KR100948517B1 - Cosmetic Soap Containing Chlorella Extract - Google Patents

Abstract

The present invention relates to a cosmetic soap containing chlorella extract, and more particularly, (1) pre-treatment step of washing liquid chlorella with fresh water, followed by washing with 40% -50% ethanol solution: and (2) (1) Cosmetic soap composition containing the chlorella extract obtained by the chlorella extraction method comprising the step of extracting the useful component by adding an organic solvent from the pre-treated chlorella of step) prepared by adding 5 to 20% of the cosmetic soap composition It relates to a cosmetic soap containing chlorella extract.

Chlorella, Soap, Organic Solvent, Extract

Description

Cosmetic soap containing chlorella extract

The present invention relates to a cosmetic soap containing chlorella extract.

Chlorella, a well-known green algae, is a potent green algae that rapidly assimilates carbon sources (carbon dioxide in the air), nitrogen, and other inorganic salts by photosynthesis, such as the physiology of general plants, to rapidly proliferate (dividing) their cells. . These cells contain a large amount of protein, fat, and other active ingredients, so they are important as organic resources. In addition, the industry is already rich in protein and chlorophyll, and contains special physiological action factors and vitamins and minerals that are beneficial to the human body. Therefore, they are sold as health foods, and are widely used as feed ingredients and food flavor improvers. . Actual ingredients of chlorella vary depending on the type of algae, cultivation method and conditions, but are composed of 50 ~ 60% protein, 10 ~ 30% fat, 10 ~ 25% carbohydrate, and 6 ~ 10% ash. It is also evaluated to be nutritionally excellent. On the other hand, the cell membrane surrounding the cell is thicker and harder than yeast, but the digestibility can be increased by drying or elution.

On the other hand, in general, the skin is discharged sebum through the pores through the pores and sebum is secreted through the pores to prevent moisture evaporation and maintain the softness of the skin. It is common to use soap to effectively remove these wastes, and existing soaps are manufactured for the purpose of simply removing wastes, but are not effectively used when various problems occur on the skin. .

In general, the trouble that occurs on the skin is caused by the microbes in the air parasitic where sweat and sebum secreted a lot, causing inflammation, itching and odor of the skin. Accordingly, conventional soaps serve to protect the skin from bacteria by removing dirt attached to the skin, and to clean and disinfect the skin, but to solve various skin problems as described above. In order to produce a new soap has come.

The present invention has been made in accordance with the requirements as described above, the present invention is a chlorella extract extracted by a method of pretreatment of chlorella to loosen the thick cell wall to facilitate the elution of chlorophyll a, chlorophyll b and beta carotene such as intracellular useful substances To provide a cosmetic soap composition containing, and to provide a cosmetic soap prepared by adding this composition to provide an excellent beauty soap moisturizing, relieve acne, soothing atopic skin.

In order to solve the above problems, the present invention (1) pre-treatment step of washing the liquid chlorella with fresh water, then washed with 40% -50% ethanol solution: and (2) organic from the pre-treated chlorella of step (1) It provides a cosmetic soap composition containing a chlorella extract obtained by a chlorella extraction method comprising the step of adding a solvent to extract the useful ingredient.

The present invention also provides a cosmetic soap containing chlorella extract prepared by adding 5 to 20% of the cosmetic soap composition.

The present invention can provide an effective beauty soap that provides excellent moisturizing power, relieves acne, and soothes atopic skin by providing a cosmetic soap containing a cosmetic soap composition containing chlorella extract.

In order to achieve the object of the present invention, the present invention is a pretreatment step of washing (1) liquid chlorella with fresh water, and then 40% -50% ethanol solution: and (2) the pretreated chlorella of step (1) It provides a cosmetic soap composition containing a chlorella extract obtained by a chlorella extraction method comprising the step of extracting a useful ingredient by adding an organic solvent from the.

The pretreatment step of the composition according to an embodiment of the present invention comprises the steps of (a) repeating the method of removing and concentrating the supernatant by centrifugation after putting clean fresh water in a liquid chlorella in a 1: 1 weight ratio; (b) adding 40% -50% ethanol solution to the chlorella of step (a) in a 1: 1 weight ratio, followed by centrifugation to remove the supernatant and concentrating twice; And (c) 40% -50% ethanol solution in a 1: 1 weight ratio of chlorella in step (b), followed by centrifugation to remove the supernatant and recovering only chlorella paste.

Extracting the useful component by adding an organic solvent from the pretreated chlorella according to one embodiment of the present invention comprises (a) adding at least 95% ethanol in a 1:10 weight ratio to the pretreated chlorella paste for 3-4 hours. Mixing by using a centrifugation to separate the supernatant and chlorella paste; (b) adding 95% or more of ethanol in a 1:10 weight ratio to the separated chlorella paste, mixing the mixture for 3-4 hours using a stirrer, and centrifuging to separate the supernatant and chlorella paste; And (c) recovering the supernatant of steps (a) and (b). The ethanol is at least 95%, preferably 95%. Extracting the useful component by adding an organic solvent from the pretreated chlorella paste of the present invention was performed twice, but may be additionally repeated within the range to achieve the effect of the present invention.

Chlorella extract according to one embodiment of the present invention is characterized by exhibiting antibacterial activity, hemolytic activity and whitening effect. Chlorella extract of H. Emory V. Para Ti Syracuse KCTC2471 (Vibrio parahaemolyticus KCTC2471) and Edward tar is NUF251 (minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for Edward tarda NUF251) showed 125 ㎍ / mL, activity of the two strains to 250 ㎍ / mL, respectively. The hemolytic activity of chlorella extract was found to be 3% in 1.0 mg / mL chlorella extract. The tyrosinase inhibitory activity IC ₅₀ of chlorella ethanol extract was found to be 10.87 mg / mL.

The present invention prepares a cosmetic soap containing chlorella extract prepared by adding 5 to 20% by weight of the cosmetic soap composition. Preferably 20 wt% is added. To prepare a cosmetic soap containing chlorella extract according to the present invention can be prepared by the addition of essences and fragrances which are generally well known, and can be prepared by compression molding in a conventional manner.

Beauty soap according to one embodiment of the present invention is characterized by having a moisturizing effect, acne relief effect and atopy relief effect.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Experiment method

<1. Chlorella Pretreatment for Chlorella Organic Solvent Extraction>

① Freshwater Wash

In order to remove impurities from chlorella, clean fresh water is added to the concentrated liquid chlorella Co., Ltd. at a weight ratio of 1: 1, and then centrifuged to remove the supernatant.

② Wash ethanol solution

50% ethanol solution was mixed in a 1: 1 weight ratio to the chlorella concentrate washed three times with fresh water, followed by centrifugation to remove the supernatant.

③ Second ethanol solution washing

50% ethanol solution was mixed in a 1: 1 weight ratio to the chlorella paste washed five times by the method of ① and ②, followed by centrifugation to remove the supernatant and recover only chlorella paste.

<2. Chlorella Organic Solvent Extraction>

① Extraction of Primary Ethanol Solution

95% ethanol was added in a 1:10 weight ratio to the chlorella paste recovered in ③ of Experimental Method 1, and mixed with a stirrer for about 3-4 hours, followed by centrifugation to recover the supernatant.

② Extraction of Second Ethanol Solution

95% ethanol is added in a 1:10 weight ratio to the chlorella paste recovered in the first ethanol extraction process of ① and mixed using a stirrer for about 3-4 hours, followed by centrifugation to recover the supernatant.

<3. Analysis of Useful Components of Extracted Chlorella>

Chlorophyll was analyzed using HPLC (Acme 9000 & Nanospace SI-2, Korea). As a positive control, purity 90% chlorophyll isolated from Gwangju Institute of Science was used. Extraction conditions using HPLC (Acme 9000 & Nanospace SI-2, Korea) are as follows. Capcell-Pak C18 (4.6 × 250 mm: Shiseido Co. Ltd, Japan) was used for the column, the injection volume was 20 uL, the flow rate was 1.0 mL / min, and the wavelength was 450 nm, a solvent (Mobile Phase) was performed at room temperature using MeOH: DCM: CH ₃ CN: H ₂ O (67.5: 22.5: 9.5: 0.5).

<4. Activity Analysis of Useful Components of Extracted Chlorella>

① antimicrobial activity

As Gram-positive bacteria to investigate chlorella's minimal inhibitory concentration (MIC) and minimal bacteriocidal concentration (MBC) Bacillus Subtilis PM125 ( Bacillus subtilis PM125), Bacillus Rickenio Formis ( Bacillus) licheniformis), microcode Caicos Lou Proteus KCT0C1056 (Microccus luteus KCT0C1056) and Staphylococcus Aureus KCTC1916 ( Staphylococcus aureus KCTC1916), etc., and Escherichia as gram-negative bacteria . Collie D31 ( Escherichia coli D31), Esseria Collie KCTC1184 ( Escherichia coli KCTC1184), Enterobacter erotic Jean's KCTC2190 (Enterobacter aerogenes KCTC2190), Klebsiella Pneumoniae KCTC 2208 (Klebsiella pneumoniae KCTC 2208) , luge Pseudomonas labor KCTC2004 (Pseudomonas aeruginosa KCTC2004) and S. typhimurium KCTC 1925 was used (Salmonella typhimurium KCTC 1925), Fish Pathology bacteria is Vibrio para H. Emory Tea Syracuse KCTC2471 ( Vibrio parahaemolyticus KCTC2471) and Edward Tarda NUF251 ( Edward tarda NUF251) was used.

② antifungal activity

The antifungal activity was measured by the liquid growth assay method. Candida albicans KCTC1940 ( Candida albicans KCTC1940) was incubated at 30 ° C. for 48 hours using PDB (potato dextrose broth, Difco) medium. The concentration of the fungus was diluted to 1 × 10 ⁶ CFU / mL, and the 96 well plate (96 well plate) was mixed with the fungal culture solution and serially diluted chlorella extract. They were incubated at 30 ° C. for 48 hours and then measured at 630 nm using a microplate multi-detection reader (Synergy HT, Bioteck, USA).

③ Measurement of free radical scavenging power (antioxidant effect)

DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to measure free radical scavenging activity. After dissolving 16 mg of DPPH in 100 mL of ethanol, 500 μL of DPPH stock solution was added to 50 μL of the sample, followed by reaction for 30 minutes at room temperature by adding ethanol to a total volume of 1.0 mL. Photometer (Mecacys, Co. Ltd, Korea) is measured at 517 nm. Ascorbic acid was used as a standard sample. The antioxidant effect was measured using the absorbance of the sample as (As) and the absorbance of the standard sample as (Ao).

Radical Scavenging Activity  ( RSA ,%) = [( Ao - As ) / Ao ] X 100

Ao : absorbance of standard sample

As : absorbance of the sample

④ Tyrosinase inhibitory activity showing whitening effect

Inhibition of tyrosinase enzyme activity using Tyrosine as a substrate was performed by modifying the method of Ishihara et al. ( J. Invest , Dermatol ( 1993) 100 , 126-131). To a mixture of 220 μL of 0.1 M phosphate buffer (pH 6.5), 40 μL of 1.5 mM tyrosine, and 20 μL of sample solution, 20 μL of mushroom tyrosinase (1500 U / mL to 2000 U / mL) was added at 37 ° C. After reacting for 10 minutes, 200 μL of the reaction solution was added to a 96 well plate, and the supernatant was measured at 490 nm using a microplate multi-detection reader (Synergy HT, Biotek, USA). Arbutin was used as a standard sample.

Tyrosinase inhibition activity  = 100 [1- (S-B) / C]

S: Changes in absorbance when tyrosinase and sample solution were added

B: Changes in absorbance when 0.1M phosphate buffer (pH 6.5) was added instead of tyrosinase

C: Absorbance change value when adding a solvent in which a sample is dissolved instead of a sample solution

<5. Chlorella Soap Making>

Finely chop the soap base, dissolve it using heat and cool it until it hardens again. Then, extract chlorella extract (main ingredient: chlorophyll a, b. Beta-carotene, etc.) using the organic solvent in the above manner. Put it and mix it well, then prepare soap using a soap mold.

Example 1 Chlorophyll Peak Area According to Extraction Frequency

The extracted chlorophyll was compared to the chlorophyll standard, and as a result, it could be seen that the same time period was cleanly separated into a single peak of 7 minutes (retention time) (FIG. 1). When quantitatively compared with chlorophyll standard, it was found that 30% of chlorophyll was contained.

<Example 2. Activity Evaluation of Useful Components of Extracted Chlorella>

① antibacterial and antifungal activity

Chlorella elliposidea C020) The antimicrobial and antifungal activity of ethanol extracts was determined by the minimal inhibitory concentration (MIC) and the minimal bacteriocidal concentration (MBC). In the results of Table 1 and Figure 3, Bacillus , Gram-positive bacteria Subtilis PM125 (Bacillus subtilis PM125) and Bacillus Rickenio Formis ( Bacillus licheniformis ) showed activity at 125 ㎍ / mL with minimum inhibitory concentration and minimum bactericidal concentration.

In particular, Bacillus sp., A Gram-positive bacterium, showed strong activity. In Gram-negative bacteria (Gram-negative bacteria) of fish pathogenic bacteria Vibrio para H. Emory Tea Syracuse KCTC2471 (Vibrio parahaemolyticus KCTC2471) and Edward Tarda NUF251 ( Edward Only tarda NUF251) showed activity at 125 μg / mL and 250 μg / mL, respectively. On the other hand, it did not show activity against the fungus C. albicans .

Table 1. Minimum Inhibitory Concentration and Minimum Sterilization Concentration of Chlorella Extract

       ② hemolytic activity

Chlorella on the erythrocyte membrane (Chlorella elliposidea C 020) ethanol Human erythrocytes were used to investigate the hemolytic activity of the extract. Chlorella for human red blood cells (Chlorella elliposidea C020) showed a very low activity of 3% even at 1.0 mg / mL, the highest concentration of ethanol extract. These results demonstrate that chlorella ethanol extract is a good drug candidate that does not damage red blood cells (FIG. 4).

 ③ Tyrosinase inhibitory activity showing whitening effect

Chlorella Chlorella elliposidea C020) Inhibition of tyrosinase enzyme activity of ethanol extract was measured using tyrosine as a substrate. The tyrosinase inhibitory activity IC ₅₀ of chlorella ethanol extract was found to be 10.87 mg / mL (FIG. 5). It is IC ₅₀ of green tea extract It showed better activity than 2000 mg / mL and IC _{50 of the} extract of Park Tae-ki leaves. The value showed activity higher than 20-50 mg / mL. Specifically, chlorella hydrolyzed extract IC ₅₀ from previous studies It can be seen that the value shows significantly higher activity than 120 mg / mL. These results proved that Chlorella ethanol extract, chlorophyll, can be used as an excellent whitening functional material and is a candidate material to create high added value as a raw material of cosmetics. (FIG. 5).

<Example 3. Efficacy test of beauty soap containing chlorella extract>

Soap prepared in Experimental Method 5 was prepared in a 10% aqueous solution, sufficiently absorbed in the gauze portion of the band-aid, and then applied to the biceps muscles of the soft arm of the skin to check the skin condition after 48 hours. The test subjects were 10 general public and the score was averaged by dividing into 5 levels. The evaluation table is shown in Table 2. Results averaged 0.13, indicating little skin irritation.

Table 2. Skin irritation evaluation table of cosmetic soap containing chlorella extract

score condition 0   No irritation on the skin One   Slight irritation to skin 2   Partial erythema appears 3   Overall erythema and skin swelling 4   Skin erythema and skin splitting and bursting

In addition, using the general alkaline soap sold in the market and the soap prepared in Experimental Method 5, 10 females in their 20s, 10 male and female high school students with acne, 10 children 5-7 years old with atopy After 30 days of washing in the morning and evening, the results are shown in Tables 3, 4 and 5.

Table 3. Moisturizing Skin of Women in their 20s

Improved No change Worse Chlorella cosmetic soap of the present invention 8 people 2 people 0 Control Alkaline Soap 2 people 7 people 1 person

After using the chlorella beauty soap of the present invention, more than 80% of the moisturizing power was improved to maintain a moist skin, and accordingly it was investigated that the makeup is well received.

Table 4. High School Students of Acne Skin

Improved No change Worse Chlorella cosmetic soap of the present invention 7 people 2 people 1 person Control Alkaline Soap 2 people 4 people 6 people

After using the chlorella beauty soap of the present invention, more than 70% of the acne skin was improved.

Table 5. Atopy Soothing Effects

Improved No change Worse Chlorella cosmetic soap of the present invention 9 One 0 Control Alkaline Soap 0 2 8

More than 90% of the chlorella cosmetic soaps of the present invention were found to have a positive response by experiencing a soothing effect of atopic skin.

1 is a diagram showing a process of extracting useful components from chlorella using ethanol.

Figure 2 is a diagram showing the chlorophyll content of the extract containing useful components from chlorella.

Figure 3 shows the antimicrobial activity of chlorella ethanol extract. (A) Bacillus Rickenio Formis ( Bacillus licheniformis ), (B) Bacillus Subtilis PM125 (Bacillus subtilis PM125). (Lane 1; 100 mg / mL, 2; 50 mg / mL, 3; 25 mg / mL, 4; 12.5 mg / mL)

Figure 4 shows the hemolytic activity of chlorella ethanol extract.

Figure 5 shows tyrosinase inhibitory activity showing a whitening effect of chlorella ethanol extract.

Claims (6)

(1) repeating the method of adding clean fresh water in a liquid chlorella in a 1: 1 weight ratio and centrifuging to remove the supernatant and concentrating it three times; (2) adding 40-50% ethanol solution in a 1: 1 weight ratio to the chlorella of step (1), followed by centrifugation to remove the supernatant and concentrating twice; (3) mixing 40-50% ethanol solution in a 1: 1 weight ratio to chlorella in step (2), and then centrifuging to remove the supernatant and recovering only chlorella paste; (4) adding 95% or more of ethanol in a 1:10 weight ratio to the pretreated chlorella paste of step (3) and mixing using a stirrer for 3-4 hours, followed by centrifugation to separate the supernatant and chlorella paste; (5) adding 95% or more of ethanol in a 1:10 weight ratio to the separated chlorella paste, mixing the mixture for 3-4 hours using a stirrer, and centrifuging to separate the supernatant and chlorella paste; And (6) a cosmetic soap composition containing a chlorella extract obtained by a chlorella extraction method comprising recovering the supernatant of steps (4) and (5). delete delete The cosmetic soap composition of claim 1, wherein the chlorella extract has an antibacterial activity and a whitening effect. Cosmetic soap containing a chlorella extract prepared by adding 5 to 20% of the cosmetic soap composition of claim 1 or claim 4.          The cosmetic soap of claim 5, wherein the cosmetic soap has a moisturizing effect, acne relieving effect, and atopy relieving effect.

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