KR100886401B1 - 세포질내 정자 주입에 의한 포유류의 유전자 도입 - Google Patents
세포질내 정자 주입에 의한 포유류의 유전자 도입 Download PDFInfo
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- KR100886401B1 KR100886401B1 KR1020017001838A KR20017001838A KR100886401B1 KR 100886401 B1 KR100886401 B1 KR 100886401B1 KR 1020017001838 A KR1020017001838 A KR 1020017001838A KR 20017001838 A KR20017001838 A KR 20017001838A KR 100886401 B1 KR100886401 B1 KR 100886401B1
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- sperm
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Abstract
Description
Claims (21)
- 외래성 핵산을 비인간(non-human) 포유동물 유래의 막이 파괴된 정자머리 또는 막이 제거된 정자머리와 일정기간 배양하는 단계;상기 외래성 핵산과 상기 정자머리를 비인간 포유동물 유래의 미수정 난모세포로 공동삽입하여, 유전자도입 수정 난모세포를 형성하는 단계;상기 유전자도입 수정 난모세포를 유전자도입 엠브리오로 발생시키는 단계;를 포함하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제1항에 있어서, 상기 공동삽입 단계가 피에조 전기 작동 미세주입에 의해 수행되는 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제2항에 있어서, 상기 외래성 핵산과 상기 정자머리가 상기 미수정 난모세포의 세포질 내로 공동주입되는 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제1항에 있어서, 상기 정자머리가 동결 및 해동된 정자로부터 얻어지는 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제1항에 있어서, 상기 정자머리가 재수화된 동결건조 정자로부터 얻어지는 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제1항에 있어서, 상기 정자머리가 핵 및 핵주위 물질을 포함하는 막이 제거된 머리인 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제6항에 있어서, 상기 정자머리가 세정제로 처리된 정자로부터 얻어지는 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제1항에 있어서, 상기 미수정 난모세포가 분열중기 Ⅱ의 난모세포인 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 삭제
- 제1항에 있어서, 상기 기간이 30초 내지 5분인 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제10항에 있어서, 상기 기간이 45초 내지 3분인 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제11항에 있어서, 상기 기간이 1분 내지 2분인 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제1항에 있어서, 상기 외래성 핵산이 하나의 도입유전자 이상을 포함하는 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제1항에 있어서, 상기 유전자도입 엠브리오를 살아있는 자손으로 발생시키는 단계를 더 포함하는 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 제14항에 있어서, 상기 발생시키는 단계는 상기 유전자도입 엠브리오를 대리모로 이식하는 하위 단계를 포함하는 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 삭제
- 제1항에 있어서, 상기 비인간 포유동물이 비인간 영장류, 양, 소, 돼지, 곰, 고양이, 개, 말 및 설치류로 이루어진 군에서 선택되는 것을 특징으로 하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
- 삭제
- 삭제
- 삭제
- 비인간 포유동물 유래의 막이 파괴된 정자머리 또는 막이 제거된 정자머리를 얻는 단계;상기 정자머리를 원하는 유전자를 포함하는 외래성 핵산과 혼합하는 단계;상기 정자머리와 외래성 핵산의 혼합물을 비인간 포유동물 유래의 분리된 미수정 분열중기 Ⅱ 난모세포로 공동주입하여, 유전자도입 수정 난모세포를 형성하는 단계; 및상기 유전자도입 수정 난모세포를 유전자도입 엠브리오로 발생시키는 단계;를 포함하는 비인간 포유동물 유전자도입 엠브리오를 얻는 방법.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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US9607898P | 1998-08-11 | 1998-08-11 | |
US60/096,078 | 1998-08-11 | ||
US13397099P | 1999-05-13 | 1999-05-13 | |
US13425199P | 1999-05-13 | 1999-05-13 | |
US06/133,970 | 1999-05-13 |
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KR20010072437A KR20010072437A (ko) | 2001-07-31 |
KR100886401B1 true KR100886401B1 (ko) | 2009-03-02 |
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US (1) | US20060130163A1 (ko) |
KR (1) | KR100886401B1 (ko) |
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KR101169762B1 (ko) * | 2010-02-25 | 2012-07-31 | 건국대학교 산학협력단 | 신규한 난모세포 매개된 유전자 이식 방법 및 그를 통한 형질전환 배아 생산방법 |
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1999
- 1999-08-10 KR KR1020017001838A patent/KR100886401B1/ko not_active IP Right Cessation
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2005
- 2005-11-02 US US11/265,523 patent/US20060130163A1/en not_active Abandoned
Non-Patent Citations (4)
Title |
---|
Biol Reprod. Vol.55:789-795 (1996.10.) * |
Biol Reprod. Vol.55:789-795 (1996.10.)* |
Cell, Vol.57:717-723 (1989.06.02.) * |
Cell, Vol.57:717-723 (1989.06.02.)* |
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