KR100642868B1 - Herb soybean paste which has exellent flavor and anti-cancer activity - Google Patents

Abstract

Provided is functional fermented soybean paste(toenjang) containing an herb extract, which has excellent organoleptic property and anticancer property. The titled fermented soybean paste is prepared by mixing 100 parts by weight of fermented soybean paste and 0.1 to 10 parts by weight of raw juice and then fermenting. The herb extract is obtained by freeze drying an herb plant at -70 to -90deg.C under vacuum, extracting at 40 to 50deg.C for 1 to 3hr and aging at 2 to 10deg.C for 1 to 3 days. The raw juice is obtained by adding natural underground water to sprouts and herb flowers, grinding with a grinding mixer and aging at 2 to 20deg.C for 1 to 3 days. The herb plant is any one selected from the group consisting of rosemary, lavender, mint, chamomile, clary sage, oregano, thyme, lemon grass, rose hip, echinacea, jasmine, sweet fennel, mate, ginger, eucalyptus, stevia, jasmine, lilac, orange flower, eyebright, orchid and mauve.

Description

Herbal soybean paste with excellent sensuality {Herb soybean paste which has exellent flavor and anti-cancer activity}

The present invention relates to an anti-cancer functional herb soybean with excellent sensuality. More specifically, the extract obtained by low temperature aging of herbal plants and low temperature aging, mixed with shoot sprouts and herb flowers in rock water, mixed with a blender, The present invention relates to a herb miso prepared by using a mixed liquid obtained by mixing the liquid with excellent functional beauty and anticancer function.

Doenjang is jang soaked in meju and scooped out the rest of the soup. Doenjang is a Korean seasoned food that has been handed down with soy sauce for a long time. It is the basis for seasoning and flavoring foods. Soybean paste made with soybeans containing 38% protein and 18% fat containing a lot of unsaturated fatty acids such as linoleic acid and linolenic acid has 128 calories per 100g, protein 12g, 4.1g fat, 14.5g carbohydrates and 17.9g ash. This is a rich food. Doenjang is also used as a medicine, and in Dong-Bo's agreement, it is said to cure the headache and heat.

On the other hand, herbs are herbaceous plants in which flowers, seeds, stems, leaves, and roots are useful to humans, and have played an important role as herbs for the treatment of antiseptics and soothes, as well as for preservation and insecticide. It is a natural material that is receiving much more attention due to its toxicity to chemicals, concern about environmental pollution and its excellent efficacy.

Herbs typically have three benefits: First, they provide the effects of relaxation and relaxation due to the inherent aroma of herbs. Second, the herbs contain a large amount of minerals to naturally regulate the condition of the body. To increase resistance to disease, and third, the herb has a bitter taste to promote the secretion of gastric juice, improve appetite and improve digestion.

ROSEMARY is a perennial evergreen plant with Lamiaceae, usually growing more than 1 meter. Narrow, narrow, needle-like leaves have a tough, leathery, glossy, distinctive strong aroma, dark green, disinfectant, used for preserving meat, and used in hospitals and epidemics. Rosemary helps to improve memory, helps rejuvenate when you are feeling helpless, and eliminates headaches and migraines. In addition, it has an anti-obesity effect, rejuvenates the skin to revitalize sagging tissues, suppresses the occurrence of edema and dandruff, and promotes hair growth. Rosemary is used for cooking, facial cleansing, and to increase blood pressure during low blood pressure.

Lavender is the most known variety of herbs. The fragrance is popular as an ornamental herb because it appears to shine on the surface of leaves and flowers. High-quality lavender is used for many purposes, and it is especially suitable for potpourri and various beauty products as it has a fragrance all over plants such as flowers, leaves and stems. In the morning, dry flowers squeeze purple buds before flowering and hang them in a well-ventilated shade. Ornamental leaves are cut off after leaving two flowers. The lotion made with the essential oils of lavender has a cleansing effect that relaxes, relaxes and refreshes the skin. Lavender scent also has a mental calming effect, which is used for sleeping on a pillow. Boiling tea with lavender can be effective for sedation, relieves pain and headaches, and can help you sleep better.

Mint (MINT) is native to Europe, and lemon balm, together with the representative herbs of wheat plants. It is one of the strongest herbs growing in perennial herbaceous plants, and has a strong peppermint scent that feels refreshing and refreshing just by touching the leaves. When storing, cut the root of the stem in June and July, when the fragrance is strongest, and dry it in the shade. Mint herb, which is characterized by its refreshing sensation, is especially used for making sauces for meat dishes, and is used for flavors such as beanies, sparkling water drinks, and cocktails, which are used in vegetable or fruit salads with fresh taste and aroma. Mints are somewhat different depending on the type, but they are used as raw materials for sterilization, digestion, health, oral deodorant, toothpaste and placebo. It is effective for dermatitis and itching, and when placed in the bathroom, peppermint scent lasts for a long time instead of odor, and a strong sterilization effect. Green peppermint, called spearmint, is often used in cooking with peppermint, which is sweeter than peppermint and is very good for the skin.

CHAMOMILE is a herb among many herbs and is one of the best known herbs since ancient times. It is native to Europe, India, and North Africa, and belongs to the Asteraceae. The fragrance has a sweet and sour apple flavor. In spring, small flowers bloom from place to place, spreading the sweet fragrance. Dry flowers are used as a bath because of their beauty. Adding a small amount to your massage oil will help you recover from fatigue after exercise. In Europe, it is reminiscent of chamomile, but the generic name "Matricaria" is due to the effect on the womb. Hypoallergenic, antiseptic, antiparasitic medicines, good for calming cramps. In addition, there is an effect of keeping the body warm for people with coldness. A few days after flowering, the fragrance of flowers is good and tastes good. Incense relieves anxiety, anger or severe mental strain. Add 5 or 6 small white flowers to hot water to enjoy a sweet apple-flavored tea.

SAGE has been used as a panacea since Greek and Roman times. It is used in various ways such as ornamental, flavoring, medicine, cooking, dyeing, tonic, soothing, digestion, sterilization effect, and neutralizes the fat of meat or fish to remove odors. Sage has a variety of uses, but should not be used in large quantities at one time. Sage tea clears the mood, calms the excitement, and is effective in preventing mouth bleeding and bleeding and bad breath. Sage is also used to promote digestion, detoxification, placebo, and bathing agents as a remedy for fatigue, expectoration, gout, diuresis, insect repellent, and antiseptic action.

Oregano is a perennial with a leaflet and its origin is Europe. It is well used in Italian and Mexican cuisine and has a wild scent as the wild marjoram. Goes well with Latin dishes and tomatoes. It promotes digestion and tonic, and is used as a tea, bath or dry flower.

The thyme scientific name means `` scenting '' small flowers of various colors ranging from early summer to white to pink. It is an evergreen small tree with Lamiaceae. It has a strong aroma of mint not only in the stem but also throughout the grass. It is effective in combating fleas, insects in the house and insects such as mosquitoes and flies. It is also suitable for indoor potted plants and has a scent. Used for cooking, potpourri, baths, and dyes, a mixture of hot water and a few drops of thyme herbal oil inhale water vapor to prevent colds and soften sore throats. The leaves have a mental tonic effect and are effective against depression. It also has a strong bactericidal action and can be used as an indoor fragrance.

Recently, it is known that nutrient tonic components such as various nutrients and growth-related enzymes necessary for growth in the shoots of plants are aggregated. No. 2002-33298), such as a variety of products using a sprout extract has been disclosed, it is known that the amount of active ingredients such as vitamins and minerals in the shoots of about 3 to 20 times more than a fully grown plant. Particularly, in the case of vitamin B, the content increases 3 to 15 times between 3 to 6 days after germination, and 5 to 10 days after germination has been reported to have the highest nutritional density. In addition, new shoots are known to grow hair well, prevent aging and diet, and broccoli shoots have been found to contain high amounts of powerful antioxidants that prevent cancer.

The present invention was conceived in view of the above points, the extract obtained by low-temperature aging after extracting the herb plants, and the mixture of shoot sprouts and herb flowers in rock water to go to a blender and then mixed with a fresh juice obtained by low-temperature aging The purpose of the present invention is to provide a herb miso prepared with excellent functional beauty and anti-cancer function prepared using the obtained mixed liquid.

In order to achieve the above object, the present invention uses a mixture obtained by mixing the extract obtained by low temperature aging of the herbal plants and then low temperature aging, the sprout sprouts and herb flowers mixed with rock water, followed by a blender and the raw juice obtained by low temperature aging. It was achieved by providing a herbal miso prepared with excellent functional beauty and anti-cancer function.

Hereinafter, the configuration of the present invention.

In accordance with the above object, the present invention provides a herb miso obtained by mixing the herb extract and the juice of the herb shoots and herbal flowers and fermented by mixing with other ingredients in the preparation of miso.

Herbal miso manufacturing method of the present invention is characterized by consisting of the following steps.

Step 1: prepare herbal extracts

The selected herbs are vacuum-freeze-dried at -70 ° C to -90 ° C, and the dried raw material is extracted at low temperature for 40 hours at 40-50 ° C for 1-3 hours, and then has a low temperature aging time for 1 hour to 3 days at 2-10 ° C.

Second step: preparing the juice of sprout sprouts and herb flowers

Sprout sprout and herbal flowers are mixed with natural rock water to grind with a blender to prepare a juice solution, the juice solution is aged at low temperature for 2 hours to 10 ℃ for 1 hour to 3 days.

Step 3: mix the extract with fresh juice

0.1-10 parts by weight of the juice solution of the second step is mixed with 1 part by weight of the herbal extract of the first step.

Step 4: make miso

To prepare the doenjang by the general doenjang manufacturing method, but mixed with 0.1-10 parts by weight of the mixed solution of the herbal extract and fresh juice of the above step based on 100 parts by weight of the total weight and then fermented to produce the herbal doenjang of the present invention.

In the herb miso manufacturing process of the present invention, bud sprout and herb flowers may be mixed at an arbitrary ratio, but preferably, bud shoot: herb flowers are mixed at a ratio of 10: 0.1 to 5. The above-mentioned ratio is because the sensory beauty of the present invention of the herb miso is better, and if the herbal order is too much, the taste is bitter and bitter, and if the herbal order is too small, a lot of herbal flowers are required, but the production of herbal flowers is difficult.

In addition, it is more preferable to stir the mixed solution at 500-1500rpm for 1-3 hours when mixing the herbal extract and the juice.

In the preparation of the herb miso of the present invention, any method for preparing miso may be used as long as it is a general miso manufacturing method known in the art. However, a feature of the present invention is that fermentation is performed by putting together a mixed solution of herbal extracts and fresh juices when mixing materials such as meju.

Preferred process of the doenjang manufacturing step of the method of producing a herb miso is as follows:

Mixing 30-50 parts by weight of meju powder, 30-50 parts by weight of water and 10-30 parts by weight of salt;

Adding and mixing 0.1-10 parts by weight of the mixed solution of the herbal extract and the juice solution to the mixture of the step; And

Fermenting the mixture at room temperature for 1-3 months.

Herb plants in the present invention, rosemary, lavender, mint, chamomile, sage, oregano, thyme, lemongrass, rosehip, echinacea, jacques, sweet fennel, mate, yukari, ginger, chamomile, stevia, jasmine, lilac, orange Any one or more selected from the group consisting of flower, eye bright, orchid, and mauve may be used, but the present invention is not limited thereto, and may be arbitrarily selected from edible herb plants, and two or more kinds of herbal extracts may be arbitrarily combined. .

Hereinafter, a specific method of the present invention will be described in detail with reference to Examples, and the scope of the present invention is not limited only to these Examples.

Example 1 rosemary herb miso

Step 1: Preparation of Herb Plant Extracts

The rosemary leaves were cut and washed thoroughly and then vacuum lyophilized at -80 ° C. The dried rosemary leaves were put in water and extracted at 45 ° C. for 2 hours at low temperature. The extract was put into a low-temperature storage cell at low temperature for 4 days at 4 ℃ to prepare an extract of the herbal plant.

Second step: preparing fresh juice of sprout and herb flowers

After cutting and washing rosemary sprout and rosemary flowers grown to 1.5cm, 1kg of the mixture obtained by mixing at a ratio of 10: 1 is mixed with 10L of natural rock water, and then mixed with a blender to prepare a fresh juice solution. After aging at low temperature to prepare a juice.

Step 3: Mix the Extract with the Juice

An equal amount of the extract and the juice prepared in each of the first and second stages were mixed and stirred at 1000 rpm for 2 hours.

Step 4: make miso

Meju powder 8kg, water 8kg, salt 2.5kg were mixed, and 150g of the mixed solution of the herbal extract prepared in the above step and the juice was added thereto, followed by fermentation at room temperature for 2 months to prepare the rosemary herb miso of the present invention.

Example 2: Lavender Herb Miso

Lavender herb miso was prepared in the same manner as in Example 1 using lavender instead of rosemary as an herbal plant.

Example 3: Mint Herb Miso Preparation

Mint herb miso was prepared in the same manner as in Example 1 using mint instead of rosemary as a herbaceous plant.

Example 4: Chamomile Herb Miso Preparation

Chamomile herb miso was prepared in the same manner as in Example 1 using chamomile instead of rosemary as a herbaceous plant.

Example 5: Sage Herb Miso Preparation

Sage herb miso was prepared in the same manner as in Example 1 using sage instead of rosemary as a herbaceous plant.

Example 6 Preparation of Oregano Herb Miso

Oregano herb miso was prepared in the same manner as in Example 1 using oregano instead of rosemary as a herbaceous plant.

Example 7: Time Herb Miso Preparation

Using the thyme instead of rosemary as a herbaceous plant was prepared in the present invention the thyme herb in the same manner as in Example 1.

Example 8: Mixed Herbal Miso Preparation

The herbal mixture of the present invention was prepared in the same manner as in Example 1 using a mixture of rosemary, lavender, mint, chamomile, sage, oregano, and thyme as a herbaceous plant, in the same manner.

Comparative Example 1: General Miso Preparation

Miso was prepared in the same manner as in Example 1, except that the herbal extract and the herbal sap and the fresh juice of the herb flowers were mixed and mixed during preparation of the miso.

Experimental Example 1 Investigation of Cancer Cell Growth Inhibition Effect

Herbal doenjang prepared in Examples 1 to 8 and the cancer cell growth inhibitory effect of the doenjang prepared in Comparative Example 1 was investigated by comparing as follows.

After culturing the mouse pancreatic MS 1 cell line (ATCC, USA) and human blood cancer cells HL60 cell line (ATCC, USA), 0.5% dimethylsulfoxide (DMSO) was used as a negative control and a positive control. Camptothecin (final concentration 0.5 μg / ml) was treated and subject test substances were treated by concentration. After 48 hours of incubation, the solution was dissolved in lysis buffer, and the reaction mixture containing MTT was added thereto and reacted in an incubator at 37 ° C. for 50 minutes, and then the absorbance was measured at 610 nm using a microplate reader ( Scott, G, J. Clin.Microbial . , 36, pp 362-366, 1998).

The experimental results are shown in Table 1 below. As can be seen in Table 1, the herbal doenjang of the present invention showed a cell growth inhibition rate of 50% or more with respect to MS 1, a mouse pancreatic cell line, at 7.6 to 12.6 µg / ml, and human blood cancer cells at 3.1 to 9.6 µg / ml. 50% cell growth inhibition rate was shown for the HL60 cell line, it was found that the herb miso of the present invention has an excellent inhibitory effect on cancer cell growth.

Concentrations of herbal doenjang of the present invention show a cell growth inhibition rate of 50% or more for each cell line division MS 1 cell line HL60 cell line Control (Camtotechin) 0.36 0.32 Comparative Example 1 37.4 27.6 Example 1 8.6 7.6 Example 2 9.7 9.0 Example 3 11.8 4.3 Example 4 10.3 5.9 Example 5 8.9 6.3 Example 6 7.9 7.6 Example 7 12.6 9.6 Example 8 7.6 3.1

Experimental Example 2: Investigation of weight loss effect and bowel movement promoting effect

Among five male and female volunteers with constipation and obesity, five randomly selected experimenters of the same height (male: 158cm, female: 145cm, female: 175cm, female: 30cm: 160cm) , After measuring the weight change and constipation improvement after eating the herb soybean paste prepared in Examples 1 to 8 and the doenjang prepared in Comparative Example 1 three times a day for breakfast, lunch and dinner with a meal for one month The results are shown in Tables 2 to 10 below. At this time, no drugs other than this herbal doenjang were taken.

Weight loss and bowel movement promoting effect of rosemary herb miso division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 77.4 4 days 66.7 1 day female 68. 4 days 57.4 1 day 30 spaces south 107.8 4 days 92.9 1 day female 87.3 4 days 74.1 1 day NOTE Each value is the average of five values.

Weight loss and bowel movement promoting effect of the lavender herb miso of the present invention division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 78.9 4 days 68.9 1 day female 67.9 4 days 57.1 1 day 30 spaces south 108.4 4 days 93.4 1 day female 89.4 4 days 75.7 1 day NOTE Each value is the average of five values.

Weight loss effect and bowel movement promoting effect of the present invention mint herb miso division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 77.8 4 days 68.1 1 day female 67.3 4 days 59.0 1 day 30 spaces south 111.3 4 days 96.4 1 day female 89.4 4 days 75.9 1 day NOTE Each value is the average of five values.

Weight loss and bowel movement promoting effect of chamomile herb miso division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 77.3 4 days 68.1 1 day female 66.7 4 days 57.4 1 day 30 spaces south 107.6 4 days 92.1 1 day female 88.5 4 days 75.2 1 day NOTE Each value is the average of five values.

Weight loss and bowel movement promoting effect of the sage herb miso of the present invention division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 77.2 4 days 69.3 1 day female 67.9 4 days 58.7 1 day 30 spaces south 110.3 4 days 96.7 1 day female 88.9 4 days 74.0 1 day NOTE Each value is the average of five values.

Weight loss and bowel movement promoting effect of the oregano herb miso division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 79.4 4 days 70.5 1 day female 66.7 4 days 58.6 1 day 30 spaces south 112.3 4 days 95.6 1 day female 87.6 4 days 72.1 1 day NOTE Each value is the average of five values.

Weight loss and bowel movement promoting effect of the present invention time herb division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 72.4 4 days 64.6 1 day female 66.7 4 days 58.7 1 day 30 spaces south 108.9 4 days 94.6 1 day female 84.6 4 days 71.9 1 day NOTE Each value is the average of five values.

Weight loss and bowel movement promoting effect of the present invention mixed herb miso prepared in Example 8 division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 76.3 4 days 60.4 1 day female 66.9 4 days 53.7 1 day 30 spaces south 111.7 4 days 91.4 1 day female 86.7 4 days 68.5 1 day NOTE Each value is the average of five values.

Comparative Example 1 Weight loss effect and bowel movement promoting effect of Doenjang division Before drinking After drinking Weight (kg) Average bowel movement Weight (kg) Average bowel movement 10 south 77.6 4 days 73.1 3 days female 65.7 4 days 60.7 2 days 30 spaces south 107.8 4 days 102.4 2 days female 88.3 4 days 83.9 3 days NOTE Each value is the average of five values.

As can be seen from Table 2 to Table 10, the herbal doenjang of the present invention was confirmed to have an excellent constipation improvement effect and excellent weight loss effect of 100%, it was found that the effect is more excellent in the specially mixed herb miso. .

Experimental Example 3: sensory test

The sensory test on the herb miso prepared in Examples 1 to 8 and the miso of Comparative Example 1 was performed as follows. The results are shown in Table 11 below.

The sensory test was performed by randomly extracting 10 men and women aged 20 to 50 years to obtain a nine-point grading system (9 = best; 8 = very good; 7 = usually good) for taste, taste, color, and texture. Good; 6 = Slightly good; 5 = Not good or hate; 4 = Slightly disliked; 3 = Normal dislikes; 2 = Very disliked; 1 = Most disliked; The average value was calculated | required.

Sensory test results of the herb miso of the present invention division Comparative Example 1 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 incense 2.6 7.6 8.6 8.2 7.7 7.9 8.4 8.4 8.6 flavor 4.6 7.6 7.9 8.0 7.9 8.6 8.3 7.9 8.4 color 2.9 8.6 7.6 7.0 7.6 7.9 8.1 8.2 8.6 touch 4.6 7.3 7.4 7.6 8.6 8.0 7.6 7.4 8.0 Overall preference 3.7 7.8 7.9 7.7 8.0 8.1 8.1 8.0 8.4

As can be seen in Table 11, the herbal doenjang of the present invention was found to be more preferred by consumers in terms of sensuality as compared to the general miso of Comparative Example 1. Therefore, the herb miso of the present invention was expected to be excellent in the purchase symbol in the sales market.

As described above, the present invention is obtained by mixing the extract obtained by low-temperature aging and then extracting the herbal plants, and the shoots and herb flowers mixed with rock water to go to a blender and then mixed with a fresh juice obtained by low-temperature aging It is a very useful and groundbreaking invention in the food industry because it can provide a herb miso prepared with a mixture of excellent functional beauty and anti-cancer functionality.

Claims (3)

The herb plant was vacuum-freeze-dried at -70 ° C to -90 ° C to extract dried raw material at 40-50 ° C for 1-3 hours, and then aged at low temperature at 2 to 10 ° C for 1 hour to 3 days to prepare an herbal extract. step; Mixing sprout sprouts and herbal flowers with natural rock water, going to a blender, and then aging at 2-10 ° C. for 1 hour to 3 days to produce a fresh juice of sprout sprouts and herbal flowers; Mixing 0.1-10 parts by weight of the juice solution of the step with respect to 1 part by weight of the herbal extract of the step; Method of producing a herb miso characterized in that the fermentation of 0.1-10 parts by weight of the mixed solution of the herbal extract and the juice of the step to the total weight 100 parts by weight of the miso made and then fermented. The herbaceous plant of claim 1, wherein the herbaceous plant is rosemary, lavender, mint, chamomile, sage, oregano, thyme, lemongrass, rosehip, echinacea, jacquard, sweet fennel, mate, yukari, ginger, chamomile, stevia, jasmine Method of producing a herb miso, characterized in that any one or more selected from the group consisting of, lilac, orange flower, eye bright, orchid, and mauve. A herb miso which is characterized by excellent functionality and anti-cancer functionality prepared by the method according to claim 1 or 2.