KR100804104B1 - Composition for beverage and health food comprising extract of peach blossom and main minerals of deep sea water - Google Patents

Abstract

The present invention relates to a composition for beverages and health foods containing the peach blossom extract and deep sea water, the composition for beverages and health food of the present invention is a component of the extract and rich mineral components present in the deep sea water to the human body When ingested, it has various beneficial effects on the human body.

Botanical Flower, Extract, Deep Sea Water, Moisturizing, Composition

Description

Composition for beverage and health food comprising extract of peach blossom and main minerals of deep sea water}

The present invention relates to a composition containing peach blossom extract and marine deep water, and more particularly, to a composition for beverages and health foods containing peach blossom extract and marine deep water extracted using marine deep water as an extraction solvent.

Peach tree is a shrub belonging to the Rosaceae family. In the medical books, "Hyang-Yak-Sang-Bang-bang" and "Agreement Bogam", "Peach blossoms cast out weak ghosts and soften their skin."

Wild peach petals have been harvested from late May to early June and stored well, and have been used as a folk remedy because the skin softens and cleansed when used in combination.

On the other hand, deep sea water means seawater collected at a depth of 200m or less without sunlight, and is not only clean but rich in eutrophication, ripening, and minerals.

Cleanliness not only means that it is not contaminated with artificial substances, but also means very little bacterial growth because of the small amount of organic matter that needs to be broken down.

Aging means "very fine" and "soft" than surface water. Due to low temperatures and high pressures, the density is high, indicating that fine particulate minerals are dissolved in sea water over time.

The abundance of minerals means that certain of the minerals in marine surface waters are reduced by living organisms, but deep waters are always constant. Some of the effects of using deep ocean water in beverages, food, cosmetics, and functional beverages are related to this mineral property.

Skin is a barrier that protects living organisms from the external environment, and is divided into three layers of epidermis, dermis, and subcutaneous tissue in order from the outside. In particular, the epidermis plays an important role in preventing the evaporation of moisture inside the human body, it develops into a thin stratum corneum while containing moisture through the differentiation process in the epidermal layer. The stratum corneum is the skin's outermost layer that is in direct contact with the external environment, preventing the invasion of mechanically and chemically harmful ingredients, preventing internal moisture from being lost in a drier external environment, and while the stratum corneum itself retains adequate moisture for flexibility. Is keeping.

Skin moisture level is about 70% in the dermis but decreases as it goes to the epidermis and becomes about 10-30% in the outermost stratum corneum. Proper moisture is maintained in the stratum corneum to maintain the elasticity and suppleness of the skin. Normally, normal skin contains about 30% water or water-soluble components.

In addition, keratinocytes of healthy people have a high concentration of Natural Moisturing Factor (NMF) to help skin moisture retention.

Meanwhile, with the advent of eating cosmetics in recent years, the general perception that "cosmetic is applied to the skin" is changing. The most important focus of these eating cosmetics is anti-aging and whitening of the skin, which is difficult to absorb vitamins and minerals through the skin, but when taken in the form of gunpowder, they reach the skin cells and improve the condition of the skin. Because you can.

In recent years, various factors such as artificial temperature control of cooling / heating due to changes in the environment and life patterns, various stresses and environmental pollution in social life, frequent face washing due to makeup habits, and natural skin aging due to age increase As a result of the lack of minerals and essential amino acid components that maintain the body's homeostasis, and drying the skin, such as the rough surface of the skin, the need for maintaining the homeostasis of the body, protect and improve the skin, have.

Accordingly, it is an object of the present invention to provide compositions for beverages and health foods that help protect the skin and maintain the homeostasis of the body.

In order to achieve the above object, the present invention provides a composition for beverages and health food containing a peach blossom extract and deep sea water.

The peach flower extract is preferably used to extract the deep sea water as the extraction solvent.

The peach flower extract is preferably contained 0.1 to 20 parts by weight based on 100 parts by weight of deep sea water.

In addition, the peach blossom is preferably a wild peach blossom.

Further, the deep sea water may use raw water, concentrated or filtered water by reverse osmosis, concentrated or filtered water by electrodialysis, or a mixture thereof.

The composition for beverages and health foods of the present invention inhibits melanin production in cells, produces intracellular collagen, inhibits intracellular fat production, inhibits intracellular histamine, antioxidant effects, skin moisturizing effect and blood alcohol concentration. Inhibitory effect.

The composition for health food of the present invention may be prepared or added in the form of a beverage, gel, powder or pills to drink or eat, and the beverage composition may be prepared in a drinkable form such as liquid or gel form.

In addition, the composition for beverages and health foods of the present invention may contain an absorption promoting substance for increasing the effects of moisturizing, wrinkle improvement, whitening, antioxidant, lipolysis and hangover.

Hereinafter, the present invention will be described in detail by way of examples. However, the examples are only to illustrate the invention and the present invention is not limited by the following examples.

Production Example  1. Deep sea water extraction and purification

The deep seawater collected at 500m depth in the East Sea of Korea was desalted by reverse osmosis system. The water produced is free of bacteria and minerals. Meanwhile, the extracted deep seawater was extracted with a high concentration of minerals using an electrodialysis apparatus.

The amount of elements contained in each of the reverse osmosis filtered water and the electrodialyzed water is shown in Table 1 below.

TABLE 1

Element (ppm) enemy Reverse Osmosis Filtrate Reverse Osmosis Concentrated Water Electric dialysis Electrodialysis concentrate Al 0.28 0.004 0.56 0.008 0.57 As ND ND ND 0.002 0.002 Ba ND ND ND ND ND Ca 419.0 0.006 687.9 9.7 713.7 CD ND ND ND ND ND Co 0.52 ND 1.6 ND 2.0 Cr 1.9 0.006 0.026 1.9 3.9 Cu 0.053 0.004 2.4 0.007 1.7 Fe 0.005 0.001 1.6 ND 1.5 Mg 1285 0.04 2586 118 2258 Mn 0.043 ND 0.053 ND 0.061 Pb <0.001 <0.001 <0.001 ND ND Sr 6.61 ND ND 0.46 1.65 K 430 5.7 560 1.78 461 Zn 0.01 0.002 0.23 0.002 0.31

The water produced in the two devices were mixed to prepare a drinkable level of mineral containing a hardness of 30 ~ 1500mg / L.

Example 1. Peach blossom  Preparation method of extract

Take 10 g of wild peach blossoms, add 100 ml of deep seawater obtained in the above preparation, extract it by boiling under reflux for 2 hours, filter it with a filter paper, and extract the filtrate. After adjusting to mL, the adjustment solution was left at 4 ° C. for 6 days, and the resulting precipitates were subjected to 0.5 μm membrane filtration to prepare peach blossom extract.

Examples 2-5. Preparation of Mixed Drinks

The peach peach flower extract prepared in Example 1 and the deep sea water were mixed in the composition shown in Table 2 to prepare a mixed drink.

TABLE 2

Example 2 Example 3 Example 4 Example 5 Stone Peach Flower Extract 5 wt% 10 wt% 15 wt% 20 wt% Deep ocean water 95 wt% 90 wt% 85 wt% 80 wt%

Experimental Example 1. B-16 Melanoma  Melanin Inhibitory Effect in (Melanoma)

The experiment was performed by the method of melanogenesis inhibition assay, which compares the amount of melanin production in the cell with the blank sample.

This experiment was performed by inoculating mouse melanoma (B-16) cells in 6-well plates at 1 × 10 ⁵ per well in DMEM containing an appropriate amount of fetal bovine serum (FBS) or a medium having an equivalent growth capacity. The cells were then incubated at 37 ° C. under 5% CO ₂ until at least about 80% were attached to the bottom of the well. After incubation, the medium was removed, the sample was replaced with a medium diluted to an appropriate concentration, and then incubated at a constant time under 37% for 5% CO ₂ . The concentration range of the sample was determined by a toxicity test using a crystal violet assay. The cells from which the medium was removed were washed with PBS (phosphated buffer saline), which was treated with trypsin to recover the cells. The recovered cells were measured using a hematocytometer and centrifuged at 5,000 to 10,000 rpm for 10 minutes, and then the supernatant was removed to obtain pellets. The cell pellet was dried at 60 ° C., and 100 μl of 1M sodium hydroxide solution containing 10% DMSO or an appropriate amount of cell lysis buffer was added to obtain intracellular melanin in a 60 ° C. thermostat. Using this solution, the absorbance at 490 nm was measured with a microplate reader to determine the amount of melanin per cell number or melanin per constant protein, and was corrected by a blank test.

On the other hand, the crystal violet analysis method is as follows.

After culturing the cells by treating a certain concentration of sample, the medium is carefully removed from the wells and washed with PBS. After removal, 50 μl of crystal violet solution (0.2% crystal violet / 2 ml ethanol, 98 ml purified water added) is added to the 96 well. Incubate for 10 minutes at room temperature and wash with purified water, taking care not to drop the cells. 100 μl of 1% SDS (sodium dodecyl sulfate) is added to dissolve the dye, and the absorbance is measured at 570 nm.

The experimental results are shown in Table 3 below.

TABLE 3

sample Treatment concentration (%) Inhibitory effect (%) Deep Marine Water Extract (Samples) One 98.5 0.5 96 0.1 87 Arbutin 0.5 75

The deep sea water extract is a sample of the above experiment, and the deep sea water having a hardness of 30 mg / l, which is extracted by peach flowers with deep sea water, is diluted by each treatment concentration, and the sample used in the following Experimental Example Diluted samples were used.

Experimental Example 2. Collagen production effect in human skin fibroblast

Intracellular Collagen Synthesis Assay was used.

Human fibroblasts (primary cell line) or similar fibroblasts (CCD-986sk, HS68, Detroit 5116, etc.) were inoculated at the bottom of the culture dish and then penicillin (100 IU / ml), streptomycin (100 μg / ml), DMEM (Dulbecco's Modified Eagle's Medium) medium containing 10% FBS (fetal bovine serum) or a medium having an equal or greater growth capacity was added thereto, and the culture was carried out in an incubator containing 5% carbon dioxide at 37 ° C. Sample concentrations of this test were determined by preliminary experiments using MTT assay or crystal violet assay, and three or more concentration ranges were determined, including concentrations that showed no cytotoxicity and were effective. Serum-free DMEM medium was used to dissolve or dilute the test substance. However, when the test substance was not dissolved in DMEM medium, it was dissolved using a suitable solvent such as ethanol.

The concentration setting preliminary test (MTT assay) method is as follows.

Incubate the cells with a certain concentration of sample, remove 10% of the medium from the wells, and then remove the amount of MTT solution (0.5% 3- (4,5-dimethyl thiazol-2-yl) -2,5 diphenyl-). 2H-tetrazolium bromide) was added and incubated for 4 hours. After removing the culture solution, add 300 µl of dimethylsulfoxide solution, shake for 10 minutes, and measure the absorbance at 570 nm with an ELISA reader.

The collagen amount measurement method is as follows.

100 μl of the antibody-PoD conjugate solution is added to the wells, followed by diluting 1/5 of the culture solution and 20 μl of the standard solution, and incubating at 37 ° C. for 3 hours. Remove the culture from the wells and then wash 4 times with 400 μl of phosphate buffer (PBS). Add 100 μl of the coloring reagent, incubate for 15 minutes at room temperature, add 100 μl of 1 N sulfuric acid, and measure it with an ELISA reader at 450 nm. a) Standard solution preparation: Add water to the collagen standard and dilute to 0, 10, 20, 40, 80, 160, 320, 640ng / ml respectively. b) Reagent: Use Procollagen type I peptide EIA kit (Takara Biomedical Co.).

The experimental results are shown in Table 4 below.

TABLE 4

sample Treatment concentration (%) Creation Effect (%) Deep Sea Water Extract (Sample) One 91.5 0.5 88 0.1 76 Vitamin c 0.1 71

Experimental Example 3. 3 T3 - L1  Fat cells ( adipocyte Inhibitory Effect on Adipogenesis

3T3-L1 fibroblasts were cultured in DMEM medium containing 10% FBS (fetal bovine serum), 4.5 g / L glucose, 2 mM glutamine and antibiotics. After cell growth, incubate in DMEM medium containing insulin (5 g / mL), 75 mM dexamethasone (0.5 mM dexamethasone) and 0.5 mM isobutylmethylxanthine and add only insulin (5 g / mL). The culture was further incubated for 3 days. It was then exchanged with DMEM / FBS medium every three days until used for the experiment. Differentiated cells were used when the lipid droplets were in the form of more than 90% of the fat cells in the form of a tightly packed fat droplet. Cells cultured in each 24-well were treated with samples diluted at 1%, 0.5% and 0.1% concentrations, and after 48 hours, intracellular fat droplets were stained with 10% Oil red O and ethanol. Extracted and quantified at 540 nm.

The experimental results are shown in Table 5 below.

TABLE 5

sample Treatment concentration (%) Inhibitory effect (%) Deep Sea Water Extract (Sample) One 95.3 0.5 81 0.1 55

Experimental Example 4. RBL -2 H3  Histamine inhibitory effect in mast cell

Hexosaminidase Quantitative methods were used.

During the series of allergic processes, various chemical delivery substances are released by the degranulation step. One way to measure this degranulation is to measure the degree of inhibition of hexosaminidase stored in granules and released with histamine from mast cells during degranulation.

Cell Preparation: RBL-2H3 mast cells (5 × 10 ⁵ cells / ml) suspended in EMEM-10 (minimum essential medium eagle) were suspended in a 24-well plate at 400 μl per well. 5 μl IgE was added and left overnight in a 5% CO ₂ incubator to sensitize the cells. 160 μl after washing with 500 μl of Shiraganian buffer (Siraganian buffer: 119 mM NaCl, 5 mM KCl, 5.6 mM glucose, 0.4 mM MgCl ₂ , 25 mM PIPES, 40 mM NaOH, 1 mM CaCl ₂ , 0.1% BSA, pH 7.2) Shiraganian buffer was added and pre-incubated at 37 ° C. for 10 minutes. Samples were added and incubated at 37 ° C. for 20 minutes, then antigens were added and incubated at 37 ° C. for 10 minutes. The reaction was terminated by standing on ice for 10 minutes, and the supernatant was taken and centrifuged for 90 seconds with a microcentrifuge. Substrate pre-incubated at 37 ° C. (dissolved P-NAG in 0.1 M citrate buffer and used to make 1 mM) was added to a 96 well plate, and the supernatant of the centrifuged cell reaction was added thereto, followed by incubation at 37 ° C. for 1 hour. 0.1 M Na ₂ HCO ₃ was added to terminate the reaction, and the absorbance was measured at 405 nm. The experimental results are shown in Table 6 below.

TABLE 6

sample Treatment concentration (%) Inhibitory effect Deep Marine Water Extract (Samples) One 99.5 0.5 93 0.1 88

Experimental Example 5. Antioxidant Effect ( DPPH , NBT )

DPPH: To investigate the inhibitory effect of hydroxyl radicals, 1-diphenyl-2-picrylhydrazyl (DPPH) solution was prepared with 80 μg / ml EtOH, and each sample was concentrated in 1 ml of the solution. After dissolving in ethanol, the absorbance at 517 nm of DPPH was measured.

NBT: 1.6ml of Triton X-100, 2.9mg of EDTA, 1mg of Nitro-blue-tetrazolium (NBT), hypoxanthine in 300mM phosphate buffer (pH 7.8) to measure the inhibitory effect of superoxide anion. After adding 5.4mg to form a substrate solution, 12µl of xanthine oxidase was dissolved in 10ml of 300Mm phosphate buffer (pH 7.8), and the samples were treated by concentration, and then quantified at 540nm.

The experimental results are shown in Table 7 below.

TABLE 7

sample Treatment concentration (%) NBT inhibitory effect DPPH inhibitory effect Deep Marine Water Extract (Samples) One 96.5 99.8 0.5 92.7 97.5 0.1 90 91 Green tea extract 0.1 78 89.6

Experimental Example 6. Electrical Conductivity with Increased Moisture in Skin ( Tewameter Synergistic effect

Transepidermal Water Loss was measured according to the method described by Pinnagoda et al. (1989) using a water evaporation meter (Tewameter TM 210 (Courage + Khazaka, Koln, Germany)) which is an evaporimeter. The room temperature was 24-26 ° C. and the relative humidity was 50-60%.

The subjects were all 10 adults (7 women, 3 men), the results are shown in Table 8 below.

TABLE 8

sample Treatment concentration (%) Synergy average Deep Marine Water Extract (Samples) 5 91.3 One 76 0.1 44

Experimental Example 7. Effect of Ethanol and Acetaldehyde Enzyme Activity (Rat) and Inhibition of Blood Alcohol Concentration

Security measures for ADH: The enzyme source was dissolved in 8 ml of 0.1% bovineserum albumin solution in lyophilized S9 rat liver homogenate (MOLTOX Co., USA) and a 0.45 µm syringe filter. It was used after filtration with. The activity of ADH was measured by modifying Blandino's method (Bostian. Et al., 1978), and the rate of NADH production at an absorbance of 340 nm was used as an index. The reaction mixture was mixed with 1.4 ml of distilled water, 0.75 ml of 1.0 M Tris-HCl buffer (pH 8.8), 0.3 ml of 20 mM NAD +, 0.3 ml of ethanol, 0.1 ml of sample and 0.15 ml of enzyme source in a cuvette. After adjusting to 3 ml and preincubating at 30 ° C. for 5 minutes, the change in absorbance at 340 nm was measured for 5 minutes. At this time, the sample was not added as a control. ADH activity of the samples was determined as relative activity (%) relative to the control.

The activity of ALDH was measured by modifying Bostian's method (Bostian. Et al., 1978), and the rate of NADH production at the absorbance of 340 nm was used as an index. The composition of the reaction solution was 2.1 ml of distilled water, 0.3 ml of 1.0 M Tris-HCl fermentation liquid (pH 8.0), 20 ml NAD + 0.1 ml, 1.0 M acetaldehyde 0.1 ml, 3.0 M KCl 0.1 ml, 0.33 M 2-mercaptoethanol 0.1 ㎖, 0.1 ml of the sample and 0.1 ml of enzyme source were put in a cuvette, adjusted to 3 ml in total, and preincubated at 30 ° C. for 5 minutes, and then the change in absorbance at 340 nm was measured for 5 minutes. At this time, the sample was not added as a control. The ALDH activity of the sample was measured as the control relative activity (%).

Blood alcohol level measurement: 10 male and female subjects (8 males and 2 females) were ingested three glasses of soju, followed by drinking 100 ml of a 30 mg / l mixed beverage prepared for each concentration. Blood alcohol concentration was measured using. The blood alcohol degrading effect was calculated from the formula 1- (experimental blood alcohol concentration / control group (when not mixed drink) blood alcohol concentration) x 100.

The experimental results are shown in Table 9 below.

TABLE 9

sample Treatment concentration (%) ADH activity ALDH activity Blood Alcohol Degradation Effect Deep Sea Water Extract (Sample) 5 90.4 95.4 71.8 One 86.7 91.2 56 0.1 51.2 76.1 24

As described above, the composition for beverages and health foods according to the present invention contains marine deep water together with peach blossom extract, and the rich mineral components present in the peach blossom extract and marine deep water nourish the body through ingestion. Not only does it provide a boost, but it can also improve skin condition.

Claims (8)

A health food composition comprising peach flower extract and marine deep water extracted using deep sea water as an extraction solvent. delete delete The method of claim 1, The peach flower extract is a health food composition, characterized in that it contains 0.1 to 20 parts by weight based on 100 parts by weight of deep sea water. The method of claim 1, The peach flower is a health food composition, characterized in that the wild peach blossom. The method of claim 1, The deep sea water is selected from concentrated water by reverse osmosis, filtered water by reverse osmosis, concentrated water by electrodialysis, filtered water by electrodialysis and mixtures thereof. The method of claim 1, The health food composition is a health food composition, characterized in that it is prepared in the form of one of a liquid drink, gel or pills. The method of claim 1, The composition has the effect of inhibiting melanin production in cells, intracellular collagen production, intracellular fat production inhibitory effect, intracellular histamine inhibitory effect, antioxidant effect, skin moisturizing effect and blood alcohol concentration inhibiting effect The composition for healthy food.