KR101136464B1 - Manufacturing method of antibacterial composition comprising herbal extract - Google Patents

Abstract

PURPOSE: A method for manufacturing an antibacterial composition containing oriental medicinal extract is provided to prevent infection by pathogen. CONSTITUTION: A method for manufacturing an antibacterial composition containing oriental medicinal extract comprises: a step of extracting 25-35 wt% of Artewise apiacea Hance, 35-45 wt% of Coptis chinensis Franoh, 10-20 wt% of Clerodendron Cyrtophyllum Turcz, 10-20 wt% of Lonicea japonica Thunb, and 1-10 wt% of Glycyrrhiza uralensis Feish using alkali ion water at 40-85°C for 1-4 hours; a step of adding 0.1-70g of calcium oxide with 100ml of filtered extract; a step of stirring and placing at 4-15°C for 12-24 hours; and a step of filtering supernatant.

Description

Manufacturing Method of Antibacterial Composition Comprising Herbal Extract

The present invention relates to a method for producing an antimicrobial composition containing an herbal extract, and in detail, a method for producing an antimicrobial composition in which the antimicrobial active ingredients of chungho, yellow lotus, large blue leaf, gold silver and licorice are effectively extracted using an aqueous alkali solution. It is about.

Recently, wet tissues and toilet papers having various functions are commercially available, but most of them are treated with various bleaches and chemicals after the crushing work is broken using pulp and recycled paper. If these products are used by children or the elderly with weak skin or poor immunity, they can cause various skin diseases such as eczema, herpes simplex, and viral infections.

In order to solve this problem, Korean Patent Laid-Open Publication No. 10-2002-0003489 proposes a wet tissue for infants and babies that contains a composition prepared by extracting red ginseng, yellowish white, larvae, baekseonpi, alum and distilled under reduced pressure. When the active ingredient is extracted by adding alum, the active ingredients of the Chinese medicine form a precipitate by the strong astringent action of the alum, there is a problem that it is impossible to process the smell and color peculiar to the herbal extract. In addition, Korean Patent Laid-Open Publication No. 10-2004-0091915 proposes a method of preparing herbal tissue additives by boiling herbal medicines at 200 to 250 ° C. for 1 to 2 hours in an airtight furnace, but this is performed in a closed container without adding water. As the heating time 200 ~ 250 ℃ corresponds to dry matter, not extraction, there is a problem that the active ingredients of the herbal medicines can be destroyed by the high temperature dry heating method.

On the other hand, Korean Patent Publication No. 10-2004-0092630 relates to the manufacture of wet tissues containing wood vinegar component, wood vinegar is a component varies depending on the material to be produced, wood vinegar is a carcinogenic substance benzopyrene (Benzopyrene) to remove it There is a need for a step, and there is a problem that the odor and coloring treatment peculiar to wood vinegar is not made. In addition, Patent Publication No. 10-2007-0101660 relates to a wet tissue composition containing a mixed extract of birch sap, rhubarb and baby swimming, and the extraction of the active ingredient using water and ethyl alcohol as an extraction solvent is not sufficient. There is a problem.

In addition, Korean Patent Laid-Open Publication No. 10-2009-0064771 relates to a wet tissue having excellent treatment and prevention effect for skin diseases and a method of manufacturing the same, and the leaves of young leaves, undergrowth branches, plum branches, peach trees, mulberry branches, willow branches, bamboo leaves , Which was extracted by adding 20 times the amount of water, gardenia, and the like, was produced by dipping the filtered composition onto a nonwoven fabric, and this also has a problem in that the active ingredient is not sufficiently extracted using water as an extraction solvent.

Accordingly, the present invention has led to the development of a method for producing an antimicrobial composition in which the antimicrobial active ingredients of blue lake, yellow lotus, large blue leaf, gold silver flower and licorice are effectively extracted using an aqueous alkali solution.

It is an object of the present invention to provide a method for producing an antimicrobial composition which can efficiently extract active ingredients from herbal medicines using alkaline ionized water as an extraction solvent.

In addition, the object of the present invention is to use the supernatant filtered by adding calcium oxide to the extracted herbal medicine extract, which has excellent preservation, no side effects, and contains a large amount of antimicrobial ingredients effective in preventing infection from skin diseases or other pathogenic bacteria. It provides a method for producing an antimicrobial composition.

In order to solve the above problems, the present invention is 25 ~ 35% by weight, 35 ~ 45% by weight of yellow lotus, 10 ~ 20% by weight, 10 ~ 20% by weight of silver and silver, 1 ~ 10% by weight licorice pH 7 ~ 10 to 10 ℃ alkaline ionized water as a solvent for extracting for 1 to 4 hours, and based on 100ml of the filtrate extracted with the alkaline ionized water as a solvent to add 0.1 ~ 70g of calcium oxide and stirred After that, the mixture is left for 4 to 15 hours at 12 to 24 hours, further comprising the step of filtering the supernatant. In addition, the alkaline ionized water used as the extraction solvent is preferably alkaline ionized water containing 1 to 30% by weight of glycerin for more efficient extraction.

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According to the production method of the present invention, it is possible to efficiently extract the antimicrobial component which is excellent in preservation, has no side effects, and is effective in preventing infection from skin diseases or other pathogenic bacteria.
The antimicrobial composition containing the herbal extract of the present invention is applied to various types of daily necessities such as wet wipes, toilet paper, diapers, sanitary napkins, soaps, shampoos, toothpastes, and the like to effectively prevent infection from skin diseases or other pathogenic bacteria in daily life. Can be. In addition, by removing the smell and color peculiar to Chinese medicine, household goods containing an antimicrobial composition can be used comfortably and conveniently.

Figure 1-Photograph before the calcium oxide treatment of the herbal extract of the present invention
Figure 2-Photograph after treatment with calcium oxide herbal extract of the present invention
Figure 3-Photo showing antimicrobial powder prepared according to the present invention
Figure 4-Photo showing wet wipes made in accordance with the present invention
Figure 5,6-Photo showing toilet paper made according to the invention

Hereinafter, preferred embodiments of the present invention will be described in detail. Prior to this, terms or words used in the present specification and claims should not be construed as being limited to ordinary or dictionary meanings, but should be construed as meanings and concepts consistent with the technical spirit of the present invention.

First, the antimicrobial composition of the present invention is 25 ~ 35% by weight, 35 ~ 45% by weight of yellow lotus, 10 ~ 20% by weight, 10 ~ 20% by weight of silver and silver, 1 ~ 10% by weight licorice alkali of pH 7-10 It is characterized in that the ionized water as a solvent for extraction for 1 to 4 hours at 40 ~ 85 ℃.

Artewise apiacea Hance is a herbaceous annual herbaceous plant, and is said to be a scent. Abrotanine, Vitamin-A, β-bourbonne, farnesul, acetate, caryophyllene, ß-humulene, ε-cadinene, δ-cadinene, α-pinene, β-pinene, camphene, lymonene, ε-cadinene, α-pinene 1.8-cineole It contains active ingredients such as α-thujone, copaene, γ-cadinene, and is generally effective for malignant boils, pruritus, dysentery, promotion of granulation, non-bleeding, bleeding, toothaches, and insect bites.

In addition, the Coconut chinensis Franoh (Coptis chinensis Franoh) is a perennial herbaceous plant Ranunculus (Ranun culaceae) is a rhizome removed from the roots of the nasturtium. It contains active ingredients such as Berberine, Coptisine, Worenine, Palmatine, Columbamine, Obakunune, Obakulactone, and is commonly used for meningitis, pneumococci, anthrax, cholera, staphylococci, erythrocytes, jyptheria, Bacillus subtilis, pertussis, pests, It is effective for tuberculosis bacteria and tetanus bacteria.

In addition, the larva (Lonicea japonica Thunb) is a perennial vine shrub as a plant flower (Lonicea), both leaves and stem flowers are used for medicinal. It contains active ingredients such as luteolin, imositol, saponin, tanmin, etc., and is generally effective for Escherichia coli, green leaf bacillus, diffus bacillus, paradiff bacillus, pertussis bacillus, staphylococcus bacillus, pneumococcus, Salmorera bacilli, erythritis bacillus, and meningitis.

In addition, the chlorophyll (Clerodendron Cyrtophyllum Turcz) is a deciduous shrub with Verbena and plants, containing active ingredients such as Flavonoid, indican, tannin, yellow pigment, tryptophan, isatan-B, glucobrassicin, neoglucobarassicin, glucobrassicin-l-sulfonate It is effective against bacteria resistant to streptomycin, nitrofuurazone, sulfathiazole, berberine, meningitis and dysentery.

On the other hand, the licorice (Glycyrrhiza uralensis Feish) is a perennial herbaceous root of the legume (Leguminaceae) plants, triterpenoids, saponins glycyrrhizic acid, glycyrrhizen, 18-α-glycyrhetinic acid, flavone, liavone, liquirrtgenin (4'- 7-dihydroxyflavone), isohiquirrtigenin, liquiritin (liquirit-gennin-4'-β-glucoside), neohiquiritin (isohiquiritgenin-7-β-glucoside), neoisoliquiritin (isohquiritigenin-4-β-glucoside), isoliquiri tigenin-4-β- It contains active ingredients such as glucosid-β-apioside and is effective in staphylococcus, dysentery, tuberculosis, detoxification, neurostabilization, gastritis, asthma, skin eczema, anti-inflammatory and anti-ulcer.

In particular, the triterphenoide, Glycyrrihizic acid component of licorice increases the antibacterial activity by the cooperative action of Berberine and tannin and Luteolin component in nasturtium, licorice component is similar to the steroid.

The present invention uses alkaline ionized water as a solvent, unlike the prior art, in order to efficiently extract the herbal medicines having the above combination. Alkaline ionized water has a smaller molecular cluster (cluster) than water, and thus has good permeability, high thermal conductivity, and high solubility, so that the component is not only efficiently dissolved at a relatively low temperature but also has excellent absorption.

Generally, when extracting herbal medicines, it is common to extract purified water or purified water and alcohol in a predetermined amount for 4 to 5 hours at a temperature of 90 to 100 ° C. or more. However, the weak components of heat were often decomposed. .

However, in the present invention, by using the alkaline ionized water as a solvent, the herbal ingredient can be safely extracted at a relatively low temperature to sufficiently exhibit the active function of the extract, and the clusters form hydrogen bonds in 13 to 15 units. Unlike existing water, alkaline ionized water is composed of 6 clusters, which has excellent absorption and melting power.

Furthermore, the present invention by adding 1 to 30% by weight of glycerin to the alkaline ionized water, by applying the osmotic pressure between the solute and the solvent to ensure that the active ingredient dissolution is good and safe, and when applied to the skin moisturizing The effect was obtained.

The elution amount of the herbal medicine extracted with distilled water and alkaline ionized water (pH 7.5) containing 3% glycerin was compared as follows.

[ Example  1] Comparative experiment of elution amount using distilled water and glycerin alkaline ionized water

In the embodiment of the present invention, 15 g of Cheongho, 20 g of yellow lotus, 14 g of large blue leaves, 14 g of gold and silver liquor, and 5 g of licorice are taken as fine powder, and 30 ml of 3% glycerin alkaline ionized water (PH.7.5) is added thereto at 75 ° C. After heating for 3 hours, the mixture was filtered and left for 2 hours in a constant temperature room adjusted to 25 ° C., cooled, and then precisely taken with 10 ml of micro-pipette and cooled to prepare an experimental example.

In Comparative Example, 30 g of the same medicinal herb was precisely taken, 50 ml of distilled water was added thereto, heated at 75 ° C. for 3 hours, filtered, and left for 2 hours in a constant temperature room adjusted to 25 ° C., followed by refreshing 10 ml accurately with a micropipette. The same experiment was repeated five times. The dissolution test results of Example 1 and Comparative Example are shown in Table 1 below.

Recall / Condition Example Comparative Example 1 One 10.1737 10.1703 2 10.1825 10.1695 3 10.1770 10.1713 4 10.1643 10.1583 5 10.1701 10.1640 Average 10.1723 10.1679 Remarks
3% glycerine specific gravity 0.0014 and 0.00014 total mineral content in alkaline ionized water (PH7.8) were not added.

As can be seen from the above results, it can be seen that the extraction amount is increased when distilled water is used when extracted with glycerin alkaline ionized water.

On the other hand, in order to improve the antimicrobial activity of the extract medicinal herb extract and to solve the odor and coloring problems, 0.1 ~ 70g of calcium oxide based on 100ml of the filtrate extracted with the alkaline ionized water as a solvent and stirred, It may further comprise the step of filtering the supernatant by standing for 12 to 24 hours at 4 ~ 15 ℃.

The calcium oxide is a dense lump-like mineral mainly made of calcite, mostly white or off-white. The calcium oxide becomes hydrated lime (Ca (OH) ₂ ) upon absorption of water, and is generally effective for insecticides, hemostasis, eczema, skin pruritus, burn healing, wound bleeding, hemorrhoids, and antidetermination.

The composition of the herbal extract before and after the treatment with calcium oxide has a marked difference in color and odor as shown in FIGS. 1 and 2. In other words, the color becomes colorless and the smell of herbal medicine disappears. In addition, the antimicrobial activity before and after the treatment with calcium oxide is also different as can be seen in Example 2.

[ Example  2] Comparison of antimicrobial activity before and after calcium oxide treatment

The strain used for the experiment was inoculated in Luria-Bertain liquid medium, and 12 hours later, a sample of late logarithm was prepared and used. After spreading the strain evenly in Luria-Bertani medium, put the sterilized disk and dispense 1μm strain in the liquid medium and incubate the experimental medium for one day at 37 ℃ incubator week, and then determine the bacterial growth inhibitory ability around the disk 2 is shown. (1-A: before Chinese medicine extract Cao treatment, 1-B: after Chinese medicine extract Cao treatment, C-1: calcium oxide water)

Strain / Sample 1-A 1-B 1-C Bucillus amyloiquefaciens FZB42 - - - Bacillus cereus - + - Bacillu korlensis - ++ - Bacillus stratosphericus + + - Bordetella trematum - - - Enteroccous avium + ++ - Enterococcus faecalis - - - Enterococcus pellens - + - Providencia rettgeri - - - Klebiella pneumoniae Subsp. ozaenae - + - Citrobacter farmei - - - Micrococcus Yunnanensis - - - Proteus mirabilis - - - Bacillus safensis + - - Staphylococcus aureas + ++ - Bucillus Stratosphericus + ++ - Paebibacillus sp. ++ - - Shigella boydii - ++ - Streptococcus mutans + - -

As can be seen from the results, it can be seen that the antimicrobial activity of the composition increases after calcium oxide treatment.

On the other hand, the antimicrobial composition described above may be utilized by adding to various household goods such as diapers, sanitary napkins, soaps, toothpastes, shampoos, wet tissues, toilet paper, and the like, and are prepared in powder form as shown in FIG. Can be used for In this case, the antimicrobial powder may be easily prepared by spray drying or drying at a low pressure under reduced pressure of the antimicrobial composition.

The antimicrobial wipes using the antimicrobial composition of the present invention is diluted after the antimicrobial composition is diluted to 3 to 5 vol% aqueous solution, and then sprayed or adsorbed onto the nonwoven fabric (15x20cm, 1 sheet / 10 to 15 ml), and then sealed after UV sterilization. It can be manufactured by packaging. At this time, a trace amount and a moisturizing agent may be added.

In addition, the tissue using the antimicrobial composition of the present invention may be prepared by diluting the antimicrobial composition with an aqueous solution of 3 to 5 vol%, and then spraying the prepared aqueous solution with 15-20 ml of a piece of paper fabric (60x60 cm) after drying. . At this time, the paper fabric is first crushed 50g paper, 700 ~ 800ml of purified water with water in the crusher and then filtered with 100 ~ 150mesh wire mesh, 500ml of sterile purified water is added to the precipitate and stirred for 10 to 20 minutes and 100 ~ 150mesh wire mesh When the filtrate is filtered again, the chlorine ions are not detected, the precipitate is taken, and 500 ml of sterile distilled water is added to make a suspension solution, which can be prepared by uniformly spreading on a 100 ~ 150 mesh wire mesh (60x60cm) cloth and drying.

The wet tissues and toilet papers thus prepared are effective for preventing hemorrhoids, prolapse, eczema, and trichomonas infection by cleaning the perianal area and preventing infection. Hereinafter, a process of manufacturing a wet tissue and toilet paper according to an embodiment of the present invention will be described.

[ Example  3] Preparation of wet tissue containing antimicrobial composition

Cheongho 30g, rhubarb 40g, 14g gold and silver ginseng 14g, licorice 5g is selected and seasoned, and 700ml of 3% glycerin alkaline ionized water (manufacturer Human Water, Hu-50, pH 7.5 ~ 8.0) is added at 75 ~ 85 ℃. After extracting for 3-4 hours, 200 ml of the filtrate was filtered, 40 g of calcium oxide (Lime Stone, CaO) was added thereto, stirred at room temperature for 10 minutes, left at 4-5 ° C. for 12 to 24 hours, the supernatant was taken out and filtered again. Chinese medicine extract composition was prepared by adding 3-5 ml of herbal extract to 100 ml. 10-15 ml of the 15X20cm buried nonwoven fabric, which was sterilized by the Chinese herbal extract composition, was spray-absorbed or immersed in an airtight package to prepare a wet tissue as shown in FIG. 4.

[ Example  4] Preparation of toilet paper containing antimicrobial composition

50 g of decolorized recycled paper was added to 600 ml of purified water, and then pulverized with water in a grinder and filtered through a 150 to 200 mesh wire mesh. 500 ml of sterile purified water was added to the precipitate, followed by stirring for 10 to 20 minutes (rpm 80 to 100). If the filtrate is neutral and no chlorine ion is detected by filtration through ~ 200mesh wire mesh, stop the washing process, take the washed wet paper material into a separate container, add 500ml of sterile purified water, and stir to give a solution of suspension solution (60X60cm ) Spread uniformly on cloth and dry it to prepare paper fabric, and add 5 ~ 10ml of the composition of Example 3 to 100ml of distilled water (5 ~ 10%) (60x60cm) buried / spray 15 ~ 20ml, deposit and dry at low temperature. 5 to 6 to prepare a toilet paper.

The present invention is not limited to the above-described specific embodiments and descriptions, and various modifications can be made to those skilled in the art without departing from the gist of the present invention claimed in the claims. And such modifications are within the scope of protection of the present invention.

Claims (8)

Cheongho 25-35% by weight, rhubarb 35-45% by weight, large blue leaf 10-20% by weight, gold silver coin 10-20% by weight, licorice 1-10% by weight 40-85 using alkaline ionized water of pH 7-10 as a solvent Extracting at 1 ° C. for 4 hours;
Chinese medicine further comprising the step of filtering the supernatant by adding 0.1 to 70 g of calcium oxide on the basis of 100 ml of the filtrate extracted with the alkaline ionized water as a solvent, and stirring the mixture for 10 to 24 hours. Method for producing an antimicrobial composition containing an extract. delete The method of claim 1,
Said alkaline ionized water is alkaline ionized water containing 1-30 weight% glycerin, The manufacturing method of the antimicrobial composition containing the herbal extract. delete delete delete delete delete

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