KR101004645B1 - Cultivation method for cauliflower mushroom - Google Patents

Abstract

The present invention relates to a method of cultivating the zinnia mushroom using coniferous wood, and more particularly, by cultivating the zinnia mushroom using the coniferous wood, rather than the conventional cultivation of the zinnia mushroom using sawdust, the rate of the active wood logs It is to increase the economic efficiency and to cultivate large quantities of eco-friendly, high-quality blossom mushrooms closest to natural products.

According to the present invention, a method of cultivating a cauliflower mushroom using coniferous wood, comprising the steps of peeling and cutting the wood of conifers, preparing singlewood, attaching a culture promoter to the singlewood, and preparing a cultured wood, and the cultured wood Sterilizing, inoculating the liquid spawn on the sterilized culture tree, culturing the hyphae on the inoculated culture tree, and growing the mycelia of the cultured culture tree. do.

Softwood, zinnia, log, liquid spawn

Description

Cultivation method for cauliflower mushroom

The present invention relates to a method of cultivating the zinnia mushroom using coniferous wood, and more particularly, by cultivating the zinnia mushroom using the coniferous wood, rather than the conventional cultivation of the zinnia mushroom using sawdust, the rate of the active wood logs It is to increase the economic efficiency and to cultivate large quantities of eco-friendly, high-quality blossom mushrooms closest to natural products.

In general, blossom mushrooms are mainly distributed in Korea, Japan, China, North America, Europe, Australia, etc., and occur in summer and autumn in dead stumps of coniferous pines, larch, and firs.

This bacterium is a brown fusiform fungus that is the root of conifers such as larch in the natural ecosystem. The blossom mushroom is an antimicrobial action by Sprasol-1 or Sprasol-2, polysaccharides (Polysaccharids). It is known that there is an effect of enhancing the immune system, in particular, lung cancer, colon cancer, prostate cancer. In addition, the mushroom contains 43.6 g of beta glucan per 100 g of dried, and studies on beta (1 → 3) D-glucan extracted from the zinnia mushroom were carried out with transplantation of Sacoma 180 solid liver cancer into 30 g of experimental rats. After the administration of 100 micrograms of beta (1 → 3) D-glucan extracted from thermal alkali three times for 35 days, it was found that it had a 100% cancer suppression effect and was attracting attention (中 島 三 夫, 宿 前 利). It is known to have significant effects on the immune system of humans, 抗 ガ ン の キ ノ コ ハ ナ ラ タ ケ) and has been attracting worldwide attention as it was introduced in 2007 in the journal Nature.

However, these mushrooms are difficult to artificially cultivate due to rosin components, which are presumed to be inhibitory factors, and are dependent on natural harvesting in autumn.

Therefore, there has been a continuous research on artificial cultivation of zinnia mushroom, and as a part of this, in Korea, genetic analysis was performed using wild zinnia mushrooms collected from Gwangneung, Gyeonggi-do and strains distributed in the Netherlands. Fruit cultivation has not been attempted or successful. (Kim Ji-yeon, 2000, Genetic analysis using ITS of blossom mushroom, Master's thesis, Dongguk University)

In addition, blossom mushroom is extracted by hot water, more than 90% of the active ingredient is a mushroom that can be expected to be effective only by human intake.

The above-mentioned blossom mushroom is difficult to mass-produce due to the difficult conditions for artificial cultivation. In the registered patent No. 483333, after preparing a sawdust badge by adding nutritious substances to sawdust, cultivating blossom mushrooms, this method is complicated and cumbersome. Due to the high investment of the process and artificial facilities, there was a problem that it was difficult to access business in rural farms.

In addition, in Korean Patent Application Publication No. 2002-48336, which was previously filed, it is possible to short-term cultivation by forming an optimal reservoir in the incubator by drilling a hole penetrating the cutting part of the wood without using sawdust. There was a problem in that the production rate was not good to remove the inhibition.

Literature information of the prior art

Kim, Ji-Yeon, 2000, Genetic Analysis Using ITS of Blossom Mushroom, Master's Thesis, Graduate School, Dongguk University

中 島 三 夫, 宿 前 利 郞, 抗 ガ ン の キ ノ コ ハ ナ ラ タ ケ

Domestic Patent No. 483333

Domestic Publication No. 2002-48336

Accordingly, an object of the present invention is to solve various problems caused by cultivating a blossoming mushroom using conventional sawdust, and by increasing the activity rate of the seedlings by softening and softening the logs of softwoods themselves, simplifying complicated and cumbersome processes. It minimizes the investment cost of artificial facilities and ensures the stable cultivation of eco-friendly, high-quality blossom mushrooms closest to natural products.

Method of cultivating the zinnia mushroom using the coniferous wood of the present invention for achieving the above object comprises the steps of preparing a single tree by peeling and cutting the solid wood of the coniferous tree; Sterilizing the cultured tree, inoculating the liquid spawn on the sterilized cultured tree, culturing the hyphae on the inoculated cultured tree, and growing the mycelia of the cultured cultured tree. The culture promoter is characterized in that 5-15% by weight of coniferous sawdust, 5-15% by weight of bran, 5-15% by weight of jade, 5-15% by weight of malt, 40-80% by weight of water are mixed. do.

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And after coating the heat-resistant plastic pack in the open state in the cut wood, the culture promoter is attached and the top is sealed with a plastic filter.

And the conifer is characterized in that any one of larch, pine, fir, pine, beech, buckwheat, cedar.

And sterilizing the culture tree,

First sterilizing the cultured tree at 100-105 ° C. for 30-40 hours, secondly sterilizing the first sterilized culture tree at 70-80 ° C. for 16-24 hours, and performing the second sterilization. Characterized in that it comprises a step of cooling the cultured tree to 13 ~ 15 ℃.

And culturing the mycelia on the inoculated culture tree,

It is characterized by culturing for 2 to 3 months at a temperature of 15-23 ℃ and a humidity of 70-80%.

And growing the mycelia of the cultured tree,

Put the culture tree cultured mycelium in a plastic pack filled with water and leave it for 15-20 days,

After the 15 to 20 days left to induce the formation of carbon by irradiating with carbon dioxide and light,

Inducing mushroom generation of the cultured tree induced by the primordial formation,

The mushroom generation is characterized in that it comprises the step of growing the blossom mushroom of the culture tree generated.

In addition, the step of inducing the formation of the temperature, characterized in that subjected to severe stress by irradiating the temperature deviation 15 ℃ or more, humidity 80%, carbon dioxide 600 ~ 1,000ppm and light 1,000 ~ 2,500 Lux.

And after the growth step, and harvesting the mushroom mushrooms,

It characterized in that it further comprises the step of drying the cultivated blossom mushroom.

According to the cultivation method of the flower mushroom using coniferous wood according to the present invention, unlike using conventional sawdust, by sterile and soften the coniferous wood itself to increase the rate of log of the seed spawn, simplifying the complicated and cumbersome cultivation process, By minimizing the investment cost of artificial facilities, it provides useful effects such as increasing the income of farmers by cultivating flower mushrooms, which makes it easier to create new profits of difficult rural farmers in Korea.

In addition, it provides the effect to reliably cultivate high-quality flowering mushrooms, eco-friendly, pesticide-free closest to natural products.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

First is a cultivation process of the flower mushroom according to the present invention, wood peeling and cutting (S1), culture promoter attachment (S2), sterilization (S3), liquid seed inoculation (S4), culture (S5) and growth step ( S6) is configured to include. And 2a to 2h is a photograph showing the cultivation process of the blossom mushroom according to the present invention, with reference to this will be described the present invention.

Hereinafter, each step will be described in detail.

First the wood is stripped and cut to prepare lumber (S1).

At this time, it is preferable to use conifers as the wood, and the conifers may be selected from any one of larch, pine, fir, pine, beech, buckwheat and cedar. And other than the above-mentioned wood, if the cultivation of the blossom mushroom can be used other natural wood, of course.

In addition, the wood is used to cut the diameter of 10 ~ 20cm between November and February of the next year, it is good to increase the production of zinnia mushroom.

When preparing the single timber (S1), as shown in Figure 2a, after felling the logs of the conifers, to remove the branches, knots, rough hulls of the felled logs, that is, stripping, which is a post-process plastic bag coating This is to prevent the damage of plastic packs during the operation, to facilitate the movement of wood during sterilization, and to facilitate the hyphae after spawn inoculation.

When stripping is completed as described above, the stripped wood is cut into 12-15 cm of solid wood. At this time, the size of the wood to 12 ~ 15cm to increase the production of the blossom mushroom.

When the preparation of lumber (S1) is completed, as shown in Figure 2b, by attaching a culture promoter to the prepared lumber to prepare a culture tree (S2).

The culture promoter is composed of 5 to 15% by weight of coniferous sawdust, 5 to 15% by weight of bran, 5 to 15% by weight of jade, 5 to 15% by weight of malt, and 40 to 80% by weight of water. It is preferable to set it as%, wheat bran 10 weight%, jade powder 10 weight%, malt sugar 10 weight%, and water 60weight%. The culture promoter is for promoting the cultivation of the flower mushroom, so that the culture rate of the mycelium may not be good when each component is out of the blending amount, it is to be mixed based on the above-described blending ratio.

And the culture promoter is attached to the upper end of the prepared lumber with a thickness of about 0.5 ~ 1.0cm, when the thickness is less than 0.5cm, the effect of the culture promotion does not appear sufficiently and exceeds the 1.0cm excess of the efficacy Since no increase is observed, it is preferable to apply a thickness of 0.5 to 1.0 cm in consideration of economical efficiency.

By using the culture promoter it is possible to advance the first occurrence of the mushroom about 1 month.

 The single tree (hereinafter referred to as 'culture tree') to which the culture promoter is attached is packaged with a heat-resistant vinyl pack and a plastic filter, wherein the heat-resistant vinyl pack is coated in an open state with the top open before attachment of the culture promoter. It is preferable to attach an accelerator and to seal the upper part using a plastic filter etc. And the heat-resistant plastic pack does not limit the type, but because the high temperature is applied in the post-process sterilization process is to use a heat-resistant plastic pack, the plastic filter also does not limit the type.

When the preparation of the cultured tree as described above (S2) is completed, the cultured tree is sterilized (S3).

At this time, the sterilization operation is to divide the cultured wood prepared by dividing the 3-6 pieces in a heat-resistant plastic box, and load it into the sterilization chamber. And using a high pressure steam boiler in the first 100 sterilization for 30 to 40 hours at a temperature of 100 ℃ to make a sterile state, when the sterilization temperature is less than 100 ℃ or less than 30 hours sterilization is not made enough 105 ℃ If it exceeds or exceeds 40 hours, excessive energy is consumed and it is not economical, so the first sterilization should be completed using the above conditions. In the first sterilization, sterilization is performed at atmospheric pressure without adjusting the pressure conditions.

After the first sterilization is completed, the first sterilized cultured trees are secondly sterilized at 70-80 ℃ for 16-24 hours. At this time, the second sterilization is a ripening process to soften the cultivated tree and to remove some of its own gas and rosin. Therefore, the second sterilization is carried out at 70-80 ℃ for 16-24 hours in consideration of sufficient softening and economic efficiency.

When the second sterilization is completed, cool it to 13 ～ 15 ℃ so that the inoculation of liquid spawn is possible. At this time, the cooling method is not limited.

When the sterilization (S3) is completed, take out the sterilized culture tree from the plastic box, as shown in Figure 2c and inoculates the liquid seed to the sterilized culture tree (S4). At this time, the inoculation method visually checks the cultured liquid seed and visually confirms it with a peculiar smell, and selects a non-contaminated culture container and smokes and disinfects the prepared wood. It is to inoculate. At this time, the heat-resistant plastic pack is not removed, the plastic filter is opened, the seed is inoculated with liquid, and the plastic filter is sealed again.

At this time, the liquid spawn is cultured by the following method. Hereinafter, the method will be described as an embodiment tested by the present inventors.

First, prepare a petri container for tissue culture of the collected mushrooms. Mix and sterilize 3g Malta extra, 3g yeast extra, 3g peptone 5g, 20g agar and 10g glucose in 1L of water, and put a certain amount into the petri dish in a clean room to seal the graft opening in the incubator. Incubate first for 30 days.

When the primary culture is completed, mixed and sterilized with malta extra 3g, yeast extra 3g, peptone 5g, agar 20g, glucose 20g nutrients, put it into a Erlenmeyer flask in a clean room and inject the first cultured strain, filter treatment Sutured with a stopper and incubated for 20 days in an incubator.

Then, a predetermined amount of Milta extra, yeast extra and peptone maltose is mixed in 150 l of water of pH 5, and placed in a spawn culture vessel, and autoclaved at 100 ° C. for 60 minutes and 121 ° C. for 90 minutes, and then cooled to 15 ° C. or lower to prepare a culture solution. .

The second cultured strain in the cooled culture medium is finely ground with a homogenizer (15,000 rpm) and injected in a clean room.

And the culture vessel injecting the strain is incubated for 25 to 30 days by connecting to an air line installed with an air heater, air dryer, water removal, bacteria removal filter.

The method for producing the liquid spawn is described by way of example, and in addition to the above-described method, a liquid spawn which is conventionally used can be used, and the type and the production method are not limited.

When the inoculation of the liquid spawn is completed as described above (S4), as shown in FIG. 2D, the mycelia are cultured in the inoculated culture tree (S5). At this time, the culture conditions are 15 to 23 ℃ temperature and humidity conditions of 70 to 80% to incubate for 2 to 3 months so that mycelium infested white to the lower end of the culture tree in a clean culture room. If the cultivation temperature or humidity is out of the hyphae is not easily cultivated to keep the temperature and humidity conditions, the period is also cultured for 2 to 3 months to ensure sufficient cultivation.

When the incubation is completed (S5) to remove the heat-resistant vinyl pack and plastic filter, as shown in Figure 2e, the mycelia of the cultured culture tree (S6). At this time, the growth method is the cultured wood, the heat-resistant plastic pack and the plastic filter is removed, put into a plastic pack in which water is added, the step of leaving for 15 to 20 days, and the left after 15 to 20 days carbon dioxide and Inducing primitiveness by irradiating light, inducing mushroom development of the cultivation-derived cultivated tree, and growing the blossom mushroom of the cultivated tree in which the cultivation is induced Do.

That is, in order to grow the hyphae, a suitable amount of clean water, that is, drinking water, is put in a plastic pack, preferably a polypropylene pack, and the cultured tree is placed in the plate and left for 15 to 20 days.

After being left for 15 to 20 days, irradiated with light of temperature deviation of 15 ℃ or higher, humidity 80%, carbon dioxide 600 ~ 1,000ppm, light 1,000 ~ 2,500 Lux to induce reproductive instinct under severe stress, Induces formation. At this time, the harsh stressing conditions are already apparent in the field of cultivating the mushroom mushroom, so detailed description thereof will be omitted.

When the formation of primitives is induced as described above, mushroom induction of cultured trees is induced. As a method, mushrooms are grown for 20 days under conditions of temperature of 23 to 28 ° C, humidity of 90%, carbon dioxide of 1,000 to 1600 ppm and light of 1,000 to 1500 Lux. Induces the occurrence of mushrooms.

When mushrooms are generated, the mushrooms are grown for 2 to 3 months under the conditions of 23-25 ℃, 90% humidity, 700-1,200ppm carbon dioxide, and 1,000-1,500 Lux light.

And as described above, as shown in Figures 2f to 2h, when the growth of the flower mushroom (S6) is made is collected (S7) and packaged as it is, or dried and packaged to complete the cultivation.

Unlike the conventional sawdust, the flower mushrooms cultivated by the above method sterilize and soften the logs of softwoods themselves to increase the logarithmic growth rate of the seeds, simplifying the complicated and cumbersome cultivation process and reducing the investment cost of artificial facilities. As it is minimized, new profits of rural farmers can be easily cultivated for cultivating flowering mushrooms, which not only contributes to raising farm household income, but also makes it possible to reliably cultivate eco-friendly, pesticide-free high-quality flowering mushrooms closest to natural products. By creating high added value, it is possible to sell and export domestically and overseas to contribute to foreign currency acquisition.

In the above, the present invention has been described as a method of cultivating the mushroom mushroom as an embodiment, but it is not necessarily limited thereto, and various modifications can be made without departing from the scope and spirit of the present invention.

1 is a cultivation process of the blossom mushroom according to the present invention.

2a to 2h are photographs showing the cultivation process of the blossom mushroom according to the present invention.

Claims (9)

In the cultivation method of the flower mushroom, Peeling and cutting the logs of the conifers to produce single wood; Preparing a culture tree by attaching a culture promoter to the single tree; Sterilizing the cultured tree; Inoculating the liquid spawn on the sterilized culture tree; Culturing the mycelium on the inoculated culture tree; It comprises the step of growing the mycelia of the cultured culture tree, The culture promoter is 5-15% by weight of coniferous sawdust, 5-15% by weight of wheat bran, 5-15% by weight of jade, 5-15% by weight of malt, and 40-80% by weight of water. Cultivation method of blossom mushrooms. delete The method of claim 1, After coating the heat-resistant plastic pack in the open state on the cut single-wood, the culture promoter is attached and the upper part is sealed with a plastic filter, characterized in that the cultivation method of the pine mushroom using coniferous wood. The method of claim 1, The conifer is a cultivation method of a pine mushroom using coniferous wood, characterized in that any one of larch, pine, fir, pine, beech, buckwheat, cedar. The method according to any one of claims 1, 3 and 4, Sterilizing the culture tree, First sterilizing the cultured tree at 100-105 ° C. for 30-40 hours, secondly sterilizing the first sterilized culture tree at 70-80 ° C. for 16-24 hours, and performing the second sterilization. Method of cultivating the mushroom mushrooms using coniferous wood, characterized in that it comprises the step of cooling the cultured tree to 13 ~ 15 ℃. The method according to any one of claims 1, 3 and 4, Cultivating mycelia on the inoculated culture tree, A method of cultivating a blossoming mushroom using coniferous wood, which is incubated for 2 to 3 months at a temperature of 15 to 23 ° C. and a humidity of 70 to 80%. The method according to any one of claims 1, 3 and 4, Growing the mycelia of the cultured tree, Put the culture tree cultured mycelium in a plastic pack filled with water and leave it for 15-20 days, After the 15 to 20 days left to induce the formation of carbon by irradiating with carbon dioxide and light, Inducing mushroom generation of the cultured tree induced by the primordial formation, The method of cultivating blossom mushroom using coniferous wood, characterized in that it comprises the step of growing the mushroom mushroom of the cultured tree generated by the mushroom generation. The method of claim 7, wherein The step of inducing primitive, cultivation of the flower mushrooms using coniferous wood, characterized in that subjected to severe stress by irradiating more than 15 ℃ temperature, humidity 80%, carbon dioxide 600 ~ 1,000ppm and light 1,000 ~ 2,500 Lux Way. The method according to any one of claims 1, 3 and 4, After the growth step, and harvesting the mushroom mushrooms, Method of cultivating the zinnia mushroom using coniferous wood, characterized in that further comprising the step of drying the cultivated zinnia mushroom.

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