KR100951741B1 - Biosensor - Google Patents

Abstract

Provided is an excellent biosensor capable of accurately and easily supplying a sample liquid. A biosensor having a capillary 7 capable of collecting a sample liquid and measuring a specific substance in the sample liquid, wherein the sample supply port 13 and the auxiliary sample supply port 13, which are at least two supply ports in addition to the air hole 9, are provided. The sample supply port 14 was provided, and the sample supply can be performed by the supply ports 13 and 14 either. Even when one sample supply port 13 is blocked by a fingertip or the like and the supply of the sample liquid is stopped, the sample can be quickly supplied from the other auxiliary sample supply port 14.

Description

Biosensor {BIOSENSOR}

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biosensor for analyzing a specific component in a sample liquid, and more particularly to a biosensor for collecting a small amount of sample liquid into a small test piece by capillary action and analyzing the sample.

A biosensor is a sensor which quantifies the substrate content in a sample liquid by applying the biological material as a molecular identification element using the molecular recognition ability of biological materials such as microorganisms, enzymes, antibodies, DNA, RNA and the like. That is, the substrate contained in the sample liquid is quantified by using a reaction which occurs when the biological material recognizes the target substrate, for example, consumption of oxygen by the respiration of microorganisms, enzyme reaction, luminescence, and the like. And among various biosensors, the practical use of an enzyme sensor is progressing. For example, the enzyme sensor which is a biosensor for glucose, lactic acid, cholesterol, and an amino acid is used for the medical measurement and the food industry. This enzyme sensor reduces the electron transporter by, for example, electrons generated by the reaction of a substrate contained in a sample sample liquid with an enzyme or the like, and the measuring device electrochemically measures the reduction amount of the electron transporter. By quantitative measurement, the sample is subjected to quantitative analysis.

Various forms of the structure of such a biosensor have been proposed. For example, a blood glucose value can be easily measured. A second insulating substrate is bonded to a pair of electrodes and a first insulating substrate having a reagent layer therebetween with a spacer therebetween, and between the two insulating substrates. There is a biosensor that constitutes a capillary capable of collecting a sample liquid. The capillary is configured such that blood obtained by stabbing a human body with a needle is introduced by a capillary phenomenon from a sample supply opening that opens in the cross sections of both substrates.

In such a biosensor, depending on the angle of the biosensor at the time of adhering blood to the sample supply port, blood may not be easily introduced into the capillary, and the blood adheres to the outer surface of the insulating substrate by mistake. There was this. In such a case, even if blood is supplied again, the blood adhered to the outer surface interferes and the blood is not supplied into the capillary well, resulting in a measurement error or measurement error.

In order to solve this problem, the inventors of the present invention made the end portions of both substrates constituting the sample supply port different from each other in plan view so as not to depend on the angle of the biosensor at the time of adhering blood. A biosensor has been proposed that allows blood to be well introduced into the capillary (see Patent Document 1).

The exploded perspective view and sectional drawing of the biosensor in this patent document 1 are shown in FIG. In FIG. 8, 1 is a 1st insulating board | substrate, and the measuring electrode 2, the counter electrode 3, and the detection electrode 4 which consist of an electrically conductive material are formed on this 1st insulating substrate 1. In FIG. .

The conventional biosensor 800 is formed by joining the first insulating substrate 1, the spacer 6, and the second insulating substrate 8, and a cutout portion is present in the spacer 6. The capillary 7 is formed by this. The sample 7 is introduced into the capillary 7 from the front end side of the capillary 7 by a sample supply port 13 formed by bonding and an air hole 9 provided in the insulating substrate 1. do.

Moreover, the measurement electrode 2, the counter electrode 3, and the detection electrode 4 formed on the 1st insulating board | substrate 1 are exposed in the said capillary 7, and the position overlaps with these electrodes. The reagent layer 5 is formed.

The biosensor is inserted into a measuring instrument (not shown) having terminals connected to the leads 10, 11, 12 of the electrode before the blood is introduced, and after the blood is introduced, the measuring electrode 2 and the counter electrode ( Between 3), glucose concentration is measured by detecting changes in electrical properties as the reaction between blood and reagents occurs.

Patent Document 1: Japanese Patent Application Laid-Open No. 2002-168821

Problems to be Solved by the Invention

By the way, in the recent blood glucose measurement, in order to alleviate the pain of a diabetic patient at all, it is desired to make the amount of blood collected into a smaller amount. For this reason, the development of the biosensor which made the size of the capillary which collects blood and the size of a sample supply port smaller is progressing.

However, when the miniaturization of the conventional biosensor proceeds, there is a problem that the sample supply port is easily blocked when the deformable object such as the fingertip is pressed closely.

Fig. 9 shows a state in which blood is sucked in the conventional biosensor.

Here, as shown in Fig. 9A, when the sample supply port 13 is blocked by the fingertip, the blood supply is cut off, and the blood stops on the way without being completely filled with the capillary 7. If this happens, the sample volume will be insufficient, making it impossible to measure or displaying an incorrect result. Further, even after the sample supply port 13 is blocked with a finger, the reagent layer by the blood first introduced, even if the finger is lightly removed and completely filled with blood in the capillary, as shown in Fig. 9B. There was a problem that measurement could not be performed accurately because a difference in melting occurred and a measurement deviation occurred.

Here, it is also considered that the difference between the shape of the first insulating substrate and the second insulating substrate is further increased so that the fingertip does not block the sample supply port, but this is not practical. This is because if the difference in shape is made too large, not only the inside of the capillary but also the blood squeezed out of the capillary increases, and more blood is required.

The present invention has been made to solve the above problems, and an object of the present invention is to provide a biosensor having a structure capable of reliably collecting a capillary sample even with a small amount of sample liquid.

Means to solve the problem

In order to solve the above-mentioned conventional problems, the biosensor according to the present invention is formed by joining a first insulating substrate and a second insulating substrate, and is formed by joining these two insulating substrates. A sample supply opening that is open at an end face and to which the sample liquid is adhered, a capillary in communication with the sample supply port, and the adhered sample liquid is introduced by capillary action, and located at the other end of the capillary A biosensor having an air hole in communication with the outside of the capillary, wherein the auxiliary sample supply port which communicates with the capillary and adheres to the sample supply port is introduced into the capillary, the sample At least one or more are prepared in the vicinity of the supply port.

The auxiliary sample supply port is characterized in that the through hole penetrating the sample supply port so as to leave a part of the insulating substrate is formed in the first insulating substrate or the second insulating substrate.

Further, a spacer is formed between each of the first insulating substrate and the second insulating substrate, the spacer having cutout grooves respectively formed of the sample supply port, the auxiliary sample supply port, and the capillary, and the auxiliary sample supply. A sphere is formed in the cross section of both said board | substrates. It is characterized by the above-mentioned.

Effects of the Invention

According to the biosensor according to the present invention, in particular, a biosensor having a capillary structure and measuring with a small sample amount, having the above-described configuration, the fingertip of the subject, the shoulder part or the abdomen, etc. Even if the sample supply port is blocked by the elastic skin, the sample liquid can be reliably sucked into the capillary from the auxiliary sample supply port.

1 is an exploded perspective view and a cross-sectional view of a biosensor 100 according to Embodiment 1 of the present invention,

2 is an exploded perspective view and a cross-sectional view of the biosensor 200 according to another example of the first embodiment;

3 is an exploded perspective view and a cross-sectional view of a biosensor 300 according to still another example of the first embodiment;

4 is an exploded perspective view and a cross-sectional view of a biosensor 400 according to still another example of the first embodiment;

5 is an exploded perspective view and a cross-sectional view of a biosensor 500 according to Embodiment 2 of the present invention;

6 is an exploded perspective view and a cross-sectional view of a biosensor 600 according to Embodiment 3 of the present invention;

7 is an exploded perspective view and a cross-sectional view of a biosensor 700 showing a comparative example of the present invention;

8 is an exploded perspective view and a cross-sectional view of a conventional biosensor 800,

9 is a cross-sectional view showing a state in which blood is sucked in the conventional biosensor 800,

10 is a cross-sectional view showing a state in which blood is sucked in the biosensor 100 of the first embodiment of the present invention.

Explanation of the sign

100, 200, 300, 400, 500, 600, 700, 800: Biosensor

1st insulating substrate 2measuring electrode

3: counter electrode 4: detection electrode

5: reagent layer 6: spacer

7: capillary 8: second insulating substrate

9: air hole 10, 11, 12: lead portion

13: sample supply port 14: auxiliary sample supply port

15 notch 16 blood

17: fingertips

Hereinafter, an embodiment of a biosensor according to the present invention will be described in detail with reference to the drawings taking a blood glucose value sensor as an example.

(Example 1)

1 is an exploded perspective view and a cross-sectional view of a biosensor 100 according to Embodiment 1 of the present invention.

In the biosensor 100 of the first embodiment shown in FIG. 1, 1 is a first insulating substrate formed in a substantially semi-circular shape in the vicinity of the front end, and in a rectangular shape up to the rear end thereof. On this first insulating substrate 1, the measuring electrode 2, the counter electrode 3, and the detection electrode 4 made of an electrically conductive material are formed. 8 is a second insulating substrate formed in the same shape as the first insulating substrate 1, and 6 is disposed between the first insulating substrate 1 and the second insulating substrate 8. Spacers 7 having substantially the same shape as both insulating substrates are capillaries formed to form substantially rectangular recesses in the longitudinal direction of the spacer near the front end of the spacer.

The biosensor 100 is formed by joining the first insulating substrate 1, the spacer 6, and the second insulating substrate 8, and the cutout portion as described above in the spacer 6 is formed. The capillary 7 is formed by being present. The capillary 7 includes a sample supply port 13 formed by bonding and an air hole 9 provided at a position corresponding to the vicinity of the rear end of the capillary 7 in the first insulating substrate 1. The sample sample is introduced into the inside.

In addition, 10, 11, and 12 are in the vicinity of the rear end of the said 1st insulating board 1 of the said measurement electrode 2, the counter electrode 3, and the detection electrode 4 formed on the said 1st insulating board 1. A portion is a lead of each of the electrodes 2, 3, and 4, and 13 is a space portion in front of the capillary 7 of the spacer 6, wherein the first and second insulating substrates 1, 8) It is a sample supply port which is formed between.

Moreover, the measurement electrode 2, the counter electrode 3, and the detection electrode 4 formed on the 1st insulating board | substrate 1 are exposed in the said capillary 7, and the position which overlaps these electrodes is exposed. The reagent layer 5 is formed in this.

When the measurement is performed by the biosensor 100 according to the first embodiment, a measuring device (not shown) having terminals connected to the leads 10, 11 and 12 of the electrodes 2, 3 and 4 are used. On the contrary, in the state where the biosensor 100 is inserted, the change in the electrical characteristics between the measurement electrode 2 and the counter electrode 3 is detected, and from this, the characteristics of the sample sample are analyzed.

In addition, although the detection electrode 4 functions here as an electrode for detecting the lack of a sample amount, it can also be used as a reference electrode or a part of counter electrode.

In addition, although FIG. 1 shows that each said electrode 2, 3, 4 is arrange | positioned on the 1st insulating board | substrate 1, these electrodes are not only on the 1st insulating board | substrate 1, but opposing 2nd insulating board | substrate. It may be divided and arranged on (8).

Here, as a preferable material of the 1st insulating board | substrate 1, the spacer 6, and the 2nd insulating board | substrate 8, a polyethylene terephthalate, a polycarbonate, a polyimide, etc. are mentioned. As for the thickness of a board | substrate, the thing of 0.1-5.0 mm can be used for both a 1st insulating substrate and a 2nd insulating substrate.

As the electrically conductive materials constituting the electrodes 2, 3, and 4, precious materials such as gold, platinum, and palladium, simple materials such as carbon, or composite materials such as carbon paste or precious metal paste Included. In the former case, the electroconductive layer can be easily formed on the first insulating substrate 1 or the second insulating substrate 8 by sputtering or the like, and in the latter case by screen printing.

In addition, in forming each electrode, after forming an electroconductive layer on the front surface or one part of the 1st insulating substrate 1 or the 2nd insulating substrate 8 by the sputtering method, the screen printing method, etc. mentioned above, By providing a slit using the light or the like, the electrode can be formed separately. Moreover, it is possible to form electrodes similarly also in the printing plate in which the electrode pattern was formed previously, the screen printing method using a mask plate, the sputtering method, etc.

On the electrodes 2, 3 and 4 thus formed, a reagent layer 5 containing an enzyme, an electron carrier, a hydrophilic polymer and the like is formed. Here, as the enzyme, glucose oxidase, lactate oxidase, cholesterol oxidase, cholesterol esterase, uricase, ascorbic acid oxidase, bilirubin oxidase, glucose dehydrogenase, lactate dehydrogenase and the like can be used as electron carriers. In addition to potassium ferricyanide, p-benzoquinone and its derivatives, phenazinemethalphate, methylene blue, pelocene, derivatives thereof and the like can be used.

Examples of the hydrophilic polymer include carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose, ethyl hydroxyethyl cellulose, carboxymethylethyl cellulose, polyvinyl alcohol, polyvinylpyrrolidone, and polylysine. Polyamino acid, polystyrene sulfonic acid, gelatin and derivatives thereof, acrylic acid and salts thereof, agarose gel and derivatives thereof, and the like.

Next, the capillary 7 to which blood is supplied is formed by joining the 1st insulating substrate 1 and the 2nd insulating substrate 8 through the spacer 6 between them. The sample supply port 13 to which the blood of the capillary 7 is supplied is opened to the cross section of the 1st insulating board 1 and the 2nd insulating board 8.

In the first embodiment, the thickness of the spacer 6 may be 0.025 to 0.5 mm, the width of the capillary 7 may be 0.1 to 10 mm, and the volume of the capillary 7 may be 0.1 to 5 μL. Can be.

Here, the characteristic structure in this Embodiment 1 is providing the auxiliary sample supply port 14 which penetrates the 2nd insulating substrate 8 on the capillary 7. As shown in FIG. The auxiliary sample supply port 14 is formed on the second insulating substrate 8, and then the second insulating substrate 8 is bonded to the above-described first insulating substrate 1 and the spacer 6 to form a biosensor. To complete.

By providing this auxiliary sample supply port 14, even when the sample supply port 13 is blocked at the fingertip at the time of adhesion of blood, and supply of blood from the sample supply port 13 is interrupted | blocked, it is shown in FIG. As described above, blood can be introduced into the capillary 7 from the auxiliary sample supply port 14 provided on the second insulating substrate 8, so that the capillary 7 can be completely filled with blood. have.

This auxiliary sample supply port 14 is preferably provided at a position where the sample liquid is always attached at the time of supply of the sample liquid. Hereinafter, the position, size, shape, number, etc. of providing the auxiliary sample supply port 14 will be described.

The distance between the sample supply port 13 and the auxiliary sample supply port 14, that is, the size of A shown in the sectional view of FIG. 1 is preferably at least 0.05 to 5.0 mm. When the said distance is 0.05 mm or less, two supply ports are connected and it is unpreferable since the effect as an auxiliary sample supply port may be reduced. Furthermore, in recent biosensors, where it is desired to further reduce the amount of blood, when it is 5.0 mm or more, it is difficult to simultaneously attach the sample to the sample supply port 13 and the auxiliary sample supply port 14 at the time of sample supply. It is not preferable because it becomes.

It is preferable that the area of this auxiliary sample supply port 14 is 0.1-3.0 mm <2>. In the case of an area of 0.01 mm 2 or less, the auxiliary sample supply port lacks the ability to suck the sample liquid, and there is a possibility that the supply speed may be slowed or stopped in the middle, which is not preferable. In the case of 3Omm 2 or more, the capillary must be set large, leading to an increase in the sample amount, which is not realistic.

In addition, it is preferable to process the auxiliary sample supply port 14 with a laser. In order to process a supply port, press cut, die cut, Thompson cut, etc. are also considered, but the method of the laser processing which can be microprocessed is especially preferable.

Although this auxiliary sample supply port 14 is provided on the 2nd insulating substrate 8, even if it provides in multiple numbers, a favorable effect can be acquired. In addition, as long as the shape can satisfy the above conditions, such as a circle, an ellipse, a line, a rectangle, and a triangle, a form is not restrict | limited.

The auxiliary sample supply port 14 is provided on the second insulating substrate 8, but may be provided on the first insulating substrate 1. In addition, the position, shape, and size of the auxiliary sample supply port 14 at this time are based on the above-mentioned description.

In addition, not only the shape of the biosensor 100 of Example 1 shown in FIG. 1 but the shape of the other example of Example 1 like FIG. 2, FIG. 3, and the other example of Example 1, the same effect. Can be obtained.

That is, the biosensor 200 of another example of Embodiment 1 of FIG. 2 has a plurality of auxiliary sample supply ports 14a and 14b.

In addition, another biosensor 300 of Embodiment 1 of FIG. 3 has an auxiliary sample supply port 14 having a rectangular shape.

4 shows a biosensor 400 according to another example of Embodiment 1 of the present invention, which biosensor 400 includes a first insulating substrate 1 forming a capillary 7 and The second insulating substrate 8 is bonded to each other so that the end portions viewed from the plane are positioned at different positions.

That is, in FIG. 4, the 2nd insulating board 8 and the spacer 6 protrude 0.1-1.0 mm with respect to the 1st insulating board 1 in an entrance direction.

Also in the biosensors 200, 300, and 400 of FIGS. 2, 3, and 4, the same effects as in the biosensor 100 of the first embodiment of FIG. 1 may be obtained.

In addition, when the electrode 2, 3, 4 and the reagent layer 5 for electrochemical analysis of the specific substance in a sample liquid are provided in the capillary 7, it is shown in FIG. As described above, it is preferable that these electrodes 2, 3, 4 and the reagent layer 5 are not provided at a position corresponding directly below the auxiliary sample supply port 14 of the first insulating substrate 1.

When this auxiliary sample supply port 14 is on each of the electrodes 2, 3, and 4, the sample liquid on the electrode tends to be scattered, which leads to a difference in response values, which is undesirable.

Also in the biosensors 200, 300, and 400 of FIGS. 2, 3, and 4, the same effects as those of the biosensor 100 of the first embodiment of FIG. 1 can be obtained.

In all the biosensors 100, 200, 300, and 400 described above, it is preferable that the entire surface or part of the inner wall of the capillary 7 is subjected to the surface active treatment. By performing the surfactant treatment, the sample liquid can be sucked quickly even when the area of the sample supply port is small.

In addition, it is preferable to perform surfactant treatment on the inside of the auxiliary sample supply port 14, the entire capillary inner wall, or the periphery of the auxiliary sample supply port in the capillary inner wall.

The surface of the auxiliary sample supply port 14 or the inner wall of the capillary is subjected to the surface treatment to immediately suck the sample liquid immediately after the sample liquid reaches the auxiliary sample supply port 14. Before the supply port is blocked, the sample liquid is likely to fill the capillary.

Here, the surface treatment is effective by applying a nonionic, cationic, anionic, or cationic surfactant, corona discharge treatment, or physically providing fine unevenness to the surface. Can be obtained.

As described above, according to the biosensor according to the first embodiment, even when the sample supply port 13 is clogged while the sample liquid is being supplied, the sample liquid is quickly supplied from the auxiliary sample supply port 14, so that it is accurate and easy. The sample liquid can be aspirated into the capillary 7.

(Example 2)

5 is an exploded perspective view and a cross-sectional view of the biosensor 500 according to Embodiment 2 of the present invention.

In the biosensor 500 of the second embodiment of the present invention shown in FIG. 5, an auxiliary sample supply port 14 is provided on both the first insulating substrate 1 and the second insulating substrate 8.

Thus, by providing the auxiliary sample supply ports 14 in each of the two insulating substrates 1 and 8, even when the sample is stuck from the inclined angle, it is possible to reliably suck the sample into the spacer 6. .

Further, even if a plurality of auxiliary sample supply ports 14 are provided in the same manner as in the first embodiment, good effects can be obtained.

In addition, the shape can also be a circle, an ellipse, a line, a rectangle, a triangle, etc., and is not restrict | limited.

(Example 3)

6 is an exploded perspective view and a cross-sectional view of the biosensor 600 according to the third embodiment of the present invention.

In the biosensor 600 of Example 3 shown in FIG. 6, the capillary 7 branches in a Y-shape near the distal end, one of which becomes a sample supply port 13, and the other of which is an auxiliary sample. It is a supply port 14.

In the third embodiment, by providing two sample supply ports in the spacer 6, the same effects as in the first and second embodiments can be obtained, and the sample supply port 13 and the auxiliary sample supply port 14 are provided. ) Can be processed into the spacer 6 at a time, so that it is possible to reduce the number of times of processing at the time of manufacturing the sensor.

More specific embodiments of the present invention will be described in detail.

The biosensor which consists of the following structures was used as an example.

By forming a palladium thin film having a thickness of about 8 nm on the entire surface of the insulating substrate by sputtering on the first insulating substrate made of polyethylene terephthalate, and then measuring a portion of the thin film by YAG laser to provide a slit. The electrode was divided into electrodes, counter electrodes, and detection electrodes.

On top of that, an aqueous solution containing glucose dehydrogenase as an enzyme, potassium ferricyanide as an electron carrier, and the like is dropped in a circular shape so as to cover a part of the counter electrode and the detection electrode, centering on the measurement electrode. The reagent layer was formed by drying.

In addition, a spacer made of polyethylene terephthalate and a second insulating substrate made of polyethylene terephthalate were similarly bonded therefrom.

The second insulating substrate is subjected to a surface active treatment on the surface of the sample supply port in advance, forms an air hole, and is provided with an auxiliary sample supply port at a position where the distance to the sample supply port is 0.2 mm.

By joining this, the biosensor of the same structure as shown in FIG. 1 was formed which has a capillary which becomes a capillary in which blood is guide | induced.

Moreover, in order to confirm the effect of this invention, the conventional biosensor 800 etc. (1) shown by FIG. 8, etc. (2), and the auxiliary sample supply as the biosensor 100 of this Example 1 shown by FIG. The opening areas of the sphere 14 are O.OO5 mm 2, O.O 1 O mm 2, O.O 3 O mm 2 and 0.10O mm 2, respectively ((2), (3), (4), (5)), and FIG. 2. As another biosensor 200 according to the first embodiment shown in Fig. 2, the number of auxiliary sample supply ports 14 is two (area 0.003 mm 2), two (area 0.050 mm 2), and four (area). Is 0.O1 mm 2), 9 pieces (area of 0.1 mm 2) ((6), (7), (8), (9)), and the bio of another example of Example 1 shown in FIG. As the sensor 300, the shape of the auxiliary sample suction port 14 is rectangular ((10)), and the biosensor 500 of the second embodiment shown in Fig. 5 is the first insulating substrate 1 and the first. 2 provided with auxiliary sample supply ports 14 on both sides of the insulating substrate 8 (11), provided with auxiliary sample supply ports on the first insulating substrate (12), shown in FIG. As the biosensor 600 of Example 3, the capillary 7 is Y-shaped ((13)), and as the biosensor 700 as the comparative sensor shown in FIG. 7, the second insulating substrate 8 (14) is formed in the form of a groove-shaped slit 15 at the tip of the head, and the sample supply port 13 and the auxiliary sample supply port formed by the slit 15 are connected. Made the sensor.

Then, 2 μL of blood was sufficiently injected to completely fill the sample supply port of the biosensor of the present embodiment on the fingertip, and the finger was pressed in close contact with the sample supply port to check the aspiration state of the blood when the sample supply port was blocked. .

Table 1 shows the test results.

As is apparent from Table 1, in the conventional biosensor having no auxiliary sample supply port, suction was stopped in all results when the finger was pressed against the sample supply port. This is because the sample supply port is blocked by pressurizing and contacting a soft object such as a fingertip, so that the sample liquid cannot be supplied.

In addition, when the area of the auxiliary sample port was O.OO5 mm 2, suction was delayed when the finger was pressed against the sample supply port. This is presumed to be insufficient for introducing blood into the capillary because the area of the auxiliary sample supply port is small.

In the case where the area of the auxiliary sample supply port was O.O1 mm 2 or more, even if a finger was pressed in close contact with the supply port, it was sucked quickly. This is presumably because the sample liquid was rapidly supplied from the auxiliary sample supply port even when the sample supply port was blocked and the supply of the sample liquid became the rate.

In addition, even when a plurality of auxiliary sample supply ports were provided, the same effect could be obtained as long as the sum of the area of the supply ports was at least 0.1 mm 2.

In addition, as shown in FIG. 7, when the main sample supply port and the auxiliary sample supply port are connected to each other to form a groove-shaped slit at the tip of the second insulating substrate, when the finger is pressed against the main sample supply port, the groove becomes O. Even in an area of O 1 mm 2, suction was stopped or delayed. This is because, when the sample supply port and the auxiliary sample supply port are connected, when the finger is pressed against the sample supply port, the fingertips are brought into close contact with the inside of the auxiliary sample supply port. Guess as.

In addition, the shape of the auxiliary sample suction port is rectangular (shown in FIG. 3), the auxiliary sample supply port is provided on both the insulating substrate 1 and the insulating substrate 2 (shown in FIG. 5), and the insulating substrate ( Good results were obtained with respect to the provision of the auxiliary sample supply port on 1) and the Y-shaped capillary (shown in FIG. 6).

In the case of measuring with a trace sample as in the present embodiment, if the sample supply port and the auxiliary sample supply port were separated from each other by 5 mm or more, it was difficult to bring the sample into contact at the same time, and no effect was obtained.

Also, when the area of the auxiliary sample supply port is 3 mm 2 or more, for the same reason, it is difficult to bring the specimen into contact with the entire auxiliary sample supply port, and the auxiliary sample supply port did not play a role.

The biosensor according to the present invention is a biosensor such as a cholesterol sensor, a lactic acid sensor, an alcohol sensor, an amino acid sensor, a fructose sensor, a squarose sensor, etc., in addition to a blood glucose sensor that collects and analyzes a small amount of a sample liquid in a capillary. Useful for sensors. As the sample to be used for analysis, in addition to blood, liquid samples such as drinking water and sewage can be used in addition to urine, sweat and saliva.

Claims (12)

A sample supply port which is formed by adhering a first insulating substrate and a second insulating substrate to each other, and is formed by joining the two insulating substrates, the sample supply opening being opened at one end surface of both substrates and to which the sample liquid is adhered; A biosensor having a capillary in communication with a sphere and the adhered sample liquid introduced by capillary action, and an air hole in communication with the outside of the capillary located at the other end of the capillary, At least one auxiliary sample supply port communicating with the capillary and into which the adhered sample liquid is introduced into the capillary is provided in the vicinity of the sample supply port, The auxiliary sample supply port is formed of a through hole penetrating the first insulating substrate or the second insulating substrate to leave a part of the first insulating substrate or the second insulating substrate between the sample supply port. Biosensor characterized in that. delete The method of claim 1, The auxiliary sample supply port is formed on both of the first insulating substrate and the second insulating substrate. The method of claim 1, Between the first insulating substrate and the second insulating substrate, a spacer having cutout grooves each formed of the sample supply port, the auxiliary sample supply port, and the capillary, respectively, is disposed, The auxiliary sample supply port is formed on the end faces of the two substrates instead of the first insulating substrate or the second insulating substrate. Biosensor characterized in that. The method of claim 1, A biosensor, characterized in that the sum of the opening areas of the at least one auxiliary sample supply port is from 0.1 mm 2 to 3 mm 2. The method of claim 1, And a distance between the sample supply port and the auxiliary sample supply port is 0.05 to 5 mm. The method of claim 1, Biosensor, characterized in that the through hole for the auxiliary sample supply port is manufactured using a laser. The method of claim 1, A biosensor is provided with at least a part of the surface of the first insulating substrate or the second insulating substrate facing the capillary. The method of claim 1, A biosensor comprising an electrode and a reagent layer for electrochemically analyzing a specific substance in a sample liquid on a surface of the first insulating substrate or the second insulating substrate facing the capillary. The method of claim 9, And the first insulating substrate and the second insulating substrate have different shapes at positions of ends of the biosensors in which the specimen supply ports are formed. The method of claim 10, A biosensor, wherein no electrode or reagent layer is formed on the first or second insulating substrate facing the auxiliary sample supply port. The method of claim 1, A biosensor, characterized in that the surface treatment of the auxiliary sample supply port is subjected to the surface active treatment.

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