KR100696954B1 - Green tea extracts and thereof manufacturing method - Google Patents

Abstract

Provided are a green tea extract, which has a strong anti-oxidative effect, is highly soluble in water, and maintains the taste of green tea, and a manufacturing method thereof. The method for manufacturing green tea extracts having an anti-oxidative action comprises the steps of: collecting a good quality of green tea leaves to be steamed over heat of 80‹C; mixing the green tea leaves, Lycii fructus, and Cornus officinalis in a weight ratio of 7:2:1, putting the mixture into a soy jar together with sugar, enzymes, and spring water, and fermenting the mixture for 3 years or more; and screening the fermented green tea, Lycii furctus, and Cornus officinalis to obtain a liquid.

Description

Green tea extract and antioxidant manufacturing method for antioxidant activity

The present invention relates to a green tea extract and a method for producing the same, and more particularly, to a green tea extract and a method for producing the same for enabling antioxidant activity by mixing and extracting green tea, goji berry, and cornus.

Usually, the human body is always at risk of oxidative stress due to free radicals generated by normal or abnormal internal conditions or environmental substances.

In other words, reactive oxygen reacts with nucleic acids (DNA), proteins, lipids, carbohydrates, etc. in vivo, making these molecules unable to perform their normal functions and causing diseases such as cardiovascular diseases, nervous system problems, immune system disorders, cancer, and promoting aging. It is known.

Therefore, inhibiting the production of free radicals in vivo not only prevents various diseases, but also emerges as a very important factor in inhibiting aging, and thus research on this has been very active recently.

Aging is a series of changes over time in the process of human development, growth, and maturation, physiological phenomena leading to morphological and mechanical decline and death, which can lead to decreased cell division, increased cell death, And it is related to the decrease in the number of cells that occur due to the dysfunction of the remaining cells.

In particular, the classification of aging mechanisms includes the misunderstanding that an error according to the gene itself or genetic information causes protein binding abnormalities, a somatic mutation that a somatic mutation causes a cell dysfunction, a hypothesis that it is inherited according to a predetermined program, or overuse Aging occurs due to DNA protein defects, immunological theory that causes autoimmune diseases due to reduced immunity, and free radicals in cells promotes aging. The theory being discussed is the theory of free radicals presented by Harman, whose molecular oxygen in vivo causes free radicals to be produced by catalysis of oxidases, damaging cells and degrading their function.

In general, the human body undergoes energy metabolism using oxygen, and in the process, free radicals, which are harmful free radicals, are produced, and oxidation of proteins, cell membranes, fats, nucleic acids, etc. in the body causes cancer, arthritis, diabetes, and atherosclerosis. Not only causes various diseases, but also promotes skin aging.

Furthermore, the human body has a balance of prooxidants and antioxidants, but when these balances are imbalanced by various factors and tilted toward the oxidation promoters, oxidative stress is induced. Phosphorus cell damage and pathological disease. Reactive oxygen species (ROS), which are a direct cause of this oxidative stress, are by-products generated by most living organisms that breathe. The process uses oxygen molecules as the final electron acceptor of the electron transport system to obtain energy. Can come from. That is, oxygen, which occupies about 20% of the atmosphere, is indispensable for aerobic metabolism, and 1-4% of oxygen absorbed into the cell is not completely reduced during electron transfer and is transferred to free radicals. Will be displayed. In addition, ROS can be produced in the process of detoxifying foreign substances, phagocytosis of phagocytes, and normal metabolism of various oxidases in cells, and continue to be produced in the body during life. In particular, oxygen radicals derived from ROS account for the largest portion of free radicals.

Modern civilization has provided the rationality and convenience of daily life, but the generation factors of free radicals due to environmental pollution and destruction of ozone layer are continuously generated in living organisms. These free radicals are highly reactive and react with proteins, lipids, sugars, nucleic acids (DNA), etc., which are the constituents of cells, causing damage to cells and tissues, causing various adult diseases and aging. Therefore, the suppression and elimination of their production is important for disease prevention and public health improvement.

Therefore, the present invention causes oxidative stress and causes aging, cancer, cardiovascular diseases and other pathological diseases. Thus, the present invention is intended to manufacture a substance for blocking ROS. Recently, medicinal plants and natural products contain free radicals harmful to the human body. It has been found that the antioxidant active substances to remove a lot, but the present invention was able to obtain a strong antioxidant extract by extracting the extract by mixing green tea, wolfberry, and cornus in the most suitable combination.

In addition, the present invention is sold by extracting the green tea in the conventional tea bags, unlike the conventional method of drinking in drinking water, so it is easily dissolved in water, so that it is possible to drink quickly, and to extract the green tea by fermentation so that you can enjoy the taste of green tea as it is It was.

The present invention will be described in detail as follows.

The present invention is to take a good quality green tea leaves steaming at 80 ℃ heat, and the mixing ratio (weight ratio) of the green tea leaves, goji berry, and cornus oil, respectively 7: 2: 1, and mixed with sugar, enzymes, and bottled water Green tea extract containing goji berry and cornus oil is obtained by putting it in a container (jangdok) and fermenting it for at least 3 years and filtering the fermented green tea, goji berry, and cornus oil through a sieve.

It is preferable to use Tommy enzyme as sugar and sulfur sugar as sugar. When the amount of green tea leaves, wolfberry and cornus oil is 70 kg of green tea leaf, 20 kg of wolfberry, 10 kg of cornus oil, the amount of sugar is 45 kg, Tommy 100g of enzyme is added.

Water was used for the bottled water, and 3 liters were added to mix well with the above ingredients. This mature green tea extract is diluted with a 1: 5 ratio of the stock solution and water.

The amount of the enzyme, sugar and bottled water is increased proportionally according to the amount of green tea leaves and wolfberry, cornus.

Green tea refers to tea made from unfermented tea leaves and has been a favorite food for a long time in human history. The composition of green tea varies slightly depending on the type of tea tree, cultivation conditions, harvest season, harvesting site, soil quality, manufacturing method, tree age and washing, but usually polyphenols, caffeine, protein, amino acids, carbohydrates, pigments and various organic acids. And enzymes, vitamins, and inorganic components. Among these ingredients, especially tannins and caffeine, vitamin C, flavonoids, and inorganic ingredients are known to benefit the human body, such as antioxidant activity, anticancer action, blood cholesterol lowering effect and cardiac action, so that the general public is interested in green tea. As a result, green tea consumption is increasing every year.

Goji is widely known as an excellent medicine that can be reinforced regularly in ancient medicines such as new agricultural herbaceous plant, Dongbobom, herbal herb, and medicinal introductory, and has pharmacological effects such as nourishing tonic and soy sauce protection. It is known to have antibacterial effect, anticancer effect, immune boosting effect, liver function improvement, blood pressure lowering and antidiabetic effect, antioxidant effect, blood cholesterol lowering effect, and it is known to be effective in treating and preventing geriatric diseases such as whitening and astringent effect. .

Cornus is one of the glycosides of tannin and saponin, such as cornin that excites parasympathetic nerves, loganin, which has some central nervous excitability, and saponin. Organic acids such as gallic acid, tartaric acid and malic acid, and tannin and vitamin A, which are astringent ingredients, are effective in nourishing and astringent effects, and selenium can prevent cancer. It has functional components, such as (Se).

In the present invention, the present inventors experimented as follows to find the most suitable combination of green tea, wolfberry, and cornus oil.

Example

First, extracts were prepared from a mixture of green tea, green tea and goji berry, and cornus oil, and their radical scavenging effect, that is, the inhibitory effect was examined.

At this time, DPPH (Sigma chemical Co. USA) and other solvents were used as first-class reagents, and L-ascorbic acid (Junsei, Japan) as a control. Absorbance was measured with a microplate reader (Emax, USA). Six 100 μL of each sample was seeded on each 96-well plate and 100 μL of each sample in the blank, and 100 μL of 60 μM DPPH was added to the test group. In the control, 100 μL of MeOH was seeded instead of the sample, and then 100 μL of DPPH was added, and 100 μL of EtOH was added to the blank instead of DPPH. After leaving at room temperature for 30 minutes, the absorbance was measured with a microplate reader at 540 nm to investigate the absorbance due to the reduction of DPPH. The absorbance difference between the sample adder and the control was expressed as a percentage, and the results are shown in Table 1.

 Experimental material  10 μg / mL concentration  5 μg / mL concentration  Remarks  green tea  74%  21%  Green Tea (80%)-Cornus (20%) Mixture  55%  8%  Green Tea (80%)-Golgija (10%)-Horse oil (10%) mixture  53%  11%  Green Tea (70%)-Cornus (30%) Mixture  43%  3%  Green Tea (70%)-Golgija (10%)-Horse oil (20%) mixture  44%  5%  Green Tea (70%)-Golgija (20%)-Horse oil (10%) mixture  82%  50%

       As described above, the present invention showed a particularly strong antioxidant activity when only one green tea was used, that is, when the mixture of green tea and wolfberry and cornus oil was used rather than a single green tea extract, and the mixing ratio of green tea and wolfberry and cornus oil (weight ratio) 7: 2: 1 was the most effective.

As such, the present invention causes oxidative stress to cause aging, cancer, cardiovascular diseases and other pathological diseases, and thus has the effect of providing a substance for blocking ROS, and the most suitable combination of green tea, wolfberry, and cornus Look for a more powerful antioxidant that can provide an extract.

In addition, the present invention is sold by extracting the green tea in the conventional tea bags, unlike the method of drinking and drinking in the water, so that it is easily dissolved in water, so that it is possible to drink quickly, extract the green tea by fermentation, so you can enjoy the taste of green tea as it is It has a very useful effect.

Claims (2)

      Take quality green tea leaves and steam them at 80 ℃ heat, and mix the green tea leaves, goji berry and wild corn oil in a ratio of 2: 2: 1, mix them with sugar, enzymes and bottled water, and add them to Jangdok 3 Green tea extract production method for antioxidant activity, characterized in that it is prepared by the step of fermentation for more than a year, and the step of filtering only the fermented green tea and gojija, cornus oil through a sieve.        Green tea, wolfberry, and cornus oil composition is a method for producing green tea extract for antioxidant activity, characterized in that each of the ratio of 7: 2: 1 is added (weight ratio).