KR101038034B1 - Composition for promoting growth of turf and preventing turf diseases with antifungal microorganisms, and method for controlling turf by using the same - Google Patents

Abstract

The present invention relates to a composition for promoting growth and disease control of grass containing antagonistic microorganisms and a grass care method using the same, and more specifically, to the effect of feeding or fertilizer of microorganisms on soil improver adsorbing antagonistic microorganisms effective in grass disease. By adding additional natural products, and mixed with the grass soiling sand and treated during aeration and soiling of the grass, the composition for promoting growth and pest control of the antagonistic microorganism-containing grass to obtain high grass disease control effect and grass growth promoting effect and the same It relates to a lawn care method used.

Antagonist microorganism, mineral for soil improvement, zeolite, vermiculite, rice bran, aeration work, topsoil, TAC treatment

Description

Composition for promoting growth of turf and preventing turf diseases with antifungal microorganisms, and method for controlling turf by using the same}

The present invention relates to a composition for promoting growth and disease control of grass containing antagonistic microorganisms and a grass care method using the same, and more specifically, to the effect of feeding or fertilizer of microorganisms on soil improver adsorbing antagonistic microorganisms effective in grass disease. By adding additional natural products, and mixed with the grass soiling sand and treated during aeration and soiling of the grass, the composition for promoting growth and pest control of the antagonistic microorganism-containing grass to obtain high grass disease control effect and grass growth promoting effect and the same It relates to a lawn care method used.

Unlike other crops, golf course turf is difficult to completely renew soil after it is once created, and intensive management is necessary to maintain optimal conditions at all times due to the nature of golf course operations. Currently, lawn care in golf courses is dependent on chemical control methods using organic synthetic pesticides due to lack of manpower, convenience, and lack of alternative control means. Since pesticides must be sprayed repeatedly to prevent disease, there is a high risk of causing side effects such as pesticide toxicity, resistant pathogen expression, and soil and grass pesticide accumulation.

As a way to overcome the problems of chemical pesticides, biological control is being used in many crops including grass. Biological control refers to controlling pathogens, pests, and weeds that are harmful to humans by using antagonism or parasitic relationships between microorganisms, insects, plants and other organisms. Among them, research to prevent pests in many crops including grass using antagonistic microorganisms selected from soil or plants has been conducted using many kinds of bacteria and fungi since the early 1900s. Most of the biological control agents reported to date are Agrobacterium sp.), Bacillus sp.), Pseudomonas sp., Streptomyces sp. and other fungi such as Gliocladium virens and Trichoderma sp. .

In particular, according to Korean Patent No. 294023 "Bacteria for crop pest control, microorganisms containing the same and uses thereof", Bacillus subtilis ( Bacillus subtilis ) GB-0365 has been shown to have a high effect on the control of Brown patch, Large patch, and other grass diseases caused by Rhizoctonia spp. . In addition, Trichoderma harzianum ) A technique for controlling Pythium blight using ABGC-95 has been disclosed (" Trichoderma Biological Control of Pythium Blight on Golf Greens Using harzianum ABGC-95 ", The Korean Turfgrass Society, 1999. 223-234pp).

Long-term use of golf course turf can lead to turf pressure, soil solidification, ground exposure to turf roots, increased turf stress, and umbilical accumulation, resulting in poor soil ecosystems and growth. In addition, since most grass diseases are soil diseases caused by pathogens in the soil, most of the pesticides used for controlling pests are ineffective and disappear. In order to solve this problem, golf managers regularly try to improve the soil ecosystem and turf cultivation environment through aberrations such as aeration, soil, reel, vertical, etc. There are many. Zeolite, peat moss, pearlite, olivine, etc. are widely used. These soil modifiers have effects such as soil drainage, bovine power, permeability, pH control, and grain formation.

However, biological control methods and soil modifiers using antagonistic microorganisms have many disadvantages in terms of effect reproducibility, ease of use, and user perception, and have limited disadvantages in terms of cost. In order to solve this problem, there is an urgent need for research and product development that can take advantage of soil improvers and antagonistic microorganisms and increase their effectiveness.

Therefore, an object of the present invention is to prevent the reduction of the effect due to the influence of the external environment, which is a problem of the existing antagonist microbial agent by mixing and treating the antagonist microbial agent, increase the bottle control effect, easy formulation for machine work It is to provide a composition for promoting growth and pest control of antagonistic microorganism-containing grass having high ease of use.

Another object of the present invention is to provide a method for managing grass using the composition for promoting growth and disease control of the antagonistic microorganism-containing grass.

In order to achieve the above object, the present invention

Antagonistic microbial agents including antagonistic microorganisms and carriers for turfgrass control; And

It provides a composition for promoting growth and pest control of antagonistic microorganisms containing grass including the clay.

The invention also

Adsorbing antagonistic microorganisms for controlling turfgrass on a carrier;

Preparing a antagonist microbial agent by mixing a food source of antagonist microorganisms or a nutrient source of grass to the carrier; And

It provides a method for producing a composition for promoting growth and pest control of the antagonist microbial turf comprising the step of mixing the antagonist microbial agent with the clay.

The present invention also provides a lawn care method comprising the step of treating the composition for promoting growth and disease control of grass of the present invention during aeration and soiling of the grass.

The present invention by using a mixture of soil improvers and natural products adsorbing antagonism microorganisms and mixed with the clay soil during aeration and soiling of the golf course to suppress the occurrence of grass disease, promote the growth of grass, grass planting It has the effect of improving the soil drainage, bobbin power, permeability. This can reduce the microbial loss caused by the external environment, which is a disadvantage of the existing antagonistic microorganisms, and can increase the influence on the pathogen by directly treating the soil, which is the habitat of the soil pathogen causing the grass disease.

In addition, the interaction with the soil improving agent, a carrier of the antagonist microorganisms shows a higher viability and can be equivalently effective even if the throughput of the antagonist microorganisms significantly reduced.

EMBODIMENT OF THE INVENTION Hereinafter, the structure of this invention is demonstrated concretely.

The present invention

Antagonistic microbial agents including antagonistic microorganisms and carriers for turfgrass control; And

The present invention relates to a composition for promoting growth and disease prevention of antagonistic microorganism-containing turf including a clay soil.

The composition for promoting growth and disease control of grasses containing antagonistic microorganisms of the present invention controls major diseases occurring in grass while overcoming disadvantages such as microbial loss and weakening effect by the external environment of the existing antagonistic microorganisms and grows grass. It is an antagonistic microbial agent that is adsorbed to minerals with soil improving effect to improve acceleration, soil drainage, fertility, and permeability, and is a food for microorganisms, and mixes natural materials with fertilizer on grass.

In addition, by increasing the convenience of the work by treating the composition for promoting growth and disease control of the antagonist-containing grass with the excavation company during aeration and vomiting of the golf course, it is possible to increase the control effect of soil pathogens by treating the antagonistic microorganisms directly to the soil. There is an advantage.

Lawn bottles that can be controlled by the composition for promoting growth and disease control of grass antagonism-containing microorganisms of the present invention is Brown patch, Laytonian blight (Large patch), Dollar spot or Pisium blight (Pythium blight).

The antagonistic microorganisms are preferably Bacillus microorganisms. More preferably, Bacillus subtilis ( Bacillus subtilis ) GB-0365 KFCC 11071, Bacillus subtilis ) EW42-1 KCCM 10565, or their cultures, may be used alone or in combination. In particular, when used in combination, it is preferable that these strains are not mutually antagonistic.

The antagonistic microorganisms can be adsorbed onto the carrier in a lyophilized state, or the culture medium can be adsorbed according to a conventional method, so that the state of the microorganisms is not particularly limited. More preferably, the culture solution itself is adsorbed.

In addition, the carrier is preferably a soil improver that can maintain stability over time upon adsorption of antagonistic microorganisms, and improve the physical properties of the soil when used in the golf course grass planting area.

The carrier is not particularly limited, but in view of the effect as a soil improving agent and maintaining the density of the antagonist microorganisms, the carrier has a porous structure so that the antagonist microorganisms are easily adsorbed, and minerals capable of proliferating the microorganisms using nutrients in the culture medium in the pores. good. Preferably, zeolite, calcined zeolite, vermiculite, vermiculite, isolite, pearlite or the like may be used alone or in combination of two or more thereof. More preferably, calcined zeolite is used.

The particle size of the carrier is selected from 0.5 to 2mm range so that it can be mixed with the clay, it is preferable that the particle size is 0.5% to 1mm is more than 90% composition. Particle size of the carrier can be adjusted differently depending on the application area, when applied to the fairway instead of putting green, it is preferable to adjust the roughness and the thickness almost the same.

In addition, the antagonist microbial agent may further include a food source of the antagonist microorganisms or a nutrient source of the grass, for example, a natural product having a fertilizer effect.

As a natural product, other agricultural by-products (oil meal), such as rice bran, corn starch, bean curd, or bran, may be used alone or in combination of two or more.

The antagonist microbial agent of the present invention is preferably prepared by mixing the antagonist microorganism, the carrier and the natural product in a ratio of 0.5 to 1: 8 to 12: 0 to 1.5 (v: wt: wt). In addition, the antagonist microbial agent preferably contains 1 × 10 ⁷ cfu / ml to ⁹ × 10 ⁹ cfu / ml of the antagonistic microorganism. More preferably, the mixing ratio of the antagonist microorganism, carrier and natural product is 1: 9: 1 (v: wt: wt).

In addition, the antagonist microbial agent is preferably maintained at a final moisture content of 6 to 9%, because when the moisture exceeds the above range, decay or solidification occurs due to the penetration of external bacteria.

In addition, it is preferable that the particle size of the excavated sand is easily mixed with the soil and is 0.5 to 2 mm in consideration of the size of a conventional aeration hole.

In addition, it is preferable to mix the antagonist microorganisms and the clay in a ratio of 1: 2 to 50 in consideration of the clay height of the putting green. More preferably, they are mixed in the ratio of 1: 5-30. Mixing of the antagonist microorganisms and the excavated sand may use a heavy equipment such as a conventional stirrer or forklift, but is not particularly limited thereto.

The invention also

Adsorbing antagonistic microorganisms for controlling turfgrass on a carrier;

Preparing a antagonist microbial agent by mixing a food source of antagonist microorganisms or a nutrient source of grass to the carrier; And

The present invention relates to a method for promoting growth of antagonistic microorganisms-containing grass comprising the step of mixing the antagonist microorganisms with the clay.

In order to prepare the composition for promoting growth and disease control of grass of the present invention, turfgrass, for example, brown leaf blight, coin blight, pistil blight, etc. can be controlled, and antagonistic microorganisms having stability over time are selected. .

Next, a carrier that can maintain stability over time upon adsorption of the antagonistic microorganisms and improve the physical properties of the soil is selected as a soil improving agent. The carrier is a porous structure of the mineral, and the adsorption and propagation of the antagonist microorganisms are possible by using nutrients in the culture medium in the pores. The carrier selected is preferably adjusted to have a particle size of 0.5 to 2 mm, but 0.5 to 1 mm 90% or more to enable mixing with the clay.

Next, antagonist microorganisms are prepared by mixing antagonist microorganisms and natural products corresponding to nutrient sources of turf, for example, rice bran and the antagonist microorganisms and carriers selected above. The mixing ratio of the antagonist microorganism, carrier and mixture is preferably 0.5 to 1: 8 to 12: 0 to 1.5 (v: wt: wt). In addition, the final moisture content of the antagonist microbial agent is preferably maintained at 6 to 9%, because it can suppress the rot and freezing caused by the invasion of foreign bacteria.

Next, by mixing the antagonist microbial agent and the clay in a ratio of 1: 2 to 50 to prepare a composition for promoting growth and pest control of grass of the present invention.

The present invention also relates to a lawn care method comprising the step of treating the composition for promoting the growth and pest control of the antagonist microorganism-containing grass of the present invention during aeration and soiling of the grass.

When the turfgrass discharging work is preferably aeration and discharging work, the composition for promoting growth and pest control of the antagonist microorganism-containing turf mixed with the antagonist microbial agent of the present invention is treated.

The composition is treated to the turf according to the TAC (Topdressing Aeration Core) treatment method that can minimize the impact from the external environment by treating the hole generated during aeration.

The height of the putting green of the putting green is preferably 1.5 to 2 mm, and preferably 3 to 4 mm after the aeration operation. For this purpose, the mixing ratio of the antagonist microorganisms and the excreted sand is preferably 1: 9 to 49. Therefore, the composition is preferably treated 1.4 to 5.0 kg per 1 m ² of grass.

The following examples are only for illustrating the present invention, and it is apparent to those skilled in the art that these specific techniques are only preferred embodiments, and thus the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Example 1 Preparation of Antagonist Microbial Formulations

An antagonist microorganism was prepared by selecting antagonistic microorganisms effective for the major diseases of turf and selecting a carrier which can enhance the effects of these antagonist microorganisms.

(Selection of antagonistic microorganisms)

Most of the diseases occurring in the grass are caused by soil pathogens present in the grass umbilical layer. The disease is more severe when several pathogens affect the grass together than when invading pathogens alone. For this reason, it may be more effective to mix antagonistic microorganisms that are effective against pathogens expected to exist in the same area than to treat antagonistic microorganisms that are effective in controlling the pathogen.

Therefore, Green Biotech's antagonist Trichoderma spp., Bacillus spp. And Paenibacillus are effective against the major diseases of grass. spp.) and the like to check the stability over time. In order to check the stability over time, all the antagonistic microorganisms were mixed in the same ratio, and after 48 hours, the viable cell count (cfu / ml) was measured. The experimental results are shown in Table 1.

As shown in Table 1, in the case of the two antagonistic microorganisms of A and B, the density of the bacteria hardly decreased, but the other antagonistic microorganisms showed a sharp decrease in the bacterial density.

From this, Bacillus strains, A and B were selected. The two strains of cool - season grass Creeping Bent Grass (Creeping bentgrass) representative grass and brown leaf blight disease (Brown patch), Bacillus subtilis (Bacillus in which this effect occurs subtilis ) GB-0365 KFCC11071, Bacillus subtilis ) EW42-1 KCCM10565, which is suitable for the purpose of this experiment as a strain that has an even effect on major turfgrasses such as Dollar spot and Pythium blight in addition to brown leaf blight.

Using the above strain, a control experiment for brown leaf blight was carried out in creeping bentgrass, which is a green grass in putting practice green of Seoul CC. This test site was a natural occurrence of brown leaf blight, which was conducted at the time of onset of brown leaf blight around August 2006. Three experiments were carried out with an area of 2 × 2 m ^{2 per} test zone, which was diluted 200 times with water and treated with 1 L / m ² . Antagonistic microorganisms were treated three times at weekly intervals, and after 7 days of the final treatment, the control value was calculated based on the area area of the disease, and the efficacy was evaluated. However, the mixing ratio of the antagonistic microorganisms was 1: 1 (V: V) and was treated within 48 hours of dilution. The results are shown in Table 2.

Control value = (average incidence area ratio of untreated control-average incidence area of experimental group) / average incidence area ratio of untreated control × 100 (%)

As shown in Table 2, the antagonistic microorganisms exhibited more than 60% control value when treated with the antagonistic microorganisms compared to the untreated control, and when mixed with the antagonistic microorganisms, the control agent showed about 95% higher control value.

These antagonistic microorganisms were used in the preparation of new antagonist microorganisms in the following experiments.

(Choice of carrier)

Carriers were selected to maintain stability over time upon adsorption of antagonistic microorganisms and to improve soil physical properties when used in grass planting areas of golf courses.

Carrier was selected based on the effect as a soil improver and maintaining the density of the antagonist microorganism, and the number of viable bacteria (cfu / g) was measured at the time of the day, 7 days, 14 days by spray adsorption of the antagonist microbial culture. The ratio of the antagonistic microorganisms and the carrier was 1:10 (v: wt), and the result was stored in a constant temperature room at 30 ° C to prevent contamination. The results are shown in Table 3.

As shown in Table 3, the calcined zeolite was found to have the highest stability over time, and was judged to be suitable as a carrier. In the case of calcined zeolite, antagonistic microorganisms were easily adsorbed as minerals of porous structure, and the results of propagation using nutrients in the culture medium in the pores were confirmed.

The selected carrier was prepared by adjusting the particle size of the clay to be 0.5 to 2.0 mm, so that 0.5 to 1.0 mm or more could be mixed with the clay.

(Agitation and adsorption of antagonistic microorganisms, carriers and natural products)

Antagonist microorganisms and carriers selected above were mixed with natural products to prepare antagonist microorganisms through stirring and adsorption.

The antagonist microbial agent was used rice bran as a food source and nutrient source of the antagonist microorganisms.

The mixing ratio of the two types of antagonistic microorganisms, carriers and rice bran selected above was adjusted to 5 × 10 ⁹ (cfu / ml) when producing 1.1 ton, and 1: 9: 1 (V: Wt: Wt).

After manufacture, the final product had a water content of about 7%.

Example 2 Investigation of the growth promotion and disease control effect of the grass according to the composition treatment of the present invention

Existing golf course antagonist microorganisms are mostly in the form of hydration, and the spray itself is impossible due to the disappearance of ultraviolet rays between 12 and 2 o'clock in the middle of the day, and it has to be applied after 4 pm. This is very inconvenient for managers in the work environment and practically impossible to use, which is a big reason why antagonist microorganisms cannot be used on site. In addition, even if the antagonism microorganisms treated in a timely manner, most of the situation does not reach the umbilical layer present in soil pathogens.

Accordingly, the present invention is to process the antagonist microbial agent mixed with the clay to the grass during the work, when the aeration and acupuncture is made, in September 2007 Paju Seoul, Gyeonggi-do, Republic of Korea putting green grass in the creeping bent grass The experiments were conducted on the control of brown leaf blight and recovery of grass after aeration.

In this experiment conducted at the place where brown leaf blight was to occur, the unit area per experimental section was 4m ² , and the experiment was performed with 3 replicates per experimental section. The aeration operation was carried out 160 holes per 1 m ² with a diameter of 2 cm, depth 10 cm, 10 kg per m ² was treated with a mixed soil antagonist microbial agent mixed in each ratio using a stirrer. The mixed soil antagonist microorganism was treated according to the TAC (Topdressing Aeration Core) treatment method, the height of the soil was about 1mm, and after the aeration operation was 3.5mm. After the treatment, the disease incidence was examined at 7 days intervals, and after 21 days, the control value was calculated based on the area of disease occurrence in the control group of Batosa alone, and the efficacy was evaluated.

In order to evaluate the growth of shoots, the period of filling the holes generated during aeration was measured, and the evaluation was based on the coverage (%) 7 days after the treatment. The management of this study site was carried out in the same manner as the management of the golf course, and the experimental area was not treated with chemical pesticides and fertilizer other than antagonistic microbial agents. The experimental results are shown in Table 4.

As shown in Table 4, when the product diluted at 1:19 ratio was considered to be effective and economical, the most suitable dilution ratio was shown, and the control value was 100% higher than that of the soil treatment company. And it was found. The coating after 7 days was also very high (95%) even without fertilizer, indirectly verifying the shoot production and fertilizer effect.

Claims (10)

Antagonistic microbial agents containing mixed antagonistic microorganisms and calcined zeolites of Bacillus subtilis GB-0365 KFCC11071 and Bacillus subtilis EW42-1 KCCM10565; And Include excreta, The antagonist microbial agent and the soil release composition for promoting growth and pest control of the antagonist microorganisms containing grass, characterized in that the mixing ratio of 1: 2 to 50. The method of claim 1, Turfgrass is at least one selected from the group consisting of Brown patch, Large patch, Large spot, Dollar spot and Pythium blight Composition for promoting growth and disease prevention. delete delete The method of claim 1, Antagonist microbial agent is a composition for promoting growth and pest control of the antagonist microorganism-containing grass further comprises a natural product that is a food source or nutrient source of the antagonist microorganisms. The method of claim 5, The natural product is a composition for promoting growth and pest control of antagonistic microorganism-containing grass, characterized in that at least one kind of oil foil selected from the group consisting of rice bran, corn starch, sesame and bran. The method of claim 5, Antagonist microorganisms are prepared by mixing antagonist microorganisms, carriers and natural products at a ratio of 0.5-2: 8-12: 0-1.5 (v: wt: wt), wherein the mixture contains 1 × 10 ⁷ cfu / ml A composition for promoting growth and pest control of antagonistic microorganisms-containing grass, characterized by being contained at ˜9 × 10 ⁹ cfu / ml. delete Mixed antagonistic microorganisms of Bacillus subtilis GB-0365 KFCC11071 and Bacillus subtilis EW42-1 KCCM10565 are adsorbed to calcined zeolites and antagonistic microorganisms are added by adding food sources of antagonists or nutrient sources of grass Preparing a formulation; And Method of producing a composition for promoting growth and pest control of the antagonist microbial turf comprising the step of mixing the antagonist microbial agent in a weight ratio of 1: 2 to 50. Lawn care method comprising the step of treating the composition for promoting the growth and pest control of the antagonist microorganism-containing grass according to claim 1 during the aeration and soiling of the grass.