KR101907179B1 - Method for production of sulforaphene-enriched raphanus sativus seeds extracts and Food composition, pharmaceutical composition, animal medicines for weight and blood glucose control, fatty liver prevention with the raphanus sativus seeds extracts therefrom - Google Patents

Abstract

The present invention relates to a method for manufacturing a radish seed extract containing a large amount of sulforaphene, a radish seed extract obtained therefrom and a composition for preventing or treating obesity, diabetes, and fatty liver containing the radish seed extract. The addition of 5-40% (v/v) ethanol as an extraction solvent to the pulverized product of radish seed can be used to extract sulforaphene from the radish seed by high yield, and the manufacturing process is simple and stable to be applied in the food industry. In addition, a food composition, a pharmaceutical composition and an animal medicine for ameliorating, preventing, and treating obesity, diabetes, and fatty liver containing the ethanol extract of radish seed of 5-40% (v/v) of the present invention exhibit more excellent effects of ameliorating, preventing, and treating obesity, diabetes, and fatty liver by extracting various active ingredients from the radish seed and the compound synergy effect.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for producing a radish extract containing a large amount of sulforaphin and a method for producing a sulforaphane-enriched raphanus sativus seeds extracts and food composition, pharmaceutical composition, animal medicines for weight and blood glucose control, fatty liver prevention with the raphanus sativus seeds extracts therefrom}

The present invention relates to a method for preparing a radish extract containing a large amount of sulfo-fins and a composition for improving, preventing and treating obesity, diabetes and fatty liver, which contains the radish extract extracted therefrom. More specifically, The present invention relates to a method for producing a radish extract containing 5 to 40% (v / v) ethanol and a large amount of sulfolapin, and a method for producing obesity, diabetes mellitus, A food composition for improving, preventing, and treating fatty liver, a pharmaceutical composition, and an animal medicine.

Due to westernized eating habits and a decrease in exercise, the proportion of obese people is rapidly increasing worldwide. Obesity and overweight are widespread worldwide and are known to be overweight in at least 20% of the population (US, UK, France, Germany, Italy, Spain and Japan) and up to 45% in many. Since obesity is a major cause of metabolic diseases such as hyperlipidemia, diabetes and fatty liver as well as its own problems, controlling body weight is very important for maintaining health. Many obesity drugs developed to date have a mechanism of acting on the digestive system or the nervous system, and many side effects have been reported, and most of them are forbidden on the market. Because of concerns about the safety of therapeutic agents, studies have been actively conducted to prevent or treat obesity as an active ingredient derived from natural products.

Obesity, on the other hand, is an important risk factor for type 2 diabetes, and the incidence of diabetes varies according to the degree of obesity, duration of obesity, and distribution of body fat. It is reported that the incidence of diabetes increases with the increase of the body mass index, and the incidence of diabetes is 40 times higher in people with a BMI of 35 ㎏ / ㎡ than those with a body mass index of 23 ㎏ / ㎡. However, existing diabetes drugs have side effects that cause gastrointestinal disorders, hypoglycemia, edema, etc., and it is necessary to develop a new concept of therapeutic drugs because of the disadvantage that the drug administration method is injectable and has weak drug effect.

On the other hand, obesity and type 2 diabetes are major diseases directly related to the development of liver disease. Fatty liver is a case of excessive accumulation of triglyceride in liver tissue. It is called nonalcoholic fatty liver disease when it is caused by metabolic abnormality such as obesity or diabetes. To date, weight loss has been a priority for effective treatment of diabetes mellitus. Metformin, thiazolidinedione, and statin-based anticoagulants have been shown to improve symptoms However, there is no drug that can be used effectively and safely for a long period of time.

On the other hand, it is known that Mucus (abdomen) has the effect of stopping the abdominal cavity, trimming, gastric hyperplasia, diarrhea, seawater, asthma, constipation, anorexia, old skin and old cough. Pharmacological actions include inhibition of staphylococci Action, inhibition of skin fungi, hypotensive effect, anti-inflammatory action, etc. have been reported, and have been used for a long time as a main ingredient of Taeum Jowe-tang, a Chinese medicine for controlling body weight. As the indicator components of this kind of Si, sulfolapine, eruic acid, linoleic acid, glycerin locustic ester and cinchin are known.

On the other hand, Pengqun Kuang (2013) and Kuang P1 (2013) describe the separation of sulfo-fins from mushrooms. Since all of the columns are used to separate sulforaphane from mushrooms, (Pengqun Kuang, Food Chemistry 136 (2013) 342-347, Preparative Separation and Purification of Sulforaphene from Sulforaphene from Preparative Separation and Purification of Sulforaphane from Radish Seeds using Macroporous Resin and Preparative High-Performance Liquid Chromatography, radish seeds by high-speed countercurrent chromatography, Kuang P1, Food Chem, 2013 Jan 15; 136 (2): 309-15.).

In addition, Korean Patent Nos. 10-1028865 and 10-1028864 disclose that the supernatant obtained by centrifuging the hydrolyzate of radish hydrolyzate or radish hydrolyzate is subjected to liquid-liquid extraction or solid-phase extraction, A method for separating sulforaphane from a sulforaphane is disclosed. However, this is also a complicated process, and is a method of extracting sulfolane from non-mucilage or non-mucilage.

It is an object of the present invention to provide a method for preparing a radish extract which can be efficiently extracted from mucus and can be applied to the food industry by a safe and simple process and a radish extract obtained therefrom.

It is still another object of the present invention to provide a food composition, a pharmaceutical composition, and an animal medicine for obesity, diabetes, fatty liver improvement, prevention and treatment, containing 5 to 40% (v / v) ethanol extract of Cordyria.

In order to achieve the above object, the present invention provides a method for preparing a radish extract, which comprises extracting 5 to 40% (v / v) ethanol as an extraction solvent in a first form of the present invention.

In the present invention, the extraction may be preferably an immersion extraction.

In the present invention, the extraction solvent may preferably be 5-10% (v / v) ethanol.

In the present invention, the extraction may preferably be performed at a temperature of 35 to 45 DEG C for 10 to 15 hours.

Meanwhile, the second aspect of the present invention provides a radish extract having an increased sulfolane content, which is extracted by adding 5 to 40% (v / v) ethanol as an extraction solvent to the radish.

The sulfolane content of the radish extract with increased sulfolane content may preferably be at least 18.36 mg as compared to 1 g of the total solids in the extract.

Meanwhile, the third aspect of the present invention is a food composition for improving obesity containing an extract obtained by adding 5 to 40% (v / v) ethanol as an extraction solvent to an alcoholic beverage, a pharmaceutical composition for preventing or treating obesity, An animal pharmaceutical composition is provided.

The present invention also provides a food composition for improving diabetes, comprising a extract obtained by adding 5 to 40% (v / v) ethanol as an extraction solvent to an alcoholic beverage, a pharmaceutical composition for preventing or treating diabetes, An animal pharmaceutical composition is provided.

The fifth aspect of the present invention is a food composition for improving fatty liver comprising an extract obtained by adding 5 to 40% (v / v) ethanol as an extraction solvent to an alcoholic beverage, a pharmaceutical composition for prevention or treatment of fatty liver, An animal pharmaceutical composition is provided.

The present invention can extract sulforaphin in high yield from mucilage using 5 ~ 40% (v / v) ethanol as an extraction solvent, and is easy to manufacture and has excellent stability in the food industry. It is possible.

In addition, the present invention can provide a composition exhibiting excellent obesity, diabetes, fatty liver improvement, prevention, and therapeutic effect by containing extract extracted from 5 to 40% (v / v) ethanol as an extraction solvent . The 5 to 40% (v / v) ethanol extract of the present invention extracts various active ingredients from mucilaginus, and exhibits a more excellent effect of obesity, diabetes, fatty liver improvement, prevention and treatment by its complex synergistic effect.

Figure 1 is a sulforapin standard and a standard curve. 'SFEN' means sulfolapin.
FIG. 2 shows the results of measuring the weight of the extract, the concentration of the sulfolane in the extract, and the weight of the total sulfolane according to the solvent conditions.
FIG. 3 shows the results of confirming the change in body weight gain due to the addition of 10% fermented alcoholic extract of Rumex.
FIG. 4 shows the results of the feed intake according to the feeding of 10% fermented mugwort extract.
FIG. 5 shows the results of confirming fasting blood glucose changes according to the feeding of 10% fermented alcoholic beverage extract.
FIG. 6 shows the results of confirming changes in fasting insulin according to the feeding of 10% fermented mugwort extract.
FIG. 7 shows the results of confirming the insulin sensitivity improving effect of the 10% fermented mugwort seed extract.
FIG. 8 shows the results of confirming the effect of controlling the blood glucose level by feeding 10% fermented mugwort extract.
FIG. 9 shows the result of calculating the blood glucose curve backward time after feeding 10% fermented mugwort extract to rats.
FIG. 10 shows the result of liver weight change according to the addition of 10% fermented mugwort extract.
FIG. 11 shows the results of confirming the effect of the 10% fermented mugwort extract on liver lipid reduction.
FIG. 12 shows the results of confirming the intra-liver AST level according to the 10% fermented alcoholic beverage extract.
FIG. 13 shows the results of confirming the level of ALT in liver according to the feeding of 10% fermented alcoholic beverages.
FIG. 14 shows the result of measurement of the amount of liver lipid according to the feeding of the 10% fermented alcoholic extract.
FIG. 15 shows the results of measurement of the total amount of triglyceride in liver according to the feeding of 10% fermented alcoholic extract of fermented rice.
FIG. 16 shows the results of measurement of the total cholesterol content in the liver according to the feeding of 10% fermented alcoholic extract of fermented rice.

The inventors of the present invention have found that glucosinolate components are present in cruciferous plants such as mucilage (oboe) and rhizome, and phytochemicals such as 4-methylsulfonyl- It was confirmed that 4-methylsulfinyl-3-butenyl isothiocyanate or 4-methylsulfonyl-3-butenyl isothiocyanate precursor or derivative was effective for preventing obesity 10-1213065).

Accordingly, the inventors of the present invention have paid attention to sulforaphene (SFEN), which is a kind of glucosinolate. In the present invention, it is possible to efficiently produce sulforaphane from a rice bran Process.

Conventionally, there is only a technology for simply separating sulforaphin from mucus, a technique for extracting sulforaphin from a mucilage at a high yield, and a simple and safe process that can be applied to the food industry have not been disclosed.

In accordance with the present invention, a radish extract containing a large amount of sulfolapin can be obtained by adding 5 to 40% (v / v) ethanol to an extractor as an extraction solvent. Especially, when 5 ~ 10% (v / v) ethanol is used as the extraction solvent, the amount of sulfolapin to be extracted is remarkably high. According to the following examples, when an aqueous solution of ethanol, water or ethyl acetate deviating from the above range is used as an extraction solvent, sulfolapin is hardly extracted or the extraction amount is very low.

At this time, the ethanol may include anhydrous ethanol and fermented alcohol, and in the case of producing the edible alcohol, ethanol is preferably a fermentation alcohol.

On the other hand, the above extraction is preferably an immersion extraction. Since the activity of myosinase in Mo is limited during the extraction of hot water, the extraction method without elevation of temperature is used.

The reflux extraction method is generally used for preparing edible extracts, but the reflux extraction method can increase the temperature of the solvent during extraction and complicate the process compared to the immersion extraction.

The extraction is preferably carried out at a temperature of 35 to 45 DEG C for 10 to 15 hours. If the temperature is lower than 35 ° C, the sulfo-fins can not be extracted sufficiently. If the temperature exceeds 45 ° C, the action of myrosinase (activity temperature: 37-42 ° C) That is, when the temperature is above the extraction temperature, it is not possible to produce an extract of Alnus japonica containing a large amount of sulfolapin.

 Meanwhile, the present invention provides a radish extract having increased sulforaphin content obtained by adding 5 to 40% (v / v) ethanol as an extraction solvent to pulverized muffins. The radish extract of the present invention contains at least 18.36 mg of sulforaphin per gram of the total solids of the extract. Since the radish extract of the present invention uses ethanol as an extraction solvent, it can be used directly as a food material. The thus obtained radish extract contains a large amount of sulfolapine, and thus various pharmacological effects can be expected.

Meanwhile, the present invention provides a food composition, a pharmaceutical composition, or an animal medicine for obesity, diabetes, fatty liver improvement, prevention and treatment, containing 5 to 40% (v / v) ethanol extract of SiC.

In the past, Moo (underwear) has been mainly used as a component of Chinese herb medicine called Taeum Joywantang, and has been taken in the form of hot water extract of Mujung. However, the inventors of the present invention have found that an extract of 5 to 40% (v / v) ethanol aqueous solution as an extraction solvent, rather than a hot-water extract of Succulent extract, further extracts various active ingredients from the sucrose, , Diabetes, fatty liver improvement, prevention and treatment effects were revealed.

At this time, the 5 to 40% (v / v) ethanol extract of the present invention can be extracted by a method commonly used in the technical field of the present invention, but preferably 5 to 40% (v / v) ethanol And then extracted at a temperature of 35 to 45 ° C for 10 to 15 hours.

In addition, the ethanol may include anhydrous ethanol, a fermented alcohol.

In addition, the fatty liver may be a non-alcoholic fatty liver caused by ingestion of a high fat diet.

Meanwhile, the food composition of the present invention is characterized in that it contains 5 ~ 40% (v / v) ethanol extract of Si-Cu, and the extract is preferably 0.000001 ~ 50 wt% of the food composition. When the amount is less than 0.000001% by weight, the effect is insufficient. When the amount is more than 50% by weight, the increase in the effect on the usage amount is insignificant, which is uneconomical.

On the other hand, the food composition of the present invention is preferably used in foods, cereals, caffeinated beverages, general drinks, chocolate, breads, And supplementary foods. However, the present invention is not limited thereto.

Meanwhile, the pharmaceutical composition or the veterinary pharmaceutical composition of the present invention is characterized in that it contains 5 ~ 40% (v / v) ethanol extract of Si-Ca, It is preferable to control it according to the type of disease and the severity of the disease. In the pharmaceutical composition or the veterinary pharmaceutical composition of the present invention, the content of the radish extract may be 0.000001 to 50% by weight in the pharmaceutical composition or the veterinary pharmaceutical composition, but is not limited thereto. However, when the content is less than 0.000001% by weight, the effect may be insufficient. When the content is more than 50% by weight, the rate of increase of the effect relative to the amount of use may be low.

Meanwhile, the pharmaceutical composition or the animal pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier, diluent or excipient in addition to the active ingredient. Examples of usable carriers, excipients or diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, Microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. These may be used singly or in combination. When the preventive and therapeutic agent is a pharmaceutical agent, a filler, an anticoagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent or an antiseptic agent may be additionally included.

On the other hand, the formulation of the pharmaceutical composition or the veterinary medicament of the present invention may be in a desirable form depending on the method of use, and may be a method known in the art so as to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal To be formulated. Examples of specific formulations include PLASTERS, GRANULES, LPTIONS, LINIMENTS, LEMONADES, AROMATIC WATERS, POWDERS, Syrups SYRUPS, OPHTALMIC OINTMENTS, LIQUIDS AND SOLUTIONS, AEROSOLS, EXTRACTS, ELIXIRS, OINTMENTS, FLUIDEXTRACTS, EMULSIONS, ), Suspensions, DECOCTIONS, INFUSIONS, OPHTHALMIC SOLUTIONS, TABLETS, SUPPOSITIORIES, INJECTIONS, SPIRITS, CATAPLSMA, ), Capsules, CREAMS, TROCHES, TINCTURES, PASTES, PILLS, soft or hard gelatin capsules.

On the other hand, the dosage of the pharmaceutical composition or the animal pharmaceutical composition of the present invention is preferably determined in consideration of the administration method, the age, sex and weight of the recipient (animal), severity of the disease, and the like. For example, the pharmaceutical composition for preventing or treating obesity of the present invention can be administered at least once at a dose of 0.1 to 100 mg / kg (body weight) per day based on the active ingredient. However, the above dosage is merely an example and can be changed by a physician's prescription depending on the condition of the recipient (animal).

Hereinafter, the contents of the present invention will be described in more detail in the following examples, but the scope of the present invention is not limited to the following examples, but includes modifications of equivalent technical ideas.

[ Example  One: Sulfolafine  Manufacture of a large amount of radish extract]

11 kinds of solvents were prepared by using water, ethanol (fermented alcohol) and ethyl acetate (0% fermented alcohol, 2% fermented alcohol, 5% fermented alcohol, 30% Fermented Spirits, 40% Fermented Spirits, 50% Fermented Spirits, 70% Fermented Spirits, 30% Fermented Spirits, 10% Ethyl Acetate, 30% Fermented Spirits, 50% Acetic Acid Ethyl ', 100% ethyl acetate).

800 ml of each solvent was added to 100 g of finely ground Mucilage (innerwear) using a blender, and the mixture was immersed and extracted at a temperature of 40 캜 for 12 hours to obtain a radish extract. After that, each of the Ricinus extracts was concentrated and lyophilized to a powder form.

[ Experimental Example  1: Extraction yield of radish extract and extracts of radish extract Sulfolapin  Content measurement]

The extraction yield of each of the radish extracts extracted by solvent and the content of sulforaphane in the radish extract were measured by the above Example 1.

The weight of each extract was measured and the extraction yield was calculated against the raw material.

 10 mg of each of the radish extracts was dissolved in 1 ml of a 60% ethanol solution, and the solution was filtered through a 0.45 μm syringe filter to prepare a test solution. Sulfolapin standards were prepared at various concentrations (25 ppm, 50 ppm, 125 ppm, 250 ppm, 500 ppm) to prepare standard solutions. The content of sulfolane in the test solution and the standard solution prepared above was quantitatively determined using an HPLC machine, and an XDB-C18 column was used. The liquid phase was a mixed solution of water, phosphoric acid solution and acetonitrile, and signals were detected at 215 nm. Thereafter, the relation between the sulfolane concentration and peak area of the standard solution was determined, and the peak area obtained by analyzing the test solution was substituted into the relational expression to calculate the sulfolane concentration of the test solution (see FIG. 1). Figure 1 is a sulforapin standard and a standard curve. 'SFEN' means sulfolapin.

Also, the total yield of sulfolapin extracted from the same amount of Sucralose (underwear) was calculated by multiplying the extraction yield by the concentration of sulfolapin in the extract.

The measurement results are shown in Fig. FIG. 2 shows the results of measuring the weight of the extract, the concentration of the sulfolane in the extract, and the weight of the total sulfolane according to the solvent conditions.

As can be seen in FIG. 2, the total sulfolane weight of the 5 to 40% fermented mugwort seed extract was 190 mg or more, indicating that the total sulforaphane weight was significantly higher than other conditions. Especially, it was confirmed that the weight of extract, the concentration of sulfapyrin in the extract, and the weight of total sulforaphane were high in the 5 to 10% fermentation alcohol solution.

[ Experimental Example  2: Checking the efficacy of ethanol extract of mucilage on weight control]

In this experimental example, the effect of controlling the weight of the ethanol extract of sucrose was confirmed.

In the present invention, an experiment was conducted to compare the effect of ethanol extract of sucrose on body weight. Control diet (CD), high fat diet (HFD), high fat diet + 10% fermented mushroom extract 0.3% (HFD + RSEL) and high fat diet +10 % Of fermented fermented mushroom extract (HFD + RSEH) ', and when the feed is changed, the amount of feed remaining after ingestion is measured to calculate the feed intake And weekly weight changes were observed.

As a result, the rats fed with the general diet showed a weight increase of about 8 g for 16 weeks and the rats fed the high fat diet alone increased the weight of 20 g, which is about 2.5 times the weight gain of the rats fed the normal diet, The mice fed the 10% fermented mugwort extract showed significantly lower weight gain than rats fed the high fat diet alone (Fig. 3). FIG. 3 shows the results of confirming the change in body weight gain due to the addition of 10% fermented mugwort extract.

On the other hand, there was no significant difference in the amount of feed intake between the 10% fermented fermented milk extract (Fig. 4). FIG. 4 shows the results of the feed intake according to the feeding of 10% fermented mugwort extract.

From the results, it was found that the 10% fermented mugwort extract significantly alleviated the weight gain due to the high fat diet through a mechanism different from that of the existing obesity treatment inhibiting the appetite.

[ Experimental Example  3: Effect of ethanol extract of sucrose on fasting plasma glucose, fasting insulin and insulin sensitivity]

In this experiment, the effects of ethanol extract of sucrose on fasting plasma glucose, fasting insulin and insulin sensitivity were examined.

Control diet (CD), high fat diet (HFD), high fat diet + 10% fermented mushroom extract 0.3% (HFD + RSEL) and high fat diet +10 % Fasting glucose and 1.5% (HFD + RSEH) 'in fasting state. Fasting blood glucose and fasting insulin were measured in fasted state. Thereafter, the HOMA IR (homeostatic model assessment) was measured, and the HOMA-IR calculation was performed as shown in the following equation (1).

[Equation 1]

HOMA IR = fasting insulin (μU / ml) × fasting glucose (mg / dL) / 405

As a result, fasting blood glucose level was 1.5 times higher in rats fed high fat diets than high fat diet fed rats, and fasting blood glucose levels in rats fed with 10% (Fig. 5). As shown in Fig. FIG. 5 shows the results of confirming fasting blood glucose changes according to the feeding of 10% fermented alcoholic beverage extract.

In addition, the fasting insulin of the rats fed the 10% fermented mugwort extract also showed a tendency to decrease compared with the rats fed the high fat diet alone (Fig. 6). FIG. 6 shows the results of confirming changes in fasting insulin according to the feeding of 10% fermented mugwort extract.

In addition, as a result of the HOMA IR measurement, it was confirmed that the high-fat diet improves the insulin sensitivity significantly in the high-dose group of 10% fermented rumen extract (FIG. 7). FIG. 7 shows the results of confirming the insulin sensitivity improving effect of the 10% fermented mugwort seed extract.

[ Experimental Example  4: Confirmation of blood sugar-improving effect of ethanol extract of mucilage]

In this experimental example, the effect of improving the blood glucose level of the ethanol extract of sucrose was confirmed.

The control diet (CD), high fat diet (HFD), high fat diet + 10% fermented mushroom extract 0.3% (HFD + RSEL) and high fat diet +10 % Fermented mugwort extract (HFD + RSEH) ', and then starved for 16 hours.

A dose of 2 g / kg b.w. of glucose was injected into the abdominal cavity of the rats. The area under curve was calculated from 0 to 120 minutes.

As a result, the rats fed with the normal diet showed a slight increase in blood glucose level but fell off immediately. However, the rats fed the high fat diet had significantly higher blood glucose levels than the rats fed the normal diet, In addition, the rats fed the 10% fermented mugwort extract showed a smaller increase in blood glucose and a lower rate of blood glucose lowering than rats fed the high fat diet (FIGS. 8 and 9). FIG. 8 is a graph showing the effect of the 10% fermented fermented mugwort extract on the blood glucose control effect, and FIG. 9 is a graph showing the blood glucose curves obtained after feeding 10% fermented mugwort extract to rats.

[ Experimental Example  5: Ethanol extract of mucilage Non-alcoholic  Checking the efficacy of fatty liver improvement]

In this experimental example, the non-alcoholic fatty liver improvement effect caused by the ingestion of the high-fat type ethanol-ethanol extract was confirmed.

Control diet (CD), high fat diet (HFD), high fat diet + 10% fermented mushroom extract 0.3% (HFD + RSEL) and high fat diet +10 % HFD + RSEH ', and then liver was extracted from each rat.

(1) Liver weighing

As a result of liver weighing, the liver weight of the rats fed the high fat diet alone was significantly higher than that of the rats fed the normal diet, and the liver weight of the rats fed the 10% And the liver weight of the mice fed alone was significantly lower than the liver weight of the fed mice (Fig. 10). FIG. 10 shows the result of liver weight change according to the addition of 10% fermented mugwort extract.

(2) Confirming the effect of reducing the fat in the liver

For histologic examination, the liver was immediately fixed in 4% formalin solution, and then paraffin block was prepared by washing, dehydrating, transparency, infiltration and embedding process. The prepared block was sectioned and stained with H & E (hematoxylin-eosin) and observed with an optical microscope.

As a result of H & E staining of liver tissues, fat accumulation was observed in the high fat diet group, but fat fat accumulation in the liver was remarkably decreased when 10% fermented milk fat extract was fed ( 11). FIG. 11 shows the results of confirming the effect of the 10% fermented mugwort extract on liver lipid reduction.

(3) Analysis of serum biochemical indicators

The AST and alanine aminotransferase (ALT) levels were significantly lower in the AST and ALT levels compared to the high - fat diet group. (Fig. 12, Fig. 13). FIG. 12 shows the results of confirming the AST levels in the liver according to the treatment with 10% fermented alcoholic beverages and FIG. 13 shows the levels of ALT in the liver according to the treatment with 10% fermented alcoholic beverages.

(4) lipid analysis of liver tissue

For lipid analysis of hepatic tissues, 1 ml of 3 M CaCl ₂ solution was added to 250 mg of liver tissue, and homogenized with a homogenizer. Folch solution (chloroform: methanol mixture (2: 1, v / v) The lipids were extracted from the hood by thoroughly draining the organic solvent, and the remaining pure lipid was treated with 1 ml of a mixture of triton: ethanol (1: 3, v / v) Were used. The total triglyceride (TG) and total cholesterol (TC) of liver tissues were analyzed by using a commercial kit for TG and TC measurement, respectively.

Liver lipid, total triglyceride, total cholesterol, liver lipid, triglyceride, and total cholesterol were significantly higher in the rats fed the 10% (Figs. 14 to 16).

FIG. 14 shows the result of measuring the amount of liver lipid according to the feeding of the 10% fermented fermented mugwort extract, FIG. 15 shows the result of measuring the total amount of triglyceride in the liver according to the feeding of 10% fermented fermented mugwort extract, Is the result of measuring the total cholesterol content of liver according to the feeding of 10% fermented mugwort extract.

[Formulation Example 1: Preparation of food composition for obesity improvement]

In the present formulation example, an obesity-improving food composition containing the 10% (v / v) fermented mugwort seed extract prepared in Example 1 was prepared as follows.

(1) biscuit manufacture

By weight of starch, 0.77% by weight of glucose, 0.78% by weight of glucose, 11.78% by weight of palm shortening, 1.54% by weight of ammonia, 0.17% by weight of sodium bicarbonate, 0.16% by weight of sodium bisulfite 1.45% by weight of rice flour, 0.0001% by weight of vitamin B1, 0.0001% by weight of vitamin B2, 0.04% by weight of milk fractions, 20.6998% by weight of water, 1.16% by weight of whole milk powder, 0.29% by weight of substitute milk powder, 0.29% by weight of salt and 7.27% by weight of spray oil and 1% by weight of the extract of Radix seed were mixed to prepare a biscuit by a conventional method.

(2) Health drinks manufacturing

0.2% by weight of honey, 0.0002% by weight of chitosanic acid amide, 0.0004% by weight of nicotinic acid amide, 0.0001% by weight of riboflavin sodium chloride, 0.0001% by weight of pyridoxine hydrochloride, 0.001% by weight of inositol, 0.002% by weight of orthoacetic acid, 98.7362% 1% by weight was added to prepare a health drink.

(3) health supplement manufacturing

A mixture of 55% by weight of spirulina, 10% by weight of guar gum enzyme hydrolyzate, 0.01% by weight of vitamin B1 hydrochloride, 0.01% by weight of vitamin B6 hydrochloride, 0.23% by weight of DL-methionine, 0.7% by weight of magnesium stearate, And 10% by weight of the radish extract were blended to prepare a refillable health supplement food according to a conventional method.

[Formulation Example 2: Preparation of a pharmaceutical composition for preventing or treating obesity]

In this Formulation Example, a pharmaceutical composition for preventing or treating obesity containing the 10% (v / v) fermented mugwort seed extract prepared in Example 1 was prepared as follows.

(1) Manufacture of powders

2 g of the radish extract was mixed with 1 g of lactose and filled in an airtight container to prepare a powder.

(2) Preparation of tablets

100 mg of the radish extract, 100 mg of the corn starch, 100 mg of the lactose and 2 mg of the magnesium stearate were mixed and tableted according to a conventional tablet preparation method.

(3) Manufacture of capsules

100 mg of radish extract, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate were mixed and filled in gelatin capsules to prepare capsules.

(4) Injection preparation

100 mg of the radish extract was dissolved in an appropriate amount of distilled water for injection and the pH was adjusted to about 7.5. Then, the solution was filled and sterilized in a 2 ml capacity ampoule to prepare an injection.

[Formulation Example 3: Preparation of animal drugs for prevention or treatment of obesity]

In the present formulation example, an animal drug for the prevention or treatment of obesity containing the 10% (v / v) fermented mugwort seed extract prepared in Example 1 was prepared as follows.

(1) Preparation of tablets

100 mg of the radish extract was mixed with 100 mg of the protein mixture and 100 mg of the endosperm, and the tablets were prepared by tableting according to a conventional preparation method.

(2) Injection preparation

100 mg of the radish extract was dissolved in an appropriate amount of distilled water for injection and the pH was adjusted to about 7.5. Then, the solution was filled and sterilized in a 2 ml capacity ampoule to prepare an injection.

[Formulation Example 4: Preparation of food composition for diabetic improvement]

In this Formulation Example, a diabetic food composition containing the 10% (v / v) fermented mugwort extract prepared in Example 1 was prepared as follows.

(1) biscuit manufacture

By weight of starch, 0.77% by weight of glucose, 0.78% by weight of glucose, 11.78% by weight of palm shortening, 1.54% by weight of ammonia, 0.17% by weight of sodium bicarbonate, 0.16% by weight of sodium bisulfite 1.45% by weight of rice flour, 0.0001% by weight of vitamin B1, 0.0001% by weight of vitamin B2, 0.04% by weight of milk fractions, 20.6998% by weight of water, 1.16% by weight of whole milk powder, 0.29% by weight of substitute milk powder, 0.29% by weight of salt and 7.27% by weight of spray oil and 1% by weight of the extract of Radix seed were mixed to prepare a biscuit by a conventional method.

(2) Health drinks manufacturing

0.2% by weight of honey, 0.0002% by weight of chitosanic acid amide, 0.0004% by weight of nicotinic acid amide, 0.0001% by weight of riboflavin sodium chloride, 0.0001% by weight of pyridoxine hydrochloride, 0.001% by weight of inositol, 0.002% by weight of orthoacetic acid, 98.7362% 1% by weight was added to prepare a health drink.

(3) health supplement manufacturing

A mixture of 55% by weight of spirulina, 10% by weight of guar gum enzyme hydrolyzate, 0.01% by weight of vitamin B1 hydrochloride, 0.01% by weight of vitamin B6 hydrochloride, 0.23% by weight of DL-methionine, 0.7% by weight of magnesium stearate, And 10% by weight of the radish extract were blended to prepare a refillable health supplement food according to a conventional method.

[Formulation Example 5: Preparation of pharmaceutical composition for prevention or treatment of diabetes]

In this Formulation Example, a pharmaceutical composition for preventing or treating diabetes containing 10% (v / v) fermented mugwort extract prepared in Example 1 was prepared as follows.

(1) Manufacture of powders

2 g of the radish extract was mixed with 1 g of lactose and filled in an airtight container to prepare a powder.

(2) Preparation of tablets

100 mg of the radish extract, 100 mg of the corn starch, 100 mg of the lactose and 2 mg of the magnesium stearate were mixed and tableted according to a conventional tablet preparation method.

(3) Manufacture of capsules

100 mg of radish extract, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate were mixed and filled in gelatin capsules to prepare capsules.

(4) Injection preparation

100 mg of the radish extract was dissolved in an appropriate amount of distilled water for injection and the pH was adjusted to about 7.5. Then, the solution was filled and sterilized in a 2 ml capacity ampoule to prepare an injection.

[Formulation Example 6: Production of animal medicines for prevention or treatment of diabetes]

In this formulation example, an animal medicine for the prevention or treatment of diabetes containing the 10% (v / v) fermented mugwort extract prepared in Example 1 was prepared as follows.

(1) Preparation of tablets

100 mg of the radish extract was mixed with 100 mg of the protein mixture and 100 mg of the endosperm, and the tablets were prepared by tableting according to a conventional preparation method.

(2) Injection preparation

100 mg of the radish extract was dissolved in an appropriate amount of distilled water for injection and the pH was adjusted to about 7.5. Then, the solution was filled and sterilized in a 2 ml capacity ampoule to prepare an injection.

[ Formulation Example  7: Preparation of food composition for fatty liver improvement]

In the present formulation example, a food composition for improving fatty liver containing the 10% (v / v) fermented fermented mugwort extract prepared in Example 1 was prepared as follows.

(1) biscuit manufacture

By weight of starch, 0.77% by weight of glucose, 0.78% by weight of glucose, 11.78% by weight of palm shortening, 1.54% by weight of ammonia, 0.17% by weight of sodium bicarbonate, 0.16% by weight of sodium bisulfite 1.45% by weight of rice flour, 0.0001% by weight of vitamin B1, 0.0001% by weight of vitamin B2, 0.04% by weight of milk fractions, 20.6998% by weight of water, 1.16% by weight of whole milk powder, 0.29% by weight of substitute milk powder, 0.29% by weight of salt and 7.27% by weight of spray oil and 1% by weight of the extract of Radix seed were mixed to prepare a biscuit by a conventional method.

(2) Health drinks manufacturing

0.2% by weight of honey, 0.0002% by weight of chitosanic acid amide, 0.0004% by weight of nicotinic acid amide, 0.0001% by weight of riboflavin sodium chloride, 0.0001% by weight of pyridoxine hydrochloride, 0.001% by weight of inositol, 0.002% by weight of orthoacetic acid, 98.7362% 1% by weight was added to prepare a health drink.

(3) health supplement manufacturing

A mixture of 55% by weight of spirulina, 10% by weight of guar gum enzyme hydrolyzate, 0.01% by weight of vitamin B1 hydrochloride, 0.01% by weight of vitamin B6 hydrochloride, 0.23% by weight of DL-methionine, 0.7% by weight of magnesium stearate, And 10% by weight of the radish extract were blended to prepare a refillable health supplement food according to a conventional method.

[Formulation Example 8: Preparation of a pharmaceutical composition for prevention or treatment of fatty liver]

In this Formulation Example, a pharmaceutical composition for prevention or treatment of fatty liver containing the radish extract prepared in Example 1 was prepared as follows.

(1) Manufacture of powders

2 g of the radish extract was mixed with 1 g of lactose and filled in an airtight container to prepare a powder.

(2) Preparation of tablets

100 mg of the radish extract, 100 mg of the corn starch, 100 mg of the lactose and 2 mg of the magnesium stearate were mixed and tableted according to a conventional tablet preparation method.

(3) Manufacture of capsules

100 mg of radish extract, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate were mixed and filled in gelatin capsules to prepare capsules.

(4) Injection preparation

100 mg of the radish extract was dissolved in an appropriate amount of distilled water for injection and the pH was adjusted to about 7.5. Then, the solution was filled and sterilized in a 2 ml capacity ampoule to prepare an injection.

[Formulation Example 9: Preparation of animal medicines for prevention or treatment of fatty liver]

In this formulation example, an animal medicine for prevention or treatment of fatty liver containing the 10% (v / v) fermented fermented mugwort extract prepared in Example 1 was prepared as follows.

(1) Preparation of tablets

100 mg of the radish extract was mixed with 100 mg of the protein mixture and 100 mg of the endosperm, and the tablets were prepared by tableting according to a conventional preparation method.

(2) Injection preparation

100 mg of the radish extract was dissolved in an appropriate amount of distilled water for injection and the pH was adjusted to about 7.5. Then, the solution was filled and sterilized in a 2 ml capacity ampoule to prepare an injection.

Claims (15)

Wherein the content of sulfolapin is increased such that the content of sulfolapin in the extract is in the range of 20.90 to 27.27 mg / g by adding 5 to 10% (v / v) ethanol as an extraction solvent to the sucrose. Way.
The method according to claim 1,
Preferably,
Wherein the extract is an immersion extract.
delete The method according to claim 1,
Preferably,
Wherein the reaction is carried out at a temperature of 35 to 45 캜 for 10 to 15 hours.
Extract of Sucroflavin, which is obtained by adding 5 ~ 10% (v / v) ethanol as an extraction solvent to sucrose and containing sulfolapin in the extract at 20.90 ~ 27.27 mg / g.
delete The extracts were prepared by adding 5 ~ 10% (v / v) ethanol as an extraction solvent to sucrose. The extracts containing 20 ~ 27.27 mg / g sulfolapin in the extract Food composition.
(Ii) an anti-obesity drug which contains an extract of Ricinus with an increased sulfolane content so that sulfolapin is contained in the extract at a concentration of from 20.90 to 27.27 mg / g, obtained by adding 5-10% (v / v) A pharmaceutical composition for therapeutic use.
(Ii) an anti-obesity drug which contains an extract of Ricinus with an increased sulfolane content so that sulfolapin is contained in the extract at a concentration of from 20.90 to 27.27 mg / g, obtained by adding 5-10% (v / v) A therapeutic veterinary pharmaceutical composition.
It was extracted by adding 5 ~ 10% (v / v) ethanol as an extraction solvent to mucilage. It was used for the improvement of diabetes mellitus, which contained an extract of mucilaginous sulforaphin, which contained sulfolapin in the extract at 20.90 ~ 27.27 mg / Food composition.
(V / v) ethanol as an extractive solvent to prevent the diabetes mellitus containing scorpion extract-rich radish extract so that sulfolapin is contained in the extract at 20.90 to 27.27 mg / g. A pharmaceutical composition for therapeutic use.
(V / v) ethanol as an extractive solvent to prevent the diabetes mellitus containing scorpion extract-rich radish extract so that sulfolapin is contained in the extract at 20.90 to 27.27 mg / g. A therapeutic veterinary pharmaceutical composition.
It was extracted by adding 5 ~ 10% (v / v) ethanol as an extraction solvent to sucrose, and it was found that the sulfolafin contained in the extract was contained at 20.90 ~ 27.27 mg / g. Food composition.
(V / v) ethanol as an extractive solvent, and it has been found that the prevention and / or prevention of fatty liver disease, which comprises the extract of the radish with increased sulfolane content so that sulfolapin is contained in the extract at 20.90 to 27.27 mg / A pharmaceutical composition for therapeutic use.
(V / v) ethanol as an extractive solvent, and it has been found that the prevention and / or prevention of fatty liver disease, which comprises the extract of the radish with increased sulfolane content so that sulfolapin is contained in the extract at 20.90 to 27.27 mg / A therapeutic veterinary pharmaceutical composition.

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