KR101126385B1 - Method of making composite for skin immunity improvement - Google Patents

Abstract

The present invention relates to a method for preparing the skin immunoenhancing composition. More specifically, mistletoe is distilled, filtered through a membrane filter, vacuum compressed, and sterilized to prepare a skin immune enhancing composition to supply moisture to infants' and children's skin to prevent and treat dry skin and increase skin immunity. It can be used to improve the skin immunity of the mistletoe by preparing the skin immune-enhancing composition by mixing the mistletoe with yellow lead, golden, yellow and white gardenia, and then distilling or mixing the mistletoe with dandelion, dandelion and broiler. Not only does not burden the organs, but also prevents vomiting, and prepares a reverse phase extraction tea and heats it in a separate extraction tank and heats it. It relates to a manufacturing method.
To this end, the present invention comprises the steps of (a) harvesting the mistletoe containing Lectin, Viscotoxin, Polysaccharide, Alkaioid, Oleanolic acid components in October-February; (b) washing the mistletoe with running water to remove moisture; (c) cutting off the mistletoe from which moisture has been removed; (d) mixing the chopped mistletoe with distilled water; (e) distilling the mixture into a container by heating; (f) filtering the distilled solution with a membrane filter; (g) placing the filtered solution in a Vial container and sealing it; And (h) provides a method for producing a skin immuno-enhancing composition comprising the step of sterile sterilization after vacuum compression of the sealed vial container.

Description

Method of making skin immune enhancing composition {Method of making composite for skin immunity improvement}

The present invention relates to a method for preparing the skin immunoenhancing composition. More specifically, mistletoe is distilled, filtered through a membrane filter, vacuum compressed, and sterilized to prepare a skin immune enhancing composition to supply moisture to infants' and children's skin to prevent and treat dry skin and increase skin immunity. It can be used to improve the skin immunity of the mistletoe by preparing the skin immune-enhancing composition by mixing the mistletoe with yellow lead, golden, yellow and white gardenia, and then distilling or mixing the mistletoe with dandelion, dandelion and broiler. Not only does not burden the organs, but also prevents vomiting, and prepares a reverse phase extraction tea and heats it in a separate extraction tank and heats it. It relates to a manufacturing method.

Immunity refers to a phenomenon in which the internal environment of a living body defends against an antigen, which is an external factor, and is classified into innate immunity and acquired immunity acquired after birth.

Innate immunity, also known as 'natural immunity', is a nonspecific reaction to antigens and does not have a special memory function. Innate immune systems include skin, mucous tissue, acidic acid, and complement that are present in the blood to block antigen invasion. Cells include macrophages responsible for phagocytosis, polymorpho nuclear leukocytes, and K cells that can kill infected cells. In fact, most infections are protected by this innate immunity.

On the other hand, acquired immunity is also referred to as 'acquired immunity', because it can remember the first invading antigen and has a characteristic that can effectively remove the antigen by reacting specifically when reinvading, it serves to reinforce innate immunity.

Acquired immunity is divided into humoral immunity and cell-mediated immunity for convenience.

Humoral immunity is differentiated after B lymphocytes recognize antigens to secrete antibodies, which mainly function to remove infected bacteria. Antibodies exist in body fluids and consist of a glycoprotein called immunoglobulin (Ig).

Cellular immunity plays a role in the thymus-derived T lymphocytes recognizing antigens to secrete lymphokine or to directly kill infected cells. Secreted lymphokines may also activate phagocytes to aid phagocytosis. Such cellular immunity primarily functions to remove cells infected with viruses or bacteria that can grow within cells.

When immune function is reduced, allergy and hypersensitivity may be caused. Allergic reactions such as allergy can be divided into four types based on expression time and response type.

Type I (immediate) is typical of most allergic reactions, including symptoms of asthma, seasonal or perennial rhinitis, allergic sinusitis, conjunctivitis, food and drug allergies, atopic dermatitis, hives and bee stings Reaction and the like. Type II (antibody involvement) causes cytolytic or inflammatory reactions of humoral immunity. Symptoms include hemolysis of red blood cells due to inadequate transfusion, and neonatal hemolytic disease. Type III is caused by reactions by antigen-antibody binding or by antigens of small molecular weight. Causes acute glomerulonephritis, irritable pneumonia. Finally, type IV (delayed) is a type of cellular immune response induced by T lymphocytes and macrophages that causes important reactions such as tuberculin reactions against M. tuberculosis, transplant rejection, and invasion by microorganisms such as viruses. .

In particular, allergies, sensitive skin, atopic dermatitis and skin diseases, including various eczema has become a big problem is increasing interest in skin immunity. Infants and children, in particular, have a tender, soft skin that is very sensitive to external conditions and irritation. This is due to the widespread use of artificial pigments and chemical seasonings in the diet, frequent consumption of oily fast foods, and exposure to various pollutions, including fine dust, which constantly irritate fragile skin. Rural space in the city is gradually decreasing, and the central heating concrete apartment environment is too dry to dehydrate the weak child's skin. In addition, as I spend a lot of time at home, the skin immune system is weakened.

Therefore, in particular, it is urgent to develop a substance that can supply skin to infants and children to prevent and treat dry skin and increase skin immunity.

The present invention has been made to solve the above problems, by supplying moisture to the skin of infants and children to prevent and treat dry skin and increase the skin immunity, while doubling the skin immune effect of mistletoe It is an object of the present invention to provide a method for preparing a skin-immune-enhancing composition which can not only induce vomiting due to a burden on an organ, but also obtain a gel-like high molecular substance as an active ingredient crystal.

Method for producing a skin immune enhancing composition according to the present invention devised to achieve the above object is (a) harvesting a mistletoe containing Lectin, Viscotoxin, Polysaccharide, Alkaioid, Oleanolic acid components in October-February; (b) washing the mistletoe with running water to remove moisture; (c) cutting off the mistletoe from which moisture has been removed; (d) mixing the chopped mistletoe with distilled water; (e) distilling the mixture into a container by heating; (f) filtering the distilled solution with a membrane filter; (g) placing the filtered solution in a Vial container and sealing it; And (h) sterilizing and disinfecting the sealed vial container under vacuum.

In addition, the manufacturing method may be to mix the mistletoe 100g and distilled water 400mL in step (d).

In addition, the manufacturing method is preferably heated to 80 ~ 120 minutes at 90 ~ 110 ° C in step (e).

In addition, the filtering scale of the membrane filter in the step (f) is preferably 0.1 ~ 0.3㎛.

In addition, the manufacturing method may further comprise the step of sterilizing sterilization of the Vial bottle for 10 minutes at 120 ~ 130 degrees between step (e) and (f).

In addition, the manufacturing method may further add sulfur lead, gold, yellow white, gardenia to the mistletoe and distilled water in step (d).

In addition, the step (d) may be mixing the distilled water 500mL in each of the mistletoe and sulfur lead, gold, yellow white, gardenia each 50g.

In addition, the manufacturing method may be to add the yellow, dandelion, broiler to the mistletoe and distilled water in step (d).

In addition, the step (d) may be to mix 100ml of the distilled water in the mistletoe 5g, yellow gold 5g, dandelion 5g, broiler 3g.

According to the present invention, the mistletoe is distilled, filtered through a membrane filter, vacuum compressed and sterilized to prepare a skin immune enhancing composition, thereby supplying moisture to the skin of infants and children to prevent and treat dry skin and increase skin immunity. It can be effected.

In addition, according to the present invention by distillation after mixing hwangyeon, golden, yellow and white, gardenia in the mistletoe, or by distillation after mixing hwangjeong, dandelion, broiler on the mistletoe, by distillation to produce a skin immune enhancing composition, the skin immune effect of mistletoe While doubling the burden does not burden the user's organs are effective in preventing vomiting.

In addition, according to the present invention, by preparing a reverse phase extraction difference and then heating it in a separate extraction tank, it is possible to obtain a gel polymer material which is a crystal of the active ingredient.

1 is a flow chart of a method for producing a skin immune enhancing composition according to a first embodiment of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. First, in adding reference numerals to the components of each drawing, it should be noted that the same reference numerals are assigned to the same components as much as possible, even if shown on different drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. In addition, the following will describe a preferred embodiment of the present invention, but the technical idea of the present invention is not limited thereto and may be variously modified and modified by those skilled in the art.

Mistletoe contains more than 1,700 kinds of ingredients such as polysaccharides, alkaioids, and oleanolic acid, as well as anti-active ingredients such as lectin (molecular weight 60,000 daltons) and biscotoxin (5,000 daltons molecular weight). These components are known to work in combination in the body. The most important component of mistletoe is lectin, which kills harmful cells and forms beta endorphins. Biscotoxin necroses harmful cells at high doses and enhances immunity at low doses. In addition, peptides have an antitumor effect, polysaccharides increase the activity of immune cells, NK cells and LAK cells, and sopoids have a potent effect on the growth of helper T cells.

1 is a flow chart of a method for producing a skin immune enhancing composition according to a first embodiment of the present invention.

Mistletoe (Science name: VISCUM ALBUM, Ingredient: Lectin, Viscotoxin, Polysaccharide, Alkaioid, Oleanolic acid, and other 1700 species) are collected between October and February (S10), washed with running water, and water is removed (S20). do.

Finely cut the mistletoe removed moisture (S30).

The cut mistletoe and distilled water are mixed (S40), but the mixing ratio is preferably a ratio of 300 to 500 mL of distilled water to 100 g of mistletoe. If the mixture of distilled water in less than 300mL of mistletoe is too low concentration of mistletoe to reduce the skin immunity and skin moisturizing effect, if more than 500mL there is a problem that may cause side effects such as vomiting due to excessive concentration of mistletoe.

Put the mixture of mistletoe and distilled water in a container and heat it for 80 to 120 minutes at 90 ~ 110 ° C to perform distillation extraction (S50). Distillation refers to the process of heating a solution in which a solute is dissolved to reach a boiling point, producing a gaseous substance, and cooling it again to obtain a pure liquid. In azeotropic mixtures or mixtures with near boiling points, a solvent having a high affinity component is added to only one component to lower the vapor partial pressure of the component, thereby making the difference between the vapor composition and the liquid composition large. At this time, a method of adding a relatively low volatility solvent is distillation, a method of adding a relatively high volatility solvent is called azeotropic distillation.

At this time, when the heating temperature is less than 90 degrees Celsius, the distillation process is difficult to proceed because the distilled water is not evaporated smoothly. If the heating temperature is higher than 110 degrees Celsius, the distilled water vaporization in the mixture is excessively activated, and the non-mistola component is evaporated without sufficient concern. There is a problem. In addition, if the heating time is less than 80 minutes, there is a limit to the extraction of various beneficial components of the bistolto is not enough extraction is made, the quality of the extract is lowered, if it exceeds 120 minutes, the extract is too concentrated, the amount of the extract is less There is an aspect of wasting energy.

Sterilize (S60) the Vial bottle at 120-130 degrees for approximately 10 minutes in advance. If the heating temperature is less than 120 degrees Celsius, it is difficult to achieve sufficient sterilization, and if it exceeds 130 degrees Celsius, there is a problem that the efficiency of heating is lowered compared to the fuel consumption.

Distilled solution is filtered through a membrane filter (membrane filter) and then put in a sterilized sterilized Vial container and sealed by vacuum compression (S80) and used after sterilization. At this time, it is preferable that it is 0.1-0.3 micrometer, and, as for a membrane filter, it is more preferable that it is 0.2 micrometer. If the filtering scale of the filter is less than 0.1㎛ it is difficult to achieve the object of the present invention by increasing the components that do not pass through the membrane filter of the active ingredients distilled, it is difficult to achieve a finer nanoscale particle state than 0.3㎛ There is a problem.

As a result of the clinical trial, the first example showed excellent efficacy in skin immunity enhancement, in particular in skin moisturizing.

Collect mistletoe between October and February, wash it under running water, remove moisture, and cut finely. Along with this, yellow lead, golden, yellow white, and gardenia are washed in running water, water is removed, and finely cut.

Hwang Yeon (Coptis chinensis C. Y. cheng, Coptis chinensis Franch, Coptis Japonica Makino) contains Berberine, Worenine and other Polnitine Co-ptisine.

Gold (Scutellaria baicalensis Georgi) contains Wogonin baicalin. Golden is a perennial herb of dicotyledon and moss plant, grows in grass of mountain area. The leaves are opposite each other, lanceolate, with flat edges. Corollas curled at the bottom, stand straight, tubular, 1.5-2.5cm long, and have a lip shape. The fruit is round and contained in the calyx. The cone is conical and the flesh is yellow.

Hwangbaek (scientific name: Phellodendron amurense Ruprecht) is a dried bark of the yellow bark belonging to the rutaceae, also known as the hwangpyeongpi. It is a deciduous broad-leaved arboreous tree, growing up to 10m in height. Cork is developed in the bark, and the cuticle is yellow. The leaf is a pinnate leaf, and the small leaf is 5 ~ 13 sheets, and it is an egg-shaped lanceolate or a long circle. The leaf edge has small jagged and soft hairs, and the base of the middle vein has some long hairs. The fruit has a lot of berry and has a hard core in the center, and when rounded, it is purple-black. In Korea, it is distributed in all regions except Jeju, Jeonnam, and Japan, Manchuria, China, Amur, and Usuri. It grows in scrub forests or valleys in the mountains. Peel the bark from the trunk before or after the summer to remove the rough skin or cut it and dry it in the sun. When taken, palpation of gastric juice is promoted by palpation of taste reflexes, which also leads to an increase in appetite.

Gardenia is the evergreen shrub of the dicotyledonous plywood grouped in the locusts, and contains Gardenin-crocetin and Essential.

50 g of each of mistletoe, sulfur lead, gold, yellow white and gardenia and 500 mL of distilled water are mixed and distilled extraction is performed. In the distillation extraction process, the mixture is placed in a container and heated to 90 to 120 degrees for 80 to 120 minutes. Next, the vial bottle is heated at 120 to 130 ° C. for 10 minutes to perform sterile disinfection. The distilled solution is filtered with a membrane filter (0.2㎛), and then placed in a vial container, sealed, vacuum compressed, and sterilized and used.

As a result of the clinical trial, the second example exhibited excellent efficacy on skin immunity, in particular on skin allergy.

Prepare mistletoes, yellow flowers, dandelions and broilers.

Hwang Jung (Plango Polygonnatum falcatum A. Gray, Falcatum A. Gray) contains sugar, ash and large amount of starch. Hwangjeong is a perennial herb of the monocotyledonous genus Liliumaceae. The thick fleshy rhizome extends to the side, and the stem has six ridges, and the tip is obliquely slanted. Fruits are round with berry and ripen black from September to October.

Dandelion (Taraxacum platycarpum Dahlsted) is Inulin, Laevulose, Cluytianol, Taraxcin, Taraxacerin, Inosite, Resin, Aspargin, P-Hydroxyphenyl acetic acid, 3,4-Dioxy cinnamic acid, Homotaraxasterol, Taraxasterol, XanthophyllAB, Cholin & vitamine Contains ingredients. Dandelion is a perennial plant of the dicotyledon plant Campanula, which grows in a sunny place in the field. Stems are absent, leaves aggregate from roots and spread laterally. Flowers bloom in April-May, yellow, with one head-shaped flower at the end of the stalk similar in length to the leaf. Fruits are aquatic, long oval, and have thorn-like protrusions on the upper part.

Broiler (Cinnamomum cassia Blume) is essential oil, Cinnamic aldehyde, Camphene, Cineol, Linalool, Eugenol Contains ingredients. Broilers are bark of the Cinnamomum cassia Blume of the camphor tree in Korea and China. The broiler tree is an evergreen and tall tree that grows in southern China. It is collected from summer to autumn and dried in the sun. This drug has an unusual aroma and tastes hot and sweet. Appearance is plate-like or plate-shaped rolled inward. The outer surface is grayish brown, and the fine lines and side pores protrude.

Wash the mistletoe, citrine, dandelion and broiler under running water, remove moisture, and then cut finely. Mistletoe, citrine, dandelion, broiler is mixed with distilled water, mistletoe 5g, 5g yellow, dandelion 5g, broiler 3g, distilled water in a ratio of 100mL. The mixture is placed in a container and heated to 90-100 ° C. for 80-100 minutes to carry out distillation. Sterilize the vial bottle at 120-130 ° C for 10 minutes. The distilled solution was filtered through a membrane filter (0.2㎛), put in a vial container, sealed and vacuum-compressed. Finally, it is sterilized and used.

As a result of the clinical trial, Example 3 showed an excellent effect on skin immunity enhancement.

On the other hand, in the extraction step (S50) it is also possible to manufacture a reverse phase extraction difference by heating to a temperature below the boiling point using an extractor equipped with a pressurized tank. In the above Examples 1 to 3, when the reverse phase extraction difference is manufactured by using the pressurized tank extractor in the extraction step, the concentrated substance in a gel (GEL) state may be prepared by putting the reverse phase extraction difference into a separation extraction tank and heating it. In this way, the GEL concentration step not only completely removes the toxicity but also obtains a thick gel concentrate. Through this process, the highly concentrated gel-like material is removed from the low molecular weight material without the active ingredient, leaving only the polymer material, which is a crystal of the active ingredient.

In addition, in Examples 1 to 3, it can be directly applied to the skin or taken for internal use. Since the filter scale of the membrane filter is a nanoscale of 0.1 ~ 0.3 ㎛ level, it can be easily and quickly absorbed into the body when taken.

The above description is merely illustrative of the technical idea of the present invention, and various modifications, changes, and substitutions may be made by those skilled in the art without departing from the essential characteristics of the present invention. will be. Accordingly, the embodiments disclosed in the present invention and the accompanying drawings are not intended to limit the technical spirit of the present invention but to describe the present invention, and the scope of the technical idea of the present invention is not limited by the embodiments and the accompanying drawings. . The protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent scope should be interpreted as being included in the scope of the present invention.

The present invention can be widely applied to the field of skin hydration and skin immunoenhancing composition.

S10-Harvesting Step S20-Washing / Moisture Removal Step
S30-Cutting step S40-Mixing step
S50-Distillation extraction step S60-Sterilization disinfection step
S70-Filtration stage S80-Sealing / vacuum compression stage

Claims (9)

(a) collecting the mistletoe containing Lectin, Viscotoxin, Polysaccharide, Alkaioid, Oleanolic acid components in October-February;
(b) washing the mistletoe with running water to remove moisture;
(c) cutting off the mistletoe from which moisture has been removed;
(d) mixing the chopped mistletoe with distilled water;
(e) distilling the mixture into a container by heating;
(f) filtering the distilled solution with a membrane filter;
(g) placing the filtered solution in a Vial container and sealing it; And
(h) vacuum sterilizing and sealing the sealed vial container
Method for producing a skin immune enhancing composition comprising a. The method of claim 1,
Method (d) of the skin immunity enhancing composition characterized in that the mixture of 100 g of mistletoe and 400 mL of distilled water. The method of claim 1,
Method for producing a skin immune enhancing composition, characterized in that for heating 80 to 120 minutes to 90 ~ 110 degrees in the step (e). The method of claim 3,
The filtering scale of the membrane filter in the step (f) is 0.1 ~ 0.3 ㎛ manufacturing method of the skin immune enhancing composition, characterized in that. The method of claim 1,
Method of producing a skin immune enhancing composition, characterized in that further comprising the step of sterilizing and disinfecting the vial container for 10 minutes at 120 ~ 130 ° C between step (e) and (f). The method of claim 1,
In the step (d) of the mistletoe and distilled water, sulfuric acid, gold, yellowish white, gardenia method of producing an immune enhancing composition, characterized in that further adding. The method of claim 6,
The step (d) is a method of producing a skin immune enhancing composition, characterized in that the distilled water 500mL mixed with each of the mistletoe and sulfur, golden, yellow and white, gardenia. The method of claim 1,
In the step (d) of the mistletoe and distilled water, yellow cinnamon, dandelion, broiler, characterized in that the method for producing a skin immune enhancing composition further. The method of claim 8,
The step (d) is a method of producing a skin immune enhancing composition, characterized in that the mixture of distilled water 100g to 5g, 5g yellow, 5g dandelion, 3g broiler.

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