KR101175110B1 - Pharmaceutical composition for the prevention and treatment of viral skin diseases containing extract of Grateloupia filicina and Ceramium kondoi as an active ingredient - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for the prevention and treatment of viral skin diseases, the viral composition comprising a mixed extract of Jinari and silk grass which can improve the overall and effective symptoms of skin diseases accompanying the virus as an active ingredient It relates to a pharmaceutical composition for preventing and treating skin diseases.

Description

Pharmaceutical composition for the prevention and treatment of viral skin diseases containing extract of Grateloupia filicina and Ceramium kondoi as an active ingredient}

The present invention relates to a pharmaceutical composition for the prevention and treatment of viral skin diseases, which contains Jinari and silk grass mixed extract as an active ingredient.

Viruses are the most common cause of disease in humans and are actually widely distributed over millions of years. Skin or mucosal injuries caused by viral infections such as chickenpox, shingles virus, herpes simplex virus, or intestinal virus are frequently found, but no clear cure for these symptoms is established and at present the affected area to prevent secondary infection Antibiotic administration or sterilization with povidone iodine or the like is performed.

There is currently no external application to improve the symptoms of viral infections such as vesicles, wounds or ulcers caused by viral infections such as varicella, shingles virus, herpes simplex virus or enteric virus, while at the same time suppressing pain and itching in these affected areas.

Recently, topical preparations containing antiviral agents or nonsteroidal anti-inflammatory drugs or steroids for symptoms have been developed. However, these viral agents are not satisfactory in effect, and these side effects such as local irritation and redness and side effects such as vesicles, wounds or ulcers It cannot be said that it is sufficient to ameliorate the symptoms of viral infection injury, especially to suppress pain and itching in these affected areas.

In particular, long-term application of steroids has side effects such as hair growth, skin atrophy, skin pigmentation, bacterial infections, acne production, skin thinning, and dermal bleeding. In severe cases, hormonal systemic symptoms may occur, and when the use of the skin disease treatment agent is stopped, symptoms may suddenly worsen and the use of the treatment agent may not be stopped. Therefore, steroid ointment with very low hormone concentration should be used only when it is necessary for a short time and should be used frequently instead of applying large amount at once.

Zinari and silk grass belong to algae, which is a term used to refer to algae that live in the sea, and is classified into green algae, brown algae, and red algae according to the depth and color of the sea. Seaweeds are rich in physiologically active daily acids, fucoidan, laminin, fucosterol, taurine, mannitol, calcium, iodine and vitamins. It is known to have various antibacterial and antiviral effects.

Grateloupia filicina is a seaweed belonging to the genus of the red algae plant in the classification of plants. It is distributed in Korea and all over the world, and its size is 20 ~ 30cm and its width is 2 ~ 3mm. Dark red, very soft, mucus-like fronds, with many side branches on both edges, or deviating. The body shape is severe and the plants belonging to this genus include G. prolongata , G. divaricata , G. okamurai and G. turuturu . (湖 料) and the like.

Silkweed Ceramium kondoi ) is a seahorse belonging to the red silk plants in the class of plants. It is a perennial plant that grows from spring to summer and is used as agar material. Plants are 5 ~ 50cm long, cylindrical, slightly thick, lateral branches, branched one by one, spread out in a fan shape, and small branches alternately in the armpit of upper branches. Branches are composed of a single line of cell populations. The body color is reddish purple, and the darker the darker the color, the older the individual. They live on intertidal rocks in Korea, Japan, Sakhalin, and other algae, and are distributed throughout the coast of Korea, but are commonly found on the southwest coast.

Various health foods and beverages using algae, such as Zinari and silk grass, have been developed in various ways, but it has not been reported that mixed extracts using Genuari and silk grass have an excellent effect on viral skin diseases.

Accordingly, the present inventors have completed the present invention by finding that the composition containing the Jinari and silkweed extract can improve the overall and effective symptoms of skin diseases accompanying the virus than when the extract is used alone. .

An object of the present invention is to provide a pharmaceutical composition for the prevention and treatment of viral skin diseases that can improve the overall and effective symptoms of skin diseases accompanying the virus.

The present invention for achieving the above object, provides a pharmaceutical composition for the prevention and treatment of viral skin diseases, characterized in that the mixed extract of Jinari extract and silk grass extract as an active ingredient.

And the Jinari extract may be used by extracting any one or two or more of the leaves, stems and roots of the Jinuari, the silk grass extract by mixing any one or two or more of the leaves, stems and roots of the silk grass What is extracted can be used.

In the mixed extract, the mixing ratio of Jinari extract and silk grass extract is preferably 9: 1 to 1: 9, preferably 8: 2 to 5: 5, and more preferably 7.5: 2.5 to 6.5: 3.5. .

And the Jinari and silk grass extract is preferably a hot water extract.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for the prevention and treatment of viral skin diseases, which contains the Jinari and silk grass mixed extract as an active ingredient.

In the composition according to the present invention, the mixing ratio of the Jinari extract and the silk grass extract of the mixed extract is preferably 9: 1 to 1: 9. If the mixing ratio is out of the range, there is no significant difference in antiviral response or antiviral inhibitory effect when compared to the case of using the Jinari extract or the silk extract alone. Furthermore, it is more preferable that the said mixing ratio is 8: 2-5: 5, and it is most preferable that it is 7.5: 2.5-6.5: 3.5. In the above composition ratio, the effect of inhibiting viral activity is best.

The Jinari or silkweed extracts according to the present invention may be those extracted or commercially available according to methods well known in the art. In addition, Jinari and silk grass can be used without limitation, such as cultivated or commercially available, and cleanly used. The dried jinari and silk grass are crushed to a suitable size and placed in an extraction container and an appropriate amount of distilled water is added. After extracting it for 1 to 10 hours or more at a suitable temperature, it is possible to obtain a hydrothermal extract according to the present invention by filtering with a filter paper.

Preferably, the hot water extract uses distilled water as an extraction solvent, and more preferably, uses tertiary distilled water. When hot water extraction is preferably heated for 1 to 10 hours at a temperature of 80 ~ 120 ℃.

Since the extract prepared by the above method is a natural ingredient, it is not irritating to the human body and can be effectively used for the prevention and treatment of viral skin diseases.

Therefore, the pharmaceutical composition according to the present invention can be useful for the prevention and treatment of viral skin diseases such as herpes simplex, herpes zoster and psoriasis, contact allergic dermatitis, urticaria.

A pharmaceutical composition of the present invention having a prophylactic and therapeutic effect of viral skin diseases is conventionally prepared by including the above mixture of Jinari and silkweed alone or by additionally including one or more pharmaceutically acceptable carriers, excipients or diluents. It can be formulated in a phosphorous manner.

As used herein, "pharmaceutically acceptable" refers to a composition that is physiologically acceptable and that, when administered to a human, typically does not cause an allergic reaction such as gastrointestinal disorders, dizziness, or the like. In addition, the pharmaceutical composition of the present invention formulated as described above may be administered for the purpose of preventing or treating viral skin diseases through a suitable route of administration. Suitable routes of administration may include several routes including oral, transdermal and the like.

The pharmaceutical composition of the present invention may be formulated as a preparation for oral or parenteral administration according to the selected route of administration, and when formulated, the fillers, weights, binders, wetting agents, disintegrating agents, surfactants, etc. which are commonly used It is prepared using diluent or excipient. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, sucrose, and the like. (Sucrose) or lactose (Lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, and syrups, and may include various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspending solvent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like can be used. Witepsol, macrogol, Tween 61, cacao butter, laurin butter, glycerogelatin, and the like may be used as the base of the suppository.

Jinari and silk grass mixed extract according to the present invention is preferably included in 0.1 to 50% by weight relative to the total weight of the composition. However, the composition as described above is not necessarily limited thereto, and may vary according to the condition of the patient, the type of disease, and the degree of progression.

Preferred dosages of the Jinari and silkweed blend extracts according to the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, it is good to administer at 0.01 mg / kg to 10 g / kg per day. The administration may be carried out once a day or divided into several doses. Thus, the dosage amounts are not intended to limit the scope of the invention in any manner.

The composition according to the present invention can be administered to mammals such as rats, mice, livestock, humans, etc. by various routes. All modes of administration can be expected, for example by oral, rectal, or intravenous, intramuscular, subcutaneous, intrauterine dural or Intracerebroventricular injection.

The composition according to the present invention can be used alone or in combination with methods using surgery, hormonal therapy, drug treatment and biological response modifiers for the prevention and treatment of viral skin diseases.

In another aspect, the present invention provides a cosmetic composition containing the above-mentioned Jinari and silk grass mixed extract as an active ingredient.

Cosmetics prepared from the cosmetic composition for the prevention and treatment of viral skin diseases may be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to the cosmetics containing the mixed extract and silkworm of the present invention, by containing a dermatologically acceptable medium or base may be prepared in the form of adjuvants that can be applied topically or systemically applied commonly used in the field of dermatology. .

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, a cream, a skin, a lotion, a powder, a haze, a spray or a conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the mixture of extracts and silkworms, fatty substances, organic solvents, solubilizers and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And any other ingredients that may be used, such as auxiliaries commonly used in the cosmetic or dermatological arts. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, gel, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, Packs, soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Jinari and silk grass extract according to the present invention is almost non-toxic, exhibits the prevention and treatment of viral skin diseases by antimicrobial and antiviral effects, each of which is mixed with an appropriate ratio of viral skin when applied alone Since there is an excellent effect on the disease, the pharmaceutical composition containing the mixed extract as an active ingredient may be usefully used to prevent and treat viral skin diseases.

1 is a graph showing the cell viability obtained through the cytotoxicity test of the mixture of Jinhua and silk grass according to the present invention,
Figure 2 is a graph obtained by measuring the herpes virus inhibitory effect of the Jinari and silk grass mixture extract of Jinuari and silk grass mixture according to the present invention.

Hereinafter, the present invention will be described in detail through production examples, examples and formulation examples. However, the following Preparation Examples, Examples and Formulation Examples are provided only to specifically describe the present invention, but the scope of the present invention is not limited by these Preparation Examples, Examples and Formulation Examples.

< Manufacturing example  1-6> Zinari  And Silk grass  Mixed Extract Manufacturing

Both hay and silk grass were purchased on the market, and each was heated to 80 ° C. for 24 hours after the addition of tertiary distilled water at a concentration of 3--50 g / 1 L. Thereafter, the mixture was heated to 121 ° C. for another 20 minutes, after sterilization, the solids were filtered and each extract was obtained. In the filtered extract, the ratio of Jinari and Silkweed extract was 9: 1 (Preparation Example 1), 8: 2 (Preparation Example 2), 7: 3 (Preparation Example 3), 5: 5 (Preparation Example 4), 3: 7 (Preparation Example 5) and 2: 8 (Preparation Example 6) were mixed to prepare a mixed extract. Each of the prepared mixtures was used by dissolving the freeze-dried powder in a suitable solvent as needed.

< Comparative Production Example  1>

The mixture was prepared in the same manner as in Preparation Examples 1 to 6, except that the mixing ratio of the Jinhua and the silk grass mixed extract was 10: 0.

< Comparative Production Example  2>

The mixture was prepared in the same manner as in Preparation Examples 1 to 6, except that the mixing ratio of Zinary sap and silk grass mixed extract was 0:10.

The following experiments were conducted to investigate the effect of the mixture of Jinua and silk grass.

< Example  1> Zinari  And Silk grass  Cytotoxicity Test of Mixed Extracts

Dead skin cells  Cytotoxicity Test of Mixed Extracts on Progenitor Cells

HaCaT cells, which are human keratinocytes, were cultured with 10% FBS / DMEM. After seeding with 5 × 10 ³ in a 96 well plate and after 24 hours, 100 μl of the mixed extract of Preparation Examples 1 to 6 was treated for 24 hours. After completion of the mixed extract treatment, the reaction was performed using a CCK-8 kit, and the absorbance was measured at 450 nm of the spectrophotometer to measure the cytotoxicity of the natural mixed extract. As a control group, an untreated group was used, and as a comparative group, extracts of Comparative Preparation Examples 1 and 2 were used.

As shown in Figure 1, when the mixed extract is 10 μg / mL concentration, the cell viability was 93 ~ 98%, respectively, compared to the control group did not show cytotoxicity in the mixed extract of Preparation Examples 1-6. 1 is a control group, b is Comparative Preparation Example 1, c is Preparation Example 1, d is Preparation Example 2, e is Preparation Example 3, f is Preparation Example 4, g is Preparation Example 5, h is Preparation Example 6, i is comparative example 2.

< Example  2> Zinari  And Silk grass  Determination of Herpes Virus Inhibitory Effect of Mixed Extracts

First, uniformly inoculate Vero cells (Verox cells, 3.5X104 cells / ml) in 96-well microplates and stabilize them overnight in a CO ₂ incubator, then remove the culture solution from 96 wells and add 100 μl of HSV with a titer of 100TCID50. Inoculated and adsorbed at 37 ° C. for 1 hour, and then the virus solution was removed. Then, 50 μl of the extract of Preparation Examples 1 to 6 and Comparative Preparation Example 1 and 2 were dispensed into each well. 150 μl was aliquoted, and absorbance (OD) was measured at 540 nm by incubating at 37 ° C. for 3 days. In addition, the control group was used as an untreated group, the extract of Comparative Preparation Examples 1 and 2 was used as a comparative group.

As a result, the virus inhibitory ability of each extract mixture was excellent in herpes virus inhibitory effect, as shown in Figure 2 below, and the inhibitory effect in the case of the mixture of the extract treated with the treatment of Jinari or silk grass extract alone It can be seen that excellent. In particular, it can be seen that in the case of Preparation Example 3 in which the mixing ratio of the mixed extract is 7: 3, an inhibitory effect of about 80% or more is shown. 2 is a control group, b is Comparative Preparation Example 1, c is Preparation Example 1, d is Preparation Example 2, e is Preparation Example 3, f is Preparation Example 4, g is Preparation Example 5, h is Preparation Example 6, i is comparative example 2.

Examples of preparations for the compositions of the present invention are illustrated below.

< Formulation example  1> Sanje  Produce

The powders are prepared by mixing the ingredients shown in Table 1 below and filling them in airtight cloths.

Raw material content( mg )  Jinari and Silkweed Extract 20  Lactose 100  Talc 10

< Formulation example  2> Preparation of tablets

The tablets are prepared by mixing the components shown in Table 2 below and then tableting according to a conventional method for preparing tablets.

Raw material content( mg )  Jinari and Silkweed Extract 10  Corn starch 100  Lactose 100  Magnesium Stearate 2

< Formulation example  3> Preparation of capsules

The ingredients shown in Table 3 below are mixed according to a conventional capsule preparation method and filled into gelatin capsules to prepare capsules.

Raw material content( mg )  Jinari and Silkweed Extract 10  Crystalline cellulose 3  Lactose 14.8  Magnesium stearate 0.2

< Formulation example  4> Preparation of injection

According to the conventional method for preparing an injection, it is prepared in an ingredient amount shown in Table 4 per ampule (2 mL).

Raw material content( mg )  Jinari and Silkweed Extract 10  Mannitol 180  Sterile Distilled Water for Injection 2974  Na2HPO4, 12H2O 26

< Formulation example  5> Manufacture of cosmetics

<5-1> Preparation of flexible lotion

Butylene glycol, glycerin, polyoxyethylene (60) cured castor oil, betaine, citric acid, sodium citrate, and preservatives were added to the purified water, followed by stirring and dissolving. To the mixture was added a mixture of chihuari and silkworm extract according to the present invention and sufficiently stirred, and then aged to prepare a flexible lotion containing the mixed extract according to the present invention. Table 5 shows the content of each component.

Raw material Content (w / w%)  Jinari and Silkweed Extract 25.0  Butylene Glycol 3.5  glycerin 2.5  Polyoxyethylene (60) hardening castor oil 0.1  ethanol 2.5  Betaine 1.0  Citric acid 0.01  Sodium citrate 0.03  antiseptic a very small amount  Spices a very small amount  Purified water Balance

<5-2> Preparation of nutritional lotion

Jinari and silkworm extract, butylene glycol, glycerin, carboxyvinyl polymer, arginine, preservative and purified water according to the present invention were heated with stirring at 70 ~ 75 ℃. Squalane, butylene glycol dicaprylate / dicaprate, sorbitan stearate, polysorbate 60, glyceryl stearate, and stearyl glycerate mixture, which were heated by stirring at 75 to 80 ° C. were added thereto, followed by emulsification. While stirring to add a fragrance while cooling to about 45 ℃ while stirring, and then cooled to 30 ℃ and aged to prepare a nutrient cosmetics containing the mixed extract according to the present invention. Table 6 shows the content of each component.

Raw material Content (w / w%)  Jinari and Silkweed Extract 40.0  Butylene Glycol 8.0  glycerin 5.0  Squalane 10.0  Butylene Glycol Dicaprylate / Dicaprate 5.0  Sorbitan stearate 1.5  Polysorbate 60 1.0  Glyceryl stearate 0.5  Stearyl glycyrrhetinate 0.2  Carboxyvinyl polymer 0.1  Arginine 0.1  antiseptic a very small amount  Spices a very small amount  Purified water Balance

<5-3> Preparation of Essence

Cytocyterol, polyglyceryl-2-oleate, ceramide, ceteareth-4 and cholesterol are stirred and mixed, followed by cytocyterol, polyglyceryl-2-oleate, ceramide, ceteareth-4 and cholesterol. After stirring and mixing, emulsified by adding the mixed solution of the mixture of Noir and silkweed extract, dicetyl phosphate, concentrated glycerin and purified water according to the present invention, and when the mixture is cooled to 45 ℃ while stirring, stirring and adding up to 30 ℃ It was cooled and aged. Carboxyvinyl polymer, xanthan gum and preservatives were added thereto, stabilized, and aged to prepare an essence containing the mixed extract according to the present invention. Table 7 shows the content of each component.

Raw material Content (w / w%)  Jinari and Silkweed Extract 10.0  Cytostolol 1.7  Polyglyceryl-2-oleate 1.5  Ceramide 0.7  Setares-4 1.2  cholesterol 1.5  Dicetyl phosphate 0.4  Concentrated glycerin 5.0  Carboxyvinyl polymer 0.2  Xanthan gum 0.2  antiseptic a very small amount  Spices a very small amount  Purified water Balance

Claims (7)

A pharmaceutical composition for the prevention and treatment of herpes viral skin diseases, characterized in that the extract extract of Jinuari and Silkweed extract consists of an active ingredient.
The method of claim 1,
The Jinari extract is a pharmaceutical composition for the prevention and treatment of herpes viral skin diseases, characterized in that extracted by mixing any one or two or more of the leaves, stems and roots of the Jinuari.
The method of claim 2,
The silk grass extract is a pharmaceutical composition for the prevention and treatment of herpes viral skin diseases, characterized in that extracted by mixing any one or two or more of the leaves, stems and roots of silk grass.
The method of claim 1,
The mixed extract is a pharmaceutical composition for the prevention and treatment of herpes viral skin diseases, characterized in that the mixing ratio of Zinua extract and silk grass extract is 9: 1 to 1: 9.
The method of claim 4, wherein
The mixing ratio of the Jinari extract and silk grass extract of the mixed extract is a pharmaceutical composition for the prevention and treatment of herpes viral skin diseases, characterized in that 8: 2 ~ 5: 5.
The method of claim 4, wherein
The mixing ratio of the Jinari extract and silk grass extract of the mixed extract is 7.5: 2.5 ~ 6.5: 3.5 herpes viral skin disease prevention and treatment, characterized in that the pharmaceutical composition.
The method of claim 1,
The genius and silk grass extract is a pharmaceutical composition for the prevention and treatment of herpes viral skin diseases, characterized in that the hot water extract.

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