KR100386120B1 - Composition of essential oil having a protecting power against insects and plant pathogenic fungi and an antioxidant activity and product containing thereof for preserving the quality of agricultural products - Google Patents

Abstract

The present invention relates to a natural vegetable essential oil composition having an insect repellent, antibacterial and antioxidant effect and a storage agricultural product quality preservative comprising the same, in particular one or more substances selected from the group consisting of mustard essential oil, thyme essential oil and ginger essential oil, respectively, 0.01 The present invention relates to a natural vegetable essential oil composition having insect repellent, antibacterial and anti-oxidant effect, and to a storage agricultural product quality preservative comprising the composition, wherein the storage agricultural product quality preservative comprising the natural vegetable essential oil composition according to the present invention is a conventional product. Compared to the improved insect repellent, antibacterial and antioxidant effects, and does not cause environmental pollution, there is an environmentally friendly advantage.

Description

Composition of essential oil having a protecting power against insects and plant pathogenic fungi and an antioxidant activity and product containing particular for preserving the quality of agricultural products }

The present invention relates to a natural vegetable essential oil composition having an insect repellent, antibacterial and antioxidant effect and a storage agricultural product quality preservative comprising the same, in particular one or more substances selected from the group consisting of mustard essential oil, thyme essential oil and ginger essential oil, respectively, 0.01 It relates to a natural vegetable essential oil composition having an insect repellent, antibacterial and antioxidant effect containing ˜100% by weight and a storage agricultural product quality preservative comprising the composition.

Rice is a fruit of rice and herbaceous annual herb, which is usually cultivated once a year in Korea. It is harvested, threshed and dried to be processed, preserved and distributed in grain, brown rice or rice. The grain of rice is stored in a ventilated synthetic resin bag for a long time in the state of being dried up to 15% of moisture at the time of government purchase, and then stored in storage for a long time, and then processed sequentially and consumed by the public. The polished rice is usually packed in kraft paper bags or vinyl-based synthetic resin bags, usually in 20 kg units, and delivered to homes through distribution channels such as battles and shops, which are then opened and consumed or stored in rice containers.

Generally, when rice is preserved in the state of grain or brown rice, it is known that the quality of rice is not severely degraded for about a year. However, when polished, the rate of quality deterioration is so rapid that rice is severely damaged during the month. At this time, the biggest causes of deterioration of the quality of the polished rice are pests generated from stored rice, such as rice weevil, rice thief, and Hwaranggok moth. These pests multiply rapidly in rice bags or rice barrels, which not only eat nutrients and excrete in rice, but also deteriorate the quality of rice by spreading mold on the body. In addition, as the humidity in the air increases, molds and bacteria such as yellow bacteria and microorganisms that float in the air may multiply in rice bags or rice barrels, thereby accelerating the deterioration of rice quality.

In addition, the rice stored in a closed state for a long time is generally said to be a so-called scent as the temperature rises, which is due to the action of lipase and amylase of the rice components. In other words, the two components decompose the free fatty acids contained in the rice to increase the reducing sugar and thereby promote the production of aldehydes. Therefore, in order to preserve rice for a long time, it is necessary to suppress oxidation.

As a product to prevent the deterioration of the quality of the rice by preventing the breeding of rice worms generated in the rice barrels, the conventional ones, a method of installing a far-infrared radiation plate and a ventilator in the thick plate inside the rice barrel (Korean Utility Model Application No. 98-56062), a method of preventing corruption of rice by physically circulating stagnant rice by introducing an electrically driven power transmission device into the rice container (Korean Patent Application Nos. 98-26678, 98-26679, 98-26680, 98-26681), and a method for combating rice worms by installing ultraviolet sterilization lamps in rice barrels (Korean Patent Application No. 97-19996). However, the above methods are not practical due to economic problems due to power consumption as well as problems such as noise generation and temperature increase in the rice barrel.

In addition, as a folk remedy, garlic, pepper, charcoal, etc. are put in a small bag of air permeability and put in a rice container to suppress the occurrence of rice bugs or to control the humidity. However, when garlic, pepper, etc. are put directly into the rice barrel, the mites and mites attached to the garlic and pepper become more prevalent, rather, they accelerate the deterioration of rice quality. Moreover, garlic or red pepper was not added directly.

In 1998, Aekyung Co., Ltd. improved the folk remedies as described above, formulated a mixture of essential oils of red pepper and garlic, and sold rice bug repellent products under the trademark 'Dr. Rice Worm'.In 1999, LG (JP 2660196) ) And Oxy Co., Ltd. also develop products and sell them under the trademarks `` rice worm 119 '' and `` rice worm eating hippo ''. However, these products not only have a very pungent odor, but also have a low insect repellent effect. Moreover, the persistence of the insect repellent effect is also low, so the situation has been ignored by consumers.

In addition to long-term storage of not only rice, but also other stored agricultural products such as barley, corn, soybeans, red beans, buckwheat or dried and dried agricultural products such as dried red pepper, dried garlic, dried shiitake mushrooms, dried herbs, and food ingredients, There is a need to develop quality proven preservatives that can be reliably and effectively maintained during distribution, processing, preservation and consumption.

In recent years, as agricultural trade is liberalized, imported agricultural products are increasing. Imported pests, which have been incorporated into imported agricultural products, have settled in Korea, causing enormous damage to the production and distribution of agricultural products. On the other hand, in order to prevent damage caused by pests on such a relatively large storage agricultural products, fumigants such as methyl bromide (CH ₃ Br), aluminum phosphide, and chlorpicrin (Cl ₃ CNO ₂ ) are generally used. However, since these substances are very toxic to the human body and cause secondary environmental pollution problems such as destroying the ozone layer, it is urgent to develop alternative materials thereof.

Accordingly, the present inventors have tried to solve the above problems to develop excellent storage product quality preservatives, as a result, mustard essential oil, thyme essential oil and ginger essential oil found that excellent insect repellent, antibacterial and antioxidant effect, vegetable essential oil made of these natural vegetable essential oils The present invention has been completed by finding that the control effect of the stored agricultural product quality preservative comprising the composition is significantly improved compared to the conventional product.

It is an object of the present invention to provide natural vegetable essential oil compositions having insect repellent, antibacterial and antioxidant effects.

It is also an object of the present invention to provide a storage agricultural product quality preservative having excellent insect repellent, antibacterial and antioxidant effects, and a method for preparing the same, comprising the natural vegetable essential oil composition.

1 is a schematic diagram showing an example of the commercialization of the stored agricultural product quality preservative in the form of a blend composition according to the present invention,

Figure 2 is a schematic diagram showing an example of the commercialization of the stored agricultural product quality preservative in the form adsorbed on the porous mineral material according to the present invention,

Figure 3 is a schematic diagram showing an example of the commercialization of the stored agricultural product quality preservatives in the liquid form,

4 is a gas chromatogram of a vegetable natural essential oil composition according to the present invention,

Figure 5 shows the growth inhibition of S. aureus bacteria by the plant natural essential oil composition according to the present invention,

Left Shaale: Control

Right shale: 10 μl application group of vegetable natural essential oil composition according to the present invention

6a shows the quality after 60 days of inoculation of rice weevil adult in rice,

Figure 6b shows the quality after 60 days of inoculating rice weevil adult rice in rice and applying the storage agricultural product quality preservative of Example 1,

Figure 6c shows the quality after 60 days of inoculation of rice weevil adult in rice and doctor rice bug ^R.

※ Brief Description of Drawings ※

1: storage agricultural product quality preservative 5: cover

2: molded article container 6: breathable nonwoven fabric

3: lid 7: non-breathable bag

4: vent hole

In order to achieve the above object, in the present invention, natural vegetable essential oil having insect repellent, antibacterial and antioxidant effects, each containing 0.01 to 100% by weight of at least one substance selected from the group consisting of mustard essential oil, thyme essential oil and ginger essential oil. To provide a composition.

In another aspect, the present invention provides a storage agricultural product quality preservative, comprising the natural vegetable essential oil composition.

In another aspect, the present invention provides a method for producing the stored agricultural product quality preservative.

Hereinafter, the present invention will be described in detail.

The inventors searched for various plant essential oils and found that the insecticide, antibacterial and antioxidant effects of mustard essential oil, thyme essential oil and ginger essential oil are excellent. Mustard essential oil, thyme essential oil and ginger essential oil are not only pests such as rice weevil ( Sitophilus oryzae ), Tribolium confusum and Plodia interpunctella , but also yellowing pathogen ( Penicillium islandicum ) which causes yellowing of rice. It also shows excellent control effects against fungi. Thus, the essential oil component can be used as a storage agricultural product quality preservative. Moreover, unlike chemicals such as methyl bromide, aluminum phosphide, and chlorpicrine, which have been used as preservatives for agricultural products in the past, they are not toxic to humans and do not cause environmental pollution.

Specifically, in the present invention, as a preferred composition comprising the essential oil component, must have at least one substance selected from the group consisting of mustard essential oil, thyme essential oil and ginger essential oil, each having a natural insect repellent, antibacterial and antioxidant effect containing 0.01 to 100% by weight. It provides a vegetable essential oil composition.

Storage agricultural product quality preservatives comprising the natural vegetable essential oil composition may be modified in various ways its components and forms. The natural vegetable essential oil composition can also be impregnated with conventional matrices such as paper, cotton, cotton, resins, porous ceramics, silica, calcium carbonate (CaCO ₃ ), clay and the like.

An example of a preferred storage agricultural product preservative according to the present invention is a blend composition in which the natural vegetable essential oil composition is physically or mechanically formulated into a carrier to impart a sustained release effect. The storage agricultural product quality preservative of the form as described above is an extended release to suppress the volatility of the essential oil component has the advantage of excellent sustainability of the drug.

Specifically, the storage agricultural product quality preservative according to the present invention preferably contains 0.01 to 50% by weight of the natural vegetable essential oil composition and 50 to 99.9% by weight of the carrier. At this time, the carrier is preferably an absorbent, oil absorbent polymer (absorbent polymer), and the absorbent, oil absorbent polymer may be selected from a variety of polymers or monomers, and can be manufactured to be absorbent and oil absorbent all materials can be prepared Include. As the absorbent and oil absorbent polymer, natural, semi-synthetic, synthetic materials may be used. Specifically, for example, the natural polymer may include starch, agar, guar gum, cyamoposis gum, carob locust bean gum, waxes (eg, solid paraffin, etc.). Semi-synthetic polymers include cellulose, cellulose derivatives (e.g., hydroxypropyl cellulose, etc.), sodium alginate, cyclodextrin, crosslinked carboxymethylcellulose (CMC), and the like. The synthetic polymers include EVA (ethyl vinyl acetate) resin, urethane resin, acrylic resin, silicone resin, polyvinyl chloride resin, PVA (polyvinyl alcohol) resin, PEO (polyethylene oxide) resin, starch resin, PVP ( polyvinyl pyrrolidone resin, PP (polypropylene) resin, polyacrylate resin, polyacrylamide, polyacrylic acid ester partial saponification, polyacrylonitrile saponification (saponified polyacrylonitrile), Alkylstyrene-divinyl benzene (DVB) crosslinked polymer, polynorbornene, starch-acrylic acid graft copolymer, starch saccharin-polyacrylonitrile copolymer, PVA-acrylate Copolymers, isobutylene-maleic acid copolymers, rubbers (e.g. silicone rubber, chloroprene rubber, butyl rubber, styrene-butadiene rubber (SBR), ethylene-propylene-diene rubber , EPDM), natural rubber, and the like).

In addition, when the carrier is a silicone rubber, it is preferable to include 0.01 to 30% by weight of a curing agent in addition to the above components. In this case, as the curing agent, a conventional curing agent having deep curing property can be used, and in particular, it is preferable to use a diorganotin compound, and for example, it is preferable to use a silicone curing agent.

Another preferred storage agricultural product quality preservative according to the present invention is a form in which the natural vegetable essential oil composition is adsorbed on a porous oil with high oil absorption.

Specifically, the storage agricultural product quality preservative according to the present invention preferably contains 0.01 to 50% by weight of the natural vegetable essential oil composition and 50 to 99.9% by weight of the porous mineral. In this case, the porous mineral material is preferably selected from the group consisting of zeolite, perlite, porous ceramic, silica, calcium carbonate (CaCO ₃ ) and clay.

Another preferred storage agricultural product quality preservative according to the present invention is in liquid form in which the natural vegetable essential oil composition is mixed with a carrier oil.

Specifically, the low quality agricultural product preservative according to the present invention preferably contains 0.01 to 50% by weight of the natural vegetable essential oil composition and 50 to 99.9% by weight of carrier oil. At this time, the carrier oil may be olive oil, tocopherol, coconut oil, jojoba oil, sweet almond oil, palm oil and linda base oil ( preferably linda-base oil).

According to the present invention, the stored agricultural product quality preservative including the natural vegetable essential oil composition may be prepared by a conventional manufacturing method appropriate for each component and form.

Specifically, the storage agricultural product quality preservative according to the present invention

1) mixing and uniformly dispersing the natural vegetable essential oil composition 0.01 to 50% by weight and the carrier 50 to 99.9% by weight (step 1); And

2) it can be prepared by the step (step 3) of the mixture of step 2 is injected into a molded container and cured.

When the carrier is a kind other than the silicone rubber mentioned above, it may be cured by changing the temperature or the concentration of the carrier. In the case where the carrier is a silicone rubber, it is preferable to add 0.01 to 30% by weight of a curing agent to the mixture of Step 1 to uniformly mix the mixture, and then inject the mixture into a molded container to cure.

In addition, the storage agricultural product quality preservative according to the present invention can be prepared in the form adsorbed on the porous mineral by spraying the natural vegetable essential oil composition to the porous mineral.

In addition, the storage agricultural product quality preservative according to the present invention can be prepared in liquid form by mixing 0.01 to 50% by weight of the natural vegetable essential oil composition and 50 to 99.9% by weight of carrier oil.

1, 2, and 3 illustrate examples of forms in which the stored agricultural product quality preservative according to the present invention is conveniently used.

FIG. 1 shows an example of commercializing a storage produce quality preservative in the form of the blend composition, wherein the preservative (1) is contained in a resin molded article or a can or container made of iron or aluminum, and has a lid having a vent hole (4). A cover 5 is provided to prevent the fragrance component of the preservative from falling out after opening 3). The resin molded article may be a molded article made of, for example, an olefin-based, polystyrene-based, acrylic resin, or biodegradable resin, but is not limited thereto. A can of a material other than steel or aluminum cans may be used.

Figure 2 shows an example in which the natural vegetable essential oil composition commercialized the storage agricultural product quality preservative in the form adsorbed on the porous mineral. The storage agricultural product quality preservative 1 is contained in a bag made of a nonwoven fabric 6 coated with breathable vinyl therein. Since the inside of the nonwoven fabric 6 is coated with breathable vinyl, the aromatic component of the preservative may be diffused to the outside through the nonwoven fabric. The non-woven fabric can be used in general commercial products, there is a low cost and environmentally friendly advantages.

Figure 3 shows an example of the commercialization of the storage product quality preservatives in liquid form in which the carrier oil and the natural vegetable essential oil composition are mixed. The storage agricultural product quality preservative 1 is put in a bag 7 which is not breathable, and the bag 7 which is not breathable is put again in a bag made of a nonwoven fabric 6 coated with breathable vinyl therein. The nonwoven fabric 6 does not pass the liquid, but since the breathable vinyl is coated therein, the fragrance component of the preservative may be diffused to the outside through the nonwoven fabric. In this double packaged product form, a non-breathable bag (7) is pressurized immediately before use to expel the stored agricultural produce preservative in liquid form to the outside of the non-breathable bag.

1, 2 and 3, but the product form of the storage agricultural product quality preservative according to the present invention has been described, but this is only to illustrate an example, the present invention is not limited thereto.

On the other hand, the storage agricultural product quality preservative of the present invention produced in the form as described above is excellent in the control effect and the sustainability of the drug compared to the products that are commercially available, the irritating odor is also significantly reduced.

Hereinafter, the present invention will be described in more detail with reference to Examples.

However, the following examples are illustrative of the present invention, and the content of the present invention is not limited by the examples.

Example 1 Search for Plant Essential Oil

In order to search for vegetable natural essential oils that have insecticidal and bactericidal and antioxidative effects and have medical benefits in terms of medicine, the following methods are used to find the greatest damage to the deterioration of the quality of stored grains such as rice. The state examined the remedies and avoidance effects of representative grain crops.

To examine the insecticidal and repelling effects of Sitophilus oryzae adults, Tribolium confusum adults, and Plodia interpunctella adults raised at the screening center of the Dongbu Institute of Technology received from the Department of Agricultural Biology, Chungbuk National University. It was.

Five kilograms of commercially purchased fresh rice was contained in a rectangular plastic container with a total internal volume of 40 liters, and then 100 seedlings were inoculated into three different barrels of each of the three low-growing pests. It was treated with mustard extract (Nippon Terpene Chemical Co., Japan), thyme extract (Hemoji Co., Japan) and ginger extract (Raiderts Co., USA) with vegetable natural essential oils, respectively, 50 μl, 100 μl, 200 μl. . The mortality and evasion rate were examined after 1, 5 and 15 days of treatment with vegetable natural essential oils, respectively. The mortality rate was examined from dead worms in the barrel, and the evasion rate was from the appendages that fled into the gaps of the container lids. In addition, the experiment was repeated three times for each treatment, the results are shown in Table 1 and Table 2 as the average.

Mortality Plant essential oil Throughput (μl / 40l) Mortality rate (%) 1 day after processing 5 days after treatment 15 days after treatment A B C A B C A B C Mustard Extract 50 0 0 0 19 0 10 30 0 10 100 6 0 10 94 0 60 97 30 65 200 100 0 70 100 5 70 100 70 80 Thyme Extract 50 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 15 0 200 0 0 0 0 10 0 20 35 15 Ginger Extract 50 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 200 0 0 0 0 0 0 15 0 10 A: Rice weevil B: Hwaranggok moth C: Silly rice thief

Evasion rate Plant essential oil Throughput (μl / 40l) Evasion rate (%) 1 day after processing 5 days after treatment 15 days after treatment A B C A B C A B C Mustard Extract 50 10 0 0 15 0 10 30 10 10 100 35 10 25 40 25 25 40 55 40 200 - 60 40 - 75 50 - 80 50 Thyme Extract 50 0 0 0 0 0 0 0 0 0 100 0 0 0 0 5 0 10 25 10 200 0 0 0 0 15 0 25 40 30 Ginger Extract 50 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 200 0 0 0 0 0 0 30 20 20 A: Rice weevil B: Hwaranggok moth C: Silly rice thief

As can be seen in Table 1 and Table 2, the mustard extract, thyme extract, and ginger extract were both excellent mortality and evasion rate, and also excellent in the sustainability of the drug. In particular, the thyme extract and ginger extract began to appear a few days after the treatment, but the mustard extract began to appear immediately after treatment.

As such, mustard essential oil, thyme essential oil and ginger essential oil have excellent control and repelling effect against low-grained pests that have the greatest damage to the quality of storage grains, such as rice, it can be used as storage agricultural product quality preservatives.

Example 2 Analysis of Natural Vegetable Essential Oil Composition

Component analysis was carried out for a mixed composition consisting of mustard essential oil, thyme essential oil and ginger essential oil.

The composition was analyzed using gas chromatography (Hewlett Packard, HP 6890 series GC system, column: J & W DB-5MS, length: 30 m, internal diameter: 0.25 mm) over a total elution time of about 33 minutes. Mass spectrometry was performed. Each component of the composition was also analyzed with reference to gas chromatograms for standard materials. The gas chromatogram of the composition of the present invention is shown in Figure 4, the components corresponding to each elution time is shown in Table 3 below.

Assay of natural vegetable essential oil composition Elution time (minutes) Analyte 3.79 Allyl iso thio cyanate 4.75 Camphene, etc. 5.85 o-cymeme 6.38 γ-terpinene 9.89 Thymol 12.38 ar-curcumene (ar-curcumene) 12.58 α-zingiberenee, e-α-farnesene 12.72 β-bisaboline (β-bisabolene) and the like 12.93 β-sesquiphellandrene

Example 3 Preparation of Storage Agricultural Products Quality Preservative 1

A storage agricultural product quality preservative comprising a natural vegetable essential oil composition according to the present invention was prepared by the following method.

4.5 g of an essential oil composition consisting of 1% by weight of mustard essential oil, 1% by weight of thyme essential oil and 2.5% by weight of ginger essential oil was mixed with 94.5 g of silicone room temperature vulcanizing (RTV) rubber (KE 12 , Shin-Etsu Silicone Co., Ltd.) By mixing evenly. 1.0 g of a silicone curing agent (CAT-RM, Shin-Etsu Silicone Co., Ltd.) was added to the mixture, and the mixture was uniformly mixed again. Before the mixture was cured, the product was prepared by placing it in a molding container having a desired shape in advance using a machine that is quantitatively dispensed and curing at room temperature.

Example 4 Preparation of Storage Agricultural Products Quality Preservative 2

A storage agricultural product quality preservative comprising a natural vegetable essential oil composition according to the present invention was prepared by the following method.

4.5 g of an essential oil composition consisting of 1% by weight of mustard essential oil, 1% by weight of thyme essential oil and 2.5% by weight of ginger essential oil was uniformly sprayed onto 95.5 g of zeolite (10 oil absorption, Dongbu Hannong Chemical Co., Ltd.) and adsorbed. The stored agricultural product quality preservative in the form adsorbed on the zeolite thus prepared was sealed in a small bag made of a conventional nonwoven fabric coated with breathable vinyl therein.

Example 5 Preparation of Storage Agricultural Products Quality Preservative 3

A storage agricultural product quality preservative comprising a natural vegetable essential oil composition according to the present invention was prepared by the following method.

4.5 g of an essential oil composition consisting of 1% by weight mustard essential oil, 1% by weight thyme essential oil and 2.5% by weight ginger essential oil was uniformly mixed with 95.5 g of olive oil. The liquid agricultural product preservative in the liquid form thus prepared was first sealed in a non-breathable plastic bag and then sealed in a bag made of a nonwoven fabric coated with a breathable vinyl to prepare a double-packed form.

Experimental Example 1 Control Effect Experiment of Natural Plant Essential Oil Composition

In order to determine the extent to which the natural plant essential oil composition according to the present invention inhibits the growth of yellow bacteria ( Penicillium islandicum KCTC 6931), a pathogen causing yellowing rot of rice, the following experiment was performed.

Yellow microorganism was distributed by the strain gene bank (KCTC) in the Korea Institute of Biotechnology and used for the experiment. Potassium agar medium (PDA) was inoculated with yellow bacteria in a Petri dish of 9 cm in diameter and incubated for 10 days in a thermostat controlled at 24 ℃ to form spores. 10 ml of sterile distilled water was put on the fully grown mycelium, scraped with a brush to collect mycelium and spores, and then filtered with four layers of gauze to prepare a pure spore suspension. The spore suspension was evenly mixed in PDA medium which had been sterilized in advance and cooled to about 45 ° C., and then 20 ml of a 9 cm diameter Petri dish was cured at room temperature for 1 hour. A filter paper disk with a diameter of 8 mm was placed in the center of this Petri dish, and 10 µl of a natural plant essential oil composition consisting of mustard essential oil: thyme essential oil: ginger essential oil = 1: 1: 2.5 (weight ratio) was treated. This was incubated for 5 days in a 24 ℃ thermostat, and compared with the mycelial growth without treatment. The results are shown in FIG.

As can be seen in Figure 5, in the control group yellow bacteria grow and multiply well, but when the natural plant essential oil composition according to the present invention yellow bacteria did not multiply. Therefore, it was found that the natural plant essential oil composition according to the present invention exhibits not only an insect repellent effect but also an antibacterial effect against the fungus.

Experimental Example 2 Control Effect of Preserved Agricultural Products Quality Preservative

In order to investigate the control effect of the rice weevil adult, young rice thief adult and Hwaranggok moth adult of the stored agricultural product quality preservative according to the present invention, the following experiments on the products prepared in Examples 3 to 5 Was carried out.

To examine the insecticidal and repelling effects of Sitophilus oryzae adults, Tribolium confusum adults, and Plodia interpunctella adults raised at the screening center of the Dongbu Institute of Technology received from the Department of Agricultural Biology, Chungbuk National University. It was.

After 5 kg of commercially purchased fresh rice was placed in a rectangular plastic container with a total internal volume of 40 L, the three grain pests in the breeding were inoculated into 100 different barrels. The stored agricultural product quality preservative prepared in Examples 3-5 was put there. In addition, as a comparative example, commercially available doctor rice worm ^R (Aekyung Co., Ltd.), rice worm 119 ^R (LG Co., Ltd.) and rice worm-eating hippo ^R (Oxy Co., Ltd.) were placed in different containers.

The mortality and evasion rate were investigated after 10 days, 30 days, 60 days, and 90 days of storing agricultural product quality preservatives while continuously inoculating 100 grain pests every 10 days. Same as 2. In addition, the experiment was repeated three times for each treatment, the results are shown in Table 4, Table 5 and Table 6 below as an average.

Control Effect on Rice Weevil Preservation of Agricultural Product Quality Control Effect on Rice Weevil Mortality rate (%) Evasion rate (%) 10 days later 30 days later 60 days later 90 days later 10 days later 30 days later 60 days later 90 days later Example 3 100 40 20 10 - 90 85 60 Example 4 90 10 5 0 0 70 55 20 Example 5 95 20 10 0 0 25 15 0 Doctor Rice Bug ^R 0 14 0 - 0 30 20 - Rice Worm 119 ^R 0 5 0 - 0 20 5 - Rice Worm Eating Hippo ^R 10 2 0 - 0 10 5 -

Control Effect on Hwaranggok Moth Preservation of Agricultural Product Quality Control Effect on Hwagok Moth Mortality rate (%) Evasion rate (%) 10 days later 30 days later 60 days later 10 days later 30 days later 60 days later Example 3 60 35 12 85 70 41 Doctor Rice Bug ^R 40 20 10 74 25 20 Rice Worm 119 ^R 0 0 0 47 30 15 Rice Worm Eating Hippo ^R 0 0 0 45 24 5

Control effect on young rice thief Preservation of Agricultural Product Quality Control Effects Against Foolish Thieves Mortality rate (%) Evasion rate (%) 10 days later 30 days later 60 days later 10 days later 30 days later 60 days later Example 3 85 50 30 90 74 45 Doctor Rice Bug ^R 35 0 0 63 37 0 Rice Worm 119 ^R 0 0 0 0 30 0 Rice Worm Eating Hippo ^R 0 0 0 0 15 0

As can be seen in Table 4 to Table 6, the storage agricultural product quality preservative according to the present invention was superior to the control effect compared to the conventional product.

Specifically, while for rice weevil, the conventional products have a mortality rate and avoidance rate of less than 50%, while the storage agricultural product quality preservatives according to the present invention all showed an improved control effect. The stored agricultural product quality preservative of Example 3 had a mortality rate of 100% after 10 days of treatment and a 90% avoidance rate after 30 days of treatment. In particular, the conventional products, the control effect was not maintained continuously, but all of the stored agricultural product quality preservatives according to the present invention showed a continuous control effect for more than 90 days.

In addition, the preservation quality of the stored agricultural products according to the present invention showed improved mortality and evasion rate against the Hwaranggok moth and young rice thief, in particular, the control effect was maintained for a long time.

As described above, the conventional products are not only effective in controlling the effects, but the effects are not sustained. However, the storage agricultural product quality preservatives including the natural vegetable essential oil composition according to the present invention have been found to have improved control effects and sustained effects. Could.

Experimental Example 3 Preservation Effect of Preserved Agricultural Products

In order to examine the control effect of the pests of the stored agricultural product quality preservation according to the present invention, an experiment was performed to compare the amount of reduced rice by the rice weevil damage.

Specific experimental conditions are the same as in Experimental Example 2, and the pests were inoculated with rice weevil and the weight of the rice was measured before and 60 days after treatment with the stored agricultural product quality preservative. Since rice is broken into powder when damaged by low-pest insects, the powder was separated by sieving with a 1.4 mm pore size before weighing the rice. Each treatment was repeated three times and the average was calculated. The results are shown in Table 7 below.

Change in weight of rice Storage Agricultural Products Quality Preservative First Rice Weight (kg) Weight of rice after 60 days (kg) Reduction Rate (%) Example 3 5.0 5.0 0 Doctor Rice Bug ^R 5.0 4.6 8 Rice Worm 119 ^R 5.0 4.4 12 Rice Worm Eating Hippo ^R 5.0 4.5 10

As can be seen in Table 7, the conventional products were found to be unable to use a considerable amount of rice turned to powder due to damage by rice weevil after 60 days. In the case of rice worm 119 ^R , after 60 days, it turned to 12% of the total weight of the rice. On the other hand, the storage product quality preservative according to the present invention was not reduced at all after 60 days, it was found that the damage was not affected by rice weevil, the results are shown in Figure 6a, 6b, 6c.

Experimental Example 4 Odor Stimulation Experiment of Preserved Agricultural Products Quality Preservative

In order to find out how irritating the smell of the stored agricultural product quality preservative according to the present invention, a comparative experiment with a conventional product was conducted.

The products of the stored agricultural product quality preservatives prepared in Examples 3 to 5 and Dr. Rice Worm ^R , which are currently commercialized, were not covered, and the products could not be discriminated. Odor irritation was represented by five integers from 5 to 1 from very severe to very mild cases, the results are shown in Table 8 below.

Odor irritant Storage Agricultural Products Quality Preservative score Example 3 2.5 Example 4 1.9 Example 5 2.0 Doctor Rice Bug ^R 4.6

As can be seen from Table 8, the storage agricultural product quality preservative according to the present invention was significantly purified odor irritation compared to the conventional product. Thus, the stored produce quality preservatives of the present invention are much less likely to be avoided from consumers due to the irritating odor.

Experimental Example 5 Antioxidant Effect of Natural Plant Essential Oils

In order to find out the antioxidant activity of the natural plant essential oil according to the present invention, the following experiment was carried out.

Brains were isolated from fetuses of rats 18 days pregnant and the nerve cells were cultured for 7 days. Nerve cell cultures were washed twice with serum-free MEM (Minimal Essential Media) medium and then treated with mustard essential oil, thyme essential oil and ginger essential oil, 10 μM and 50 μM, respectively. After 30 minutes, 50 μM of FeSO ₄ was treated as an oxidizing agent to induce oxidation. After 24 hours, 50 μl of each nerve cell culture treated with the test substance was transferred to a 96-well plate and a substrate mixture provided by an LDH kit (Promega, Cyto Tox96 Non-radioactive Cytotoxicity Assay). 50 μl of the substrate mixture was added and stored at room temperature under dark conditions for 30 minutes. After 30 minutes, 50 μl of the stop solution provided by the LDH kit was added to the culture medium to stop the reaction, and the LDH (lactate dehydrogenase) concentration was measured by measuring the optical density (OD) at 490 nm with an ELISA reader. .

From the concentration of LDH, the antioxidant effect (%) of each essential oil was calculated by the following Equation 3, and the results are shown in Table 9 below.

Antioxidant Effects of Natural Plant Essential Oils Control FeSO ₄ Mustard essential oil Thyme essential oil Ginger essential oil Dosage - 50 μM 10 μM 50 μM 10 μM 50 μM 10 μM 50 μM O.D. 0.212 0.427 0.376 0.354 0.396 0.389 0.346 0.287 Antioxidative effect (%) - - 24 34 14 18 38 65

As can be seen in Table 9, the natural plant essential oils according to the present invention all exhibited antioxidant activity. In particular, thyme essential oil had low antioxidant activity, but mustard essential oil and ginger essential oil had excellent antioxidant activity. Therefore, the natural plant essential oil composition according to the present invention and the storage product quality preservative containing the same may be used for the purpose of preventing oxidation of the storage product in addition to the antibacterial and insect repellent effect.

As described above, since the natural vegetable essential oil composition containing 0.01 to 100% by weight each of one or more substances selected from the group consisting of mustard essential oil, thyme essential oil and ginger essential oil according to the present invention is excellent in insect repellent, antibacterial and antioxidant effect In addition, it can be usefully used as a preservation quality of stored agricultural products to prevent the damage of cereals to low grain pests. Moreover, the stored agricultural product quality preservative according to the present invention has excellent sustainability of the drug and reduced irritant odor.

Claims (13)

1) 0.01-49.99 wt% of a natural vegetable mixed essential oil composition comprising 0.01-99.98 wt% of mustard essential oil, thyme essential oil and ginger essential oil, 2) 50-99.9 wt% of silicone rubber, and 3) 0.01-30 wt% of silicone curing agent. A storage agricultural product quality preservative obtained by adding and mixing and curing in a molding container. 1) preparing a natural vegetable mixed essential oil composition comprising mustard essential oil, thyme essential oil and ginger essential oil, 2) adding a silicone rubber and a silicone curing agent to the mixed essential oil composition to uniformly mix, and 3) A method for preparing a stored agricultural product quality preservative according to claim 1, comprising the step of injecting and curing the mixture obtained in step 2) into a molding container. delete delete delete delete delete delete delete delete delete delete delete