KR100487887B1 - East extract relaxing premenstrual sydrome and menstrual pain, compositions containing the same, and preparation thereof - Google Patents

Abstract

The present invention relates to a composition useful for improving premenstrual syndrome (PMS) and alleviating dysmenorrhea in women, and more particularly, to a yeast extract obtained by hydrolyzing a yeast approved as a health functional food material. As a main raw material, chitosan, Angelica, sweet ginseng, turmeric, licorice, extract, peppermint, cinnamon, peppermint, ginger, ginseng, Cheonma, Cheongung, Baekchul, and other herbal ingredients are added and mixed, and these are ingested in the form of capsules or tablets, The present invention relates to a composition useful for improving premenstrual syndrome and relieving menstrual cramps for women, characterized in that it is ingested in the form of a beverage and a method of manufacturing the same.

This new use of yeast extract is a PMS or dysmenorrhea in everyday life by allowing the user to easily purchase and consume yeast extract as a food that has no resistance or side effects and is safe in the PMS or dysmenorrhea without a doctor or pharmacist's prescription. It can be easily alleviated.

Description

Yeast extract having the function of premenstrual syndrome and dysmenorrhea relief, a composition comprising the same, and a method for preparing the same. {East extract relaxing premenstrual sydrome and menstrual pain, compositions containing the same, and preparation}

The present invention relates to a composition useful for improving premenstrual syndrome (PMS) and alleviating dysmenorrhea in women, and more particularly, to a yeast extract obtained by hydrolyzing a yeast approved as a health functional food material. Improvement of premenstrual syndrome in women characterized by adding and mixing herbal ingredients such as chitosan, Angelica, Salvia, Salvia, Licorice, Licorice, Mint, Cinnamon, Peppermint, Ginger, Ginseng, Cheonma, Cheongung, Baekchul And compositions useful for alleviating menstrual cramps and methods of making the same.

Premenstrual syndrome (PMS) refers to physical and mental abnormalities that occur during the menstrual cycle after ovulation in women of childbearing age, associated with the menstrual cycle of women. Physical symptoms include abdominal pain, back pain, pelvic pain, abdominal distension, diarrhea, constipation, and stiff breasts. Mental symptoms include anxiety, irritability, depression, insomnia, fatigue, loss of concentration, and suicidal thoughts. These symptoms account for 70% of the female population, 20% of which are serious and the social and economic losses caused by women's loss of activity are also significant.

In addition, dysmenorrhea, which is experienced by most women with premenstrual syndrome, refers to lower abdominal pain or low back pain that occurs lightly or severely during or after menstruation. Menstrual pain is one of the most common gynecological disorders in about 50% of women of childbearing age. Menstrual pain usually occurs in young women, usually within one to two years after menarche, but severe cases can last up to the age of 40. About 10% of them complain of pain that they can't do their usual work for one to three days every month. A survey by Kyunghee Medical Center Oriental Medicine Hospital showed that 47% of women complained of pain such as menstrual abdominal pain, low back pain and even headache, 13% of gastrointestinal disorders such as anorexia and indigestion, and 8% of women who felt unpleasant. In particular, 70% of the 632 high school girls complained of dysmenorrhea and 50% complained of severe pain. More pain in female students was attributed to excessive academic and test fears. However, 66% of them just refer to 28% of painkillers, and more than 90% of them do not have a basic treatment.

The exact cause of PMS or dysmenorrhea has been studied by many scholars for a considerable period of time, but the exact cause is still unknown. However, the fact that PMS or dysmenorrhea is associated with the menstrual cycle suggests that the endocrine hormone imbalance is deeply related.

There are several theories regarding the pathogenesis of PMS and dysmenorrhea, including the theory that the progesterone deficiency is the cause, the theory that the excessive secretion of estrogens and androgens is the cause, the theory that the excessive production of pain-producing prostaglandins, There is a theory that nutritional deficiency such as vitamin B complex or essential fatty acid is the cause, and in general, it is thought that PMS and dysmenorrhea occur because the above factors are combined or environmental factors are added. have.

Of these theories, PMS or dysmenorrhea occur due to excessive secretion of prostaglandins, the most prominent theory of which is a pain-inducing substance when the release of progesterone (progesterone) is stopped in the secretion of female hormones along the menstrual cycle. Prostaglandins are secreted and the uterine muscles are severely reduced and ischemia causes pain. Therefore, the following methods are used to suppress such pain and various PMS symptoms.

The most common method is pain relief by inhibiting the production of prostaglandins using aspirin, ibuprofen, etc. Another method is to use anti-anxiety, anti-depressant agents such as benzodiazepines, and another method is the corpus luteum of the menstrual cycle. There is a method of administering progesterone in the formation step.

Further known treatments include the method of administering melatonin (N-acetyl-5-methoxytrytamine) disclosed in Korean Patent No. 0171408, and serotonin (5) disclosed in Korean Patent Application Laid-Open No. 2001-0024462. Hydroxytryptamine).

Of these methods, methods of administering hormones or neurotransmitters such as progesterone, melatonin, and serotonin during the corpus luteum formation phase are known to improve not only PMS or dysmenorrhea, but also general pain relief and chronic fatigue, depression, and the like.

However, since the above methods directly administer hormones, melatonin, and serotonin, they cannot be 100% convinced of their safety, and have high manufacturing costs, so they cannot be easily taken at all times.

Therefore, in the present invention, instead of administering hormones such as progesterone, melatonin, serotonin, and the like, a composition and a method for preparing the composition and a method for producing a PMS or dysmenorrhea relief effect using a commercially available and proven food material as a raw material It aims to provide.

In order to achieve the above object, in the present invention,

It provides a yeast extract having PMS and dysmenorrhea relief, characterized in that it is extracted from yeast. At this time, the yeast is preferably autodigested at a temperature of 35 ° C to 70 ° C, and it is preferable to hydrolyze the protein with proteolytic enzymes simultaneously with or autodigesting the yeast. At this time, only the supernatant can be taken by centrifuging the hydrolyzed yeast.

In addition, the present invention to achieve the above object,

It provides a composition for relieving PMS and dysmenorrhea, characterized in that it comprises 10 to 90% by weight of the dried yeast extract, 5 to 80% by weight of chitosan, and 5 to 80% by weight of the herbal material powder. In this case, the chitosan is preferably a water-soluble polymer chitosan having a molecular weight of 300,000 or more, and the herbal material is a group consisting of Angelica, Salvia, Curry, Bacteria, Mint, Licorice, Ginger, Cinnamon, Baekchul, Cheongung, Cinnamon and Ginseng It is preferably one or more selected from.

In addition, the present invention to achieve the above object,

Culturing the yeast strain to the maximum growth phase;

Hydrolyzing the cultured yeast at a temperature of 35 ° C. to 70 ° C. while simultaneously adding more proteolytic enzymes to hydrolyze the yeast protein; And

It provides a method for producing a yeast extract having a PMS and dysmenorrhea function comprising the step of centrifuging the hydrolyzed to separate only the supernatant. In the method, the method further comprises the step of inducing the secretion of metabolites to withstand stress by applying appropriate physical or chemical stresses such as high temperature, ultrasonic wave, vibration, pH change, etc. in the range that yeast does not die before self-extinguishing the yeast. Preferably, the obtained supernatant further comprises spray drying.

In addition, in order to achieve the above object, the present invention provides a functional beverage for alleviating PMS and dysmenorrhea, comprising the above-mentioned yeast extract.

Hereinafter, the present invention will be described in more detail.

In one aspect of the present invention, there is provided a yeast extract having premenstrual syndrome and dysmenorrhea function.

Yeast has been commercially produced and used as a raw material for microbial fermentation media, seasonings and dietary supplements (Roman et al., Food Biotechnology, 6, 225, 1992). Yeast extract is a dietary supplement and nutritional imbalance. It is known to be used for improvement, nutrition, health promotion and maintenance, metabolic activity, etc. (Refer to the indications of use by food type of Korean health supplement food and special nutrition food association). I just lost.

Therefore, the present invention has been completed to the present invention by uncovering the alleviation effect of PMS and dysmenorrhea, which is a new use of yeast extract that has not been identified so far.

The yeast extract of the present invention is prepared by the same method as the production method of the yeast extract described in the applicant's first filed patent application No. 01-9946.

As found in the preceding application, the yeast extract prepared by the above method contains more than 50% of high-quality protein having anti-stress function, anti-fatigue function and brain neurotrophic factor, and is easy to lack in the human body. Rich in minerals and vitamins. In addition, the yeast extract is degraded by self-digesting enzymes and other proteolytic enzymes in the form of low molecular peptides, the bioavailability is very high.

The present invention provides a relieving effect of premenstrual syndrome and alleviation of dysmenorrhea, which is a new use that has not yet been identified in the yeast extract as described above.

The effect of the yeast extract of the present invention on the relief of premenstrual syndrome and dysmenorrhea can be explained as follows.

First, the vitamin and amino acid composition contained in the yeast extract of the present invention were analyzed as follows.

The amino acid composition is 10 g of the yeast extract of the present invention dehydrated with cold acetone and then dried on a filter paper in a 60 ° C. dry oven, followed by 5 mg of a sample in a hard test tube, 5 ml of 6 N HCl, degassed, and sealed to 110 ° C. Hydrolyzed for 24 hours, washed with a small amount of distilled water 2-3 times, dried at 50 ° C. with a concentrator to remove hydrochloric acid, and dissolved in buffer to exchange column Li 10cm No.338051 with amino acid automatic analyzer (Deckman system 6300, USA). It was analyzed using. The results are shown in Table 1.

Vitamin composition was analyzed based on the trace component analysis method contained in the Republic of Korea food additives, the results are shown in Table 2.

Amino acid types Content (% of protein) Aspartic acid 5.0 Glutamic acid 14.2 Serine 2.8 Glycine 3.0 Histidine 4.3 Arginine 2.3 Threonine 3.0 Alanine 4.7 Proline 2.0 Tyrosine 3.5 Valine 3.4 Methionine 1.5 Cysteine 0.8 Isoleucine 3.9 Leucine 6.9 Phenylalanine 3.7 Lee Sin 5.2 Tryptophan 1.4

Vitamin types Content (mcg / g) Thiamine 50 Riboflavin 35 Niacin 550 Pyridoxine 25 Choline 2000 Biotin 2 Inositol 3000 Pantothenic Acid 100

As can be seen from the above composition, the yeast extract of the present invention contains all the amino acids including essential amino acids and contains a large amount of vitamin B group and is not indicated in the table, but according to the existing reports, yeast is selenium, calcium, chromium and magnesium It is known to be rich in various minerals.

In women with premenstrual syndrome or those with severe dysmenorrhea, progesterone deficiency or estrogen / progesterone hormone imbalance, salt and water stagnation, prostaglandin deficiency or excess, excess prolactin, and vitamin B ₆ (pyridoxine) Deficiency, hypoglycemia, and serotonin deficiency have been found. As described above, the yeast extract of the present invention is rich in vitamin B group including various amino acids and vitamin B ₆ and minerals such as calcium and magnesium. When absorbed into the body, it can quickly activate hormone balance and metabolism.

By the way, a known method for the treatment of premenstrual syndrome, that is, a method of administering progesterone in the corpus luteum formation phase of the menstrual cycle, a method of administering melatonin (N-acetyl-5-methoxytrytamine) (Korean Patent No. 0171408 No.), and a method of administering serotonin (5-hydroxytrypamine) (Korean Patent Publication No. 01-24462), etc., synthesize the above substances separately and add vitamin B ₆ , magnesium, etc. It was to be constructed and manufactured. On the other hand, the yeast extract of the present invention, as shown in Table 1 and Table 2, because it contains all of the hormonal balance control components that are easy to be deficient in itself, there is no need to purchase and mix separately, peptides derived from yeast Because of this, there is an advantage that the bioavailability is also excellent. Therefore, the present inventors have invented a dietary supplement composition useful for alleviating premenstrual syndrome and dysmenorrhea in women whose yeast extract is the main ingredient.

More detailed reasons why yeast extracts affect PMS relief and menstrual pain relief are as follows.

First, choline (choline) contained in a large amount of yeast extract is a vitamin B complex, a precursor that produces acetylcholine. Acetylcholine is a neurotransmitter that promotes relaxation and relaxation. It can relieve muscle tension caused by contraction of the uterus, which can have an analgesic effect.

In addition, choline has several types of choline, among which salicylic acid choline has an analgesic effect similar to that of aspirin, and thus may exhibit analgesic effect of menstrual pain. The cause of dysmenorrhea is not yet known correctly, presumably because women with dysmenorrhea are more prostaglandins released during menstruation than women without menstrual pain. At this time, painkillers such as acetylsalicylic acid (aspirin) or ibprofen prevent the prostaglandin from being synthesized, thereby reducing the amount of prostaglandin released and preventing pain from being delivered.

Second, vitamin B6 in the yeast extract composition plays a very important role in the synthesis and metabolism of amino acids and proteins, plays a role in making red blood cells and antibodies, and is involved in the production of various neurotransmitters. Therefore, vitamin B6 deficiency tends to be anemia, dermatitis, neurological disorders, and convulsions because it can show the symptoms of premenstrual syndrome and dysmenorrhea is essential vitamin. This vitamin is already known to be effective in relieving premenstrual syndrome and dysmenorrhea.

Third, in the composition of yeast extract, niacin is involved in the enzymatic reaction of making two coenzymes of NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide phosphate), which promotes energy production in the mitochondria of cells and promotes the function of brain cells. It is known that it can be used to treat schizophrenia by playing a role in promoting. Therefore, it can be effectively used for mental abnormalities of PMS.

In addition, since niacin is one of the normal metabolites of tryptophan, an essential amino acid, and tryptophan is a precursor of serotonin, which is known to be used for PMS and dysmenorrhea, yeast extracts containing large amounts of tryptophan and niacin are effective in relieving PMS and dysmenorrhea. Can not help That is, since serotonin is an intrinsic atmosphere-regulated sedative neurotransmitter that is synthesized in vivo, many structurally similar niacin is likely to convert to serotonin, and thus the body's own mechanisms for treating pain, chronic fatigue, and depression. Can be said to maximize.

Fourth, thiamine in the composition of yeast extract is involved in the nervous system, such as in the normalization of the heart and nervous tissues, so if the blood thiamine levels are low, depression, emotional anxiety, sleep disorders, irritability. It has been shown to cause hyperactivity, and that thiamine administration eliminates these symptoms (Professor Rus Harrell, University of Columbia, New York). Therefore, by taking thiamine-rich yeast extract, it can help to treat women's PMS symptoms such as depression, emotional anxiety, sleep disorders and irritation.

Fifth, riboflavin is involved in the synthesis of coenzymes such as flavin mononuc-leotide (FMN) and flavin adenine dinuleotide (FAD) in the composition of yeast extract. Participate in your back. Therefore, nutrients are essential for physiological bleeding.

Sixth, selenium, a mineral contained in yeast extract, is 1,900 times larger than antioxidants. In other words, if the neurotransmitters, including serotonin produced in the human body, are oxidized in the gastrointestinal tract or during the delivery process, the effect is reduced. Therefore, the presence of selenium having an antioxidant effect that prevents the neurotransmitters from oxidizing, Neurotransmitters help to get the most out of the effect.

Lastly, menstrual cramps require a lot of minerals such as calcium and magnesium. These minerals must be absorbed as foods for quick absorption into the living body. Therefore, yeast extract can be easily absorbed by the human body because it is rich in both minerals and amino acids, it can exhibit a useful effect in relieving PMS and dysmenorrhea.

By the above mechanism, it can be seen that the yeast extract is very effective for the relief of PMS and dysmenorrhea.

In still another aspect of the present invention, there is provided a composition having an alleviating effect of premenstrual syndrome and dysmenorrhea including chitosan in addition to the yeast extract.

Chitosan is a very good substance that boosts autoimmunity, helps calcium absorption and helps regulate cholesterol. Calcium is an essential mineral for women, especially premenstrual syndrome and menstruation. However, because chitosan aids in the absorption of calcium and is also excellent for removing foreign substances, it is useful for the relief of premenstrual syndrome and dysmenorrhea. The chitosan used in the present invention is preferably a water-soluble high molecular chitosan having a molecular weight of 300,000 or more.

In still another aspect of the present invention, in addition to the yeast extract and chitosan, herbal ingredients such as donkey, sweet ginseng, turmeric, licorice, cinnamon, peppermint, ginger, ginseng, cheonma, sweet ginseng, cheongung, baekchul, and buds may be further included. A composition having a palliative effect of premenstrual syndrome and dysmenorrhea can be provided.

Angelica, Salvia, Licorice, Turmeric, Peppermint, Ginseng, Cinnamon, Ginger, etc. are good herbal medicines for women, especially known to help blood circulation, blood removal, pain relief, appetite and metabolic functions (see herbal medicine). During menstruation, blood circulation is not smooth, so congestion occurs and often suffers from dysmenorrhea, such herbal ingredients can alleviate such dysmenorrhea.

In addition, cheonma, cheongung, baekchul, bleeding, etc. helps the blood circulation of the brain and clear the head, can help the production of neurotransmitters.

The above materials are all powdered and used, and the blending ratio is preferably 10 to 90% by weight of yeast extract, 5 to 80% by weight of chitosan, and 5 to 80% by weight of herbal material.

When a large amount of yeast extract is added, it may be good for a patient who feels a lot of pain, but when taken in a patient with mild pain, it may be rather relaxed, so the content of the yeast extract needs to be appropriately adjusted within the above range.

In addition, when a large amount of chitosan is used, the body has a lot of irritation due to the acidity of chitosan, which causes severe irritation in the body. It can act as a rather overwhelming body.

However, at least 10% by weight yeast extract and at least 5% by weight of chitosan should be included to induce the proper metabolism by inducing the interaction of the relaxation and stimulation of the yeast extract and chitosan while utilizing each active ingredient.

In addition, the maximum content of yeast extract and chitosan at 90% by weight and 80% by weight, respectively, is similar to the above conditions that cause the interaction of the minimum weight ratio, which causes proper interaction between relaxation and stimulation of the yeast extract and chitosan. To act as an effective composition.

In addition, the 5% minimum weight ratio of herbal ingredients helps to eliminate blood clots that have not been completely removed during menstruation, so adding herbal ingredients at least 5% weight ratio alleviates desirable dysmenorrhea and premenstrual syndrome. This is because the composition for manufacture can be prepared.

PMS and dysmenorrhea yeast extract prepared in the present invention and a composition comprising the same are all powdered and filled into capsules or in the form of a definition, 1-2 capsules (or tablets) once or twice a day (2-3 times a day) 1 capsule (tablet) 180mg) with a large amount of water is effective in the treatment of women's premenstrual syndrome, dysmenorrhea, cold and dysmenorrhea.

In addition, the yeast extract prepared in the present invention and the composition comprising the same can be used for health promotion of women who contribute to the relief of premenstrual syndrome, dysmenorrhea, cold symptoms and the like by being prepared as a health drink.

Hereinafter, the manufacturing method of the composition of this invention is demonstrated in detail.

The method for preparing a yeast extract, which is a main component of the composition of the present invention, comprises the step of separating and purifying the autolyzed yeast at a temperature of 35 ° C. to 70 ° C. and hydrolyzing it again with a protease, wherein PMS and The reason why the yeast extract having a menstrual pain relief effect is prepared in this way is as follows.

In other words, the temperature stimulus and pH stimulus applied to increase the decomposition efficiency when the yeast is self-digested or decomposed using a hydrolase may increase the efficiency of degradation. It can be seen that the process of applying stress, such as pH.

'When all living organisms perceive stress for the first time, a physiological arousal response that mobilizes body resources against stress appears, and when stress continues, the organism enters the resistance stage and proceeds to suppress the stress. In the light of Seyle, 1956), yeast also shows a corresponding response to external stimuli as a living body, in which the physiological direction in the direction of suppressing the stimulus against external stimuli such as temperature, vibration, pH, etc. Will cause a change. As a result, they secrete large amounts of hormones such as mating pheromones or secrete unknown antistress agents.

Therefore, ultrasonic wave, vibration, pH change, temperature change, etc. may be artificially applied in the process of preparing yeast extract to release a large amount of antistress substances beneficial to the human body.

There is a recent report that acupuncture in the human body improves disease because stimulation such as saliva stimulates the release of nerve hormones such as endorphins (American Journal of Physiology June 1999). In the presence of calcium ions, treatment with electrical stimulation or high concentrations of potassium ions caused the release of neurotransmitters or neuropeptides in the medial ridge of the hypothalamus (Neurohormone, Kyung-Jin Kim, Hyung-Seop, Daewoo University, In reference to p23, 2000, yeast, a primitive independent single cell form, can be similarly secreted by neurohormones by various stimuli such as high temperature, pH change, ultrasound, and vibration.

Supporting this fact is that polypeptides similar to mammalian neuropeptides are found in lower vertebrates as well as in the early evolutionary single-celled organisms, yeast and bacteria. In particular, the α-factor, a mating pheromone in yeast, is structurally similar to GnRH (gonadotropin / releasing hormone), a mammalian neurohormone, as described below. [Neurohormone] Kyung-Jin Kim, Cho-Hyung, Daewoo Academic Journal, 2000 / Stotzler, D., Kiltz, H.-H & Duntz, W. (1976) Eur. J. Biochem. 69, 397-400)

α ₁ -factor (yeast)

H-Trp- His- Trp -Leu-Gln -Leu-Lys- Pro- Gly -Gln-Pro-Met-Tyr-OH

GnRH

PGlu - His- Trp -Ser-Tyr- Gly-Leu-Gln- Pro- Gly -NH2 ( mammals)

PGlu - His- Trp -Ser-Tyr- Gly-Leu- Arg - Pro- Gly -NH2 ( chicken)

PGlu - His- Trp -Ser-Tyr- Gly-Leu-Ser- Pro- Gly -NH2 ( black sea bream)

Furthermore, the stimulus applied in the liquid medium in which the live yeast grows is much more effective than the multicellular organisms, so the stimulus in the liquid medium is more effective than the single cells that have evolved into multicellular organisms. It can be said.

Therefore, the addition of this stimulating process can produce a more functional yeast extract.

In addition, many peptides detected in the digestive tract are found in the brain tissue, and neurohormones and peptides known to be distributed only in the nervous tissue are found in the endocrine system, which is a non-neural tissue such as the digestive tract and skin. It suggests that neurotransmitters, neuropeptides and hormones are all derived from one cell regulator that was present in primitive unicellular organisms. (Neurohormone, Kyung-Jin Kim, Se-Hyung Jo, Daewoo Medical Research, 2000 p20)

 Therefore, the yeast extract of the present invention, which contains various secretory substances (exoenzyme) secreted due to the stimulation to the yeast, a primitive unicellular organism, acts as a neurotransmitter to effectively relieve pain such as PMS or dysmenorrhea in the human body. can do.

Example

Although the present invention will be described in more detail with reference to the following examples, the examples should not be construed as limiting the present invention, and conventional variations of those skilled in the art are possible within the scope of the present invention.

Example  One

Food grade yeast Saccharomyces cerevisiae strain (purchased by Genico Co., Ltd.) was incubated in YM medium at 24 ° C. and oxygen for 48 hours.

After centrifuging the culture broth as described above for 15 minutes at 15,000 rpm, the supernatant was removed, and the remaining precipitate, ie, the yeast was washed and diluted twice with 10 times (v / v) sterilized water, and then the yeast was heated at a high temperature of 50 ° C. In addition to self-digestion using an autolysis enzyme that dissolves itself, 1% of protease (papaine 30.000) was added to facilitate the breakdown of yeast proteins into peptides. Time hydrolysis was performed to dissolve the protein into peptides.

The dissolved solution was centrifuged at 15,000 rpm to separate only the supernatant in an aqueous solution and spray dried.

In addition, powdered Angelica, Salvia, Turmeric, Bulgogi, Mint, and Ginseng at the same weight ratio, respectively, and then mixed well to prepare a herbal medicine material.

In addition, chitosan prepared the water-soluble high molecular chitosan (purchased from the light magnetic chitosan Co., Ltd.) whose molecular weight is 300,000 or more.

The ingredients prepared as above were mixed at a ratio of 40% by weight of yeast extract, 30% by weight of chitosan, and 30% by weight of herbal medicine, and then filled into capsules to make 180 mg of 1 capsule.

Example  2

Food-grade Saccharomyces from KCTC The cerevisiae 7904 strain was incubated for 48 hours in a fermenter at 24 ° C. and oxygen supply in YM medium. The culture solution was centrifuged at 5,000 rpm for 15 minutes to remove the supernatant, and the remaining precipitate, yeast paste, was treated with 1% peptone buffer. After diluting 10 times, in the fermentation tank equipped with the ultrasonic generator and the vibrator, the high temperature of 35 ℃ to 45 ℃ and the ultrasonic wave of 45Khz for 30 seconds are repeated for 5 seconds and the drop by using the water pump While giving a vibration using the yeast is incubated for 8 hours while generating a lot of production of anti-stress substances secreted by the stress.

The cultured culture was subjected to self-extinguishing using a self-extinguishing enzyme in which yeast dissolves itself at a high temperature of 50 ° C. In addition, 1% protease (papaine 30.000) was added to promote the hydrolysis of the yeast protein, and the protein was dissolved in a low molecular peptide by 48 hours of hydrolysis at an initial pH of 4.0.

As described above, the autodigested and hydrolyzed was centrifuged at 15,000 rpm to separate only the supernatant in aqueous solution and spray dried to prepare a yeast extract.

Powdered Angelica, Sweet Ginseng, Turmeric, Bulgogi, Mint, Licorice, Ginger, Cheonma, Baekchul, Cheongung, and Ginseng were each powdered in the same weight ratio, and then mixed well to prepare herbal ingredients.

In addition, chitosan was prepared with the same kind of chitosan as in Example 1.

The ingredients prepared as above were mixed at a ratio of 70% by weight of yeast extract, 10% by weight of chitosan, and 20% by weight of herbal medicine, and then filled into capsules to make 180 mg of 1 capsule.

Experimental Example 1 (Clinical Experiment by Hwang Won-Joon Neuropsychiatrist)

The capsules prepared according to Example 1 were given to 30 women aged 20-35 years, 1 to 2 capsules once a day before menstruation or during menstruation with menstruation, especially for women with premenstrual syndrome, dysmenorrhea and coldness. It was taken 2-3 times.

The survey was conducted on various changes resulting from the application. At this time, 30 items of total change evaluation table were selected and 10 items of physical symptoms, 10 items of emotional symptoms, and 10 items of behavioral symptoms were composed. The 30 survey questions were as follows.

A) somatic symptoms

1) Feeling weak or tired

2) appetite increases or decreases.

3) The breast is swollen or sick.

4) Head hurts

5) Masquerade or vomit.

6) dizzy or dizzy.

7) Your body is swollen.

8) The stomach is sore or uncomfortable and there are intermittent abdominal cramps.

9) I have insomnia.

10) Sexual activity or interests change.

B) emotional symptoms

1) There is a rapid change of emotions in one day.

2) The mind is uneasy and restless.

3) The nerves become sensitive or calm.

4) Sad or depressed

5) Nagging or arguing about trivial matters.

6) If the stomach is damaged, it becomes hysterical.

7) I feel guilty.

8) Less understanding of others' faults or mistakes (or impatient)

9) Think about or worry about unpleasant events.

10) Blunt

C) behavioral symptoms

1) The movement becomes dull or clumsy.

2) Make mistakes or accidents (falls, cuts or accidental damage)

3) Think about death or suicide (or don't want to fall asleep and wake up forever)

4) I don't want to talk or wander around.

5) Use drug abuse (stimulants, sedatives, etc.) (or smoke or smoke a lot).

6) Be violent or barking.

7) Reduces ability, activity, and social participation in the home or workplace.

8) Avoid social activities and want to be alone in the house.

9) Reduce household chores (cleaning, laundry, etc.).

10) Less time spent on leisure activities (hobbies, watching TV, reading, etc.).

For the above questions, depending on the severity of the change, there were 1 symptom with no symptom, 2 with little symptom, 3 with some symptom, 4 with very severe, 5 with very severe, and 6 with very severe. Was divided into before and after ingestion, and the time and duration of pain relief effect were investigated. In this way, the total number of individual surveys was added to calculate the average.

As a result, pain relief was effective within 10 minutes after ingestion at least 1 hour, and the duration was about 4-6 hours, and the degree of change was menstruation in all areas of body, emotion and behavior. It has been shown that the symptoms due to the improvement.

The average degree of change is shown in the table below.

Before ingestion After ingestion The degree of change Physical symptoms 32 23 9 Emotional symptoms 30 24 6 Behavioral symptoms 29 22 7 Gross symptoms 91 69 22

Experimental Example  2

For women of childbearing age between 20 and 35 years of age to confirm the pain relief and other effects during menstruation for the composition of the present invention, without taking an example of a questionnaire, symptoms before taking, time to take after taking, Post-dose effects and duration of post-dose effects were described directly.

Dosing time was 180 mg (1 capsule) containing the composition of Example 1 at the time of pain comes to take 1-2 tablets daily.

The opinions of the women who responded to the survey were as follows.

1. A 29-year-old female worker reported that the symptoms of menstruation before taking the composition of the present invention were pain in the lower abdomen, indigestion, cold sweating, poor circulation, and limbs. However, about 20-25 minutes after taking it, she could sleep comfortably and wake up after waking up. When taking general pain medication, the face was swollen after taking it, and the stomach was not good, but when taking the capsule containing the composition of the present invention, the pain was eliminated, but there was no such side effect.

2. A 32-year-old unmarried female worker had a severe back pain and discomfort in her lower stomach before taking the composition of the present invention at menstruation, but after about 30 minutes after taking two capsules of the composition of the present invention I could feel relief.

3. A 30-year-old unemployed female researcher reported that she had difficulty digesting and bulging her abdomen a day before menstruation, and continued diarrhea at the beginning of menstruation. In addition, my stomach became cold and depressed and sensitive and irritable. However, about 30 minutes after taking it, the pain in the lower abdomen has subsided, the body feels better and the stomach feels better and less irritable. This effect lasted for about half a day for more than 4-5 hours and did not have diarrhea.

4. A 33-year-old married woman who was a broadcast writer had severe dysmenorrhea so that her first day of menstruation was inactive. After taking 4 capsules of 2 capsules 2 times a day before the start of menstruation, the pain was relieved enough to be active during menstruation and there was no discomfort. This effect lasted for two days.

5. The menstrual symptom of a 32-year-old female worker is not as bad as pain medication, but her stomach hurts and her stomach feels bloated and her appetite decreases. About 40 minutes after taking this capsule, the pain disappeared and my stomach aches and pains disappeared and I felt refreshed and feeling better.

6. A 32-year-old official said she had dizziness, dizziness, and dizziness in her menstruation during menstruation, and her symptoms were alleviated about 40 minutes after taking this capsule. The effect seemed to last about 4-5 hours, and the anemia felt to be aging.

7. A 28-year-old unmarried woman who is on a translation day does not have severe menstrual pain, but she feels depressed before she starts menstruation, causing her to become irritable and sensitive. But after 40-50 minutes after taking this capsule, some menstrual cramps disappeared, my bloated stomach became more comfortable, and I felt better. The duration of effect after taking it was about 3-4 hours.

8. A 32-year-old interior designer had a severe menstrual cramp so she always had to take painkillers, low menstrual blood, and cold hands and feet. But after 20-30 minutes after taking this capsule, the pain disappeared, the feeling was soft, the menstrual blood increased, and the bleeding pain disappeared. The effect began to appear 2-3 hours after taking it, and my stomach felt warmer and feeling better.

9. A 23-year-old college student had severe menstrual pain and had to take painkillers before starting menstruation and became cold. But after 15 to 20 minutes after taking this capsule, my stomach became warm and almost no menstrual pain. Duration of effect lasted 2-3 hours.

10. A 23-year-old female researcher did not usually have severe dysmenorrhea, but she was often irritated by nervousness. 20-30 minutes after taking this capsule, the nerves felt somewhat stable and the effect seemed to last about 5-6 hours.

11. A 38-year-old housewife suffered from severe menstrual cramps and was so painful that she decided to remove the uterus. After taking this capsule for about a week, she decided not to operate.

12. A 28-year-old teacher was severe enough to take 2-4 tablets of normal menstruation during her menstruation, but her pain was gone after taking this capsule and the effect lasted 4-5 hours.

The results of writing the personal opinions of the women who responded to the survey were as follows.

In other words, the composition of the present invention before or during menstruation, the pain is severe, the body is paralyzed, the food is not digested and appeared to work effectively to improve the symptoms of vomiting, improve blood circulation and improve mood appear. Most of the women who took the composition of the present invention said that the pessimistic and depressed mood before ingestion disappeared after ingestion. The composition of the present invention has been shown to be particularly effective for menstrual low back pain. Among women who participated in the post-dose evaluation questionnaire, women who suffered severely from menstrual low back pain after taking 2 capsules (180 mg / capsule) of the present invention at a time 30 Low back pain disappeared in a minute, he said, I hope you will eat again. In addition, the composition of the present invention can not sleep deeply due to menstrual cramps, and women who have woken up several times from menstrual cramps were able to sleep comfortably after ingestion. Furthermore, the composition of the present invention does not have an analgesic effect as numbness and numbness as a conventional analgesic, and some analgesic remains but not feeling bad, so rather than feeling lost, it is better than the analgesic effect. This was mostly. In addition, the composition of the present invention said that during menstruation, the hands and feet became cold and the abdomen became cold, and most women who consumed said that the abdomen and hands and feet were warmed. In particular, the composition of the present invention showed a greater effect in women who took before the start of menstruation, and when taken after the onset of pain, the effect appeared after an average of 3-4 hours. In addition, among the opinions of the women who answered the question, the pain relief agent is not good, but I just referred to the pain, but the composition of the present invention has a pain-relieving effect along with the health promoting effect, so that instead of the conventional analgesic medicine, the reaction to take the present invention composition is a food. Indicated.

Experimental Example  3

Although there were differences according to the degree of disease, five women who were in poor health were given long-term intakes for 10 to 50 days, resulting in excellent relieving effects such as premenstrual syndrome, dysmenorrhea, dysmenorrhea, low back pain, and cold symptoms. This weak woman was found to be effective in promoting women's health, such as finding health and vitality in life.

This new use of yeast extract replaces drugs that have been prescribed by doctors or pharmacists in PMS or dysmenorrhea, or certain products such as serotonin and melatonin, which replace yeast extracts as foods that have no resistance or side effects and are guaranteed to be safe. By making it easy to buy and consume without prescription, it is easy to relieve the pain caused by monthly PMS or dysmenorrhea among normal women who are difficult to call a patient.

1 is a flow chart schematically showing a yeast extract of the present invention and a method for producing a composition comprising the same.

Claims (11)

PMS and dysmenorrhea composition containing yeast extract as an active ingredient. According to claim 1, wherein the yeast extract PMS and dysmenorrhea composition, characterized in that the self-extinguishing yeast at a temperature of 35 ℃ to 70 ℃. According to claim 2, wherein the yeast extract PMS and dysmenorrhea composition, characterized in that hydrolyzing the protein with proteolytic enzymes at the same time or after auto-digesting the yeast. According to claim 3, wherein the yeast extract PMS and dysmenorrhea composition, characterized in that to take only the supernatant by centrifuging the hydrolyzed yeast. According to claim 1, wherein the yeast extract 40 to 70% by weight, chitosan 10 to 30% by weight, and the group consisting of Angelica, Salvia, Curry, Bacteria, Mint, Licorice, Ginger, Cheonma, Baekchul, Cheongung, Cinnamon and Ginseng PMS and dysmenorrhea composition comprising 20 to 30% by weight of one or more herbal medicine powder selected from. The composition for PMS and dysmenorrhea relief according to claim 5, wherein the chitosan is a water-soluble high molecular chitosan having a molecular weight of 300,000 or more. delete Culturing the yeast strain to the maximum growth phase; Hydrolyzing the cultured yeast at a temperature of 35 ° C. to 70 ° C. while simultaneously adding more proteolytic enzymes to hydrolyze the yeast protein; And Method for producing a yeast extract having a PMS and dysmenorrhea function characterized in that it comprises the step of centrifuging the hydrolyzed to separate only the supernatant. delete 10. The method according to claim 8 or 9, further comprising spray drying the obtained supernatant. Functional drink for alleviating PMS and dysmenorrhea, characterized in that it comprises a yeast extract according to any one of claims 1 to 4.