KR100363615B1 - rpoB 유전자 단편 및 이를 이용한 결핵균과 비결핵마이코박테리아의 동정방법 - Google Patents
rpoB 유전자 단편 및 이를 이용한 결핵균과 비결핵마이코박테리아의 동정방법 Download PDFInfo
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- KR100363615B1 KR100363615B1 KR1019990046795A KR19990046795A KR100363615B1 KR 100363615 B1 KR100363615 B1 KR 100363615B1 KR 1019990046795 A KR1019990046795 A KR 1019990046795A KR 19990046795 A KR19990046795 A KR 19990046795A KR 100363615 B1 KR100363615 B1 KR 100363615B1
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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Abstract
Description
균 주 | ATCC NO. |
마이코박테리움 앱세수스M. abscessus 마이코박테리움 아시아티쿰(M.asiaticum) 마이코박테리움 아비움(M. avium) 마이코박테리움 아이키엔스(M. aichiense) 마이코박테리움 아우룸(M. aurum) | 25276252912728023366 |
마이코박테리움 아프리카눔(M. africanum) 마이코박테리움 오스트로아프리카눔(M. austroafricanum) 마이코박테리움 보비스(M. bovis) 마이코박테리움 보비스 BCG(M. bovisBCG) 마이코박테리움 첼로네(M. chelonae) | 254201921035749 |
마이코박테리움 셀라툼(M. celatum) 마이코박테리움 플라베센스(M. flavecense) 마이코박테리움 포르투이툼(M. fortuitum) 마이코박테리움 갈리나룸(M. gallinarum) 마이코박테리움 길붐(M. gilvum) | 51131684143909 |
마이코박테리움 고르도네(M. gordonae) 마이코박테리움 가스트리(M. gastri) 마이코박테리움 헤모필룸(M. haemophilum) 마이코박테리움 인트라셀루라레(M. intracellulare) 마이코박테리움 칸사시이(M. kansasii) | 1447015754295841395012478 |
마이코박테리움 말모엔스(M. malmoense) 마이코박테리움 마리눔(M. marinum) 마이코박테리움 모리오케세(M. moriokaese) 마이코박테리움 페레그리눔(M. peregrinum) 마이코박테리움 플레이(M. phlei) | 295719271446711758 |
마이코박테리움 풀베리스(M. pulveris) 마이코박테리움 스크로풀라세움(M. scrofulaceum) 마이코박테리움 스줄가이(M. szulgai) 마이코박테리움 스메그마티스(M. smegmatis) 마이코박테리움 테레(M. terrae) | 199813579915755 |
마이코박테리움 투베쿨로시스(M. tuberculosis) 마이코박테리움 울서란스(M. ulcerans) 마이코박테리움 제노피(M. xenopi) 노카르디아 브라실리크로스(Nocardia brasilicros) 노카르디아 노바(Nocardia nova) 로도코커스(Rhodococcus) | 19423 |
Claims (5)
- 서열목록의 서열번호(SEQ. ID. NO.) 1 내지 4 또는 6 내지 24로 표시된 염기서열중 어느 하나를 갖는 유전자 단편.
- 다음 단계:(1)서열목록의 서열번호(SEQ. ID. NO.) 1 내지 24로 표시된 염기서열중 어느 하나를 갖는 유전자 단편을, 제한효소 Msp Ⅰ, Hae Ⅲ, Sau 3A1 및 Bst EⅡ로부터 구성된 군으로부터 선택된 적어도 1종 이상의 제한효소로 절단하여 얻어진 단편들의 길이 다형성(length polymorphism) 패턴을 얻는 단계:(2) 동정하고자 하는 미생물로부터 DNA를 분리하는 단계:(3)서열번호 25 및 26에 기재된 서열을 갖는 프라이머를 이용하여 PCR 증폭함으로써, 상기 단계 (2)의 DNA로부터 rpoB 유전자 단편을 증폭시키는 단계:(4) 상기 증폭된 DNA를 상기 단계 (1)에서와 동일한 제한효소로 절단하는 단계:(5) 상기 단계 (4)에서 얻어진 제한효소 절단 단편들의 길이 다형성(length polymorphism) 패턴을 얻는 단계: 및(6) 상기 단계 (5)에서 얻어진 패턴과 상기 단계 (1)에서 얻어진 패턴을 비교하는 단계를 포함함을 특징으로 하는 마이코박테리아(Mycobacteria) 속 균주의 동정방법.
- 제 2항에 있어서, 상기 단계 (1)과 단계 (5)의 길이 다형성 패턴은 전기영동에 의해 얻는 것을 특징으로 하는 방법.
- 삭제
- 제 2항 내지 제4항중 어느 한항에 있어서, 상기 마이코박테리아 속 균주는 결핵균(Mycobacterium tuberculosis), 마이코박테리움 아비움(M. avium), 마이코박테리움 앱세수스(M. abscessus), 마이코박테리움 플라베센스(M. flavescence), 마이코박테리움 아프리카눔(M. africanum), 마이코박테리움 보비스(M. bovis), 마이코박테리움 첼로네(M. chelonae), 마이코박테리움 셀라툼(M. celatum), 마이코박테리움 포르투이툼(M. fortuitum), 마이코박테리움 고르도네(M. gordonae), 마이코박테리움 가스트리(M. gastri), 마이코박테리움 헤모필룸(M. haemophilum), 마이코박테리움 인트라셀루라레(M. intracellulare), 마이코박테리움 칸사시이(M. kansasii), 마이코박테리움 말모엔스(M. malmoense), 마이코박테리움 마리눔(M. marinum), 마이코박테리움 스줄가이(M. szulgai), 마이코박테리움 테레(M. terrae), 마이코박테리움 스크로풀라세움(M. scrofulaceum), 마이코박테리움 울서란스(M. ulcerans)또는마이코박테리움 제노피(M. xenopi)임을 특징으로 하는 방법.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019990046795A KR100363615B1 (ko) | 1999-10-27 | 1999-10-27 | rpoB 유전자 단편 및 이를 이용한 결핵균과 비결핵마이코박테리아의 동정방법 |
JP2001533196A JP4637430B2 (ja) | 1999-10-27 | 2000-10-27 | 結核菌と非結核マイコバクテリア菌種の同定方法 |
US09/697,123 US6951718B1 (en) | 1999-10-27 | 2000-10-27 | rpoB gene fragments and a method for the diagnosis and identification of Mycobacterium tuberculosis and non-tuberculosis Mycobacterial strains |
AU10611/01A AU1061101A (en) | 1999-10-27 | 2000-10-27 | Rpob gene fragments and a method for the diagnosis and identification of mycobacterium tuberculosis and non-tuberculosis mycobacterial strains |
PCT/KR2000/001223 WO2001031061A1 (en) | 1999-10-27 | 2000-10-27 | rpoB GENE FRAGMENTS AND A METHOD FOR THE DIAGNOSIS AND IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS AND NON-TUBERCULOSIS MYCOBACTERIAL STRAINS |
EP00971867A EP1248854B1 (en) | 1999-10-27 | 2000-10-27 | rpoB GENE FRAGMENTS AND A METHOD FOR THE DIAGNOSIS AND IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS AND NON-TUBERCULOSIS MYCOBACTERIAL STRAINS |
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Application Number | Priority Date | Filing Date | Title |
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KR1019990046795A KR100363615B1 (ko) | 1999-10-27 | 1999-10-27 | rpoB 유전자 단편 및 이를 이용한 결핵균과 비결핵마이코박테리아의 동정방법 |
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KR20010038701A KR20010038701A (ko) | 2001-05-15 |
KR100363615B1 true KR100363615B1 (ko) | 2002-12-06 |
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US (1) | US6951718B1 (ko) |
EP (1) | EP1248854B1 (ko) |
JP (1) | JP4637430B2 (ko) |
KR (1) | KR100363615B1 (ko) |
AU (1) | AU1061101A (ko) |
WO (1) | WO2001031061A1 (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR100874744B1 (ko) | 2002-02-04 | 2008-12-19 | (주)바이오니아 | 레지오넬라 균종의 리보핵산 중합효소 베타 서브유닛의데옥시리보핵산 분절과 이에 선택적인 프라이머, 이를이용한 레지오넬라 균종의 판별 방법 |
Families Citing this family (5)
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KR100446850B1 (ko) * | 2001-07-19 | 2004-09-04 | 이혜영 | 마이코박테리아의 동정 및 rpoB 유전자의 돌연변이에의해 얻어지는 항 결핵제 내성의 동시 검출방법 |
US7094542B2 (en) * | 2001-09-18 | 2006-08-22 | Gen-Probe Incorporated | Detection of rpoB sequences of Mycobacterium tuberculosis |
WO2004029296A2 (en) * | 2002-09-26 | 2004-04-08 | Koeksalan Orhan Kaya | Molecular size markers for species identification of mycobacteriae |
FI115637B (fi) * | 2003-12-19 | 2005-06-15 | Mobidiag Oy | Diagnostinen menetelmä hengitystieinfektioita aiheuttavien bakteerien osoittamiseksi ja tunnistamiseksi ja menetelmässä käyttökelpoinen alukeseos |
EP2270202A1 (en) * | 2009-07-03 | 2011-01-05 | John Ikonomopoulos | Mycobacterial detection |
Citations (1)
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KR970065731A (ko) * | 1997-07-28 | 1997-10-13 | 국윤호 | 마이코박테리아의 rpoB 유전자 분절을 표적으로 하는 PCR-RFLP에 의한 마이코박테리아 균종의 탐지 및 동정방법 |
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US5643723A (en) * | 1994-05-26 | 1997-07-01 | Roche Molecular Systems, Inc. | Detection of a genetic locus encoding resistance to rifampin in mycobacterial cultures and in clinical specimens |
WO1995033851A2 (en) * | 1994-06-09 | 1995-12-14 | Innogenetics N.V. | Method for the detection of the antibiotic resistance spectrum of mycobacterium species |
JPH09103300A (ja) * | 1995-10-12 | 1997-04-22 | Touyoubou Jiin Anarishisu:Kk | 結核菌RNAポリメラーゼβサブユニット遺伝子増幅用オリゴヌクレオチドおよびその用途 |
US7108968B2 (en) * | 1998-04-03 | 2006-09-19 | Affymetrix, Inc. | Mycobacterial rpoB sequences |
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- 2000-10-27 WO PCT/KR2000/001223 patent/WO2001031061A1/en active IP Right Grant
- 2000-10-27 JP JP2001533196A patent/JP4637430B2/ja not_active Expired - Lifetime
- 2000-10-27 US US09/697,123 patent/US6951718B1/en not_active Expired - Lifetime
- 2000-10-27 EP EP00971867A patent/EP1248854B1/en not_active Expired - Lifetime
- 2000-10-27 AU AU10611/01A patent/AU1061101A/en not_active Abandoned
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KR970065731A (ko) * | 1997-07-28 | 1997-10-13 | 국윤호 | 마이코박테리아의 rpoB 유전자 분절을 표적으로 하는 PCR-RFLP에 의한 마이코박테리아 균종의 탐지 및 동정방법 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR100874744B1 (ko) | 2002-02-04 | 2008-12-19 | (주)바이오니아 | 레지오넬라 균종의 리보핵산 중합효소 베타 서브유닛의데옥시리보핵산 분절과 이에 선택적인 프라이머, 이를이용한 레지오넬라 균종의 판별 방법 |
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Publication number | Publication date |
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JP2003512084A (ja) | 2003-04-02 |
JP4637430B2 (ja) | 2011-02-23 |
AU1061101A (en) | 2001-05-08 |
KR20010038701A (ko) | 2001-05-15 |
WO2001031061A1 (en) | 2001-05-03 |
EP1248854B1 (en) | 2004-07-21 |
EP1248854A1 (en) | 2002-10-16 |
US6951718B1 (en) | 2005-10-04 |
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