KR101026816B1 - 핵산 집단내 오류 저감 방법 - Google Patents

핵산 집단내 오류 저감 방법 Download PDF

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KR101026816B1
KR101026816B1 KR1020047013498A KR20047013498A KR101026816B1 KR 101026816 B1 KR101026816 B1 KR 101026816B1 KR 1020047013498 A KR1020047013498 A KR 1020047013498A KR 20047013498 A KR20047013498 A KR 20047013498A KR 101026816 B1 KR101026816 B1 KR 101026816B1
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dna
double
strand
probe
sequence
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KR20040088562A (ko
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서스맨마이클알
세리나프란시스코
벨쇼피터제이
케이슨제임스에이치
리치몬드캐스린
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위스콘신 얼럼나이 리서어치 화운데이션
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Paper (AREA)
  • Saccharide Compounds (AREA)
KR1020047013498A 2002-02-28 2003-02-28 핵산 집단내 오류 저감 방법 Expired - Fee Related KR101026816B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36056302P 2002-02-28 2002-02-28
US60/360,563 2002-02-28
PCT/US2003/006500 WO2003072832A1 (en) 2002-02-28 2003-02-28 Method of error reduction in nucleic acid populations

Publications (2)

Publication Number Publication Date
KR20040088562A KR20040088562A (ko) 2004-10-16
KR101026816B1 true KR101026816B1 (ko) 2011-04-04

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KR1020047013498A Expired - Fee Related KR101026816B1 (ko) 2002-02-28 2003-02-28 핵산 집단내 오류 저감 방법

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Country Link
US (2) US7303872B2 (https=)
EP (1) EP1483409B1 (https=)
JP (1) JP4455061B2 (https=)
KR (1) KR101026816B1 (https=)
AT (1) ATE428805T1 (https=)
AU (1) AU2003213696B2 (https=)
CA (1) CA2477519C (https=)
DE (1) DE60327183D1 (https=)
IL (2) IL163788A0 (https=)
IS (1) IS7430A (https=)
MX (1) MXPA04008373A (https=)
WO (1) WO2003072832A1 (https=)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE403013T1 (de) * 2001-05-18 2008-08-15 Wisconsin Alumni Res Found Verfahren zur synthese von dna-sequenzen die photolabile linker verwenden
CA2477519C (en) * 2002-02-28 2013-01-22 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US8133670B2 (en) 2003-06-13 2012-03-13 Cold Spring Harbor Laboratory Method for making populations of defined nucleic acid molecules
SG110153A1 (en) * 2003-09-22 2005-04-28 Agency Science Tech & Res Device and method of detecting mutations and polymorphisms in dna
CA2558749A1 (en) * 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
EP1574570A1 (en) * 2004-03-12 2005-09-14 Universität Regensburg Process for reducing the number of mismatches in double stranded polynucleotides
ATE474936T1 (de) * 2004-06-09 2010-08-15 Wisconsin Alumni Res Found Schnelle synthese von oligonukleotiden
EP1781786B1 (en) * 2004-08-27 2011-01-26 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
EP1812598A1 (en) * 2004-10-18 2007-08-01 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US7722824B2 (en) 2005-08-02 2010-05-25 Wisconsin Alumni Research Foundation Synthesis of arrays of oligonucleotides and other chain molecules
US20070196834A1 (en) * 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
EP2504449B1 (en) 2009-11-25 2016-03-23 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN111876409A (zh) 2012-04-24 2020-11-03 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
AU2013280661A1 (en) 2012-06-25 2015-01-22 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
WO2014058890A1 (en) * 2012-10-08 2014-04-17 Spiral Genetics Inc. Methods and systems for identifying, from read symbol sequences, variations with respect to a reference symbol sequence

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120992A (en) * 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5376526A (en) * 1992-05-06 1994-12-27 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
WO1999006591A1 (en) 1997-07-31 1999-02-11 The Institute Of Physical And Chemical Research Methods for detecting mutation in base sequence
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
US6297010B1 (en) * 1998-01-30 2001-10-02 Genzyme Corporation Method for detecting and identifying mutations
GB9808145D0 (en) * 1998-04-17 1998-06-17 Zeneca Ltd Assay
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
EP2175021B1 (de) * 1999-02-19 2013-06-19 Synthetic Genomics, Inc. Verfahren zur Herstellung von Polymeren
ATE296310T1 (de) * 1999-03-08 2005-06-15 Metrigen Inc Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
ATE403013T1 (de) * 2001-05-18 2008-08-15 Wisconsin Alumni Res Found Verfahren zur synthese von dna-sequenzen die photolabile linker verwenden
US6673552B2 (en) * 2002-01-14 2004-01-06 Diversa Corporation Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps
CA2477519C (en) * 2002-02-28 2013-01-22 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120992A (en) * 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human

Also Published As

Publication number Publication date
JP4455061B2 (ja) 2010-04-21
IL163788A0 (en) 2005-12-18
EP1483409A4 (en) 2006-03-08
DE60327183D1 (de) 2009-05-28
EP1483409A1 (en) 2004-12-08
MXPA04008373A (es) 2004-11-26
AU2003213696A1 (en) 2003-09-09
CA2477519A1 (en) 2003-09-04
JP2005518799A (ja) 2005-06-30
US20090188793A1 (en) 2009-07-30
CA2477519C (en) 2013-01-22
IS7430A (is) 2004-08-30
US20040132029A1 (en) 2004-07-08
IL163788A (en) 2010-04-29
KR20040088562A (ko) 2004-10-16
AU2003213696B2 (en) 2008-01-24
EP1483409B1 (en) 2009-04-15
US7303872B2 (en) 2007-12-04
WO2003072832A1 (en) 2003-09-04
ATE428805T1 (de) 2009-05-15

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