KR100884086B1 - Extracting method of feed additives using a coptic rhizome and forsythia fruit extract - Google Patents

Abstract

In the present invention, 500 g (50% by weight) of Rhizome extract in 1 kg of the present invention; 100 g (10% by weight) of alum extract; dextrose 300 g (30% by weight); (10% by weight) of a licorice extract, and a method of extracting the same.

As described above, the present invention provides a method for preventing and treating diseases caused by immune enhancement and foreign substances through the promotion of growth through the improvement of feed efficiency and feed efficiency of livestock and the proliferation of immune cells (lymphocytes) This has the advantage of being used as a substitute for a feed additive containing antibiotics because it provides a feed additive to which an anticancer characteristic against a pathogenic bacterium is utilized so that a disease prevention effect is exhibited.

Hwangryun extract, Yeonjong extract, Feed additives, Extract

Description

TECHNICAL FIELD [0001] The present invention relates to a method for extracting a feed additive using a fermented soybean curd refuse, a fermented soybean curd refuse,

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is an explanatory view for explaining a composition ratio of a feed additive according to an embodiment of the present invention. FIG.

FIG. 2 is a flowchart for explaining an extraction method for extracting the yellowtail and alfalfa extract which is the main component of the present invention.

The present invention relates to a method for extracting a feed additive by using an extract of Huanghin and Yongkyo, and more particularly, to a method for extracting an antibiotic for preventing disease by feeding an aliquot The present invention relates to a method for extracting a feed additive using an alum extract.

Feed additives commonly used in the livestock industry are, for example, so-called efficiency enhancers which are added in small amounts to the feed to improve feed utilization and daily weight gain. In addition to these efficiency enhancers, medicines are usually added to feed. However, among the conventional feed additives, there are antibiotics, growth promoters, or scattering promoters. However, it is well known that the toxins of the additives can be partially absorbed into the human body through the livestock products. It is also known that the toxicity of various pesticides sprayed when cultivated in a feed such as rice bran or corn flour is also known. In particular, various antibiotics have been used for a long time for the purpose of promoting the growth of animals and preventing diseases.

However, the conventional feed additive having antibiotics as described above has recently been found to be resistant to antibiotics used in humans, as well as antibiotics used in animals, due to an increase in resistance to antibiotics used in animals and humans and the emergence of super bacteria It has become very difficult to add antibiotics to the feed of livestock products. Moreover, since such feed additives are not reliable in terms of safety, they can be substituted for antibiotics, Is required.

SUMMARY OF THE INVENTION Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a method of controlling the growth of an animal by providing a certain amount of an extract obtained from alopecia And to provide a method for extracting a feed additive using an antibiotic agent for preventing disease, which is capable of substituting for antibiotics.

Another object of the present invention is to provide a method for preventing and / or treating disease by using immune enhancement and antimicrobial properties against pathogens of foreign substances through growth promotion and immune cell (lymphocyte) proliferation by improvement in daily body weight gain and feed efficiency of livestock, The present invention also provides a method for extracting feed additives using a fermentation extract and a fermented yeast extract, which is used as a substitute for a feed additive containing antibiotics.

In order to attain the above object, the present invention provides a method for producing a medicament for preventing or treating cancer, comprising administering 500 g (50% by weight) 100 g (10% by weight) of alum extract; dextrose 300 g (30% by weight); 100 g of licorice extract (containing 10% by weight of phytol.

Another feature of the present invention is to provide a method of washing a material,

A hot water treatment step of collecting the extracts after heating the hot water for a predetermined period of time after putting the bristles washed and washed in the distilled water for a certain period of time;

A repeated hot water treatment step of adding a certain amount of distilled water to the extracted solution collected after the hot water treatment step, collecting the extracted solution, adding a certain amount of distilled water to the extracted solution, heating again, and collecting the extracted solution;

And a lyophilization step of collecting and concentrating the extracted liquid several times after the repeated hot water treatment step and then lyophilizing the concentrated extract.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

As shown in Fig. 1, the present invention provides 500 g (50% by weight) of Rhizome extract in 1 kg of the present invention; 100 g (10% by weight) of alum extract; dextrose 300 g (30% by weight); 100 g (10% by weight) of licorice extract were mixed to prepare a feed additive.

In another embodiment of the present invention, 500 g (50% by weight) of Rhizome extract in 1 kg of the present invention; dextrose 300 g (40% by weight); 100 g (10% by weight) of licorice extract were mixed to prepare a feed additive.

In another embodiment of the present invention, 600 g (60% by weight) of tangle extract in 1 kg of the present invention; 350 g dextrose (35 wt%); And 50 g (5% by weight) of licorice extract to prepare a feed additive.

It is preferable to add 100 to 400 g of the feed additive of the present invention prepared in the above composition ratio per ton of feed.

Herein, Forsythia koreana is mainly composed of lignans such as arctigenin, arctin and phillyrin, caffeic acid glycosides such as forsythiaside and suspensoside, and triterpenoids such as betulinic acid and ursolic acid. Detoxification, drainage, inflammation and diuretic effects.

In addition, it has been found that the chrysanthemum is mainly composed of berberine and its alkaloid components such as palmatine, jateorrhizine and coptisine, and has dryness, sedative and anti-inflammatory and antimicrobial effects in herbal medicine.

Since the present invention is inferior to chlortetracycline (CTC), which is widely used as a conventional feed additive, by using a small amount of extract which can be easily obtained from licorice, chrysanthemum, dextrose and licorice, which are used as natural medicines, It was invented as an antibiotic substitute.

Next, the operation and effect of the present invention as described above will be described.

As shown in FIG. 2, the method of extracting the pectus and the phloem of the feed additive according to the present invention is as follows. First, the process proceeds from the initial state S1 to the material washing step S2, Wash with appropriate washing intensity to avoid spillage.

Then, after the material washing step (S2), the process proceeds to the hot water treatment step (S3), and the washed brass and the bridges are put in distilled water and heated for a predetermined time after being called for a certain time.

In addition, after the hot water treatment step (S3), the process proceeds to the step of repeating the hot water treatment (S4). After a certain amount of distilled water has been added to the extracted solution, a certain amount of distilled water is added to the extract solution, Collect the extract.

After the repeated hot water treatment step (S4), the process proceeds to the freeze-drying step (S5), and the extracted liquid which is extracted several times is collected and concentrated, and the concentrated extract is freeze-dried.

That is, in the method of extracting the main components of the feed additive of the present invention, firstly, 100 g of the alum and alfalfa are washed in water to remove dust and then washed. Since most of the materials are water-soluble, Wash as lightly as possible because the minute is leaked.

Then, the thus-washed phlynx and alveoli are placed in a white bag used for storing medicinal materials, and then put in 1000 ml of distilled water for about 20 to 30 minutes, soaking the medicine for 150 minutes, and collecting the extract.

In addition, 800 ml of distilled water is added to the extract solution collected by the primary hot water treatment as described above, and the extract solution is collected for 150 minutes. 700 ml of distilled water is added to the extract solution, and the extracted solution is collected for 150 minutes.

Then, the extracts obtained by three times as described above are collected and concentrated to 100 ml. At this time, be careful not to burn the concentrate, discard the charcoal extract and re-extract it. However, 100 ml of concentrated extract that has been normally extracted is freeze-dried.

Herein, the contents of the alum and alfalfa extract extracted by the above extraction method are as follows.

 That is, 1) the concentration of the sample in the alumina sample; 100 ml, dried amount; 1.82 g / 20 ml, extraction efficiency; 9.1 g / 100 g (%). 2) Concentration of thrips sample; 100 ml, dried amount; 2.67 g / 20 ml, extraction efficiency; 13.3 g / 100 g (%).

Examples of the antibacterial activity tests of the alum extract and alfalfa extract extracted by the extraction method of the present invention

   First, the antimicrobial activity of the alum and its extracts of the present invention and the two mixed extracts of the present invention against major pathogens of livestock were confirmed.

In addition, the extracts of Staphylococcus aureus were stronger than the extracts of Staphylococcus aureus and showed strong antimicrobial activity against causative bacteria of respiratory diseases such as Pasteurella and Staphylococcus aureus such as Escherichia coli and Salmonella strains (Table 1 and Table 2).

These test results indicate that these drug extracts can be usefully used for disease prevention purposes.

[Table 1] Developmental inhibition effect of major pathogenic microorganisms of allium and chrysanthemum extract

[Table 2] Minimum growth inhibitory concentration (MIC) measurement of pathogenic bacillus thuringiensis extracts

The safety test for mice using a combination of a saccharide and a chrysanthemum extract, which are the main components of the feed additive of the present invention

   As a result of the safety test on the mouse of the alopecia brassicae extract extracted by the extracting method of the present invention, it was confirmed that the alum extract was safe even when 0.2 ml of the crude solution (25%) was inoculated. On the other hand, the extracts of Chrysanthemum morifolium were found to be toxic, such as the death of 0.2 ml of the diluted solution (25%) to 20 times dilution (1.25%). This shows that the use of a good amount of medicines is toxic and the use of a small amount of toxic substances is a good medicine.

[Table 3] Safety in mice of alfalfa and alfalfa extract

The disease control effect on the experimental animal (mouse) of the extract of the present invention,

  In the disease control effect test of the extract of Huangliao and Quercus mongolica extracts, the mice were orally inoculated with 7 kinds of pathogens such as E. coli for 7 days and then administered for 3 days. The survival rate of the mice was 48% In the case of alfalfa extract, it was 52%, which was about three times as much as that of the untreated control group.

Effect of Growth and Immune Enhancement in Pigs as a Feed Additive Containing Yeast and Chrysanthemum Extract, which is the Main Component of the Present Invention <Example 1>

  (ADG) and feed efficiency (FCR) were 3.5% and 8.3%, respectively, and 9.6%, respectively, in the extracts of Chrysanthemum morifolium extract and Chrysanthemum morifolium extract, respectively. And 4.7%, respectively. The lymphocyte proliferation was also increased by 8.1% and 6.5%, respectively, compared to the non - supplemented group, indicating the possibility of using antibiotic substitute as a feed additive.

A) Configuration of Test Sphere

B) Glycemic effect on piglets

C) Effect on pig body weight gain (ADG)

 D) Effect on feed intake (ADFI) of piglets

E) Influence on proliferative activity of piglets

Effect of Growth and Immune Enhancement in Pigs as a Feed Additive Containing Yeast and Chrysanthemum Extract, which is a Principal Component of the Present Invention <Example 2>

In order to determine the optimum dose as a feed additive for the extracts of Pyrrhizae and Fusarium oxysporum, 50 ppm was used as a standard, and 25 ppm and 100 ppm were administered to piglets (39 days old) for the first week and 100 ppm and 300 ppm respectively The increase in body weight was greater at 100 ppm. Also, there was no synergistic effect of mixed administration of the two substances. On the other hand, when these substances were administered to piglets mixed with Salmonella and Escherichia coli, the extracts of Chrysanthemum morifolium and Chrysanthemum morifolium were more effective than those of CTC, which is an antibiotic used as a feed additive for the purpose of preventing disease or promoting growth, After 20 days of drug administration, Salmonella and Escherichia coli were negative in liver, lung, and intestine in the group treated with extract of Huanglung extract. In the group of Salmonella and Escherichia coli, Of the patients were able to confirm the treatment effect of the disease compared with the positive of salmonella and 2 of 3 colonies.

A) Configuration of Test Sphere

 I) In piglets Weight effect

* No statistically significant difference in the same row by the same letter (P = 0.05).

After 2 weeks, the mice were orally inoculated ( S. typhimurium 1.1 × 10 11 cfu / ml, E. coli (ST + LT) 4.6 × 10 9 cfu / ml, 20 ml / both).

C) Number of bacteria collected from the feces

* Bacterial isolate positive / test head

D) Recovery of bacteria in the parenchyma

* After 3 weeks of inoculation with bacteria, isolation of bacteria in each organ

Effect of Growth and Immune Enhancement in Broiler Chicks as a Feed Additive Containing Yeast and Chrysanthemum Extract, which is the Main Component of the Present Invention <Example 3>

  The feed efficiency of 12.0% and 7.4%, respectively, were improved by 3.0% and 4.4%, respectively, compared to the control without nutrient supplementation for 42 days. In addition, a significant increase was observed in the spleen index, bursa of fabricius index, and IgA and IgM content in blood.

A) Configuration of Test Sphere

B) Gain effect on broiler chickens

C) Influence on broth growth of broiler chickens

 D) Influence on broiler immunity

  hemp) In the blood  Effects on immune function

As described above, the present invention tends to strengthen regulations on the use of antibiotics due to an increase in the resistance rate of antibiotics and an increase in the incidence of multidrug-resistant bacteria. In order to develop a substitute for antibiotics used for growth promotion and disease prevention, the antimicrobial activity of oriental medicinal herbs was investigated.

As a result of applying the extracts of these two substances to pigs and chickens, the effects of ADG and feed efficiency (FCR) improvement, immune cell lymphocyte differentiation increase effect, disease prevention and recovery promotion And as a feed additive, it could be used as a substitute for antibiotics for growth promotion and immunity enhancement. In addition, a significant increase in the spleen index, Bursa of fabricius index, and IgA and IgM content in the blood of chickens was observed. There is also an effect.

Claims (5)

delete delete delete delete A material washing step of washing 100 g of alum and alum; After the material washing step, the washed brass and brassiere are placed in 1000 ml of distilled water, heated for 20 to 30 minutes, heated again for 150 minutes, and then the extracted material is collected; A hot water treatment step in which 800 ml of distilled water is added to the extracted solution collected after the hot water treatment step, and then the extracted solution is collected, then 700 ml of distilled water is added to the extracted solution, and the heated solution is heated again to collect the extracted solution; And a lyophilization step of collecting and concentrating the extracted liquid several times after the repeated hot water treatment step and then lyophilizing the concentrated extract.

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