KR101091166B1 - Anti-microbial composition comprising methyl-isogugenol or cyclohexanone - Google Patents

Abstract

The present invention relates to an antimicrobial composition comprising methyl-isoeugenol or cyclohexanone.

Description

Antimicrobial composition comprising methyl-isogugenol or cyclohexanone

The present invention relates to an antimicrobial composition comprising methyl-isoeugenol or cyclohexanone.

The antimicrobial agent prevents the production of microorganisms or suppresses the reproduction of industrial antimicrobial agents currently developed about 130 species, in consideration of human safety aspects, it is preferable to use a natural antimicrobial agent extracted from natural organisms.

Natural antimicrobial is a substance that suppresses microbial contamination and growth, and is widely used as food additives, pharmaceuticals, and industrial substances. Currently, cypress extract hinokitiol, magnolia extract megnonol, and grapefruit seed extract DF -100 and the like have been developed, but the scale is only 17% of the synthetic preservatives.

Herbs are edible, medicinal, and fragrances. These are the generic term for fragrant plants that grow on the Mediterranean coast and Southwest Asia, and recently include the leaves, flowers, and fruits of trees.

Essential oils extracted from these herbs are volatile oils with unique fragrances, also called fragrance oils. In particular, there are more than 500 varieties of herb extracts, anise oil, citronella oil, terepin oil, peppermint oil, rosemary oil, etc., and about 100 of them are currently used as raw materials for edible, medicinal and natural flavors.

Essential oils each have a unique scent and have strong antiseptic and antiseptic properties. Essential oils are chemically divided into oxygen-containing compounds such as alcohol aldehydes, ketones, organic acids, esters, and mono and sesquiterpene hydrocarbons. The former is involved in the aroma and taste of essential oils. It is difficult to dissolve in water and is rather an obstacle to aroma or taste.

Essential oils, herbal extracts, have the unique properties and ingredients of natural products, and have been used in various ways until now. In other words, it is used as a therapeutic tool, a spice, a digestive agent, a neuro stabilizer, and an odor inhibitor, as well as a beauty healing tool to relieve stress on skin beauty and mind and body, and to clear the head.

The cause of acne has not yet been clearly identified, except for genetic and environmental factors. It is known from previous studies that three major causes are directly involved in the development of acne. The three main causes are sebum that is first secreted in excess, the second cause is the blockage of pores due to hyperkeratininzation of hair follicles, and the third cause is propionibacterium acnes . Acne bacteria are described as an important factor in worsening the development of acne. Acne bacteria that reside in the hair follicles form a pore wall irritant, and the microvesicles gradually increase in size as the excess secretion of sebum continues to develop into the cotton follicles, resulting in thinning of the hair follicle wall. The material stimulates the thinned follicle wall, which gradually exacerbates the scalp into inflammatory lesions. Conventionally, as a method for treating or alleviating acne, a method of using female hormones such as estrogen, which is a male hormone inhibitor, or an antimicrobial agent has been used to suppress sebum hypersecretion. In recent years, 5α-reductase inhibitors have been used to

A method of suppressing sebum hypersecretion has been proposed. However, the method of using female hormone has high risk of skin inflammation as a side effect during the treatment of acne, and there are many reports of side effects caused by long-term administration of female hormone. However, only a small amount of prescription is carefully used under the observation of a doctor. In addition, typical antibacterial agents for acne bacteria are Resorcinol, sulfur and benzoyl peroxide, tetracycline,

Erythromycin (Erythromycin) or macrocycline, but the use of such antimicrobial treatment has shown some results in the treatment of acne, but has a number of problems in terms of the occurrence of antibiotic-specific side effects. For example, Retinoic, an antimicrobial substance

Acids and benzoyl peroxides are highly susceptible to the occurrence of skin irritation and contact dermatitis, and antibiotics containing tetracycline may cause side effects due to the appearance of resistant bacteria and photosensitization.

Meanwhile, iris (Acorus calamusvar. Angustatus) belongs to Chunnamseong (Araceae), and mainly lives in ponds or ditches in Korea, Japan, and China, and has aromatic substances throughout the roots, leaves, and roots. Silver rhizome

It is known to contain 3 to 5% in Rhizome and 0.1 to 0.5% in Leaf. The main components of essential oils are 70 to 80% of arone, and other carameones are included, and vitamin C, tannin, alkaloids, and acotin are generally rich. (Bo Bo-seop and Shin Min-kyo, Hyang-Yaksa Dictionary, Young-lim History, pp278-280,1998).

The present invention solves the above problems and has been devised by the above necessity, and an object of the present invention is to provide a composition which is harmless to the skin and the environment and has a bacterial inhibitory activity.

In order to achieve the above object, the present invention provides an antimicrobial composition against pathogenic bacteria comprising at least one compound selected from the group consisting of methyl isoeugenol and cyclohexanone as an active ingredient.

In one embodiment of the present invention, the pathogenic bacteria include all Gram-negative bacteria, Gram-positive bacteria and the like, in particular, it is preferable that the bacteria selected from Escherichia coli , Salmonella typhimurium , and Staphylococcus aureus , but is not limited thereto.

In addition, in one embodiment of the present invention, the pathogenic bacteria are preferably Propionibacterium acne , and the pathogenic bacteria include fungi and the like, but in the present invention, Candida albican is preferably but not limited thereto.

In one embodiment of the present invention, the methyl isoeugenol and cyclohexanone may be used either from a calamus oil or commercially available.

The present invention also provides a cosmetic composition comprising at least one compound selected from the group consisting of methyl isoeugenol and cyclohexanone as an active ingredient.

In the present invention, the iris oil for obtaining the methyl isoeugenol and cyclohexanone is characterized in that it is extracted by a conventional extraction method for extracting essential oil components, including steam distillation.

The cosmetic composition also includes a formulation of a flexible lotion (skin), cream, emulsion (lotion), essence, nutrients, cleaning solution, packs, cosmetics and foundation.

The iris oil for obtaining methyl isoeugenol and cyclohexanone contained in the cosmetic composition of the present invention may be obtained by various extraction methods, and in particular, by steam distillation or direct extraction using a polar solvent.

Specifically, root and root of the irises of the present invention are harvested before the leaves in early March and dried in the shade of fresh wind for 10 to 20 days, and iris leaves twice in mid-May and late July Collect the leaves grown over 10 days to 20 days, preferably 10 days to 15 days, dried in fresh windy shades and ground with a crusher, which is then steamed from below using an essential oil extractor. The fragrance oil component which is blown off and boiled with water vapor is cooled to obtain the iris oil of the present invention, from which methyl isoeugenol and cyclohexanone can be obtained.

In addition, the iris oil for obtaining the methyl isoeugenol and cyclohexanone of the present invention is a polar solvent such as methanol, ethanol, acetone or a mixed solvent of 5 to 10 times by weight of the root diameter, root and leaf of the dried iris. It can also be obtained by distillation extraction for 1 to 14 days using a polar solvent direct extraction method for 1 to 5 hours warm extraction.

The present invention provides a cosmetic composition for acne skin improvement comprising methyl isoeugenol and cyclohexanone.

The composition containing the methyl isoeugenol and cyclohexanone of the present invention as an active ingredient can be used in a variety of cosmetics and face washes for acne skin improvement effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins are microbial transformation, microorganisms

It can be obtained by conventional methods such as purification from enzyme culture, enzyme method or chemical synthesis method.

The oil-soluble vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-α-tocopherol, d-α-tocopherol, d-δ-tocopherol) and the like. Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-α-tocopherol acetate, dl-α-tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl nicotinic acid) Alcohols, pantotenylethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention.

Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzyme methods or chemical synthesis methods, or can be purified and used from natural products such as dermis such as pigs and cows and silk fibers of silkworms.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, purified from these seaweed extracts, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cold depressants, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, linoleic acid

Lofil, ethyl oleate, isocetyl myristin, isostearyl myristin, isostearyl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacinate, diisopropyl adipic acid, neopentanoic acid Isoalkyl, tri (capryl, capric acid) glyceryl, tri

2-methylhexanoic acid trimethylolpropane, triisostearic acid trimethylolpropane, tetra 2-ethylhexanoic acid penta erythritol, cetyl acetate, decyl laurate, hexyl laurate, myristic acid decyl, beforehand Myristenine Styrene, Myristinate Cetyl, Stearyl Stearate, Decyl Oleate, Ricinooleate Cetyl, Isostearyl Laurate, Isotridecyl Palmitate, Isoacetyl Palmitate, Octyl Stearate, Isoacetyl Stearate Isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isostearic acid 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, dioleate Ethylene glycol, dipropylene propylene glycol, di (caprylic capric acid) propylene glycol, dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tree Glyceryl Frylate, Glyceryl Triundecylate, Glyceryl Triisopalmitate, Glyceryl Triisostearate, Octyldodecyl Neopentanoate, Isostearyl Octane, Octyl Isononate, Hexyldecyl Neodecanoate, Neodecane Octyldodecyl, isostearic isocetyl, isostearic acid isostearyl, isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerol isostearic acid ester, citrate triisocetyl, triisoalkyl citrate, triisooctyl citrate , Lauryl lactate, myristyl lactate, cetyl lactate, octyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, sheet

Trioctyl phosphate, diisostearyl malic acid, 2-ethylhexyl hydroxystearic acid, diethyl ethyl succinic acid, diethyl butyl succinate, diisobutyl adipic acid, diisopropyl sebacinate, dioctyl sebacate, cholesteryl stearate, iso Cholesteryl stearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phystesteryl isostearic acid, phystesteric oleate, 12-stealoyl hydroxystearate isocetyl, Ester system, such as 12-stealoyl hydroxy stearate stearyl and 12-steloyl hydroxy stearate isostearyl, etc. are mentioned.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methylsteoxysiloxane copolymer, and alkyl-modified silicone. Oil, amino modified silicone oil, etc. are mentioned.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As the water-soluble low molecular moisturizer, serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization degree n = 2 or more), polypropylene glycol (Polymerization degree n = 2 or more), poly

Glycerin (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned. Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

As the water-soluble polymer, carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, dextrin

Etc. can be mentioned.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxystearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE · POP (Polyoxyethylene Polyoxypropylene) Copolymer, POE / POP Alkyl Ether, Polyether Modified Silicone, Laurin Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphates, Alkloylalkylta

Urina salt, N-acyl amino acid salt, POE alkyl ether carboxylate salt, alkyl sulfosuccinate salt, sodium alkyl sulfo acetate, acyl-hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. are mentioned.

Examples of cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyl trimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzyl ammonium bromide and behenyltrimethylammonium chloride.

Monium, benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropyl stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of amphoteric surfactants include carboxybetaine type, amide betatype, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengal, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, coloramine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium;

Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-partoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) -cured fatty acid acyl arginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono 2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, urocanine Acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzophenone, Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2'-ethylhexyl-1'-oxy) -1, 3,5-triazine, 2- (2-hydroxy Ci-5-methylphenyl) benzotriazole and the like.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, Sodium Mononitroguicol,

Undecylenic acid etc. are mentioned.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium fmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like. Higher alcohols such as cetyl alcohol are mentioned. .

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to gross weight, More Preferably it is 0.01-3 weight% mix.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the iris oil as an active ingredient, for example, conventional auxiliaries and carriers such as stabilizers, solubilizers, vitamins, pigments and flavorings. It includes.

Cosmetic compositions for improving acne skin of the present invention can be prepared in any formulation commonly prepared in the art, for example, emulsions, creams, lotions, packs, foundations, lotions, essences and the like.

Specific examples of the cosmetic of the present invention include a flexible lotion, nourishing lotion, nourishing cream, cleansing cream, cleansing foam, cleansing cream, cleansing milk, cleansing lotion, massage cream, cold cream, moisturizing cream, pack, aftershave cream, sunburn prevention cream, Suntan oil, a foundation, etc. are mentioned.

When the formulation of the present invention is a paste, cream or gel, animal fibers, plant fibers, waxes, paraffins, starches, trakants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier component may be fatty alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described.

The present invention analyzed the oil of the irises by GC-MS, and confirmed that the main components are methyl isoeugenol (41.5%) and cyclohexanone (21.3%), and then the individual components were identified as five kinds of bacteria. And antimicrobial testing of one type of yeast to see if it works. These results show that methyl isoeugenol or cyclohexanone can be used as a pharmaceutical or cosmetic component.

As described above, the composition of the present invention can be used as an environmentally friendly antibacterial and antifungal agent because it is composed of 100% natural ingredients using methyl isoeugenol and cyclohexanone harmless to the human body extracted from plants.

1 is a list of the main components of the oil derived from the root stem of the iris,
Figure 2 shows the results of experiments on the antimicrobial effect of several kinds of experimental bacteria of methyl isoeugenol and cyclohexanone.

Hereinafter, the present invention will be described in more detail with reference to non-limiting examples.

However, the following Preparation Examples, Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Comparative Examples, Examples and Experimental Examples.

Manufacturing example  1: iris oil ( oil Manufacturing

The roots and roots of irises were harvested from the Sanmul Botanical Garden of Dongyang-myeon, Dongyang-myeon, Chungju-si, before the leaves sprouted in early March, and dried and used in the shade of the cool wind for a full time. The leaves are taken from Dongyang-myeon Sanmulification Botanical Garden, Dongyang-myeon, Chungju, and then dried in a cool windy area for a full time, and then powdered with a crusher and powdered by using an essential oil extractor (Dongeco Works, Japan). After being put into the extraction apparatus, steam was blown from below to cool and separate the fragrance oil component boiled together with the steam to obtain calamus root oil (1.5-2ml / 500g) and calamus leaf oil (300ul-500ul / 400g).

Example  One: GC - MS  analysis

Analysis of the oil obtained from the above preparation was carried out using Hewlett Packard 6890 GC equipped with HP5-MS (30m x 0.25mm, film thickness 0.25um) capillary column and mass spectrometer (Beckman DU-530, USA) . An electron ionization system with an ionization energy of 70 eV was used for GC-MS detection. At a flow rate of 1 ml / min, helium was the carrier gas, and the injector and detector were set at 220 ° C. and 290 ° C., respectively.

The column temperature was initially increased to 50 ° C., then gradually increased to 150 ° C. at a rate of 3 ° C./min, held for 10 minutes and finally increased from 10 ° C./min to 250 ° C. 1.0 μl of diluted sample (1/10 in diethyl ether) was injected into the autosampler.

Identification of the components was performed by comparing their mass spectra with library data of the GC-MS system.

Experimental Example  1: Antimicrobial Activity Assay

The strain used in the experiment of the present invention is Gram-positive bacteria ( Staphylococcus aureus And Bacillus subtilis), Gram-negative bacteria ( E. coli , Salmonella typhimurium And P. acne ) And yeast ( C. albican ) was used .

Microbial strains were P. acne (GAM medium and anaerobic conditions) and C. albican (25 ° C. in Sabouraud agar) were incubated overnight at 37 ° C. in Mueller-Hilton agar.

The antimicrobial activity of the present invention was measured using the disk diffusion method.

A 6 mm autoclaved Whatman No. 1 filter paper disc was used.

Sterile filter paper discs were allowed to absorb into 30 ul of sample. 40% of commercial methyl isoeugenol (Spectrum Chemical, USA) and 20% of commercial cyclohexanone (Sigma-Aldrich, USA) was used for the antimicrobial activity test. tetracycline (Sigma-Aldrich, USA) was used as a positive antimicrobial control. Disks containing the sample were placed on agar plates previously inoculated with each microbial strain.

We identified about 80 components from the iris essential oil by GC-MS analysis. Methyl isoeugenol was the most abundant component (41.5%) followed by cyclohexanone (21.3%). These two components accounted for over 60% of the total.

1 shows the main constituents, which comprise at least 1% of the total amount.

2 shows antibiotic activity against five bacteria and one yeast of methyl isoeugenol and cyclohexanone. As can be seen in Figure 2, methyl isoeugenol and cyclohexanone showed a similar anti-bioactivity pattern except for B. subtilis .

Claims (3)

Acne relief cosmetic composition having an antibacterial effect on Propionibacterium acne containing a cyclohexanone compound as an active ingredient.
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