KR100664738B1 - Herb water which has exellent flavor and anti-cancer activity - Google Patents

Abstract

Provided are a method for preparing a herb water, and a herb water prepared by the method which is excellent in flavor, has an antitumor effect, reduces weight and promotes evacuation. The method comprises the steps of vacuum freeze-drying herb at a temperature of -70 to -90 deg.C, extracting the dried one at a temperature of 40-50 deg.C for 1-3 hours, and maturing the extracted one at a low temperature of 2-10 deg.C for 1 hour to 3 days to prepare a herb extract; mixing a herb sprout and a herb flower with rock water, grinding the mixture with a mixer and maturing the mixture at a low temperature of 2-10 deg.C for 1 hour to 3 days to prepare a juice of a herb sprout and a herb flower; mixing 100 parts by weight of rock water, 1-30 parts by weight of the herb extract and 1-30 parts by weight of the juice; and filtering and sterilizing the obtained mixture.

Description

Herbal water which has exellent flavor and anti-cancer activity

The present invention relates to an anticancer functional herbal water excellent in sensory beauty, and more particularly, an extract obtained by low temperature aging after extracting a herbaceous plant, and sprouts and herb flowers mixed with rock water, followed by a blender, followed by low temperature aging. The present invention relates to a herb water which is mixed with liquid, filled with rock water, filtered and sterilized, and has excellent functional beauty and anticancer function.

Water makes up about 70% of our body and is one of the most important components of our body. Therefore, it is important to consume a certain amount of water every day for physical health. Especially, due to concern about environmental pollution and well-being fever spreading in society, the interest in 'health' and 'beauty' is very important. With the heightened relationship, the importance of 'water', which is the most basic element of life, is more important than ever.

On the other hand, herbs are herbaceous plants in which flowers, seeds, stems, leaves, and roots are useful to humans, and have played an important role as herbs for the treatment of antiseptics and soothes, as well as antiseptics and insecticides. It is a natural material that has attracted much more attention due to its toxicity to the human body, concern about environmental pollution and its excellent efficacy.

Herbs typically have three benefits: First, they provide the effects of relaxation and relaxation due to the inherent aroma of herbs. Second, the herbs contain a large amount of minerals to naturally regulate the condition of the body. To increase resistance to disease, and third, the herb has a bitter taste to promote the secretion of gastric juice, improve appetite and improve digestion.

ROSEMARY is a perennial evergreen plant with Lamiaceae, usually growing more than 1 meter. Narrow, narrow, needle-like leaves have a tough, leathery, glossy, distinctive strong aroma, dark green, disinfectant, used for preserving meat, and used in hospitals and epidemics. Rosemary helps to improve memory, helps rejuvenate when you are feeling helpless, and eliminates headaches and migraines. In addition, it has an anti-obesity effect, rejuvenates the skin to revitalize sagging tissues, suppresses the occurrence of edema and dandruff, and promotes hair growth. Rosemary is used for cooking, facial cleansing, and to increase blood pressure during low blood pressure.

Lavender is the most known variety of herbs. The fragrance is popular as an ornamental herb because it appears to shine on the surface of leaves and flowers. High-quality lavender is used for many purposes, and it is especially suitable for potpourri and various beauty products as it has a fragrance all over plants such as flowers, leaves and stems. In the morning, dry flowers squeeze purple buds before flowering and hang them in a well-ventilated shade. Ornamental leaves are cut off after leaving two flowers. The lotion made with the essential oils of lavender has a cleansing effect that relaxes, relaxes and refreshes the skin. Lavender scent also has a mental calming effect, which is used for sleeping on a pillow. Boiling tea with lavender can be effective for sedation, relieves pain and headaches, and can help you sleep better.

Mint (MINT) is native to Europe, and lemon balm, together with the representative herbs of wheat plants. It is one of the strongest herbs growing in perennial herbaceous plants, and has a strong peppermint scent that feels refreshing and refreshing just by touching the leaves. When storing, cut the root of the stem in June and July, when the fragrance is strongest, and dry it in the shade. Mint herb, which is characterized by its refreshing sensation, is especially used for making sauces for meat dishes, and is used for flavors such as beanies, sparkling water drinks, and cocktails, which are used in vegetable or fruit salads with fresh taste and aroma. Mints are somewhat different depending on the type, but they are used as raw materials for sterilization, digestion, health, oral deodorant, toothpaste and placebo. It is effective for dermatitis and itching, and when placed in the bathroom, peppermint scent lasts for a long time instead of odor, and a strong sterilization effect. Green peppermint, called spearmint, is often used in cooking with peppermint, which is sweeter than peppermint and is very good for the skin.

CHAMOMILE is a herb among many herbs and is one of the best known herbs since ancient times. It is native to Europe, India, and North Africa, and belongs to the Asteraceae. The fragrance has a sweet and sour apple flavor. In spring, small flowers bloom from place to place, spreading the sweet fragrance. Dry flowers are used as a bath because of their beauty. Adding a small amount to your massage oil will help you recover from fatigue after exercise. In Europe, it is reminiscent of chamomile, but the generic name "Matricaria" is due to the effect on the womb. Hypoallergenic, antiseptic, antiparasitic medicines, good for calming cramps. In addition, there is an effect of keeping the body warm for people with coldness. A few days after flowering, the fragrance of flowers is good and tastes good. Incense relieves anxiety, anger or severe mental strain. Add 5 or 6 small white flowers to hot water to enjoy a sweet apple-flavored tea.

SAGE has been used as a panacea since Greek and Roman times. It is used in various ways such as ornamental, flavoring, medicine, cooking, dyeing, tonic, soothing, digestion, sterilization effect, and neutralizes the fat of meat or fish to remove odors. Sage has a variety of uses, but should not be used in large quantities at one time. Sage tea clears the mood, calms the excitement, and is effective in preventing mouth bleeding and bleeding and bad breath. Sage is also used to promote digestion, detoxification, placebo, and bathing agents as a remedy for fatigue, expectoration, gout, diuresis, insect repellent, and antiseptic action.

Oregano is a perennial with a leaflet and its origin is Europe. It is well used in Italian and Mexican cuisine and has a wild scent as the wild marjoram. Goes well with Latin dishes and tomatoes. It promotes digestion and tonic, and is used as tea, bath or dry flower.

The thyme scientific name means `` scenting '' small flowers of various colors ranging from early summer to white to pink. It is an evergreen small tree with Lamiaceae. It has a strong aroma of mint not only in the stem but also throughout the grass. It is effective in combating fleas, insects in the house and insects such as mosquitoes and flies. It is also suitable for indoor potted plants and has a scent. Used for cooking, potpourri, baths, and dyes, a mixture of hot water and a few drops of thyme herbal oil inhale water vapor to prevent colds and soften sore throats. The leaves have a mental tonic effect and are effective against depression. It also has a strong bactericidal action and can be used as an indoor fragrance.

Recently, it is known that nutrient tonic components such as various nutrients and growth-related enzymes necessary for growth in the shoots of plants are aggregated. No. 2002-33298), such as a variety of products using a sprout extract has been disclosed, it is known that the amount of active ingredients such as vitamins and minerals in the shoots of about 3 to 20 times more than a fully grown plant. Particularly, in the case of vitamin B, the content increases 3 to 15 times between 3 to 6 days after germination, and 5 to 10 days after germination has been reported to have the highest nutritional density. In addition, new shoots are known to grow hair, prevent aging and diet, and broccoli sprouts have been found to contain high amounts of powerful antioxidants that prevent cancer.

The present invention has been made in view of the above, the extract obtained by low-temperature aging of the leaves, stems or flowers of the mature herb plants after low-temperature aging, mixed with herb sprout sprouts and herb flowers in rock water and then aged at low temperature The purpose of the present invention is to provide drinking water substitutes equipped with weight loss, bowel movement promoting function and anti-cancer function as well as functional beauty obtained by mixing and filtering the sterilized juice.

In order to achieve the above object, in the present invention, the extract, obtained by low temperature aging of leaves, stems, or flower parts of mature herb plants, is mixed with low-temperature aging, and mixed with a fresh juice made by mixing low-temperature aging after mixing herb sprout sprouts and herb flowers with rock water. By filtration and sterilization treatment, it was achieved by providing herbal water, which is a water substitute drink equipped with not only sensuality but also weight loss, bowel movement promoting function and anticancer function.

Hereinafter, the configuration of the present invention.

In accordance with the above object, the present invention provides a herb extract, a herb water, which is a substitute for water containing the fresh juice of the herb sprout and herb flowers.

Herb water production method of the present invention is characterized by consisting of the following steps.

Step 1: prepare herbal extracts

The selected herbs are vacuum-freeze-dried at -70 ° C to -90 ° C, and the dried raw material is extracted at low temperature for 40 hours at 40-50 ° C for 1-3 hours, and then has a low temperature aging time for 1 hour to 3 days at 2-10 ° C.

Second step: preparing the juice of sprout sprouts and herb flowers

Sprout sprout and herbal flowers are mixed with natural rock water to grind with a blender to prepare a juice solution, the juice solution is aged at low temperature for 2 hours to 10 ℃ for 1 hour to 3 days.

Step 3: mix the extract with fresh juice

100 parts by weight of natural rock water, 1-30 parts by weight of the herbal extract of the first step and 1-30 parts by weight of the fresh juice of the second step are mixed.

Step 4: Filtration and Sterilization

The solution obtained in the third step is filtered to remove solids and sterilized by ultraviolet rays.

In the herb water producing process of the present invention, bud bud and herb flowers may be mixed in an arbitrary ratio, but preferably bud bud: herb flowers are mixed in a ratio of 10: 0.1 to 5. In the above ratio, the sensory taste of the present invention is better because the herb water is too much, and if the herbal order is too much, the taste is bitter and hard, and if the herbal order is too small, a lot of herbal flowers are required, but the production of herb flowers is difficult.

In addition, it is more preferable to stir the mixed solution at 500-1500rpm for 1-3 hours when mixing the herbal extract and the juice.

Herb plants in the present invention can be used any one or more selected from the group consisting of rosemary, lavender, mint, chamomile, sage, oregano and thyme.

Hereinafter, a specific method of the present invention will be described in detail with reference to Examples, and the scope of the present invention is not limited only to these Examples.

Example 1 Rosemary Herb Water Preparation

Step 1: Preparation of Herb Plant Extracts

The rosemary leaves were cut and washed thoroughly and then vacuum lyophilized at -80 ° C. The dried rosemary leaves were put in water and extracted at 45 ° C. for 2 hours at low temperature. The extract was put into a low-temperature storage cell at low temperature for 4 days at 4 ℃ to prepare an extract of the herbal plant.

Second step: preparing fresh juice of sprout and herb flowers

After cutting and washing rosemary sprout and rosemary flowers grown to 1.5cm, 1kg of the mixture obtained by mixing at a ratio of 10: 1 is mixed with 10L of natural rock water, and then mixed with a blender to prepare a fresh juice solution. After aging at low temperature to prepare a juice.

Step 3: Mix the Extract with the Juice

5 parts by weight of the mixture obtained by mixing the same amount of the extract and the juice prepared in the first and second steps to 100 parts by weight of natural rock, respectively, was stirred at 1000rpm for 2 hours.

Step 4: Filtration and Sterilization

The rosemary herb water of the present invention was prepared by removing solids by filtering the solution prepared in the third step using an ultra-precision filtration device, preparing a clear solution, and then sterilizing using a UV-flowing sterilizer.

Example 2: Lavender Herb Water Preparation

Lavender herb water was prepared in the same manner as in Example 1 using lavender instead of rosemary as an herbaceous plant.

Example 3: Mint Herb Water Preparation

Mint herb water of the present invention was prepared in the same manner as in Example 1 using mint instead of rosemary as a herbaceous plant.

Example 4: Chamomile Herb Water Preparation

Chamomile herb water of the present invention was prepared in the same manner as in Example 1 using chamomile instead of rosemary as a herbaceous plant.

Example 5: Sage Herb Water Preparation

Sage herb water of the present invention was prepared in the same manner as in Example 1 using sage instead of rosemary as a herbaceous plant.

Example 6: Preparation of Oregano Herb Water

Oregano herbal water of the present invention was prepared in the same manner as in Example 1 using oregano instead of rosemary as a herbaceous plant.

Example 7: Time Herb Water Preparation

The thyme herb water of the present invention was prepared in the same manner as in Example 1 using thyme instead of rosemary as a herbaceous plant.

Example 8 Preparation of Mixed Herbal Water

The herbal mixture of the present invention was prepared in the same manner as in Example 1 using a mixture of rosemary, lavender, mint, chamomile, sage, oregano, and thyme as a herbaceous plant, in the same manner.

Experimental Example 1 Investigation of Cancer Cell Growth Inhibition Effect

The cancer cell growth inhibitory effect of the herbal water prepared in Examples 1 to 8 was investigated as follows.

After culturing the mouse pancreatic MS 1 cell line (ATCC, USA) and human blood cancer cells HL60 cell line (ATCC, USA), 0.5% dimethylsulfoxide (DMSO) was used as a negative control and a positive control. Camptothecin (final concentration 0.5 μg / ml) was treated and subject test substances were treated by concentration. After 48 hours of incubation, the solution was dissolved in lysis buffer, and the reaction mixture containing MTT was added thereto and reacted in an incubator at 37 ° C. for 50 minutes, and then the absorbance was measured at 610 nm using a microplate reader ( Scott, G, J. Clin.Microbial . , 36, pp 362-366, 1998).

The experimental results are shown in Table 1 below. As can be seen in Table 1, the herbal waters of the present invention showed a cell growth inhibition rate of 50% or more with respect to MS 1, which is a mouse pancreatic cell line, at 30 to 60 µg / ml, and human blood cancer cells at 10 to 40 µg / ml. 50% cell growth inhibition rate was shown for the HL60 cell line, it can be seen that the herb number of the present invention has an excellent cancer cell growth inhibitory effect.

The concentration of the herb water of the present invention shows a cell growth inhibition rate of 50% or more for each cell line division MS 1 cell line HL60 cell line Control (Camtotechin) 0.36 0.32 Example 1 56.8 39.1 Example 2 49.2 32.3 Example 3 35.9 21.3 Example 4 59.3 39.9 Example 5 48.3 32.5 Example 6 42.6 28.6 Example 7 35.6 18.3 Example 8 30.0 10.0

Experimental Example 2: Investigation of weight loss effect and bowel movement promoting effect

Among five male and female volunteers with constipation and obesity, five randomly selected experimenters of the same height (male: 158cm, female: 145cm, female: 175cm, female: 30cm: 160cm) , After drinking the herb water prepared in Examples 1 to 8 three times a day for breakfast, lunch, and dinner for 200cc each month, the weight change and the degree of constipation improvement were measured and the results are shown in Tables 2 to 9 below. Shown in At this time, no drugs other than the herbal water were taken.

Weight loss and bowel movement promoting effect of rosemary herb water of the present invention division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 75.6 4 days 71.7 1 day female 66.3 4 days 60.5 1 day 30 spaces south 100.3 4 days 93.1 1 day female 82.3 4 days 75.9 1 day NOTE Each value is the average of five values.

Weight loss and bowel movement promoting effect of the lavender herb water of the present invention division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 76.1 4 days 71.0 1 day female 65.6 4 days 60.1 1 day 30 spaces south 101.5 4 days 94.6 1 day female 83.3 4 days 74.5 1 day NOTE Each value is the average of five values.

Weight loss effect and bowel movement promoting effect of the mint herbal water of the present invention division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 73.9 4 days 69.3 1 day female 67.5 4 days 61.7 1 day 30 spaces south 105.2 4 days 97.0 1 day female 82.0 4 days 75.4 1 day NOTE Each value is the average of five values.

Weight loss and bowel movement promoting effect of chamomile herb water of the present invention division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 73.7 4 days 68.3 1 day female 64.8 4 days 59.2 1 day 30 spaces south 102.4 4 days 94.5 1 day female 83.1 4 days 76.8 1 day NOTE Each value is the average of five values.

Weight loss effect and bowel movement promoting effect of the sage herb water of the present invention division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 74.8 4 days 69.1 1 day female 66.5 4 days 60.2 1 day 30 spaces south 104.8 4 days 95.7 1 day female 82.1 4 days 74.8 1 day NOTE Each value is the average of five values.

Weight loss and bowel movement promoting effect of the oregano herbal water of the present invention division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 77.4 4 days 72.8 1 day female 65.7 4 days 59.6 1 day 30 spaces south 110.4 4 days 100.8 1 day female 84.5 4 days 76.2 1 day NOTE Each value is the average of five values.

Weight loss effect and bowel movement promoting effect of the time herbal water of the present invention division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 74.8 4 days 72.1 1 day female 65.3 4 days 61.5 1 day 30 spaces south 107.1 4 days 98.4 1 day female 83.5 4 days 74.7 1 day NOTE Each value is the average of five values.

Weight loss effect and bowel movement promoting effect of the mixed herbal water of the present invention prepared in Example 8 division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 77.2 4 days 71.1 1 day female 68.1 4 days 58.7 1 day 30 spaces south 103.5 4 days 90.2 1 day female 84.7 4 days 74.3 1 day NOTE Each value is the average of five values.

As can be seen from Table 2 to Table 9, the herb water of the present invention was confirmed to have an excellent constipation improvement effect and excellent weight loss effect of 100%, it was found that the effect is particularly excellent in mixed herb water. . All the experimenters appreciated that the faster the recovery of fatigue and faster digestion when ingesting the herbal water of the present invention than when the general water.

Experimental Example 3: sensory test

The sensory test on the number of herbs prepared in Examples 1 to 8 was carried out as follows. The results are shown in Table 10 below.

The sensory test was performed by randomly extracting 10 men and women aged 20 to 50 years to obtain a nine-point grading system (9 = best; 8 = very good; 7 = usually good) for taste, taste, color, and texture. Good; 6 = Slightly good; 5 = Not good or hate; 4 = Slightly disliked; 3 = Normal dislikes; 2 = Very disliked; 1 = Most disliked; The average value was calculated | required. In addition, commercially available bottled water and barley water were purchased for comparison.

Sensory test result of hub water of the present invention division Commercial Bottled Water Commercial lime tree Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 incense 4.3 4.7 8.5 8.7 8.6 8.4 8.3 8.7 8.6 8.8 flavor 3.7 5.2 8.6 8.7 8.6 8.3 8.4 8.6 8.3 8.7 color 5.1 6.2 8.9 8.9 8.9 8.8 8.8 8.8 8.7 8.9 touch 5.0 4.6 8.4 8.5 8.3 8.3 8.4 8.5 8.4 8.6 Overall preference 4.5 5.2 8.6 8.7 8.6 8.5 8.5 8.7 8.5 8.8

As can be seen in Table 10, the herbal water of the present invention was found to be much preferred by consumers in terms of sensuality compared to commercially available bottled or barley water. Therefore, the herbal water of the present invention can be usefully used as a beverage for water and was expected to have excellent purchase symbol in the market.

As described above, the present invention, as described in the above embodiment, the extract obtained by low temperature aging of the herbaceous plant and low temperature aging, the shoot sprouts and herb flowers are mixed with rock water, mixed with a blender and then aged at low temperature to mix the juice to rock water. By filling and filtering and sterilizing, it is very useful and groundbreaking invention in the food industry because it can provide a herb water with excellent aroma and taste, excellent weight loss and bowel movement promoting function as well as anticancer function.

Claims (3)

The herb plant was vacuum-freeze-dried at -70 ° C to -90 ° C to extract dried raw material at 40-50 ° C for 1-3 hours, and then aged at low temperature at 2 to 10 ° C for 1 hour to 3 days to prepare an herbal extract. step; Mixing sprout sprouts and herbal flowers with natural rock water, going to a blender, and then aging at 2-10 ° C. for 1 hour to 3 days to produce a fresh juice of sprout sprouts and herbal flowers; Mixing 100 parts by weight of natural rock water, 1-30 parts by weight of the herbal extract of the first step and 1-30 parts by weight of the fresh juice of the second step; And Method for producing a herbal water, characterized in that consisting of the step of filtering and sterilizing the mixed solution obtained in the step. The method of claim 1, wherein the herb plant is any one or more selected from the group consisting of rosemary, lavender, mint, chamomile, sage, oregano and thyme. A herb water, characterized by excellent functionality and anticancer functionality produced by the method according to claim 1 or 2.