2 is a diagram illustrating a microbial culture and mixing apparatus according to an embodiment of the present invention.

2, the microbial culture and mixing apparatus according to an embodiment of the present invention may include an incubator unit 100, a control unit 200, a mixer unit 300, and the like. Photosynthetic bacteria and cyanobacteria culture unit 110, lactic acid bacteria and yeast culture unit 120, aerobic organic nutrient bacteria culture unit 130, the control unit 200 is a culture environment display unit 210, input unit 220 , The controller 230, and the mixer unit 300 may include a plurality of open / close valves 310, a pumping motor 320, a mixed microorganism storage tank 330, and a connection pipe 340.

Here, photosynthetic bacteria and cyanobacteria culture unit 110 is the first supply pipe 111, the light source 112, the first heater 113, the first temperature sensor 114, the first circulator 115, the first It may include an oxygen supplier 116, a first outlet 117, a first flow meter 118, and the like. In this case, a first heat insulating member may be provided inside the outer wall C of the first culture chamber to insulate the other culture chamber adjacent to the outside.

The first supply pipe 111 extends from the upper side of the first culture chamber to the lower side to supply the first culture medium of the group of photosynthetic bacteria and cyanobacteria to the first culture chamber, and the light source 112 is provided on the outer upper side and the side surface of the first culture chamber. It may be provided to provide light for photosynthesis inside the first culture chamber. Since the light emitting part of the light source 112 is a part in direct contact with light, photosynthetic bacteria and microorganisms of the microorganism group may be killed, and a washing machine may be provided to wash such dead bodies, and the dead washed through the washing machine. A dead body discharge port for discharging the sieve may be provided together.

The first heater 113 is provided with a heating wire inside the outer wall (C) of the first culture chamber to maintain the first culture temperature, the first temperature sensor 114 is provided inside the first culture chamber, the culture solution of the first culture chamber The temperature can be measured and provided.

The first circulator 115 may include an underwater pump, an underwater mixer, and the like, and may circulate the first culture solution convectively, and the first oxygen supplier 116 may include a bubble generator, and the like. When culturing cyanobacteria in the bacteria group, oxygen may be supplied to the first culture solution to provide an aerobic culture environment.

In the photosynthetic bacteria and cyanobacteria culture unit 110, a first discharge valve 310a is selectively opened in the lower portion under the control of a control unit, so that the cultured first culture solution is discharged according to a preset mixing ratio 117. ) May be provided, and a first flow meter 118 may be provided to indicate the amount of discharge of the first culture solution.

The lactic acid bacteria and yeast culture unit 120 includes a second supply pipe 121, a second heater 122, a second temperature sensor 123, a second circulator 124, a second outlet 125, and a second flow meter 126. ) May be included. At this time, the inside of the outer wall (C) of the second culture chamber may be provided with a second heat insulating member to insulate the other adjacent culture chamber and the outside.

The second supply pipe 121 extends from the top of the second culture chamber to the bottom thereof to supply the second culture medium of the lactic acid bacteria and the yeast group to the second culture chamber, and the second heater 122 is the outer wall C of the second culture chamber. A heating wire is provided inside to maintain the second culture temperature, and the second temperature sensor 123 may be provided inside the second culture chamber to measure and provide a culture temperature of the second culture chamber.

The second circulator 124 includes, for example, an underwater pump, an underwater mixer, and the like, and can circulate the second culture solution in the second culture chamber in a convection manner.

The lactic acid bacteria and yeast culture unit 120 may be provided with a second discharge port 125 through which the second discharge valve 310b is selectively opened under the control of the control unit, and the cultured second culture solution is discharged according to a preset mixing ratio. A second flow meter 126 may be provided to indicate the amount of discharge of the second culture solution.

The aerobic organobacterial bacterial culture unit 130 includes a third supply pipe 131, a third heater 132, a third temperature sensor 133, a third circulator 134, a second oxygen supply 135, and a third outlet. 136, a third flow meter 137, and the like. In this case, a third heat insulating member may be provided inside the outer wall C of the third culture chamber to insulate the other culture chambers adjacent to the outside.

The third supply pipe 131 extends from the upper portion of the third culture chamber to the lower portion to supply the third culture solution of the group of aerobic organotrophic bacteria to the third culture chamber, and the third heater 132 is an outer wall C of the third culture chamber. The inside of the heating wire may be provided to maintain the third culture temperature, and the third temperature sensor 133 may be provided inside the third culture chamber to measure and provide a culture medium temperature of the third culture chamber.

The third circulator 134 may include an underwater pump, an underwater mixer, and the like, and may circulate the third culture solution in a convection manner, and the second oxygen supply 135 may include a bubble generator and the like. Oxygen may be supplied to the third culture for culturing the group to provide an aerobic culture environment.

The aerobic organotrophic bacterium culture unit 130 has a third discharge valve 310c selected and opened under the control of the control unit at the lower portion thereof so that the cultured third culture solution is discharged according to a preset mixing ratio. It may be provided, and may be provided with a third flow meter 137 for displaying the amount of the third culture solution is discharged.

In addition, the controller unit 200 displays malfunctions of the controller 230 and the culture environment of the photosynthetic bacteria and cyanobacteria culture unit 110, the lactic acid bacteria and yeast culture unit 120, and the aerobic organotrophic bacteria culture unit 130. The culture environment display unit 210 is provided, and the input unit 220 is provided with a plurality of buttons (switches) for culturing the microorganisms to be cultured in each culture unit or mixing the microorganisms. It may be provided inside the controller unit 200.

When a button (switch) for culturing microorganisms is pressed through such a configuration, a culture signal for providing a predetermined culture environment for culturing the microorganism is input to the controller 230, and accordingly, the controller 230 controls each culture. Each culture environment of the department can be adjusted.

For example, when a button (switch) for microbial cultivation of photosynthetic bacteria and cyanobacteria group is pressed, the controller 230 cultures photosynthetic bacteria and cyanobacteria as a first culture environment for microbial culture of photosynthetic bacteria and southern microorganism group. The unit 110 may be adjusted. When the first culture solution including photosynthetic bacteria or cyanobacteria is supplied to the first culture chamber, electricity is supplied to the light source 112 to emit light, and then the appropriate culture temperature is set as the first culture environment. Is 30 ° C., the first heater 113 is selectively driven to maintain an appropriate culture temperature of 30 ° C. according to the internal temperature measured by the first temperature sensor 114, and the first circulator 115 is driven to 1 culture can be circulated. In particular, when culturing Bacillus bacteria, the first oxygen supplier 116 may be operated to supply oxygen to the Bacillus culture medium.

When a button (switch) for culturing is pressed during the microbial culture of the lactic acid bacteria and yeast group, the controller 230 may control the lactic acid bacteria and the yeast culture unit 120 as a second culture environment for microbial culture of the lactic acid bacteria and the yeast group. When the second culture solution containing lactic acid bacteria or yeast is supplied to the second culture chamber, when the proper culture temperature set as the second culture environment is 35 ° C., the proper culture temperature is determined according to the internal temperature measured by the second temperature sensor 123. The second heater 122 may be selectively driven to maintain the phosphorus at 35 ° C., and the second circulator 124 may be driven to circulate the second culture solution.

When a button (switch) for culturing is pressed during microbial culture of an aerobic organic nutrient bacterium group, the controller 230 is an aerobic organic nutrient bacterial culture unit 130 as a third culture environment for culturing the aerobic organic nutrient bacterium group. When the third culture solution containing aerobic organotrophic bacteria is supplied to the third culture chamber, when the proper culture temperature set to the third culture environment is 40 ° C., the internal temperature measured by the third temperature sensor 133 is measured. Accordingly, the third heater 132 is selectively driven to maintain an appropriate culture temperature of 40 ° C., and the second circulator 134 is driven to circulate the first culture medium, thereby providing an aerobic environment for culturing aerobic organic nutrients. In order to operate the second oxygen supply 135 to supply oxygen to the aerobic microorganism bacteria culture medium.

Meanwhile, the mixer unit 300 may include a plurality of open / close valves 310, a pumping motor 320, a mixed microorganism storage tank 330, and a connection pipe 340. Here, the plurality of open / close valves 310 may include a first open / close valve 310a provided under the first culture chamber, a second open / close valve 310b provided under the second culture chamber, and a third provided under the third culture chamber. The on / off valve 310c may be included.

The plurality of open / close valves 310 may include a first open / close valve 310a provided under the first culture chamber, a second open / close valve 310b provided under the second culture chamber, and a third open / close valve provided under the third culture chamber ( 310c), each of which may be selectively opened at the time of discharge of the culture medium belonging to at least two groups.

The pumping motor 320 simultaneously discharges the culture solution belonging to at least two groups according to the selective opening of the plurality of open / close valves at the same time according to the preset mixing ratio. After being transferred to 330, it may be mixed. Here, the mixed microorganism storage tank 330 may be provided with a discharge valve 331 for discharging the mixed microorganism in the lower portion.

The plurality of opening and closing valve 310, the pumping motor 320 and the mixed microorganism storage tank 330 as described above may be connected to each other through a connection pipe 340, the culture medium is transported and mixed.

For example, when a button (switch) for mixing microorganisms belonging to at least two groups among photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, and aerobic organotrophic bacteria group is pressed, the controller 230 controls the plurality of open / close valves. By opening and closing valves respectively installed in the culture unit in which the corresponding microorganisms are cultured, and simultaneously driving the pump motor 320, the microorganisms are transferred through the connection pipe 340 to mix the microorganisms in the storage tank 330. Can be mixed. Such on-off valves may be manually closed or automatically closed according to a preset mixing ratio.

Meanwhile, the first heater 113, the second heater 122, and the third heater 132 as described above prevent direct damage of microorganisms due to heat, and risk of leakage of water due to moisture generated when installed in each culture chamber. It may be provided inside the outer wall (C) of each culture chamber to prevent the. In addition, in the summer and autumn, the temperature of microorganisms can be maintained at an appropriate temperature. However, in winter and early spring, the temperature of the microorganism can not be maintained due to a decrease in the temperature of the microorganisms. In order to provide a first heat insulating member, a second heat insulating member, and a third heat insulating member in the outer wall C of each culture chamber, the influence of the temperature change can be minimized.

And, photosynthetic bacteria and cyanobacteria culture unit 110, lactic acid bacteria and yeast culture unit 120, aerobic organic nutrient bacteria culture unit 130 as described above due to the discharge and internal cleaning to the mixer unit 300, respectively A dead body discharge port for discharging the killed microorganism dead body to the outside may be further provided.

Therefore, microorganisms belonging to the photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group were cultured according to the predetermined culture environment, respectively, photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organic nutrition bacteria group By discharging, storing and mixing at least two microorganisms according to a predetermined mixing ratio, the mixed microorganisms can be cultured in large quantities, and the mixed microorganisms can be easily used in various applications.

Next, microorganisms belonging to the photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group in the microorganism culture and mixing apparatus having the above-described configuration are respectively cultured according to the predetermined culture environment, and the predetermined mixing The process of discharging, storing and mixing the culture medium in which the microorganisms are cultured according to the ratio will be described.

3 is a flow chart showing a microbial culture and mixing process according to another embodiment of the present invention.

Referring to FIG. 3, the control unit 200 checks whether the microorganism is cultured by checking that the culture signal for culturing the microorganism is input through the input unit 220 (S302). The input unit 220 is provided with a culture button (culture switch) corresponding to photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group. Of course, according to the operation of the culture button (culture switch) can be classified and cultured photosynthetic bacteria and cyanobacteria, lactic acid bacteria and yeast can also be cultured separately.

As a result of the check in step S302, when a culture signal for culturing microorganisms is input, the control unit 200 controls to supply a culture solution for culturing microorganisms to the incubator unit 100, and accordingly, the incubator unit 100. In the supply of the culture medium containing the microorganism for the culture of the microorganism (S304).

In addition, the control unit 200 controls to adjust to a predetermined culture environment corresponding to the supplied culture solution, thereby adjusting the culture environment of each culture chamber provided in the incubator unit 100 (S306).

For example, after the light source 112 emits light to provide a first culture environment for culturing photosynthetic bacteria and microorganisms of the group of microorganisms, the appropriate culture temperature is determined according to the internal temperature measured by the first temperature sensor 114. The first heater 113 may be selectively driven to maintain phosphorus 30 ° C., and the first culture medium may be circulated convectionally through the first circulator 115. In particular, during the culture of Bacillus microbes, oxygen may be supplied to the Bacillus culture medium through the first oxygen supplier 116.

And, the second heater 122 to maintain a suitable culture temperature of 35 ℃ according to the internal temperature measured by the second temperature sensor 123 to provide a second culture environment for culturing the microorganisms of lactic acid bacteria and yeast group May be selectively driven, and the second culture may be circulated convectionally through the second circulator 124.

In addition, the third heater (3) to maintain an appropriate culture temperature of 40 ° C according to the internal temperature measured by the third temperature sensor 133 in order to provide a third culture environment for cultivating microorganisms of the group of aerobic organotrophic bacteria. 132 may be selectively driven, and the first culture solution may be convectively circulated through the second circulator 134, and oxygen may be supplied to the aerobic organobacterial bacterial culture medium through the second oxygen supplier 135.

It is possible to culture the microorganism to be cultured according to the above-described process and culture environment (S308).

On the other hand, the control unit 200 checks whether the mixed microorganisms in a manner to check that the mixed signal for mixing the microorganisms through the input unit 220 (S310). The input unit 220 is provided with a mixing button (mixing switch) for mixing the microorganisms belonging to at least two groups of photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group according to a preset mixing ratio have. Of course, the mixing ratio may be variously set as necessary.

As a result of the check in step S310, when a mixing signal for mixing microorganisms is input, the control unit 200 controls the mixer unit 300 to open the plurality of on / off valves 310 provided in the mixer unit 300. At the same time by selectively opening and driving the pumping motor 320, the culture medium is discharged from at least two groups of photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organic nutrient bacteria group (S312).

Subsequently, the discharged culture solution is transferred through the connection pipe 340 and stored and mixed in the mixed microorganism storage tank 330 (S314).

In livestock farms using mixed mixed microorganisms as described above, it is possible to remove odors in the barn and promote livestock growth, improve meat quality, increase feed efficiency, reduce odor of manure, reduce flies, reduce fermentation and odor of manure, and BOD of manure. It can be used variously for the purpose of reduction.

By adjusting the mixing ratio of these mixed microorganisms, a large effect can be expected. The odors in the barn are mostly odors generated from livestock meals, and the components of the odor gas in pig farms include acetic acid, propionic acid, butyric acid, isolaxic acid, gil acetic acid, Isogyl acetic acid and the like, and photosynthetic bacteria can remove not only such gases but also putrescine, cadaverine, hydrogen sulfide and the like, which are highly toxic secondary amines.

In addition, the aerobic organotrophic bacteria can directly decompose the manure at the bottom of the barn to quickly decompose the manure, suppress the generation of decay bacteria to strongly suppress the generation of gas, it is possible to easily remove the smell of the barn.

On the other hand, yeast synthesizes various substances such as amino acids, vitamins, nucleic acids, minerals, hormones and fatty acids, and reduces these useful substances for use by other microorganisms at the end of the growth cycle, so that yeasts are also photosynthetic bacteria and aerobic organic nutrients. Overspreading can promote the growth of photosynthetic bacteria and aerobic organic nutrients.

Accordingly, the mixing ratio of the mixed microorganisms to remove the odor in the barn is the maximum ratio of photosynthetic bacteria and by adding aerobic organotrophic bacteria, not only to promote the activity of photosynthetic bacteria, but also to suppress the generation of gas due to decayed bacteria In addition, by increasing the activity of the two bacteria with yeast can be a great effect in removing the odor.

For example, if the standard of mixed spraying is 20 l / 100 pyeong, and the unit of the ratio is 1 l, the mixing ratio for removing odor in the barn is "photosynthetic bacteria: aerobic organic nutrients: yeast = 10: 8: 2". It is preferable to set to.

Recently, live microorganisms are administered to livestock directly or through other media to promote livestock growth, improve meat quality, increase feed efficiency, and reduce flies due to reduced manure odor. Probiotics) is a mixture of photosynthetic bacteria, lactic acid bacteria, and yeasts cultured using the microorganism culture and mixing device as described above to improve the meat quality, increase the feed efficiency, reduce the smell of manure and reduce the fly when administered to livestock You can get it.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be readily apparent that such substitutions, modifications, and alterations are possible.

100: incubator unit 110: photosynthetic bacteria and cyanobacteria culture unit
120: lactic acid bacteria and yeast culture unit 130: aerobic organic nutrition bacteria culture unit
200: control unit 210: culture environment display unit
220: input unit 230:
300: mixer unit 310: a plurality of open and close valves
320: pumping motor 330: mixed microorganism storage tank
340 connector

Claims (8)

delete delete delete delete An incubator unit having a culture chamber partitioned to culture photosynthetic bacteria and cyanobacteria groups, lactic acid bacteria and yeast groups, aerobic organotrophic bacteria groups,
Controlling the incubator unit to incubate each microorganism belonging to the photosynthetic bacteria and cyanobacteria group, lactic acid bacteria and yeast group, aerobic organotrophic bacteria group according to a predetermined culture environment, and in the incubator unit, the photosynthetic bacteria and cyanobacteria group, A control unit which controls to discharge the culture solution belonging to at least two groups of the lactic acid bacteria, the yeast group, and the aerobic organotrophic bacteria group according to a preset mixing ratio,
A mixer unit for mixing and storing the culture solution belonging to the at least two discharged groups under the control of the control unit,
The incubator unit,
It has a 1st culture chamber insulated from another adjacent culture chamber, and provides the light for photosynthesis operation | movement inside the said 1st culture chamber according to a 1st culture environment, and maintains the 1st culture liquid of the said 1st culture chamber at 1st culture temperature. A photosynthetic bacteria and cyanobacteria culture unit for culturing the first microorganisms of the photosynthetic bacteria and cyanobacteria group by supplying oxygen to the first culture medium and circulating the first culture medium;
The lactic acid bacteria and yeast group having a second culture chamber insulated from other adjacent culture chambers, maintaining the second culture liquid of the second culture chamber at the second culture temperature according to the second culture environment, and circulating the second culture liquid. Lactic acid bacteria and yeast culture unit for culturing the second microorganism,
A third culture chamber insulated from other adjacent culture chambers, the third culture solution of the third culture chamber is maintained at a third culture temperature according to a third culture environment, oxygen is supplied to the third culture solution, and the third culture solution Including aerobic organic nutrient bacteria culture unit for culturing the third microorganism of the aerobic organic nutrient bacteria group in a circulating manner,
Wherein the control unit comprises:
A culture environment display unit for respectively displaying the first culture environment, the second culture environment, and the third culture environment including a culture temperature, a culture time, and an operating state of each culture section;
An input unit for inputting an operation signal for culturing each culture unit, and a mixed signal for mixing each microorganism belonging to the at least two groups;
The first culture environment, the second culture environment and the third culture environment are respectively maintained for each of the culture units according to the operation signal, and the culture solution belonging to the at least two groups is respectively discharged according to the mixed signal. It includes a control unit for controlling to mix according to the set mixing ratio,
The mixer unit,
A plurality of on / off valves that are selectively opened at the time of discharging the culture solution belonging to the at least two groups,
A pumping motor for simultaneously discharging a culture solution belonging to the at least two groups corresponding to the selective opening of the plurality of on / off valves at the same time according to the preset mixing ratio;
A mixed microorganism storage tank configured to simultaneously store the mixed culture solution discharged and mixed according to the preset mixing ratio;
A connecting pipe connecting the plurality of on / off valves, a pump motor, and a mixed microorganism storage tank,
The photosynthetic bacteria and cyanobacteria culture unit,
A first supply pipe extending from an upper portion of the first culture chamber to a lower portion to supply the first culture solution of the photosynthetic bacteria and cyanobacteria group to the first culture chamber;
A first heat insulating member provided inside the outer wall of the first culture chamber to insulate the adjacent other culture chambers and the outside;
A light source provided at an outer upper side and a side portion of the first culture chamber, the light source having the light provided inside the first culture chamber, and washing the dead body generated during the culture of the photosynthetic bacteria and cyanobacteria group;
Killer body outlet for discharging the killing body washed through the washing machine,
A first heater for maintaining the first culture temperature through a heating wire provided inside the outer wall of the first culture chamber,
A first temperature sensor for measuring and providing a culture temperature of the first culture chamber;
A first circulator for circulating the first culture in a convection manner,
A first oxygen supplier for supplying oxygen to the first culture medium for culturing cyanobacteria among the photosynthetic bacteria and cyanobacteria group;
A first discharge port through which the first culture medium is selected and opened under the control of the control unit and the culture medium is discharged according to the preset mixing ratio.
Microbial culture and mixing device comprising a.
The method of claim 5, wherein
The lactic acid bacteria and yeast culture unit,
A second supply pipe extending from an upper portion of the second culture chamber to a lower portion to supply the second culture solution of the lactic acid bacteria and the yeast group to the second culture chamber;
A second heat insulating member provided inside the outer wall of the second culture chamber to insulate the adjacent other culture chambers and the outside;
A second heater for maintaining the second culture temperature through a heating wire provided inside the outer wall of the second culture chamber;
A second temperature sensor for measuring and providing a culture temperature of the second culture chamber;
A second circulator for circulating the second culture liquid in a convection manner,
A second discharge port through which a second discharge valve is selectively opened according to the control of the control unit so that the culture medium is discharged according to the preset mixing ratio;
Microbial culture and mixing device comprising a.
The method of claim 5, wherein
The aerobic organotrophic bacteria culture unit,
A third supply pipe extending from an upper portion of the third culture chamber to a lower portion to supply a third culture solution of the aerobic organotrophic bacterial group to the third culture chamber;
A third heat insulating member provided inside the outer wall of the first culture chamber to insulate the adjacent other culture chambers and the outside;
A third heater for maintaining the third culture temperature through a heating wire provided inside the outer wall of the third culture chamber;
A third temperature sensor for measuring and providing a culture temperature of the third culture chamber;
A third circulator for circulating the third culture solution in a convection manner,
A second oxygen supplier for supplying oxygen to the third culture solution for culturing the aerobic organotrophic bacteria group;
A third discharge port through which a third discharge valve is selectively opened according to the control of the control unit so that the culture medium is discharged according to the preset mixing ratio
Microbial culture and mixing device comprising a.
8. The method according to any one of claims 5 to 7,
The incubator unit is characterized in that it comprises a flow meter for displaying the discharge amount when the discharge of the culture medium belonging to the at least two groups, respectively, in the photosynthetic bacteria and cyanobacteria culture section, lactic acid bacteria and yeast culture section and aerobic organotrophic bacteria culture section Microbial culture and mixing device.

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