KR101211579B1 - Making method of mixed feed for odor control from livestock - Google Patents

Abstract

The present invention is characterized by analyzing the fermentation products of each sweet rice feed, the method of producing a blended feed adjusted the mixing ratio of sweet rice feed and a method of predicting the degree of odor occurrence when fed to the breeding animal based on the fermentation products of the feed.

The production method according to the present invention can adjust the selection, exclusion and blending amount of the short feed in the preparation of the compound feed to reduce the amount of odor generated in the manure of breeding animals, it can be very effectively applied to the development of environment-friendly pig feed.

Single feed, compound feed, anaerobic fermentation, odor removal

Description

Making method of mixed feed for odor control in breeding animals {Making method of mixed feed for odor control from livestock}

The present invention is characterized by analyzing the fermentation products of each sweet rice feed, the method of producing a blended feed adjusted the mixing ratio of sweet rice feed and a method of predicting the degree of odor occurrence when fed to the breeding animal based on the fermentation products of the feed.

In livestock, when lactic acid fermentation does not occur in the large intestine, pathogenic microorganisms multiply and toxins are produced. Increasing the concentration of toxins produced by pathogenic microorganisms decreases the activity of the intestinal mucosa, and in severe cases, the intestinal mucosa may be necrotic and cause disease.

In order to lower the vitality of pathogenic microorganisms and increase the vitality of lactic acid bacteria, it is necessary to provide a substrate that is well utilized by lactic acid bacteria. The higher the vitality of lactic acid bacteria, the lower volatile fatty acids such as acetic acid, propionic acid, and butyric acid, which are used as energy sources for intestinal epithelial cells, are increased. Compounds such as indole, skatole, phenol and p-cresol increase. For this reason, research is being conducted to provide substrates required by lactic acid bacteria for improving livestock productivity and eco-friendly livestock.

Korean Registered Patent No. 381,726 discloses a feed additive for aquaculture fish using a citrus fermentation broth, and Korean Laid-Open Patent Publication No. 10-1999-0046622 discloses a method for producing livestock feed using fig leaves and berries. have. In addition, Korean Patent No. 336,411 discloses a method for producing a feed by anaerobic lactic fermentation at a high temperature of 40 ~ 70 ℃.

However, the prior art had a limitation in suppressing odor generated from pathogenic microorganisms and manure of livestock, and techniques for suppressing odor by analyzing fermented ingredients of sweet rice are not known until now.

Thus, the present inventors analyzed and fermented products produced by fermenting each of the single feed constituting the compound feed as a result of research and efforts to produce a compound feed to suppress the generation of odor, and based on this, by adjusting the mixing ratio of the single feed is malodor The present invention has been completed by discovering that the occurrence can be minimized.

Therefore, an object of the present invention is to provide a method for preparing a compound feed in which the compounding ratio of the sweet feed is controlled by analyzing fermentation products of the single feed.

The present invention,

In the method of manufacturing a compound feed by mixing two or more single feed,

Anaerobic fermentation of sweet rice feed;

Measuring a fermentation product produced by the fermentation;

Comparing and confirming the concentrations of fermented products between the sweet rice feeds; And

Mixing and adjusting the blending ratio of each sweet feed according to the concentration of the fermentation product

Characterized in that the method for producing a compound feed to suppress the generation of odor of a breeding animal, including.

In another aspect, the present invention is characterized in that the anaerobic fermentation of the feed, measuring the fermentation products produced by the fermentation, and predicts the degree of odor occurrence when feeding the prepared feed based on the measured value of the fermentation products .

The production method according to the present invention can adjust the selection, exclusion and blending amount of the short feed in the preparation of the compound feed to reduce the amount of odor generated in the manure of breeding animals, it can be very effectively applied to the development of environment-friendly pig feed.

The present invention is anaerobic fermentation of sweet rice feed, measuring the fermentation products produced by the fermentation, comparing and confirming the concentration of fermentation products between each sweet feed, and the combination of each sweet feed according to the concentration of the fermentation products Characterized by a method for producing a compound feed to suppress the generation of odors of a breeding animal, including the step of adjusting the ratio.

As feed used for breeding pigs and the like, a compound feed containing a single feed is generally used. In the present invention, it is possible to minimize the amount of odor generated in the breeding animals by adjusting the selection and blending ratio of the short feed. The breeding animal is not limited in kind, but preferably pigs are most suitable.

Anaerobic fermentation of the sweet rice used first to produce a fermentation product. The sweet rice feed is not limited if the feed can be included in the compound feed, but preferably, barley, lupine seed, wheat flour, rice barley, corn, wheat, protein peel, rice bran, bran, beet pulp, tapioca, soybean meal Can be selected from wheat gluten, coconut, corn germ, spirits, rapeseed, corn gluten, palm oil, horse mackerel, fish meal, jade germ meal, feather meal, meat bone meal, sardine meal, blood meal and plasma protein. .

The anaerobic fermentation is preferably made by mixing pig flour and microbial medium, the anaerobic fermentation is preferably performed for 20 to 80 hours at 20 ~ 50 ℃.

Thereafter, the concentration of the fermentation product produced by the anaerobic fermentation is measured. The fermentation products are acidified in the environment of the colon and lower volatile fatty acids composed of acetic acid, propionic acid, butyric acid, which inhibit the growth of harmful microorganisms, and act as toxins in the body, thereby lowering the productivity of breeding animals and discharging them into the body. Phenol, p-cresol, indole, and skatole, which act as a scavenger, are preferably selected from the above compounds and used as an indicator of concentration measurement.

Thereafter, check and compare the concentrations of fermented products measured between each sweet feed. There is a difference in the concentration of the fermentation product according to the constituent material for each sweet rice feed, it is possible to predict the effect of odor generation.

On the basis of the concentration of the fermentation product compared and confirmed above, the blending ratio of each sweet feed is adjusted according to the concentration of the fermentation product. That is, in the case of a short feed having a high concentration of a specific fermentation product that affects the occurrence of odor, the odor generation can be reduced without loss of nutrients by combining with a short feed having a low concentration of the specific fermentation product. When blending the analysis of the concentration of fermentation products for each sweet rice can be used to control the blending ratio.

In another aspect, the present invention is characterized in that the anaerobic fermentation of the feed, measuring the fermentation products produced by the fermentation, and predicts the degree of odor occurrence when feeding the prepared feed based on the measured value of the fermentation products .

 The breeding animal is not limited in kind, but preferably pigs are most suitable, and the anaerobic fermentation is preferably made by mixing pig meal and a microbial medium, and the anaerobic fermentation is performed at 20 to 50 ° C. for 20 to 80 hours. It is desirable to proceed for a while.

In addition, the concentration of the fermentation product produced by the anaerobic fermentation is measured, and the level of odor occurrence during feeding to the breeding animal using low volatile fatty acids and fermentation products such as phenol, p-cresol, indole and scatol as indicators. It can be predicted.

Hereinafter, the present invention will be described in more detail based on the following examples, but the present invention is not limited to the following examples.

Example 1 Determination of Concentration of Fermented Products by Single Feed

Barley, Lupine Seed, Wheat Flour, Rice Barley, Corn, Wheat, Protein Peel, Rice Bran, Bran, Beet Pulp, Tapioca, Soybean Meal, Wheat Gluten, Coconut, Corn Germ, Liquor, Chameleon, Corn Gluten, Palm Oil, Homa Twenty-seven kinds of sweet meal of gourd, fish meal, jade embryo powder, feather powder, meat bone meal, sardine powder, blood meal, and plasma protein were put in 20 mL bottles of 0.2 g each.

Since based on 1L of water, to prepare a microbial medium composition having a composition of Table 1.

Composition content Sodium bicarbonate (NaHCO ₃ ) 5g Sodium chloride (NaCl) 0.9 g Potassium phosphate (KH ₂ PO ₄ ) 0.45 g _{Potassium phosphate (K 2 HPO 4?} 3H 2 O) 0.45 g Magnesium chloride (MgCl ₂ ) 0.02 g _{Manganase sulfate (MnSO 4? 4H 2} O) 0.01 g Ammonium sulfate ((NH ₄ ) ₂ SO ₄ ) 0.9 g Cobalt chloride hexahydrate (CoCl ₂ ? 6H ₂ O) 0.01 g Calcium chloride (CaCl ₂ ? 2H ₂ O) 0.03 g Iron sulfate heptahydrate (FeSO ₄ ? 7H ₂ O) 0.01 g

Meanwhile, 100 g of pig meal and 1 L of the prepared microbial medium were mixed, and the mixture of the mixed pig meal and the microbial medium was put in a 10 mL bottle containing sweet rice feed, and anaerobic fermentation was performed at 38 ° C. for 48 hours.

Among the fermentation products produced by the fermentation, the concentrations of lower volatile fatty acids, phenol, p-cresol, indole and scatol, which consist of acetic acid, propionic acid and butyric acid, were measured, and the increase was based on the concentrations measured in general fermented pig meal. Or confirmed the degree of reduction, the results are shown in Table 2 below.

Sweet rice feed Fermentation product concentration in ppm Low volatility
fatty acid phenol p-cresol Indole Skatol Fermentation Powder (ppm) 1293 0.82 9.51 3.40 3.68 Shelter 3307 -0.37 25.98 -1.69 -0.89 Lupine seed 3385 -0.08 57.27 -0.88 1.38 flour 4002 0.01 28.06 -1.56 -1.33 Rice barley 3616 -0.47 27.17 -1.68 -1.17 corn 3322 -0.82 19.86 -1.91 -1.43 Wheat 3568 -0.43 34.70 -1.65 -0.51 Protein 2242 1.44 45.38 -1.26 -0.93 Rice bran 2349 4.09 28.76 -1.27 0.29 bran 2769 0.36 33.02 -0.55 1.15 Beet pulp 3197 -0.82 26.55 -1.66 -1.70 tapioca 3516 0.05 7.78 -1.83 -2.06 Soybean meal 2766 0.07 66.29 0.39 3.74 Wheat Gluten 3735 -0.19 87.09 9.84 15.89 Palm Foil 2585 1.69 13.76 -1.85 -1.87 Corn germ 2389 1.65 30.97 -1.63 -0.13 Berth 2029 1.21 31.92 -1.29 -0.92 Chaejongbak 2308 0.31 43.72 2.21 -2.00 Corn gluten 1211 0.52 30.34 -0.62 -0.83 Palm oil 1490 8.52 16.13 -1.31 -1.34 Homabak 1951 0.02 28.79 1.67 1.03 Fish meal 1814 0.21 52.20 2.11 1.67 Ox amber 2731 -0.82 20.25 -1.81 -1.95 Feather 501 0.43 28.00 0.79 -0.29 Meat bone meal 1181 0.29 35.52 -0.55 -0.05 Sardine 1535 -0.24 49.30 0.87 1.48 Blood powder 353 0.23 23.73 3.14 0.07 Plasma protein 3159 -0.10 246.96 70.13 27.96

As shown in Table 2, the concentrations of fermentation products causing malodors were different for each sweet rice feed, and on the basis of this, it was possible to predict the degree of malodor occurrence when feeding the sweet rice feed to pigs. Comparing the concentrations of fermented ingredients of the samples and combining them, it was possible to derive a blending ratio that minimizes the occurrence of odor while maintaining nutrients at a certain level.

Example 2 Preparation of Compound Feed

Comparing the fermentation product concentration of each sweet rice shown in Table 2 to prepare a blended feed in the blending ratio of Table 3 to minimize the occurrence of odor while maintaining nutrients to a certain level. Meanwhile, the feed of the comparative example was prepared based on the nutrient recommendation level required for pigs weighing 20 to 50 kg in the US specification standard (NRC, 1988), and the compounding ratio for each single feed is shown in Table 3 below.

Feed name Example 2 Comparative example corn 45.43 65.01 Beet pulp 3.00 - tapioca 17.72 - Soybean Scalp 5.94 - bran - 7.25 Soybean meal 18.10 12.01 Chaejongbak - 7.65 Fish meal 4.42 2.17 Lysine - 0.19 Limestone 0.28 1.03 molasses 2.00 2.00 Vitamin Mineral Additives 0.35 0.35 TCP 0.46 0.04 Salt 0.30 0.30 Soybean oil 2.00 2.00 sum 100.00 100.00 Nutrient content DE (kcal / kg) 3400 3400 protein(%) 16.00 16.00 calcium(%) 0.60 0.60 sign(%) 0.50 0.50 Lysine (%) 0.96 0.95 Methionine (%) 0.30 0.30 Threonine (%) 0.63 0.61

Test Example  : Determination of the concentration of odor generating components according to the formula feed

After feeding the feeds of Example 2 and Comparative Example for each of the five growing pigs for 28 days, the concentrations of phenol, p-cresol, indole and scartol generated in pig meal were measured and are shown in Table 4 below.

Odor Concentration Example 2 Comparative example Phenol (mg / kg) 0.38 0.82 p-cresol (mg / kg) 7.27 9.51 Indole (mg / kg) 2.80 3.40 Scatol (mg / kg) 3.33 3.68

As shown in Table 4, compared to the feed of the comparative example, it was confirmed that the concentration of the malodor generating component was greatly reduced in the blended feed according to the present invention. Therefore, the present invention can be applied to control the selection and the blending ratio of the single feed in order to minimize the generation of odor in the production of the mixed feed mixed with sweet rice feed.

Claims (7)

In the method of manufacturing a compound feed by mixing two or more single feed, Anaerobic fermentation of sweet rice feed; Measuring a fermentation product selected from among lower volatile fatty acids, phenol, p-cresol, indole and scaldol produced by the fermentation; Comparing and confirming the concentrations of fermented products between the sweet rice feeds; And Mixing and adjusting the blending ratio of each sweet feed according to the concentration of the fermentation product Method for producing a compound feed, characterized in that to suppress the generation of odor of a breeding animal, including. The method of claim 1, wherein the breeding animal is a pig. delete The method of claim 1, wherein the fermentation is performed by mixing pig meal with a microbial medium. delete delete delete