CN112280626A - Preparation method of medical industrial hemp beer - Google Patents

Preparation method of medical industrial hemp beer Download PDF

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Publication number
CN112280626A
CN112280626A CN202011242146.9A CN202011242146A CN112280626A CN 112280626 A CN112280626 A CN 112280626A CN 202011242146 A CN202011242146 A CN 202011242146A CN 112280626 A CN112280626 A CN 112280626A
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beer
fermentation
wort
malt
tank
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麦力艳
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Hainan Feilaili Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00Other raw materials for the preparation of beer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00Fermentation processes for beer
    • C12C11/02Pitching yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00Other raw materials for the preparation of beer
    • C12C5/02Additives for beer
    • C12C5/026Beer flavouring preparations
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00Preparation of wort
    • C12C7/20Boiling the beerwort
    • C12C7/205Boiling with hops

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
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  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
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  • Microbiology (AREA)
  • Mycology (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)

Abstract

The invention discloses a preparation method of medical industrial hemp beer, relates to a preparation method of industrial hemp beer, and aims to solve the problems of single taste and single nutrient content of the existing beer. The preparation method comprises the following steps: firstly, weighting Pierson malt, caramel malt, dried medical industrial hemp flowers and leaves, hops, calcium chloride and calcium sulfate; secondly, crushing the malt; thirdly, adding water and the ground malt material into a saccharifying tank for saccharifying; inputting the mash into a filter tank for filtering treatment; fifthly, boiling; sixthly, adding the medical industrial cannabis sativa flowers and leaves into the filter tank; seventhly, carrying out precipitation and convolution treatment; eighthly, fermentationTransferring the solution into a fermentation tank, and adding beer dry yeast; ninth, sealing the fermentation tank and filling CO2And performing after-fermentation treatment. The beer of the invention is blended with the special aroma and health care effect of the medical industrial hemp, and the prepared beer has special flavor and aroma and contains various components of the medical industrial hemp which are beneficial to human bodies.

Description

Preparation method of medical industrial hemp beer
Technical Field
The invention relates to a preparation method of industrial hemp beer.
Background
The beer is brewed by using malt as main material and adding hop, gelatinizing and saccharifying the mixture and then fermenting the mixture in liquid state. The beer has low alcohol content, contains various amino acids, vitamins and cellulose, and also contains iron, calcium, phosphorus, antioxidants and the like, and the nutrient components are easily absorbed and utilized by human bodies, so the beer is often called liquid bread. The medical industrial cannabis sativa is an industrial cannabis sativa variety which is improved by a breeding technology and has the content of Tetrahydrocannabinol (THC) lower than 0.3 percent and the content of beneficial cannabinoids such as CBD (cannabidiol). Cannabis sativa L.in pharmaceutical industry contains cannabinoid, flavone, stilbenoid, terpene, lignanoid, alkaloid, etc., and has more than 400 trace compounds. There are hundreds of cannabinoids, the highest CBD content, which not only has no dependence and addiction, but also has inhibitory effect on the dependence and addiction of THC (tetrahydrocannabinol) in cannabis plants. Research shows that CBD has the functions of diminishing inflammation, relieving pain, relieving anxiety, resisting depression, resisting insomnia, protecting nerves, promoting cardiovascular health, treating epilepsy, treating mental disease and reducing withdrawal symptoms, and has important value in the aspects of medicine, health food, cosmetic development and the like. Cannabis plants in pharmaceutical industry also contain cannabinoids such as CBC (cannabichromene), CBG (cannabigerol), and CBN (cannabinol), which have proven to be of high medical value. The aroma of industrial hemp plants is determined by the type of terpenes they contain. Terpenes are one of the most common varieties of natural products. They have a variety of functions in plants and are commonly used in food products and food supplements. Industrial cannabis contains hundreds of terpenes, which not only provide taste and aroma, but also produce synergistic effects with cannabinoids (CBD, CBN, CBG, etc.), enhancing the beneficial effects of various cannabinoids.
Disclosure of Invention
The invention aims to solve the problems of single taste and single nutrient content of the existing beer, and provides a preparation method of medical industrial hemp beer with special flavor.
The preparation method of the medical industrial hemp beer is realized according to the following steps:
weighing 87-90.5% of Pierson malt (with the color of 3-4.9), 5-8% of caramel malt (with the color of 3-5), 2.5-3.2% of dried Cannabis sativa flowers and leaves in the pharmaceutical industry, 1.2-1.5% of hops, 0.05-0.25% of calcium chloride and 0.05-0.25% of calcium sulfate as dry material raw materials according to weight percentage;
secondly, crushing: crushing the Pearson malt and caramel malt in the dry material raw materials, and uniformly mixing to obtain a ground malt material;
thirdly, saccharification: adding water and ground malt into a saccharification tank, controlling the blanking temperature to be 45-55 ℃, standing, adding part of calcium chloride and part of calcium sulfate into dry material raw materials, adjusting the pH value of a system to be 5-6, carrying out gradient heating to completely saccharify starch, and heating to 75-80 ℃ to inactivate amylase to obtain mash;
fourthly, filtering: inputting the mash obtained in the step three into a filter tank for filtering treatment to obtain filtrate, adding water into the filter tank, stirring, collecting wort, and transferring the filtrate and wort into a boiling tank;
fifthly, boiling: boiling the filtered juice and the wort in a boiling tank, adding hops accounting for 5-15% of the weight of the hop raw materials and the residual calcium chloride and calcium sulfate after boiling, and adding 30-50% of the weight of the hop raw materials before boiling to obtain the boiled wort;
sixthly, secondary filtering: adding medicinal industrial hemp flowers and leaves in dry raw materials into a filter tank, transferring boiled wort into the filter tank, simultaneously adding (a small amount of) cold water to reduce the temperature of the wort to 75-80 ℃, controlling the contact time of the wort and the medicinal industrial hemp flowers and leaves to be 5-10 minutes, fully allowing terpene fragrant substances and various beneficial components in the medicinal industrial hemp to enter the wort, and filtering to remove the medicinal industrial hemp flowers and leaves to obtain filtered wort;
seventhly, deposition convolution treatment: adding the rest hop into the filtered wort, carrying out precipitation and convolution treatment, then cooling to 16-20 ℃, and oxygenating to obtain fermentation liquor;
eighthly, fermentation: transferring the fermentation liquor into a fermentation tank, inoculating 80-100 g of beer dry yeast into one hundred liters of beer, carrying out open fermentation, and carrying out constant-temperature fermentation at 18-22 ℃ for 7-9 days to finish primary fermentation;
ninth, after-fermentation: sealing the fermentation tank and filling CO2And (3) boosting the pressure to 0.12-0.13 mpa, performing after-fermentation treatment, and keeping the fermentation temperature at 3-5 ℃ to obtain the medical industrial hemp beer.
On the basis of the existing fine brewing beer production process, flowers and leaves of the hemp in the medicinal industry are added in the brewing process, and on the basis of the original color and flavor of the beer, the special aroma and the health care effect of the hemp in the medicinal industry are integrated, so that a new beer product which has good aroma and taste, contains various components beneficial to the human body of the hemp in the medicinal industry and has certain relaxation, calming effect and health care effect is developed. The existing industrial hemp beer production method generally adopts hemp seeds as raw materials, the hemp seeds are mature and dry seeds of industrial hemp, and have certain physiological health care efficacy on human bodies, but poisoning can be caused by excessive eating. The invention adopts the flower and leaf of industrial hemp as raw materials, and has different health care effects due to different components. The terpene substances and CBD beneficial cannabinoids in cannabis used in pharmaceutical industry are only produced in flowers and leaves, where terpenes can impart special aroma and flavor to beer, while hemp seeds and pure CBD do not. Moreover, CBD is only one of many cannabinoids and has limited health care functions. The purification of CBD, whether by alcohol extraction or more advanced supercritical extraction, is inevitable with some contaminants and residues. The invention is made from flowers and leaves of the cannabis sativa in the pharmaceutical industry, retains various components beneficial to human bodies and the original natural flavor of plants in the cannabis sativa in the pharmaceutical industry to the greatest extent, can be safe without residues, and even can reach the standard of organic food.
The invention adds the dried flowers and leaves of the medical industrial hemp in the brewing process, and various components and proportions in the manufacturing process, and the prepared beer contains special flavor and fragrance, contains various components of the medical industrial hemp which are beneficial to human body, has the efficacies of relieving anxiety, joyful mood, strengthening heart and brain, protecting bones and the like, can inhibit the side effect of alcohol, and reduces hangover.
The raw hemp beer prepared by the method has the concentration of 11.5-13 DEG P, the alcoholic strength of 3-5% Vol, golden color, turbidity of suspended yeast, moderate and lasting foam, and has the fragrance and taste of citrus fruits.
Detailed Description
The first embodiment is as follows: the preparation method of the medical industrial hemp beer is implemented according to the following steps:
weighing 87-90.5% of Pierson malt (with the color of 3-4.9), 5-8% of caramel malt (with the color of 3-5), 2.5-3.2% of dried Cannabis sativa flowers and leaves in the pharmaceutical industry, 1.2-1.5% of hops, 0.05-0.25% of calcium chloride and 0.05-0.25% of calcium sulfate as dry material raw materials according to weight percentage;
secondly, crushing: crushing the Pearson malt and caramel malt in the dry material raw materials, and uniformly mixing to obtain a ground malt material;
thirdly, saccharification: adding water and ground malt into a saccharification tank, controlling the blanking temperature to be 45-55 ℃, standing, adding part of calcium chloride and part of calcium sulfate into dry material raw materials, adjusting the pH value of a system to be 5-6, carrying out gradient heating to completely saccharify starch, and heating to 75-80 ℃ to inactivate amylase to obtain mash;
fourthly, filtering: inputting the mash obtained in the step three into a filter tank for filtering treatment to obtain filtrate, adding water into the filter tank, stirring, collecting wort, and transferring the filtrate and wort into a boiling tank;
fifthly, boiling: boiling the filtered juice and the wort in a boiling tank, adding hops accounting for 5-15% of the weight of the hop raw materials and the residual calcium chloride and calcium sulfate after boiling, and adding 30-50% of the weight of the hop raw materials before boiling to obtain the boiled wort;
sixthly, secondary filtering: adding the medicinal industrial cannabis sativa flowers and leaves in the dry material raw materials into a filter tank, transferring the boiled wort into the filter tank, simultaneously adding (a small amount of) cold water to reduce the temperature of the wort to 75-80 ℃, controlling the contact time of the wort and the medicinal industrial cannabis flowers and leaves to be 5-10 minutes, and filtering to remove the medicinal industrial cannabis flowers and leaves to obtain filtered wort;
seventhly, deposition convolution treatment: adding the rest hop into the filtered wort, carrying out precipitation and convolution treatment, then cooling to 16-20 ℃, and oxygenating to obtain fermentation liquor;
eighthly, fermentation: transferring the fermentation liquor into a fermentation tank, inoculating 80-100 g of beer dry yeast into one hundred liters of beer, carrying out open fermentation, and carrying out constant-temperature fermentation at 18-22 ℃ for 7-9 days to finish primary fermentation;
ninth, after-fermentation: sealing the fermentation tank and filling CO2And (3) boosting the pressure to 0.12-0.13 mpa, performing after-fermentation treatment, and keeping the fermentation temperature at 3-5 ℃ to obtain the medical industrial hemp beer.
The cannabis flowers and leaves used in the pharmaceutical industry in this embodiment are not crushed or otherwise lose their original taste and aroma. The industrial hemp flower leaf contains components such as tannin for increasing bitterness, and the contact time and temperature of the industrial hemp flower leaf with wort are controlled to adjust in the preparation process, so that the components are prevented from entering beer too much.
In the embodiment, calcium chloride is added in the boiling process, wherein the chloride ions can adjust the pH value of the mash and the wort, protect the activity of alpha-amylase and contribute to the flocculation of protein and the stability of taste, and the calcium is a mineral element necessary for the vital activity of the beer yeast, can promote the growth of the beer yeast and reduce the influence of adverse environment on yeast cells. The addition of the calcium sulfate helps to stabilize the taste of the beer, sulfate ions have acidity increasing effect, can promote the action of carboxypeptidase and chloropeptidase, reduce the pH value, promote the decomposition of enzyme on a substrate in the saccharification process, and the calcium chloride also helps to form fragrance and promote the balance and stability of the fragrance of hops and hemp flower leaves in the pharmaceutical industry in the beer.
This embodiment mixes wort with pharmaceutical industrial cannabis at elevated temperatures during the re-filtration process, where heating activates the activity of cannabinoids and terpenes, some of which are produced by heating, such as cannabidioic acid (CBDA) which is converted to CBD by decarboxylation after heating. At the same time, the temperature is preferably kept from being too high, about 80 ℃, so as to avoid the loss of the hemp fragrance and reduce the leaching of tannins and polyphenol compounds.
The embodiment adopts flowers and leaves of industrial hemp as raw materials, and has different components and different health care effects compared with the hemp seeds. The beer contains terpene substances, and can bring special aroma and flavor to beer. While hemp seed and pure CBD have no special aroma and flavor. The industrial hemp flower and leaf contains components such as tannin which can increase the bitter and astringent feeling, and the contact time and temperature of the industrial hemp flower and leaf with wort are controlled to adjust the components in the preparation process, so that the components are prevented from entering the wine too much.
The embodiment combines the medical industrial hemp with the daily-drinking beer to produce a new beer product which has good aroma and taste, and has certain relaxing, calming and health-care effects.
The second embodiment is as follows: the difference between the present embodiment and the first embodiment is that the mass ratio of the industrial fried dough twists and the leaves used in the first step is (10-30) to (70-90).
The third concrete implementation mode: the difference between the first embodiment and the second embodiment is that 88 to 90.5 weight percent of Pearson malt, 5 to 7 weight percent of caramel malt, 2.5 to 3.1 weight percent of dried Cannabis sativa flowers and leaves in pharmaceutical industry, 1.3 to 1.5 weight percent of hops, 0.05 to 0.2 weight percent of calcium chloride and 0.05 to 0.2 weight percent of calcium sulfate are weighed in the first step.
The fourth concrete implementation mode: this embodiment differs from one of the first to third embodiments in that water and ground malt material are added to the mashing tank in step three, wherein the mass of the water and ground malt material is 2: 1.
The fifth concrete implementation mode: the difference between the embodiment and one of the first to the fourth embodiments is that 50 wt% of calcium chloride and 50 wt% of calcium sulfate are weighed in the dry material raw materials added in the third step, and the pH value of the system is adjusted to be 5-6 by using lactic acid.
The sixth specific implementation mode: the difference between this embodiment and one of the first to fifth embodiments is that the gradient temperature rise in the third step is performed by first preserving the heat at 67 ℃ for 35 to 45 minutes and then preserving the heat at 72 ℃ for 10 minutes.
The seventh embodiment: the difference between the first embodiment and the sixth embodiment is that cold water is added in the sixth step, the temperature of wort is reduced to 75-80 ℃, and the contact time between wort and hemp flowers and leaves in the pharmaceutical industry is controlled to be 8-10 minutes.
The specific implementation mode is eight: the difference between this embodiment and the first to seventh embodiments is that the wort is filtered by a filter sieve plate in the filter tank in the fourth step.
The specific implementation method nine: the difference between this embodiment and the first to eighth embodiments is that in the fourth step, water is added into the filtering tank repeatedly to filter and collect wort 2-4 times.
The detailed implementation mode is ten: the difference between this embodiment and the first to ninth embodiments is that the time of the precipitation and whirling treatment in the seventh step is 20 to 40 minutes.
The concrete implementation mode eleven: the difference between this embodiment and the first to tenth embodiments is that the cell concentration after the fermentation liquid inoculation in the step eight is 600 ten thousand to 1200 ten thousand/mL.
The specific implementation mode twelve: the present embodiment is different from the first to the eleventh embodiments in that the time of the post-fermentation treatment in the ninth step is 12 to 15 days.
Example (b): the preparation method of the medical industrial hemp beer of the embodiment is implemented according to the following steps:
weighing 180 kg of Pearson malt (color 3-4.9), 10.1 kg of caramel malt (color 3-5), 6 kg of medical industrial hemp flowers and leaves (the mass ratio of the flowers to the leaves is 20: 80), 400g of bitter hops (Northern Brewer Northern hop), 2500g of scent hops (Saaz Czechz hops), 100g of calcium chloride and 100g of calcium sulfate as dry material raw materials;
secondly, crushing: crushing the Pearson malt and caramel malt in the dry material raw materials, and uniformly mixing to obtain a ground malt material;
thirdly, saccharification: adding 400L of water and ground malt into a saccharification tank, controlling the blanking temperature to be 52 ℃, standing for 10 minutes, adding 50g of calcium chloride and 50g of calcium sulfate, adjusting the pH value of a system to be 5.3 by using lactic acid, stirring, heating to 67 ℃, preserving heat for 40 minutes, preserving heat for 10 minutes at 72 ℃ until starch is completely saccharified, and heating to 78 ℃ to inactivate amylase in malt to obtain mash;
fourthly, filtering: inputting the mash obtained in the third step into a filter tank, standing for 20min for filtering treatment to obtain filtrate, adding 200L of water, stirring, collecting the wheat juice for the second time, adding 200L of water again, collecting the wheat juice for the third time, and transferring the filtrate and the wheat juice into a boiling tank;
fifthly, boiling: boiling all the wort collected in the fourth step, adding 400g of northern brewed hop, the rest 50g of calcium chloride and 50g of calcium sulfate after boiling, boiling for 1 hour, and adding 1250g of Czechz hop 20 minutes before finishing to obtain boiled wort;
sixthly, secondary filtering: adding medicinal industrial hemp flowers and leaves in dry material raw materials into a filter tank, transferring boiled wort into the filter tank, simultaneously adding a small amount of cold water, reducing the temperature of the wort to 80 ℃, controlling the contact time of the wort and the medicinal industrial hemp flowers and leaves to be 10 minutes, fully allowing terpene fragrant substances and various beneficial components in the medicinal industrial hemp to enter the wort, and filtering to remove the medicinal industrial hemp flowers and leaves to obtain filtered wort;
seventhly, deposition convolution treatment: adding the rest 1250g of Czechz hops into the filtered wort, performing precipitation and convolution treatment for 30 minutes, cooling to 18 ℃, and oxygenating to obtain fermentation liquor;
eighthly, fermentation: transferring the fermentation liquor into a fermentation tank, inoculating 85 g of beer dry yeast into one hundred liters of beer, carrying out open fermentation, and fermenting at the constant temperature of 20 ℃ for 8 days to finish primary fermentation;
ninth, after-fermentation: sealing the fermentation tank and filling CO2Increasing pressure to 0.12mpa, performing after-fermentation for 14 days, maintaining fermentation temperature at 4 deg.C to obtain medicated industrial hemp beer, bottling under constant pressure.
The raw wort concentration of the hemp beer in the medical industry prepared by the embodiment is 11.5-13 DEG P, the alcoholic strength is 3-5% Vol, the color is golden yellow, the beer has turbidity of suspended yeast slightly, the foam is moderate and lasting, and the beer has fragrance and taste of citrus fruits.
First, physicochemical detection indexes:
according to the physical and chemical detection method of beer in the national standard GB/T4928-2008, the prepared beer is subjected to sensory analysis, and the result is as follows:
item Physical and chemical indexes
Alcohol degree% v/v 4.93+/-0.06
pH value 4.21+/-0.1
Total acid 16.0/+/-1.0
Acidity of volatility 1.6+/-0.32
Sulfur dioxide mg/l 2+/-1
Raw wort content% Plato 11.7+/-0.1
Secondly, sensory analysis of beer:
according to the sensory analysis method of the beer in the national standard GB/T4928-2008, the prepared beer is subjected to sensory analysis, and the result is as follows:
Figure BDA0002768776510000061
Figure BDA0002768776510000071
thirdly, the contents of cannabidiol, tetrahydrocannabinol and cannabigerol in the industrial cannabis beer are determined by adopting a high performance liquid chromatography as follows:
cannabinoid Content (wt.)
CBD 0.014mg/L
THC 0.006mg/L
CBG 0.002mg/L

Claims (10)

1. The preparation method of the medical industrial hemp beer is characterized in that the preparation method is realized according to the following steps:
weighing 87-90.5% of Pierson malt, 5-8% of caramel malt, 2.5-3.2% of dried hempseeds and leaves in the pharmaceutical industry, 1.2-1.5% of hops, 0.05-0.25% of calcium chloride and 0.05-0.25% of calcium sulfate as dry material raw materials according to weight percentage;
secondly, crushing: crushing the Pearson malt and caramel malt in the dry material raw materials, and uniformly mixing to obtain a ground malt material;
thirdly, saccharification: adding water and ground malt into a saccharification tank, controlling the blanking temperature to be 45-55 ℃, standing, adding part of calcium chloride and part of calcium sulfate into dry material raw materials, adjusting the pH value of a system to be 5-6, carrying out gradient heating to completely saccharify starch, and heating to 75-80 ℃ to inactivate amylase to obtain mash;
fourthly, filtering: inputting the mash obtained in the step three into a filter tank for filtering treatment to obtain filtrate, adding water into the filter tank, stirring, collecting wort, and transferring the filtrate and wort into a boiling tank;
fifthly, boiling: boiling the filtered juice and the wort in a boiling tank, adding hops accounting for 5-15% of the weight of the hop raw materials and the residual calcium chloride and calcium sulfate after boiling, and adding 30-50% of the weight of the hop raw materials before boiling to obtain the boiled wort;
sixthly, secondary filtering: adding the medicinal industrial cannabis flowers and leaves in the dry material raw materials into a filter tank, transferring boiled wort into the filter tank, simultaneously adding cold water, reducing the temperature of the wort to 75-80 ℃, controlling the contact time of the wort and the medicinal industrial cannabis flowers and leaves to be 5-10 minutes, and filtering to remove the medicinal industrial cannabis flowers and leaves to obtain filtered wort;
seventhly, deposition convolution treatment: adding the rest hop into the filtered wort, carrying out precipitation and convolution treatment, then cooling to 16-20 ℃, and oxygenating to obtain fermentation liquor;
eighthly, fermentation: transferring the fermentation liquor into a fermentation tank, inoculating 80-100 g of beer dry yeast into one hundred liters of beer, carrying out open fermentation, and carrying out constant-temperature fermentation at 18-22 ℃ for 7-9 days to finish primary fermentation;
ninth, after-fermentation: sealing the fermentation tank and filling CO2And (3) boosting the pressure to 0.12-0.13 mpa, performing after-fermentation treatment, and keeping the fermentation temperature at 3-5 ℃ to obtain the medical industrial hemp beer.
2. The method for preparing Cannabis beer for pharmaceutical industry according to claim 1, wherein the mass ratio of the industrial fried dough twists and leaves used in the first step is (10-30): (70-90).
3. The method for preparing cannabis beer for pharmaceutical industry according to claim 1, wherein 88 to 90.5 percent of pearson malt, 5 to 7 percent of caramel malt, 2.5 to 3.1 percent of dried cannabis flowers and leaves for pharmaceutical industry, 1.3 to 1.5 percent of hops, 0.05 to 0.2 percent of calcium chloride and 0.05 to 0.2 percent of calcium sulfate are weighed according to the weight percentage in the first step.
4. The process for the preparation of cannabis beer according to claim 1, characterised in that in step three water and ground malt mass are added to the mashing tank, wherein the mass of water and ground malt mass is 2: 1.
5. The preparation method of the cannabis beer for pharmaceutical industry according to claim 1, characterized in that 50 wt% of calcium chloride and 50 wt% of calcium sulfate weighed out from dry materials are added in the third step, and the pH value of the lactic acid adjusting system is 5-6.
6. The method for preparing Cannabis beer for pharmaceutical industry according to claim 1, wherein the gradient temperature rise in step three is performed by first keeping the temperature at 67 ℃ for 35-45 minutes, and then keeping the temperature at 72 ℃ for 10 minutes.
7. The method for preparing cannabis beer for pharmaceutical industry according to claim 1, wherein cold water is added in the sixth step, the temperature of wort is reduced to 75-80 ℃, and the contact time between wort and cannabis flowers and leaves for pharmaceutical industry is controlled to 8-10 minutes.
8. The method for preparing cannabis beer for pharmaceutical industry according to claim 1, wherein the time for the precipitation and whirling treatment in step seven is 20-40 minutes.
9. The method of claim 1, wherein the cell concentration of the inoculated fermentation broth in step eight is 600 to 1200 ten thousand cells/mL.
10. The preparation method of cannabis beer for pharmaceutical industry according to claim 1, wherein the time of the post-fermentation treatment in the ninth step is 12-15 days.
CN202011242146.9A 2020-11-09 2020-11-09 Preparation method of medical industrial hemp beer Pending CN112280626A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113604304A (en) * 2021-09-02 2021-11-05 吴达镕 Mabao sincere feeling beer and brewing method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张树林: "《医师药物手册 精神科》", 31 January 2008, 海洋出版社 *
黄韶清: "《常见中毒防与治》", 31 January 2005, 人民军医出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113604304A (en) * 2021-09-02 2021-11-05 吴达镕 Mabao sincere feeling beer and brewing method thereof

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