KR100678568B1 - Composition Comprising Bamboo Extract for Treating or Preventing Diabetes - Google Patents

Abstract

The present invention discloses a composition for treating or preventing diabetes comprising bamboo extract.

Bamboo Extract, Diabetes

Description

Composition Comprising Bamboo Extract for Treating or Preventing Diabetes

The present invention relates to a composition for treating or preventing diabetes comprising a bamboo extract.

Diabetes is a condition in which insulin, a glucose-regulating hormone secreted from the beta cells of the pancreas, is insufficient or does not function properly, causing blood glucose to accumulate in the blood instead of being used as energy, causing high blood sugar and detecting glucose in the urine.

Diabetes is usually divided into insulin dependent diabetes (type I diabetes) and insulin independent diabetes (type II diabetes). Insulin-dependent diabetes mellitus is caused by viral infections and is caused by the destruction of the beta cells of the pancreas, resulting in insulin secretion. It is also known as pediatric diabetes because it occurs mainly in young age groups of 10 to 20 years. The name is given because it is difficult to maintain life. Insulin-independent diabetes is caused by the lack of insulin in the pancreatic beta cells, but the amount is reduced and its action is caused, mainly after 30s, it is also called adult diabetes. Although it is called insulin-independent diabetes, it does not mean that insulin therapy is not necessary to treat hyperglycemia because it does not necessarily need to supply insulin from the outside to maintain life.

If diabetes persists for a long time, glucose is not properly absorbed into the body, and various metabolic disorders occur due to various metabolic disorders due to an imbalance in nutritional supply.

Diabetes complications include nerve complications caused by damage to nerve fibers and nerve membranes, diabetic retinopathy (non-proliferative retinopathy, proliferative retinopathy, diabetic cataracts), renal failure, sexual dysfunction, skin diseases (allergic), hypertension, atherosclerosis, stroke ( Stroke, heart disease (myocardial infarction, angina pectoris, heart attack), and gangrene.

Biguanides, thiazolidinediones, sulfonylureas, benzoic acid derivatives and α-glucosidase inhibitors are used to treat diabetes. Diabetes treatment with drugs has many side effects, and the World Health Organization (WHO) strongly recommends the use of natural products with fewer side effects. (Grover JK, Vats. V., Rathi. SS, Journal of Ethnopharmacology, 73 , pp 461-470, 2000).

Natural products reported to be effective in treating diabetes include moxibustion extracts (Korean Patent No. 464815), Apanaic acid derivative compounds isolated from Jangung Beetle Mushrooms (Korean Patent No. 457690), and extracts of hawthorn trees (Korean Patent No. 441787). , Polysaccharides isolated from Korean Angelica gigas (International Publication No. 2001-60386), bile of ruminant animals (US Patent No. 6645355) and the like.

The present invention also discloses a diabetic treatment or prevention effect of the natural bamboo extract.

An object of the present invention to provide a composition for treating or preventing diabetes comprising bamboo extract.

Other objects and specific aspects of the present invention will be presented below.

In one aspect, the present invention provides a composition for treating or preventing diabetes.

The present inventors induce high blood sugar by administering streptozotocin, a cytotoxic substance that destroys insulin-producing beta cells of the pancreas, to a laboratory animal, and then cut bamboo stems to the laboratory animal, When the bamboo stem was crushed in the form of sawdust and the bamboo extract obtained by adding water or ethanol as the extraction solvent to the pulverized product, it was confirmed that the blood sugar of the experimental animal is lowered.

Therefore, the composition for treating or preventing diabetes of the present invention is characterized in that it comprises a bamboo extract.

In the present specification, including the claims, the term "bamboo extract" means taxa regardless of the type of bamboo from which it is obtained, as well as its extraction method, as long as it has a diabetic therapeutic or prophylactic effect, specifically a hypoglycemic effect. It is defined as an extract of a solid phase or liquid phase obtained from the stem of a plant classified into.

Regardless of the type of bamboo, the bamboo extract is an extract obtained from bamboo belonging to Phyllostachys, an extract obtained from bamboo belonging to Arundinaria, an extract obtained from bamboo belonging to Sasa, And extracts obtained from bamboos belonging to Pseudosasa, and regardless of the extraction method, (i) extracts obtained by applying heat directly or indirectly to the shredded bamboo, and (ii) living Extracts in the form of a sperm obtained by cutting off the upper part of the bamboo and from the cut site; Extract obtained by extraction with nucleic acid, ethyl acetate, acetone, chloroform, methylene chloride or a mixed solvent thereof It is understood as a concept that includes all.

The bamboo extract used in the following examples of the present invention (bamboo extract of the reference example) is a bamboo extract obtained by the method of (ii) and bamboo extract obtained by the method of (iii), in particular, using water or ethanol as an extraction solvent. All of these bamboo extracts were shown to lower the hyperglycemia of the experimental animals induced by streptozotocin, although differences in their effects, as shown in the following examples.

These results can be said to predict that the other bamboo extract different from the extraction method will show similar results in that the bamboo extract used in the following examples of the present invention is obtained by different extraction methods.

Therefore, those skilled in the art will expect that bamboo extracts other than the bamboo extracts of the following reference examples also have a probable effect of treating or preventing diabetes, that is, hypoglycemic effect, within the range of their usual ability, based on the following examples of the present invention. Bamboo extracts with a hypoglycemic effect can be screened and identified within the usual capacity.

Therefore, even if the embodiment of the present invention confirmed the hypoglycemic effect with the bamboo extract of the reference example, the "bamboo extract" should be understood to include all the bamboo extract having a hypoglycemic effect in addition to the bamboo extract of the following reference example.

Nevertheless preferably the "bamboo extract" is understood as the bamboo extract of the following reference example, and more preferably as the bamboo extract obtained using water or ethanol in the extraction solvents listed above. More preferably, it is understood as a bamboo extract obtained using ethanol of at least 70% of the extraction solvent (% herein means volume percentage (v / v). Most preferably as a bamboo extract obtained using at least 80% ethanol. As shown in the following examples, the effect of enhancing blood sugar when using bamboo extracts obtained with 70% ethanol and 80% ethanol as the extraction solvent was 40% ethanol, 50% ethanol or 60% ethanol. This is because there was a sudden drop in blood pressure.

Above and below, including the claims, "70% or more ethanol" or "80% or more ethanol" can be understood as meaning any mixed solvent comprising ethanol in a concentration of 70% or more or 80% or more. The mixed solvent may be any mixture of two or more solvents if it comprises ethanol at a concentration of at least 70%. For example, a mixed solvent of water, acetone and ethanol also corresponds to 70% or more of ethanol as long as ethanol is contained in a concentration of 70% or more.

On the other hand, in the present specification, including the claims, "diabetes" is meant to include insulin-dependent diabetes and insulin-independent diabetes as described above, furthermore diabetes, such as thyroid function that occurs when the pancreas is damaged due to other diseases, etc. Diabetes mellitus, gestational diabetes mellitus due to hyperlipidemia, adrenal cortical hyperactivity, growth hormone hyperplasia or catecholamine hyperplasia.

In the composition for treating or preventing diabetes of the present invention, the bamboo extract as an active ingredient thereof may be included in any amount as long as it can exhibit the effect of treating or preventing diabetes or lowering blood sugar, preferably the total weight of the composition of the present invention. On the basis of the base may be included in more than 0.001% 99.999%.

In another aspect, the present invention provides a composition for treating or preventing diabetes complications comprising the bamboo extract.

Considering that it is generally necessary to reduce hyperglycemia to normal levels for the treatment or prevention of diabetic complications (Kim, Sang-Soo, Na Chang-Soo: Effect of Phenollic Compounds Extracted from Pear on Streptozotocin-induced Hyperglycemic Mice, Korean Journal of Food and Nutrition Science) , 31 (6), 1107-1111, 2002), the composition for treating or preventing diabetes complications of the present invention comprising as an active ingredient a bamboo extract exhibiting a hypoglycemic effect is used alone or for other diabetics for the treatment or prevention of diabetes complications. It can be used in combination with a therapeutic or prophylactic agent.

In the present specification, including the claims, the "diabetic complication" is meant to include all diseases secondary to the onset of diabetes, such as neurological complications, diabetic retinopathy, renal failure, Sexual dysfunction, skin diseases, high blood pressure, atherosclerosis, stroke, heart disease, gangrene and the like.

On the other hand, in the composition for treating or preventing diabetic complications of the present invention, the meaning of the bamboo extract, including the preferred embodiment thereof is effective as it is.

The composition for treating or preventing diabetes and the composition for treating or preventing diabetes complications of the present invention may be regarded as a pharmaceutical composition in a specific embodiment.

The pharmaceutical composition of the present invention may comprise a pharmaceutically acceptable carrier in addition to the bamboo extract as an active ingredient, such a pharmaceutically acceptable carrier is commonly used in pharmaceutical preparations, lactose, dextrose, water Cross, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydride Oxybenzoate, talc, magnesium stearate, mineral oil, and the like. The pharmaceutical compositions of the present invention may also further comprise lubricants, wetting agents, sweeteners, flavoring agents, emulsifiers, suspending agents, preservatives and the like as additives.

The carrier may be included in the pharmaceutical composition of the present invention in an amount of about 1% by weight to about 99.999% by weight, preferably about 50% by weight to about 99.999% by weight, with the additive being about 0.1% by weight. To about 20% by weight.

On the other hand, the pharmaceutical composition of the present invention can be administered by various routes, for example, orally or rectally, or by injection into veins, muscles, subcutaneous, intrauterine dural, and the like.

The pharmaceutical compositions of the present invention may be prepared in unit dose form or formulated using pharmaceutically acceptable carriers and / or excipients or incorporated into multi-dose containers. In this case, the formulation may be in the form of a solution, a suspension or an emulsion, or may include an exercire, an excipient, a powder, a granule, a tablet, a warning, a coughing agent, a lotion, an ointment, and the like.

The daily dosage of the pharmaceutical composition of the present invention is usually 0.001 ~ 150 mg / kg body weight range, it can be administered once or divided into several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage limits the scope of the present invention in any aspect. It should not be understood as.

The composition for treating or preventing diabetes and the composition for treating or preventing diabetes complications of the present invention may be regarded as a food composition in a specific embodiment.

The food composition of the present invention may be prepared from gums, vitamin complexes, dietary supplements, special nutritional supplements, functional drinks, and the like.

In addition to the bamboo extract is included in the food composition of the present invention, glucose, monosaccharides such as fructose, disaccharides such as maltose, sucrose, or polysaccharides such as dextrin, cyclodextrin, and the like, and xylitol, sorbitol, erythritol, etc. Sugar alcohols may also be added, sweeteners such as taurine, stevia extract, and the like may also be added, and other food additives may also be added.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

<Reference Example> Preparation of Bamboo Extract

Reference Example 1 Preparation Example 1 of Bamboo Extract

The stems of three-year-old bamboo shoots, which live in Damyang-gun, Jeollanam-do, were collected in May 2004, washed with water, dried and used as samples. First, prepare the bamboo sawdust by pulverizing the dried sample, and then put the bamboo sawdust into a 2000ml round flask and add about 5-10 times the bamboo sawdust (based on the weight of bamboo sawdust) for 3 hours at 95 ℃. To the extent extracted. The extract was concentrated again with a vacuum concentrator (Buchi Co. R-114, Germany) and then lyophilized to prepare a solid bamboo extract.

Reference Example 2 Preparation Example 2 of Bamboo Extract

Bamboo extracts were prepared in the same manner as in <Reference Example 1>, except that the three-year-old cotton rod stems collected in October 2004 were used instead of the stems of the bamboo shoots collected in May 2004.

Reference Example 3 Preparation Example 3 of Bamboo Extract

The stems of three-year-old bamboo shoots, which live in Damyang-gun, Jeollanam-do, were collected in May 2004, washed, dried, and crushed to prepare bamboo sawdust. Put a bamboo sawdust into a 2000 ml round flask, and add 40% of ethanol (a mixed solvent of water and ethanol; same as below) about 10 times of bamboo sawdust (based on the weight of bamboo sawdust). The phases were extracted for 3 hours and then filtered over Whatman NO.5A. The extract was concentrated again with a vacuum concentrator (Buchi Co. R-114, Germany) and then lyophilized to prepare a solid bamboo extract.

Reference Example 4 Preparation Example 4 of Bamboo Extract

Bamboo extract was prepared in the same manner as in <Reference Example 3>, except that 50% of ethanol was used instead of 40% of ethanol.

Reference Example 5 Preparation Example 5 of Bamboo Extract

Bamboo extract was prepared in the same manner as in <Reference Example 3>, except that 60% ethanol was used instead of 40% ethanol as the extraction solvent.

Reference Example 6 Preparation Example 6 of Bamboo Extract

Bamboo extract was prepared in the same manner as in <Reference Example 3>, except that 70% ethanol was used instead of 40% ethanol as the extraction solvent.

Reference Example 7 Preparation Example 7 of Bamboo Extract

Bamboo extract was prepared in the same manner as in <Reference Example 3>, except that 80% of ethanol was used instead of 40% of ethanol.

Reference Example 8 Preparation Example 8 of Bamboo Extract

Bamboo extract was prepared in the same manner as in <Reference Example 3>, except that the three-year-old cotton rod stem obtained in October 2004 was used instead of the stem of the bamboo shoots collected in May 2004.

Reference Example 9 Preparation Example 9 of Bamboo Extract

Bamboo extracts were prepared in the same manner as in <Reference Example 4>, except that three-year-old cotton rod stems collected in October 2004 were used instead of the stems of the bamboo shoots collected in May 2004.

Reference Example 10 Preparation Example 10 of Bamboo Extract

Bamboo extracts were prepared in the same manner as in <Reference Example 5>, except that the three-year-old cotton rod stems collected in October 2004 were used instead of the stems of the bamboo shoots collected in May 2004.

Reference Example 11 Preparation Example 11 of Bamboo Extract

Bamboo extracts were prepared in the same manner as in <Reference Example 6>, except that three-year-old cotton rod stems collected in October 2004 were used instead of the stems of the bamboo shoots collected in May 2004.

Reference Example 12 Preparation Example 12 of Bamboo Extract

Bamboo extracts were prepared in the same manner as in <Reference Example 7>, except that the three-year-old cotton rod stems collected in October 2004 were used instead of the stems of the bamboo shoots collected in May 2004.

Reference Example 13 Preparation Example 13 of Bamboo Extract

In May 2004, bamboo stems of three-year-old bamboo shoots were cut to recover the essences extracted from the cut portions, and the extracts of the essence form were lyophilized to obtain solid extracts.

Reference Example 14 Preparation Example 14 of Bamboo Extract

In October 2004, three-year-old bamboo stems were cut to recover the essences extracted from the cut portions, and the extracts in the form of the essences were lyophilized to obtain solid extracts.

<Example> Evaluation of Antidiabetic Effect of Bamboo Extract

<1> experimental animals

Experimental animals purchased male rats (ICR strain) weighing about 250g from Damul Science (Daejeon, Korea) and supplied solid feed (Samyangsa, Korea) and water at Dongshin University (Gwangju, Gwangju) for more than 1 week. Used after adapting to laboratory environment.

<2> hyperglycemia in experimental animals

Hyperglycemia induced by fasting the animals for 12 hours and then intraperitoneally administered 50 mg / kg of streptozotocin (N- [methylnitrosocarbamoyl] -D-glucosamine or less "STZ",) at 24 hour intervals 3 After 1 day, STZ 100 mg / kg was intraperitoneally administered to induce hyperglycemic mice. Fasting blood glucose of normal experimental animals at about 70 mg / dL was about 113 mg / dL after hyperglycemia was used in the experiment.

<3> Administration of Bamboo Extract of Reference Example

Bamboo extract of <Reference Example> was orally administered once a week for 2.0 weeks / 2.0mg / kg to each of the hyperglycemic-induced experimental animals. On the other hand, the experimental animals as a control group was administered with saline instead of bamboo extract.

<4> blood sugar measurement

Blood glucose was measured once a week for 6 weeks, fasted 12 hours before measurement, and blood was collected from the tail vein and measured by Glucometer 4 (Bayer Co., USA).

<5> results

The results are shown in Table 1 below.

Changes in blood sugar division Week 0 1 week 2 weeks 3 weeks 4 Weeks 5 Weeks 6 Weeks Control group (physiological saline) 113 140 293 272 265 242 324 Reference Example 1 113 138 147 173 164 153 156 Reference Example 2 113 142 146 163 172 162 167 Reference Example 3 113 142 194 232 221 195 201 Reference Example 4 113 147 267 242 234 205 197 Reference Example 5 113 151 253 211 198 204 191 Reference Example 6 113 132 146 162 153 146 139 Reference Example 7 113 128 148 144 147 138 123 Reference Example 8 113 151 172 254 267 243 242 Reference Example 9 113 148 265 268 259 232 239 Reference Example 10 113 153 211 253 278 222 237 Reference Example 11 113 147 185 196 211 189 173 Reference Example 12 113 151 167 158 162 153 147 Reference Example 13 113 172 287 279 293 269 272 Reference Example 14 113 141 296 285 284 275 282

* In Table 1, each value is a measured blood glucose level (mg / dL).

As shown in Table 1, all of the bamboo extracts of the reference example show anti-diabetic efficacy. The anti-diabetic effect was higher for bamboo extracts obtained by using an extraction solvent than bamboo extracts obtained by the direct sampling method, and especially when 70% or 80% ethanol was used as the extraction solvent. Of particular note, when using ethanol as the extraction solvent, the antidiabetic effect increased gradually as the concentration of ethanol increased, and the effect increased rapidly in the case of 70% and 80% ethanol.

<Example Formulation Examples of Pharmaceutical Compositions and Food Compositions

Preparation Example 1 Preparation of Capsule

5 g of the bamboo extract of <Reference Example 6>, 100 mg of corn starch, 100 mg of lactose, and 2 mg of magnesium stearate were completely mixed, and then filled into hard gelatin capsules according to a conventional method for preparing capsules.

Preparation Example 2 Preparation of Injection

5 g of the bamboo extract of <Reference Example 6> using a suitable amount of distilled water for injection and a pH adjusting agent, the bamboo extract was dissolved in distilled water for injection and adjusted to a pH of about 7.5 according to a conventional method for preparing an injection. Injections were prepared by filling a 2 ml ampoule with distilled water and sterilizing.

Preparation Example 3 Preparation of Functional Drink

Instant sterilization by homogeneously combining 5 g of bamboo extract of <Reference Example 6> with subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%) and water (75%) After this, it was packaged in small packaging containers such as glass bottles and plastic bottles to prepare a healthy beverage.

As described above, the present invention provides a composition for treating or preventing diabetes and a composition for treating or preventing diabetes complications.

The compositions of the present invention can be applied and used as pharmaceutical compositions or food compositions.

Claims (11)

A composition for treating or preventing diabetes comprising bamboo extract obtained by extracting bamboo stems with an aqueous ethanol solution of 70% or more. The method of claim 1, The composition is a composition for treating or preventing diabetes, characterized in that the pharmaceutical composition. The method of claim 1, The composition is a composition for treating or preventing diabetes, characterized in that the health functional food composition. delete The method according to any one of claims 1 to 3, The bamboo extract is a composition for treating or preventing diabetes, characterized in that obtained by extracting bamboo stems with an aqueous solution of 80% or more. delete delete delete delete delete delete